CN106975012A - A kind of traditional Chinese medicinal composition with effect of reducing blood sugar and preparation method thereof - Google Patents
A kind of traditional Chinese medicinal composition with effect of reducing blood sugar and preparation method thereof Download PDFInfo
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- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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Abstract
Constituted the invention discloses a kind of traditional Chinese medicinal composition with effect of reducing blood sugar, including following parts by weight traditional Chinese medicine ingredients：4 10 parts of sealwort：03 parts of radix polygonati officinalis：03 parts of mulberry leaf.The composition that the Chinese medicine composition of the present invention is selected is relatively simple, there is good coordinated relation between composition, it is respectively belonging to lung channel, kidney channel, meet the traditional Chinese medical science for " diabetes " treatment concept, being capable of nourishing kidney-YIN, promote the production of body fluid fluid infusion, establishing-Yang qi-restoratives, the effect of patient's kidney yin is adjusted with specific aim, strengthen the body resistance to consolidate the constitution, QI invigorating is quenched the thirst, with preferable effect of lowering blood sugar, suitable product form can be arbitrarily selected in the case where not influenceing composition efficacy exertion, food can be made, beverage, health food, tea bag, the tablet of original new drug, capsule, formulations such as oral liquid etc., using convenient, simply, it is easy.
Description
Technical field
The present invention relates to a kind of traditional Chinese medicinal composition with effect of reducing blood sugar, more particularly to a kind of drop containing sealwort, radix polygonati officinalis and mulberry leaf
Hypoglycemic medicine composition is pressed, belongs to technical field of Chinese medicine.
Background technology
Diabetes are the common chronic diseases for threatening human health, and diabetic is prevalent in vast social crowd.Closely
Nian Lai, physical resources increasingly enrich, and manual labor amount is fewer and fewer, and fat, diabetic's number increases year by year.
It is existing to have there are many chemicalses to be widely used for the treatment of diabetes.But due to these chemicalses
The material of non-body active absorption, can promptly be excluded after administration by body, therefore be merely capable of playing limited treatment
Value.In order to obtain desired therapeutic effect, dosage must be just increased, heavy dose of quality medicine can cause body again
Negative-feedback, reduces the effect of medicine.
Chinese medicine crude drug is that naturally occurring plant, animal drug, particularly vegetable drug and general food have very
Good similitude, is easily absorbed for human body, with good compatibility.Study of Traditional Chinese Medicine crude drug is in reduction blood glucose
Using with exploring potential ability well, being hopeful to provide a kind of natural drug composition with good friendly, be particularly
Natural plant crude drugs composition.
For example Chinese patent CN101455789A discloses the Chinese medicine composition to hypoglycemic diuresis, its disclosed composition select compared with
To be numerous and diverse, and composition mutual cooperation relation therein is not clear, and in-depth study is not inquired into, and one side mechanism is failed to understand, separately
On the one hand the method for research lacks normative, without actual directive significance.
Natural plant crude drugs want to have given play to real pharmacodynamic feature in application in TCM field, in addition it is also necessary to in-depth study
Analysis, on the one hand will study the cure mechanism of medicine, on the other hand effective component and non-effective component are distinguished by, it is determined that
Effective composition, discharges the interference of bad or invalid composition.While the drug effect of pharmaceutical composition is lifted, reduction medicine
Adverse reaction influence etc..
The content of the invention
It is an object of the invention to overcome the Chinese medicine hypoglycemic research in the presence of prior art less, effect that medicine is played
There is provided a kind of traditional Chinese medicinal composition with effect of reducing blood sugar for indefinite problem.The Chinese medicine composition of the present invention passes through scientific validation research, point
Analyse the mutually direct influence of drug ingedient, it is proposed that the Traditional Chinese medicine composition formula of science, there is reliable, peace for hypoglycemic
Full effect.
In order to realize foregoing invention purpose, the invention provides following technical scheme：
A kind of traditional Chinese medicinal composition with effect of reducing blood sugar, including following parts by weight traditional Chinese medicine ingredients composition：4-10 parts of sealwort：Radix polygonati officinalis 0-3
Part：0-3 parts of mulberry leaf.
