CN110554035B - Test paper for measuring sodium content in urine and use method thereof - Google Patents
Test paper for measuring sodium content in urine and use method thereof Download PDFInfo
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- CN110554035B CN110554035B CN201910809771.8A CN201910809771A CN110554035B CN 110554035 B CN110554035 B CN 110554035B CN 201910809771 A CN201910809771 A CN 201910809771A CN 110554035 B CN110554035 B CN 110554035B
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- 210000002700 urine Anatomy 0.000 title claims abstract description 115
- 238000012360 testing method Methods 0.000 title claims abstract description 71
- 239000011734 sodium Substances 0.000 title claims abstract description 59
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 title claims abstract description 57
- 229910052708 sodium Inorganic materials 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 title claims abstract description 34
- 238000001514 detection method Methods 0.000 claims abstract description 63
- 229910001414 potassium ion Inorganic materials 0.000 claims abstract description 59
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims abstract description 55
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 claims abstract description 46
- 229910001415 sodium ion Inorganic materials 0.000 claims abstract description 39
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000000758 substrate Substances 0.000 claims abstract description 33
- VCJMYUPGQJHHFU-UHFFFAOYSA-N iron(3+);trinitrate Chemical compound [Fe+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O VCJMYUPGQJHHFU-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000002250 absorbent Substances 0.000 claims abstract description 4
- 230000002745 absorbent Effects 0.000 claims abstract description 4
- 239000000123 paper Substances 0.000 claims description 65
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 18
- 150000001450 anions Chemical class 0.000 claims description 17
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 12
- 239000012086 standard solution Substances 0.000 claims description 12
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 229910017604 nitric acid Inorganic materials 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- CGOSIVYNVKVZBK-UHFFFAOYSA-K sodium cobalt(2+) trinitrite Chemical compound [Na+].[Co++].[O-]N=O.[O-]N=O.[O-]N=O CGOSIVYNVKVZBK-UHFFFAOYSA-K 0.000 claims description 7
- 230000002485 urinary effect Effects 0.000 claims description 7
- LJBWEZVYRBKOCI-UHFFFAOYSA-N 2,4,6-triaminoquinazoline Chemical compound N1=C(N)N=C(N)C2=CC(N)=CC=C21 LJBWEZVYRBKOCI-UHFFFAOYSA-N 0.000 claims description 6
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 6
- 239000012295 chemical reaction liquid Substances 0.000 claims description 6
- 239000000460 chlorine Substances 0.000 claims description 6
- 229910052801 chlorine Inorganic materials 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 238000011161 development Methods 0.000 claims description 6
- 239000011591 potassium Substances 0.000 claims description 6
- 229910052700 potassium Inorganic materials 0.000 claims description 6
- 239000001103 potassium chloride Substances 0.000 claims description 6
- 235000011164 potassium chloride Nutrition 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 230000002378 acidificating effect Effects 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
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- 238000012544 monitoring process Methods 0.000 abstract description 7
- 235000015424 sodium Nutrition 0.000 description 43
- 150000001768 cations Chemical class 0.000 description 8
- 206010028980 Neoplasm Diseases 0.000 description 6
- 239000003792 electrolyte Substances 0.000 description 5
- 238000005048 flame photometry Methods 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
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- 102000004190 Enzymes Human genes 0.000 description 3
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- 239000011111 cardboard Substances 0.000 description 3
- 206010014418 Electrolyte imbalance Diseases 0.000 description 2
- -1 ammonium ions Chemical class 0.000 description 2
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- 208000022831 chronic renal failure syndrome Diseases 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000029142 excretion Effects 0.000 description 2
- SUBFIBLJQMMKBK-UHFFFAOYSA-K iron(3+);trithiocyanate Chemical compound [Fe+3].[S-]C#N.[S-]C#N.[S-]C#N SUBFIBLJQMMKBK-UHFFFAOYSA-K 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
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- 208000009304 Acute Kidney Injury Diseases 0.000 description 1
- 208000003918 Acute Kidney Tubular Necrosis Diseases 0.000 description 1
- 208000016998 Conn syndrome Diseases 0.000 description 1
- 208000014311 Cushing syndrome Diseases 0.000 description 1
- 206010029164 Nephrotic syndrome Diseases 0.000 description 1
- 206010037596 Pyelonephritis Diseases 0.000 description 1
- 208000033626 Renal failure acute Diseases 0.000 description 1
