CN106977292B - A kind of calcic water-soluble compound fertilizer and its preparation technology - Google Patents
A kind of calcic water-soluble compound fertilizer and its preparation technology Download PDFInfo
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- CN106977292B CN106977292B CN201710254558.6A CN201710254558A CN106977292B CN 106977292 B CN106977292 B CN 106977292B CN 201710254558 A CN201710254558 A CN 201710254558A CN 106977292 B CN106977292 B CN 106977292B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention belongs to technical field of fertilizers, more particularly to a kind of calcic water-soluble compound fertilizer and its preparation technology.The present invention is using dregs of beans as main component, it is aided with potato, Chinese yam, tea seed cake, molasses and sodium chloride prepare solid fermentation culture medium, pass through lactobacillus fermenti, bacillus subtilis, the method of more bacterium joint step fermentations such as Bacillus subtilis subspecies prepares plant active peptides, obtained plant active peptides and obtained polypeptide calcium powder good water solubility after calcium containing compound chelating, not easily runed off and by soil fixing with obtained calcic water-soluble compound fertilizer nutritional ingredient after a great number of elements raw material mixing granulation, utilization rate of fertilizer is high, be advantageous to increasing crop yield to increase income and the raising of crops quality.In addition, calcic water-soluble compound fertilizer provided by the invention is free of preservative, it is not susceptible to go mouldy, storage time length, meanwhile, it can effectively prevent and treat the soil-borne diseases such as root rot, powdery mildew, gray mold, reduce the incidence of soil-borne disease.
Description
Technical field
The invention belongs to technical field of fertilizers, and in particular to a kind of calcic water-soluble compound fertilizer and its preparation technology.
Background technology
Compound fertilizer refers to the chemical fertilizer containing two or more nutrient, and it has nutrient content height, accessory ingredient
The advantages that less and physical behavior is good, for balance fertilizing, improve utilization rate of fertilizer, promote crop stable high yield have it is very heavy
The effect wanted.Conventional compound fertilizer water solubility is slightly worse, is applied generally as base fertilizer, the main nitrogen supplemented needed for plant growth, phosphorus,
Potassium three elements.But crop growth not only needs N P and K, it is also necessary to middle trace element.Although crop is to moderate-element
Demand it is high not as good as N P and K, but once lack and will influence growing for crop, cause yield, quality etc. all under
Drop.
For calcium as one of 17 kinds of nutrients necessary to plant growth, the growth to plant has important influence.Calcium
The component and activator of some enzymes in the main constituents of plant soma wall and cell interbed, or plant are not only,
Metabolism to nitrogen and carbohydrate has a certain impact, meanwhile, there is facilitation to plant absorption potassium ion.Plant once lacks
Calcium will produce calcium deficiency symptom, trigger a variety of physiological diseases caused by calcium deficiency, during such as calcium deficiency the growth of plant can be pressed down
System, the tip of a root, stem end etc. can fester necrosis when serious；Fruit tree calcium deficiency will cause fruit development lopsided, induce and aggravate fruit physiology
Imbalance and the generation of disease, cause low fruit yields, poor quality and fruit resistance to storage period short etc..
Agricultural is replenished the calcium based on the inorganic calciums such as calcium hydroxide, calcium oxide in the market.Although inorganic calcium is cheap,
Its effect is poor, and plant is difficult to absorb, and chronic administration, easily causes soil pH rise.Most of inorganic calcium is insoluble matter, conventional multiple
Hefei material can not precipitate with calcium with applying, cause the fixation of phosphorus, calcium in soil, drop because sulfate, phosphate can generate with calcium
Low utilization rate of fertilizer.And although EDTA-Ca can be administered simultaneously with a great number of elements, cost is higher.
Peptide is a-amino acid with the compound that peptide chain links together and is formed, and is the intermediate product of protein hydrolysis, leads to
The compound being made up of 10~100 amino acid is often called polypeptide.Polypeptide has two kinds of biologically active peptide and artificial synthetic polypeptide, raw
Thing active peptide is mainly obtained by protein through biology extraction, has very strong activity.In agriculture application aspect, polypeptide not only can be with
Organic nutritional ingredient is provided for crop, physiological function regulation, cell tissue activation for crop, crop anti-adversity can improve
Deng also having a significant effect.In addition, polypeptide also has sequestering power, the chela of generation good water solubility can be chelated with moderate-element
Polymerisable compounds are applied in fertilizer.
