CN107006542A - A kind of preparation method for the plant extracts for preventing fruit russeting to produce - Google Patents
A kind of preparation method for the plant extracts for preventing fruit russeting to produce Download PDFInfo
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- CN107006542A CN107006542A CN201710398069.8A CN201710398069A CN107006542A CN 107006542 A CN107006542 A CN 107006542A CN 201710398069 A CN201710398069 A CN 201710398069A CN 107006542 A CN107006542 A CN 107006542A
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- plant extracts
- fruit russeting
- russeting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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- General Health & Medical Sciences (AREA)
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- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
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Abstract
The present invention relates to a kind of preparation method of prevention fruit russeting plant extracts, comprise the following steps：1) Bark of Eucommia Ulmoides or cauline leaf low temperature drying, alcohol extracting；2) sweet potato leaves and stemses are dried in the shade, and hot water is extracted；3) blend step 1 in proportion) and step 2) solution, pH value is adjusted, is settled, filtering；4) step 3) filtrate passes through strong base anion resin adsorption；5) low-concentration sulfuric acid solution stripping resin obtains stripping liquid；5) stripping liquid hydrogenation potassium oxide readjustment pH, compounding chitosan oligosaccharide, polylysine, aspartic acid, glycerine, sedimentation obtains supernatant；6) stigmasterol solution is continuously added, is stirred, it is filling to produce product.This technique extracts active material from the bark of eucommia and sweet potato leaves and stemses, enriching and purifying prepares product, in thank spend after can effectively prevent the generation of apple, grape, pears, fig, Kiwi berry, winter jujube, citrus fruit russeting to young fruit period foliage-spray, strengthen fruit surface finish and brightness, reduce pericarp asterism.
Description
Technical field
The present invention relates to a kind of preparation method of the plant extracts of prevention fruit russeting, belong to technical field of fertilizer production.
Background technology
Run into pest and disease damage during fruit surface cell is exposed, wind and rain and sunshine are excessively or the environmental factor such as applying pesticides
Effect, can form phellem layer on young fruit and then generate cork cell, ultimately form fruit russeting, regional such a situation of high humidity is outstanding
To be serious.Though fruit russeting does not influence edible quality, exterior quality is had a strong impact on, so as to reduce the commodity value of fruit.In recent years
As people improve to eating fruit quality requirement raw, effectively solve the problems, such as that fruit russeting turns into planting fruit trees industry study hotspot.
Prevent rust from being more common method using bactericide and antibiotic.Patent CN201610506314.8,
CN201410445438.0, CN201410233649.8, CN201610506331.1 are directed to use with ZJ0712, pyrazoles indanyl
The material such as formamide, Tebuconazole, metalaxyl, carbendazim, Mancozeb, phenyl ether methyl cyclic file, five sodium chlorate, Enestroburin kills disease
Bacterium, prevention rust is produced；Penicillin or terramycin solution are applied under the bark shelled by patent CN201310091993.3, are carried
High usage；Tetramycin and Xinjunan acetate compounding are strengthened bactericidal effect by patent CN201310636219.6.Bactericide and
Antibiotic causes rust effect to fungal disease preferably, to other types fruit russeting without prevention effect.
Bagging and biomembrane can effectively prevent fruit surface damage, there is certain prevention effect to fruit russeting.Patent
CN200520034028.3 discloses a kind of novel fruit bag waterproof ventilated membrane, and 30~300 μm of micropores, phase are made a call on macromolecule membrane
The pitch of holes of adjacent micropore is less than 2300 μm, keeps fruit bag internal and external temperature, wetting balance.Patent CN201410159153.0 is disclosed
A kind of method for reducing the operatic circle rust, comprises the following steps：15-25d sets printing opacity crosses the anti-water monolayer pouch of wax after fallen flowers；40-50d exists
Pouch overcoat is light tight to cross wax waterproof dual-layer sack.Patent CN91108817.2 utilizes pertroleum wax, dispersant, modified resin, solidifying
Emulsion is made in gelatinizing agent, compound emulsifying agent in proportion, is sprayed on fruit face and forms biomembrane.Bagging or film coating technique are to fruit table
Skin physical damnification has preferable preventive and therapeutic effect, but profit inconvenient for use, and cost of labor is higher.
Application of the conditioning agent in terms of fruit russeting is prevented and treated.Patent CN201410173000.1 discloses apple full-bloom stage and fallen flowers
Phase sprays the method that Gibberellins solution reduces apple fruit russeting, is shared with bagging, effect is obvious.But gibberellin concentration has strictly
Limitation, improper use easily triggers poisoning.