The composition that the Chinese medicine composition of the present invention is selected is relatively simple, but the cooperation collaboration rich in traditional Chinese medicine ingredients each other
Relation.The three taste traditional Chinese medicine ingredients wherein selected all are the partially sweet flat food and medicine consangunity compositions of nature and flavor, are respectively belonging to lung, kidney channel arteries and veins,
Meet the traditional Chinese medical science for " diabetes " treatment concept.The traditional Chinese medical science thinks that diabetes is that patient diet is irregular, stagnated fire impairment of yin, and the five internal organs are empty
Weak, damage of kidney-essence crosses the product for taking temperate-dryness, and consumption liquid is hindered caused by Tianjin.Sealwort, radix polygonati officinalis and mulberry leaf can nourishing kidney-YIN, fluid infusion of promoting the production of body fluid, strengthen
Positive qi-restoratives, the effect of patient's kidney yin is adjusted with specific aim.After combining, several compositions each other there is collaboration to increase
Effect is acted on, and be can be good at promoting the body transporting of patient's body, is strengthened the body resistance to consolidate the constitution, QI invigorating is quenched the thirst, reach hypoglycemic effect.
Further, the weight ratio of each composition is in the Chinese medicine composition：4-8 parts of sealwort：1-2 parts of radix polygonati officinalis：Mulberry leaf
1-2 parts.The Chinese medicine composition of the present invention uses its boosting qi and nourishing yin using sealwort as main component, and moistening lung, effect of kidney-nourishing coordinates beautiful
Bamboo and mulberry leaf, which are moisturized, to promote the production of body fluid, and effect that clearing lung-heat is reduced internal heat reaches effect of concertedness conditioning blood sugar.
Further, the weight ratio of each composition is in the Chinese medicine composition：2-4 parts of sealwort：1 part of radix polygonati officinalis：Mulberry leaf 1
Part.
Compared with prior art, beneficial effects of the present invention：
It is using the authenticated Chinese medicine group of the method test of science 1. the Chinese medicine composition of the present invention is relative to existing research
Compound is formulated, all targeted per traditional Chinese medicine ingredients simply although its composition is simple, can well be cooperateed with and given play to together
Effect of blood glucose is reduced, hypoglycemic effect is more notable.
2. the raw material of the Chinese medicine composition of the present invention is selected from the material of food and medicine consangunity, safe and reliable, it is adaptable to sub-healty adults
Group's edible health, preventative hypoglycemic, quality of making the life better lifts quality of the life, can reach what is preventiveed treatment of disease with long-term taking
Purpose, and it is economical and practical, health and the health product of sub-health population can be used as.
3. in the Traditional Chinese medicine composition formula of the reduction blood glucose of the present invention, the mixing ratio of sealwort, radix polygonati officinalis and mulberry leaf is suitable, energy
The effect of lowering blood sugar of enough maximized performance drug regimens together, can reach optimal treatment work(with minimum drug dose
Effect, with it is evident in efficacy prominent the characteristics of, in terms of dosage have advantage, liver kidney Small side effects during medication.
4. in the Traditional Chinese medicine composition formula of the reduction blood glucose of the present invention, sealwort, radix polygonati officinalis and the taste medicine of mulberry leaf three composition are and similar
Hypoglycemic composition compare, flavour of a drug are few, and zoopery shows that hypoglycemic effect is good, stable and controllable for quality, reproducible.
Brief description of the drawings：
Fig. 1 is each experimental group rat blood serum FFA concentrations.
Fig. 2 is each experimental group rat blood serum insulin concentration.
Fig. 3 is IL-6 concentration in each experimental group rat blood serum.
Fig. 4 is TNF-α concentration in each experimental group rat blood serum.
Embodiment
The method of Chinese medicine composition of the present invention is processed it is a further object of the present invention to provide a kind of, or prepares Chinese medicine group
The method of compound, comprises the following steps：
(1) sealwort, radix polygonati officinalis and mulberry leaf, chopping or section are taken, 1-20 times of water is added, immersion is decocted 1-5 times, each 0.1-3
Hour；
(2) decoction liquor, filtering, merging filtrate are collected；Centrifugation, merge supernatant, be concentrated into 0.2g/mL containing crude drug amount with
On.
The material composition that the present invention is selected：Sealwort, radix polygonati officinalis and mulberry leaf, are all the compositions of property sweet taste, drug effect therein into
Divide based on water miscible composition, also indicate that extraction water soluble ingredient therein for hypoglycemic work(with reference to the research of the present invention
Effect is realized with more important meaning.Existing Chinese medicine ancient books and records for sealwort, radix polygonati officinalis and mulberry leaf commentary approximately as：
Sealwort, nature and flavor：It is sweet, put down.Channel tropism：Returns spleen, lung, kidney channel.Major function：Boosting qi and nourishing yin, invigorating the spleen, moistening lung, kidney-nourishing.With
In weakness of the spleen and the stomach, fatigue and asthenia, dry deficiency of food, deficiency syndrome of the lung cough caused by dryness, asthenia of essence and blood, Heat Diabetes.
