CN1119534A - Improved technology for extracting whole snail nutrient liquor - Google Patents
Improved technology for extracting whole snail nutrient liquor Download PDFInfo
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- CN1119534A CN1119534A CN 94115248 CN94115248A CN1119534A CN 1119534 A CN1119534 A CN 1119534A CN 94115248 CN94115248 CN 94115248 CN 94115248 A CN94115248 A CN 94115248A CN 1119534 A CN1119534 A CN 1119534A
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- limax
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Abstract
The present invention relates to a technological process for improving the prepration of whole snail nutrient extract. In the step of preparation of minced snail meat the edible proteinase is added to make enzymolysis, thus increasing the protein and amino acid contents in the extract, and reducing their loss, at the same time the oligopeptide and amino acids are very easily absorbed by human body through skin, therefore the application value of the cosmetics using said extract as matrix is greatly raised, and the utilization ratio of snail can be fully raised, and its cost can be reduced.
Description
The present invention relates to the extracting method of the animal extracts of cosmetic field, the application of medicines and health protection field, be specially the method that snail nutrient liquor extracts.
Mucin of Limax and protein content shelter have first of the animal, and contain abundant special composition, as mucin, multiple human body can't synthetic aminoacid, fatty acid, organic acid, vitamin, the trace element of the enzyme of more than 30 kind of biologically active and immunocompetence thing, several mineral materials and needed by human body: the natural nutrition composition of diversification such as selenium, zinc, boron, calcium, phosphorus, ferrum.Therefore it is a kind of nutritious traditional food and has important medical value.Except that as the food, its extract has been applied in medicine and cosmetic field, and is more and more attracted attention by common people at present.CN1086424A discloses a kind of manufacture method of the whole snail nutrient liquor that can be used for producing in batches, but the Limax cattle nutrition extracting solution that this method is produced, wherein protein and amino acid whose extraction ratio are still lower, after hydrolysis finishes, still remain more Limax raw material, therefore not high enough to the utilization rate of Limax, make production cost higher, be unfavorable for applying more widely.
Purpose of the present invention, for overcoming the shortcoming of prior art, a kind of method of improved extraction snail nutrient liquor is provided, to improve protein and amino acid whose content in the snail nutrient liquor, improve its nutrient content on the whole, thereby improve the utilization rate of Limax, reduce the cost of making the snail nourishing extracting solution to reach.
The improvement technology of making Limax cattle nutrition extracting solution of the present invention is in the method step that CN1086424A provides, with the Carnis Limax after extracting, be twisted into minced meat, in extracting in water, add the edible protein enzyme of the heavy 1-2% of minced meat,, increased aminoacid and Protein content in the extracting solution greatly through the Carnis Limax extracting solution that enzymolysis makes, that is to say through enzymolysis, protein and aminoacid extraction ratio in the Carnis Limax are improved.Thereby protein and amino acid whose content have improved greatly in the Limax cattle nutrition extracting solution that makes the extracting solution of merging Carnis Limax, minced meat, internal organs and make.Its concrete processing step is as follows:
1, selected Limax:
Every artificial breeding's Limax all can be selected for use.
The requirement nothing is ill, active, body is strong, healthy.
Every weight can be selected for use more than 10 grams, with every heavy 30-60 gram better.
Can not select frowziness for use, shell and dead Limax contract.
The selected plastic box of putting into the aperture of bottom as the Limax of extracting usefulness is rested, and every case keeps flat at most into one deck to well, can not pile pressure.
The Limax that will live rested in hungry 48-72 hour, no longer fed and let alone what feedstuff, and Limax has drained food and the feces in the clean the intestines and stomach substantially after resting.
2, slaughtering and the separation of meat, internal organs of Limax:
The Limax that rests is rinsed well with water purification, reuse bleaching powder water or the flushing of nontoxic antibacterial one time, to go out except that the antibacterial of Limax surface, quiet again putting 4 hours after pulling out treats can slaughter after calcium hypochlorite (bleaching powder) vapors away fully.
