CN104568931A - Reagent strip for detecting content of creatinine in urine and preparation method of reagent strip - Google Patents
Reagent strip for detecting content of creatinine in urine and preparation method of reagent strip Download PDFInfo
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- CN104568931A CN104568931A CN201410810764.7A CN201410810764A CN104568931A CN 104568931 A CN104568931 A CN 104568931A CN 201410810764 A CN201410810764 A CN 201410810764A CN 104568931 A CN104568931 A CN 104568931A
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Abstract
The invention discloses a reagent strip for detecting the content of creatinine in urine and a preparation method of the reagent strip. The reagent strip comprises a base plate and a filter paper piece, wherein the filter paper piece is immersed with a solution A and a solution B in sequence; the solution A contains beta-glycerophosphate, sodium citrate, copper sulfate and a surfactant, and the volume is kept constant by deionized water; and the solution B contains reductive chromogen, absolute ethyl alcohol, an antioxidant, peroxide and a surfactant, and the volume is kept constant by chloroform. According to the reagent strip, the antioxidant is added to the system so that the false positive rate of the reagent strip is reduced; meanwhile, the reagent strip can be stored for a long period of time without losing efficiency; beta-glycerophosphate is used for replacing a Tris buffering solution system and the pH value of the system is controlled to be 6-7; the peroxide is introduced so that the reagent strip is very sensitive to a developing reaction of creatinine; the developing is rapid and clear; and the gradual change difference of the colors of the reagent strips in creatinine with different concentrations is obvious, and visual judgment and machine-reading judgment of operators are facilitated.
Description
Technical field
The present invention relates to urine detection field, particularly a kind ofly detect reagent strip of creatinine content in urine and preparation method thereof.
Background technology
Creatinine is the product of muscle human body metabolism, is UCr when discharging with urine.UCr mainly carrys out autoblood, excretes after glomerular filtration.UCr value normal range is 8.4 ~ 13.25mmol/24h or 40 ~ 130mg/dl.The daily output of normal person's UCr is very stable, the impact of basic unable to take food thing protein content and urine volume, when UCr value occurs that significantly abnormal (raise or reduce) then reflects that renal function is subject to the damage of certain degree.So by detecting the change of UCr content, the change of disease can be controlled in time and plays important reference role to treatment clinically.
Measure that the method for creatinine has oxidase to send out, picric acid method etc., these methods all need the instrument and equipment of being correlated with and meet specially trained testing staff, complicated operation, detection speed is slow, and these special instrument equipment are not easy to carry, testing cost is expensive, Clinical practice inconvenience.Current existing a kind of test paper measuring UCr generates aubergine complex compound with 3,5-dinitrobenzoic acid and creatinine complexing under basic conditions.The method improves from picric acid method, effectively reduces the interference of false creatinine, but because test paper is in manufacturing process, strong base solution under hot conditions not only degrades test paper fiber, and the oxidation stain of 3,5-dinitrobenzoic acid can be made, thus cause background interference.
In addition, Chinese patent 201210121232.3 discloses a kind of urine creatinine Test paper and preparation method thereof, but there is some deficiency following in the technical scheme that this patent proposes: TMB reagent 1) used is easily oxidized, makes reagent false positive rate higher, not easily preserves for a long time; 2) under Tris buffer solution system, the chromogenic reaction of test paper to creatinine is insensitive, and the change of color is not obvious, and in the creatinine of variable concentrations, the gradual differences of test paper color is very little, and this brings severe jamming to the visual judgement of operator and machine-readable judgement.
Summary of the invention
The technical scheme that the present invention is directed to Chinese patent 201210121232.3 proposes improvement, its objective is and solves its insurmountable difficult problem.