Radix polygonati officinalis, nature and flavor：It is sweet, it is slightly cold.Channel tropism：Attach to the lung and stomach meridians.Major function：Nourishing yin to moisten dryness, promotes the production of body fluid to quench thirst.For lung stomach
Impairment of yin, cough caused by dryness-heat, dry throat and mouth, Heat Diabetes.
Mulberry leaf, nature and flavor：Sweet, hardship, trembles with fear.Channel tropism：Return lung, Liver Channel.Major function：Dispelling wind and heat from the body, clearing away the lungheat and moisturizing is cleared liver bright
Mesh.For anemopyretic cold, lung-heat type cough, dizziness headache, hot eyes is dim-sighted.
Three kinds of traditional Chinese medicine ingredients enter return lung, Liver Channel and kidney channel, and effect mainly improves therapeutic effect using the class that promotes the production of body fluid to quench thirst as mechanism.
Can be had using water extraction and targetedly extract water-soluble natural drug ingedient therein, with good selectivity, passed through
The effective component synergy that verification experimental verification water extraction is extracted is good, and hypoglycemic effect is stronger, has no toxic side effect.
It is further preferred that step 1 in method, chopping to maximum gauge is less than 2-5mm, or it is sliced into thickness 1-4mm
Piece.Bulk drug is soaked decoction after chopping or slicing treatment, conveniently and extracted.During extraction is decocted, fragment
Crude drug have bigger with water contact surface area, improve the efficiency of leaching, the carry out medicinal material that can also be more prone to is stirred
Mix.
It is further preferred that step 1 in method, adds 5-12 times of water immersion.Immersion water is in follow-up extraction process
Used directly as extract solution, so 5-12 times of soak is more suitable, for decocting in extraction in dissolving medicinal material
Effective component is also more strong.
It is further preferred that step 1 in method, more preferably soak time 5-60min, preferably 10-50min, 20-35min.
Three kinds of Chinese medicine crude drug compositions that the present invention is used are the crude drug of dry lump shape, and after immersion for a period of time, medicinal material fills
Divide water suction infiltration, then can lift the efficiency of decoction.If medicinal material is without immersion treatment, easily there is the drying of medicinal material center
Do not infiltrate and can not obtain fully decocting the defect extracted.
It is further preferred that step 1 in method, decocts number of times 2-4 times.Find to decoct by the numerous studies of inventor
Boil medicinal material after suitable number of times fully to be extracted, content of drug effect components is less in the dregs of a decoction, continue to decoct economic value more than decoction
The cost of consumption, it is advantageous to decoct number of times to be advisable for 2-4 times.
It is further preferred that step 1 in method, each decocting time 20-60min is advisable, preferably 20-45min.Decocted
The speed of release is dissolved in journey according to the native chemical composition of medicinal material, weighs the advantages and disadvantages, is advisable within preferably decocting time 20-60 minutes,
Preferably 20-45 minutes, native chemical composition obtained more composition release, and the energy-output ratio of decoction is also controlled in relatively low water
It is flat.
It is further preferred that step 2 in method, filtered through gauze during filtering.Filtered through gauze is for containing a large amount of dregs of a decoction
For decoction liquor, with filter effect preferably, the advantage of speed.Avoid filtration time oversize, decoction exposes in atmosphere
Overlong time plays ingredients from lossing or the increase of rotten risk, and many factors can be more weighed with filtered through gauze.And coordinate
Follow-up centrifugal treating, the effect for not influenceing processing of crude drugs to handle.
It is further preferred that step 2 in method, centrifugal rotational speed is more than 1500r/min, preferably greater than 3000r/min, have
The centrifugation of effect is gone out the dregs of a decoction.Centrifugal speed should be not less than 1500 revs/min, it is ensured that small particle in the dregs of a decoction after filtering
Impurity can be by the bottom of sufficient centrifugal sedimentation to decoction.
It is further preferred that being concentrated in step 2 in method, concentration process using rotary evaporation.Preferably, it is concentrated into containing life
More than dose 0.4g/mL, is finally that 0.5g/mL containing crude drug amount or so is advisable, such as 0.4-0.6g/mL containing crude drug amount.After concentration
Decoction so that containing crude drug amount, as more than 0.4g/mL, below 0.6g/mL is optimal, appropriate medicinal ingredient is both contained in decoction, and not
Deposit the problem of concentration is higher to be unfavorable for effective component dissolving release utilization.
With reference to test example and embodiment, the present invention is described in further detail.But this should not be understood
Following embodiment is only limitted to for the scope of above-mentioned theme of the invention, it is all that this is belonged to based on the technology that present invention is realized
The scope of invention.