Limax is placed on the operating board, Crusta Limax is broken into pieces, note not striking bad meat and internal organs, firmly want evenly when striking with the lumps of wood.
Peel off broken shell, along Limax internal organs marginal portion internal organs are cut, complete meat is separated also placed apart with internal organs with the rustless steel shears.
3, the operating process of Carnis Limax and internal organs extraction:
3, the extraction of 1 Carnis Limax
3,1,1 meat put into the container that band valve in bottom has agitating device, by Carnis Limax: water is 1: 1-4 (weight), and described water can be used deionized water, distilled water, mixing speed is 150-200 rev/min, be advisable with 180 rev/mins, mixing time is 30 minutes, temperature is controlled at≤and 40 ℃; Then, put that clean to dissolve the whole extracting solution that standby; Reform 4 times by above method and step, merge extractive liquid, adds 0.2%LI911 antiseptic standing over night again.
3,1,2 Carnis Limaxs of telling after the dissolving, rub into minced meat with machinery, weigh, put into agitated kettle, by the Limax minced meat: water is 1: 1-4 (weight) adds water, be heated to 80 ℃-100 ℃ and boiled 20-30 minute, carry out high temperature sterilize, natural cooling, again temperature is controlled at 37 ℃-45 ℃, accurately measure pH value, and pH value is controlled at 6.5-7.0, add in the edible neutral protease of 1-2% of minced meat weight or the acid protease any, stir by above-mentioned mixing speed, through enzymolysis 5-8 hour, this moment, the minced meat slag was run out of by enzymolysis basically.Be warmed up to 65 ℃ of enzyme denaturing (perhaps transferring the PH5.0 enzyme denaturing) then with vitamin C.Cross 200 mesh sieves hydrolyzed solution is put storage vat, weigh 15 times water dilution, add LI911 antiseptic 0.2% stand for standby use with former minced meat.Described protease is to adopt sino-america joint-venture Wuxi star to reach biological engineering company limited product, enzyme activity (U/g): 100,000.
3,2 Limax internal organs extract:
Extract albuminous glands part in the Limax internal organs, by Carnis Limax: 5: 1 ratio of Limax internal organs (weight) takes by weighing the Limax internal organs, operating process same 3,1,1.The gained extracting solution adds 0.2%LI911 antiseptic, stand for standby use.
3,3 extracting solution heat sterilizations:
Carnis Limax, minced meat, the internal organs lysate of above preparation are merged after coarse filtration machine coarse filtration, and the polysulfone hollow fibre super Lu device heat sterilization of back by 8-15 ten thousand molecular weight stirs.Best with the polysulfone hollow fibre of selecting 100,000 molecular weight.The degerming rate can reach 100%, guarantees that simultaneously the snail nourishing extracting solution filters whole active ingredients of needed by human body, does not destroy the Snailase activity.
Show after testing, with improvement technology of the present invention, the enzymolysis process that promptly in the extraction process of Limax minced meat, adds protease, can improve amino acid content (table 1) and Protein content (table 2) in the snail extract significantly, reduced loss, because low peptide and the aminoacid utmost point again are beneficial to the human body Transdermal absorption, having improved with this extracting solution is the cosmetics use value of substrate; Owing to made full use of whole Carnis Limaxs, improved the utilization rate of Limax, therefore can reduce cost, help applying to wideer field.