Technical scheme of the present invention is summarized as follows:
Detect a reagent strip for creatinine content in urine, comprise substrate and filter paper, described filter paper attachment on the substrate, is immersed successively in described filter paper and is had A liquid and B liquid, wherein:
Described in every 1000mL, A liquid comprises: the surfactant of the β-phosphoglycerol disodium of 200 ~ 250g, the sodium citrate of 2.5 ~ 3.5g, the copper sulphate of 1.2 ~ 2.0g and 0.1 ~ 0.5g, and uses deionized water constant volume;
Described in every 1000mL, B liquid comprises: the surfactant of the absolute ethyl alcohol of 1.2 ~ 1.8g reductibility chromogen, 80 ~ 120g, the antioxidant of 2.6 ~ 3.2g, the superoxide of 2 ~ 3g and 4 ~ 8g, and uses chloroform constant volume.
Preferably, the reagent strip of creatinine content in described detection urine, described antioxidant is selected from oxine, 2-hydroxyquinoline, 8-methylquinoline or its combination, and described reductibility chromogen is TMB.
Preferably, the reagent strip of creatinine content in described detection urine, drips hydrochloric acid in described A liquid, and makes pH=6 ~ 7 of described A liquid.
Preferably, the reagent strip of creatinine content in described detection urine, described substrate material is Corvic, or polypropylene, or polymethylmethacrylate.
Preferably, the reagent strip of creatinine content in described detection urine, described surfactant is selected from polyvinylpyrrolidone, polyglycol or its combination.
Preferably, the reagent strip of creatinine content in described detection urine, described superoxide is selected from hydrogen phosphide cumene, di-isopropylbenzene hydroperoxide, tert-butyl hydroperoxide or its combination.
As a preparation method for the reagent strip of creatinine content in detection urine arbitrary in claim 1 ~ 6, comprise the steps:
Step 1) configuration A liquid: the surfactant adding the β-phosphoglycerol disodium of 200 ~ 250g, the sodium citrate of 2.5 ~ 3.5g, the copper sulphate of 1.2 ~ 2.0g and 0.1 ~ 0.5g in the A liquid of every 1000mL respectively, use deionized water constant volume;
Step 2) configuration B liquid: the surfactant adding 1.2 ~ 1.8g reductibility chromogen, the absolute ethyl alcohol of 80 ~ 120g, the antioxidant of 2.6 ~ 3.2g, the superoxide of 2 ~ 3g and 4 ~ 8g in the B liquid of every 1000mL respectively, use chloroform constant volume;
Step 3) filter paper is immersed in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 60 ~ 80 DEG C, drying time is 5 ~ 20 minutes;
Step 4) filter paper is immersed in B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 40 ~ 60 DEG C, drying time is 3 ~ 10 minutes;
Step 5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make the reagent strip detecting creatinine content in urine.
Preferably, in described detection urine, the preparation method of the reagent strip of creatinine content, is also included in step 1) in make pH=6 ~ 7 of described A liquid by dripping hydrochloric acid.
Preferably, the preparation method of the reagent strip of creatinine content in described detection urine, described antioxidant is selected from oxine, 2-hydroxyquinoline, 8-methylquinoline or its combination, described reductibility chromogen is 3,3 ', 5,5 '-tetramethyl benzidine, described superoxide is selected from hydrogen phosphide cumene, di-isopropylbenzene hydroperoxide, tert-butyl hydroperoxide or its combination.
Preferably, the preparation method of the reagent strip of creatinine content in described detection urine, described surfactant is selected from polyvinylpyrrolidone, polyglycol or its combination.
The invention has the beneficial effects as follows: 1) this case by adding antioxidant in system, effectively extends the shelf-life of chromogen, and reduce the false positive rate of reagent strip, make reagent strip to preserve for a long time simultaneously and do not lose efficacy; 2) use β-phosphoglycerol disodium instead and substitute Tris buffer solution system, and system pH is controlled between 6 ~ 7, introduce superoxide simultaneously, the chromogenic reaction of this reagent strip to creatinine is made to become very sensitive, colour developing is clearly demarcated rapidly, and in the creatinine of variable concentrations, the gradual differences of reagent strip color is obvious, is conducive to the visual judgement to operator and machine-readable judgement.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, can implement according to this with reference to instructions word to make those skilled in the art.