- 206010038540 Renal tubular necrosis Diseases 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 201000011040 acute kidney failure Diseases 0.000 description 1
- 208000012998 acute renal failure Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 1
- 238000007917 intracranial administration Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 235000014659 low sodium diet Nutrition 0.000 description 1
- 229960002523 mercuric chloride Drugs 0.000 description 1
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 1
- GBZANUMDJPCQHY-UHFFFAOYSA-L mercury(ii) thiocyanate Chemical compound [Hg+2].[S-]C#N.[S-]C#N GBZANUMDJPCQHY-UHFFFAOYSA-L 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 208000013846 primary aldosteronism Diseases 0.000 description 1
- 201000009395 primary hyperaldosteronism Diseases 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 235000015598 salt intake Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 208000026775 severe diarrhea Diseases 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- AWLUSOLTCFEHNE-UHFFFAOYSA-N sodium;urea Chemical compound [Na].NC(N)=O AWLUSOLTCFEHNE-UHFFFAOYSA-N 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to the technical field of sodium urine detection, and discloses test paper for measuring sodium content in urine, which comprises a substrate, wherein the substrate comprises a pH detection area, a chloride ion detection area, a potassium ion detection area and a sodium ion color comparison area, the pH detection area is internally provided with a pH test paper and a pH color comparison card, the chloride ion detection area is internally provided with a chloride ion detection test paper and a chloride ion color comparison card, the potassium ion detection area is internally provided with a potassium ion detection test paper and a potassium ion color comparison card, and the sodium ion color comparison area is internally provided with a sodium ion color comparison card combined with the chloride ion color comparison card and the potassium ion color comparison card, so that the sodium ion content in urine can be obtained through the sodium ion color comparison card; ferric nitrate is immersed on the chloride ion detection test paper, and the potassium ion detection test paper is white absorbent paper; the test paper is used for clinically detecting the sodium ion content in urine of a patient, is suitable for monitoring the sodium in urine of the patient in real time, and has the advantages of simple operation method and low cost.
Description
Technical Field
The invention relates to the technical field of sodium urine detection, in particular to test paper for measuring sodium content in urine and a using method thereof.
Background
Sodium urine, i.e. the sodium content of urine, refers to the determination of the sodium ion concentration in 24h urine. The sodium secretion amount of the human body at night is about 1/5 of the sodium peak secretion amount in the daytime, which shows that the sodium excretion is greatly changed within 1 day, so that the measurement of the sodium in urine needs to leave all urine for 24 hours.
Currently, sodium ion detection methods include a chemical method, a flame photometry method and an ion selective electrode method. Among them, chemical methods have been gradually replaced by highly sensitive, rapid instruments, and most hospitals now use flame photometry and ion selective electrode methods, which are fast in response, sensitive and accurate in results. In recent years, an electrolyte enzyme method determination method has appeared, and the method can be used for detection together with other conventional detection projects by using a full-automatic analyzer, and is accurate and convenient. However, in both the flame photometry and the ion selective electrode method and in the electrolyte enzyme method measurement method, standard detection liquid needs to be prepared, and sodium urine detection is carried out by matching with a high-precision detection instrument.
However, for cases where clinical needs to monitor the sodium ion content of urine from time to time for some patients, for example: the urinary sodium content is required to be monitored from time to time for patients with craniopharyngeal tube tumor, patients with electrolyte disturbance after operation, patients with high sodium or low sodium or dehydration, etc. If urine samples need to be taken every ten minutes, detection by any one of flame photometry, ion selective electrode method and electrolyte enzyme method measurement method is still time-consuming and labor-consuming, and the cost is relatively high.
Aiming at the phenomena, the inventor develops a test paper which is convenient to use and low in cost and can timely measure the sodium content in urine.
Disclosure of Invention
Aiming at the defects existing in the prior art, the invention aims to provide the test paper for measuring the sodium content in urine, which is used for clinically detecting the sodium ion content in urine of a patient, is suitable for monitoring the sodium content of urine of the patient in real time, and has the advantages of simple operation method and low cost.
