CN110554035A - Test paper for determining sodium content in urine and use method thereof - Google Patents
Test paper for determining sodium content in urine and use method thereof Download PDFInfo
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- CN110554035A CN110554035A CN201910809771.8A CN201910809771A CN110554035A CN 110554035 A CN110554035 A CN 110554035A CN 201910809771 A CN201910809771 A CN 201910809771A CN 110554035 A CN110554035 A CN 110554035A
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- 210000002700 urine Anatomy 0.000 title claims abstract description 113
- 238000012360 testing method Methods 0.000 title claims abstract description 71
- 239000011734 sodium Substances 0.000 title claims abstract description 67
- 229910052708 sodium Inorganic materials 0.000 title claims abstract description 62
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 title abstract description 22
- 238000001514 detection method Methods 0.000 claims abstract description 61
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims abstract description 58
- 229910001414 potassium ion Inorganic materials 0.000 claims abstract description 53
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 claims abstract description 40
- 229910001415 sodium ion Inorganic materials 0.000 claims abstract description 34
- 239000000758 substrate Substances 0.000 claims abstract description 29
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 claims abstract description 28
- VCJMYUPGQJHHFU-UHFFFAOYSA-N iron(3+);trinitrate Chemical compound [Fe+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O VCJMYUPGQJHHFU-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000002250 absorbent Substances 0.000 claims abstract description 4
- 230000002745 absorbent Effects 0.000 claims abstract description 4
- 239000000123 paper Substances 0.000 claims description 58
- 239000000243 solution Substances 0.000 claims description 19
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 18
- NGZXDRGWBULKFA-VSGBNLITSA-N curine Chemical compound C([C@H]1N(C)CCC=2C=C(C(=C(OC3=CC=C(C=C3)C[C@@H]3C=4C=C(C(=CC=4CCN3C)OC)O3)C=21)O)OC)C1=CC=C(O)C3=C1 NGZXDRGWBULKFA-VSGBNLITSA-N 0.000 claims description 15
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 239000012086 standard solution Substances 0.000 claims description 12
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 10
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 229910017604 nitric acid Inorganic materials 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000011591 potassium Substances 0.000 claims description 8
- 229910052700 potassium Inorganic materials 0.000 claims description 8
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 claims description 7
- GBZANUMDJPCQHY-UHFFFAOYSA-L mercury(ii) thiocyanate Chemical compound [Hg+2].[S-]C#N.[S-]C#N GBZANUMDJPCQHY-UHFFFAOYSA-L 0.000 claims description 7
- CGOSIVYNVKVZBK-UHFFFAOYSA-K sodium cobalt(2+) trinitrite Chemical compound [Na+].[Co++].[O-]N=O.[O-]N=O.[O-]N=O CGOSIVYNVKVZBK-UHFFFAOYSA-K 0.000 claims description 7
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 238000011161 development Methods 0.000 claims description 6
- 239000001103 potassium chloride Substances 0.000 claims description 6
- 235000011164 potassium chloride Nutrition 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 230000002378 acidificating effect Effects 0.000 claims description 3
- 239000000460 chlorine Substances 0.000 claims description 3
- 229910052801 chlorine Inorganic materials 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 2
- 238000012544 monitoring process Methods 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 235000015424 sodium Nutrition 0.000 description 40
- 150000001450 anions Chemical class 0.000 description 14
- 150000001768 cations Chemical class 0.000 description 8
- 239000003792 electrolyte Substances 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 208000009798 Craniopharyngioma Diseases 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- 238000005048 flame photometry Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 208000020832 chronic kidney disease Diseases 0.000 description 2
- 208000022831 chronic renal failure syndrome Diseases 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 208000009304 Acute Kidney Injury Diseases 0.000 description 1
- 208000003918 Acute Kidney Tubular Necrosis Diseases 0.000 description 1
- 208000016998 Conn syndrome Diseases 0.000 description 1
- 208000014311 Cushing syndrome Diseases 0.000 description 1
- 206010029164 Nephrotic syndrome Diseases 0.000 description 1