Fruit post-processing can also eliminate part fruit russeting.Patent CN201410161918.4 discloses a kind of removing macaque
The method of peach fruit russeting, oxalic acid and citric acid are configured to soak 10-20min in mixed solution, Kiwi berry solution, dried afterwards,
Remove fruit russeting effect obvious.Patent CN200610042851.8 is using oxalic acid and sodium chloride solution to the hairy fruit of surface band
Carry out fruit russeting cleaning.Organic acid treatment also has the effect of extension storage phase, but is only applicable to rough fruit.
The content of the invention
It is contemplated that a kind of prevention fruit russeting of design produces plant extracts preparation method, it is characterised in that including as follows
Step：
1) Bark of Eucommia Ulmoides or cauline leaf are dried under the conditions of being less than 50 DEG C, are added the 10 times of ethanol of volume 95% immersion 1h, are controlled temperature
45 DEG C -55 DEG C, 2h is incubated, extract solution 1 is filtered to obtain while hot；
2) spontaneously dried under the conditions of sweet potato leaves and stemses lucifuge, add 5-10 times of volume of water, control 80 DEG C -95 DEG C of temperature, stirring
2h, filters to obtain extract solution 2；
3) 1 is pressed:5 ratio mixed extracts 1 and extract solution 2, added with machine acid for adjusting pH value 3.0-5.0, room temperature natural subsidence,
Plate-frame filtering obtains clear liquid；
4) step 3) filtrate adsorbed with 4 times of column volumes/hour flow velocity by strongly basic anionic resin, and peritoneal effluent pH value is dashed forward
So reduction, stops absorption；
5) 0.1-0.2mol/L sulfuric acid solutions collect stripping liquid with 0.5 times of column volume/hour flow velocity desorption resin；
6) stripping liquid hydrogenation potassium oxide readjustment pH to 3.5-4.5, compounding chitosan oligosaccharide, polylysine, aspartic acid, the third three
Alcohol, sedimentation, is transferred to extractor, separates to obtain supernatant；
7) step 6) supernatant continuously adds 2%-3% stigmasterol solution, stirs, filling to produce product.
Further, step 1) Bark of Eucommia Ulmoides or cauline leaf drying standard be loss on drying be less than 15%.
Further, step 3) organic acid be glacial acetic acid, malic acid, the one or more of citric acid.
Further, step 4) the strong base anion resin be 201 resins one kind.
Further, step 4) wash 3h with 2mol/L potassium hydroxide solutions before using strong base anion resin.
Further, step 5) stripping liquid of first resin column volume do not collect.
Further, step 6) compounding chitosan oligosaccharide 1%-3%, polylysine 0.5%-0.8%, aspartic acid 1%-4%, third
Triol 2%-6%.
Further, step 7) stigmasterol solution is added for the saturated solution of stigmasterol in ethanol.
The present invention is also claimed the prevention fruit russeting prepared using the above method and produces plant extracts and its in apple
Really, the application in the kind such as grape, pears, fig, Kiwi berry, winter jujube, citrus.
The beneficial effects of the invention are as follows：
1) present invention is prepared by raw material using natural active matter, product effect stability and using safe.From plant extract
A large amount of low molecular organic acidses, aldehydes matter are obtained in thing can effectively prevent fruit cork cell from producing, and fruit russeting is reduced from source
Occurrence probability, applicable crops are extensive.
2) simple production process of the present invention, equipment requirement is low, it is easy to large-scale production.Ion exchange resin bioaccumulation efficiency
Height, can Reusability, reduce concentration power cost, be adapted to batch production.
3) raw material sources enrich.The bark of eucommia and sweet potato leaves and stemses are artificial growth gained, cheap and in liberal supply.
Embodiment
The principle and feature of the present invention are described below in conjunction with example, the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the present invention.
Embodiment 1
1) drying under the conditions of bark of eucommia cauline leaf 10kg is less than 50 DEG C is until loss on drying is less than 15%, 10 times of volumes 95% of addition
Ethanol soaks 1h, controls temperature 45 C, is incubated 2h, the volume 80L of extract solution 1 is filtered to obtain while hot；
2) spontaneously dried under the conditions of sweet potato leaves and stemses 50kg lucifuges, add 10 times of volume of water, control 80 DEG C of temperature, stir 2h,
Filter to obtain the volume 400L of extract solution 2；
3) 1 is pressed:5 ratio mixed extracts 1 and extract solution 2, vinegar acid for adjusting pH value 3.5 on the rocks, room temperature natural subsidence, sheet frame
Filter to obtain clear liquid 450L；
4) step 3) filtrate washs 3h with 2mol/L potassium hydroxide solutions, passed through afterwards with 4 times of column volumes/hour flow velocity
201 resins (degree of cross linking 12) are adsorbed, and peritoneal effluent pH value is reduced suddenly, stop absorption；
5) 0.1mol/L sulfuric acid solutions are with 0.5 times of column volume/hour flow velocity desorption resin, the stripping liquid of first column volume
It is discarded, collect remaining stripping liquid, volume 300L；
6) stripping liquid hydrogenation potassium oxide readjustment pH to 3.5, compounding chitosan oligosaccharide 3Kg, polylysine 1.5Kg, aspartic acid
5Kg, glycerine 6Kg, sedimentation, are transferred to extractor, separate to obtain supernatant；
7) step 6) supernatant continuously adds 9L stigmasterol saturated solutions, stirs, filling to produce product.
