CN107007763A - A kind of Chinese medicine composition for being used to prevent sheldrake coli-infection disease - Google Patents
A kind of Chinese medicine composition for being used to prevent sheldrake coli-infection disease Download PDFInfo
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- CN107007763A CN107007763A CN201710336101.XA CN201710336101A CN107007763A CN 107007763 A CN107007763 A CN 107007763A CN 201710336101 A CN201710336101 A CN 201710336101A CN 107007763 A CN107007763 A CN 107007763A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8984—Dendrobium
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/62—Nymphaeaceae (Water-lily family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The present invention relates to a kind of Chinese medicine composition for being used to prevent sheldrake coli-infection disease, by mulberry leaf, lotus leaf and Jenkine Dendrobium according to weight ratio 15:1‑3:1 composition.Described Chinese medicine composition is prepared into aqua, and (1) shreds mulberry leaf, lotus leaf and Jenkine Dendrobium, is 15 according to weight ratio:1‑3:1 is mixed to get mixture；(2) in the mixture according to mixture and water weight ratio be 1:15 20 add water, and it is 80 100 DEG C to control water temperature, and filtrate is collected after extracting 1 2h；(3) repeat step (2) is extracted once again；(4) combining step (2) and step (3) gained filtrate twice, that is, obtain described aqua.The application that above-mentioned aqua is mixed with other feeding material and feeds sheldrake is also claimed in the present invention.Proved through experimental study, mulberry leaf, lotus leaf and Jenkine Dendrobium are used in combination with specific part by weight, synergy is obvious, and evident in efficacy, cost is low.
Description
Technical field
The invention belongs to sheldrake breeding technology field, more particularly to a kind of it is used to prevent sheldrake coli-infection disease
Chinese medicine composition, for treating sheldrake disease as caused by coli-infection.
Background technology
Sheldrake colibacillosis can fall ill throughout the year, be the most common bacterial disease of sheldrake, can infect all age brackets
Sheldrake.Often cause the diseases such as septicemia, air bag inflammation, peritonitis, salpingitis, arthritis, pericarditis.
Duck source Escherichia coli mainly the approach such as contact with each other by feeding material, drinking-water, ground, air, animal body and passed
Broadcast, belong to conditionity pathogenic bacteria.Current subject matter has：There is the phenomenon of abuse of antibiotics in considerable plant, promotes big
The generation of enterobacteria drug resistance.Escherichia coli are typically taken in by food chain, not only pathogenic drug-fast bacteria can be passed into the mankind, and
And the drug resistant gene of endogenous resistance flora can be diffused into environment and together pass to human disease bacterium, produced with antimicrobial short of money
Anti- effect, the life security of the serious threat mankind.In addition comprehensive preventive health measures imperfection, feeding and management level is limited, prevention meaning
It is that the generation of disease and propagation provide condition to know thin grade.
At present, people begin look for the drug resistance that some Chinese herbal medicines are used to reverse antibiotic Western medicine, or attempt to filter out
It directly can prevent or suppress the Chinese herbal medicine of ETEC.
Mulberry leaf, are the leaf of Moraceae category.Mulberry plant has hypoglycemic, hypotensive, antibacterial and a variety of biologies such as antiviral
Activity.There are some researches show, mulberry leaf water extract and ethanol extract have to bacterium such as staphylococcus aureus, Escherichia coli etc. compared with
Good inhibitory action.
Lotus leaf is the leaf of Nymphaeceae nelumbium lotus, with clearing summer-heat and damp, sending up the lucid yang, clears away heart-fire and reduces phlegm and internal heat, stops blooding Li Shui's
Effect.It is reported that containing a variety of alkaloids and flavone compound in lotus leaf, wherein the alkali acted on strong antimitotic
Property composition-alkaloid is one of main active of lotus leaf.Alkaloid, which has, in lotus leaf promotees bacterium and antibacterial double action, and has
The effects such as antitumor, analgesia, anesthesia.There is experiment to show, metabolism of the lotus leaf alkaloid liquid to Escherichia coli is inhibited, with
The increase growth rate constant linear reduction of drug concentration, the optimal medication that lotus leaf alkaloid liquid is acted on Metabolism of E. coli is dense
Spend for 0.01146mg/mL.