In order to achieve the above object, the present invention adopts the following technical scheme:
The test paper for measuring the sodium content in urine comprises a substrate, wherein the substrate comprises a pH detection area, a chloride ion detection area, a potassium ion detection area and a sodium ion color comparison area, the pH detection area is internally provided with a pH test paper and a pH color comparison card, the chloride ion detection area is internally provided with a chloride ion detection test paper and a chloride ion color comparison card, the potassium ion detection area is internally provided with a potassium ion detection test paper and a potassium ion color comparison card, and the sodium ion color comparison area is internally provided with a sodium ion color comparison card combined with the chloride ion color comparison card and the potassium ion color comparison card, so that the sodium ion content in urine can be obtained through the sodium ion color comparison card; ferric nitrate is soaked on the chloride ion detection test paper, and the potassium ion detection test paper is white absorbent paper.
In the present invention, the substrate further comprises an upper substrate and a lower substrate, the upper substrate and the lower substrate are made of hard paper boards, and are integrally formed, and the boundary is provided with a crease.
In the present invention, further, an extension portion is provided on the upper substrate, and the extension portion is made of cardboard, and a crease is formed at a boundary between the extension portion and the upper substrate.
In the invention, further, a button sewing groove matched with the button sewing is formed on the lower substrate.
In the invention, further, the sodium ion colorimetric card comprises a transverse column formed by the chloride ion colorimetric card and a longitudinal column formed by the potassium ion colorimetric card, wherein the chloride ion color blocks of the transverse column are light-color blocks to dark-color blocks from left to right; the potassium ions in the longitudinal columns are dark color blocks to light color blocks from top to bottom.
In the invention, further, the preparation method of the chloride ion colorimetric card comprises the following steps: preparing 0.01mg/mL sodium chloride standard solution, sequentially sucking 0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL, 12.0mL and 20.0mL of sodium chloride standard solution, respectively placing into a 50mL volumetric flask, adding 5mL of nitric acid, 5mL of ferric nitrate and 20mL of mercuric thiocyanate solution, shaking uniformly, adding water for dilution to scale, standing for 30min for color development, and dividing corresponding color ranges to obtain the chloride ion color card.
In the invention, further, the preparation method of the potassium ion colorimetric card comprises the following steps: preparing 0.01mg/mL potassium chloride standard solution, sequentially sucking 0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL, 12.0mL and 20.0mL of potassium chloride standard solution, respectively placing the solution in a 50mL volumetric flask, shaking up 5mL of acetic acid, dropwise adding 5mL of sodium cobalt nitrite, shaking up, adding water to dilute to scale, standing for color development, and dividing corresponding color ranges to obtain the potassium ion color card.
In the invention, the test paper is further converted into the content range of sodium ions in urine by adopting the following formula:
C Urine sodium =C Urine chlorine -C Urinary potassium -a，
Wherein, C Urine sodium is the concentration of sodium ions in urine, C Urine chlorine is the concentration of chloride ions in urine, C Urinary potassium is the concentration of potassium ions in urine, and a is the difference coefficient.
The coefficient a is the anion gap. Anion gap, also called Anion Gap (AG): refers to the difference between the concentration of Unmeasured Anions (UA) and Unmeasured Cations (UC) in urine, i.e. ag=ua-UC. Since the total equivalent number of anions and cations in urine is equal, the urine comprises: measured cation (na+) + Unmeasured Cation (UC) =measured anion (Cl- +hco 3 -) + Unmeasured Anion (UA); the anion gap is not changed much in the conventional case, so that the sodium content in urine is measured in advance and averaged.