8) product is diluted into 200 times to spray in winter jujube crop, fruit expanding period to maturation is sprayed 3 times, be spaced 7 days, experiment
Group fruit russeting generation rate is substantially reduced, and winter jujube surface-brightening is smooth, healthy fruit increase.
Embodiment 2
1) drying under the conditions of bark of eucommia cauline leaf 20kg is less than 50 DEG C is until loss on drying is less than 15%, 10 times of volumes 95% of addition
Ethanol soak 1h, control temperature 50 C, be incubated 2h, filter while hot the volume of extract solution 1 be 170L；
2) spontaneously dried under the conditions of sweet potato leaves and stemses 100kg lucifuges, add 7 times of volume of water, control 80 DEG C of temperature, stir 2h,
Filter the volume of extract solution 2 be 650L；
3) 1 is pressed:5 ratio mixed extracts 1 and extract solution 2, adding citric acid regulation pH value 4.0, room temperature natural subsidence, sheet frame
Filter to obtain clear liquid 800L；
4) step 3) filtrate washs 3h with 2mol/L potassium hydroxide solutions, passed through afterwards with 4 times of column volumes/hour flow velocity
DM201 resins (degree of cross linking 20) are adsorbed, and peritoneal effluent pH value is reduced suddenly, stop absorption；
5) 0.2mol/L sulfuric acid solutions are with 0.5 times of column volume/hour flow velocity desorption resin, the stripping liquid of first column volume
It is discarded, remaining stripping liquid volume is collected for 500L；
6) stripping liquid hydrogenation potassium oxide readjustment pH to 4.0, compounding chitosan oligosaccharide 15Kg, polylysine 4Kg, aspartic acid 5Kg,
Glycerine 10Kg, sedimentation, is transferred to extractor, separates to obtain supernatant；
7) step 6) supernatant continuously adds 15L stigmasterol saturated solutions, stirs, filling to produce product.
8) product is diluted into 200 times to be sprayed in the fruit tree growth phase, is spaced 10 days, sprays altogether 4 times, it can be seen that experimental group fruit tree
Fruit russeting generation rate it is extremely low, pericarp surface-brightening is smooth, and colouring is uniform.
Embodiment 3
1) drying under the conditions of bark of eucommia cauline leaf 25kg is less than 50 DEG C is until loss on drying is less than 15%, 10 times of volumes 95% of addition
Ethanol soaks 1h, controls 48 DEG C of temperature, is incubated 2h, the volume 220L of extract solution 1 is filtered to obtain while hot；
2) spontaneously dried under the conditions of sweet potato leaves and stemses 100kg lucifuges, add 5 times of volume of water, control 80 DEG C of temperature, stir 2h,
Filter to obtain the volume 480L of extract solution 2；
3) 1 is pressed:5 ratio mixed extracts 1 and extract solution 2, acetic acid on the rocks, apple acid for adjusting pH value 4.5, room temperature are sunk naturally
Drop, plate-frame filtering obtains clear liquid 570L；
4) step 3) filtrate washs 3h with 2mol/L potassium hydroxide solutions, passed through afterwards with 4 times of column volumes/hour flow velocity
201 resins (degree of cross linking 16) are adsorbed, and peritoneal effluent pH value is reduced suddenly, stop absorption；
5) 0.1mol/L sulfuric acid solutions are with 0.5 times of column volume/hour flow velocity desorption resin, the stripping liquid of first column volume
It is discarded, collect remaining stripping liquid volume 350L；
6) stripping liquid hydrogenation potassium oxide readjustment pH to 4.5, compounding chitosan oligosaccharide 7Kg, polylysine 2Kg, aspartic acid 7Kg,
Glycerine 10Kg, sedimentation, is transferred to extractor, separates to obtain supernatant；
7) step 6) supernatant continuously adds 10L stigmasterol saturated solutions, stirs, filling to produce product.