The A of Chinese patent application CN 105272478 disclose a kind of protein peptides water-soluble fertilizer, and dregs of beans adds enzyme preparation and carried out
Hydrolysis obtains Soyprotein peptide, and Soyprotein peptide is after 50~60 DEG C carry out chelatropic reaction with moderate-element salt, reaction product
One or more after concentrated and in nitrogenous fertilizer, phosphate fertilizer, potash fertilizer continue reaction and obtain protein peptides water-soluble fertilizer.The protein peptides water
Molten utilization rate of fertilizer is high, and nutrition-allocated proportion is comprehensive, but its dregs of beans hydrolysis rate of protein is low, and its storage is found in actual application
Process easily goes mouldy, make base manure using when have the potential risk of increase soil-borne disease incidence.
The content of the invention
It is an object of the invention to overcome existing technological deficiency, there is provided a kind of calcic water-soluble compound fertilizer and its preparation
Technique.The method that the present invention combines step fermentation by more bacterium prepares plant active peptides, significantly improves the water of dregs of beans protein
Solution rate, content of peptides enriches in tunning, with being made after the calcium containing compound chelating such as calcium nitrate, calcium ammonium nitrate and dolomite dust
Polypeptide calcium powder chelate stability it is good, good water solubility, calcic water-soluble compound fertilizer can be mixed with a great number of elements raw material, make
Total nutrient (N+P in the calcic water-soluble compound fertilizer obtained2O5+K2O) >=40.0%, calcium oxide (CaO) >=6.0%, plant absorption
Rate is high, and nutritional ingredient not easily runs off and by soil fixing, improves utilization rate of fertilizer, reduces fertilizer cost, be advantageous to simultaneously
Increasing crop yield increases income and the raising of crops quality.
In addition, calcic water-soluble compound fertilizer provided by the invention is free of preservative, it is not susceptible to go mouldy, storage time
It is long.Meanwhile calcic water-soluble compound fertilizer provided by the invention can effectively prevent and treat the soil-borne diseases such as root rot, powdery mildew, gray mold
Evil, reduce the incidence of soil-borne disease.
Technical scheme is as follows：
A kind of preparation technology of calcic water-soluble compound fertilizer, comprises the following steps：
S1：Dregs of beans, potato, Chinese yam and tea seed cake are taken, is crushed, the water of 0.5~1.5 times of amount of its quality is added after mixing in temperature
Spend for 10~30min of wet steaming under conditions of 100~121 DEG C, sodium chloride and the aqueous solution containing 15~25% molasses added after cooling,
It is 55~70% to be adjusted after mixing to water content, obtains solid fermentation culture medium；
S2：By solid fermentation media transfer described in step S1 into fermentation tank, it is inoculated with by lactobacillus fermenti, withered grass gemma
The composite zymocyte that bacillus, Bacillus subtilis subspecies, aspergillus oryzae, aspergillus niger and Trichoderma viride are mixed, in temperature
For the 60~72h that ferments under conditions of 28~33 DEG C, fermentation process is stirred continuously；
S3：Temperature is raised to 45~60 DEG C, hydrolyzes 4~6h, hydrolytic process is stirred continuously, and hydrolysis is filtered after terminating, and takes filter
Liquid is concentrated, dry, crushing, produces plant active peptides；The filter residue obtained after filtering can also be used as organic fertilizer, make base manure administration.
S4：After calcium containing compound is melted, plant active peptides are added, chelate 45~60min, are cooled down, are crushed, polypeptide is made
Calcium powder；
S5：A great number of elements raw material is crushed, makes with local methods after granula mixes and is granulated with polypeptide calcium powder, nothing, produce calcic water-soluble
Compound fertilizer.
Further, in solid fermentation culture medium described in step S1, count in mass ratio, dregs of beans：Potato：Chinese yam：Tea seed cake：
The aqueous solution containing molasses：Sodium chloride is (80~90)：(2~8)：(10~15)：(10~20)：(15~25)：1.
Further, the inoculum concentration of composite zymocyte described in step S2 is 8~15%, i.e., per 100g solid fermentation culture mediums
It is inoculated with 8~15mL bacterium solutions.
Further, in composite zymocyte described in step S2, the inoculative proportion of each strain, lactobacillus fermenti：Withered grass gemma
Bacillus：Bacillus subtilis subspecies：Aspergillus oryzae：Aspergillus niger：Trichoderma viride is 1：(4~10)：(3~8)：(2~6)：(4
~12)：(1~3).
