CN106983901A - 一种载药缓释创面敷料的制备方法 - Google Patents
一种载药缓释创面敷料的制备方法 Download PDFInfo
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Abstract
本发明提供一种载药缓释创面敷料的制备方法，通过静电纺丝法，对纺丝溶液在纺丝条件下进行纺丝，获得所述载药缓释创面敷料；其中，所述纺丝溶液为含有载药纳米颗粒及聚乳酸‑羟基乙酸共聚物的四氢呋喃与N,N‑二甲基甲酰胺的混合液；所述纺丝条件为温度25～30℃、相对湿度为25％～40％、电压11～13kV、流速0.9～1.3ml/L。在本发明中，以静电纺丝技术为基础，提供了一种简单易行，适合工业化的载药缓释创面敷料制备方法。
Description
技术领域
本发明涉及药物缓释领域，特别涉及一种载药缓释创面敷料的制备方法。
背景技术
对于载药的创面敷料而言，为了更好的提高药物利用率，需对载药的释放速率进行控制。
传统载药创面敷料为简单混合法，即，将载药与创面敷料材料进行简单混合。这种方法存在药物突释行为的问题，即，载药的释放速率突然增大，这就导致了创面上的药物浓度急剧上升，对患者产生一定的毒副影响，而后期药量又无法满足创面愈合的要求，直接影响创面的愈合效率。
因此，我们需要一种新的创面敷料的制备方法，以解决目前技术上的空白。
发明内容
本发明的目的在于提供一种载药缓释创面敷料的制备方法，以静电纺丝技术为基础，提供了一种简单易行，适合工业化的载药缓释创面敷料制备方法。
为了实现上述目的，本发明提供一种载药缓释创面敷料的制备方法，所述方法是通过静电纺丝法，对纺丝溶液在纺丝条件下进行纺丝，获得所述载药缓释创面敷料；其中，所述纺丝溶液为含有载药纳米颗粒及聚乳酸-羟基乙酸共聚物的四氢呋喃与N,N-二甲基甲酰胺的混合液；所述纺丝条件为温度25～30℃、相对湿度为25％～40％、电压11～13kV、流速0.9～1.3ml/L。
在本发明一实施例中，所述纺丝溶液中，所述四氢呋喃的浓度为0.886～0.889g/ml，所述N,N-二甲基甲酰胺的浓度为0.945～0.950g/ml，所述载药纳米颗粒与所述聚乳酸-羟基乙酸共聚物的质量比为1:1。
在本发明一实施例中，所述方法包括以下步骤：
(1)将抗菌药物或表皮生长因子与多孔性二氧化硅纳米粒子加入无水乙醇中混合，磁力搅拌后再超声搅拌至均匀，离心后干燥，获得载药纳米颗粒；
(2)将步骤(1)获得的载药纳米颗粒与聚乳酸-羟基乙酸共聚物加入四氢呋喃与N,N-二甲基甲酰胺的混合液中，磁力搅拌至均匀，获得纺丝溶液；
(3)对步骤(2)获得的纺丝溶液通过静电纺丝法进行纺丝，获得所述载药缓释创面敷料；其中，
在所述步骤(1)中，所述载药纳米颗粒的包封率为30～40％；
在所述步骤(2)中，载药纳米颗粒与聚乳酸-羟基乙酸共聚物的质量比为1:1，所述四氢呋喃的浓度为0.886～0.889g/ml，所述N,N-二甲基甲酰胺的浓度为0.945～0.950g/ml。
在本发明一实施例中，在所述步骤(1)中，磁力搅拌24小时，超声搅拌5～15分钟。
在本发明一实施例中，在所述步骤(1)中，在压力-1BAR、温度30℃下进行干燥。
在本发明一实施例中，所述聚乳酸-羟基乙酸共聚物的分子量为7万，所述无水乙醇的密度为0.789～0.791g/ml。
在本发明一较优实施例中，提供一种载药缓释创面敷料的制备方法，所述方法包括以下步骤：
(1)将抗菌药物或表皮生长因子与多孔性二氧化硅纳米粒子加入无水乙醇中混合，磁力搅拌24小时后再超声搅拌5～15分钟至均匀，离心后真空30℃干燥，获得载药纳米颗粒；其中，所述载药纳米颗粒的包封率为30～40％；
(2)将步骤(1)获得的载药纳米颗粒与聚乳酸-羟基乙酸共聚物一起加入四氢呋喃与N,N-二甲基甲酰胺的混合液中，磁力搅拌至均匀，获得纺丝溶液；其中，所述载药纳米颗粒与所述聚乳酸-羟基乙酸共聚物的质量比为1:1，所述四氢呋喃的浓度为0.886～0.889g/ml，所述N,N-二甲基甲酰胺的浓度为0.945～0.950g/ml；以及，
(3)对步骤(2)获得的纺丝溶液通过静电纺丝法进行纺丝，获得所述载药缓释创面敷料，所述纺丝条件为温度25～30℃、相对湿度为25％～40％、电压11～13kV、流速0.9～1.3ml/L。