- MYEJFUXQJGHEQK-ALRJYLEOSA-N Proscillaridin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@@H]1C=C2CC[C@H]3[C@@]4(O)CC[C@H](C5=COC(=O)C=C5)[C@@]4(C)CC[C@@H]3[C@@]2(C)CC1 MYEJFUXQJGHEQK-ALRJYLEOSA-N 0.000 description 1
- 206010037596 Pyelonephritis Diseases 0.000 description 1
- 208000033626 Renal failure acute Diseases 0.000 description 1
- 206010038540 Renal tubular necrosis Diseases 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 201000011040 acute kidney failure Diseases 0.000 description 1
- 208000012998 acute renal failure Diseases 0.000 description 1
- -1 ammonium ions Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 1
- 238000007917 intracranial administration Methods 0.000 description 1
- SUBFIBLJQMMKBK-UHFFFAOYSA-K iron(3+);trithiocyanate Chemical compound [Fe+3].[S-]C#N.[S-]C#N.[S-]C#N SUBFIBLJQMMKBK-UHFFFAOYSA-K 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 235000014659 low sodium diet Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 208000013846 primary aldosteronism Diseases 0.000 description 1
- 201000009395 primary hyperaldosteronism Diseases 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 235000015598 salt intake Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 208000026775 severe diarrhea Diseases 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to the technical field of urine sodium detection, and discloses test paper for determining sodium content in urine, which comprises a substrate, wherein the substrate comprises a pH detection area, a chloride ion detection area, a potassium ion detection area and a sodium ion colorimetric area, the pH detection area is internally provided with pH test paper and a pH colorimetric card, the chloride ion detection area is internally provided with chloride ion test paper and a chloride ion colorimetric card, the potassium ion detection area is internally provided with potassium ion test paper and a potassium ion colorimetric card, the sodium ion colorimetric card combined with the chloride ion colorimetric card and the potassium ion colorimetric card is arranged in the sodium ion colorimetric area, and the sodium ion content in the urine can be obtained through the sodium ion colorimetric card; ferric nitrate is soaked on the chloride ion detection test paper, and the potassium ion detection test paper is white absorbent paper; the test paper is used for clinically detecting the content of sodium ions in urine of a patient, is suitable for monitoring the condition of the urine sodium of the patient at any time, and has the advantages of simple and convenient operation method and low cost.
Description
Technical Field
The invention relates to the technical field of urine sodium detection, in particular to test paper for determining sodium content in urine and a using method thereof.
background
Urine sodium, i.e. the sodium content in urine, means that the sodium ion concentration in urine is measured for 24 h. The sodium secretion in the night of a human body is about 1/5 of the sodium peak secretion in the day, which shows that the sodium excretion is greatly changed within 1 day, so that the measurement of the sodium in urine needs to take all urine for 24 hours.
Currently, sodium ion detection methods include chemical methods, flame photometry, and ion selective electrode methods. Among them, the chemical method has been gradually replaced by the highly sensitive and fast instrument, and most hospitals now use the flame photometry and the ion selective electrode method, which has fast response, sensitivity and accurate result. In recent years, an electrolyte enzyme method determination method appears, and the method can be used for detecting with a full-automatic analyzer together with other conventional detection items, and is accurate and convenient. However, both the flame photometric method, the ion selective electrode method and the electrolyte enzymatic method require the preparation of a standard detection solution, and the detection of sodium in urine is carried out by matching with a high-precision detection instrument.
However, the clinical needs for some patients to monitor the sodium ion content in urine from time to time, such as: the urinary sodium content is monitored at any time for craniopharyngioma patients, postoperative patients with electrolyte disorder, high-sodium or low-sodium or dehydrated patients, and the like. If a urine sample needs to be taken every ten minutes, the detection is carried out by any one of flame photometry, ion selective electrode method and electrolyte enzyme method, which still has the disadvantages of time and labor consumption and relatively high cost.
