CN1086424A - Limax cattle nutrition extracting solution and preparation method thereof - Google Patents
Limax cattle nutrition extracting solution and preparation method thereof Download PDFInfo
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- CN1086424A CN1086424A CN93108656A CN93108656A CN1086424A CN 1086424 A CN1086424 A CN 1086424A CN 93108656 A CN93108656 A CN 93108656A CN 93108656 A CN93108656 A CN 93108656A CN 1086424 A CN1086424 A CN 1086424A
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Abstract
The present invention has kept content and bioactive whole snail nutrient liquor to human body effective ingredient and Snailase by the Limax of rich in protein and internal organs extracting solution thereof through make aseptic of processes such as Hollow Fiber Ultrafiltration degerming.Can replenish the nutrient of needed by human body effectively, cell activity enhancing enhances metabolism; Wherein mucin still can exclude free radical, and uvioresistant has crease-resistant, sun-proof, anti-aging effect; With and the closely relation very close with human body cell, its nutritional labeling is beneficial to absorption of human body, in fields such as cosmetics such as beauty treatment, skin protection, hair washing, hair care and medicines and health protections, has a extensive future.
Description
The present invention relates to the animal extracts and the extracting method thereof of cosmetic field, the application of medicines and health protection field.
Mucin of Limax and protein content shelter have first of the animal, include abundant special composition: the natural nutrition composition of diversification such as mucin (glucoprotein), 18 kinds of human bodies can't synthetic essential amino acids, the trace element of the enzyme of fatty acid, organic acid, vitamin, more than 30 kind of biologically active and immunocompetence thing, several mineral materials and needed by human body, selenium, zinc, boron, calcium, phosphorus, ferrum.Therefore it is the nutritious traditional food of a kind of high protein, low fat and has important medical value, successfully from the digestive gland of Limax, separate, extracted Snailase, from the intravital albumen body of gland of snail, extract the agglutinin that hematology's research is had using value.Limax is applied to other fields widely, is more and more paid attention to by people; As cosmetic field, medical health field etc., key is how to extract its effective nutrition from Limax.Extracting method must be that technology is easy, is applicable to enlarge to produce.People are provided receptible reasonable price, stable and aseptic snail nutrient liquor, at present still the end appear in the newspapers to.
Purpose of the present invention is by strict feasible extraction process operating procedure; adopt new degerming method; can filter the whole antibacterials in the clean snail extract fully; and protected the bioactive substance of whole active ingredients of human body and Limax is not suffered a loss and destroyed, thereby provide a kind of stable and aseptic snail nourishing extracting solution.
Limax cattle nutrition extracting solution of the present invention is with the meat of selected Limax alive and 100 parts of the extracting solution (weight) of internal organs thereof, vitamin C 3～5 parts of (weight) and 0.1 part of (weight) composition of antisepsis and sterilization, and realize by the polysulfone hollow fibre ultrafiltration apparatus heat sterilization of 8～150,000 molecular weight.Polysulfone hollow fibre with 100,000 molecular weight is best.The degerming rate can reach 100%.Can filter whole antibacterials of clean snail extract fully, and protect the biological activity in the snail nourishing extracting solution all effective composition filtration of human body and Snailase.
Snail nutrient liquor manufacturing process steps of the present invention is as follows:
(1) selected Limax
. every artificial breeding's Limax all can be selected for use,
. the requirement nothing is ill, active, body is strong, healthy,
. every weight can be selected for use more than 10 grams, and all between 10 grams-80 grams, maximum weight can reach 500 grams to general every the weight of domestic artificial breeding's Limax, and is better with every 30-60g,
. can not select frowziness for use, shell and dead Limax contract.
. put into the foraminate plastic box in bottom as the Limax of extracting usefulness and rest selected, every case keeps flat at most into one deck to well, can not pile pressure.
. the Limax that will live rested in hungry 48-72 hour, no longer fed and let alone what beverage, and Limax has drained food and the feces in the clean the intestines and stomach substantially after resting.
. every 12 hours with about 15 °～25 ℃ flushing with clean water Limax once, the time sooner or later of preferably selecting for use is washed away the Excreta (but must not water is single do the wash, in case minimizing body fluid) of Limax.After the flushing, both avoided Limax to eat up excretory feces again, also played the moisture content that maintenance and additional Limax should need self because of hunger.