Jenkine Dendrobium, golden yellow damp, the upper tree shrimp of alias, upper Shu Huangze, the chicken back of the body stem of noble dendrobium, shrimp public affairs are careless, small ring grass.Jenkine Dendrobium is orchid family
The herb of the poly- stem of noble dendrobium of plant, is distributed mainly on the ground such as Guangdong, Hainan, Guangxi, Yunnan.Slightly sweet flavor, it is cool in nature.Attach to the lung and stomach meridians.Have
Moisten the lung and relieve the cough, effect of tonifying-Yin and nourishing-stomach.Treat cough with lung heat, pulmonary tuberculosis, asthma, dysentery, stomatitis, stomachache etc. illness.
Through retrieval, do not inquire to combine mulberry leaf, lotus leaf and Jenkine Dendrobium and drawn for treating sheldrake by coli-infection
The relevant report of the disease risen.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of Chinese medicine group for being used to prevent sheldrake coli-infection disease
Compound, it acts synergistically obvious, it is not necessary to reuse the purpose that prevention and control coli-infection disease can be achieved in antibiotic.
To solve above technical problem, the present invention uses following technical scheme：One kind is used to prevent sheldrake Escherichia coli sense
The Chinese medicine composition of disease is contaminated, is made up of mulberry leaf, lotus leaf and Jenkine Dendrobium, the weight ratio of mulberry leaf, lotus leaf and Jenkine Dendrobium is 1-5:1-
3:1。
Described Chinese medicine composition is prepared into aqua, and preparation method is as follows：
(1) mulberry leaf, lotus leaf and Jenkine Dendrobium are shredded, is 1-5 according to weight ratio:1-3:1 is mixed to get mixture；
(2) in the mixture according to mixture and water weight ratio be 1:15-20 adds water, and it is 80-100 to control water temperature
DEG C, collect filtrate after extracting 1-2h；
(3) repeat step (2) is extracted once again；
(4) combining step (2) and step (3) gained filtrate twice, that is, obtain described aqua.
The application that above-mentioned aqua is mixed with other feeding material and feeds sheldrake is also claimed in the present invention.
The beneficial effect that the present invention possesses is：Proved through experimental study, mulberry leaf, lotus leaf and Jenkine Dendrobium are with specific weight ratio
Example is used in combination, and synergy is obvious, and evident in efficacy, cost is low.
Embodiment
Technical scheme and beneficial effect are described further with reference to specific embodiment, this embodiment
The Jenkine Dendrobium used is the blade and spray of Jenkine Dendrobium, and following part by weight is fresh weight ratio.
First, the measure of mulberry leaf, lotus leaf and Jenkine Dendrobium to Escherichia coli synergy
1. test medicine：
(1) test medicine formula table
(2) method that medicament is prepared into aqua：
1) mulberry leaf, lotus leaf and Jenkine Dendrobium are shredded, takes each Chinese medicine to be mixed according to formula shown in above-mentioned formula table, obtain
It is formulated 1#-20#；Formula table is that " 0 " represents that this kind of Chinese medicine does not take.
2) it is formulated No. 1#-19# and respectively weighs 200g, be separately added into 4kg water, it is 80-100 DEG C to control water temperature, is extracted after 2h
Collect filtrate；Formula 20# only adds 4kg water, controls water temperature to be boiled for 80-100 DEG C and remaining water is poured out after 2h；
3) repeat step 2) extract or boiled again once again；
4) combining step 2) and step 3) gained filtrate or water twice, that is, obtain aqua 1#-20#.
2. subjects：The Escherichia coli of sheldrake infection.
3. test method：
MIC of each aqua to Escherichia coli is determined using liquid coubling dilution.
Above-mentioned 20 kinds of aquas, every kind of aqua takes 7 test tubes respectively, numbering 1-7, and every loads 1ml broth bouillons.
The aqua 1#-20# that water extraction is obtained is considered as 100% mother liquor；Above-mentioned aqua is separately added into each No. 1 test tube so that each aqua
Initial concentration be 1000mg/ml；After No. 1 test tube is well mixed, 1ml mixed liquors are taken to No. 2 test tubes, by that analogy to No. 7
Test tube is drawn 1ml mixed liquors and cast out, and completes doubling dilution.
It is 10 to add bacteria containing amount in the cultured escherichia coli suspensions of 0.1ml, suspension to every test tube5CFU/ml.Will be complete
Portion's test tube is placed in 37 DEG C, cultivates 24h on 120r/min shaking table, and the least concentration for not occurring muddiness is the aqua to large intestine bar
The MIC of bacterium.
4. result of the test
MIC measurement result (concentration unit of each aqua to Escherichia coli：mg/mL)
Note："+" represents that long bacterium is muddy in upper table；"-" represents that not long bacterium does not occur muddiness.