The application method of the test paper for measuring the sodium content in urine comprises the following steps:
(1) Detecting chloride ions in urine: placing 0.5mL of urine sample into a 10mL Schlenk tube, detecting the pH value by using pH test paper, slowly adding nitric acid to neutralize urine while shaking until the pH value is 7 if the urine sample is alkaline, cooling to room temperature, adding 1mL nitric acid and 4mL mercuric thiocyanate solution, adding water to dilute to a scale, shaking uniformly, standing for 30min, dripping the reaction liquid onto the chloridion detection test paper, and comparing by using a chloridion colorimetric card;
(2) Detecting potassium ions in urine: placing 0.5mL of urine sample in a 10mL Schlenk tube, detecting the pH value by using pH test paper, slowly adding sodium hydroxide solution to neutralize urine to alkalescence while shaking if the urine sample is slightly acidic, heating at 60-80 ℃ for 5-10 minutes, cooling, uniformly shaking 1mL of acetic acid, dropwise adding 1mL of sodium cobalt nitrite, dripping the reaction liquid on potassium ion detection test paper, and comparing by using a potassium ion colorimetric card;
(3) And comparing the color lump range after the chlorine ion color chart with the chlorine ion detection test paper, and combining the color lump range after the potassium ion color chart is compared with the potassium ion detection test paper to obtain the sodium content in the urine.
The principle of detecting chloride ions in urine is as follows: and (3) reacting chloride ions in the urine with excessive mercury thiocyanate to generate mercuric chloride precipitate, wherein excessive thiocyanate radicals are remained in the reaction and react with ferric nitrate immersed on the chloride ion detection test paper to generate haematochrome ferric thiocyanate, and comparing the haematochrome ferric thiocyanate with the chloride ion colorimetric card to obtain the general content range of the chloride ions in the urine.
The principle of detecting potassium ions in urine is as follows: ammonium ions in urine are removed firstly, so that the influence of the ammonium ions on sodium cobalt nitrite is avoided; according to the chemical reaction formula: 2K ++Na++[Co(NO2)6]3-→K2Na[Co(NO2)6 ∈,
K 2Na[Co(NO2)6 is yellow precipitate, and the general content range of potassium ions in urine is obtained through colorimetric comparison with a potassium ion colorimetric card.
In the invention, further, the test paper is mainly applied to rapid comparison detection of sodium content in urine.
Due to the adoption of the technical scheme, the invention has the beneficial effects that:
the test paper for measuring sodium content in urine is used for clinically detecting sodium ion content in urine of a patient, and is suitable for monitoring sodium in urine of the patient in real time; the test paper has the advantages of simple operation method, detection completion within 5-10 minutes at maximum, no need of matching with precise instruments in the detection process, and reduced cost.
The test paper is used for clinically detecting the sodium ion content in urine of a patient, and has wide application prospect:
1) The instant sodium urine measurement can be used for determining whether the salt intake is enough or whether a great amount of salt is lost, and can also assist in monitoring low-salt diet and helping to judge electrolyte balance of patients suffering from vomiting and severe diarrhea;
2) The kidney is a main excretion organ of sodium salt, the function and pathological condition of the kidney can be known by measuring sodium urine, and reliable basis is provided for a clinician to timely and effectively treat serious pyelonephritis, acute tubular necrosis, nephrotic syndrome, acute or chronic renal failure, cushing's syndrome, primary aldosteronism, advanced stage of chronic renal failure and the like, and the patient and family members can be assisted in rapidly measuring sodium urine by instant sodium urine detection;
3) Saddle area tumors are common intracranial tumors, perioperative treatment is an important factor for determining prognosis of patients, and electrolyte disorder treatment is a key link of perioperative treatment. Therefore, monitoring the instant sodium urea provides important guarantee for patients with saddle area tumor represented by craniopharyngeal tumor to stably pass through the perioperative period and improve the long-term life quality. Therefore, the invention can greatly reduce the complications of patients, shorten the hospitalization time, reduce the medical cost, improve the life quality of the patients, lighten the burden of society and families and has extremely high social and economic benefits.
Drawings
FIG. 1 is a schematic diagram of the structure of a test paper for determining sodium content in urine.
Fig. 2 is a schematic diagram of the structure of the test paper for measuring sodium content in urine in an open state.