Aiming at the phenomenon, the applicant develops the test paper which is convenient to use and low in cost and can be used for measuring the sodium content in urine in time.
disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the test paper for measuring the sodium content in urine, which is used for clinically detecting the sodium ion content in the urine of a patient, is suitable for the condition that the sodium in the urine of the patient needs to be monitored at any time, is simple and convenient to operate and has low cost.
In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows:
The test paper for determining the sodium content in urine comprises a substrate, wherein the substrate comprises a pH detection area, a chloride ion detection area, a potassium ion detection area and a sodium ion colorimetric area, the pH detection area is internally provided with pH test paper and a pH colorimetric card, the chloride ion detection area is internally provided with chloride ion test paper and a chloride ion colorimetric card, the potassium ion detection area is internally provided with potassium ion test paper and a potassium ion colorimetric card, the sodium ion colorimetric card combining the chloride ion colorimetric card and the potassium ion colorimetric card is arranged in the sodium ion colorimetric area, and the sodium ion content in urine can be obtained through the sodium ion colorimetric card; ferric nitrate is soaked on the chloride ion detection test paper, and the potassium ion detection test paper is white absorbent paper.
In the present invention, the substrate includes an upper substrate and a lower substrate, the upper substrate and the lower substrate are both made of hard cardboard, and are integrally formed, and a crease is formed at a boundary.
In the present invention, the upper base plate is further provided with an extending portion, the extending portion is also made of hard cardboard, and a crease is formed at a boundary with the upper base plate.
In the invention, further, a nail button is arranged on the extension part, and a nail button groove matched with the nail button is arranged on the lower substrate.
In the invention, further, the sodium ion colorimetric card comprises a transverse column formed by the chloride ion colorimetric card and a longitudinal column formed by the potassium ion colorimetric card, and chloride ion color blocks of the transverse column are light color blocks to dark color blocks from left to right; the potassium ions of the longitudinal nematic column are from dark color blocks to light color blocks from top to bottom.
In the invention, further, the preparation method of the chloride ion colorimetric card comprises the following steps: preparing 0.01mg/mL sodium chloride standard solution, sequentially sucking 0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL, 12.0mL and 20.0mL of sodium chloride standard solution, respectively placing in a 50mL volumetric flask, adding 5mL of nitric acid, 5mL of ferric nitrate and 20mL of mercury thiocyanate solution, shaking uniformly, adding water to dilute to a scale, standing for 30min for color development, and dividing corresponding color ranges to obtain the chloride ion colorimetric card.
In the invention, further, the preparation method of the potassium ion colorimetric card comprises the following steps: preparing 0.01mg/mL potassium chloride standard solution, sequentially sucking 0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL, 12.0mL and 20.0mL potassium chloride standard solution, respectively placing in a 50mL volumetric flask, 5mL acetic acid, shaking uniformly, dropwise adding 5mL cobalt sodium nitrite, shaking uniformly, adding water to dilute to a scale, standing for color development, and dividing corresponding color ranges to obtain the potassium ion colorimetric card.
In the invention, further, the test paper is converted by the following formula to obtain the sodium ion content range in urine:
CUrine sodium=CUrine chlorine-Curine potassium-a，
Wherein, CUrine sodiumIs the concentration of sodium ions in urine, CUrine chlorineis the concentration of chloride ions in urine, CUrine potassiumIs the concentration of potassium ions in urine, and a is the coefficient of difference.
The anion gap, also called Anion Gap (AG), means the difference between the concentration of an Undetermined Anion (UA) and an Undetermined Cation (UC) in urine, that is, AG = UA-UC., since the total number of equivalents of anions and cations in urine is equal, there are a measured cation (Na +), an Undetermined Cation (UC) = a measured anion (Cl- + HCO 3 -) + an Undetermined Anion (UA), and the anion gap does not change much in the conventional case, and therefore, the sodium content in urine is measured in advance at the time of the measurement, and the average value is taken.
