CN103630532B - A kind of urine creatinine detection reagent strip and preparation method thereof - Google Patents
A kind of urine creatinine detection reagent strip and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of urine creatinine detection reagent strip and preparation method thereof, this detection reagent strip comprises substrate and filter paper, filter paper takes out after filter paper fully soaks respectively in A liquid and B liquid, obtained after dry, A liquid is the 80 ~ 95v/v% ethanolic solution comprising 3.2wt% ~ 4.8wt% damping fluid and 1wt% ~ 1.5wt% surfactant; B liquid comprises 0.8wt% ~ 1.2wt%3, the methanol solution of 5-dinitrobenzoic acid and 0.008wt% ~ 0.012wt% bromcresol green.Urine creatinine detection reagent strip prepared by this case, react rapidly with the creatinine in urine in the basic conditions, creatine concentration is within the scope of 0 ~ 26.4mmol/24h, test paper presents the obvious color range from light blue to brown, be easy to visual resolution and machine-readable, method for preparing test paper is simple, easy to operate, stable performance, measurement result is accurate.
Description
Technical field
The present invention relates to urine detection field, particularly a kind of urine creatinine detection reagent strip and preparation method thereof.
Background technology
Creatinine is the product of muscle human body metabolism, is UCr when discharging with urine.UCr mainly carrys out autoblood, excretes after glomerular filtration.UCr value normal range is 8.4 ~ 13.25mmol/24h or 40 ~ 130mg/dl.The daily output of normal person's UCr is very stable, the impact of basic unable to take food thing protein content and urine volume, when UCr value occurs that significantly abnormal (raise or reduce) then reflects that renal function is subject to the damage of certain degree.So by detecting the change of UCr content, the change of disease can be controlled in time and plays important reference role to treatment clinically.
Measure that the method for creatinine has oxidase to send out, picric acid method etc., these methods all need the instrument and equipment of being correlated with and meet specially trained testing staff, complicated operation, detection speed is slow, and these special instrument equipment are not easy to carry, testing cost is expensive, Clinical practice inconvenience.Current existing a kind of test paper measuring UCr generates aubergine complex compound with 3,5-dinitrobenzoic acid and creatinine complexing under basic conditions.The method improves from picric acid method, effectively reduces the interference of false creatinine, but because test paper is in manufacturing process, strong base solution under hot conditions not only degrades test paper fiber, and the oxidation stain of 3,5-dinitrobenzoic acid can be made, thus cause background interference.
Summary of the invention
For the deficiencies in the prior art part, the invention provides a kind of urine creatinine detection reagent strip and preparation method thereof, this preparation method is simple and efficient, at normal temperatures can be dry rapidly to detection reagent strip, avoid aqueous slkali degraded test paper under high temperature, also can avoid the oxidation of 3,5-dinitrobenzoic acid simultaneously.This method cost of manufacture is cheap, and reagent strip is easy to use, is convenient to preserve, and testing result quick and precisely.
Technical scheme of the present invention is summarized as follows:
A kind of urine creatinine detection reagent strip, comprises substrate and filter paper, and described filter paper attachment on the substrate, is immersed successively in described filter paper and had A liquid and B liquid, wherein:
Described A liquid comprises: 80 ~ 95v/v% ethanolic solution of 3.2wt% ~ 4.8wt% damping fluid and 1wt% ~ 1.5wt% surfactant;
Described B liquid comprises: the methanol solution of 0.8wt% ~ 1.2wt%3,5-dinitrobenzoic acid and 0.008wt% ~ 0.012wt% bromcresol green.
Preferably, described urine creatinine detection reagent strip, is characterized in that, described damping fluid is selected from NaOH, potassium hydroxide, sodium silicate, Sodium Benzoate or its combination; Described surfactant is selected from polyvinylpyrrolidone, Pluronic F-127 lauric acid ether, polyglycol or its combination.
Preferably, described urine creatinine detection reagent strip, is characterized in that, pH=13 ~ 14 of described A liquid.
Preferably, described urine creatinine detection reagent strip, is characterized in that, described substrate material can be Corvic, polypropylene or polymethylmethacrylate.
Preferably, described urine creatinine detection reagent strip, is characterized in that, described filter paper is common quantitative filter paper or common qualitative filter paper.
