CN206095952U - Rapid detection kit - Google Patents
Rapid detection kit Download PDFInfo
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- CN206095952U CN206095952U CN201621123565.XU CN201621123565U CN206095952U CN 206095952 U CN206095952 U CN 206095952U CN 201621123565 U CN201621123565 U CN 201621123565U CN 206095952 U CN206095952 U CN 206095952U
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- drop bottle
- reagent
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- disposable
- reagent drop
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Abstract
The utility model provides a rapid detection kit, includes that box body, hinge, lid, first card, second card, urase inspect note, second reagent drop bottle, first reagent drop bottle, disposable plastics straw and disposable round bottom centrifuge tube soon, the box body has the lid through hinge connection, paste the lid inboard has first card and second card, divide into four draw -in grooves in the box body, being provided with the urase in the box body and inspecting note, second reagent drop bottle, first reagent drop bottle, disposable plastics straw and disposable round bottom centrifuge tube soon, second reagent drop bottle and first reagent drop bottle are located same draw -in groove, and two at least disposable plastics straws are located same draw -in groove, and two at least disposable round bottom centrifuge tubes are located same draw -in groove, the splendid attire has a 0.4gL phenol red indicator in the first reagent drop bottle, the splendid attire has 10% urea solution in the second reagent drop bottle, the utility model relates to a color development is quick, accurate, the shelf life is long and kit and test paper are examined soon to the urase low toxicity.
Description
Technical field
This utility model belongs to test kit, specially a kind of quick testing reagent kit.
Background technology
Though bean milk is rich in nutrition, the anti-battalion during numerous scholars are to Semen sojae atricolor on this point of toxicity based on the bean milk not boiled
The foster factor is all conducted extensive research.Antinutritional factor (antinutritional factors, ANF) are referred to nutrient
Digestion, absorption and the utilization of matter has a negative impact and makes humans and animals produce the material of untoward reaction.Semen sojae atricolor and its product
In contain various antinutritional factor, including protease inhibitor, urase, agglutinin, phytic acid, soybean oligo saccharide, soybean antigen
Albumen, lipoxidase etc..Wherein urase belongs to thermal instability antinutritional factor, generally without toxic action, but
Under the conditions of the suitable moisture of stomach enteral, temperature, pH, the urase of raw soybean can be activated, rapidly by part nitrogen in Semen sojae atricolor
Compound decomposes ammonification, and the presence of substantial amounts of ammonia can cause body ammonia metabolism obstacle or poisoning, in causing Nausea and vomiting and diarrhoea etc.
Toxication shape.Many research report it has proven convenient that in bean product urase deactivation temperature higher than antinutritional factor deactivation temperature, together
When to detect this kind of anti-nutrition because difficulty is high, some antinutritional factor even without feasible detection method, hence with urase
Test can differentiate that the antinutritional factor such as trypsin inhibitor have degree, can act also as judging the standard of bean milk degree of raw and cooked.
Urase is relatively unstable as a kind of biochemical substances, and experiment shows that 85 DEG C of positive bean milk of urase are little in insulation half
When after urase it is deactivatable.Therefore produce that an energy is quick, accurate, with low cost and quick detection kit of nonhazardouss, should
Quality used in Homemade bean milk, the commercially available bean milk in curbside and production is very meaningful shutting.Inspection to urase at present
Survey method is less, mainly the Na Shi optical colorimetries and photoelectric colorimetry of GB：
National Standard Method：The ammonia that carbamide is produced is hydrolyzed using urase, under proper condition can be with the potassiomercuric iodide in nessler reagent
Double salt effect generates the double hydrargyrum ammoniums of iodate of brown.National Standard Method sensitivity is higher, but can only qualitative detection, and process is complicated, takes
Also it is longer.
Photoelectric colorimetry：Under certain condition, hydrolyzable carbamide generates ammonium to urase, and in the basic conditions, ammonium salt is changed into
For ammonia, it generates yellowish-brown complex with nessler reagent reaction, and its colourity is directly proportional to urease activity.Can using photoelectric colorimetry
Time-consuming for the height of quantitative determination urease activity, its assay method good stability, reliable results, but whole operation, in 2h or so,
Inconvenience is applied to field test.And because enzymatic activity is larger by temperature, time effects, from quantitative consideration, operating process is to sample
Add carbamide, water bath heat preservation to urase is inactivated in product, bath temperature, time must be accurate.
The problem that simultaneously presently commercially available urase quick detection kit is present has：1. the test kit shelf-life is shorter；2. show
The color time is longer, and what is had even needs 15-30 minutes, it is impossible to reach the purpose of fast inspection；3. develop the color unobvious, it is difficult to which observation is sentenced
It is fixed；4. reagent has stronger corrosivity；5. the urase also not occurred on the market inspects soon paper.
Research now both at home and abroad with regard to urase Fast Detection Technique is very few, is substantially also to pass through National Standard Method in the lab
Measure to urease activity, and fast check reagent box on the market more or less exist short shelf-life durations or response time length or
Person's reagent has the shortcomings that corrosivity, and urase to inspect paper soon not yet ripe.If thus it is quick, bright to develop a kind of colour developing
Aobvious, the fast check reagent box of urase and reagent paper of long shelf-life and low toxicity are being applied to sampling observation, supermarket, the dining room in market circulation field
Or campus periphery, city street dissipate and sell and the virose detection of Homemade bean milk, the supervision, broad masses for food safety
Food safety is ensured and is significant.
Utility model content
The purpose of this utility model is to provide a kind of quick testing reagent kit, in solving the problems, such as background technology.
This utility model employs the following technical solutions realization：
A kind of quick testing reagent kit, including box body, hinge, lid, the first card, the second card, urase inspect soon paper
Bar, the second reagent drop bottle, the first reagent drop bottle, disposable plastic tube and disposable round bottom centrifuge tube, the box body is by closing
Page is connected with lid, and the lid inner side is pasted with the first card and the second card, four draw-in grooves are divided in the box body, described
Urase is provided with box body and inspects paper slip, the second reagent drop bottle, the first reagent drop bottle, disposable plastic tube and disposable circle soon
Bottom centrifuge tube, the second reagent drop bottle and the first reagent drop bottle are located in same draw-in groove, and at least two disposable plastic tubes are located at
Same draw-in groove, at least two disposable round bottom centrifuge tubes are located at same draw-in groove, and in the first reagent drop bottle 0.4g/L is loaded with
Phenol red indicator, is loaded with 10% urea liquid in the second reagent drop bottle.
In this utility model, on the disposable plastic tube scale is provided with.
In this utility model, on the disposable round bottom centrifuge tube scale is provided with.
In this utility model, the volume of the disposable plastic tube is 1mL.
In this utility model, the volume of the disposable round bottom centrifuge tube is 2mL.
In this utility model, urease reagents box operation instructions are identified with first card.
In this utility model, urease activity reagent paper colorimetric card is identified with second card and urease activity shows the time
To picture ID.
Beneficial effect：This utility model is a kind of quick, accurate, fast check reagent box of the urase of long shelf-life and low toxicity that develops the color
And reagent paper.This utility model finds that the urase energy catalyzing urea in bean milk forms ammonium hydroxide, so that phenol by studying
Red indicator is changed into red from yellow, and the activity of color and urase is into positive correlation.This utility model is based on the studies above, is made that
A kind of test kit and reagent paper for using urease activity in a quick detection bean milk, the test kit and reagent paper are applicable to detect bean milk life
The important indicator of ripe degree --- urease activity, the quick detection for urease activity provides a kind of new method.
Test kit of the present utility model and reagent paper can execute-in-place, it is aobvious by contrast without the need for other special reagents and instrument
Colour atla, can draw testing result at one minute or so, and, step many compared to national standard method instrument reagent is numerous and diverse, this practicality
New have the advantages that convenient, directly perceived, quick, and user can be helped to judge the urease activity in bean milk rapidly.
Test kit of the present utility model and reagent paper at normal temperatures can be to use, under conditions of temperature is for 20 DEG C -40 DEG C
Using effect preferably, and needs to be incubated 20min in 40 DEG C of water-baths compared to national standard method, and other fast detecting methods need to use
Handss seal hot more than 15min, of the present utility model using easier.
This utility model finds that the sensitivity that test kit is detected to urease activity is high, quick compared to other by studying
Detection method, phenol red colour developing becomes apparent from, and detection urease activity is：It is strong positive, secondary strong positive, the positive, weakly positive, negative
During different samples, color has significant difference, and (the weak sun of urase is verified as with National Standard Method even if raw soya-bean milk is diluted to into 0.1%
Property), remain to clearly identify the difference of color, illustrate that the test kit has higher accuracy and susceptiveness.
Test kit of the present utility model and method for preparing test paper are simple, it is easy to operate, and are adapted to large batch of production, Er Qiewu
Need to be preserved by special method Jing row, be preserved in normal temperature drying condition.
Description of the drawings
Fig. 1 is structural representation of the present utility model.
Specific embodiment
In order that technological means, creation characteristic, reached purpose and effect that this utility model is realized are easy to understand, under
Face combines and is specifically illustrating, and this utility model is expanded on further.
Referring to Fig. 1, a kind of structural representation of quick testing reagent kit, a kind of quick testing reagent kit, including box body 1,
Hinge 2, lid 3, the first card 4, the second card 5, urase inspect soon paper slip 6, the second reagent drop bottle 7, the first reagent drop bottle 8,
Disposable plastic tube 9 and disposable round bottom centrifuge tube 10, the box body 1 is connected with lid 3 by hinge 2, in the lid 3
Side is pasted with the first card 4 and the second card 5, is divided into that to be provided with urase in four draw-in grooves, the box body 1 fast in the box body 1
Paper slip 6, the second reagent drop bottle 7, the first reagent drop bottle 8, disposable plastic tube 9 and disposable round bottom centrifuge tube 10 are inspected, the
Two reagent drop bottles 7 and the first reagent drop bottle 8 are located in same draw-in groove, and at least two disposable plastic tubes 9 are located at same draw-in groove,
At least two disposable round bottom centrifuge tubes 10 are located in same draw-in groove, the first reagent drop bottle 8 and are loaded with 0.4g/L phenol reds
10% urea liquid is loaded with indicator, the second reagent drop bottle 7, quarter is provided with the disposable plastic tube 9
Degree, is provided with scale on the disposable round bottom centrifuge tube 10, the volume of the disposable plastic tube 9 is 1mL, it is described once
Property round bottom centrifuge tube 10 volume be 2mL, be identified with urease reagents box operation instructions on first card 4, described second
Urease activity reagent paper colorimetric card is identified with card 5 and urease activity shows time comparison card.
When using, this utility model surveys urase qualitatively principle according to National Standard Method, by carbamide under the catalytic action of urase
Ammonium carbonate is generated, the unstable generation ammonia that decomposes of ammonium carbonate causes the change of environment pH, so that acid-base indicator discoloration：
(NH4)2CO3+2NaOH→NaCO3+NH4OH
NH4OH→NH3+H2O → acid-base indicator discoloration
And then it is quick, accurate to set up one or more, it is adaptable to the new technique of urase Site Detection in bean milk.
In this utility model, in the first reagent drop bottle 8 0.4g/L phenol red indicators, 0.4g/L phenol reds are loaded with
The preparation method of indicator is as follows：0.1g phenol reds are weighed, 1.43mL 0.1mol/L sodium hydroxide solutions is added, in mortar
With dissolution solution, in being transferred to 250mL volumetric flasks, plus distilled water is to scale, shakes up standby for grinding.
In this utility model, 10% urea liquid is loaded with the second reagent drop bottle 7,10% urea liquid
Preparation method is as follows：10g urea solids are weighed, with distillation water dissolution 100mL is settled to.
Embodiment 1
When using 1mL bean milk sample is drawn to disposable round bottom centrifuge tube with the disposable plastic tube 9 provided in box body 1
In 10, shaken to after disposable round bottom centrifuge tube 10 uniformly with the drop phenol red indicator of 8 Deca of the first reagent bottle 1, observe bean milk
Color, then from 7 Deca of the second reagent drop bottle 1 urea liquid is dripped to after disposable round bottom centrifuge tube 10, after fully shaking up, observe bean
Change of the pulp color after Deca urea liquid, and bean milk is recorded from the Deca urea liquid metachroic time.Carried according to table 1
For developing time card, the general temperature and time of colour developing for compareing environment may determine that the urease activity of the sample is strong
It is weak.If simultaneously bean milk color does not change always, judge that bean milk urease activity is negative.
Table 1：The developing time card of different urease activity bean milk under different temperatures
| Time/min | Pole weakly positive | Weakly positive | It is positive | (secondary) strong positive |
| 10℃ | 15 | 5 | 2 | <0.5 |
| 20℃ | 8 | 2 | 1 | <0.5 |
| 30℃ | 5 | 1 | 0.5 | <0.3 |
| 40℃ | 3 | 0.5 | 0.2 | <0.2 |
Embodiment 2
When using, phenol red indicator is prepared：0.1g phenol reds are weighed, adds 1.43mL0.1mol/L sodium hydroxide molten
Liquid, grinds with dissolution solution in mortar, and in being transferred to volumetric flask, plus distilled water is to scale, shakes up standby；Configuration mixed solution：
1g/L carbamide -0.4g/L phenol red solution：0.25g carbamide is weighed, in being dissolved in the 0.4g/L phenol red solution of 250mL, is urinated
Element-phenol red solution；Little square filter paper will be cut into be fixed on the white plastic paper of strip, reagent paper end is immersed in above-mentioned
In obtained mixed solution in 5min, it is subsequently placed at shady and cool ventilation and is dried, obtains urase and inspect paper slip 6 soon, can be straight when using
To connect inspect soon urase the one end of paper slip 6 and immerse in bean milk sample to be measured to be taken out after 1s and get rid of immediately unnecessary bean milk sample in order to avoid bean milk
Color is disturbed, it is also possible to is dropped in urase with the absorption 1-2 drop bean milk samples of disposable plastic tube 9 and is inspected soon on paper slip 6, observes urea
Enzyme inspects soon the color change of paper slip 6.Require with 20min observe color change control give must develop the color card reading phase
The urease activity answered is strong and weak.Because even being positive, the redness shown after more than 20min-30min is also returned and takes off into reagent paper
The yellow of itself, causes result distortion.
Ultimate principle of the present utility model and principal character and advantage of the present utility model, one's own profession has been shown and described above
The technical staff of industry it should be appreciated that this utility model is not restricted to the described embodiments, described in above-described embodiment and description
Simply illustrate principle of the present utility model, on the premise of without departing from this utility model spirit and scope, this utility model is also
Various changes and modifications are had, these changes and improvements are both fallen within the range of claimed this utility model, this utility model
Claimed scope is by appending claims and its equivalent thereof.
Claims (7)
1. a kind of quick testing reagent kit, including box body, hinge, lid, the first card, the second card, urase inspect soon paper slip,
Second reagent drop bottle, the first reagent drop bottle, disposable plastic tube and disposable round bottom centrifuge tube, it is characterised in that the box
Body has lid by gemel connection, and the lid inner side is pasted with the first card and the second card, four are divided in the box body
Draw-in groove, is provided with urase and inspects paper slip, the second reagent drop bottle, the first reagent drop bottle, disposable plastic tube soon in the box body
With disposable round bottom centrifuge tube, the second reagent drop bottle and the first reagent drop bottle be located in same draw-in groove, at least two disposable modelings
Material suction pipe is located at same draw-in groove, and at least two disposable round bottom centrifuge tubes are located in same draw-in groove, the first reagent drop bottle and contain
Equipped with 0.4g/L phenol red indicators, 10% urea liquid is loaded with the second reagent drop bottle.
2. a kind of quick testing reagent kit according to claim 1, it is characterised in that set on the disposable plastic tube
It is equipped with scale.
3. a kind of quick testing reagent kit according to claim 1, it is characterised in that on the disposable round bottom centrifuge tube
It is provided with scale.
4. a kind of quick testing reagent kit according to claim 1, it is characterised in that the appearance of the disposable plastic tube
Product is 1mL.
5. a kind of quick testing reagent kit according to claim 1, it is characterised in that the disposable round bottom centrifuge tube
Volume is 2mL.
6. a kind of quick testing reagent kit according to claim 1, it is characterised in that be identified with urea on first card
Enzyme reagent kit operation instructions.
7. a kind of quick testing reagent kit according to claim 1, it is characterised in that be identified with urea on second card
Enzymatic activity reagent paper colorimetric card and urease activity show time comparison card.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621123565.XU CN206095952U (en) | 2016-10-14 | 2016-10-14 | Rapid detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621123565.XU CN206095952U (en) | 2016-10-14 | 2016-10-14 | Rapid detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206095952U true CN206095952U (en) | 2017-04-12 |
Family
ID=58483219
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621123565.XU Expired - Fee Related CN206095952U (en) | 2016-10-14 | 2016-10-14 | Rapid detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206095952U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112858276A (en) * | 2021-01-25 | 2021-05-28 | 郑州欧柯奇仪器制造有限公司 | Soybean milk maturity rapid test card and detection method thereof |
-
2016
- 2016-10-14 CN CN201621123565.XU patent/CN206095952U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112858276A (en) * | 2021-01-25 | 2021-05-28 | 郑州欧柯奇仪器制造有限公司 | Soybean milk maturity rapid test card and detection method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170412 Termination date: 20171014 |