The use method of the test paper for determining the sodium content in urine comprises the following steps:
(1) Detecting chloride ions in urine: placing 0.5mL urine sample in a 10mL Schlenk tube, detecting the pH value of the urine sample by using pH test paper, if the urine sample is alkaline, slowly adding nitric acid while shaking to neutralize the urine until the pH value is 7, cooling to room temperature, adding 1mL nitric acid and 4mL mercury thiocyanate solution, adding water to dilute to a scale, shaking uniformly, standing for 30min, dropwise adding the reaction solution onto chloride ion detection test paper, and comparing by using a chloride ion colorimetric card;
(2) Detecting potassium ions in urine: putting another 0.5mL urine sample in a 10mL Schlenk tube, detecting the pH value of the urine sample by using a pH test paper, if the urine sample is acidic, slowly adding a sodium hydroxide solution while shaking to neutralize the urine to be weakly alkaline, heating the urine sample at 60-80 ℃ for 5-10 minutes, cooling the urine sample, adding 1mL acetic acid after shaking uniformly, dropwise adding 1mL cobalt sodium nitrite, dropwise adding the reaction solution onto a potassium ion detection test paper, and comparing the reaction solution by using a potassium ion colorimetric card;
(3) And (3) obtaining the sodium content in the urine by combining the color block range of the chloride ion detection test paper after comparing with the chloride ion colorimetric card and the color block range of the potassium ion detection test paper after comparing with the potassium ion colorimetric card.
the principle of detecting chloride ions in urine is as follows: and (3) reacting chloride ions in the urine with excessive mercury thiocyanate to generate mercury chloride precipitate, reacting residual redundant thiocyanate radicals with chloride ion detection test paper soaked with ferric nitrate to generate red ferric thiocyanate, and comparing the red ferric thiocyanate radicals with a chloride ion colorimetric card to obtain the large content range of the chloride ions in the urine.
The principle for detecting potassium ions in urine is that ammonium ions in urine are firstly removed to avoid the influence on the sodium cobalt nitrite, according to the chemical reaction formula of 2K + + Na + + [ Co (NO2)6] 3- → K 2 Na [ Co (NO 2) 6 ] ↓,
K 2 Na [ Co (NO 2) 6 ] is yellow precipitate, and the content range of potassium ions in urine is obtained by comparing with potassium ion color comparison card.
in the invention, further, the test paper is mainly applied to the rapid comparison detection of the sodium content in urine.
Due to the adoption of the technical scheme, the invention has the beneficial effects that:
The test paper for determining the sodium content in urine is used for clinically detecting the sodium ion content in the urine of a patient, and is suitable for monitoring the condition of the urine sodium of the patient at any time; the test paper has simple operation method, can complete detection in 5-10 min, does not need to be matched with a precise instrument in the detection process, and reduces the cost.
the test paper of the invention is used for detecting the content of sodium ions in urine of a patient clinically, and has wide application prospect:
1) The instant sodium urine determination can be used to determine whether salt intake is sufficient and whether there is a large loss of salt, and can also assist in monitoring low-salt diets and in determining electrolyte balance in patients with vomiting and severe diarrhea;
2) The kidney is a main excretory organ of sodium salt, the function and pathological change condition of the kidney can be known by measuring natrium urinae, and the immediate natrium urinae detection can assist patients and families to quickly measure natrium urinae for patients with serious pyelonephritis, acute tubular necrosis, nephrotic syndrome, acute or chronic renal failure, cushing syndrome, primary aldosteronism, chronic renal failure late stage and the like, thereby providing reliable basis for the clinician to timely and effectively treat the disease;
3) The tumor in the saddle area is a common intracranial tumor, the perioperative treatment is an important factor for determining the prognosis of a patient, and the treatment of electrolyte disorder is a key link of the perioperative treatment. Therefore, monitoring the instant natrium provides important guarantee for the patients with the tumor in the saddle area represented by the craniopharyngioma to smoothly transit through the perioperative period and improve the long-term survival quality. Therefore, the invention can greatly reduce the complications of patients, shorten the hospitalization time, reduce the medical cost, improve the life quality of the patients, reduce the burden of society and families and have extremely high social and economic benefits.