A preparation method for urine creatinine detection reagent strip, its feature comprises the steps:
(1) A liquid is configured: 32 ~ 48g damping fluid and 10 ~ 15g surfactant are put into container, adds 800mL ~ 950mL absolute ethyl alcohol, add water to 1000mL constant volume subsequently;
Wherein, described damping fluid is selected from NaOH, potassium hydroxide, sodium silicate, Sodium Benzoate or its combination; Described surfactant is selected from polyvinylpyrrolidone, Pluronic F-127 lauric acid ether, polyglycol or its combination;
(2) B liquid is configured: 8 ~ 12g3,5-dinitrobenzoic acid and 0.08 ~ 0.12g bromcresol green are put into container, adds absolute methanol to 1000mL constant volume;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 20 ~ 30 DEG C, drying time is 10 ~ 20 minutes;
(4) immersed by filter paper in B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 20 ~ 40 DEG C, drying time is 10 ~ 20 minutes;
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip.
Preferably, the preparation method of described urine creatinine detection reagent strip, is characterized in that, pH=13 ~ 14 of described A liquid.
Preferably, the preparation method of described urine creatinine detection reagent strip, is characterized in that, described rectangle paper slip is of a size of 0.55 × 0.80cm.
The invention has the beneficial effects as follows: urine creatinine detection reagent strip prepared by the inventive method, in the basic conditions, 3,5-dinitrobenzoic acid can react rapidly with the creatinine in urine, creatine concentration is within the scope of 0 ~ 26.4mmol/24h, reagent strip presents the obvious color range from light blue to brown, is easy to visual resolution and machine-readable, and not by the interference of other materials in urine, reagent strip preparation method is simple, easy to operate, stable performance, measurement result is accurate.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, can implement according to this with reference to instructions word to make those skilled in the art.
Embodiment 1
The present embodiment provides a kind of urine creatinine detection reagent strip, and it prepares as follows:
(1) A liquid is configured: 32g NaOH, 10g polyglycol, 800mL absolute ethyl alcohol are put into volumetric flask, adds water to 1000mL; Through the test of pH test paper, the pH=13 of A liquid;
(2) B liquid is configured: 8g3,5-dinitrobenzoic acid, 0.08g bromcresol green are put into volumetric flask, adds absolute methanol to 1000mL;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 20 DEG C, drying time is 20 minutes;
(4) by dried filter paper immersion B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 20 DEG C, drying time is 20 minutes; .
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip, rectangle paper slip is preferably dimensioned to be 0.55 × 0.80cm.
Embodiment 2
The present embodiment provides a kind of urine creatinine detection reagent strip, and it prepares as follows:
(1) A liquid is configured: 48g NaOH, 15g polyglycol, 950mL absolute ethyl alcohol are put into volumetric flask, adds water to 1000mL; Through the test of pH test paper, the pH=14 of A liquid;
(2) B liquid is configured: 12g3,5-dinitrobenzoic acid, 0.12g bromcresol green are put into volumetric flask, adds absolute methanol to 1000mL;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes;
(4) by dried filter paper immersion B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 40 DEG C, drying time is 10 minutes; .
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip, rectangle paper slip is preferably dimensioned to be 0.55 × 0.80cm.
Embodiment 3
The present embodiment provides a kind of urine creatinine detection reagent strip, and it prepares as follows:
(1) A liquid is configured: 32g potassium hydroxide, 10g polyglycol, 800mL absolute ethyl alcohol are put into volumetric flask, adds water to 1000mL; Through the test of pH test paper, the pH=13 of A liquid;
(2) B liquid is configured: 8g3,5-dinitrobenzoic acid, 0.08g bromcresol green are put into volumetric flask, adds absolute methanol to 1000mL;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes;
(4) by dried filter paper immersion B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 40 DEG C, drying time is 10 minutes; .
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip, rectangle paper slip is preferably dimensioned to be 0.55 × 0.80cm.
