CN106970078A - A kind of test paper and its preparation and detection method for detecting OPA - Google Patents
A kind of test paper and its preparation and detection method for detecting OPA Download PDFInfo
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Abstract
The invention belongs to disinfectant detection technique field, it is related to a kind of test paper and its preparation and detection method for detecting OPA.Described test paper is fixed with colored indicator on plain paper, the one or more combinations of described colored indicator in amino acid esters compound, arylamine and/or fat primary amine.Utilize the test paper and its preparation and detection method of the detection OPA of the present invention, the test paper prepared can be made in O-phthalic aldehyde concentration in detecting thimerosal, reduce dissolving of the colored indicator fixed on test paper in thimerosal, so that OPA Concentration Testing result is influenceed small by test paper with thimerosal time of contact, developing time, make testing result more true and reliable.
Description
Technical field
The invention belongs to disinfectant detection technique field, it is related to a kind of test paper for detecting OPA and its preparation and inspection
Survey method.
Background technology
OPA (O-phthalaldehyde, OPA) is a kind of conventional disinfectant, with property stabilization, sterilization
Spectrum is wide, sterilizing power is strong and to skin and mucosa it is nonirritant, to metal low-corrosiveness the advantages of, therefore in medicine equipment cold sterilization
Field is widely used.To ensure that the valid density of OPA in Disinfection Effect, regulation thimerosal is more than 0.3%
(m/v) it can be used when.
For that can make O-phthalaldehyde disinfectant product in market surpervision and routine use by quick detection, recent year
Outside all in Concentration Testing test paper of the research and development for O-phthalaldehyde disinfectant product, existing test paper is broadly divided into two classes.
The Cleaning Principle of first kind Test paper is as follows.Sodium sulfite generates alkali with O-phthalic aldehyde reaction, makes reactant
It is pH value rise；After being reacted again by a certain amount of carboxylic group introduced on test paper with alkali, phenolphthalein is indicated by remaining alkali
Agent discoloration carrys out OPA concentration range in judgement sample.The shortcoming of this kind of test paper has two, and one is that pH value can be with before and after reaction
The change of disinfectant pH value itself and change, influence the color change of indicator, cause measurement inaccurate；Two be if O-phthalic
There is buffer system in aldehyde disinfectant, then the test paper will completely be examined and not measure OPA.
The Cleaning Principle of Equations of The Second Kind Test paper is as follows.Utilize aldehydes and amino acids reaction generation yellow substance
Afterwards, the concentration range measure for carrying out OPA is compareed with standard color comparison card.The shortcoming of this kind of test paper be pick it is detected
Partial amino-acid class material can be quickly soluble in disinfectant solution during solution, caused amino to be lost in, caused test paper color development to shoal,
So that OPA is quantitative inaccurate.
The content of the invention
The primary and foremost purpose of the present invention is to provide a kind of test paper for detecting OPA, can make the test paper prepared
In O-phthalic aldehyde concentration in detecting thimerosal, dissolving of the colored indicator fixed on test paper in thimerosal is reduced, from
And OPA Concentration Testing result is influenceed small by test paper and thimerosal time of contact, developing time, make testing result more
Plus it is true and reliable.
In order to achieve this, in the embodiment on basis, the present invention provides a kind of test paper for detecting OPA, institute
The test paper stated is fixed with colored indicator on plain paper, described colored indicator be selected from amino acid esters compound, arylamine and/
Or one or more combinations in fat primary amine.
Aldehyde radical of the invention based on OPA can generate yellow Schiff with the amino condensation of amino acid esters compound
The principle of alkali is carried out, and the shade shown by product on test paper is compareed with standard color comparison card, judges OPA
Concentration range.
In a preferred embodiment, the present invention provides a kind of test paper for detecting OPA, wherein described
Amino acid esters compound is selected from glycine methyl ester, glycine ethyl ester, lysine methyl ester, ethyl ester of lysine, threonine methyl, Soviet Union
Propylhomoserin ethyl ester, tryptophan methyl ester, tryptophan ethyl ester, histidine methylester, histidine ethyl ester, the different monooctyl ester of glycine, glycine 12
One or more combinations in ester, phenyalanine methyl ester and their salt；
Described arylamine, fat primary amine are one or more in open-chain crown ether, dodecyl polyaniline, octyl amine
Combination.
In a preferred embodiment, the present invention provides a kind of test paper for detecting OPA, wherein every institute
The total amount of described amino acid esters compound, arylamine and/or fat primary amine is 0.2-16mg on the test paper stated.
In a kind of more preferred embodiment, the present invention provides a kind of test paper for detecting OPA, wherein often
The total amount of described amino acid esters compound, arylamine and/or fat primary amine is 0.5-5mg on Zhang Suoshu test paper.
Second object of the present invention is to provide the preparation method of the test paper of foregoing detection OPA, can make system
Standby obtained test paper reduces colored indicator fixed on test paper in thimerosal in O-phthalic aldehyde concentration in detecting thimerosal
In dissolving so that OPA Concentration Testing result is influenceed small by test paper with thimerosal time of contact, developing time, make
Testing result is more true and reliable.
In order to achieve this, in the embodiment on basis, the present invention provides the test paper of foregoing detection OPA
Preparation method, comprises the following steps：
(1) plain paper is prepared：Mass volume ratio concentration 0.1%-20% fixative solution is prepared, a certain size will be cut into
Filter paper immerse in the solution, fully infiltration after, in 50-90 DEG C drying；
(2) preparation of solution A：Adsorbent containing mass volume ratio concentration 0.2%-4% in solution A, 0.2%-16%
Colored indicator, 0.02%-5% suspension dispersive agent stirs；
(3) the plain paper for preparing step (1) is immersed in solution A, fully infiltration, in 50-90 DEG C of drying；
(4) preparation of B solution：Adsorbent containing mass volume ratio concentration 0.2%-6% in B solution, 0.1%-6%'s
Suspension dispersive agent, 0.2%-10% adhesive, stirs；
(5) paper for preparing step (3) is immersed in B solution, fully infiltration, in 50-90 DEG C of drying；
(6) the paper cutouts strip for preparing step (5), pastes on the ground not absorbed water, is cut into test-strips
(for example 0.3-1cm is wide), that is, be made OPA quick detection test paper.
In a preferred embodiment, the present invention provides the preparation method of the test paper of foregoing detection OPA,
Wherein described fixative is water-soluble high-molecular material, selected from carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose
And one or more combinations in their metal salt.
In a preferred embodiment, the present invention provides the preparation method of the test paper of foregoing detection OPA,
The mass volume ratio concentration for the described fixative solution wherein prepared in step (1) is 0.5-5%.
In a preferred embodiment, the present invention provides the preparation method of the test paper of foregoing detection OPA,
Wherein described adsorbent is white or colourless adsorbent, selected from white carbon, allkyl methacrylate cross-linked polymer
(ALLYL METHACRYLATES CROSSPOLYMER, commercially produced product is for example) or poly- (metering system E200
Sour lauryl -co- ethylene glycol dimethacrylate) (Poly (lauryl methacrylate-co-ethylene glycol
Dimethacrylate), commercially produced product is for example6603) one or more combinations in.
In a preferred embodiment, the present invention provides the preparation method of the test paper of foregoing detection OPA,
The mass volume ratio concentration of adsorbent is 0.2- in the described solution A and B solution wherein prepared in step (2) and step (4)
2%.
In a preferred embodiment, the present invention provides the preparation method of the test paper of foregoing detection OPA,
The mass volume ratio concentration of colored indicator is 0.5-5% in the described solution A wherein prepared in step (2).
In a preferred embodiment, the present invention provides the preparation method of the test paper of foregoing detection OPA,
Wherein described suspension dispersive agent is anion, nonionic and/or amphion dispersant, selected from iso-octyl benzene sulfonic acid sodium salt, ten
One or more combinations in dialkyl benzene sulfonic acids sodium, APES, CAB.
In a preferred embodiment, the present invention provides the preparation method of the test paper of foregoing detection OPA,
The mass volume ratio concentration of suspension dispersive agent is 0.05-1% in the described solution A wherein prepared in step (2)；Step (4)
The mass volume ratio concentration of suspension dispersive agent is 0.1-2% in the described B solution of middle preparation.
In a preferred embodiment, the present invention provides the preparation method of the test paper of foregoing detection OPA,
Wherein described adhesive is the water-soluble high-molecular material with bond properties, selected from polyvinyl alcohol and/or polyethylene glycol.
In a preferred embodiment, the present invention provides the preparation method of the test paper of foregoing detection OPA,
The mass volume ratio concentration of adhesive is 0.5-5% in the described B solution wherein prepared in step (4).
The test paper that third object of the present invention is to provide foregoing detection OPA is used for O-phthalic aldehyde concentration
Detection method, so that the test paper prepared can be made in O-phthalic aldehyde concentration in detecting thimerosal, is reduced fixed on test paper
Dissolving of the colored indicator in thimerosal so that when OPA Concentration Testing result is contacted by test paper with thimerosal
Between, developing time influence it is small, make testing result more true and reliable.
In order to achieve this, in the embodiment on basis, the test paper that the present invention provides foregoing detection OPA is used
In the detection method of O-phthalic aldehyde concentration, described detection method in turn includes the following steps：
(1) described test paper is immersed in solution to be measured and carries out haptoreaction；
(2) the haptoreaction time (such as 2 seconds) after takes out solution to be measured unnecessary on described test paper, removal test paper；
(3) horizontal positioned test paper makes it develop the color naturally, is compareed with standard color comparison card, obtains the concentration of OPA.
In a preferred embodiment, the present invention provides the test paper of foregoing detection OPA for O-phthalic
The haptoreaction time described in the detection method of aldehyde concentration, wherein step (1) is 2-4s；Showing naturally described in step (3)
The color time is 1-5min.
The overall beneficial effect of the present invention is, utilizes the test paper for detecting OPA of the present invention and its preparation and inspection
Survey method, can make the test paper prepared in O-phthalic aldehyde concentration in detecting thimerosal, reduce fixed on test paper show
Dissolving of the color indicator in thimerosal so that OPA Concentration Testing result by test paper and thimerosal time of contact,
Developing time influence is small, makes testing result more true and reliable.
Specific beneficial effect of the invention embodies as follows：
1st, the present invention can detect the mass volume ratio concentration range of O-phthalaldehyde disinfectant for 0.3%-0.6% (when
When OPA mass volume ratio concentration is in the range of 0.3%-0.6%, test paper shows uniform yellow；When OPA matter
When measuring volume by volume concentration less than 0.3%, test paper shows faint yellow or not developed the color), the depth changed colour according to test paper judges O-phthalic
The concentration range of aldehyde.It is various amino acid to be used in the present invention on Test paper with the primary raw material of OPA reaction solution
Ester, for using amino acids compared to traditional colour developing test paper for raw material, the amino acid esters used in the present invention
Rate of dissolution is low in aqueous for raw material, and compound when picking tested liquid on test paper spreads relatively slowly into water, particularly
With after aldehyde reaction turn into water-insoluble acetal, it is ensured that the amino quantity on test paper so that OPA can be therewith
Fully reaction, colour developing is easier to differentiate, quantitative more accurate.
2nd, the different chains of certain amino acid may be selected in the amino acid esters raw material range of choice used in the present invention extensively, both
Long esters, such as glycine methyl ester, glycine ethyl ester, histidine methylester, histidine ethyl ester etc.；Also different aminoacids kind may be selected
The carboxylate of class, such as ethyl ester of lysine, leucinethylester etc..In selection, can simultaneously from the condition of reaction, raw material into
Originally, selection optimum feed stock is considered in terms of reaction time speed.
3rd, the present invention can avoid the test paper caused by picking the difference of time when operation from showing color difference.Traditional test paper
When testing same O-phthalaldehyde disinfectant, if picking, the time is slightly long, and the amino acids material of attachment is lost in and can increased, and takes out examination
Developed the color after paper slightly shallow；If it is relatively short to pick the time, develop the color relatively slightly deep, this can cause same fluid to be measured to be difficult to accurately sentence
Its disconnected concentration.Solubility is low in the amino-acid ester compound aqueous solution used in the present invention, effectively prevent temporal because picking
The shade that difference is caused differs, and tester is easier to judge the concentration of OPA, easy to operation.
Embodiment
The embodiment to the present invention is further illustrated with reference to embodiments, but the embodiment party of the present invention
Formula is not limited to examples below.The concentration hereinafter occurred, as being that mass volume ratio (m/v) is dense without particularly pointing out
Degree.
Embodiment 1：The preparation (one) of O-phthalaldehyde disinfectant Concentration Testing test paper
O-phthalaldehyde disinfectant Concentration Testing test paper is prepared as follows.
1st, plain paper is prepared：First prepare 0.5% fixative solution.Compound method：5g carboxymethyl celluloses are weighed, by it
It is dissolved in deionized water, is settled to 1000mL.Filter paper is cut into the size of 10cm × 10cm sizes, immersed in the solution, fully
After infiltration, in 50 DEG C of drying.
2nd, the preparation of solution A：Contain 0.2% adsorbent, 0.5% colored indicator, 0.05% suspended dispersed in solution A
Agent.Compound method is：1g white carbons, 1.56g glycine methyl ester hydrochlorides, 0.94g histidine methylesters are added into deionized water
Hydrochloride, 0.25g APESs, 500mL is settled to deionized water, is stirred.
3rd, the plain paper prepared in the 1st step is immersed in solution A, fully infiltration, in 50 DEG C of drying.
4th, the preparation of B solution：Water-soluble binder containing 0.2% adsorbent and 0.5% in B solution, and 0.1% is outstanding
Floating dispersant.Compound method is：1g white carbons are added into deionized water, 50g concentration is 5% polyvinyl alcohol water solution,
0.5g APESs, 500mL is settled to deionized water, is stirred.
5th, the paper prepared in the 3rd step is immersed in B solution, fully infiltration, in 50 DEG C of drying.
6th, test paper is cut into the wide strips of 1cm, pasted on the ground not absorbed water, be cut into the wide test-strips of 1cm, that is, make
Into OPA quick detection test paper card.
The mass percentage content of colored indicator is 0.5mg in the test card prepared as stated above.
Embodiment 2：The preparation (two) of O-phthalaldehyde disinfectant Concentration Testing test paper
O-phthalaldehyde disinfectant Concentration Testing test paper is prepared as follows.
1st, plain paper is prepared：First prepare 5% fixative solution.Compound method：50g hydroxyethyl celluloses are weighed, by it
It is dissolved in deionized water, is settled to 1000mL.Filter paper is cut into the size of 10cm × 10cm sizes, immersed in the solution, fully
After infiltration, in 90 DEG C of drying.
2nd, the preparation of solution A：Contain 2% adsorbent, 5% colored indicator, 1% suspension dispersive agent in solution A.Preparation side
Method is：10g white carbons, 12.5g lysine methyl ester hydrochlorides, 12.5g threonine ethyl hydrochlorides, 5g are added into deionized water
Neopelex, 500mL is settled to deionized water, is stirred.
3rd, the plain paper prepared in the 1st step is immersed in solution A, fully infiltration, in 90 DEG C of drying.
4th, the preparation of B solution：Water-soluble binder containing 2% adsorbent and 5% in B solution, and 2% suspended dispersed
Agent.Compound method is：10g white carbons are added into deionized water, 125g concentration is 20% Aqueous Solutions of Polyethylene Glycol, 10g ten
Dialkyl benzene sulfonic acids sodium, 500mL is settled to deionized water, is stirred.
5th, the paper prepared in the 3rd step is immersed in B solution, fully infiltration, in 90 DEG C of drying.
6th, test paper is cut into the wide strips of 1cm, pasted on the ground not absorbed water, be cut into the wide test-strips of 1cm, that is, make
Into OPA quick detection test paper card.
The mass percentage content of colored indicator is 5mg in the test card prepared as stated above.
Embodiment 3：The preparation (three) of O-phthalaldehyde disinfectant Concentration Testing test paper
O-phthalaldehyde disinfectant Concentration Testing test paper is prepared as follows.
1st, plain paper is prepared：First prepare 0.5% fixative solution.Compound method：5g carboxymethyl celluloses are weighed, by it
It is dissolved in deionized water, is settled to 1000mL.Filter paper is cut into the size of 10cm × 10cm sizes, immersed in the solution, fully
After infiltration, in 50 DEG C of drying.
2nd, the preparation of solution A：Contain 0.2% adsorbent, 0.5% colored indicator, 0.05% suspended dispersed in solution A
Agent.Compound method is：1g is added into deionized waterE200,1.56g tryptophan methyl ester hydrochloride, 0.94g
Tryptophan carbethoxy hydrochloride, 0.25g iso-octyl benzene sulfonic acid sodium salts, is settled to 500mL with deionized water, stirs.
3rd, the plain paper prepared in the 1st step is immersed in solution A, fully infiltration, in 50 DEG C of drying.
4th, the preparation of B solution：Water-soluble binder containing 0.2% adsorbent and 0.5% in B solution, and 0.1% is outstanding
Floating dispersant.Compound method is：1g is added into deionized waterE200,50g concentration are 5% polyvinyl alcohol
The aqueous solution, 0.5g iso-octyl benzene sulfonic acid sodium salts, is settled to 500mL with deionized water, stirs.
5th, the paper prepared in the 3rd step is immersed in B solution, fully infiltration, in 50 DEG C of drying.
6th, test paper is cut into the wide strips of 1cm, pasted on the ground not absorbed water, be cut into the wide test-strips of 1cm, that is, make
Into OPA quick detection test paper card.
The mass percentage content of colored indicator is 0.5mg in the test card prepared as stated above.
Embodiment 4：The preparation (four) of O-phthalaldehyde disinfectant Concentration Testing test paper
O-phthalaldehyde disinfectant Concentration Testing test paper is prepared as follows.
1st, plain paper is prepared：First prepare 5% fixative solution.Compound method：50g hydroxyethyl celluloses are weighed, by it
It is dissolved in deionized water, is settled to 1000mL.Filter paper is cut into the size of 10cm × 10cm sizes, immersed in the solution, fully
After infiltration, in 90 DEG C of drying.
2nd, the preparation of solution A：Contain 2% adsorbent, 5% colored indicator, 1% suspension dispersive agent in solution A.Preparation side
Method is：10g is added into deionized water6603,12.5g glycine methyl esters, 12.5g glycine ethyl esters, 5g 12
Sodium alkyl benzene sulfonate, 500mL is settled to deionized water, is stirred.
3rd, the plain paper prepared in the 1st step is immersed in solution A, fully infiltration, in 90 DEG C of drying.
4th, the preparation of B solution：Water-soluble binder containing 2% adsorbent and 5% in B solution, and 2% suspended dispersed
Agent.Compound method is：10g is added into deionized water6603,125g concentration are water-soluble for 20% polyethylene glycol
Liquid, 10g neopelexes, 500mL is settled to deionized water, is stirred.
5th, the paper prepared in the 3rd step is immersed in B solution, fully infiltration, in 90 DEG C of drying.
6th, test paper is cut into the wide strips of 1cm, pasted on the ground not absorbed water, be cut into the wide test-strips of 1cm, that is, make
Into OPA quick detection test paper card.
The mass percentage content of colored indicator is 5mg in the test card prepared as stated above.
Embodiment 5：The preparation (five) of O-phthalaldehyde disinfectant Concentration Testing test paper
O-phthalaldehyde disinfectant Concentration Testing test paper is prepared as follows.
1st, plain paper is prepared：First prepare 2% fixative solution.Compound method：20g hydroxypropyl celluloses are weighed, by it
It is dissolved in deionized water, is settled to 1000mL.Filter paper is cut into the size of 10cm × 10cm sizes, immersed in the solution, fully
After infiltration, in 85 DEG C of drying.
2nd, the preparation of solution A：Contain 0.5% adsorbent, 3% colored indicator, 0.3% suspension dispersive agent in solution A.Match somebody with somebody
Method processed is：2.5g white carbons, 10g open-chain crown ethers, 5g dodecyl polyanilines, 1.5g propyl group beets are added into deionized water
Alkali, 500mL is settled to deionized water, is stirred.
3rd, the plain paper prepared in the 1st step is immersed in solution A, fully infiltration, in 85 DEG C of drying.
4th, the preparation of B solution：Water-soluble binder containing 0.5% adsorbent and 2% in B solution, and 0.5% suspension
Dispersant.Compound method is：2.5g white carbons are added into deionized water, 50g concentration is 20% polyvinyl alcohol water solution,
2.5g CABs, 500mL is settled to deionized water, is stirred.
5th, the paper prepared in the 3rd step is immersed in B solution, fully infiltration, in 85 DEG C of drying.
6th, test paper is cut into the wide strips of 1cm, pasted on the ground not absorbed water, be cut into the wide test-strips of 1cm, that is, make
Into OPA quick detection test paper card.
The mass percentage content of colored indicator is 3mg in the test card prepared as stated above.
Embodiment 6：The preparation (six, reference examples) of O-phthalaldehyde disinfectant Concentration Testing test paper
O-phthalaldehyde disinfectant Concentration Testing test paper is prepared as follows.
1st, plain paper is prepared：First prepare 0.5% fixative solution.Compound method：5g carboxymethyl celluloses are weighed, by it
It is dissolved in deionized water, is settled to 1000mL.Filter paper is cut into the size of 10cm × 10cm sizes, immersed in the solution, fully
After infiltration, in 50 DEG C of drying.
2nd, the preparation of solution A：Contain 0.2% adsorbent, 0.5% colored indicator, 0.05% suspended dispersed in solution A
Agent.Compound method is：1g white carbons, 1.56g glycine, 0.94g histidines, 0.25g alkyl phenol polyoxies are added into ionized water
Vinethene, 500mL is settled to deionized water, is stirred.
3rd, the plain paper prepared in the 1st step is immersed in solution A, fully infiltration, in 50 DEG C of drying.
4th, the preparation of B solution：Water-soluble binder containing 0.2% adsorbent and 0.5% in B solution, and 0.1% is outstanding
Floating dispersant.Compound method is：1g white carbons are added into deionized water, 50g concentration is 5% polyvinyl alcohol water solution,
0.5g APESs, 500mL is settled to deionized water, is stirred.
5th, the paper prepared in the 3rd step is immersed in B solution, fully infiltration, in 50 DEG C of drying.
6th, test paper is cut into the wide strips of 1cm, pasted on the ground not absorbed water, be cut into the wide test-strips of 1cm, that is, make
Into OPA quick detection test paper card.
The mass percentage content of colored indicator is 0.5mg in the test card prepared as stated above.
Embodiment 7：The preparation (seven, reference examples) of O-phthalaldehyde disinfectant Concentration Testing test paper
O-phthalaldehyde disinfectant Concentration Testing test paper is prepared as follows.
1st, plain paper is prepared：First prepare 5% fixative solution.Compound method：50g hydroxyethyl celluloses are weighed, by it
It is dissolved in deionized water, is settled to 1000mL.Filter paper is cut into the size of 10cm × 10cm sizes, immersed in the solution, fully
After infiltration, in 90 DEG C of drying.
2nd, the preparation of solution A：Contain 2% adsorbent, 5% colored indicator, 1% suspension dispersive agent in solution A.Preparation side
Method is：10g white carbons are added into deionized water, 12.5g lysines, 12.5g threonines, 5g neopelexes are used
Deionized water is settled to 500mL, stir.
3rd, the plain paper prepared in the 1st step is immersed in solution A, fully infiltration, in 90 DEG C of drying.
4th, the preparation of B solution：Water-soluble binder containing 2% adsorbent and 5% in B solution, and 2% suspended dispersed
Agent.Compound method is：10g white carbons are added into deionized water, 125g concentration is 20% Aqueous Solutions of Polyethylene Glycol, 10g ten
Dialkyl benzene sulfonic acids sodium, 500mL is settled to deionized water, is stirred.
5th, the paper prepared in the 3rd step is immersed in B solution, fully infiltration, in 90 DEG C of drying.
6th, test paper is cut into the wide strips of 1cm, pasted on the ground not absorbed water, be cut into the wide test-strips of 1cm, that is, make
Into OPA quick detection test paper card.
The mass percentage content of colored indicator is 5mg in the test card prepared as stated above.
Embodiment 8：Detection of the O-phthalaldehyde disinfectant Concentration Testing test paper to O-phthalic aldehyde concentration in disinfectant
Detection of the Test paper of embodiment 1-7 preparations to O-phthalic aldehyde concentration in disinfectant is carried out as follows,
Wherein disinfectant is solution known to the O-phthalic aldehyde concentration prepared with OPA standard items.Specific testing conditions and inspection
Survey the results are shown in Table 1.
(1) the embodiment 1-7 Test papers prepared are immersed in solution to be measured known to O-phthalic aldehyde concentration and carried out
Haptoreaction；
(2) the haptoreaction time takes out described test paper after, removes solution to be measured unnecessary on test paper and (wipes the test paper back of the body off
The unnecessary solution to be measured in face)；
(3) horizontal positioned test paper makes it develop the color naturally (display yellow), is compareed with standard color comparison card, reads OPA
Concentration.
Condition and result of the Test paper of table 1 to OPA Concentration Testing in disinfectant
Claims (10)
1. a kind of test paper for detecting OPA, it is characterised in that described test paper is fixed with colored indicator on plain paper,
One or more combinations of the described colored indicator in amino acid esters compound, arylamine and/or fat primary amine.
2. test paper according to claim 1, it is characterised in that：Described amino acid esters compound is selected from glycine first
Ester, glycine ethyl ester, lysine methyl ester, ethyl ester of lysine, threonine methyl, threonine ethyl, tryptophan methyl ester, tryptophan second
In ester, histidine methylester, histidine ethyl ester, the different monooctyl ester of glycine, the diester of glycine ten, phenyalanine methyl ester and their salt
One or more combinations；
One or more groups of the described arylamine, fat primary amine in open-chain crown ether, dodecyl polyaniline, octyl amine
Close.
3. test paper according to claim 1, it is characterised in that：Described amino acid esters chemical combination on test paper described in every
The total amount of thing, arylamine and/or fat primary amine is 0.2-16mg.
4. the preparation method of the test paper according to claim 1-3 any one, it is characterised in that comprise the following steps：
(1) plain paper is prepared：Mass volume ratio concentration 0.1%-20% fixative solution is prepared, a certain size filter will be cut into
Paper is immersed in the solution, fully after infiltration, in 50-90 DEG C of drying；
(2) preparation of solution A：Adsorbent containing mass volume ratio concentration 0.2%-4% in solution A, 0.2%-16%'s is aobvious
Color indicator, 0.02%-5% suspension dispersive agent, stirs；
(3) the plain paper for preparing step (1) is immersed in solution A, fully infiltration, in 50-90 DEG C of drying；
(4) preparation of B solution：Adsorbent containing mass volume ratio concentration 0.2%-6% in B solution, 0.1%-6% suspension
Dispersant, 0.2%-10% adhesive, stirs；
(5) paper for preparing step (3) is immersed in B solution, fully infiltration, in 50-90 DEG C of drying；
(6) the paper cutouts strip for preparing step (5), is pasted on the ground not absorbed water, and is cut into test-strips, that is, is made
Into OPA quick detection test paper.
5. preparation method according to claim 4, it is characterised in that：Described fixative is water-soluble high-molecular material,
One or more combinations in carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose and their metal salt.
6. preparation method according to claim 4, it is characterised in that：Described adsorbent is white or colourless absorption
Agent, selected from white carbon, allkyl methacrylate cross-linked polymer or poly- (lauryl methacrylate -co- glycol dinitrate
Base acrylate) in one or more combinations.
7. preparation method according to claim 4, it is characterised in that：Described suspension dispersive agent is anion, nonionic
And/or amphion dispersant, selected from iso-octyl benzene sulfonic acid sodium salt, neopelex, APES, propyl group
One or more combinations in glycine betaine.
8. preparation method according to claim 4, it is characterised in that：Described adhesive is with the water-soluble of bond properties
Property high polymer material, selected from polyvinyl alcohol and/or polyethylene glycol.
9. the test paper according to claim 1-3 any one is used for the detection method of O-phthalic aldehyde concentration, its feature exists
In described detection method in turn includes the following steps：
(1) described test paper is immersed in solution to be measured and carries out haptoreaction；
(2) the haptoreaction time takes out described test paper after, removes solution to be measured unnecessary on test paper；
(3) horizontal positioned test paper makes it develop the color naturally, is compareed with standard color comparison card, obtains the concentration of OPA.
10. detection method according to claim 9, it is characterised in that：The haptoreaction time described in step (1) is 2-
4s；Natural developing time described in step (3) is 1-5min.
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Denomination of invention: A test paper for detection of o-phthalaldehyde and its preparation and detection method Effective date of registration: 20210508 Granted publication date: 20191008 Pledgee: Dezhan health Co.,Ltd. Pledgor: BEIJING CHANGJIANG MAI MEDICAL TECHNOLOGY Co.,Ltd. Registration number: Y2021990000408 |