As seen from the above table, 20# is that 7 test tubes of blank control are all that long bacterium is muddy.
17# is that single mulberry leaf are 500mg/ml to the MIC of Escherichia coli, and 18# is that single lotus leaf is to the MIC of Escherichia coli
500mg/ml, 19# are that single Jenkine Dendrobium is 1000mg/ml to the MIC of Escherichia coli.
15# is the mixing aqua of mulberry leaf and Jenkine Dendrobium, and its MIC is 500mg/ml, and 16# is the mixing water of lotus leaf and Jenkine Dendrobium
Agent, its MIC is 500mg/ml；15# and 16# show obvious synergy compared with single dose, not.
The mixing aqua of 14# mulberry leaf and lotus leaf, its MIC is 1000mg/ml, compared with single dose, shows antagonism.
4#, 5#, 7#, 8#, 10#, 12# MIC are 31.25mg/ml；Compared with single dose, obvious synergy is shown.
1#, 3#, 11#, 13# MIC are 500mg/ml, compared with single dose, do not show obvious synergy.
The MIC that 2# MIC is more than 1000mg/ml, 6# and 9# is 1000mg/ml, compared with single dose, shows antagonism work
With.
2nd, the experiment in vitro that mulberry leaf, lotus leaf and Jenkine Dendrobium are used in combination
Test group：8# aquas
Control group 1-3：17#, 18#, 19# aqua.
Subjects：Make a definite diagnosis the sheldrake group for having infected E. coli isolated from ducks disease.
Test method：Morbidity duck group is divided into 4 groups, every group respectively according to per kilogram coarse food grain feeding material addition 300g aquas
8#, 17#, 18#, 19# are fed；The coarse food grain feeding material is corn flour, the mixture of green vegetables；Each group is persistently fed 7 days.
Result of the test：The sick duck feed intake of test group increased, no death, all be fully recovered using duck group after 7 days；Control
The group 1-3 most of still wans of duck group, the sick duck death rate is respectively 3%, 5%, 3%.
Claims (3)
1. a kind of Chinese medicine composition for being used to prevent sheldrake coli-infection disease, it is characterised in that by mulberry leaf, lotus leaf and wood
Shrimp public affairs composition, the weight ratio of mulberry leaf, lotus leaf and Jenkine Dendrobium is 1-5:1-3:1.
2. the Chinese medicine composition according to claim 1 for being used to prevent sheldrake coli-infection disease, it is characterised in that
Described Chinese medicine composition is prepared into aqua, and preparation method is as follows：
(1) mulberry leaf, lotus leaf and Jenkine Dendrobium are shredded, is 1-5 according to weight ratio:1-3:1 is mixed to get mixture；
(2) in the mixture according to mixture and water weight ratio be 1:15-20 adds water, and it is 80-100 DEG C to control water temperature, is carried
Take and filtrate is collected after 1-2h；
(3) repeat step (2) is extracted once again；
(4) combining step (2) and step (3) gained filtrate twice, that is, obtain described aqua.
3. the aqua described in claim 2 mixes the application fed with other feeding material.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710336101.XA CN107007763A (en) | 2017-05-12 | 2017-05-12 | A kind of Chinese medicine composition for being used to prevent sheldrake coli-infection disease |
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| CN201710336101.XA CN107007763A (en) | 2017-05-12 | 2017-05-12 | A kind of Chinese medicine composition for being used to prevent sheldrake coli-infection disease |
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| CN107007763A true CN107007763A (en) | 2017-08-04 |
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| CN201710336101.XA Pending CN107007763A (en) | 2017-05-12 | 2017-05-12 | A kind of Chinese medicine composition for being used to prevent sheldrake coli-infection disease |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107568495A (en) * | 2017-10-24 | 2018-01-12 | 天峨县平昌生态农业有限公司 | A kind of chicken feed addictive |
-
2017
- 2017-05-12 CN CN201710336101.XA patent/CN107007763A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 张兰英: "《荷叶生物碱的提取及体外抑菌和凝血活性研究》", 《特产研究》 * |
| 梁薇: "《桑叶水提物及醇提物抗菌作用的研究》", 《时珍国药》 * |
| 江苏省植物研究所: "《新华本草纲要 第三册》", 31 December 1990 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107568495A (en) * | 2017-10-24 | 2018-01-12 | 天峨县平昌生态农业有限公司 | A kind of chicken feed addictive |
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Application publication date: 20170804 |