Drawings
FIG. 1 is a schematic structural diagram of a test strip for determining sodium content in urine.
FIG. 2 is a schematic diagram of the structure of the test strip for determining sodium content in urine in the open state.
In the drawings: 1-substrate, 11-upper substrate, 111-extension part, 112-button, 12-lower substrate, 121-button slot, 21-pH test paper, 22-pH colorimetric card, 31-chloride ion detection test paper, 32-chloride ion colorimetric card, 41-potassium ion detection test paper, 42-potassium ion colorimetric card and 5-sodium ion colorimetric card.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
referring to fig. 1 to 2, a test strip for determining sodium content in urine according to a preferred embodiment of the present invention includes a substrate 1, where the substrate 1 includes a pH detection region, a chloride detection region, a potassium detection region and a sodium colorimetric region, the pH detection region is provided with a pH test strip 21 and a pH colorimetric card 22, the chloride detection region is provided with a chloride test strip 31 and a chloride colorimetric card 32, the potassium detection region is provided with a potassium detection test strip 41 and a potassium colorimetric card 42, the sodium colorimetric region is provided with a sodium colorimetric card 5 combining the chloride colorimetric card 32 and the potassium colorimetric card 42, and the sodium content in urine can be obtained through the sodium colorimetric card 5; the chloride ion detection test paper 31 is soaked with ferric nitrate, and the potassium ion detection test paper 41 is white absorbent paper.
In this embodiment, the substrate 1 includes an upper substrate 11 and a lower substrate 12, the upper substrate 11 and the lower substrate 12 are made of hard cardboard, and are integrally formed with each other and have a fold line at a boundary.
In the present embodiment, the upper base plate 11 is provided with an extension portion 111, and the extension portion 111 is also made of a hard cardboard and has a fold line at a boundary with the upper base plate 11.
In this embodiment, the extension portion 111 is provided with a nail button 112, and the lower substrate 12 is provided with a nail button groove 121 engaged with the nail button 112.
In the present embodiment, the sodium ion colorimetric card 5 includes a horizontal column formed by the chloride ion colorimetric card 32 and a vertical column formed by the potassium ion colorimetric card 42, and chloride ion color patches of the horizontal column are light color patches to dark color patches from left to right; the potassium ions of the longitudinal nematic column are from dark color blocks to light color blocks from top to bottom.
Preparing a chloride ion colorimetric card 32: preparing 0.01mg/mL sodium chloride standard solution, sequentially sucking 0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL, 12.0mL and 20.0mL of sodium chloride standard solution, respectively placing in a 50mL volumetric flask, adding 5mL of nitric acid, 5mL of ferric nitrate and 20mL of mercury thiocyanate solution, shaking uniformly, adding water to dilute to a scale, standing for 30min for color development, and dividing corresponding color ranges to obtain the chloride ion colorimetric card 32.
preparation of potassium ion colorimetric card 42: preparing 0.01mg/mL potassium chloride standard solution, sequentially sucking 0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL, 12.0mL and 20.0mL potassium chloride standard solution, respectively placing in a 50mL volumetric flask, 5mL acetic acid, shaking uniformly, dropwise adding 5mL cobalt sodium nitrite, shaking uniformly, adding water to dilute to a scale, standing for color development, and dividing corresponding color ranges to obtain the potassium ion colorimetric card 42.