Lactobacillus fermenti (bacterium numbering of the present invention：BNCC337795), bacillus subtilis (bacterium numbering：
BNCC189983), Bacillus subtilis subspecies (bacterium numbering：BNCC191554), aspergillus oryzae (bacterium numbering：
AS3.863), aspergillus niger (bacterium numbering：) and Trichoderma viride (bacterium numbering BNCC336578：AS3.3711) it is conventional commercial
Commodity, it is purchased from Beijing North Na Chuanlian Bioteknologisk Institut.All zymophytes prepare through conventional actication of culture and the processing that spreads cultivation
Bacterium seed liquor or spore suspension, adjustment bacterial concentration are 1.0 × 108Individual/mL, spore concentration are 5.0 × 107Individual/mL, by inoculation
It is applied to bean pulp fermentation after ratio compounding.
Further, one kind in calcium nitrate, calcium ammonium nitrate and dolomite dust of calcium containing compound described in step S4 or
It is a variety of.
Further, the melting temperature of calcium containing compound described in step S4 is 95~105 DEG C.
Further, in step S4, count in mass ratio, calcium containing compound：Plant active peptides are (8~12)：1.
Further, a great number of elements raw material described in step S5 be selected from urea, MAP, potassium dihydrogen phosphate, potassium sulfate and
One or more in potassium nitrate.
Further, in step S5, by mass percentage, polypeptide calcium powder is 30~50%, without make with local methods granula for 2~
4%, surplus is a great number of elements raw material.
In addition, calcic water-soluble made from the preparation technology of above-mentioned calcic water-soluble compound fertilizer is also claimed in the present invention
Compound fertilizer.
In preparation technology of the present invention, using dregs of beans as main component, it is aided with potato, Chinese yam, tea seed cake, molasses and sodium chloride system
It is standby the fermented lactobacillus of solid fermentation culture medium, bacillus subtilis, Bacillus subtilis subspecies, aspergillus oryzae, black
Plant active peptides are made in aspergillus and the more bacterium joint step fermentations of Trichoderma viride, and the plant active peptides are a variety of fermentations and hydrolysis production
The mixture of thing, its main component is the soybean active polypeptide obtained after being hydrolyzed by dregs of beans protein, in addition, also containing a large amount of ammonia
Caused active material in the fermentation process such as base acid, antibacterial peptide, organic acid, plant hormone.These active materials are deposited
The chelate stability of polypeptide calcium can be not only being improved, to the growing of crops, increasing both production and income and quality improve also one
Fixed facilitation.
Dregs of beans through fully ferment, be fully hydrolyzed and filtration treatment after obtained plant active peptides and calcium containing compound chela
Close, and with obtained calcic water-soluble compound fertilizer after a great number of elements raw material mixing granulation, at ambient temperature deposit 36 months
Mildew phenomena is had no, illustrates that calcic water-soluble compound fertilizer provided by the invention is not susceptible to go mouldy, convenient storage can be for a long time
Storage.
Meanwhile the result of the test of strawberry pot experiment shows, after calcic water-soluble compound fertilizer provided by the invention,
The incidence of disease of Strawberry Root Rot, powdery mildew and gray mold significantly reduces, and calcic water-soluble compound fertilizer provided by the invention can have
The soil-borne diseases such as effect preventing and treating root rot, powdery mildew, gray mold, reduce the incidence of soil-borne disease.
Compared with prior art, the beneficial effects of the present invention are：
1) preparation technology of calcic water-soluble compound fertilizer provided by the invention, combine step fermentation by more bacterium and plant is made
Thing active peptide, significantly improve the percent hydrolysis of dregs of beans protein, plant active peptides and obtained polypeptide after calcium containing compound chelating
Calcium powder good water solubility, chelate stability is strong, and calcic water-soluble compound fertilizer can be mixed with a great number of elements raw material；
2) preparation is simple for calcic water-soluble compound fertilizer provided by the invention, and production cost is low, produces work
Skill is easily controllable, is advantageous to large-scale industrialized production；
3) calcic water-soluble compound fertilizer provided by the invention is free of preservative, is not susceptible to go mouldy, storage time length；Its
Total nutrient (N+P2O5+K2O) >=40.0%, calcium oxide (CaO) >=6.0%, plant absorption rate are high, nutritional ingredient not easily run off and
By soil fixing, utilization rate of fertilizer is improved, reduces fertilizer cost, while is advantageous to increasing crop yield and increases income and crops
The raising of quality；
4) calcic water-soluble compound fertilizer provided by the invention can effectively prevent and treat the soil such as root rot, powdery mildew, gray mold and pass
Disease, reduce the incidence of soil-borne disease.