In the accompanying drawings: 1-base plate, 11-upper base plate, 111-extension, 112-button sewing, 12-lower base plate, 121-button sewing groove, 21-pH test paper, 22-pH color chart, 31-chloridion test paper, 32-chloridion color chart, 41-potassium ion test paper, 42-potassium ion color chart and 5-sodium ion color chart.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1 to 2, a preferred embodiment of the present invention provides a test paper for determining sodium content in urine, which comprises a substrate 1, wherein the substrate 1 comprises a pH detection area, a chloride ion detection area, a potassium ion detection area and a sodium ion color comparison area, the pH detection area is internally provided with a pH test paper 21 and a pH color comparison card 22, the chloride ion detection area is internally provided with a chloride ion detection test paper 31 and a chloride ion color comparison card 32, the potassium ion detection area is internally provided with a potassium ion detection test paper 41 and a potassium ion color comparison card 42, and the sodium ion color comparison area is internally provided with a sodium ion color comparison card 5 for combining the chloride ion color comparison card 32 and the potassium ion color comparison card 42, so that the sodium content in urine can be obtained through the sodium ion color comparison card 5; the chloride ion test paper 31 is impregnated with ferric nitrate, and the potassium ion test paper 41 is white absorbent paper.
In this embodiment, the substrate 1 includes an upper substrate 11 and a lower substrate 12, the upper substrate 11 and the lower substrate 12 are made of cardboard, and are integrally formed, and a crease is formed at a boundary.
In the present embodiment, the upper substrate 11 is provided with an extension 111, and the extension 111 is made of cardboard, and a crease is formed at a boundary between the upper substrate 11 and the extension 111.
In this embodiment, the extension 111 is provided with a button 112, and the lower substrate 12 is provided with a button slot 121 that mates with the button 112.
In the present embodiment, the sodium ion color chart 5 includes a horizontal row formed by the chloride ion color chart 32 and a vertical row formed by the potassium ion color chart 42, and the chloride ion color patches of the horizontal row are light color patches to dark color patches from left to right; the potassium ions in the longitudinal columns are dark color blocks to light color blocks from top to bottom.
Preparation of chloride ion color chart 32: preparing 0.01mg/mL sodium chloride standard solution, sequentially sucking 0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL, 12.0mL and 20.0mL of sodium chloride standard solution, respectively placing into a 50mL volumetric flask, adding 5mL of nitric acid, 5mL of ferric nitrate and 20mL of mercuric thiocyanate solution, shaking uniformly, adding water for dilution to scale, standing for 30min for color development, and dividing corresponding color ranges to obtain the chloride ion color card 32.
Preparation of potassium ion color chart 42: preparing 0.01mg/mL potassium chloride standard solution, sequentially sucking 0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL, 12.0mL and 20.0mL of potassium chloride standard solution, respectively placing the solution in a 50mL volumetric flask, shaking up 5mL of acetic acid, dropwise adding 5mL of sodium cobalt nitrite, shaking up, adding water to dilute to scale, standing for color development, and dividing corresponding color ranges to obtain the potassium ion color card 42.
The application method of the test paper for measuring the sodium content in urine comprises the following steps:
(1) Detecting chloride ions in urine: placing 0.5mL of urine sample into a 10mL Schlenk tube, detecting the pH value by using pH test paper, slowly adding nitric acid to neutralize urine while shaking until the pH value is 7 if the urine sample is alkaline, cooling to room temperature, adding 1mL nitric acid and 4mL mercuric thiocyanate solution, adding water to dilute to a scale, shaking uniformly, standing for 30min, dripping the reaction liquid onto chloride ion detection test paper 31, and comparing by using a chloride ion colorimetric card 32;
(2) Detecting potassium ions in urine: placing 0.5mL of urine sample in a 10mL Schlenk tube, detecting the pH value by using pH test paper, slowly adding sodium hydroxide solution to neutralize urine to alkalescence while shaking if the urine sample is slightly acidic, heating at 60-80 ℃ for 5-10 minutes, cooling, uniformly shaking 1mL of acetic acid, dropwise adding 1mL of sodium cobalt nitrite, dripping the reaction liquid on potassium ion detection test paper 41, and comparing by using a potassium ion colorimetric card 42;
(3) The color lump range after the chlorine ion test paper is compared with the chlorine ion color chart is combined with the color lump range after the potassium ion color chart is compared with the potassium ion test paper, the sodium ion content range in urine is obtained by adopting the following formula, and the sodium ion color chart 5 is divided into a plurality of urine sodium concentration areas, so that the reading is convenient:
C Urine sodium =C Urine chlorine -C Urinary potassium -a，
Wherein, C Urine sodium is the concentration of sodium ions in urine, C Urine chlorine is the concentration of chloride ions in urine, C Urinary potassium is the concentration of potassium ions in urine, and a is the difference coefficient.
