CN107019111B - Composition for improving yield of oocysts of eimeria maxima and preparation method thereof - Google Patents
Composition for improving yield of oocysts of eimeria maxima and preparation method thereof Download PDFInfo
- Publication number
- CN107019111B CN107019111B CN201610066117.9A CN201610066117A CN107019111B CN 107019111 B CN107019111 B CN 107019111B CN 201610066117 A CN201610066117 A CN 201610066117A CN 107019111 B CN107019111 B CN 107019111B
- Authority
- CN
- China
- Prior art keywords
- composition
- eimeria maxima
- oocysts
- parts
- vitamin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/002—Protozoa antigens
- A61K39/012—Coccidia antigens
Abstract
The invention relates to a composition for improving the oocyst yield of eimeria maxima and a preparation method thereof, wherein the composition comprises the following components in parts by weight: 10-30 parts of Chinese pulsatilla root; 1-10 parts of vitamin A; 0.5-10 parts of vitamin E; 2-30 parts of berberine; 0.5-5 parts of sodium cholate; 10-90 parts of stone powder. The composition has scientific and reasonable composition and simple process, can improve the yield of the oocysts of the eimeria maxima by maintaining the integrity of the epithelial mucosa of the intestinal tract, reducing the damage of gram-negative bacteria of the intestinal tract to the epithelial cells of the intestinal tract, increasing the cyst removal rate of sporozoites and the like, and can improve the production efficiency of the eimeria maxima vaccine, reduce the production cost and save social resources.
Description
Technical Field
The invention belongs to the field of biological products for livestock, and particularly relates to a composition for improving the yield of oocysts of eimeria maxima and a preparation method thereof.
Background
In China and all over the world, chicken coccidiosis is a common parasitic disease which is the most painful for chicken practitioners and has great harm. At present, the disease is mainly prevented and treated by chemical medicines, the medicine cost is high, the environment pollution is caused in the production and application processes, particularly, the flowing of poultry meat and eggs containing medicine residues to a dining table greatly threatens the body health of people, and under the concept of 'prevention over treatment', the value of coccidian vaccines is more and more accepted by people. The high and low oocyst yield is directly related to the coccidian vaccine quality and the production cost thereof, so that how to improve the coccidian oocyst yield becomes a main research direction for practitioners of veterinary biological products.
Disclosure of Invention
The invention aims to provide a composition for improving the yield of oocysts of eimeria maxima and a preparation method thereof. The composition has scientific and reasonable composition and simple process, can improve the yield of the oocysts of the eimeria maxima by maintaining the integrity of the epithelial mucosa of the intestinal tract, reducing the damage of gram-negative bacteria of the intestinal tract to the epithelial cells of the intestinal tract, increasing the cyst removal rate of sporozoites and the like, and can improve the production efficiency of the eimeria maxima vaccine, reduce the production cost and save social resources.
Another object of the present invention is a composition for increasing the production of Eimeria maxima oocysts and a method for preparing the same.
The invention is realized by the following technical scheme:
a composition for improving the oocyst yield of Eimeria maxima and a preparation method and application thereof are disclosed, wherein the composition comprises the following components in parts by weight:
moreover, the components of the composition and the optimal weight part ratio thereof are respectively as follows:
a composition for improving the oocyst yield of Eimeria maxima and a preparation method thereof are disclosed, wherein the preparation method comprises the following steps:
(1) weighing the Chinese pulsatilla root according to the weight ratio, crushing, sieving by a 80-mesh sieve, and putting into a mixing tank for later use;
(2) respectively weighing vitamin A, vitamin E, berberine and sodium cholate according to the weight ratio, sequentially putting the vitamin A, the vitamin E, the berberine and the sodium cholate into the mixing tank filled with the Chinese pulsatilla root in the step (1), and premixing for 20 minutes to obtain a mixture A for later use;
(3) weighing stone powder according to the weight ratio, crushing, sieving with a 80-mesh sieve, then placing into a mixing drying oven, drying for 1 hour at 80 ℃, and cooling to room temperature;
(4) and (3) putting the stone powder cooled to room temperature in the step (3) into a mixing tank filled with the mixture A obtained in the step (2), and mixing for 30 minutes. Mixing, packaging into aluminum foil bags, and sealing to obtain the composition for increasing the yield of Eimeria maxima oocysts.