(2) slaughtering and the separation of meat, internal organs of Limax:
. the Limax that rests is rinsed well with water purification, reuse bleaching powder water or the flushing of nontoxic antibacterial one time, to go out except that the antibacterial of Limax surface, quiet again putting 4 hours after pulling out treats can slaughter after calcium hypochlorite (bleaching powder) vapors away fully,
. Limax is placed on the operating board, Crusta Limax is broken into pieces, note not striking bad meat and internal organs, firmly want evenly when striking with the lumps of wood,
. peel off broken shell (doing it in addition after drying, the pulverizing uses), catch Carnis Limax with hands, internal organs cut (attention: slightly leave internal organs some distance along Limax with the internal organs marginal portion with the rustless steel shears, in case break internal organs, Snailase is flowed out, cause damage), complete meat is separated also placed apart with internal organs.
(3) operating process of Carnis Limax and internal organs extracting solution:
(3.1), meat is put into bottom band valve and had the container of agitating device (available rustless steel or enamelled any) by Carnis Limax: water is 1: 1～3(weight portion), adding deionized water stirs, mixing speed is 150～180 rev/mins, with 180 rev/mins better, whenever stirred 2 minutes, left standstill 3 minutes, and repeated 5 times.Then, put that clean to dissolve the whole liquid that standby; Reform 2 times by above method and step, will become the Limax of lump to blend the 4th time, using the same method adds water, repeats once to emit dissolved solution for later use again.It is standby to collect 4 extracting solution.(do its usefulness after the drying in addition, annotate: if each 10 kilograms of Carnis Limaxs of processing, the total amount of water is no less than 20 kilograms, must not be more than 80 kilograms to take out remaining dregs; Amount to extracting solution and must not be less than 22.5 kilograms, must not be more than 82.5 kilograms).
(3.2), as method as described in (3.1) earlier with whole internal organs of Limax by internal organs: water be 1: 1～3 parts (weight) add deionized water first molten after, take out dissolved solution for later use.
(3.2.1) for the second time the whole internal organs of Limax are blended, add 2 parts of (weight) water, stirring and dissolving, collect lysate.
(3.2.2), stir after 5 minutes, filter, get and filter the back lysate with 100 order nylon sieves for the third time with the water of remaining internal organs addings 1～4 part (weight),
(3.2.3) the 4th time with remaining material by adding 2 parts of (weight) water again, after the stirring and dissolving, it is standby to get the extracting solution that filters out.Outwell dregs (doing it after the drying in addition uses) after having filtered.It is standby to collect 4 Limax internal organs extracting solution altogether.Extract the weight of described every part of water and the first time being equal in weight of the whole internal organs of Limax.
Amount to the adding water yield and must not be less than 20 kilograms for per 10 kilograms, must not amount to extracting solution and must not be less than 23.5 kilograms more than 80 kilograms, must not be more than 89.5 kilograms.
Described water can be any in deionized water or distilled water or the mineral water.
The extracting solution of above Limax and internal organs is respectively the filtration mechanical filter of 2-10 micron (best with 5 microns with the aperture) with the aperture; load in mixture together; the anticorrosion and bactericidal agent that adds 0.1 part (weight) with per 100 parts of (weight) extracting solution; vitamin C (the ascorbic acid that adds 3-5 part (weight) again; very strong antioxidation and reduction are arranged; can resist the Oxidation of the external world and inner tool oxidizing substance, protection wherein Snailase and other easy inactivation and oxidation the activity of effective constituent).After the dissolving, stir, leave standstill, make it abundant hydrolysis (also can carry out composite at this moment).
Put into storage tank, pass through again arranged side by side 2 100,000 or 8-15 ten thousand molecular weight with the hollow-fibre ultrafiltration device ultrafiltration of the best polysulfones of 100,000 molecular weight 2 times after, the whole antibacterials that have in filter is clean.The bacteriological filtration rate is 99.95% and＞99.98% for the first time, and the degerming rate is 100% after the ultrafiltration second time.
Promptly get through-5 ℃ the freezing 8-12 of freezing equipment hour again and stablize and aseptic Limax cattle nutrition extracting solution, or the whole snail nutrient liquor before the various fill.Can place in 1-5 ℃ the low-temperature (low temperature) vessel and preserve.
The snail nourishing extracting solution of making by the inventive method is because aseptic and stably kept content * and the biological activity * * to effective nutrition of human body (table 1, table 2) and the Snailase that contain in the former Limax,
Therefore:
1, have superior bioactive and rapid osmotic effect, benefit the characteristics of partes corporis humani position fast Absorption, can replenish the nutrient of needed by human body effectively, cell activity enhancing can improve the required mechanism of inherent nutrient of human body effectively, all sidedly, enhance metabolism oral administration or external.
2, the contained special composition-mucin of Limax except that having general nutritve function, still can exclude free radical, can resist ultraviolet after being applied in skin surface, has effect significantly crease-resistant, sun-proof, that prevent skin aging.
3, based on the diversification and the practical value of Limax idiotrophic composition, and the sibship very close with human body cell, its contained nutrition all is beneficial to the fast Absorption of human body, and its range of application to the mankind is had a extensive future.As be used to improve looks, the production of high-grade natural prodcuts such as skin protection, hair washing, hair care, bath lotion; Also can be used for preparing high-grade nutrition official ceremonial dress liquid, and make all kinds of health product, health care oral liquid, concentrated solution etc.
Example 1
Select 10 kilograms of the healthy active Limaxs of every weight 40～50 grams for use, after hunger was rested 60 hours, with 20 ℃ of left and right sides flushing with clean water and with after Eusol flushing one time, placing 4 hours, with Limax on operating board, with the lumps of wood shell is broken into pieces, peel off broken shell, internal organs are cut with the internal organs marginal portion along Limax with the rustless steel shears, meat and internal organs is placed apart.The rustless steel container of isolating 3.5 kilograms of Carnis Limaxs being put into bottom band valve and belt stirrer adds 7 kilograms of deionized waters, stirs and trembles 2 minutes, and 180 rev/mins of mixing speeds left standstill 3 minutes, so repeated 5 times.Then, whole liquid of putting after the clean dissolving are standby; Reform 2 times by above method step again, will become the Limax of lump to blend the 4th time, use with quadrat method to add water, emit dissolved solution for later use.It is standby to collect 27.8 kilograms of extracting solution of 4 dissolvings of Carnis Limax altogether.The deionized water of 2.5 kilograms in isolated Limax internal organs is soaked, stirs, dissolves the back for the first time take out dissolved solution for later use.For the second time, it is blended into the meat stuffing shape with mechanical means again with whole internal organs (comprising ovum) of Limax, add the dissolving of 5 kg of water after, take out dissolved solution for later use; To remain for the third time and add 5 kilograms of deionized waters in the internal organs, fully stir 5 minutes after, filter with 100 order nylon mesh, filter back taking-up dissolved solution for later use.The 4th time remaining internal organs are added 5 kilograms deionized water again, the stirring and dissolving after-filtration is got filtrate for later use.Receive 19.2 kilograms of 4 internal organs extracting solution altogether.
After above-mentioned Carnis Limax and internal organs extracting solution filtered with the filter in 5 microns in aperture respectively, load in mixture together, add the edible anticorrosion and bactericidal agent of 0.047 gram, add 1.8 gram vitamin Cs again, after the dissolving, stirred, leave standstill 4 hours, and put into storage tank, filter by 2 100,000 molecular weight polysulfone hollow fibre defecators arranged side by side, the degerming rate reaches 100% after 2 ultrafiltration again.Promptly got in freezing 10 hours through-5 ℃ again and stablize and 48.2 kilograms of aseptic snail nourishing extracting solution.
Analysis of amino acids in table 1, the mucus (saline solution) (unit: mg/100g)
| Before the extraction | After the extraction | Before the extraction | After the extraction | ||
| Aspartic acid | 66.7 | 6.67 | Glycine | 41.4 | 3.6 |
| Methionine | 5.3 | 0.49 | Alanine | 27.7 | 2.57 |
| Threonine | 25.2 | 2.7 | Cystine | 6.9 | 5.8 |
| Isoleucine | 19.9 | 2.0 | Valine | 24.4 | 2.12 |
| Serine | 25.7 | 2.43 | Lysine | 24.6 | 2.20 |
| Leucine | 38.4 | 3.69 | Histidine | 14.2 | 1.3 |
| Glutamic acid | 110.6 | 12.1 | Ammonia | 7.8 | 0.61 |
| Phenylalanine | 21.1 | 2.03 | Arginine | 33.8 | 3.25 |
Analysis of amino acids in table 2, the Carnis Limax (831-50AA automatic amino acid analyzer) (unit: %)
| Before the extraction | After the extraction | Before the extraction | After the extraction | ||
| Aspartic Acid | 5.13 | 0.63 | Isoleucine | 2.18 | 1.12 |
| Threonine | 2.25 | 0.28 | Leucine | 3.85 | 0.57 |
| Serine | 2.77 | 0.41 | Tyrosine | 1.54 | 0.4 |
| Glutamic acid | 8.25 | 1.12 | Phenylalanine | 1.79 | 0.21 |
| Glycine | 4.29 | 0.5 | Lysine | 2.95 | 0.35 |
| Alanine | 2.87 | 0.36 | Ammonia | 0.86 | 0.017 |
| Cystine | 0.58 | 0.07 | Histidine | 0.82 | 0.01 |
| Valine | 2.17 | 0.26 | Arginine | 4.14 | 0.5 |
| Methionine | 0.30 | 0.11 | Proline | 2.21 | 0.26 |
* Snailase assay in the Limax cattle extracting solution:
The Limax body contains cellulase, hemicellulase, Lactose enzyme, saccharase, the mixed enzyme of more than 30 kind of biologically active such as protease, the Limax cattle extracting solution of having made is told 1000 grams (use 10000 rev/mins of refrigerated centrifuges through centrifugal, centrifugal 10 minutes, stay supernatant, discard precipitate)-→ leak head with No. four antibacterials to filter, collect filtrate, carry out after filtrate being pre-chilled to-20 ℃-→ vacuum drying after still tan dried Snailase 0.86 gram, if be converted into the Digestive system of equivalent in proportion, extract Snailase in the Digestive system, answer the dried Snailase of the clear 0.91 gram dried Snailase of gained in the ratio of the Limax cattle extracting solution of 62.5ml Limax Digestive system melt into 1000ml/ gram (promptly with).
The active mensuration of Snailase in the * Limax cattle extracting solution:
Through with phase contrast microscope observational method, nephelometry and protein determination etc.,, thereby learn that the activity of Snailase in the extracting solution and Limax digest the Snailase that extracts in the body of gland, no significant difference with the number of inspection spheroplast.
Claims (10)
1, a kind of Limax cattle nutrition extracting solution, it is characterized in that 100 parts of extracting solution (weight), the no bacterial nutrient solution that vitamin C 3-5 part (weight) and anticorrosion and bactericidal agent 0.1 part (weight) are formed and the doughnut filtration sterilization by 8-15 ten thousand molecular weight realizes with Carnis Limax and internal organs thereof.
2, Limax cattle nutrition extracting solution as claimed in claim 1 is characterized in that with the polysulfone hollow fibre filtration sterilization of 100,000 molecular weight best.
3, a kind of method of making the described Limax cattle of claim 1 nutrition extracting solution:
(1) selected Limax
(1.1) select that every weight is strengthened at 10 grams above active, body, healthy Limax alive for use,
(1.2) Limax that will live rested in hungry 48-72 hour, every 12 hours once with 15 °～25 ℃ flushing with clean water,
(2) Limax slaughter and meat, internal organs separate:
(2.1) again Limax is rinsed well with 15 °～25 ℃ clear water, with nontoxic antibacterial flushing one time,
(2.2) Crusta Limax is broken into pieces, peelled off broken shell, complete meat is separated also placed apart with internal organs.
(3) operating process of Carnis Limax and internal organs extracting solution:
(3.1), Limax is put into the container that band valve in bottom has agitating device, by Carnis Limax: water is 1: 1～3 parts (weight), and the adding deionized water stirs, mixing speed is 150～180 rev/mins and whenever stirred 2 minutes, left standstill 3 minutes, and repeated 5 times, the liquid of putting after the clean dissolving is standby; Again by above method and step retry 2 times, the 4th time Carnis Limax is blended, add water and repeat again 1 time, it is standby to collect 4 extracting solution,
(3.2), as (3.1) method step, with the internal organs of Limax by internal organs: water is that 1: 1～3 parts (weight) adds deionized water, after the dissolving, takes out dissolved solution for later use for the first time.
(3.2.1) the Limax internal organs are blended, add 2 parts of (weight) water, stirring and dissolving, collect lysate.
(3.2.2), stir after 5 minutes, filter, get filtrate with 100 order nylon sieves for the third time with the water of remaining internal organs addings 1～4 part (weight),
(3.2.3) add 2 parts of (weight) water for the 4th time again, stir, dissolve, filter, get filtrate, collect 4 times extracting solution,
The weight of described every part of water is equal in weight with the preceding whole internal organs of Limax of the extraction first time,
(4) with (3.1) Carnis Limax and (3.2) Limax internal organs extracting solution with the aperture be 2～10 microns filtration mechanical filter respectively, mix, adding anticorrosion and bactericidal agent and vitamin C,
(5) poly-sulfur doughnut filters 2 times,
(6) freezing through-5 ℃.
4, as claimed in claim 3ly prepare the method that the snail nutrient liquor dike is got liquid, every weight with 30～60 grams better in the snail that it is characterized in that (1.1) selecting for use.
5, the method for preparing snail nutrient liquor as claimed in claim 3, described non-toxic bactericide can be a bleaching powder.
6, the method for preparing snail nutrient liquor as claimed in claim 3 is characterized in that (3.1) described bottom band valve and the container that agitating device is arranged are any in rustless steel or the enamel.
7, the method for preparing snail nutrient liquor as claimed in claim 3 is characterized in that (3.1) Limax internal organs: water best 1: 2(weight) better.
8, the method for preparing snail nutrient liquor as claimed in claim 3 is characterized in that (3) described deionized water also can be any in distilled water or the mineral water.
9, the method for preparing snail nutrient liquor as claimed in claim 3 is characterized in that (4) are that 5 microns filtration mechanical filter is best with the aperture.
10, the method for preparing the snail nourishing extracting solution as claimed in claim 3, the mixing speed that it is characterized in that (3.1) with 180 rev/mins for well.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93108656A CN1086424A (en) | 1993-07-19 | 1993-07-19 | Limax cattle nutrition extracting solution and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93108656A CN1086424A (en) | 1993-07-19 | 1993-07-19 | Limax cattle nutrition extracting solution and preparation method thereof |
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| Publication Number | Publication Date |
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| CN1086424A true CN1086424A (en) | 1994-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN93108656A Pending CN1086424A (en) | 1993-07-19 | 1993-07-19 | Limax cattle nutrition extracting solution and preparation method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1070232C (en) * | 1998-10-10 | 2001-08-29 | 复旦大学 | Method for preparing cepaeo enzyme |
| CN1321587C (en) * | 2005-08-30 | 2007-06-20 | 宋兆华 | Snail health product and its preparation method |
| CN103468663A (en) * | 2013-08-12 | 2013-12-25 | 叶阿彬 | Snail protease extraction method |
| CN104642712A (en) * | 2015-02-16 | 2015-05-27 | 曾丽娟 | Extracting method for snail protein powder |
| CN105982838A (en) * | 2015-03-05 | 2016-10-05 | 何武盛 | Snailase repair stock solution and preparation method thereof |
| CN107475342A (en) * | 2017-10-17 | 2017-12-15 | 陈和平 | A kind of extracting method of snail active peptide |
| CN110314247A (en) * | 2019-08-02 | 2019-10-11 | 深圳国佳产业基金管理有限公司 | Snail essence extract active dressing and preparation method thereof |
| CN110420164A (en) * | 2019-09-29 | 2019-11-08 | 国佳凝胶科创中心(深圳)有限公司 | A kind of snail essence dry powder and the preparation method and application thereof |
-
1993
- 1993-07-19 CN CN93108656A patent/CN1086424A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1070232C (en) * | 1998-10-10 | 2001-08-29 | 复旦大学 | Method for preparing cepaeo enzyme |
| CN1321587C (en) * | 2005-08-30 | 2007-06-20 | 宋兆华 | Snail health product and its preparation method |
| CN103468663A (en) * | 2013-08-12 | 2013-12-25 | 叶阿彬 | Snail protease extraction method |
| CN104642712A (en) * | 2015-02-16 | 2015-05-27 | 曾丽娟 | Extracting method for snail protein powder |
| CN104642712B (en) * | 2015-02-16 | 2018-06-19 | 威海瑞佳丽生物科技有限公司 | A kind of extracting method of Ovum Limax white matter powder |
| CN105982838A (en) * | 2015-03-05 | 2016-10-05 | 何武盛 | Snailase repair stock solution and preparation method thereof |
| CN107475342A (en) * | 2017-10-17 | 2017-12-15 | 陈和平 | A kind of extracting method of snail active peptide |
| CN110314247A (en) * | 2019-08-02 | 2019-10-11 | 深圳国佳产业基金管理有限公司 | Snail essence extract active dressing and preparation method thereof |
| CN110314247B (en) * | 2019-08-02 | 2020-05-29 | 深圳国佳产业基金管理有限公司 | Snail essence extract active dressing and preparation method thereof |
| CN110420164A (en) * | 2019-09-29 | 2019-11-08 | 国佳凝胶科创中心(深圳)有限公司 | A kind of snail essence dry powder and the preparation method and application thereof |
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