Embodiment
The present invention is further described below by way of embodiment, but the present invention is not limited only to following examples.
In the scope of the present invention or do not departing from present disclosure, in spirit and scope, the present invention is appropriately modified, be equivalent
Change or modification, it will become apparent to those skilled in the art that they are considered as being included in the scope of the present invention
Within.
Raw materials used in the embodiment of the present invention is conventional commercial commodity.
The calcic water-soluble compound fertilizer of embodiment 1 and its preparation
The calcic water-soluble compound fertilizer of the embodiment of the present invention 1, its preparation technology comprise the following steps：
S1：Dregs of beans, potato, Chinese yam and tea seed cake are taken, is crushed, the water that 0.5 times of amount of its quality is added after mixing is in temperature
Wet steaming 20min under conditions of 121 DEG C, sodium chloride and the aqueous solution containing 15% molasses are added after cooling, is adjusted after mixing to aqueous
Measure as 55%, obtain solid fermentation culture medium；Wherein, count in mass ratio, dregs of beans：Potato：Chinese yam：Tea seed cake：Containing the water-soluble of molasses
Liquid：Sodium chloride is 80：4：15：15：25：1；
S2：By solid fermentation media transfer described in step S1 into fermentation tank, it is inoculated with by lactobacillus fermenti, withered grass gemma
The composite zymocyte that bacillus, Bacillus subtilis subspecies, aspergillus oryzae, aspergillus niger and Trichoderma viride are mixed, inoculum concentration
For 8%, fermented 72h under conditions of being 28 DEG C in temperature, and fermentation process is stirred continuously；Wherein, in the composite zymocyte, each bacterium
The inoculative proportion of kind, lactobacillus fermenti：Bacillus subtilis：Bacillus subtilis subspecies：Aspergillus oryzae：Aspergillus niger：Green
Trichoderma is 1：10：3：2：4：3, lactobacillus fermenti, bacillus subtilis, the bacterium solution of Bacillus subtilis subspecies seed liquor are dense
Spend for 1.0 × 108Individual/mL, the spore concentration of aspergillus oryzae, aspergillus niger and Trichoderma viride spore suspension is 5.0 × 107Individual/mL；
S3：Temperature is raised to 45 DEG C, hydrolyzes 6h, hydrolytic process is stirred continuously, and hydrolysis is filtered after terminating, and takes filtrate through dense
Contracting, dry, crush, producing plant active peptides；
S4：By calcium nitrate and dolomite dust in mass ratio 1:After 1 mixing after 105 DEG C of meltings, plant active peptides, chela are added
45min is closed, is cooled down, is crushed, polypeptide calcium powder is made；Wherein, count in mass ratio, calcium containing compound：Plant active peptides are 9：1；
S5：41% MAP and 20% potassium sulfate are crushed, with 36% polypeptide calcium powder, 3% without making with local methods after granula mixes
It is granulated in rotary drum granulator, produces the calcic water-soluble compound fertilizer of the embodiment of the present invention 1.