The invention has the advantages and beneficial effects that:
1. the invention aims to provide a composition for improving the yield of oocysts of eimeria maxima and a preparation method thereof. The invention has scientific and reasonable formula composition, simple process, convenient use and lower cost.
2. The invention utilizes the pulsatilla chinensis and the berberine to reduce the damage of gram-negative bacteria in the intestinal tract to the epithelial cells of the intestinal tract and maintain the integrity of the epithelial mucosa of the intestinal tract so as to improve the yield of the oocysts of the eimeria maxima.
3. The invention utilizes the vitamin A and the vitamin E to improve the activity of the epithelial cells of the intestinal tract and maintain the integrity of the epithelial mucosa of the intestinal tract to improve the yield of the oocysts of the Eimeria maxima.
4. The method utilizes the sodium cholate to increase the excystation rate of the sporozoites and increase the constant value probability of the sporozoites on the intestinal mucosa to improve the yield of the oocysts of the Eimeria maxima.
Detailed Description
The invention is further illustrated by the following examples. It should be noted that: the following examples are illustrative and not intended to be limiting, and are not intended to limit the scope of the invention.
The components of the Chinese pulsatilla root, the vitamin A, the vitamin E, the berberine, the sodium cholate and the stone powder can be purchased in the market.
Example 1
A composition for improving the oocyst yield of Eimeria maxima and a preparation method and application thereof are disclosed, wherein the composition comprises the following components in parts by weight: 10kg of Chinese pulsatilla root, 1kg of vitamin A, 0.5kg of vitamin E, 2kg of berberine, 0.5kg of sodium cholate and 10kg of stone powder.
A composition for improving the oocyst yield of Eimeria maxima and a preparation method thereof are disclosed, wherein the preparation method comprises the following steps:
(1) weighing the Chinese pulsatilla root according to the weight ratio, crushing, sieving by a 80-mesh sieve, and putting into a mixing tank for later use;
(2) respectively weighing vitamin A, vitamin E, berberine and sodium cholate according to the weight ratio, sequentially putting the vitamin A, the vitamin E, the berberine and the sodium cholate into the mixing tank filled with the Chinese pulsatilla root in the step (1), and premixing for 20 minutes to obtain a mixture A for later use;
(3) weighing stone powder according to the weight ratio, crushing, sieving with a 80-mesh sieve, then placing into a mixing drying oven, drying for 1 hour at 80 ℃, and cooling to room temperature;
(4) and (3) putting the stone powder cooled to room temperature in the step (3) into a mixing tank filled with the mixture A obtained in the step (2), and mixing for 30 minutes. Mixing, packaging into aluminum foil bags, and sealing to obtain the composition for increasing the yield of Eimeria maxima oocysts.
Example 2
A composition for improving the oocyst yield of Eimeria maxima and a preparation method and application thereof are disclosed, wherein the composition comprises the following components in parts by weight: 3kg of Chinese pulsatilla root, 1kg of vitamin A, 1kg of vitamin E, 3kg of berberine, 0.5kg of sodium cholate and 9kg of stone powder.
The preparation and use are as in example 1.
Example 3
A composition for improving the oocyst yield of Eimeria maxima and a preparation method and application thereof are disclosed, wherein the composition comprises the following components in parts by weight: 2kg of Chinese pulsatilla root, 0.5kg of vitamin A, 0.1kg of vitamin E, 2kg of berberine, 0.2kg of sodium cholate and 5kg of stone powder.
The preparation and use are as in example 1.
Clinical application
The invention is used according to the following method: the composition is fed to the breeding hens to be inoculated from 8 days before the inoculation of the eimeria maxima oocyst vaccine strain, 100kg of the composition is mixed with 100g of the feed every 100g of the composition, and the feeding is carried out for 3 days at intervals of 2 days and then for 3 days.
Production test:
200 healthy AA broiler chickens infected by coccidian of 10 days old are selected and randomly divided into 4 groups, namely a high-dose group, a medium-dose group, a low-dose group and a blank control group, and 50 broiler chickens in each group are respectively treated as follows.
High dose group: feeding composition 1 at 11-13 days old and 16-18 days old, respectively, and mixing 100kg of the composition per 100 g. At 19 days of age, 2 ten thousand oocysts per egg were inoculated with the E.maxima oocyst vaccine strain. Feces of 26-30 days of age were harvested and oocysts were extracted therefrom.