The coefficient a is the anion gap. Anion gap, also called Anion Gap (AG): refers to the difference between the concentration of Unmeasured Anions (UA) and Unmeasured Cations (UC) in urine, i.e. ag=ua-UC. Since the total equivalent number of anions and cations in urine is equal, the urine comprises: measured cation (na+) + Unmeasured Cation (UC) =measured anion (Cl- +hco 3 -) + Unmeasured Anion (UA); the anion gap is not changed much in the conventional case, so that the sodium content in urine is measured in advance and averaged.
The test paper for measuring the sodium content in the urine is used for clinically detecting the sodium ion content in the urine of a patient, is suitable for monitoring the sodium in the urine of the patient in real time, namely, monitoring the sodium in the urine of a craniopharyngeal tube tumor patient, a patient with electrolyte disturbance stage after operation, a patient with high sodium or low sodium or a patient with dehydration in real time, and has the advantages of simple operation method and low cost.
The invention is not limited to the specific embodiments described above. The invention extends to any novel one, or any novel combination, of the features disclosed in this specification, as well as to any novel one, or any novel combination, of the steps of the method or process disclosed. Unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both first and second features being in direct contact, and may also include both first and second features not being in direct contact but being in contact with each other by way of additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.
The foregoing description is directed to the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the invention, and all equivalent changes or modifications made under the technical spirit of the present invention should be construed to fall within the scope of the present invention.
Claims (7)
1. The use method of the test paper for determining the sodium content in urine is characterized in that the test paper comprises a substrate (1), wherein the substrate (1) comprises a pH detection area, a chloride ion detection area, a potassium ion detection area and a sodium ion color comparison area, the pH detection area is internally provided with a pH test paper (21) and a pH color comparison card (22), the chloride ion detection area is internally provided with a chloride ion detection test paper (31) and a chloride ion color comparison card (32), the potassium ion detection area is internally provided with a potassium ion detection test paper (41) and a potassium ion color comparison card (42), and the sodium ion color comparison area is internally provided with a sodium ion color comparison card (5) combined with the chloride ion color comparison card (32) and the potassium ion color comparison card (42), so that the sodium ion content in urine can be obtained through the sodium ion color comparison card (5); ferric nitrate is immersed on the chloride ion detection test paper (31), and the potassium ion detection test paper (41) is white absorbent paper;
The sodium ion color chart (5) comprises a transverse column formed by the chloride ion color chart (32) and a longitudinal column formed by the potassium ion color chart (42), wherein the chloride ion color blocks of the transverse column are light color blocks to dark color blocks from left to right; the potassium ions in the longitudinal columns are dark color blocks to light color blocks from top to bottom;
the test paper adopts the following formula to convert to obtain the content range of sodium ions in urine:
C Urine sodium ＝C Urine chlorine -C Urinary potassium -a，
Wherein, C Urine sodium is the concentration of sodium ions in urine, C Urine chlorine is the concentration of chloride ions in urine, C Urinary potassium is the concentration of potassium ions in urine, and a is the anion gap;
The using method of the test paper comprises the following steps: (1) detecting chloride ions in urine: placing 0.5mL of urine sample into a 10mL Schlenk tube, detecting the pH value by using pH test paper, slowly adding nitric acid to neutralize urine while shaking until the pH value is 7 if the urine sample is alkaline, cooling to room temperature, adding 1mL nitric acid and 4mL mercuric thiocyanate solution, adding water to dilute to a scale, shaking uniformly, standing for 30min, dripping the reaction liquid onto the chloridion detection test paper, and comparing by using a chloridion colorimetric card;
(2) Detecting potassium ions in urine: placing 0.5mL of urine sample in a 10mL Schlenk tube, detecting the pH value by using pH test paper, slowly adding sodium hydroxide solution to neutralize urine to alkalescence while shaking if the urine sample is slightly acidic, heating at 60-80 ℃ for 5-10 minutes, cooling, adding 1mL of acetic acid, shaking uniformly, dropwise adding 1mL of sodium cobalt nitrite, dripping the reaction liquid on potassium ion detection test paper, and comparing by using a potassium ion colorimetric card;
(3) And comparing the color lump range after the chlorine ion color chart with the chlorine ion detection test paper, and combining the color lump range after the potassium ion color chart is compared with the potassium ion detection test paper to obtain the sodium content in the urine.