Embodiment 1
The reagent strip of creatinine content in one detection urine, comprises substrate and filter paper, prepares as follows:
Step 1) configuration A liquid: the polyvinylpyrrolidone adding the β-phosphoglycerol disodium of 200g, the sodium citrate of 2.5g, the copper sulphate of 1.2g and 0.1g in the A liquid of every 1000mL respectively, use deionized water constant volume, when constant volume, by dripping pH=6 ~ 7 of hydrochloric acid control A liquid;
Step 2) configuration B liquid: the polyvinylpyrrolidone adding the TMB of 1.2g, the absolute ethyl alcohol of 80g, the oxine of 2.6g, the hydrogen phosphide cumene of 2g and 4g in the B liquid of every 1000mL respectively, use chloroform constant volume;
Step 3) filter paper is immersed in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 60 DEG C, drying time is 5 minutes;
Step 4) filter paper is immersed in B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 40 DEG C, drying time is 3 minutes;
Step 5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make the reagent strip detecting creatinine content in urine.Substrate material can be Corvic, polypropylene or polymethylmethacrylate.
Embodiment 2
The reagent strip of creatinine content in one detection urine, comprises substrate and filter paper, prepares as follows:
Step 1) configuration A liquid: the polyglycol adding the β-phosphoglycerol disodium of 250g, the sodium citrate of 3.5g, the copper sulphate of 2.0g and 0.5g in the A liquid of every 1000mL respectively, use deionized water constant volume, when constant volume, by dripping pH=6 ~ 7 of hydrochloric acid control A liquid;
Step 2) configuration B liquid: add the TMB of 1.8g, the absolute ethyl alcohol of 120g, the 2-hydroxyquinoline of 3.2g, the di-isopropylbenzene hydroperoxide of 3g and the polyglycol of 8g in the B liquid of every 1000mL respectively, use chloroform constant volume;
Step 3) filter paper is immersed in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 80 DEG C, drying time is 20 minutes;
Step 4) filter paper is immersed in B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 60 DEG C, drying time is 10 minutes;
Step 5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make the reagent strip detecting creatinine content in urine.
Embodiment 3
The reagent strip of creatinine content in one detection urine, comprises substrate and filter paper, prepares as follows:
Step 1) configuration A liquid: the polyglycol adding the β-phosphoglycerol disodium of 250g, the sodium citrate of 3.5g, the copper sulphate of 2.0g and 0.5g in the A liquid of every 1000mL respectively, use deionized water constant volume, when constant volume, by dripping pH=6 ~ 7 of hydrochloric acid control A liquid;
Step 2) configuration B liquid: add 3 of 1.8g respectively in the B liquid of every 1000mL, 3 ', 5, the absolute ethyl alcohol of 5 '-tetramethyl benzidine, 120g, the 8-methylquinoline of 3.2g, the tert-butyl hydroperoxide of 2.5g and the polyvinylpyrrolidone of 8g, use chloroform constant volume;
Step 3) filter paper is immersed in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 80 DEG C, drying time is 20 minutes;
Step 4) filter paper is immersed in B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 60 DEG C, drying time is 10 minutes;
Step 5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make the reagent strip detecting creatinine content in urine.
Comparative example 1
The reagent strip of creatinine content in one detection urine, comprises substrate and filter paper, prepares as follows:
Step 1) configuration A liquid: the polyvinylpyrrolidone adding the β-phosphoglycerol disodium of 200g, the sodium citrate of 2.5g, the copper sulphate of 1.2g and 0.1g in the A liquid of every 1000mL respectively, use deionized water constant volume, when constant volume, by dripping pH=6 ~ 7 of hydrochloric acid control A liquid;
Step 2) configuration B liquid: the polyvinylpyrrolidone adding the TMB of 1.2g, the absolute ethyl alcohol of 80g, the oxine of 2.6g and 4g in the B liquid of every 1000mL respectively, use chloroform constant volume;
Step 3) filter paper is immersed in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 60 DEG C, drying time is 5 minutes;
Step 4) filter paper is immersed in B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 40 DEG C, drying time is 3 minutes;
Step 5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make the reagent strip detecting creatinine content in urine.