Table 1 adds enzymatic process
*With do not add enzymatic process
*Amino acid content in the extracting solution
| The aminoacid title | The Limax minced meat add zyme extract (mg/100ml) | Carnis Limax does not add zyme extract (mg/100ml) |
| Lucid asparagus is pacified sour threonine serine glutamic acid proline glycine alanine cystine valine methionine isoleucine leucine tyrosine phenylalanine lysine ammonia histidine arginine | ????64.37 ????33.64 ????31.87 ????109.59 ????24.77 ????215.08 ????47.03 ????5.86 ????39.79 ????16.08 ????48.56 ????59.60 ????33.63 ????48.19 ????17.94 ????4.08 ????48.04 | ????18.91 ????8.63 ????8.41 ????19.59 ????7.86 ????82.40 ????9.65 ????2.11 ????8.45 ????2.13 ????11.27 ????13.12 ????0.21 ????9.89 ????6.78 ????5.27 ????5.32 ????8.63 |
| Total content (does not comprise NH 3) | ????830.18 | ????223.36 |
Table 2 adds enzymatic process and does not add protein content in the enzymatic process extracting solution
| Add enzymatic process (minced meat) ** | Not enzyme-added (meat and minced meat) * | |
| Protein (%) | ????1.65 | ????0.43 |
*With the 10Kg Carnis Limax, at agitated kettle meat, add the 20Kg deionized water, stirred 30 minutes at 40 ℃ with 180 rev/mins of speed, it is standby in material-storage jar to put clean extracting solution; Heavy again coated with last operation 4 times, merge 5 extracting solution 100Kg altogether, what add that the 220gLI911 antiseptic makes does not add the enzymatic process extracting solution.
*Will
*The 10Kg Carnis Limax, the Carnis Limax after not enzyme-added preliminary hydrolyzed extract, the 8Kg that weighs after rubbing adds the 16Kg deionized water, is heated to 85 ℃-95 ℃ and keeps 20 minutes, cools to 40 ℃, in PH
* *Surveying PH is 6.8, adds 8g (1%) neutral protease, and with 180 rev/mins of stirrings 8 hours, adjusting PH with vitamin C then was that 5.0 (measuring with the PH reagent paper) are added the 64Kg deionized water again.Make the Limax minced meat that add enzymatic process and add zyme extract.
* *PH counts PHs-25 numeral acidometer.
Example 1:
1, select for use every weight to restrain 10 kilograms of healthy active Limaxs at 40-50, after hunger was rested 48 hours, with 20 ℃ of left and right sides flushing with clean water one time, place after 4 hours, Limax is put on the operating board, shell is broken into pieces with the lumps of wood, avoid breaking up internal organs as far as possible, peel off broken shell, cut off with the marginal portion that internal organs connect along Limax with the rustless steel shears, internal organs and meat are placed apart.Isolating 3.5 kilograms of Carnis Limaxs are put into the rustless steel container that agitating device, bottom band valve are arranged at the top, add 7 kilograms of deionized waters, be incubated 40 ℃ of stirrings 30 minutes, 180 rev/mins of mixing speeds, whole liquid of putting after the clean dissolving are standby; Repeat to extract 4 times by above method step again, altogether 35 kilograms of extracting solution, add 70 gram LI911 antiseptic.
2, telling Carnis Limax rubs with meat grinder, cross title, 2 kilograms of Carnis Limaxs, put back to agitated kettle, add 4 kilograms of deionized waters and be heated to 100 ℃ and boiled 20 minutes, be cooled to 40 ℃ again, transfer about PH6.7, add food industry, be incubated 40 ℃ with neutral protease 2 grams (sino-america joint-venture Wuxi star reaches the biological engineering company limited and produces), 180 rev/mins of mixing speeds, enzymolysis 8 hours.Transferring pH value again with vitamin C then is 5.0 enzyme denaturing, crosses 200 mesh sieve elimination residues, and hydrolyzed solution is put storage tank, adds 34 kilograms of deionized waters, adds 68 gram LI911 antiseptic again.
3, extract the dirty albuminous glands part of Carnis Limax, take by weighing 0.7 kilogram in internal organs, add agitated kettle, add 1.4 kilograms of deionized waters, be incubated 37 ℃, 30 minutes, 180 rev/mins of mixing speeds are put clean extracting solution, and were standby, and repetitive operation gets 7 kilograms of internal organs extracting solution 4 times again.
