CN1070232C - Method for preparing cepaeo enzyme - Google Patents
Method for preparing cepaeo enzyme Download PDFInfo
- Publication number
- CN1070232C CN1070232C CN98121947A CN98121947A CN1070232C CN 1070232 C CN1070232 C CN 1070232C CN 98121947 A CN98121947 A CN 98121947A CN 98121947 A CN98121947 A CN 98121947A CN 1070232 C CN1070232 C CN 1070232C
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- China
- Prior art keywords
- helicase
- snail
- leftovers
- preparation
- damping fluid
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- Meat, Egg Or Seafood Products (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present invention relates to method for recovering and producing a helicase from leftovers generated after snail meat is produced from a raw material snail. At present, the snail leftovers are discarded, which wastes resources and pollutes the environment; even the leftovers are directly dried to be used as feedstuff, the utilization rate is very low. After treating the snail leftovers, the method uses a buffer solution to extract the helicase; then, the helicase is made into dried frozen products by sterilization and drying; the feedstuff is processed, etc. The method greatly increases the utilization rate of the snail leftovers and obtains good social benefit and economic benefit.
Description
The present invention is a kind of method of extracting recovery helicase preparation from the discarded internal organs of animal.
Since the snail artificial breeding for people as delicious food after, tankage such as the internal organ of snail are utilized always, for a long time the tankage of snail abandon need not, both wasted resource, polluted environment again.Though also useful snail tankage oven dry used as fodder, utilization ratio is too low, brings into play its due effect far away, and is very unfortunate.How to bring into play the nutritive value of its tankage of snail of being rich in nutrition value, it is made the best use of everything, getting twice the result with half the effort is the target that people seek always.
The objective of the invention is to invent the method for the extraction helicase that a kind of technology is simple, utilization ratio is high, the method that the present invention produces helicase is as follows:
(1) snail is fetched to starve and supported 2 ∽ 5 days, drains food in the body as far as possible, washes most snail body surface mud and movement;
(2) grind with easy device and loose or strike diffusing shell, separate housings is taken out edible Carnis Limax;
(3) collect stomach, crop and liver part in the tankage, put into the glass cylinder that is chilled to 8 ℃ of 2 ∽ in advance;
(4) rub internal organ such as stomach with high speed homogenizer or mincer;
(5) in 8 ℃ of refrigerators of 2 ∽, stir 2～3 hours extraction helicases with damping fluids such as acetate, phosphoric acid salt, tris;
(6) in the whizzer in the said temperature scope with 8000～12000 rev/mins, centrifugal 20～30 minutes, supernatant liquor was put in 4 ℃ of refrigerators and preserves.In order fully to extract helicase, the precipitation part is again with the damping fluid of one times of internal organ slurry volume, and the same method repeats once.
(7) merge two times centrifugal liquid, with 0.45 Ц strainer filtration sterilization.The precipitation part can be made feed and use;
(8) bacteria-removing liquid is in freezing two days of-30 ℃ of refrigerators, changes over to then in-30 ℃ the lyophilizer and makes dried frozen aquatic products;
(9) suitably mill is diffusing for dried frozen aquatic products, and packing is on demand preserved at-18 ℃ of refrigerators.
Among the preparation method of the present invention, the extracting solution of enzyme is a damping fluid, but with NaH
2PO
4H
2O and NaHPO
42H
2O is for well, and extraction effect is good, and convenient post-treatment is more easy to operate.
For well, it is not high that damping fluid has lacked extraction effect with 4 times of 1 ∽ of internal organ slurry volume for the consumption of above-mentioned damping fluid, many causes waste, and postchannel process is wasted time and energy, and increased preparation cost.
Damping fluid NaH
2PO
4H
2O and Na
2HPO
42H
2The concentration of O is respectively 20.0-26.0g/L and 2.00-3.00g/L, and all the other are water.
The present invention is 8000-12000 rev/min with the centrifugal speed of whizzer, can effectively separate enzyme liquid and impurity after centrifugal.
The inventive method is easy, easy suitability for industrialized production, and agents useful for same and equipment are conventional article, are that enterprise accepts easily to setting up suitability for industrialized production.The helicase that the inventive method makes after tested, the result shows that it is active basic identical with the standard helicase, and amylase wherein, cellulase and polygalacturonase are all keeping quite high activity, with 8 yuan of calculating of one kilogram of snail, then the helicase made of the tankage that stay of one kilogram of snail can get about 60 yuan.Therefore present method is after extracting helicase, carries out feed processing again, has not only cleaned environment, has also improved the effective rate of utilization of snail tankage greatly, obtains good social benefit and economic benefit.
The measuring method of helicase of the present invention is as follows:
Adopt the microscopic counting method of yeast cell to measure the active relative value of helicase.
Get 1.5 milliliters of yeast nutrient solutions, centrifugal collection thalline is made broken wall treatment with a certain amount of standard helicase, and wherein half goes up growth at regeneration culture medium (A), and second half goes up growth at non-regeneration culture medium (B), and the difference of yeast bacterium colony is the broken wall number.Measure under the same conditions with same amount self-control helicase, calculate the broken wall number equally.Broken wall number to both is made comparisons, and is standard with the broken wall number of standard helicase, calculates the relative reactivity of self-control helicase.Each tests triplicate.As experimental result once be: the broken wall number of standard helicase is (83,97,89)/ware, and is average 89.7, and the helicase broken wall number that oneself extracts is (92,86,94)/ware, average 90.7.The result shows that its activity of homemade helicase is basic identical with the standard helicase.
Embodiment: (is example with the Laboratory Production helicase)
(1) snail was fetched the back hungry foster 2 days, drained food in the body as far as possible, washed most snail body surface mud and movement.
(2) strike diffusing shell, and separate housings, edible Carnis Limax taken out.
(3) stomach, the crop part in the collection tankage put into the glass cylinder of precooling.
(4) with internal organ such as high speed homogenizer homogenate stomaches.
(5) with the long-pending damping fluid of the even liquid of twice internal organ, in 4 ℃ of refrigerators, stir and extracted helicase in 3 hours.
(6) in 4 ℃ of whizzers with 10000 rev/mins, centrifugal 25 minutes, supernatant liquor was put in 4 ℃ of refrigerators and preserves.The precipitation part is again with the damping fluid of 1 times of internal organ homogenate volume, and the same method repeats once again.
(7) merge two times centrifugal liquid, with 0.45 Ц strainer filtration sterilization.The precipitation part can be made feed and use.
(8) bacteria-removing liquid-30 ℃ freezing two days, change over to then in-30 ℃ the lyophilizer and make dried frozen aquatic products.
(9) suitably mill is diffusing for dried frozen aquatic products, and packing is on demand preserved at-18 ℃ of refrigerators.
Claims (5)
1. the preparation method of a helicase is characterized in that:
(1) before helicase extracted, snail was starved and supported 2 ∽ 5 days;
(2) taking out behind the edible Carnis Limax tankage, to put 8 ℃ of low temperature of 2 ∽ under pulverizing stand-by;
(3) stir under the said temperature scope with damping fluid;
(4) centrifugal under said temperature;
(5) filtration sterilization, lyophilize.
2. the preparation method of helicase according to claim 1 is characterized in that its damping fluid is NaH
2PO
4H
2O, NaHPO
42H
2O.
3. the preparation method of helicase according to claim 1, the consumption that it is characterized in that damping fluid is 4 times of 1 ∽ of internal organ slurry volume.
4. the preparation method of helicase according to claim 1 is characterized in that damping fluid NaH
2PO
4H
2O and NaPO
42H
2The concentration of O is respectively 20.0-26.0g/L and 2.00-3.00g/L, and all the other are water.
5. the preparation method of helicase according to claim 1, the centrifugal speed that it is characterized in that whizzer is 12000 rev/mins of 8000 ∽.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98121947A CN1070232C (en) | 1998-10-10 | 1998-10-10 | Method for preparing cepaeo enzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98121947A CN1070232C (en) | 1998-10-10 | 1998-10-10 | Method for preparing cepaeo enzyme |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1216778A CN1216778A (en) | 1999-05-19 |
| CN1070232C true CN1070232C (en) | 2001-08-29 |
Family
ID=5227438
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98121947A Expired - Fee Related CN1070232C (en) | 1998-10-10 | 1998-10-10 | Method for preparing cepaeo enzyme |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1070232C (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102199582B (en) * | 2010-03-23 | 2013-04-10 | 叶阿彬 | Method for extraction and preservation of helicase |
| CN103468663A (en) * | 2013-08-12 | 2013-12-25 | 叶阿彬 | Snail protease extraction method |
| CN104642712B (en) * | 2015-02-16 | 2018-06-19 | 威海瑞佳丽生物科技有限公司 | A kind of extracting method of Ovum Limax white matter powder |
| CN104586732B (en) * | 2015-02-16 | 2018-03-06 | 威海瑞佳丽生物科技有限公司 | A kind of method of the snail pituitary extract extraction of active enzyme |
| CN104560931B (en) * | 2015-02-16 | 2018-03-06 | 威海瑞佳丽生物科技有限公司 | A kind of extracting method of glusulase |
| CN104726440A (en) * | 2015-04-20 | 2015-06-24 | 威海圣奥罗生物科技有限公司 | Snail enzyme preparation method |
| CN106011127A (en) * | 2016-06-25 | 2016-10-12 | 陈毅忠 | Method for preparing chitosan oligosaccharide by using immobilized snailase |
| CN106086118A (en) * | 2016-06-25 | 2016-11-09 | 仇颖超 | A kind of method that Limax resource prepares chitin oligosaccharide |
| CN108244339A (en) * | 2017-12-29 | 2018-07-06 | 常州市阿曼特化工有限公司 | A kind of preparation method rich in antimicrobial peptide protein powder feed additive |
| CN110272886A (en) * | 2019-05-30 | 2019-09-24 | 重庆多拉吧生物科技有限公司 | A kind of preparation method of snail enzyme solution and products thereof and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1083353A (en) * | 1993-07-20 | 1994-03-09 | 乌力吉 | Full snail skin-care nutrient liquid and preparation method thereof |
| CN1086424A (en) * | 1993-07-19 | 1994-05-11 | 乌力吉 | Limax cattle nutrition extracting solution and preparation method thereof |
| CN1092645A (en) * | 1993-03-16 | 1994-09-28 | 刘春增 | The skin care item and the manufacture method that contain snail extract |
| CN1099415A (en) * | 1994-07-02 | 1995-03-01 | 张华玲 | Snail bioenzyme and snail biotin |
-
1998
- 1998-10-10 CN CN98121947A patent/CN1070232C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1092645A (en) * | 1993-03-16 | 1994-09-28 | 刘春增 | The skin care item and the manufacture method that contain snail extract |
| CN1086424A (en) * | 1993-07-19 | 1994-05-11 | 乌力吉 | Limax cattle nutrition extracting solution and preparation method thereof |
| CN1083353A (en) * | 1993-07-20 | 1994-03-09 | 乌力吉 | Full snail skin-care nutrient liquid and preparation method thereof |
| CN1099415A (en) * | 1994-07-02 | 1995-03-01 | 张华玲 | Snail bioenzyme and snail biotin |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1216778A (en) | 1999-05-19 |
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