The calcic water-soluble compound fertilizer of embodiment 2 and its preparation
The calcic water-soluble compound fertilizer of the embodiment of the present invention 2, its preparation technology comprise the following steps：
S1：Dregs of beans, potato, Chinese yam and tea seed cake are taken, is crushed, the water that 1.5 times of amounts of its quality are added after mixing is in temperature
Wet steaming 30min under conditions of 121 DEG C, sodium chloride and the aqueous solution containing 25% molasses are added after cooling, is adjusted after mixing to aqueous
Measure as 70%, obtain solid fermentation culture medium；Wherein, count in mass ratio, dregs of beans：Potato：Chinese yam：Tea seed cake：Containing the water-soluble of molasses
Liquid：Sodium chloride is 90：8：10：20：15：1；
S2：By solid fermentation media transfer described in step S1 into fermentation tank, it is inoculated with by lactobacillus fermenti, withered grass gemma
The composite zymocyte that bacillus, Bacillus subtilis subspecies, aspergillus oryzae, aspergillus niger and Trichoderma viride are mixed, inoculum concentration
For 15%, fermented 60h under conditions of being 33 DEG C in temperature, and fermentation process is stirred continuously；Wherein, in the composite zymocyte, respectively
The inoculative proportion of strain, lactobacillus fermenti：Bacillus subtilis：Bacillus subtilis subspecies：Aspergillus oryzae：Aspergillus niger：It is green
Color trichoderma is 1：4：8：6：12：1, lactobacillus fermenti, bacillus subtilis, the bacterium solution of Bacillus subtilis subspecies seed liquor
Concentration is 1.0 × 108Individual/mL, the spore concentration of aspergillus oryzae, aspergillus niger and Trichoderma viride spore suspension is 5.0 × 107Individual/mL；
S3：Temperature is raised to 60 DEG C, hydrolyzes 4h, hydrolytic process is stirred continuously, and hydrolysis is filtered after terminating, and takes filtrate through dense
Contracting, dry, crush, producing plant active peptides；
S4：By calcium nitrate and dolomite dust in mass ratio 1:After 1 mixing after 105 DEG C of meltings, plant active peptides, chela are added
45min is closed, is cooled down, is crushed, polypeptide calcium powder is made；Wherein, count in mass ratio, calcium containing compound：Plant active peptides are 9：1；
S5：16% potassium dihydrogen phosphate and 42.5% potassium nitrate are crushed, granula is made with local methods with 39.5% polypeptide calcium powder, 2% nothing
Granulation, produces the calcic water-soluble compound fertilizer of the embodiment of the present invention 2 after being blended in granulating disc.
The calcic water-soluble compound fertilizer of embodiment 3 and its preparation
The calcic water-soluble compound fertilizer of the embodiment of the present invention 3, its preparation technology comprise the following steps：
S1：Dregs of beans, potato, Chinese yam and tea seed cake are taken, is crushed, the water that 0.8 times of amount of its quality is added after mixing is in temperature
Wet steaming 20min under conditions of 121 DEG C, sodium chloride and the aqueous solution containing 20% molasses are added after cooling, is adjusted after mixing to aqueous
Measure as 65%, obtain solid fermentation culture medium；Wherein, count in mass ratio, dregs of beans：Potato：Chinese yam：Tea seed cake：Containing the water-soluble of molasses
Liquid：Sodium chloride is 88：5：15：15：20：1；
S2：By solid fermentation media transfer described in step S1 into fermentation tank, it is inoculated with by lactobacillus fermenti, withered grass gemma
The composite zymocyte that bacillus, Bacillus subtilis subspecies, aspergillus oryzae, aspergillus niger and Trichoderma viride are mixed, inoculum concentration
For 10%, fermented 72h under conditions of being 30 DEG C in temperature, and fermentation process is stirred continuously；Wherein, in the composite zymocyte, respectively
The inoculative proportion of strain, lactobacillus fermenti：Bacillus subtilis：Bacillus subtilis subspecies：Aspergillus oryzae：Aspergillus niger：It is green
Color trichoderma is 1：6：6：5：10：2, lactobacillus fermenti, bacillus subtilis, the bacterium solution of Bacillus subtilis subspecies seed liquor
Concentration is 1.0 × 108Individual/mL, the spore concentration of aspergillus oryzae, aspergillus niger and Trichoderma viride spore suspension is 5.0 × 107Individual/mL；
S3：Temperature is raised to 55 DEG C, hydrolyzes 5h, hydrolytic process is stirred continuously, and hydrolysis is filtered after terminating, and takes filtrate through dense
Contracting, dry, crush, producing plant active peptides；
S4：By calcium ammonium nitrate after 100 DEG C of meltings, plant active peptides are added, chelate 50min, are cooled down, are crushed, are made more
Peptide calcium powder；Wherein, count in mass ratio, calcium containing compound：Plant active peptides are 11.5：1；
S5：By 7.5% urea, 30% potassium dihydrogen phosphate and 16.5% MAP crush, with 44% polypeptide calcium powder, 2%
Without being granulated after making granula mixing with local methods in granulating disc, the calcic water-soluble compound fertilizer of the embodiment of the present invention 3 is produced.