8) product is diluted into 300 times to spray in grape fruit expanding stage, is spaced 8 days, sprays altogether 3 times, experimental group uva
Real surface is smooth, and fruit russeting generation rate is almost 0, and fruit is just and full, and mass color is good.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (9)
1. a kind of plant extracts preparation method for preventing fruit russeting to produce, it is characterised in that comprise the following steps：
1) Bark of Eucommia Ulmoides or cauline leaf are dried under the conditions of being less than 50 DEG C, the 10 times of ethanol of volume 95% immersion 1h of addition, and control temperature 45 C-
55 DEG C, 2h is incubated, extract solution 1 is filtered to obtain while hot；
2) spontaneously dried under the conditions of sweet potato leaves and stemses lucifuge, add 5-10 times of volume of water, control 80 DEG C -95 DEG C of temperature, stir 2h, mistake
Filter to obtain extract solution 2；
3) 1 is pressed:5 ratio mixed extracts 1 and extract solution 2, added with machine acid for adjusting pH value 3.0-5.0, room temperature natural subsidence, sheet frame
Filter to obtain clear liquid；
4) step 3) filtrate adsorbed with 4 times of column volumes/hour flow velocity by strongly basic anionic resin, and peritoneal effluent pH value drops suddenly
It is low, stop absorption；
5) 0.1-0.2mol/L sulfuric acid solutions collect stripping liquid with 0.5 times of column volume/hour flow velocity desorption resin；
6) stripping liquid hydrogenation potassium oxide readjustment pH to 3.5-4.5, compounding chitosan oligosaccharide, polylysine, aspartic acid, glycerine sink
Drop, is transferred to extractor, separates to obtain supernatant；
7) step 6) supernatant continuously adds 2%-3% stigmasterol solution, stirs, filling to produce product.
2. a kind of prevention fruit russeting according to claim 1 produces plant extracts preparation method, it is characterised in that step 1)
Bark of Eucommia Ulmoides or cauline leaf drying standard are that loss on drying is less than 15%.
3. a kind of prevention fruit russeting according to claim 1 produces plant extracts preparation method, it is characterised in that step 3)
The organic acid is glacial acetic acid, malic acid, the one or more of citric acid.
4. a kind of prevention fruit russeting according to claim 1 produces plant extracts preparation method, it is characterised in that step 4)
The strong base anion resin is one kind of 201 resins.
5. a kind of prevention fruit russeting according to claim 1 or 4 produces plant extracts preparation method, it is characterised in that step
It is rapid 4) to wash 3h with 2mol/L potassium hydroxide solutions before using strong base anion resin.
6. a kind of prevention fruit russeting according to claim 1 produces plant extracts preparation method, it is characterised in that step 5)
The stripping liquid of first resin column volume is not collected.
7. a kind of prevention fruit russeting according to claim 1 produces plant extracts preparation method, it is characterised in that step 6)
Compound chitosan oligosaccharide 1%-3%, polylysine 0.5%-0.8%, aspartic acid 1%-4%, glycerine 2%-6%.
8. a kind of prevention fruit russeting according to claim 1 produces plant extracts preparation method, it is characterised in that step 7)
It is the saturated solution of stigmasterol in ethanol to add stigmasterol solution.
9. the prevention fruit russeting that a kind of preparation method according to any one of claim 1-8 is prepared produces plant extract
Thing and its application in the kinds such as apple, grape, pears, fig, Kiwi berry, winter jujube, citrus.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710398069.8A CN107006542A (en) | 2017-05-31 | 2017-05-31 | A kind of preparation method for the plant extracts for preventing fruit russeting to produce |
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| CN201710398069.8A CN107006542A (en) | 2017-05-31 | 2017-05-31 | A kind of preparation method for the plant extracts for preventing fruit russeting to produce |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107548850A (en) * | 2017-09-26 | 2018-01-09 | 界首市方辉家庭农场 | A kind of implantation methods for improving pear and date-printing blocks percentage of fertile fruit |
| CN114097545A (en) * | 2021-12-16 | 2022-03-01 | 广西壮族自治区农业科学院 | Rust-free cultivation method for fruit of banana |
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| CN102860225A (en) * | 2012-10-10 | 2013-01-09 | 南京农业大学 | Method for reducing fruit russeting of golden delicious apples |
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2017
- 2017-05-31 CN CN201710398069.8A patent/CN107006542A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860225A (en) * | 2012-10-10 | 2013-01-09 | 南京农业大学 | Method for reducing fruit russeting of golden delicious apples |
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| 余霜: "《农业经济发展实证分析》", 31 August 2015, 中国农业出版社 * |
| 孙玉琳: "《蔬菜.瓜类.果树 病虫草害防治新技术》", 31 August 1993, 河南科学技术出版社 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107548850A (en) * | 2017-09-26 | 2018-01-09 | 界首市方辉家庭农场 | A kind of implantation methods for improving pear and date-printing blocks percentage of fertile fruit |
| CN114097545A (en) * | 2021-12-16 | 2022-03-01 | 广西壮族自治区农业科学院 | Rust-free cultivation method for fruit of banana |
| CN114097545B (en) * | 2021-12-16 | 2022-10-18 | 广西壮族自治区农业科学院 | Rust-free cultivation method for fruit of banana |
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