CN101587118A - Creatinine urine test paper and preparing method thereof - Google Patents
Creatinine urine test paper and preparing method thereof Download PDFInfo
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- CN101587118A CN101587118A CNA200910067221XA CN200910067221A CN101587118A CN 101587118 A CN101587118 A CN 101587118A CN A200910067221X A CNA200910067221X A CN A200910067221XA CN 200910067221 A CN200910067221 A CN 200910067221A CN 101587118 A CN101587118 A CN 101587118A
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Abstract
The present invention relates to a creatinine urine test paper which belongs to an in vitro diagnostic reagent. The creatinine urine test paper comprises the following components: 1000ml of 2M sodium hydroxide buffer solution with the pH value of 10.5-12.5, 2g-5g of sodium chloride or potassium chloride, 1g-7g of polyvinyl pyrrolidone, 100mg-200mg of orange G; 400mg-800mg of 3,5-dinitrobenzonic acid, and 1000ml of methanol. The creatinine urine of the invention has the following advantages: convenient use, excellent reliability, simple preparing method, simple operation and strong salt interference resistance.
Description
Technical field
The present invention is the urinalysis test strips, belongs to external diagnosis reagent.
Background technology
Creatinine in the body comprises the exogenous creatinine that absorbs and by the endogenous creatinine that generates in the body from food.Weighing apparatus is fixed relatively for the growing amount of myogenic acid anhydride in same individual every day.Because creatinine is a kind of end last metabolin of the small-molecular weight that does not combine with plasma proteins, except that being drained by the renal tubule ion channel on a small quantity, the overwhelming majority enters crude urine through glomerular filtration, is not heavily absorbed by renal tubule.If can control the picked-up of exogenous creatinine, creatinine can be used as clearance rate and checks comparatively ideal endogenous material.Therefore check the diagnosis of the variation of UCr content to kidney trouble, the variation and the result of treatment of observing disease have important value.
A kind of test paper that detects creatinine in the urine is arranged at present, and the method for use is that creatinine and copper react, again catalysis superoxide and tetramethyl benzidine generation change color.The shortcoming of the method is to be subjected to the interference of salt component big, and after eating the many food of saliferous, the salt that is excreted in the urine is just many, tests just height of creatinine value this moment.
Summary of the invention
The invention provides a kind of creatinine urine test paper and preparation method, disturb greatly, make the higher problem of measured value to solve the salt that is subjected to that exists when present use copper method is tested creatinine.The technical scheme that the present invention takes is: comprise following component:
A liquid:
The 2M sodium hydrate buffer solution, pH:10.5-12.5,1000ml,
Sodium chloride or potassium chloride 2g～5g,
Polyvinylpyrrolidone 1g～7g,
Orange G 100mg～200mg;
B liquid:
3.5-dinitrobenzoic acid 400mg～800mg,
Methyl alcohol 1000ml.
The preparation method of creatinine urine test paper of the present invention prepares after A liquid and B liquid is mixed respectively, the dissolving, soaks above-mentioned A solution with 3MM filter paper, afterwards with this 3MM filter paper in baking oven 80～100 ℃ dry down, drying time is 20～30 minutes; Then this 3MM filter paper being soaked above-mentioned B solution again, is 50～60 ℃ of oven dry down in temperature, and drying time is 15～25 minutes.
The advantage of patent of the present invention is: easy to use, reliable, the preparation method is simple, and is easy to operate, and anti-salt interference performance is strong.
Creatinine test paper of the present invention can react with the creatinine in the urine, and concentration is from 10mg/dL to 500mg/dL, and reaction color and has good reaction gradient from the lilac to the darkviolet.Salt content is higher in the urine, and it is the interference of the sodium chloride of 40000mg/L that creatinine test paper of the present invention is not subjected to concentration, and concrete test result sees Table 1.
The test result of table 1 creatinine test paper
As can be seen from the above table, the anti-salt interference performance of creatinine test paper prepared by this method is very strong.
Embodiment
Embodiment 1
A liquid:
The 2M sodium hydrate buffer solution, pH:10.5 1000ml
Sodium chloride 2g
Polyvinylpyrrolidone 1g
Orange G 100mg
B liquid:
3.5-dinitrobenzoic acid 400mg
Methyl alcohol 1000ml
Prepare after A liquid and B liquid is mixed respectively, the dissolving, soak above-mentioned A solution with 3MM filter paper, afterwards with this 3MM filter paper in baking oven 80 ℃ dry down, drying time is 30 minutes; Then this 3MM filter paper being soaked above-mentioned B solution again, is 50 ℃ of oven dry down in temperature, and drying time is 25 minutes.