Embodiment 4
The present embodiment provides a kind of urine creatinine detection reagent strip, and it prepares as follows:
(1) A liquid is configured: 48g potassium hydroxide, 15g polyglycol, 950mL absolute ethyl alcohol are put into volumetric flask, adds water to 1000mL; Through the test of pH test paper, the pH=14 of A liquid;
(2) B liquid is configured: 12g3,5-dinitrobenzoic acid, 0.12g bromcresol green are put into volumetric flask, adds absolute methanol to 1000mL;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 20 DEG C, drying time is 20 minutes;
(4) by dried filter paper immersion B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes; .
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip, rectangle paper slip is preferably dimensioned to be 0.55 × 0.80cm.
Embodiment 5
The present embodiment provides a kind of urine creatinine detection reagent strip, and it prepares as follows:
(1) A liquid is configured: 40g NaOH, 12.5g polyglycol, 875mL absolute ethyl alcohol are put into volumetric flask, adds water to 1000mL; Through the test of pH test paper, the pH=13 of A liquid;
(2) B liquid is configured: 10g3,5-dinitrobenzoic acid, 0.10g bromcresol green are put into volumetric flask, adds absolute methanol to 1000mL;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 20 DEG C, drying time is 20 minutes;
(4) by dried filter paper immersion B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes; .
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip, rectangle paper slip is preferably dimensioned to be 0.55 × 0.80cm.
Embodiment 6
The present embodiment provides a kind of urine creatinine detection reagent strip, and it prepares as follows:
(1) A liquid is configured: 16g NaOH, 16g sodium silicate, 10g polyvinylpyrrolidone, 800mL absolute ethyl alcohol are put into volumetric flask, adds water to 1000mL; Through the test of pH test paper, the pH=13 of A liquid;
(2) B liquid is configured: 8g3,5-dinitrobenzoic acid, 0.08g bromcresol green are put into volumetric flask, adds absolute methanol to 1000mL;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes;
(4) by dried filter paper immersion B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 20 DEG C, drying time is 20 minutes; .
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip, rectangle paper slip is preferably dimensioned to be 0.55 × 0.80cm.
Embodiment 7
The present embodiment provides a kind of urine creatinine detection reagent strip, and it prepares as follows:
(1) A liquid is configured: 24g NaOH, 24g sodium silicate, 15g polyvinylpyrrolidone, 950mL absolute ethyl alcohol are put into volumetric flask, adds water to 1000mL; Through the test of pH test paper, the pH=14 of A liquid;
(2) B liquid is configured: 12g3,5-dinitrobenzoic acid, 0.12g bromcresol green are put into volumetric flask, adds absolute methanol to 1000mL;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes;
(4) by dried filter paper immersion B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes; .
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip, rectangle paper slip is preferably dimensioned to be 0.55 × 0.80cm.
Embodiment 8
The present embodiment provides a kind of urine creatinine detection reagent strip, and it prepares as follows:
(1) A liquid is configured: 16g potassium hydroxide, 16g Sodium Benzoate, 10g polyvinylpyrrolidone, 800mL absolute ethyl alcohol are put into volumetric flask, adds water to 1000mL; Through the test of pH test paper, the pH=13 of A liquid;
(2) B liquid is configured: 8g3,5-dinitrobenzoic acid, 0.08g bromcresol green are put into volumetric flask, adds absolute methanol to 1000mL;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes;
(4) by dried filter paper immersion B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes; .
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip, rectangle paper slip is preferably dimensioned to be 0.55 × 0.80cm.
Embodiment 9
The present embodiment provides a kind of urine creatinine detection reagent strip, and it prepares as follows:
(1) A liquid is configured: 24g potassium hydroxide, 24g Sodium Benzoate, 15g polyvinylpyrrolidone, 950mL absolute ethyl alcohol are put into volumetric flask, adds water to 1000mL; Through the test of pH test paper, the pH=14 of A liquid;
(2) B liquid is configured: 12g3,5-dinitrobenzoic acid, 0.12g bromcresol green are put into volumetric flask, adds absolute methanol to 1000mL;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes;
(4) by dried filter paper immersion B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes; .
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip, rectangle paper slip is preferably dimensioned to be 0.55 × 0.80cm.