【Embodiment 1】
8 grams of sealwort, 2 grams of radix polygonati officinalis, 2 grams of mulberry leaf are taken, mixes, obtains traditional Chinese medicinal composition with effect of reducing blood sugar.
【Embodiment 2】
7 grams of sealwort, 1 gram of radix polygonati officinalis, 2 grams of mulberry leaf are taken, is shredded, is clayed into power, obtain traditional Chinese medicinal composition with effect of reducing blood sugar, be distributed into
Tea bag, 3-5 grams/bag.
【Embodiment 3】
6 grams of sealwort, 2 grams of radix polygonati officinalis, 3 grams of mulberry leaf are taken, are shredded, cryogrinding powder obtains traditional Chinese medicinal composition with effect of reducing blood sugar, plus
Enter pharmacy acceptable auxiliary material, it is tabletted.
【Embodiment 4】
8 grams of sealwort, 3 grams of radix polygonati officinalis, 2 grams of mulberry leaf are taken, shreds, mixes, cryogenic pulverization to particle diameter is less than 1mm, is made
Capsule preparations.
【Embodiment 5】
Take 20 grams of sealwort, 5 grams of radix polygonati officinalis, 4 grams of mulberry leaf, shred, add 325mL clear water, be cooked by slow fire 2 hours, obtain sealwort,
Radix polygonati officinalis and the Chinese medicine composition decocting liquid of mulberry leaf.This decocting liquid is concentrated into 0.4g/mL containing raw medicinal herbs, polyethylene oral liquid bottle is sub-packed in
In, obtain oral liquid product.
【Embodiment 6】
40 grams of sealwort, 10 grams of radix polygonati officinalis, 10 grams of mulberry leaf, plus 550mL water are taken, 30min is soaked, boils three times, each 1h, collects and decocts
After boil liquid, filtering, merging filtrate, high speed centrifugation 10min, merge supernatant, use Rotary Evaporators concentrated extracting solution, be concentrated into and contain
Crude drug amount 0.4g/mL.This concentrate is evaporated to dryness, pharmacy acceptable auxiliary material is added, is distributed into capsule preparations.
【Embodiment 7】
50 grams of sealwort, 12 grams of radix polygonati officinalis, 10 grams of mulberry leaf, plus 600mL water are taken, 20min is soaked, boils four times, each 1.5h, is collected
After decoction liquor, filtered through gauze, merging filtrate, centrifugation 20min, merge supernatant, use Rotary Evaporators concentrated extracting solution, be concentrated into
0.5g/mL containing crude drug amount.
【Embodiment 8】
4 grams of sealwort, 1 gram of radix polygonati officinalis, 1 gram of mulberry leaf are taken, is mixed, is obtained traditional Chinese medicinal composition with effect of reducing blood sugar, be packaged into pouch, be used as food
Product added material.
A variety of different product forms can be made in the sealwort of the present invention, the composition of radix polygonati officinalis and mulberry leaf, be not limited to above-mentioned
The product type referred to, suitable product form can be arbitrarily selected in the case where not influenceing composition efficacy exertion, can be with
Make food, beverage, health food, tea bag, the formulation such as tablet, capsule, the oral liquid of original new drug etc..
【Pharmacology checking test】
First, material
1.1 animals and reagent
Male SD rat 200 (reaches large) purchased from Chengdu, 200 ± 20g of body weight, free diet, natural lighting.Chain urea helps bacterium
Plain (STZ)；Melbine；Polygonatum Solomon's seal mulberry leaf different ratio decocting liquid；High-sugar-fat-diet (be purchased from Chengdu up to large)；Serum is swum
From aliphatic acid kit, IL-6 kits, TNF-α kit, Insulin Kit (is purchased from the limited public affairs of Chengdu Constant science and technology
Department)
1.2 the preparation to drug solns
1.2.1 melbine
A piece of melbine (0.85g), plus 50ml distilled water, are dissolved, are produced.
1.2.2 the decocting liquid of polygonatum Solomon's seal mulberry leaf different ratio
Sealwort is liliaceous plant David's-harp Polygonatum cyrtonema Hua dry rhizome；
Radix polygonati officinalis is liliaceous plant radix polygonati officinalis Polygonatum odoratum (Mill.) Druce dry rhizome；
Mulberry leaf are moraceae plants mulberry Morus alba L. dried leaf.