Embodiment 2
A liquid:
The 2M sodium hydrate buffer solution, pH:11.5 1000ml
Sodium chloride 5g
Polyvinylpyrrolidone 7g
Orange G 150mg
B liquid:
3.5-dinitrobenzoic acid 400mg
Methyl alcohol 1000ml
Prepare after A liquid and B liquid is mixed respectively, the dissolving, soak above-mentioned A solution with 3MM filter paper, afterwards with this 3MM filter paper in baking oven 90 ℃ dry down, drying time is 25 minutes; Then this 3MM filter paper being soaked above-mentioned B solution again, is 55 ℃ of oven dry down in temperature, and drying time is 20 minutes.
Embodiment 3
A liquid:
2M sodium hydrate buffer solution (pH:12.5) 1000ml
Sodium chloride 3.5g
Polyvinylpyrrolidone 4g
Orange G 200mg
B liquid:
3.5-dinitrobenzoic acid 600mg
Methyl alcohol 1000ml
Prepare after A liquid and B liquid is mixed respectively, the dissolving, soak above-mentioned A solution with 3MM filter paper, afterwards with this 3MM filter paper in baking oven 100 ℃ dry down, drying time is 20 minutes; Then this 3MM filter paper being soaked above-mentioned B solution again, is 60 ℃ of oven dry down in temperature, and drying time is 15 minutes.
Embodiment 4
A liquid:
2M sodium hydrate buffer solution (pH:12.5) 1000ml
Sodium chloride 3.5g
Polyvinylpyrrolidone 4g
Orange G 150mg
B liquid:
3.5-dinitrobenzoic acid 800mg
Methyl alcohol 1000ml
Prepare after A liquid and B liquid is mixed respectively, the dissolving, soak above-mentioned A solution with 3MM filter paper, afterwards with this 3MM filter paper in baking oven 88 ℃ dry down, drying time is 21 minutes; Then this 3MM filter paper being soaked above-mentioned B solution again, is 54 ℃ of oven dry down in temperature, and drying time is 19 minutes.
Embodiment 5
A liquid:
The 2M sodium hydrate buffer solution, pH:11.5 1000ml
Potassium chloride 5g
Polyvinylpyrrolidone 1g
Orange G 150mg
B liquid:
3.5-dinitrobenzoic acid 400mg
Methyl alcohol 1000ml
Prepare after A liquid and B liquid is mixed respectively, the dissolving, soak above-mentioned A solution with 3MM filter paper, afterwards with this 3MM filter paper in baking oven 90 ℃ dry down, drying time is 25 minutes; Then this 3MM filter paper being soaked above-mentioned B solution again, is 55 ℃ of oven dry down in temperature, and drying time is 20 minutes.
Claims (2)
1, a kind of creatinine urine test paper is characterized in that comprising following component:
A liquid:
The 2M sodium hydrate buffer solution, pH:10.5-12.5,1000ml,
Sodium chloride or potassium chloride 2g～5g,
Polyvinylpyrrolidone 1g～7g,
Orange G 100mg～200mg;
B liquid:
3.5-dinitrobenzoic acid 400mg～800mg,
Methyl alcohol 1000ml.