Embodiment 10
The present embodiment provides a kind of urine creatinine detection reagent strip, and it prepares as follows:
(1) A liquid is configured: 32g sodium silicate, 10g Pluronic F-127 lauric acid ether, 800mL absolute ethyl alcohol are put into volumetric flask, adds water to 1000mL; Through the test of pH test paper, the pH=13 of A liquid;
(2) B liquid is configured: 8g3,5-dinitrobenzoic acid, 0.08g bromcresol green are put into volumetric flask, adds absolute methanol to 1000mL;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes;
(4) by dried filter paper immersion B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes; .
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip, rectangle paper slip is preferably dimensioned to be 0.55 × 0.80cm.
Embodiment 11
The present embodiment provides a kind of urine creatinine detection reagent strip, and it prepares as follows:
(1) A liquid is configured: 48g sodium silicate, 15g Pluronic F-127 lauric acid ether, 950mL absolute ethyl alcohol are put into volumetric flask, adds water to 1000mL; Through the test of pH test paper, the pH=14 of A liquid;
(2) B liquid is configured: 12g3,5-dinitrobenzoic acid, 0.12g bromcresol green are put into volumetric flask, adds absolute methanol to 1000mL;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 20 DEG C, drying time is 20 minutes;
(4) by dried filter paper immersion B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes; .
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip, rectangle paper slip is preferably dimensioned to be 0.55 × 0.80cm.
Embodiment 12
The present embodiment provides a kind of urine creatinine detection reagent strip, and it prepares as follows:
(1) A liquid is configured: 32g Sodium Benzoate, 10g Pluronic F-127 lauric acid ether, 800mL absolute ethyl alcohol are put into volumetric flask, adds water to 1000mL; Through the test of pH test paper, the pH=13 of A liquid;
(2) B liquid is configured: 8g3,5-dinitrobenzoic acid, 0.08g bromcresol green are put into volumetric flask, adds absolute methanol to 1000mL;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 20 DEG C, drying time is 20 minutes;
(4) by dried filter paper immersion B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 20 DEG C, drying time is 20 minutes; .
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip, rectangle paper slip is preferably dimensioned to be 0.55 × 0.80cm.
Embodiment 13
The present embodiment provides a kind of urine creatinine detection reagent strip, and it prepares as follows:
(1) A liquid is configured: 48g Sodium Benzoate, 15g Pluronic F-127 lauric acid ether, 950mL absolute ethyl alcohol are put into volumetric flask, adds water to 1000mL; Through the test of pH test paper, the pH=14 of A liquid;
(2) B liquid is configured: 12g3,5-dinitrobenzoic acid, 0.12g bromcresol green are put into volumetric flask, adds absolute methanol to 1000mL;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 30 DEG C, drying time is 10 minutes;
(4) by dried filter paper immersion B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 40 DEG C, drying time is 10 minutes; .
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip, rectangle paper slip is preferably dimensioned to be 0.55 × 0.80cm.
Embodiment 14
The preparation method of standard color comparison card:
(1) the creatinine standard solution of 0mmol/L, 4.4mmol/L, 8.8mmol/L, 17.6mmol/L, 26.4mmol/L is configured;
(2) be dipped in respectively in five kinds of above-mentioned creatinine standard solution by the detection reagent strip that above-described embodiment 1 ~ 13 prepares, take out, dry after 2 seconds, after 1 minute, colour developing completely;
(3) prepare colorimetric card according to the colour developing situation of each test paper, the color range of colorimetric card is that pale blue arrives 5 deeply brown color ranges.
Embodiment 15
Urine creatinine detection reagent strip using method:
Detection reagent strip embodiment 1 ~ 13 prepared immerses in liquid to be measured, take out after 2 seconds, dry, after 1 minute, colour developing completely, compare with standard color comparison card subsequently, be the concentration of liquid to be measured with the concentration corresponding to the color detected on reagent strip on the immediate standard color comparison card of color, if between two color ranges, then can estimate and read creatine concentration.
Embodiment 16
The using method of urine creatinine detection reagent strip on instrument:
(1) the creatinine standard solution of 0mmol/L, 4.4mmol/L, 8.8mmol/L, 17.6mmol/L, 26.4mmol/L is configured;
(2) the detection reagent strip that embodiment 1 ~ 13 prepares is dipped in respectively in five kinds of above-mentioned creatinine standard solution;
(3) Urine Analyzer is used to measure the reflectivity of 1 minute, according to 5 point-rendering typical curves;
(4) when using, immersed in liquid to be measured by the UCr reagent strip prepared take out according to embodiment, use Urine Analyzer to measure reflectivity after 1 minute, the concentration that the reflectivity recorded is corresponding with on typical curve is liquid creatine concentration to be measured.