It is divided into according to sealwort, radix polygonati officinalis, mulberry leaf different ratio：
A group sealworts：Radix polygonati officinalis：Mulberry leaf (2：1：1)
B group sealworts：Radix polygonati officinalis：Mulberry leaf (3：1：1,
C group sealworts：Radix polygonati officinalis：Mulberry leaf (4：1：1)
D group sealworts：Mulberry leaf：Radix polygonati officinalis (2：1：0)
E group sealworts：Mulberry leaf：Radix polygonati officinalis (2：0：1)
F group sealworts：Mulberry leaf：Radix polygonati officinalis (3：1：0)
G group sealworts：Mulberry leaf：Radix polygonati officinalis (3：0：1)
H group sealworts：Mulberry leaf：Radix polygonati officinalis (4：1：0)
I group sealworts：Mulberry leaf：Radix polygonati officinalis (4：0：1)
J group sealworts：Mulberry leaf：Radix polygonati officinalis (0：1：1)
K group sealworts：Mulberry leaf：Radix polygonati officinalis (1：0：0)
L group sealworts：Mulberry leaf：Radix polygonati officinalis (0：1：0)
M group sealworts：Mulberry leaf：Radix polygonati officinalis (0：0：1) 13 groups are amounted to
Plus 10 times of amount water, 30min is soaked, is boiled three times, each 1h, decoction liquor, filtered through gauze, merging filtrate, high speed is collected
Centrifuge after 10min, merge supernatant, use Rotary Evaporators concentrated extracting solution, be concentrated into 0.4g/ml containing crude drug amount.
2. method
2.1 diabetes animal models make
200 male SD rats, 190 are served only for modeling, 10 left blank groups.190 rats for being served only for modeling pass through
After adaptability cultivation in three days, give the high sugar of one week high fat and raise, after the high sugar of high fat is raised one week, give Rat Fast 12h, claim
Weight, Streptozotocin (configures) 45mg/kg disposable celiacs with 0.1mol/L citrate buffers and injected, and continues the high sugar of high fat
Feed raises 72h, raises and blood glucose is surveyed after 72h, it is diabetic animal models that fasting blood-glucose, which is more than 16.7mmol/L person, there is 162
Rat selects 150 into mould rat through examining into mould, is randomly divided into 15 groups, i.e. model group, positive group, A groups, B groups, C groups, D
Group, E groups, F groups, G groups, H groups, I groups, J groups, K groups, L groups, M groups.
2.2 experiment packets and dosage
Rats in test groups packet, dosage etc. are as shown in table 1 below：
Table 1：Experiment packet and dosage
Two, Testing index and method
Fasting blood-glucose：A rat fasting blood-glucose is surveyed weekly, is surveyed before blood glucose, is given after Rat Fast 12h, 12h, adopt each group
Rat tail vein blood, blood glucose is surveyed using blood glucose meter and blood sugar test paper.
It is administered after four weeks, puts to death mouse, sustainer takes blood, surveys serum insulin, free fatty, IL-6, TNF-α
Content.Method of testing, the method provided according to kit, is detected.
Three, experimental results
1. experimentation phenomenon
In experimentation, there is drinking a lot of water in model group, melbine group, A, B, C groups, and diet is more, and urination is more to be showed
As.Since second week, drug therapy each group is given, amount of drinking water urination amount has all been reduced.
2. blood glucose
Respectively at first week, second week the 3rd week, adopted each group rat tail vein blood, was surveyed using blood glucose meter and blood sugar test paper
Blood glucose, data such as table 2 below.