2, the preparation method of creatinine urine test paper as claimed in claim 1, it is characterized in that: prepare after A liquid and B liquid are mixed respectively, dissolve, soak above-mentioned A solution with 3MM filter paper, with 80～100 ℃ of oven dry down in baking oven of this 3MM filter paper, drying time is 20～30 minutes afterwards; Then this 3MM filter paper being soaked above-mentioned B solution again, is 50～60 ℃ of oven dry down in temperature, and drying time is 15～25 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910067221 CN101587118B (en) | 2009-07-03 | 2009-07-03 | Creatinine urine test paper and preparing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910067221 CN101587118B (en) | 2009-07-03 | 2009-07-03 | Creatinine urine test paper and preparing method thereof |
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| Publication Number | Publication Date |
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| CN101587118A true CN101587118A (en) | 2009-11-25 |
| CN101587118B CN101587118B (en) | 2012-09-12 |
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| CN 200910067221 Active CN101587118B (en) | 2009-07-03 | 2009-07-03 | Creatinine urine test paper and preparing method thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101865911A (en) * | 2010-03-16 | 2010-10-20 | 苏州市玮琪生物科技有限公司 | Urine creatine quantitative test card |
| CN102661949A (en) * | 2012-04-23 | 2012-09-12 | 南京工业大学 | Test paper for detecting urine creatinine and preparation method for test paper |
| CN103630532A (en) * | 2013-11-06 | 2014-03-12 | 中国科学院苏州生物医学工程技术研究所 | Urine creatinine detection reagent strip and preparation method thereof |
| CN105705202A (en) * | 2013-09-05 | 2016-06-22 | 艾德克斯实验室公司 | Methods for detecting renal disease |
| CN107014934A (en) * | 2017-04-14 | 2017-08-04 | 广州金域医学检验中心有限公司 | Urine organic acid filter paper quality-control product and preparation method thereof |
| CN108152282A (en) * | 2017-12-14 | 2018-06-12 | 吉林省汇酉生物技术股份有限公司 | A kind of reagent for detecting urine creatinine and its application |
| CN108169222A (en) * | 2017-12-14 | 2018-06-15 | 吉林省汇酉生物技术股份有限公司 | A kind of reagent for detecting urine creatinine and its application |
| CN113376383A (en) * | 2021-06-10 | 2021-09-10 | 吉林基蛋生物科技有限公司 | Preparation method of Vc interference resistant urine creatinine test paper |
| US11422136B2 (en) | 2017-10-19 | 2022-08-23 | Idexx Laboratories, Inc. | Detection of symmetrical dimethylarginine |
| US11913942B2 (en) | 2015-02-20 | 2024-02-27 | Idexx Laboratories, Inc. | Homogenous immunoassay with compensation for background signal |
-
2009
- 2009-07-03 CN CN 200910067221 patent/CN101587118B/en active Active
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101865911A (en) * | 2010-03-16 | 2010-10-20 | 苏州市玮琪生物科技有限公司 | Urine creatine quantitative test card |
| CN102661949A (en) * | 2012-04-23 | 2012-09-12 | 南京工业大学 | Test paper for detecting urine creatinine and preparation method for test paper |
| CN105705202A (en) * | 2013-09-05 | 2016-06-22 | 艾德克斯实验室公司 | Methods for detecting renal disease |
| US11035861B2 (en) | 2013-09-05 | 2021-06-15 | Idexx Laboratories, Inc. | Methods for detecting renal disease |
| CN103630532B (en) * | 2013-11-06 | 2016-04-13 | 中国科学院苏州生物医学工程技术研究所 | A kind of urine creatinine detection reagent strip and preparation method thereof |
| CN103630532A (en) * | 2013-11-06 | 2014-03-12 | 中国科学院苏州生物医学工程技术研究所 | Urine creatinine detection reagent strip and preparation method thereof |
| US11913942B2 (en) | 2015-02-20 | 2024-02-27 | Idexx Laboratories, Inc. | Homogenous immunoassay with compensation for background signal |
| CN107014934A (en) * | 2017-04-14 | 2017-08-04 | 广州金域医学检验中心有限公司 | Urine organic acid filter paper quality-control product and preparation method thereof |
| CN107014934B (en) * | 2017-04-14 | 2020-12-08 | 广州金域医学检验中心有限公司 | Quality control product of urine organic acid filter paper and preparation method thereof |
| US11422136B2 (en) | 2017-10-19 | 2022-08-23 | Idexx Laboratories, Inc. | Detection of symmetrical dimethylarginine |
| CN108152282A (en) * | 2017-12-14 | 2018-06-12 | 吉林省汇酉生物技术股份有限公司 | A kind of reagent for detecting urine creatinine and its application |
| CN108169222A (en) * | 2017-12-14 | 2018-06-15 | 吉林省汇酉生物技术股份有限公司 | A kind of reagent for detecting urine creatinine and its application |
| CN113376383A (en) * | 2021-06-10 | 2021-09-10 | 吉林基蛋生物科技有限公司 | Preparation method of Vc interference resistant urine creatinine test paper |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101587118B (en) | 2012-09-12 |
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Address after: 130012 Changchun province high tech Zone, the River Street, No. 95, No. Applicant after: Changchun Dirui Medical Technology Co., Ltd. Address before: 130012 Changchun province high tech Zone, the River Street, No. 95, No. Applicant before: Dirui Industry Co., Ltd., Changchun |
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Address after: 130012 Changchun province high tech Zone, the River Street, No. 95, No. Patentee after: Medical Polytron Technologies Inc Address before: 130012 Changchun province high tech Zone, the River Street, No. 95, No. Patentee before: Changchun Dirui Medical Technology Co., Ltd. |
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