Although embodiment of the present invention are open as above, but it is not restricted to listed in instructions and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details.
Claims (5)
1. a urine creatinine detection reagent strip, comprises substrate and filter paper, and described filter paper attachment on the substrate, is characterized in that, immersing successively in described filter paper has A liquid and B liquid, wherein:
Described A liquid comprises: 80 ~ 95v/v% ethanolic solution of 3.2wt% ~ 4.8wt% damping fluid and 1wt% ~ 1.5wt% surfactant;
Described B liquid comprises: the methanol solution of 0.8wt% ~ 1.2wt%3,5-dinitrobenzoic acid and 0.008wt% ~ 0.012wt% bromcresol green;
Described damping fluid is selected from potassium hydroxide, sodium silicate, Sodium Benzoate or its combination; Described surfactant is selected from Pluronic F-127 lauric acid ether, polyglycol or its combination;
PH=13 ~ 14 of described A liquid.
2. urine creatinine detection reagent strip according to claim 1, is characterized in that, described substrate material is Corvic, or polypropylene, or polymethylmethacrylate.
3. urine creatinine detection reagent strip according to claim 1, is characterized in that, described filter paper is common quantitative filter paper or common qualitative filter paper.
4. a preparation method for urine creatinine detection reagent strip, its feature comprises the steps:
(1) A liquid is configured: 32 ~ 48g damping fluid and 10 ~ 15g surfactant are put into container, adds 800mL ~ 950mL absolute ethyl alcohol, add water to 1000mL constant volume subsequently;
Wherein, described damping fluid is selected from NaOH, potassium hydroxide, sodium silicate, Sodium Benzoate or its combination; Described surfactant is selected from polyvinylpyrrolidone, Pluronic F-127 lauric acid ether, polyglycol or its combination;
(2) B liquid is configured: 8 ~ 12g3,5-dinitrobenzoic acid and 0.08 ~ 0.12g bromcresol green are put into container, adds absolute methanol to 1000mL constant volume;
(3) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 20 ~ 30 DEG C, drying time is 10 ~ 20 minutes;
(4) immersed by filter paper in B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 20 ~ 40 DEG C, drying time is 10 ~ 20 minutes;
(5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine creatinine detection reagent strip;
PH=13 ~ 14 of described A liquid.
5. the preparation method of urine creatinine detection reagent strip according to claim 4, is characterized in that, described rectangle paper slip is of a size of 0.55 × 0.80cm.
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| CN103901034B (en) * | 2014-04-29 | 2016-07-06 | 中国科学院苏州生物医学工程技术研究所 | A kind of detectable bar detecting microalbumin in urine and preparation method thereof |
| CN104568931A (en) * | 2014-12-24 | 2015-04-29 | 中国科学院苏州生物医学工程技术研究所 | Reagent strip for detecting content of creatinine in urine and preparation method of reagent strip |
| CN108693174A (en) * | 2017-04-05 | 2018-10-23 | 中国科学院大连化学物理研究所 | Thiocarbamates pesticide colorimetric inspects paper and its preparation method and application soon |
| CN109668875A (en) * | 2017-10-13 | 2019-04-23 | 中国科学院大连化学物理研究所 | It is a kind of for quickly detecting the test paper of pesticide sevin |
| CN108169222A (en) * | 2017-12-14 | 2018-06-15 | 吉林省汇酉生物技术股份有限公司 | A kind of reagent for detecting urine creatinine and its application |
| CN108152282B (en) * | 2017-12-14 | 2020-12-25 | 吉林省汇酉生物技术股份有限公司 | Reagent for detecting urine creatinine and application thereof |
| CN110954530A (en) * | 2019-11-27 | 2020-04-03 | 迪瑞医疗科技股份有限公司 | Dry chemical test paper for detecting urine creatinine and preparation method thereof |
| CN113376383A (en) * | 2021-06-10 | 2021-09-10 | 吉林基蛋生物科技有限公司 | Preparation method of Vc interference resistant urine creatinine test paper |
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