Table 2：Rat tail vein blood blood glucose test result
| Group | First week | Second week | 3rd week | 4th week |
| Blank group | 5.9167±0.46224** | 6.0667±0.50465** | 5.9167±0.46224** | 5.8833±0.29944** |
| Model group | 22.0667±3.30495 | 20.4833±1.77473 | 20.7167±1.95798 | 20.9677±1.50155 |
| Positive group | 7.5667±0.60553** | 7.3667±0.76333** | 7.4500±0.74498** | 7.8000±0.69570** |
| A groups | 11.0833±0.87044** | 10.2167±0.75476** | 9.5833±0.70261** | 9.5167±0.84479** |
| B groups | 11.1167±0.91524** | 10.1167±0.56362** | 9.5333±0.90480** | 9.1500±0.67355** |
| C groups | 11.3000±0.84143** | 10.5167±1.18729** | 9.5000±1.07517** | 9.8667±0.48580** |
| D groups | 19.2833±0.91305* | 19.8500±1.28335* | 18.4500±0.95237* | 17.4000±1.08995* |
| E groups | 19.4167±1.35708* | 19.6833±1.57787* | 18.4833±0.89981* | 17.4167±1.07036* |
| F groups | 18.9167±1.56386* | 19.4000±1.33116* | 18.5833±0.83526* | 17.6167±0.71671* |
| G groups | 19.8167±1.65459* | 19.9833±1.33479* | 18.4167±0.63692* | 17.6833±0.68240* |
| H groups | 19.8167±1.93847* | 19.6833±1.45247* | 18.4667±0.69186* | 17.6333±0.87101* |
| I groups | 19.9167±1.73253* | 19.6000±1.45190* | 18.3667±0.81158* | 17.5500±0.85264* |
| J groups | 19.5833±2.35917* | 19.6333±1.21600* | 18.4500±0.94181* | 17.6833±0.71110* |
| K groups | 19.7833±1.33928* | 19.6500±1.32023* | 18.7000±0.80747* | 17.5677±0.83347* |
| L groups | 20.0333±1.43481* | 19.8667±1.34710* | 18.6833±0.91305* | 17.6500±0.77910* |
| M groups | 19.4833±1.07595* | 19.7167±1.32577* | 18.4500±0.94181* | 17.8333±0.82624* |
Compared with model group, *：P ＜ 0.05, * *：P ＜ 0.01；
As shown in Table 1, model group is compared with blank group, blood glucose rise, and with significant difference (P ＜ 0.01).Give
The blood glucose of medicine each group is variant compared with model group, illustrates that the blood glucose that each group is administered has been reduced.Wherein, its middle-jiao yang, function of the spleen and stomach
Property group, tri- groups of hypoglycemic effects of A, B, C substantially, compared with model group, with significant difference (P ＜ 0.01), and the ratio maintained
It is more steady.
3. serum middle finger target is detected
(1) free fatty (FFA) is that aliphatic acid is transported to the tissue profit such as liver and muscle from the adipose tissue of storing position
Form, wherein most is water insoluble, can only be with that could be transported after albumin combination.Having proven to FFA increases can cause
IR. mechanism includes：Histiocytic insulin receptor quantity reduction, suppresses insulin and is combined with acceptor；Cause insulin
Receptor tyrosine kinase activity is abnormal, blocks insulin signaling transmission；Reduce the uptake and utilization of the glucose of insulin-mediated.
Weaken the inhibitory action that insulin is produced to glycogen, influence Glycogen synthesis, selectivity infringement target tissue glucose transport reduces pancreas
Island element secretion.
(2) insulin is a kind of proteohormone, the islet β cell in pancreas.Insulin participates in regulation sugared generation
Thank, control blood glucose balance, available for treatment diabetes.
(3) interleukin-6 (IL-6) is both inflammatory factor and a kind of Adipocyte Factor, and IL-6 can pass through a variety of machines
The sensitiveness of system influence insulin, causes the generation of insulin resistance and type II diabetes.
TNF (TNF-α) be activation mononuclear macrophage and fat cell secretion a kind of cell because
Son, not only plays an important role, and can induce insulin resistance in the generating process of fat and obesity-related disease.
As shown in table 3, accompanying drawing 1-4 is the data drawing list visualization exhibition of experimental result to indices testing result in serum
Show, including in rat blood serum free fatty, IL-6, TNF-α etc. content.
Table 3：Rat blood serum middle finger target is detected
| Packet | Free fatty (μm ol/L) | Serum insulin (mIU/L | IL-6(ng/mL) | TNF-α(ng/mL) |
| Blank group | 32.9867±4.75405** | 5.3033±0.25797* | 297.6283±8.72143* | 211.9767±5.62059** |
| Model group | 134.4867±13.52240 | 4.1783±0.48495 | 323.2183±5.20646 | 246.6900±6.09612 |
| Positive group | 85.3200±11.98040** | 6.1867±0.32556** | 301.0217±4.85804** | 185.9450±12.13704** |
| A | 96.9583±9.33107** | 5.6067±0.50449* | 306.1400±4.46169* | 219.3367±9.05011* |
| B | 96.9450±5.80864** | 5.6317±0.46791* | 304.7967±6.21702* | 218.2533±6.29955* |
| C | 95.0250±7.19724** | 5.6017±0.38160* | 305.2017±4.79235* | 220.4050±7.02966* |
| D | 104.8100±7.58457* | 5.1467±0.40357* | 307.6400±2.82677* | 228.4667±5.44720* |
| E | 100.9467±7.40685* | 5.2467±0.14123* | 307.7233±4.74565* | 222.1683±7.72281* |
| F | 100.5633±7.82240* | 5.0967±0.29629* | 308.0917±2.71480* | 223.0850±7.41857* |
| G | 107.5150±2.96830* | 5.2150±0.33537* | 307.1167±3.13387* | 224.9850±7.31330* |
| H | 100.9033±7.51210* | 5.1433±0.27983* | 307.7200±4.47686* | 223.4733±9.21052* |
| I | 103.4267±5.38369* | 5.2317±0.36575* | 308.7817±2.56338* | 223.3150±6.45106* |
| J | 100.8517±7.87618* | 5.1233±0.33661* | 309.5833±5.56605* | 219.3200±7.75152* |
| K | 102.6250±5.89335* | 5.2600±0.30430* | 308.4417±3.70296* | 220.6950±6.89252* |
| L | 101.1405±7.32764* | 5.1950±0.33548* | 307.2000±3.85508* | 221.9800±6.76688* |
| M | 100.2967±9.39779* | 5.2133±0.21160* | 308.6617±3.16650* | 223.2450±6.84869* |
Compared with model group, *：P ＜ 0.05, * *：P ＜ 0.01；
As shown in Table 3：
Model group is compared with blank group, the rise of serum free fatty acid concentration, and with significant difference (P ＜
0.01), the free fatty of administration each group is reduced, and has otherness compared with model group.Wherein, its positives group, A, B, C
Three groups of free fatty has significant difference (P ＜ 0.01) compared with model group.