The use method of the test paper for determining the sodium content in urine comprises the following steps:
(1) Detecting chloride ions in urine: putting 0.5mL urine sample into a 10mL Schlenk tube, detecting the pH value of the urine sample by using pH test paper, if the urine sample is alkaline, slowly adding nitric acid while shaking to neutralize the urine until the pH value is 7, cooling to room temperature, adding 1mL nitric acid and 4mL mercury thiocyanate solution, adding water to dilute to a scale, shaking uniformly, standing for 30min, dropwise adding the reaction solution onto chloride ion detection test paper 31, and comparing by using a chloride ion colorimetric card 32;
(2) Detecting potassium ions in urine: another 0.5mL urine sample is placed in a 10mL Schlenk tube, the pH value of the urine sample is detected by using a pH test paper, if the urine sample is acidic, sodium hydroxide solution is slowly added while shaking to neutralize the urine to be weakly alkaline, the urine sample is heated at 60-80 ℃ for 5-10 minutes, after cooling, 1mL acetic acid is added, after shaking uniformly, 1mL cobalt sodium nitrite is added dropwise, the reaction solution is added dropwise onto potassium ion detection test paper 41, and comparison is carried out by using a potassium ion colorimetric card 42;
(3) Through the color lump scope behind the chloride ion test paper contrast chloride ion color comparison card, combine the color lump scope behind the potassium ion test paper contrast potassium ion color comparison card, adopt the following formula conversion to obtain the sodium ion content scope in the urine to cut apart into a plurality of urine sodium concentration regions with sodium ion color comparison card 5, conveniently go to read:
CUrine sodium=CUrine chlorine-CUrine potassium-a，
Wherein, CUrine sodiumIs the concentration of sodium ions in urine, CUrine chlorineIs the concentration of chloride ions in urine, CUrine potassiumIs the concentration of potassium ions in urine, and a is the coefficient of difference.
The anion gap, also called Anion Gap (AG), means the difference between the concentration of an Undetermined Anion (UA) and an Undetermined Cation (UC) in urine, that is, AG = UA-UC., since the total number of equivalents of anions and cations in urine is equal, there are a measured cation (Na +), an Undetermined Cation (UC) = a measured anion (Cl- + HCO 3 -) + an Undetermined Anion (UA), and the anion gap does not change much in the conventional case, and therefore, the sodium content in urine is measured in advance at the time of the measurement, and the average value is taken.
The test paper for determining the sodium content in urine is used for clinically detecting the sodium ion content in the urine of a patient, is suitable for the condition that the sodium in the urine of the patient needs to be monitored at any time, namely the condition that the sodium in the urine of the patient with craniopharyngioma, the patient with electrolyte disorder after operation, the patient with high sodium or low sodium or the patient with dehydration needs to be monitored at any time, and has the advantages of simple and convenient operation method and low cost.
The invention is not limited to the foregoing embodiments. The invention extends to any novel feature or any novel combination of features disclosed in this specification and any novel method or process steps or any novel combination of features disclosed. Unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may include the first and second features being in direct contact, or may include the first and second features not being in direct contact but being in contact with each other through another feature therebetween. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Claims (10)
1. the test paper for determining the sodium content in urine is characterized by comprising a substrate (1), wherein the substrate (1) comprises a pH detection area, a chloride ion detection area, a potassium ion detection area and a sodium ion colorimetric area, the pH detection area is internally provided with pH test paper (21) and a pH colorimetric card (22), the chloride ion detection area is internally provided with chloride ion detection test paper (31) and a chloride ion colorimetric card (32), the potassium ion detection area is internally provided with potassium ion detection test paper (41) and a potassium ion colorimetric card (42), the sodium ion colorimetric card (5) combining the chloride ion colorimetric card (32) and the potassium ion colorimetric card (42) is arranged in the sodium ion colorimetric area, and the sodium ion content in urine can be obtained through the sodium ion colorimetric card (5); ferric nitrate is soaked on the chloride ion detection test paper (31), and the potassium ion detection test paper (41) is white absorbent paper.
2. The test paper for determining the sodium content in urine according to claim 1, wherein the substrate (1) comprises an upper substrate (11) and a lower substrate (12), the upper substrate (11) and the lower substrate (12) are made of rigid cardboard, and are integrally formed, and a crease is formed at the boundary.