Comparative example 2
The reagent strip of creatinine content in one detection urine, comprises substrate and filter paper, prepares as follows:
Step 1) configuration A liquid: the polyglycol adding the Tris damping fluid of 250g, the sodium citrate of 3.5g, the copper sulphate of 2.0g and 0.5g in the A liquid of every 1000mL respectively, use deionized water constant volume, when constant volume, by dripping pH=6 ~ 7 of hydrochloric acid control A liquid;
Step 2) configuration B liquid: the polyglycol adding the TMB of 1.8g, the absolute ethyl alcohol of 120g, the oxine of 3.2g, the hydrogen phosphide cumene of 3g and 8g in the B liquid of every 1000mL respectively, use chloroform constant volume;
Step 3) filter paper is immersed in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 80 DEG C, drying time is 20 minutes;
Step 4) filter paper is immersed in B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 60 DEG C, drying time is 10 minutes;
Step 5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make the reagent strip detecting creatinine content in urine.
Comparative example 3
The reagent strip of creatinine content in one detection urine, comprises substrate and filter paper, prepares as follows:
Step 1) configuration A liquid: the polyglycol adding the Tris damping fluid of 250g, the sodium citrate of 3.5g, the copper sulphate of 2.0g and 0.5g in the A liquid of every 1000mL respectively, use deionized water constant volume, when constant volume, by dripping pH=6 ~ 7 of hydrochloric acid control A liquid;
Step 2) configuration B liquid: the polyglycol adding the TMB of 1.8g, the absolute ethyl alcohol of 120g, the oxine of 3.2g and 8g in the B liquid of every 1000mL respectively, use chloroform constant volume;
Step 3) filter paper is immersed in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 80 DEG C, drying time is 20 minutes;
Step 4) filter paper is immersed in B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 60 DEG C, drying time is 10 minutes;
Step 5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make the reagent strip detecting creatinine content in urine.
Embodiment 4
The preparation method of standard color comparison card:
(1) the creatinine standard solution of 0mmol/L, 4.4mmol/L, 8.8mmo1/L, 17.6mmol/L, 26.4mmol/L is configured;
(2) be dipped in respectively in five kinds of above-mentioned creatinine standard solution by the detection reagent strip that above-described embodiment 1 ~ 3 prepares, take out, dry after 2 seconds, after 1 minute, colour developing completely;
(3) prepare colorimetric card according to the colour developing situation of each test paper, the color range of colorimetric card is colourless to navy blue 5 color ranges.
Embodiment 5
The using method of reagent strip:
The reagent strip that embodiment 1 ~ 3 prepares is immersed in liquid to be measured, take out after 2 seconds, dry, after 1 minute, colour developing completely, compare with standard color comparison card subsequently, be the concentration of liquid to be measured with the concentration corresponding to the color on the immediate standard color comparison card of color on reagent strip, if between two color ranges, then can estimate and read creatine concentration.
Embodiment 6
The using method of urine creatinine detection reagent strip on instrument:
(1) the creatinine standard solution of 0mmol/L, 4.4mmol/L, 8.8mmol/L, 17.6mmol/L, 26.4mmol/L is configured;
(2) reagent strip that embodiment 1 ~ 3 prepares is dipped in respectively in five kinds of above-mentioned creatinine standard solution;
(3) Urine Analyzer is used to measure the reflectivity of 1 minute, according to 5 point-rendering typical curves;
(4) when using, immersed in liquid to be measured by the reagent strip prepared take out according to embodiment, use Urine Analyzer to measure reflectivity after 1 minute, the concentration that the reflectivity recorded is corresponding with on typical curve is liquid creatine concentration to be measured.
