CN107912643B - Composition for improving extraction rate of oocysts of eimeria maxima and preparation method thereof - Google Patents
Composition for improving extraction rate of oocysts of eimeria maxima and preparation method thereof Download PDFInfo
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- CN107912643B CN107912643B CN201711494600.8A CN201711494600A CN107912643B CN 107912643 B CN107912643 B CN 107912643B CN 201711494600 A CN201711494600 A CN 201711494600A CN 107912643 B CN107912643 B CN 107912643B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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Abstract
The invention relates to a composition for improving the extraction rate of oocysts of eimeria maxima and a preparation method thereof, wherein the composition comprises the following components in parts by weight: 30-65 parts of glucose, 25-40 parts of yolk powder and 0.2-2 parts of vitamin A; 0.1-1 part of vitamin E; 0.4-4 parts of vitamin D. The invention has scientific and reasonable formula composition, simple process, convenient use and lower cost. The composition can reduce the sampling amount of the excrement, improve the oocyst yield of the eimeria maxima oocysts extracted from the excrement, greatly improve the extraction efficiency of the eimeria maxima oocysts, reduce the expenditure of manpower, material resources and energy consumption and reduce the production cost.
Description
Technical Field
The invention belongs to the field of biological products for livestock, and particularly relates to a composition for improving the extraction rate of oocysts of eimeria maxima and a preparation method thereof.
Background
In the production of coccidian vaccines, the Eimeria maxima oocysts are mainly extracted from chicken manure, and the process is time-consuming and labor-consuming, so that how to improve the extraction efficiency of the Eimeria maxima oocysts becomes a subject which needs to be researched urgently, and the research is carried out by the invention.
Disclosure of Invention
The invention aims to provide a composition for improving the extraction rate of oocysts of eimeria maxima and a preparation method thereof, wherein the composition comprises the following components in parts by weight: 30-65 parts of glucose; 25-40 parts of egg yolk powder; 0.2-3 parts of vitamin A; 0.1-2 parts of vitamin E; vitamin D30.4-5 parts. The invention has scientific and reasonable formula composition, simple process, convenient use and lower cost. The composition can reduce the discharge amount of excrement, improve the yield of oocysts of eimeria maxima extracted from the excrement, greatly improve the extraction efficiency of the eimeria maxima oocysts, reduce the expenditure of manpower, material resources and energy consumption and reduce the production cost.
Another object of the present invention is to provide a composition for increasing the extraction rate of Eimeria maxima oocysts and a method for preparing the same.
The invention is realized by the following technical scheme:
a composition for improving the extraction rate of oocysts of Eimeria maxima and a preparation method thereof are disclosed, wherein the composition comprises the following components in parts by weight:
30-65 parts of glucose;
25-40 parts of egg yolk powder;
0.2-3 parts of vitamin A;
0.1-2 parts of vitamin E;
vitamin D30.4-5 parts.
Moreover, the components of the composition and the optimal weight part ratio thereof are respectively as follows:
60 parts of glucose;
35 parts of egg yolk powder;
2 parts of vitamin A;
1 part of vitamin E;
vitamin D3And 3 parts.
A composition for improving the extraction rate of oocysts of Eimeria maxima and a preparation method thereof are disclosed, wherein the preparation method comprises the following steps:
(1) respectively weighing vitamin A, vitamin E and vitamin D according to the weight ratio3Adding into a mixing tank, and premixing for 10 minutes for later use.
(2) And weighing glucose according to the weight ratio, and putting the glucose into a mixing tank filled with the premix to mix for 20 minutes for later use.
(3) Weighing yolk powder according to the weight ratio, and putting the yolk powder into a mixing tank filled with the premix to mix for 30 minutes. Mixing, packaging into aluminum foil bags, and sealing. Thus obtaining the composition for improving the extraction rate of the oocysts of the eimeria maxima.
The composition for improving the extraction rate of the oocysts of the eimeria maxima is applied to the production of coccidian vaccines by the following method: starting 5 days after the E.maxima oocyst vaccine strain is inoculated, stopping feeding the breeding hens with the feed, feeding the composition of the invention freely until the production is finished.
The invention has the advantages and beneficial effects that:
1. the invention aims to provide a composition for improving the extraction rate of oocysts of eimeria maxima and a preparation method thereof. The invention has scientific and reasonable formula composition, simple process, convenient use and lower cost.