3. the test paper for determining the sodium content in urine according to claim 2, wherein the upper base plate (11) is provided with an extension part (111), the extension part (111) is also made of hard cardboard, and a crease is formed at the boundary of the extension part and the upper base plate (11).
4. The test paper for determining the content of sodium in urine according to claim 3, wherein the extension portion (111) is provided with a button (112), and the lower substrate (12) is provided with a button slot (121) matched with the button (112).
5. The test paper for determining the sodium content in urine according to claim 1, wherein the sodium ion colorimetric card (5) comprises a horizontal column formed by the chloride ion colorimetric card (32) and a vertical column formed by the potassium ion colorimetric card (42), and chloride ion color patches of the horizontal column are light color patches to dark color patches from left to right; the potassium ions of the longitudinal nematic column are from dark color blocks to light color blocks from top to bottom.
6. The test paper for determining the sodium content in urine according to claim 1, which is prepared by the following steps: preparing 0.01mg/mL sodium chloride standard solution, sequentially sucking 0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL, 12.0mL and 20.0mL of sodium chloride standard solution, respectively placing in a 50mL volumetric flask, adding 5mL of nitric acid, 5mL of ferric nitrate and 20mL of mercury thiocyanate solution, shaking uniformly, adding water to dilute to a scale, standing for 30min for color development, and dividing corresponding color ranges to obtain the chloride ion colorimetric card.
7. The test paper for determining the sodium content in urine according to claim 1, which is prepared by the following steps: preparing 0.01mg/mL potassium chloride standard solution, sequentially sucking 0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL, 12.0mL and 20.0mL potassium chloride standard solution, respectively placing in a 50mL volumetric flask, 5mL acetic acid, shaking uniformly, dropwise adding 5mL cobalt sodium nitrite, shaking uniformly, adding water to dilute to a scale, standing for color development, and dividing corresponding color ranges to obtain the potassium ion colorimetric card.
8. the test strip for determining the sodium content in urine according to claim 1, wherein the test strip is converted into the range of the sodium ion content in urine by the following formula:
CUrine sodium=CUrine chlorine-CUrine potassium-a，
Wherein, CUrine sodiumIs the concentration of sodium ions in urine, CUrine chlorineIs the concentration of chloride ions in urine, CUrine potassiumIs the concentration of potassium ions in urine, and a is the coefficient of difference.
9. Use of a dipstick according to any of claims 1-8 for determining the sodium content of urine, characterised in that it comprises the following steps:
(1) detecting chloride ions in urine: placing 0.5mL urine sample in a 10mL Schlenk tube, detecting the pH value of the urine sample by using pH test paper, if the urine sample is alkaline, slowly adding nitric acid while shaking to neutralize the urine until the pH value is 7, cooling to room temperature, adding 1mL nitric acid and 4mL mercury thiocyanate solution, adding water to dilute to a scale, shaking uniformly, standing for 30min, dropwise adding the reaction solution onto chloride ion detection test paper, and comparing by using a chloride ion colorimetric card;
(2) detecting potassium ions in urine: putting another 0.5mL urine sample in a 10mL Schlenk tube, detecting the pH value of the urine sample by using a pH test paper, if the urine sample is acidic, slowly adding a sodium hydroxide solution while shaking to neutralize the urine to be weakly alkaline, heating the urine sample at 60-80 ℃ for 5-10 minutes, cooling the urine sample, adding 1mL acetic acid after shaking uniformly, dropwise adding 1mL cobalt sodium nitrite, dropwise adding the reaction solution onto a potassium ion detection test paper, and comparing the reaction solution by using a potassium ion colorimetric card;
(3) and (3) obtaining the sodium content in the urine by combining the color block range of the chloride ion detection test paper after comparing with the chloride ion colorimetric card and the color block range of the potassium ion detection test paper after comparing with the potassium ion colorimetric card.
10. The test paper for determining the sodium content in urine according to any one of claims 1 to 8, which is mainly used for rapid comparison detection of the sodium content in urine.
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