在本发明一优选实施例中，以常规静电纺丝设备进行纺丝，静电纺丝针头选用16号针头，内径为1.32mm。
在本发明一实施例中，所述抗菌药物为穿心莲内脂。
需要说明的是，如无特殊要求，本发明中使用的试剂均为市售试剂。
在本发明中，结合载药纳米颗粒和静电纺丝技术，提供了一种简单易行且适合工业化的载药缓释创面敷料制备方法。本发明的所述制备方法具有步骤简单、方便快捷、易于产业化等优点，制备而得的载药缓释创面敷料具有良好的生物相容性和抗菌性，可以有效延缓药物的释放速率，提高药效。
具体实施方式
以下，结合具体实施方式，对本发明的技术进行详细描述。应当知道的是，以下具体实施方式仅用于帮助本领域技术人员理解本发明，而非对本发明的限制。
在本实施例中，提供一种载药缓释创面敷料的制备方法，所述方法包括以下步骤：
(1)将穿心莲内酯与多孔性二氧化硅纳米粒子加入无水乙醇中混合，磁力搅拌24小时后再超声搅拌5～15分钟至均匀，离心后真空30℃干燥，获得载药纳米颗粒；其中，所述穿心莲内酯与多孔性二氧化硅纳米粒子的质量比满足使得所述载药纳米颗粒的包封率为30～40％；
(2)将步骤(1)获得的载药纳米颗粒与聚乳酸-羟基乙酸共聚物一起加入四氢呋喃与N,N-二甲基甲酰胺的混合液中，磁力搅拌至均匀，获得纺丝溶液；其中，所述载药纳米颗粒与所述聚乳酸-羟基乙酸共聚物的质量比为1:1，所述四氢呋喃的浓度为0.886～0.889g/ml，所述N,N-二甲基甲酰胺的浓度为0.945～0.950g/ml；以及，
(3)对步骤(2)获得的纺丝溶液通过静电纺丝法进行纺丝，获得所述载药缓释创面敷料，所述纺丝条件为温度25～30℃、相对湿度为25％～40％、电压11～13kV、流速0.9～1.3ml/L，其中，以常规静电纺丝设备进行纺丝，静电纺丝针头选用16号针头，内径为1.32mm。
在本发明中，结合载药纳米颗粒和静电纺丝技术，提供了一种简单易行且适合工业化的载药缓释创面敷料制备方法。本发明的所述制备方法具有步骤简单、方便快捷、易于产业化等优点，制备而得的载药缓释创面敷料具有良好的生物相容性和抗菌性，可以有效延缓药物的释放速率，提高药效。
本发明已由上述相关实施例加以描述，然而上述实施例仅为实施本发明的范例。必需指出的是，已公开的实施例并未限制本发明的范围。相反地，包含于权利要求书的精神及范围的修改及均等设置均包括于本发明的范围内。
Claims (5)
1.一种载药缓释创面敷料的制备方法，所述方法是通过静电纺丝法，对纺丝溶液在纺丝条件下进行纺丝，获得所述载药缓释创面敷料；其中，
所述纺丝溶液为含有载药纳米颗粒及聚乳酸-羟基乙酸共聚物的四氢呋喃与N,N-二甲基甲酰胺的混合液；
所述纺丝条件为温度25～30℃、相对湿度为25％～40％、电压11～13kV、流速0.9～1.3ml/L。
2.如权利要求1所述的制备方法，其特征在于，所述纺丝溶液中，所述四氢呋喃的浓度为0.886～0.889g/ml，所述N,N-二甲基甲酰胺的浓度为0.945～0.950g/ml，所述载药纳米颗粒与所述聚乳酸-羟基乙酸共聚物的质量比为1:1。
3.如权利要求1所述的制备方法，其特征在于，所述方法包括以下步骤：
(1)将抗菌药物或表皮生长因子与多孔性二氧化硅纳米粒子加入无水乙醇中混合，磁力搅拌后再超声搅拌至均匀，离心后干燥，获得载药纳米颗粒；
(2)将步骤(1)获得的载药纳米颗粒与聚乳酸-羟基乙酸共聚物加入四氢呋喃与N,N-二甲基甲酰胺的混合液中，磁力搅拌至均匀，获得纺丝溶液；
(3)对步骤(2)获得的纺丝溶液通过静电纺丝法进行纺丝，获得所述载药缓释创面敷料；其中，在所述步骤(1)中，所述载药纳米颗粒的包封率为30～40％；
在所述步骤(2)中，载药纳米颗粒与聚乳酸-羟基乙酸共聚物的质量比为1:1，所述四氢呋喃的浓度为0.886～0.889g/ml，所述N,N-二甲基甲酰胺的浓度为0.945～0.950g/ml。
4.如权利要求3所述的制备方法，其特征在于，在所述步骤(1)中，磁力搅拌24小时，超声搅拌5～15分钟。
5.如权利要求3所述的制备方法，其特征在于，在所述步骤(1)中，在压力-1BAR、温度30℃下进行干燥。
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