CN107027819A - A kind of preparation method for the natural bactericidal agent for preventing and treating reed wormwood artemisia powdery mildew - Google Patents
A kind of preparation method for the natural bactericidal agent for preventing and treating reed wormwood artemisia powdery mildew Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/16—Ericaceae [Heath or Blueberry family], e.g. rhododendron, arbutus, pieris, cranberry or bilberry
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
Abstract
A kind of preparation method of the natural bactericidal agent for preventing and treating reed wormwood artemisia powdery mildew that the present invention is provided, is made up of following component：Garlic, bitter dish, lotus leaf, Lappula echinata, lantana, Flos Magnoliae Liliflorae, sparrow Soviet Union, mantis egg-case, a red-spotted lizard Ba Zi, Rabdosia rubescens.The natural bactericidal agent raw material sources of the preventing and treating reed wormwood artemisia powdery mildew enrich, with low cost, are made of pure natural component, not only Nonpoisonous, non-environmental-pollution, and can effectively prevent and treat reed wormwood artemisia powdery mildew.
Description
Technical field
The invention belongs to prevent and treat pest and disease damage field, more particularly to a kind of natural bactericidal agent and its system for preventing and treating reed wormwood artemisia powdery mildew
Preparation Method.
Background technology
Reed wormwood artemisia (scientific name：Artemisia selengensis Turcz.ex Bess.) it is composite family sagebruss.Young stem and leaf,
Root-like stock, also known as artemisia selengensis, water Chinese mugwort, water wormwood artemisia etc..Perennial herb；Plant has faint scent.Main root is not substantially or slightly obvious, has many
Number lateral root and fibrous fibrous root；Very shape stem is slightly thick, uprightly or obliquely, 4-10 millimeters of diameter, there is creeping underground stem.Leaf papery or
Papery, above green, hairless or near hairless, the close wool smooth by canescence spider's thread shape in the back side；The lower portion of the stem leaf width it is avette or
It is avette, totally cleaved or drastic crack closely into palmate or finger-like, 5 or 3, divide leaf sliver is linear or wire lanceolar.Capitulum is most,
Long Circle or width egg shape, and constitute on stem panicle that is narrow and extending.Achene is avette, slightly flat, and upper end occasionally has asymmetric
Corolla areloe.The flowering fruit bearing stage 7-10 months.
Reed wormwood artemisia is the herbaceos perennial of wet middle raw shade tolerance.In general 4 months of north of China, the last ten-days period sprout, and open July
Flower, late August to early September is ripe, and mid-October, plant was withered and yellow.It is born in forest, forest land grassland and flat-bottomed land more.It is common in
Sylvan life, border, gully and river valley two sides, also see Plain limes marginis, pool edge and paddy field ridge side.It is the companion of grassy marshland and Marsh meadow
Non-hibernating eggs.In some areas, such as ditch, paddy field side or pond bank can turn into sociales, or even can form the pure group of single excellent kind
Segment.Sometimes also it is scattered near cottage, roadside, Tian Bian, as common weeds.Reed wormwood artemisia has long-rhizome, forms close in underground
Collect root layer, procreation ability is also very strong on the ground, therefore, often into aggregated pattern in group.Sometimes moist climate region wasteland is turned into
The pionner of succession.
Powdery mildew is one of common disease of reed wormwood artemisia.Powdery mildew mainly causes harm blade, and petiole and stem can also fall ill.Morbidity is just
There is white subcircular amyloid plaque in phase, blade, and subsequent scab is expanded into hickie in flakes, and serious whole blade is covered with white powder, Ye Zheng
Face overweights the back side.Fall ill the later stage, white mildew is changed into grey, yellowish-brown granule in heaps is born on scab, last granule becomes
Into black.