The calcic water-soluble compound fertilizer of embodiment 4 and its preparation
The calcic water-soluble compound fertilizer of the embodiment of the present invention 4, its preparation technology comprise the following steps：
S1：Dregs of beans, potato, Chinese yam and tea seed cake are taken, is crushed, the water that 1.0 times of amounts of its quality are added after mixing is in temperature
Wet steaming 15min under conditions of 121 DEG C, sodium chloride and the aqueous solution containing 20% molasses are added after cooling, is adjusted after mixing to aqueous
Measure as 65%, obtain solid fermentation culture medium；Wherein, count in mass ratio, dregs of beans：Potato：Chinese yam：Tea seed cake：Containing the water-soluble of molasses
Liquid：Sodium chloride is 90：6：12：15：20：1；
S2：By solid fermentation media transfer described in step S1 into fermentation tank, it is inoculated with by lactobacillus fermenti, withered grass gemma
The composite zymocyte that bacillus, Bacillus subtilis subspecies, aspergillus oryzae, aspergillus niger and Trichoderma viride are mixed, inoculum concentration
For 10%, fermented 72h under conditions of being 32 DEG C in temperature, and fermentation process is stirred continuously；Wherein, in the composite zymocyte, respectively
The inoculative proportion of strain, lactobacillus fermenti：Bacillus subtilis：Bacillus subtilis subspecies：Aspergillus oryzae：Aspergillus niger：It is green
Color trichoderma is 1：5：5：4：8：3, lactobacillus fermenti, bacillus subtilis, the bacterium solution of Bacillus subtilis subspecies seed liquor
Concentration is 1.0 × 108Individual/mL, the spore concentration of aspergillus oryzae, aspergillus niger and Trichoderma viride spore suspension is 5.0 × 107Individual/mL；
S3：Temperature is raised to 50 DEG C, hydrolyzes 5h, hydrolytic process is stirred continuously, and hydrolysis is filtered after terminating, and takes filtrate through dense
Contracting, dry, crush, producing plant active peptides；
S4：By calcium ammonium nitrate after 100 DEG C of meltings, plant active peptides are added, chelate 50min, are cooled down, are crushed, are made more
Peptide calcium powder；Wherein, count in mass ratio, calcium containing compound：Plant active peptides are 11.5：1；
S5：40% MAP and 18.5% potassium sulfate are crushed, granula is made with local methods with 39% polypeptide calcium powder, 2.5% nothing and mixes
It is granulated after conjunction in granulating disc, produces the calcic water-soluble compound fertilizer of the embodiment of the present invention 4.
Comparative example 1
Compared with Example 3, this comparative example calcic water-soluble compound fertilizer differs only in：In step of preparation process S1
Solid fermentation culture medium is free of Chinese yam.
Comparative example 2
Compared with Example 3, this comparative example calcic water-soluble compound fertilizer differs only in：In step of preparation process S1
Solid fermentation culture medium is free of tea seed cake.
Comparative example 3
Compared with Example 3, this comparative example calcic water-soluble compound fertilizer differs only in：In step of preparation process S2
Composite zymocyte is free of Bacillus subtilis subspecies.
Comparative example 4
Compared with Example 3, this comparative example calcic water-soluble compound fertilizer differs only in：In step of preparation process S2
Composite zymocyte is free of Trichoderma viride.
Comparative example 5
Compared with Example 3, this comparative example calcic water-soluble compound fertilizer differs only in：Step of preparation process S2
In, fermentation time 77h, filter after fermentation ends, take concentrating filter liquor, drying, crushing, produce plant active peptides, without step
Rapid S3.
Test example one, wheat germination experiment
1st, test specimen：Calcic water-soluble compound fertilizer made from present invention implementation 1-4, comparative example 5, is dissolved with distilled water
Solid-to-liquid ratio 1 is pressed afterwards：500 times of dilutions are standby.
2nd, test method：Uniform in size, full seed wheat seed is chosen, divides 1 group of embodiment, 2 groups of embodiment, implement
6 groups of 3 groups of example, 4 groups of embodiment, 5 groups of comparative example and blank control group, 100 wheat seeds are one group, and each group wheat seed is used
Soaked seed 4 hours after 3% disinfectant with hydrogen peroxide, wherein, 5 groups of embodiment 1-4 groups, comparative example wheat seeds are used respectively contains embodiment
The solution for the calcic water-soluble compound fertilizer that 1-4, comparative example 5 are prepared is soaked seed, and blank control group wheat seed steams
Distilled water is soaked seed.After seed soaking terminates, wheat seed is put into the culture dish for be covered with filter paper and carries out germination test, during experiment
Filter paper keeps soaking with distilled water.Each group germinated wheat after the germination percentage and 72h and 96h of each group wheat after record 24h and 48h
Radical, stem length and the root long of seed, average.
3rd, result of the test：See Tables 1 and 2.
The wheat germination rate of table 1
Radical, stem length and the root long of the germinated wheat seed of table 2
As shown in Table 1, the germination percentage after embodiment 1-4 groups wheat seed 24h be significantly higher than blank control group and
5 groups of comparative example, illustrate that calcic water-soluble compound fertilizer of the present invention can effectively facilitate quick germination of wheat seeds, improve wheat seed
Germination percentage.
As shown in Table 2, the radical of embodiment 1-4 groups germinated wheat seed and 5 groups of blank control group or comparative example
Compare, no marked difference, but the stem length of embodiment 1-4 group germinated wheat seeds and root long are considerably longer than blank control group and right
5 groups of ratio, illustrate that calcic water-soluble compound fertilizer of the present invention can effectively facilitate the growth of germinated wheat kind blastostyle and root.Wherein,
Calcic water-soluble compound fertilizer made from embodiment 3 promotes the effect of crop growth best.
Test example two, strawberry pot experiment
1st, test specimen：Calcic water-soluble compound fertilizer made from embodiment 3, comparative example 1-4.
2nd, trial crops：Strawberry cultivars are " Feng Xiang ", and Strawberry Seedlings are the heart seedling of 5 leaf 1, and balled transplanting is into basin alms bowl.
3rd, test method：Potting soil is continuous cropping soil, is derived from strawberry continuous cropping plot, native 5kg is filled per basin, per potted plant kind one
Strawberry Seedlings.Pot experiment sets 6 processing altogether, and each processing sets 50 repetitions, and processing 1-5 applies embodiment 3, comparative example respectively
Calcic water-soluble compound fertilizer made from 1-4, processing 6 is control group, using by urea, potassium dihydrogen phosphate, MAP and nitre
Water-soluble compound fertilizer made from sour ammonium calcium, by adjusting sowing amount, make (the N+P of every processing application2O5+K2O) total nutrient content
It is identical.
After strawberry transplanting, continuous plantation 9 months, root rot, powdery mildew and gray mold neopathy are recorded during plantation in time
And incidence, the incidence of the strawberry root root rot of all processing is observed after off-test.During statistical test
The incidence of disease of Strawberry Root Rot, powdery mildew and gray mold：The incidence of disease=processing disease plant number/processing total strain number × 100%.
4th, result of the test：It is shown in Table 3.
The strawberry soil-borne disease incidence of table 3
| Processing | Root rot (%) | Powdery mildew (%) | Gray mold (%) |
| Embodiment 3 | 8 | 2 | 0 |
| Comparative example 1 | 20 | 6 | 8 |
| Comparative example 2 | 22 | 6 | 10 |
| Comparative example 3 | 24 | 8 | 10 |
| Comparative example 4 | 20 | 6 | 6 |
| Control group | 26 | 8 | 12 |
As shown in Table 3, compared with control group, calcic water-soluble compound fertilizer is to root-rot made from comparative example 1-4
The prevention effect unobvious of the soil-borne diseases such as disease, powdery mildew, gray mold.And it is compound to apply calcic water-soluble made from embodiment 3
After fertilizer, the incidence of disease of Strawberry Root Rot, powdery mildew and gray mold significantly reduces, and illustrates that calcic water-soluble provided by the invention is answered
Hefei material can effectively prevent and treat the soil-borne diseases such as root rot, powdery mildew, gray mold, reduce the incidence of soil-borne disease.
Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to is assert
The specific implementation of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of not departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's
Protection domain.
Claims (8)
1. a kind of preparation technology of calcic water-soluble compound fertilizer, it is characterised in that comprise the following steps：
S1：Dregs of beans, potato, Chinese yam and tea seed cake are taken, is crushed, the water that 0.5~1.5 times of amount of its quality is added after mixing is in temperature
10~30min of wet steaming under conditions of 100~121 DEG C, sodium chloride and the aqueous solution containing 15~25% molasses are added after cooling, mixed
After adjust to water content be 55~70%, obtain solid fermentation culture medium；
S2：By solid fermentation media transfer described in step S1 into fermentation tank, it is inoculated with by lactobacillus fermenti, bacillus subtilis
The composite zymocyte that bacterium, Bacillus subtilis subspecies, aspergillus oryzae, aspergillus niger and Trichoderma viride are mixed, it is in temperature
Fermented 60~72h under conditions of 28~33 DEG C, and fermentation process is stirred continuously；
S3：Temperature is raised to 45~60 DEG C, hydrolyzes 4~6h, hydrolytic process is stirred continuously, and hydrolysis is filtered after terminating, and takes filtrate to pass through
Concentration, dry, crush, producing plant active peptides；
S4：After calcium containing compound is melted, plant active peptides are added, chelate 45~60min, are cooled down, are crushed, polypeptide calcium is made
Powder；
S5：A great number of elements raw material is crushed, makes with local methods after granula mixes and is granulated with polypeptide calcium powder, nothing, it is compound to produce calcic water-soluble
Fertilizer；
In solid fermentation culture medium described in step S1, count in mass ratio, dregs of beans：Potato：Chinese yam：Tea seed cake：Containing the water-soluble of molasses
Liquid：Sodium chloride is (80~90)：(2~8)：(10~15)：(10~20)：(15~25)：1；
In composite zymocyte described in step S2, the inoculative proportion of each strain, lactobacillus fermenti：Bacillus subtilis：Withered grass gemma
Bacillus withered grass subspecies：Aspergillus oryzae：Aspergillus niger：Trichoderma viride is 1：(4~10)：(3~8)：(2~6)：(4~12)：(1~3).
2. the preparation technology of calcic water-soluble compound fertilizer as claimed in claim 1, it is characterised in that compound described in step S2
Zymogenic inoculum concentration is 8~15%.
3. the preparation technology of calcic water-soluble compound fertilizer as claimed in claim 1, it is characterised in that calcic described in step S4
One or more of the compound in calcium nitrate, calcium ammonium nitrate and dolomite dust.
4. the preparation technology of calcic water-soluble compound fertilizer as claimed in claim 1, it is characterised in that calcic described in step S4
The melting temperature of compound is 95~105 DEG C.
5. the preparation technology of calcic water-soluble compound fertilizer as claimed in claim 1, it is characterised in that in step S4, by matter
Amount is than meter, calcium containing compound：Plant active peptides are (8~12)：1.
6. the preparation technology of calcic water-soluble compound fertilizer as claimed in claim 1, it is characterised in that a large amount of described in step S5
One or more of the element raw material in urea, MAP, potassium dihydrogen phosphate, potassium sulfate and potassium nitrate.
7. the preparation technology of calcic water-soluble compound fertilizer as claimed in claim 1, it is characterised in that in step S5, by matter
Percentage meter is measured, polypeptide calcium powder is 30~50%, is 2~4% without granula is made with local methods, surplus is a great number of elements raw material.
8. calcic water-soluble made from the preparation technology of the calcic water-soluble compound fertilizer as described in claim 1~7 is any is answered
Expect in Hefei.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61209981A (en) * | 1985-03-12 | 1986-09-18 | 片倉チツカリン株式会社 | Manufacture of fertilizer |
| CN101139227A (en) * | 2006-09-06 | 2008-03-12 | 张培樱 | Organic liquid fertilizer |
| CN102550874A (en) * | 2012-01-18 | 2012-07-11 | 安徽燕之坊食品有限公司 | Method for producing active polypeptide rich product by solid state fermentation and application |
| CN102660622A (en) * | 2012-04-28 | 2012-09-12 | 浙江大飞龙动物保健品有限公司 | Method for preparing bioactive soybean peptide by mixed culture solid-state fermentation |
| CN105272478A (en) * | 2015-11-17 | 2016-01-27 | 烟台泓源生物肥料有限公司 | Water-soluble protein peptide fertilizer |
-
2017
- 2017-04-18 CN CN201710254558.6A patent/CN106977292B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61209981A (en) * | 1985-03-12 | 1986-09-18 | 片倉チツカリン株式会社 | Manufacture of fertilizer |
| CN101139227A (en) * | 2006-09-06 | 2008-03-12 | 张培樱 | Organic liquid fertilizer |
| CN102550874A (en) * | 2012-01-18 | 2012-07-11 | 安徽燕之坊食品有限公司 | Method for producing active polypeptide rich product by solid state fermentation and application |
| CN102660622A (en) * | 2012-04-28 | 2012-09-12 | 浙江大飞龙动物保健品有限公司 | Method for preparing bioactive soybean peptide by mixed culture solid-state fermentation |
| CN105272478A (en) * | 2015-11-17 | 2016-01-27 | 烟台泓源生物肥料有限公司 | Water-soluble protein peptide fertilizer |
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