Model group is compared with blank group, and the serum insulin liter of each group is administered in serum insulin reduction (P ＜ 0.05)
Height, has difference compared with model group.Wherein, its positives group of serum insulin has significant difference (P compared with model group
＜ 0.01), remaining administration each group is compared with model group, with otherness (P<0.05).
Model group is compared with blank group, blood serum IL-6 content rise (P<0.01), administration each group can be reduced in rat blood serum
IL-6 concentration, compared with model group have difference.Its positives group of blood serum IL-6 content is compared with model group, with notable
Sex differernce (P ＜ 0.01), remaining administration each group is compared with model group, with otherness (P<0.05).
Model group is compared with blank group, TNF-α content rise (P<0.01), administration each group can be reduced in rat blood serum
The concentration of TNF-α, its positives group compared with model group, with significant difference (P ＜ 0.01), remaining administration each group and model
Group is compared, with otherness (P<0.05).
Free fatty in size and serum by analyzing each group blood glucose value, insulin, IL-6 and TNF- Α contain
Amount, it is known that, the blood sugar decreasing effect after sealwort mulberry leaf radix polygonati officinalis three's compatibility is better than simple and two-by-two compatibility.
4th, analysis is discussed
The mechanism of action of STZ induced diabetes is to produce substantial amounts of oxide by inducing, and destroys the DNA of beta Cell of islet,
Beta Cell of islet necrosis and insulin secretion is caused to reduce and induced Diabetic.And treating diabetes strategy be mainly it is hypoglycemic and
Recover insulin level.
Experimental result shows that the sealwort of melbine and different proportion, mulberry leaf, the decocting liquid of radix polygonati officinalis have to diabetes rat
Notable blood sugar decreasing effect, and serum free fatty acid is reduced, interleukin-6, the content of TNF increases serum
The content of insulin.Thus infer, the sealwort of melbine and different proportion, mulberry leaf, the decocting liquid of radix polygonati officinalis, it may be possible to pass through drop
The interleukin-6 of free fatty in low blood, TNF concentration mitigates its suppression to insulin secretion, improves pancreas islet
β cell functions, reduce blood glucose.Its deeper mechanisms needs further research.
In the sealwort of different ratio, mulberry leaf, radix polygonati officinalis each group, tri- groups of blood glucose of A, B, C and free fatty and interleukin-
6 concentration has significant difference with model group, and in experimentation, tri- groups of A, B, C relative to other each groups, amount of drinking water with
Urination amount is significantly reduced.The traditional Chinese medical science thinks, the deficiency of vital energy is the cause of disease of diabetes, and it is the main pathogenesis of diabetes that the deficiency of Yin is scorching.QI invigorating is grown
Cloudy heat-clearing is then this sick basic therapy.Soil is mended in sealwort boosting qi and nourishing yin, invigorating the spleen, to give birth to lung gold；Mulberry leaf clearing away the lungheat and moisturizing, to give birth to kidney
Water；Radix polygonati officinalis nourishing yin to moisten dryness is to promote the production of body fluid to quench thirst.Infer and used because three medicines are same, supplementing qi and nourishing yin, moistening lung and production of body fluid, the dirty all tune of spleen and lung kidney three,
So that the card of deficiency of both qi and yin disappears certainly.Therefore sealwort, radix polygonati officinalis, the compound of mulberry leaf meet the criterion of TCM treatment of diabetes, and in fact
Test result also demonstrate that, three is with using, and hypoglycemic effect is best.