At present, for preventing and treating reed wormwood artemisia disease it is usually used be chemical pesticide bactericide, due to a large amount of using chemical pesticides
The problem of environmental pollution caused is on the rise.Meanwhile, using chemical pesticide bactericide, plant pathogen strain is produced resistance
Property, the resistance to the action of a drug, add the difficulty of preventing and treating, or even cause preventing and treating to fail.In addition, chemical pesticide is because it is without selectivity, it is right
Beneficial biology will also result in threat.Moreover, many chemical pesticides are non-degradables or are difficult to degraded, easily in animal and plant body
Interior accumulation and pollute the enrichment of thing.
With requirement more and more higher of the public to " pollution-free food ", " pollution-free food ", market to it is pollution-free, can drop naturally
The ecological agricultural chemical demand of solution also gradually increases.From the point of view of the development of pesticide science, upper " back to nature " is used in pesticide manufacturing
And " Harmless " of agricultural chemicals, it is the inexorable trend of society and Development of Natural Science, therefore targetedly bactericide has extensively
Wealthy development prospect.
The content of the invention
Goal of the invention：It is an object of the invention to provide the good preventing and treating reed wormwood artemisia powdery mildew of a kind of environmental protection, bactericidal effect
Natural bactericidal agent.
Technical scheme：A kind of natural bactericidal agent for preventing and treating reed wormwood artemisia powdery mildew that the present invention is provided, is made up of following component：Greatly
Garlic, bitter dish, lotus leaf, Lappula echinata, lantana, Flos Magnoliae Liliflorae, sparrow Soviet Union, mantis egg-case, a red-spotted lizard Ba Zi, Rabdosia rubescens.
As an improvement, being made up of the component of following parts by weight：28-36 parts of garlic, 18-24 parts of bitter dish, 12-18 parts of lotus leaf,
8-12 parts of Lappula echinata, 6-10 parts of lantana, 6-10 parts of Flos Magnoliae Liliflorae, 4-8 parts of sparrow Soviet Union, 2-6 parts of mantis egg-case, 1-4 parts of a red-spotted lizard Ba Zi, Rabdosia rubescens
1-3 parts.
Improve, be made up of the component of following parts by weight as another：31-32 parts of garlic, 21-22 parts of bitter dish, lotus leaf 13-
14 parts, 9-10 parts of Lappula echinata, 8-9 parts of lantana, 7-8 parts of Flos Magnoliae Liliflorae, sparrow revive 6-7 parts, 4-5 parts of mantis egg-case, 2-3 parts of a red-spotted lizard Ba Zi, the winter
Insult 1-2 parts of grass.
Improve, be made up of the component of following parts by weight as another：31.8 parts of garlic, 21.4 parts of bitter dish, lotus leaf 13.3
Part, 9.7 parts of Lappula echinata, 8.2 parts of lantana, 7.6 parts of Flos Magnoliae Liliflorae, 6.5 parts of sparrow Soviet Union, 4.1 parts of mantis egg-case, 2.8 parts of a red-spotted lizard Ba Zi, Rabdosia rubescens
1.3 part.
Present invention also offers the preparation method of the natural bactericidal agent of above-mentioned preventing and treating reed wormwood artemisia powdery mildew, comprise the following steps：
(1) mixed after raw material being distinguished into size-reduced, sieving, obtain compound；The raw material is garlic, bitter dish, lotus leaf, crane
Lice, lantana, Flos Magnoliae Liliflorae, sparrow Soviet Union, mantis egg-case, a red-spotted lizard Ba Zi and Rabdosia rubescens；
(2) add mixture into 5-10 times measure 95% ethanol water, be heated to 50-70 DEG C decoction 2-4h；
(3) filter, filtrate is collected, 95% ethanol water is measured in 5-10 times of filter residue addition, be heated to 50-70 DEG C of decoction 2-
4h；
(4) filter, merging filtrate, be concentrated into the 1/20-1/10 that ethanol adds total amount, add 1-3 times of volume of water, stand
24-48h；
(5) filter, filtrate is concentrated into no alcohol taste, add with weight water dissolving, produce the natural of preventing and treating reed wormwood artemisia powdery mildew and kill
Microbial inoculum.