The medium dose group: feeding composition 2 at 11-13 days old and 16-18 days old, respectively, and mixing 100kg of the composition per 100 g. At 19 days of age, 2 ten thousand oocysts per egg were inoculated with the E.maxima oocyst vaccine strain. Feces of 26-30 days of age were harvested and oocysts were extracted therefrom.
Low dose group: feeding composition 3 at 11-13 days old and 16-18 days old, respectively, and mixing 100kg of the composition per 100 g. At 19 days of age, 2 ten thousand oocysts per egg were inoculated with the E.maxima oocyst vaccine strain. Feces of 26-30 days of age were harvested and oocysts were extracted therefrom.
Blank control group: normally feeding without any medicine. At 19 days of age, 2 ten thousand oocysts per egg were inoculated with the E.maxima oocyst vaccine strain. Feces of 26-30 days of age were harvested and oocysts were extracted therefrom.
And (3) test results:
oocyst production in high dose group: 7302 ten thousand oocysts per chicken;
oocyst yield in medium dose group: 6430 ten thousand oocysts per chicken;
oocyst production in low dose groups: 5910 ten thousand oocysts per chicken;
oocyst yield of blank control group: 4800 Ten thousand oocysts per chicken.
And (4) analyzing results: the Eimeria maxima oocysts fed to the high dose group of chickens were 13.6% higher, 23.6% higher, and 40.0% higher than the control group of chickens fed the low and medium dose groups, respectively. The above data demonstrate that the composition of the present invention has a significant effect on increasing the oocyst yield of eimeria maxima, and can greatly increase the production efficiency of eimeria maxima vaccines.
Claims (3)
1. A composition for increasing the oocyst yield of Eimeria maxima, comprising: the composition comprises the following components in parts by weight:
2. the composition of claim 1, wherein the composition for increasing the production of oocysts of eimeria maxima is: the composition comprises the following components in parts by weight:
3. a method of preparing the composition of claim 1 for increasing the production of oocysts of eimeria maxima, comprising: the preparation method comprises the following steps:
(1) weighing the Chinese pulsatilla root according to the weight ratio, crushing, sieving by a 80-mesh sieve, and putting into a mixing tank for later use;
(2) respectively weighing vitamin A, vitamin E, berberine and sodium cholate according to the weight ratio, putting the vitamin A, the vitamin E, the berberine and the sodium cholate into the mixing tank with the Chinese pulsatilla root in the step (1) once, and premixing for 20 minutes to obtain a mixture A for later use;
(3) weighing stone powder according to the weight ratio, crushing, sieving with a 80-mesh sieve, then placing into a mixing drying oven, drying for 1 hour at 80 ℃, and cooling to room temperature;
(4) putting the stone powder cooled to room temperature in the step (3) into a mixing tank filled with the mixture A obtained in the step (2), and mixing for 30 minutes; mixing, packaging into aluminum foil bags, and sealing to obtain the composition for increasing the yield of Eimeria maxima oocysts.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610066117.9A CN107019111B (en) | 2016-01-31 | 2016-01-31 | Composition for improving yield of oocysts of eimeria maxima and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610066117.9A CN107019111B (en) | 2016-01-31 | 2016-01-31 | Composition for improving yield of oocysts of eimeria maxima and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107019111A CN107019111A (en) | 2017-08-08 |
| CN107019111B true CN107019111B (en) | 2020-11-20 |
Family
ID=59524225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610066117.9A Active CN107019111B (en) | 2016-01-31 | 2016-01-31 | Composition for improving yield of oocysts of eimeria maxima and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107019111B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107912643B (en) * | 2017-12-31 | 2021-04-20 | 天津赫莱恩特生物科技有限公司 | Composition for improving extraction rate of oocysts of eimeria maxima and preparation method thereof |
| CN107927431B (en) * | 2017-12-31 | 2021-04-20 | 天津赫莱恩特生物科技有限公司 | Composition for reducing impurities in eimeria maxima vaccine and preparation method |