Below embodiment 1 ~ 3 and the shelf-life at most under the colour developing intensity of variation of comparative example 1 ~ 3 in the creatinine titer of variable concentrations, develop the color complete spent developing time and storage at normal temperature:
| Creatine concentration | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| 4.4mmol/L | Micro-indigo plant | Micro-indigo plant | Micro-indigo plant | Do not develop the color | Blue | Micro-indigo plant |
| 8.8mmol/L | Light blue | Light blue | Light blue | Do not develop the color | Blue | Micro-prison |
| 17.6mmol/L | Blue | Blue | Blue | Do not develop the color | Blue | Micro-prison |
| 26.4mmol/L | Dark blue | Dark blue | Dark prison | Do not develop the color | Blue | Light blue |
| Developing time | 1min | 1min | 1min | - | 3min | 5min |
| Shelf-life | 1 year | 1 year | 1 year | - | 3 months | Half a year |
Although embodiment of the present invention are open as above, but it is not restricted to listed in instructions and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details.
Claims (10)
1. detect a reagent strip for creatinine content in urine, comprise substrate and filter paper, described filter paper attachment on the substrate, is immersed successively in described filter paper and is had A liquid and B liquid, wherein:
Described in every 1000mL, A liquid comprises: the surfactant of the β-phosphoglycerol disodium of 200 ~ 250g, the sodium citrate of 2.5 ~ 3.5g, the copper sulphate of 1.2 ~ 2.0g and 0.1 ~ 0.5g, and uses deionized water constant volume;
Described in every 1000mL, B liquid comprises: the surfactant of the absolute ethyl alcohol of 1.2 ~ 1.8g reductibility chromogen, 80 ~ 120g, the antioxidant of 2.6 ~ 3.2g, the superoxide of 2 ~ 3g and 4 ~ 8g, and uses chloroform constant volume.
2. the reagent strip of creatinine content in detection urine according to claim 1, is characterized in that, described antioxidant is selected from oxine, 2-hydroxyquinoline, 8-methylquinoline or its combination, described reductibility chromogen is 3,3 ', 5,5 '-tetramethyl benzidine.
3. the reagent strip of creatinine content in detection urine according to claim 1, is characterized in that, in described A liquid, drip hydrochloric acid, and make pH=6 ~ 7 of described A liquid.
4. the reagent strip of creatinine content in detection urine according to claim 1, it is characterized in that, described substrate material is Corvic, or polypropylene, or polymethylmethacrylate.
5. the reagent strip of creatinine content in detection urine according to claim 1, is characterized in that, described surfactant is selected from polyvinylpyrrolidone, polyglycol or its combination.
6. the reagent strip of creatinine content in detection urine according to claim 1, is characterized in that, described superoxide is selected from hydrogen phosphide cumene, di-isopropylbenzene hydroperoxide, tert-butyl hydroperoxide or its combination.
7., as a preparation method for the reagent strip of creatinine content in detection urine arbitrary in claim 1 ~ 6, it is characterized in that, comprise the steps:
Step 1) configuration A liquid: the surfactant adding the β-phosphoglycerol disodium of 200 ~ 250g, the sodium citrate of 2.5 ~ 3.5g, the copper sulphate of 1.2 ~ 2.0g and 0.1 ~ 0.5g in the A liquid of every 1000mL respectively, use deionized water constant volume;
Step 2) configuration B liquid: the surfactant adding 1.2 ~ 1.8g reductibility chromogen, the absolute ethyl alcohol of 80 ~ 120g, the antioxidant of 2.6 ~ 3.2g, the superoxide of 2 ~ 3g and 4 ~ 8g in the B liquid of every 1000mL respectively, use chloroform constant volume;
Step 3) filter paper is immersed in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 60 ~ 80 DEG C, drying time is 5 ~ 20 minutes;
Step 4) filter paper is immersed in B liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 40 ~ 60 DEG C, drying time is 3 ~ 10 minutes;
Step 5) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make the reagent strip detecting creatinine content in urine.