2. The method for using the test paper for measuring sodium content in urine according to claim 1, wherein the substrate (1) comprises an upper substrate (11) and a lower substrate (12), the upper substrate (11) and the lower substrate (12) are made of hard paper boards, the two substrates are integrally formed, and a crease is formed at the boundary.
3. The method for using the test paper for measuring sodium content in urine according to claim 2, wherein the upper substrate (11) is provided with an extension part (111), the extension part (111) is made of a hard paperboard, and a crease is formed at the boundary between the extension part and the upper substrate (11).
4. The method for using the test paper for measuring sodium content in urine according to claim 3, wherein the extension part (111) is provided with a button (112), and the lower substrate (12) is provided with a button slot (121) matched with the button (112).
5. The method for using the test paper for measuring the sodium content in urine according to claim 1, wherein the method for preparing the chloride ion colorimetric card is characterized by comprising the following steps of: preparing 0.01mg/mL sodium chloride standard solution, sequentially sucking 0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL, 12.0mL and 20.0mL of sodium chloride standard solution, respectively placing into a 50mL volumetric flask, adding 5mL of nitric acid, 5mL of ferric nitrate and 20mL of mercuric thiocyanate solution, shaking uniformly, adding water for dilution to scale, standing for 30min for color development, and dividing corresponding color ranges to obtain the chloride ion color card.
6. The method for using the test paper for measuring sodium content in urine according to claim 1, wherein the method for preparing the potassium ion colorimetric card is characterized by comprising the following steps: preparing 0.01mg/mL potassium chloride standard solution, sequentially sucking 0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL, 12.0mL and 20.0mL of potassium chloride standard solution, respectively placing the solution in a 50mL volumetric flask, shaking up 5mL of acetic acid, dropwise adding 5mL of sodium cobalt nitrite, shaking up, adding water to dilute to scale, standing for color development, and dividing corresponding color ranges to obtain the potassium ion color card.
7. The method of claim 1-6, wherein the test strip is used for rapid comparative detection of sodium content in urine.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203786040U (en) * | 2014-03-25 | 2014-08-20 | 福建中烟工业有限责任公司 | Device for determining formaldehyde content |
| CN105954279A (en) * | 2016-06-23 | 2016-09-21 | 北京大学第三医院 | Rapid detection kit for sodium chloride in urine |
| CN211179548U (en) * | 2019-08-29 | 2020-08-04 | 南方医科大学南方医院 | Test paper for determining sodium content in urine |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20060281188A1 (en) * | 2005-06-13 | 2006-12-14 | Cornell Research Foundation, Inc. | Ratiometric test strip and method |
| CN101865911A (en) * | 2010-03-16 | 2010-10-20 | 苏州市玮琪生物科技有限公司 | Urine creatine quantitative test card |
| CN106198523A (en) * | 2016-07-06 | 2016-12-07 | 哈尔滨贝贝凯尔科技发展有限公司 | Trace element detection reagent paper and preparation method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203786040U (en) * | 2014-03-25 | 2014-08-20 | 福建中烟工业有限责任公司 | Device for determining formaldehyde content |
| CN105954279A (en) * | 2016-06-23 | 2016-09-21 | 北京大学第三医院 | Rapid detection kit for sodium chloride in urine |
| CN211179548U (en) * | 2019-08-29 | 2020-08-04 | 南方医科大学南方医院 | Test paper for determining sodium content in urine |
Non-Patent Citations (1)
| Title |
|---|
| 糖尿病患者尿阴离子间隙的改变及其临床意义;齐绍康 等;《中华内分泌代谢杂志》;第8卷(第2期);第109-110页 * |
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