2. The method utilizes the glucose to meet the requirement of the chicken on energy, reduces the discharge amount of the excrement, and improves the efficiency of extracting the oocysts of the eimeria maxima from the excrement.
3. The egg yolk powder is used for meeting the requirements of the chicken on protein, the protein density is high, the absorption rate is high, the excrement discharge amount is small, the content of particulate matters in excrement can be reduced, and the efficiency of extracting the Eimeria maxima oocysts from the excrement is improved.
Detailed Description
The invention is further illustrated by the following examples. It should be noted that: the following examples are illustrative and not intended to be limiting, and are not intended to limit the scope of the invention.
The components of the invention comprise glucose, yolk powder, vitamin A, vitamin E and vitamin D3Are commercially available.
Example 1
A composition for improving the extraction rate of oocysts of Eimeria maxima and a preparation method thereof are disclosed, wherein the composition comprises the following components in parts by weight: 60kg of glucose, 35kg of egg yolk powder, 2kg of vitamin A, 1kg of vitamin E and D3 3kg。
A composition for improving the extraction rate of oocysts of Eimeria maxima and a preparation method thereof are disclosed, wherein the preparation method comprises the following steps:
(1) respectively weighing vitamin A, vitamin E and vitamin D according to the weight ratio3Adding into a mixing tank, and premixing for 10 minutes for later use.
(2) And weighing glucose according to the weight ratio, and putting the glucose into a mixing tank filled with the premix to mix for 20 minutes for later use.
(3) Weighing yolk powder according to the weight ratio, and putting the yolk powder into a mixing tank filled with the premix to mix for 30 minutes. Mixing, packaging into aluminum foil bags, and sealing. Thus obtaining the composition for improving the extraction rate of the oocysts of the eimeria maxima.
The composition for improving the extraction rate of the oocysts of the eimeria maxima is applied to the production of coccidian vaccines by the following method: starting 5 days after the E.maxima oocyst vaccine strain is inoculated, stopping feeding the breeding hens with the feed, feeding the composition of the invention freely until the production is finished.
Example 2
A composition for improving the extraction rate of oocysts of Eimeria maxima and a preparation method thereof are disclosed, wherein the composition comprises the following components in parts by weight: 55kg of glucose, 40kg of egg yolk powder, 1kg of vitamin A, 1kg of vitamin E and 1kg of vitamin D3 4kg。
The preparation and use are as in example 1.
Example 3
A composition for improving the extraction rate of oocysts of Eimeria maxima and a preparation method thereof are disclosed, wherein the composition comprises the following components in parts by weight: 50kg of glucose, 45kg of egg yolk powder, 1kg of vitamin A, 2kg of vitamin E and D3 3kg。
The preparation and use are as in example 1.
Clinical application
First, 3 compositions were prepared:
composition 1: prepared according to the method of example 1;
composition 2: prepared as in example 2;
composition 3: prepared according to the method of example 3;
production test:
200 healthy AA broiler chickens infected by coccidian of 20 days old are selected, 5 ten thousand oocysts of the Eimeria maxima oocyst vaccine strain are inoculated, and the inoculated healthy AA broiler chickens are randomly divided into 4 groups of 50 chickens.
Group one: composition 1 was started at 26 days of age and consumed ad libitum. Collecting feces of 26-29 days old, weighing, measuring OPG, calculating total yield, extracting oocysts, and calculating oocyst yield.
And a second group: composition 2 was started at 26 days of age and consumed ad libitum. Collecting feces of 26-29 days old, weighing, measuring OPG, calculating total yield, extracting oocysts, and calculating oocyst yield.
And (3) group III: composition 3 was started at 26 days of age and consumed ad libitum. Collecting feces of 26-29 days old, weighing, measuring OPG, calculating total yield, extracting oocysts, and calculating oocyst yield.
Group four: after inoculation, normal feed is fed, and the feed is taken freely. Collecting feces of 26-29 days old, weighing, measuring OPG, calculating total yield, extracting oocysts, and calculating oocyst yield.