Present invention also offers the application of the natural bactericidal agent of above-mentioned preventing and treating reed wormwood artemisia powdery mildew, it is specially：Reed wormwood artemisia will be prevented and treated
The natural bactericidal agent of powdery mildew is measured water with 500-1000 times and mixed Ji Wei sterilization microbial inoculum.
Beneficial effect：The natural bactericidal agent raw material sources for the preventing and treating reed wormwood artemisia powdery mildew that the present invention is provided enrich, with low cost,
It is made of pure natural component, not only Nonpoisonous, non-environmental-pollution, and can effectively prevents and treats reed wormwood artemisia powdery mildew.
Embodiment
According to following embodiments, the present invention may be better understood.However, as it will be easily appreciated by one skilled in the art that real
Apply specific material proportion, process conditions and its result described by example and be merely to illustrate the present invention, without that will not also should limit
The present invention described in detail in claims processed.
Embodiment 1
The natural bactericidal agent of reed wormwood artemisia powdery mildew is prevented and treated, is made up of the component of following parts by weight：28 parts of garlic, 24 parts of bitter dish,
12 parts of lotus leaf, 12 parts of Lappula echinata, 6 parts of lantana, 10 parts of Flos Magnoliae Liliflorae, 4 parts of sparrow Soviet Union, 6 parts of mantis egg-case, 1 part of a red-spotted lizard Ba Zi, 3 parts of Rabdosia rubescens.
Its preparation method, comprises the following steps：
(1) mixed after raw material being distinguished into size-reduced, sieving, obtain compound；The raw material is garlic, bitter dish, lotus leaf, crane
Lice, lantana, Flos Magnoliae Liliflorae, sparrow Soviet Union, mantis egg-case, a red-spotted lizard Ba Zi and Rabdosia rubescens；
(2) ethanol water of 8 times of amounts 95% is added mixture into, 60 DEG C of decoction 3h are heated to；
(3) filter, filtrate is collected, filter residue adds the ethanol water of 8 times of amounts 95%, be heated to 60 DEG C of decoction 2-4h；
(4) filter, merging filtrate, be concentrated into ethanol adds total amount 1/15, add the water of 2 times of volumes of concentrate, stand
36h；
(5) filter, filtrate is concentrated into no alcohol taste, add with weight water dissolving, produce the natural of preventing and treating reed wormwood artemisia powdery mildew and kill
Microbial inoculum.
Embodiment 2
The natural bactericidal agent of reed wormwood artemisia powdery mildew is prevented and treated, is made up of the component of following parts by weight：36 parts of garlic, 18 parts of bitter dish,
18 parts of lotus leaf, 8 parts of Lappula echinata, 10 parts of lantana, 6 parts of Flos Magnoliae Liliflorae, 8 parts of sparrow Soviet Union, 2 parts of mantis egg-case, 4 parts of a red-spotted lizard Ba Zi, 1 part of Rabdosia rubescens.
Its preparation method, comprises the following steps：
(1) mixed after raw material being distinguished into size-reduced, sieving, obtain compound；The raw material is garlic, bitter dish, lotus leaf, crane
Lice, lantana, Flos Magnoliae Liliflorae, sparrow Soviet Union, mantis egg-case, a red-spotted lizard Ba Zi and Rabdosia rubescens；
(2) ethanol water of 5 times of amounts 95% is added mixture into, 70 DEG C of decoction 4h are heated to；
(3) filter, filtrate is collected, filter residue adds the ethanol water of 5 times of amounts 95%, be heated to 70 DEG C of decoction 4h；
(4) filter, merging filtrate, be concentrated into ethanol adds total amount 1/20, add the water of 1 times of volume of concentrate, stand
48h；
(5) filter, filtrate is concentrated into no alcohol taste, add with weight water dissolving, produce the natural of preventing and treating reed wormwood artemisia powdery mildew and kill
Microbial inoculum.
Embodiment 3
The natural bactericidal agent of reed wormwood artemisia powdery mildew is prevented and treated, is made up of the component of following parts by weight：32 parts of garlic, 22 parts of bitter dish,
14 parts of lotus leaf, 10 parts of Lappula echinata, 8 parts of lantana, 7 parts of Flos Magnoliae Liliflorae, 7 parts of sparrow Soviet Union, 4 parts of mantis egg-case, 3 parts of a red-spotted lizard Ba Zi, 2 parts of Rabdosia rubescens.
Its preparation method, comprises the following steps：
(1) mixed after raw material being distinguished into size-reduced, sieving, obtain compound；The raw material is garlic, bitter dish, lotus leaf, crane
Lice, lantana, Flos Magnoliae Liliflorae, sparrow Soviet Union, mantis egg-case, a red-spotted lizard Ba Zi and Rabdosia rubescens；
(2) ethanol water of 10 times of amounts 95% is added mixture into, 50 DEG C of decoction 2h are heated to；
(3) filter, filtrate is collected, filter residue adds the ethanol water of 10 times of amounts 95%, be heated to 50 DEG C of decoction 2h；
(4) filter, merging filtrate, be concentrated into ethanol adds total amount 1/10, add the water of 3 times of volumes of concentrate, stand
24h；
(5) filter, filtrate is concentrated into no alcohol taste, add with weight water dissolving, produce the natural of preventing and treating reed wormwood artemisia powdery mildew and kill
Microbial inoculum.
Embodiment 4
The natural bactericidal agent of reed wormwood artemisia powdery mildew is prevented and treated, is made up of the component of following parts by weight：31 parts of garlic, 21 parts of bitter dish,
13 parts of lotus leaf, 9 parts of Lappula echinata, 9 parts of lantana, 8 parts of Flos Magnoliae Liliflorae, 6 parts of sparrow Soviet Union, 5 parts of mantis egg-case, 2 parts of a red-spotted lizard Ba Zi, 1 part of Rabdosia rubescens.
Its preparation method is same as Example 1.
Embodiment 5
The natural bactericidal agent of reed wormwood artemisia powdery mildew is prevented and treated, is made up of the component of following parts by weight：31.8 parts of garlic, bitter dish 21.4
Part, 13.3 parts of lotus leaf, 9.7 parts of Lappula echinata, 8.2 parts of lantana, 7.6 parts of Flos Magnoliae Liliflorae, 6.5 parts of sparrow Soviet Union, 4.1 parts of mantis egg-case, a red-spotted lizard Ba Zi
2.8 parts, 1.3 parts of Rabdosia rubescens.
Its preparation method is same as Example 1.
Determine inhibitory activity of the natural bactericidal agent for preventing and treating reed wormwood artemisia powdery mildew to pathogen of embodiment 1 to 5
Comparative example is the microbial inoculum prepared using the method for embodiment 5, and consumption is identical with component weight in embodiment 5.
This experiment determines the natural bactericidal agent of preventing and treating reed wormwood artemisia powdery mildew of embodiment 1 to 5 to disease in vitro
The inhibitory activity of opportunistic pathogen, method and result are as follows：
1. for examination strain
White cross hair list softgel shell Podosphaera leucotricha are commercially available.
2. Toxicity Determination
The natural bactericidal agent dialogue cross hair list capsule of the embodiment of the present invention 1 is determined using mycelial growth rate method and spore germination method
Shell Podosphaera leucotricha virulence, inhibiting rate is calculated by following equation：
3. experimental result
Embodiment 1-5 natural bactericidal agents are determined to the virulence for trying pathogen with growth rate method, the results are shown in Table 1.
The embodiment 1-5 natural bactericidal agents of table 1 are to the virulence for trying pathogen
Using《Pesticide field efficacy medicine test criterion》Specified in method, to the natural bactericidal agent of the embodiment of the present invention 1 to 5
Field efficacy tested, diluted concentration be 500-1000 times, the results are shown in Table 2.
To for examination pathogen：White cross hair list softgel shell Podosphaera leucotricha.
The field control effectiveness test of the natural bactericidal agent of the embodiment 1 to 5 of table 2
Reed wormwood artemisia powdery mildew, respectively 100 plants of the treatment, extension rate of various diseases are treated using the natural bactericidal agent of embodiment 1 to 5
For 500 times, 3 are the results are shown in Table.
To for examination pathogen：White cross hair list softgel shell Podosphaera leucotricha.
The natural bactericidal agent treatment reed wormwood artemisia powdery mildew of the embodiment 1 to 3 of table 3
Claims (5)
1. a kind of preparation method for the natural bactericidal agent for preventing and treating reed wormwood artemisia powdery mildew, it is characterised in that：Comprise the following steps：
(1) mixed after raw material being distinguished into size-reduced, sieving, obtain compound；The raw material is garlic, bitter dish, lotus leaf, Lappula echinata, horse
Tassel flower, Flos Magnoliae Liliflorae, sparrow Soviet Union, mantis egg-case, a red-spotted lizard Ba Zi and Rabdosia rubescens；
(2) add mixture into 5-10 times measure 95% ethanol water, be heated to 50-70 DEG C decoction 2-4h；
(3) filter, filtrate is collected, 95% ethanol water is measured in 5-10 times of filter residue addition, be heated to 50-70 DEG C of decoction 2-4h；
(4) filter, merging filtrate, be concentrated into the 1/20-1/10 that ethanol adds total amount, add 1-3 times of volume of water, stand 24-
48h；
(5) filter, filtrate is concentrated into no alcohol taste, add with weight water dissolving, produce the natural bactericidal agent of preventing and treating reed wormwood artemisia powdery mildew.
2. a kind of preparation method of natural bactericidal agent for preventing and treating reed wormwood artemisia powdery mildew according to claim 1, it is characterised in that：
It is made up of following component：Garlic, bitter dish, lotus leaf, Lappula echinata, lantana, Flos Magnoliae Liliflorae, sparrow Soviet Union, mantis egg-case, a red-spotted lizard Ba Zi, Rabdosia rubescens.
3. a kind of preparation method of natural bactericidal agent for preventing and treating reed wormwood artemisia powdery mildew according to claim 1, it is characterised in that：
It is made up of the component of following parts by weight：28-36 parts of garlic, 18-24 parts of bitter dish, 12-18 parts of lotus leaf, 8-12 parts of Lappula echinata, lantana
6-10 parts, 6-10 parts of Flos Magnoliae Liliflorae, sparrow revive 4-8 parts, 2-6 parts of mantis egg-case, 1-4 parts of a red-spotted lizard Ba Zi, 1-3 parts of Rabdosia rubescens.
4. a kind of preparation method of natural bactericidal agent for preventing and treating reed wormwood artemisia powdery mildew according to claim 1, it is characterised in that：
It is made up of the component of following parts by weight：31-32 parts of garlic, 21-22 parts of bitter dish, 13-14 parts of lotus leaf, 9-10 parts of Lappula echinata, lantana
8-9 parts, 7-8 parts of Flos Magnoliae Liliflorae, sparrow revive 6-7 parts, 4-5 parts of mantis egg-case, 2-3 parts of a red-spotted lizard Ba Zi, 1-2 parts of Rabdosia rubescens.
5. a kind of preparation method of natural bactericidal agent for preventing and treating reed wormwood artemisia powdery mildew according to claim 1, it is characterised in that：
It is made up of the component of following parts by weight：31.8 parts of garlic, 21.4 parts of bitter dish, 13.3 parts of lotus leaf, 9.7 parts of Lappula echinata, lantana 8.2
Part, 7.6 parts of Flos Magnoliae Liliflorae, 6.5 parts of sparrow Soviet Union, 4.1 parts of mantis egg-case, 2.8 parts of a red-spotted lizard Ba Zi, 1.3 parts of Rabdosia rubescens.
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