| CN108148761A (en) * | 2017-12-31 | 2018-06-12 | 天津赫莱恩特生物科技有限公司 | A kind of composition for improving Eimeria Tenella egg capsule yield and preparation method thereof |
| CN108060085A (en) * | 2017-12-31 | 2018-05-22 | 天津赫莱恩特生物科技有限公司 | It is a kind of to improve the method for poisoning eimeria tenella egg capsule purifying success rate |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009148895A1 (en) * | 2008-05-29 | 2009-12-10 | Intervet International B.V. | Coccidiosis vaccines |
| CN102796669A (en) * | 2012-08-07 | 2012-11-28 | 广西壮族自治区兽医研究所 | Method for extracting and purifying eimeria tenella merozoite |
| CN102845607A (en) * | 2012-08-06 | 2013-01-02 | 王茜 | Novel antibacterial antiviral feed additive |
-
2016
- 2016-01-31 CN CN201610066117.9A patent/CN107019111B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009148895A1 (en) * | 2008-05-29 | 2009-12-10 | Intervet International B.V. | Coccidiosis vaccines |
| CN102845607A (en) * | 2012-08-06 | 2013-01-02 | 王茜 | Novel antibacterial antiviral feed additive |
| CN102796669A (en) * | 2012-08-07 | 2012-11-28 | 广西壮族自治区兽医研究所 | Method for extracting and purifying eimeria tenella merozoite |
Non-Patent Citations (2)
| Title |
|---|
| 球虫疫苗稀释剂的制备及其对疫苗免疫效果的影响;朱晓波;《中国优秀硕士学位论文全文数据库 农业科技辑》;20150215;第1-43页 * |
| 鸡球虫病的辅助性治疗措施;王洪鹏;《当代畜牧》;20031231(第2期);第10页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107019111A (en) | 2017-08-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10265359B2 (en) | Feed additive for swine and feed composition containing the same | |
| CN107019111B (en) | Composition for improving yield of oocysts of eimeria maxima and preparation method thereof | |
| CN102132780B (en) | Feed additive for chickens | |
| Guo et al. | Coccidiosis immunization: effects of mushroom and herb polysaccharides on immune responses of chickens infected with Eimeria tenella | |
| CN107022487B (en) | Preparation method and application of coccidian oocyst activity protective agent in chicken manure | |
| CN102895378B (en) | Pharmaceutical compound preparation for treating livestock respiratory diseases and method for preparing pharmaceutical compound preparation for treating livestock respiratory diseases | |
| Shen et al. | Effect of echinacea purpurea extract given in drinking water on performance, slaughter variables, and meat quality of broilers | |
| CN106562110A (en) | Chinese herbal medicinal feed additive composition for salvelinus malma and preparation method and use thereof | |
| CN105104890B (en) | It is a kind of promote sow Oocyte Meiosis restore composition and application | |
| CN105746846B (en) | South dodder seed and motherwort herb pharmaceutical composition, and premix, concentrate, batch and application thereof | |
| CN104288098B (en) | A kind of preparation method of decoquinate ester solution | |
| CN104758516B (en) | Eupatorium adenophorum composition for treating chicken coccidiosis and preparation method thereof | |
| CN103623079A (en) | Preparation method and use of spine date seed extract having effects of protecting liver and bladder and improving immunity | |
| CN1907337A (en) | Shuanghuanglian oral liquor for fowl and its preparing method | |
| CN106974936A (en) | A kind of composition for improving broiler chicken coccidial vaccine immune effect and preparation method thereof | |
| CN106615922A (en) | Feed for reducing attack rate of inclusion body hepatitis of broilers | |
| CN102363581A (en) | Biological organic fertilizer and preparation method thereof | |
| KR100962231B1 (en) | Pig feed additive using ginseng leaf and stem extract | |
| CN105687648B (en) | Traditional Chinese medicine composition for poultry, feed additive and premix | |
| CN106983861B (en) | Composition for accelerating in-vivo excystation of chicken coccidian oocysts and preparation method thereof | |
| CN104666429A (en) | Composition for treating proventriculitis of chicken and preparation method of composition | |
| CN106689713A (en) | Feed additive for meat goats | |
| CN107019799A (en) | A kind of application of chicken coccidiasis tetravalence attenuated live vaccines composition in production | |
| CN102688477A (en) | Medicinal composition for treating respiratory diseases of livestock | |
| CN108653300B (en) | Pharmaceutical composition for purifying seed of salmonella gallinarum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |