CN101865911A - Urine creatine quantitative test card - Google Patents
Urine creatine quantitative test card Download PDFInfo
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- CN101865911A CN101865911A CN201010125209A CN201010125209A CN101865911A CN 101865911 A CN101865911 A CN 101865911A CN 201010125209 A CN201010125209 A CN 201010125209A CN 201010125209 A CN201010125209 A CN 201010125209A CN 101865911 A CN101865911 A CN 101865911A
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Abstract
The invention relates to a urine creatine quantitative test card, which comprises an upper shell, a lower shell, reaction test paper and developing test paper arranged in shell cavity, a pipette and a quantitative absorbing rod. The upper shell is provided with a sample loading hole; a solution tank is arranged in the shell; and the tail end of the reaction test paper is positioned in the solution tank. The urine creatine quantitative test card can realize instantaneous measurement of creatine in urine by combining simple and convenient urine quantitative dilution, enzyme reaction and chromatography developing technology, and has the advantages of simple structure, no need of mating instruments, convenient use, low cost, and accordance with application trend of point-of-care testing (POCT).
Description
Technical field
The present invention relates to a kind of urine detection card, especially relate to a kind of urine creatine quantitative test card of measuring creatine concentration in the urine.
Background technology
Creatinine is the product of muscle the human body metabolism, and every 20g muscle metabolism can produce the 1mg creatinine.In muscle, creatine mainly gently forms creatinine by irreversible non-enzyme dehydration, is discharged in the blood, with homaluria again.Creatinine is mainly filtered by glomerulus (important component part of kidney) and excretes.Creatinine is from two kinds of exogenous and endogenouss in the blood, and exogenous creatinine is the meat food product after the metabolism in vivo; The endogenous creatinine is the product of musculature metabolism in the body.When the meat food intake was stablized, the muscle metabolism of health did not have big variation again, and the generation of creatinine will be more constant.
Creatinine is a small-molecule substance, can pass through glomerular filtration, seldom absorbs in renal tubule, and the creatinine that produce in the body every day is almost all discharged with urine, not influenced by the urine amount.During renal insufficiency, creatinine is accumulated in vivo becomes harmful toxin.The UCr inspection can be measured blood and filter the creatinine content of discharging through kidney.Its increase be found in that acromegalia, gigantism, diabetes, infection, thyroid function lower, feed meat, motion, absorption medicine (as vitamin C, levodopa, ethyldopa etc.); UCr lowers and to see acute or chronic renal insufficiency, severe congestive heart failure, hyperthyroidism, anaemia, muscular dystrophy, leukaemia, vegetarian, and takes androgen, thiazide medicine etc.Therefore detect the diagnosis of the variation of UCr content to disease, the variation and the result of treatment of observing disease have important value.The UCr analysis test paper has appearred in recent years, for example application number is the Chinese patent of 200910067221.X, a kind of creatinine urine test paper and preparation method are disclosed, adopt the 2M sodium hydrate buffer solution, pH value 10.5-12.5,1000mL, sodium chloride or potassium chloride 2g-5g, polyvinylpyrrolidone 1g-7g, orange G 100mg-200mg; 3,5-dinitrobenzoic acid 400mg-800mg, methyl alcohol 1000ml.But such test paper can only the half-quantitative detection UCr, can not reach quantitative detection requirement.
Summary of the invention
Technical matters to be solved by this invention provides a kind of urine creatine quantitative test card that adopts enzyme process and chromatography developing to combine to measure creatinine content in the urine.
The present invention solves the technical matters that its technical matters takes: a kind of urine creatine quantitative test card comprises: upper shell, lower house, place reaction test paper and colour developing test paper in the housing cavity, quantitatively suction pipe and quantitative absorbing rod; Upper shell is provided with well, is provided with solution tank in the housing, and described reaction test paper end is positioned at solution tank.
Described reaction test paper includes Creatininase, kreatinase, sarcosine oxidase, horseradish peroxidase, wherein Creatininase content 〉=9U/cm
2, kreatinase content 〉=20U/cm
2, sarcosine oxidase content 〉=3U/cm
2, horseradish peroxidase 〉=3U/cm
2
For convenience of volume calculated, described reaction test paper is x with the total adsorption volume of colour developing test paper, and it is a=x*72/50 that then quantitative suction pipe quantitatively absorbs volume, and it is a=x*3/50 that quantitative absorbing rod quantitatively absorbs volume.
Observe for convenience, the upper shell of described colour developing test paper top is provided with a view window, and the view window subscript is provided with scale mark, and scale marker is arranged.Described upper shell proximal portion is provided with one and finishes indicator hole, contains water-soluble end point indicator on the colour developing test paper of indicator hole below.
For the ease of absorbing moisture and being convenient to operation, described quantitative absorbing rod adopts hydrophilic porous material; Described quantitative absorbing rod one end is inserted in the hydrophobic material, forms handheld terminal; The diameter of described quantitative absorbing rod is less than the bore of quantitative suction pipe, and quantitatively the length of absorbing rod is less than the length of quantitative suction pipe.
Because the technique scheme utilization, the present invention compared with prior art has following advantage:
1, the present invention combines the quantitatively dilution of easy urine, enzyme reaction and chromatography developing technology, therefore can realize the instant mensuration to creatinine in the urine.
2, the present invention is simple in structure, need not use by necessary instrument, and easy to use, cost is low, meets the application trend of POCT (point of care testing, the instant detection).
Description of drawings
Fig. 1 is the structural representation of the embodiment of the invention one;
Fig. 2 is the rear view of the embodiment of the invention one;
Fig. 3 is the side view of the embodiment of the invention one;
Fig. 4 is the front view of the embodiment of the invention one;
Fig. 5 is the cut-open view of the embodiment of the invention one;
Fig. 6 is the structural representation behind the removal loam cake of the embodiment of the invention one;
Fig. 7 is the synoptic diagram of the quantitative absorbing rod of the present invention;
Fig. 8 is the synoptic diagram of the quantitative suction pipe of the present invention;
Fig. 9 is UCr testing result figure of the present invention.
1, upper shell 2, well 3, view window 4, end pilot lamp 5, lower house
6, colour developing test paper 7, reaction test paper 8, solution tank 9, handheld terminal 10, suction hose
11, quantitative absorbing rod 12, quantitative suction pipe
Embodiment
Below in conjunction with drawings and Examples the present invention is further described:
Embodiment one
As shown in Figure 1, a kind of urine creatine quantitative test card comprises: upper shell 1, lower house 5, be placed on reaction test paper 7 and colour developing test paper 6 in the housing cavity, quantitatively suction pipe 12 and quantitative absorbing rod 11; Upper shell 1 is provided with well 2, is provided with solution tank 8 in the housing, and described reaction test paper 7 ends are positioned at solution tank 8; Described reaction test paper 7 includes Creatininase, kreatinase, sarcosine oxidase, horseradish peroxidase.
Described reaction test paper 7 is 250uL with colour developing test paper 6 total adsorption volumes; Used quantitative absorbing rod 11 is 15uL for hydrophilic porous property material glass fiber basis weight absorbs volume, and quantitatively absorbing rod 11 1 ends are inserted in the hydrophobic material, forms handheld terminal 9; The solution that used quantitative suction pipe 12 quantitatively absorbs is the phosphate buffer of 360uLpH6.5; The diameter of used quantitative absorbing rod 11 is less than the bore of quantitative suction pipe 12, and quantitatively the length of absorbing rod 11 is less than the length of quantitative suction pipe 12.The upper shell 1 of described colour developing test paper 6 tops is provided with a view window 3, and view window 3 subscripts are provided with scale mark, and scale marker is arranged; Described upper shell 1 proximal portion is provided with one and finishes indicator hole 4, contain water-soluble end point indicator on the colour developing test paper 6 of indicator hole below, after 6 colour developings of colour developing test paper finish, the surplus solution chromatography is to finishing indicator hole 4 places, finish indicator hole 4 places and the indicator color will occur, show that detection has finished and can carry out the result to read.
The preparation method of described reaction test paper 7 is: according to the form below formulated solution, test paper dipped in gets enzyme solutions, dip in repeatedly get after, 40 ℃ of dryings.
In the technique scheme, the preparation method of colour developing test paper 6 is: adopt hydrogen bromide or CDI activated carrier, treat the preferred N-alkyl benzene of the reagent amine material of Covalent Immobilization, this reagent is with developping solution diffusion, the preferred 2-20mmol/L of concentration after fixing.Color Appearance System also comprises another component: benzothiazolone hydrazone class, the preferred 0.01-1mg/mL of concentration.
The course of work of above-mentioned urine creatine quantitative test card is: use quantitative absorbing rod 11 to be filled urine; Tear the mouth of pipe of quantitative suction pipe 12 off, quantitative absorbing rod 11 is put into quantitative suction pipe 12, be inverted suction pipe and pin the mouth of pipe and mix; Solution in the suction pipe is all added the solution tanks 8 from well 2, reaction test paper 7 by capillary action with the solution chromatography to the test paper 6 that develops the color; Colour developing test paper 6 presents the bluish violet colour band of certain altitude, by the concentration of creatinine in the colour band height indication urine.
The using method of above-mentioned urine creatine quantitative test card is:
1. the horizontal positioned test card uses quantitative absorbing rod 11 to be filled urine;
2. tear the mouth of pipe of quantitative suction pipe 12 off, quantitative absorbing rod 11 is put into quantitative suction pipe 12, be inverted suction pipe and pin the mouth of pipe and mix
3. solution in the suction pipe is all added the solution tank 8 from well 2, start reaction;
4. wait for 10-12 minute, reaction finished when indicator turned green in finishing indicator hole 4;
5. read the colour developing height, finding out corresponding concentration of urinary creatinine in the table of comparisons as a result.
Urine creatinine testing result, as following table and shown in Figure 7:
Claims (8)
1. urine creatine quantitative test card, comprise: upper shell (1), lower house (5), place reaction test paper (7) and colour developing test paper (6) in the housing cavity, quantitatively suction pipe (12) and quantitative absorbing rod (11), it is characterized in that, upper shell (1) is provided with well (2), be provided with solution tank (8) in the housing, described reaction test paper (7) end is positioned at solution tank (8).
2. according to the described urine creatine quantitative test card of claim 1, it is characterized in that described reaction test paper (7) includes Creatininase, kreatinase, sarcosine oxidase, horseradish peroxidase, wherein Creatininase content 〉=9U/cm
2, kreatinase content 〉=20U/cm
2, sarcosine oxidase content 〉=3U/cm
2, horseradish peroxidase 〉=3U/cm
2
3. according to the described urine creatine quantitative test card of claim 1, it is characterized in that, described reaction test paper (7) is x with the total adsorption volume of colour developing test paper (6), and it is a=x*72/50 that then quantitative suction pipe (12) quantitatively absorbs volume, and it is a=x*3/50 that quantitative absorbing rod (11) quantitatively absorbs volume.
4. according to the described urine creatine quantitative test card of claim 1, it is characterized in that the upper shell (1) of described colour developing test paper (6) top is provided with view window (3), view window (3) subscript is provided with scale mark, and scale marker is arranged.
5. according to the described urine creatine quantitative test card of claim 1, it is characterized in that described upper shell (1) proximal portion is provided with one and finishes indicator hole (4), contains water-soluble end point indicator on the colour developing test paper (6) of end indicator hole (4) below.
6. according to the described urine creatine quantitative test card of claim 1, it is characterized in that described quantitative absorbing rod (11) adopts hydrophilic porous material.
7. according to the described urine creatine quantitative test card of claim 1, it is characterized in that described quantitative absorbing rod (11) one ends are inserted in the hydrophobic material, form handheld terminal (9).
8. according to the described urine creatine quantitative test card of claim 1, it is characterized in that the diameter of described quantitative absorbing rod (11) is less than the bore of quantitative suction pipe (12), quantitatively the length of absorbing rod (11) is less than the length of quantitative suction pipe (12).
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|---|---|---|---|
| CN201010125209A CN101865911A (en) | 2010-03-16 | 2010-03-16 | Urine creatine quantitative test card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010125209A CN101865911A (en) | 2010-03-16 | 2010-03-16 | Urine creatine quantitative test card |
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| CN101865911A true CN101865911A (en) | 2010-10-20 |
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| CN201010125209A Pending CN101865911A (en) | 2010-03-16 | 2010-03-16 | Urine creatine quantitative test card |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102375066A (en) * | 2011-09-20 | 2012-03-14 | 天津希恩思生化科技有限公司 | Creatinine content detecting reagent and kit, and manufacturing and using methods of kit |
| CN103278468A (en) * | 2013-05-24 | 2013-09-04 | 宁波美康生物科技股份有限公司 | Creatinine detection reagent |
| CN103575913A (en) * | 2013-11-18 | 2014-02-12 | 无锡博慧斯生物医药科技有限公司 | Urine analysis and test card for urine microalbumin/urine creatinine |
| CN105158452A (en) * | 2015-09-22 | 2015-12-16 | 山东耀华医疗器械股份有限公司 | Urine test paper health test bar integrating preservation and detection |
| CN105334211A (en) * | 2015-10-20 | 2016-02-17 | 北京中生金域诊断技术股份有限公司 | Kit simultaneously detecting sodium and creatinine in urine |
| CN105388146A (en) * | 2015-10-20 | 2016-03-09 | 北京中生金域诊断技术股份有限公司 | Kit for simultaneously detecting sodium, creatinine and microalbumin in urine |
| CN105705202A (en) * | 2013-09-05 | 2016-06-22 | 艾德克斯实验室公司 | Methods for detecting renal disease |
| CN106018397A (en) * | 2016-06-21 | 2016-10-12 | 华南理工大学 | Test paper for detecting activity of alkaline xylanase and preparation method of test paper |
| CN109444054A (en) * | 2018-12-26 | 2019-03-08 | 北京倍肯恒业科技发展股份有限公司 | The detection card and method of piroxicam content in a kind of quick detection dispelling wind and eliminating dampness Chinese medicine |
| CN110554035A (en) * | 2019-08-29 | 2019-12-10 | 南方医科大学南方医院 | Test paper for determining sodium content in urine and use method thereof |
| CN113418916A (en) * | 2021-07-02 | 2021-09-21 | 安徽惠邦生物工程有限公司 | Creatinine quantitative rapid detection test strip and preparation method thereof |
| US11422136B2 (en) | 2017-10-19 | 2022-08-23 | Idexx Laboratories, Inc. | Detection of symmetrical dimethylarginine |
| US11913942B2 (en) | 2015-02-20 | 2024-02-27 | Idexx Laboratories, Inc. | Homogenous immunoassay with compensation for background signal |
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| CN103472041B (en) * | 2011-09-20 | 2016-01-20 | 天津希恩思生化科技有限公司 | A kind of using method of creatinine content detection kit |
| CN103472239B (en) * | 2011-09-20 | 2016-03-09 | 天津希恩思生化科技有限公司 | A kind of preparation method of kreatinin content detecting reagent of three-decker |
| CN102375066B (en) * | 2011-09-20 | 2013-10-16 | 天津希恩思生化科技有限公司 | Creatinine content detecting reagent and kit, and manufacturing and using methods of kit |
| CN103472041A (en) * | 2011-09-20 | 2013-12-25 | 天津希恩思生化科技有限公司 | Use method for creatinine content detection kit |
| CN103472239A (en) * | 2011-09-20 | 2013-12-25 | 天津希恩思生化科技有限公司 | Preparation method of creatinine content detection reagent with three-layer structure |
| CN102375066A (en) * | 2011-09-20 | 2012-03-14 | 天津希恩思生化科技有限公司 | Creatinine content detecting reagent and kit, and manufacturing and using methods of kit |
| CN103278468A (en) * | 2013-05-24 | 2013-09-04 | 宁波美康生物科技股份有限公司 | Creatinine detection reagent |
| CN103278468B (en) * | 2013-05-24 | 2015-09-02 | 宁波美康生物科技股份有限公司 | A kind of creatinine detection reagent |
| CN105705202A (en) * | 2013-09-05 | 2016-06-22 | 艾德克斯实验室公司 | Methods for detecting renal disease |
| US11035861B2 (en) | 2013-09-05 | 2021-06-15 | Idexx Laboratories, Inc. | Methods for detecting renal disease |
| CN103575913B (en) * | 2013-11-18 | 2015-08-19 | 无锡博慧斯生物医药科技有限公司 | The urinalysis test card of microdose urine protein/UCr |
| CN103575913A (en) * | 2013-11-18 | 2014-02-12 | 无锡博慧斯生物医药科技有限公司 | Urine analysis and test card for urine microalbumin/urine creatinine |
| US11913942B2 (en) | 2015-02-20 | 2024-02-27 | Idexx Laboratories, Inc. | Homogenous immunoassay with compensation for background signal |
| CN105158452A (en) * | 2015-09-22 | 2015-12-16 | 山东耀华医疗器械股份有限公司 | Urine test paper health test bar integrating preservation and detection |
| CN105334211A (en) * | 2015-10-20 | 2016-02-17 | 北京中生金域诊断技术股份有限公司 | Kit simultaneously detecting sodium and creatinine in urine |
| CN105334211B (en) * | 2015-10-20 | 2018-02-16 | 北京大学人民医院 | It is a kind of while detect the kit of sodium and creatinine in urine |
| CN105388146A (en) * | 2015-10-20 | 2016-03-09 | 北京中生金域诊断技术股份有限公司 | Kit for simultaneously detecting sodium, creatinine and microalbumin in urine |
| CN106018397B (en) * | 2016-06-21 | 2018-10-09 | 华南理工大学 | A kind of detection alkalescent xylanase vigor test paper and preparation method thereof |
| CN106018397A (en) * | 2016-06-21 | 2016-10-12 | 华南理工大学 | Test paper for detecting activity of alkaline xylanase and preparation method of test paper |
| US11422136B2 (en) | 2017-10-19 | 2022-08-23 | Idexx Laboratories, Inc. | Detection of symmetrical dimethylarginine |
| CN109444054A (en) * | 2018-12-26 | 2019-03-08 | 北京倍肯恒业科技发展股份有限公司 | The detection card and method of piroxicam content in a kind of quick detection dispelling wind and eliminating dampness Chinese medicine |
| CN110554035A (en) * | 2019-08-29 | 2019-12-10 | 南方医科大学南方医院 | Test paper for determining sodium content in urine and use method thereof |
| CN113418916A (en) * | 2021-07-02 | 2021-09-21 | 安徽惠邦生物工程有限公司 | Creatinine quantitative rapid detection test strip and preparation method thereof |
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