And (4) comparing the results:
group one: 6kg of excrement of 26-29 days old and 11.3 hundred million oocysts in total yield, 7.8 hundred million oocysts are extracted, and the yield is 69%;
and a second group: the quantity of the feces of 26-29 days old is 7.5kg, the total output is 11.5 hundred million oocysts, 7.1 hundred million oocysts are extracted, and the yield is 62 percent;
and (3) group III: 9kg of excrement of 26-29 days old and 11.6 hundred million oocysts in total yield, 6.8 hundred million oocysts are extracted, and the yield is 59%;
group four: the quantity of the feces of 26-29 days old is 35kg, the total yield is 10.8 hundred million oocysts, 5.0 hundred million oocysts are extracted, and the yield is 46 percent.
And (4) analyzing results: the total yield of oocysts of four test groups is not great, but the feces yield of the chickens of 26-29 days old fed with the composition 1 is reduced by 20 percent and 33 percent respectively compared with the chickens fed with the compositions 2 and 3, and the recovery rate of oocysts is 7 percent and 10 percent higher. The amount of feces in the chickens 26-29 days old fed with the composition 1 is reduced by 83 percent compared with that in the chickens fed with the normal feed, and the recovery rate of oocysts is 23 percent higher. The data show that the composition can reduce the discharge amount of excrement, improve the yield of oocysts obtained by extracting the eimeria maxima oocysts from the excrement, greatly improve the extraction efficiency of the eimeria maxima oocysts, reduce the expenditure of manpower, material resources and energy consumption and reduce the production cost.
Claims (3)
1. The application of the composition in improving the extraction rate of the oocysts of the eimeria maxima is characterized in that: the composition comprises the following components in parts by weight:
30-65 parts of glucose;
25-40 parts of egg yolk powder;
0.2-3 parts of vitamin A;
0.1-2 parts of vitamin E;
vitamin D30.4-5 parts.
2. The use of a composition of claim 1 for increasing the oocyst extraction of eimeria maxima, wherein: the composition comprises the following components in parts by weight:
60 parts of glucose;
35 parts of egg yolk powder;
2 parts of vitamin A;
1 part of vitamin E;
vitamin D3 And 3 parts.
3. The use of the composition of claim 1 for increasing the oocyst extraction rate of eimeria maxima, wherein the method of preparing the composition comprises the steps of:
(1) respectively weighing vitamin A, vitamin E and vitamin D according to the weight ratio3Adding the mixture into a mixing tank, and premixing for 10 minutes for later use;
(2) weighing glucose according to the weight ratio, and putting the glucose into a mixing tank filled with the premix to mix for 20 minutes for later use;
(3) weighing yolk powder according to the weight ratio, and putting the yolk powder into a mixing tank filled with the premix to mix for 30 minutes; mixing, packaging into aluminum foil bags, and sealing; thus obtaining the composition for improving the extraction rate of the oocysts of the eimeria maxima.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711494600.8A CN107912643B (en) | 2017-12-31 | 2017-12-31 | Composition for improving extraction rate of oocysts of eimeria maxima and preparation method thereof |
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| CN201711494600.8A CN107912643B (en) | 2017-12-31 | 2017-12-31 | Composition for improving extraction rate of oocysts of eimeria maxima and preparation method thereof |
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| CN107912643B true CN107912643B (en) | 2021-04-20 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003009700A1 (en) * | 2001-07-20 | 2003-02-06 | Dsm Ip Assets B.V. | Coccidiostats |
| CN107019111A (en) * | 2016-01-31 | 2017-08-08 | 天津赫莱恩特生物科技有限公司 | Improve composition of huge eimeria tenella egg capsule yield and preparation method thereof |
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2017
- 2017-12-31 CN CN201711494600.8A patent/CN107912643B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003009700A1 (en) * | 2001-07-20 | 2003-02-06 | Dsm Ip Assets B.V. | Coccidiostats |
| CN107019111A (en) * | 2016-01-31 | 2017-08-08 | 天津赫莱恩特生物科技有限公司 | Improve composition of huge eimeria tenella egg capsule yield and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| "球虫疫苗稀释剂的制备及其对疫苗免疫效果的影响"，朱晓波，中国优秀硕士学位论文全文数据库 农业科技辑，第2期，D050-341，2015年2月15日;朱晓波;《中国优秀硕士学位论文全文数据库 农业科技辑》;20150215(第2期);第13页倒数第3行-第14页第2行 * |
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