Claims (10)
1. a kind of traditional Chinese medicinal composition with effect of reducing blood sugar, including following parts by weight traditional Chinese medicine ingredients composition：4-10 parts of sealwort：0-3 parts of radix polygonati officinalis：
0-3 parts of mulberry leaf.
2. traditional Chinese medicinal composition with effect of reducing blood sugar as claimed in claim 1, it is characterised in that the weight of each composition in the Chinese medicine composition
Measuring part ratio is：4-8 parts of sealwort：1-2 parts of radix polygonati officinalis：1-2 parts of mulberry leaf.
3. traditional Chinese medicinal composition with effect of reducing blood sugar as claimed in claim 2, it is characterised in that the weight of each composition in the Chinese medicine composition
Measuring part ratio is：2-4 parts of sealwort：1 part of radix polygonati officinalis：1 part of mulberry leaf.
4. a kind of method for preparing above-mentioned Chinese medicine composition, comprises the following steps：
（1）Sealwort, radix polygonati officinalis and mulberry leaf, chopping or section are taken, 1-20 times of water is added, immersion is decocted 1-5 times, each 0.1-3 is small
When；
（2）Collect decoction liquor, filtering, merging filtrate；Centrifugation, merges supernatant, is concentrated into more than 0.2g/mL containing crude drug amount.
5. preparation method as claimed in claim 4 is characterized in that, in step 1, chopping to maximum gauge is less than 2-5mm, or
It is sliced into thickness 1-4mm piece.
6. preparation method as claimed in claim 4 is characterized in that, in step 1, add 5-12 times of water immersion.
7. the preparation method as described in claim 4 or 6 is characterized in that, in step 1, soak time 5-60min.
8. preparation method as claimed in claim 4 is characterized in that, in step 1, decoct number of times 2-4 times.
9. preparation method as claimed in claim 4 is characterized in that, in step 2, filtered through gauze during filtering.
10. preparation method as claimed in claim 4 is characterized in that, in step 2, concentrated in concentration process using rotary evaporation,
To more than 0.4g/mL containing crude drug amount.
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| CN108562685A (en) * | 2018-06-26 | 2018-09-21 | 成都中医药大学 | A kind of sealwort, mulberry leaf and radix polygonati officinalis compatibe drug composition quality detection method |
| CN108783155A (en) * | 2018-06-26 | 2018-11-13 | 成都中医药大学 | A kind of sealwort, mulberry leaf, radix polygonati officinalis compatibility beverage and preparation method thereof |
| CN111821380A (en) * | 2020-07-31 | 2020-10-27 | 深圳市清生元生物技术股份有限公司 | Hypoglycemic composition and preparation method and application thereof |
| CN112640979A (en) * | 2021-01-07 | 2021-04-13 | 北京善养健康科技有限公司 | Health tea for conditioning chronic diseases and preparation method thereof |
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| CN114431384A (en) * | 2022-01-27 | 2022-05-06 | 四川省川北人家农业科技有限公司 | Mulberry leaf and sealwort instant beverage and preparation method thereof |
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| CN108562685A (en) * | 2018-06-26 | 2018-09-21 | 成都中医药大学 | A kind of sealwort, mulberry leaf and radix polygonati officinalis compatibe drug composition quality detection method |
| CN108783155A (en) * | 2018-06-26 | 2018-11-13 | 成都中医药大学 | A kind of sealwort, mulberry leaf, radix polygonati officinalis compatibility beverage and preparation method thereof |
| CN108562685B (en) * | 2018-06-26 | 2020-05-12 | 成都中医药大学 | Quality detection method for rhizoma polygonati, folium mori and radix polygonati officinalis combined pharmaceutical composition |
| CN111821380A (en) * | 2020-07-31 | 2020-10-27 | 深圳市清生元生物技术股份有限公司 | Hypoglycemic composition and preparation method and application thereof |
| CN112640979A (en) * | 2021-01-07 | 2021-04-13 | 北京善养健康科技有限公司 | Health tea for conditioning chronic diseases and preparation method thereof |
| CN114306544A (en) * | 2022-01-25 | 2022-04-12 | 海南中食肽科生物科技有限公司 | Blood sugar reducing composition and preparation method thereof |
| CN114306544B (en) * | 2022-01-25 | 2023-08-11 | 海南中食肽科生物科技有限公司 | Hypoglycemic composition and preparation method thereof |
| CN114431384A (en) * | 2022-01-27 | 2022-05-06 | 四川省川北人家农业科技有限公司 | Mulberry leaf and sealwort instant beverage and preparation method thereof |
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