4, the extracting solution of above three step gained is amounted to 76 kilograms and mix after coarse filtration machine coarse filtration, the same heat sterilization of polysulfone hollow fibre ultrafiltration by 100,000 molecular weight promptly gets aseptic nutrition extracting solution.
Example 2
Operating procedure example 1, transferring PH is 3.5, replaces neutral protease with edible industrial acid protease (sino-america joint-venture Wuxi star reaches the biological engineering company limited and produces), transferring pH value with triethanolamine is 6.5 enzyme denaturing, makes aseptic Limax cattle nutrition extracting solution.
Example 3
Operating procedure is transferred the pH value enzyme denaturing with example 1 with being warmed to 65 ℃ of replacements in 30 minutes, makes aseptic Limax cattle nutrition extracting solution.
Claims (4)
1, a kind of method of making Limax cattle extracting solution, selected active, healthy and strong Limax is through slaughtering, to meat and the internal organs making extracting solution that is dissolved in water respectively, Carnis Limax after will dissolving again is twisted into the minced meat extracting in water, it is characterized in that in the minced meat extracting in water, add the edible protein enzyme of the heavy 1-2% of minced meat, through enzymolysis, make extracting solution, merge meat, minced meat, internal organs extracting solution, form through ultrafiltration, described edible protein enzyme be in neutral protease or the acid protease any.
2, the method for making Limax cattle extracting solution as claimed in claim 1 is characterized in that the concrete processing step of described minced meat enzymolysis is as follows:
(2.1) with minced meat by 1: 1-4 (weight) adds water, is heated to 80 ℃-100 ℃, after 20-30 minute, the cooling;
(2.2) the control temperature is in the time of 37 ℃-45 ℃, and regulating pH value is that 6.5-7.0 adds the edible neutral protease by the heavy 1-2% of minced meat, stirs enzymolysis 5-8 hour;
(2.3) be warmed up to 65 ℃ of enzyme denaturing, cross 200 mesh sieves, remove residue;
(2.4) weigh 15 times of water dilutions with minced meat;
(2.5) adding preservative agent
3, the method for making Limax cattle extracting solution as claimed in claim 1 is characterized in that replacing neutral protease with acid protease, and described pH value is 3.5.
4, as the method for the described making Limax of claim 2.3 cattle extracting solution, it is characterized in that the described 65 ℃ of enzyme denaturing that are warming up to, available accent pH value replaces for the 6-7 enzyme denaturing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94115248 CN1119534A (en) | 1994-09-29 | 1994-09-29 | Improved technology for extracting whole snail nutrient liquor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94115248 CN1119534A (en) | 1994-09-29 | 1994-09-29 | Improved technology for extracting whole snail nutrient liquor |
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| Publication Number | Publication Date |
|---|---|
| CN1119534A true CN1119534A (en) | 1996-04-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 94115248 Pending CN1119534A (en) | 1994-09-29 | 1994-09-29 | Improved technology for extracting whole snail nutrient liquor |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1054505C (en) * | 1996-12-11 | 2000-07-19 | 林镇才 | Active constitution cosmetic additive |
| CN1101685C (en) * | 1998-12-25 | 2003-02-19 | 海南雄风蜗牛保健品有限公司 | Snail health products making process |
| CN107475342A (en) * | 2017-10-17 | 2017-12-15 | 陈和平 | A kind of extracting method of snail active peptide |
-
1994
- 1994-09-29 CN CN 94115248 patent/CN1119534A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1054505C (en) * | 1996-12-11 | 2000-07-19 | 林镇才 | Active constitution cosmetic additive |
| CN1101685C (en) * | 1998-12-25 | 2003-02-19 | 海南雄风蜗牛保健品有限公司 | Snail health products making process |
| CN107475342A (en) * | 2017-10-17 | 2017-12-15 | 陈和平 | A kind of extracting method of snail active peptide |
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