8. the preparation method of the reagent strip of creatinine content in detection urine according to claim 7, is characterized in that, be also included in step 1) in make pH=6 ~ 7 of described A liquid by dripping hydrochloric acid.
9. the preparation method of the reagent strip of creatinine content in detection urine according to claim 7, it is characterized in that, described antioxidant is selected from oxine, 2-hydroxyquinoline, 8-methylquinoline or its combination, described reductibility chromogen is 3,3 ', 5,5 '-tetramethyl benzidine, described superoxide is selected from hydrogen phosphide cumene, di-isopropylbenzene hydroperoxide, tert-butyl hydroperoxide or its combination.
10. the preparation method of the reagent strip of creatinine content in detection urine according to claim 7, is characterized in that, described surfactant is selected from polyvinylpyrrolidone, polyglycol or its combination.
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Cited By (9)
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| CN106596913A (en) * | 2017-01-18 | 2017-04-26 | 重庆普西医疗设备有限公司 | Preparation method of quantitative analysis test paper for urine |
| CN106645676A (en) * | 2017-01-18 | 2017-05-10 | 重庆普西医疗设备有限公司 | Multi-term urine analysis test paper and preparation method thereof |
| CN108152282A (en) * | 2017-12-14 | 2018-06-12 | 吉林省汇酉生物技术股份有限公司 | A kind of reagent for detecting urine creatinine and its application |
| CN108169222A (en) * | 2017-12-14 | 2018-06-15 | 吉林省汇酉生物技术股份有限公司 | A kind of reagent for detecting urine creatinine and its application |
| CN109298188A (en) * | 2018-11-13 | 2019-02-01 | 广东众尔健生物科技有限公司 | One heavy metal species and creatinine combined detection test paper and the preparation method and application thereof |
| CN110346554A (en) * | 2018-04-02 | 2019-10-18 | 洛阳普莱柯万泰生物技术有限公司 | A kind of double-antibody method enzyme immunochromatographytest test kit and preparation method and application |
| CN110954530A (en) * | 2019-11-27 | 2020-04-03 | 迪瑞医疗科技股份有限公司 | Dry chemical test paper for detecting urine creatinine and preparation method thereof |
| CN113376150A (en) * | 2021-06-10 | 2021-09-10 | 吉林基蛋生物科技有限公司 | Urine microalbumin dry chemical detection test paper and preparation method thereof |
| CN114034693A (en) * | 2021-11-04 | 2022-02-11 | 广州达安基因股份有限公司 | Combined test paper for detecting urine creatinine urine microalbumin semiquantitative, preparation method and application thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106596913A (en) * | 2017-01-18 | 2017-04-26 | 重庆普西医疗设备有限公司 | Preparation method of quantitative analysis test paper for urine |
| CN106645676A (en) * | 2017-01-18 | 2017-05-10 | 重庆普西医疗设备有限公司 | Multi-term urine analysis test paper and preparation method thereof |
| CN108152282A (en) * | 2017-12-14 | 2018-06-12 | 吉林省汇酉生物技术股份有限公司 | A kind of reagent for detecting urine creatinine and its application |
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| CN110346554A (en) * | 2018-04-02 | 2019-10-18 | 洛阳普莱柯万泰生物技术有限公司 | A kind of double-antibody method enzyme immunochromatographytest test kit and preparation method and application |
| CN109298188A (en) * | 2018-11-13 | 2019-02-01 | 广东众尔健生物科技有限公司 | One heavy metal species and creatinine combined detection test paper and the preparation method and application thereof |
| CN110954530A (en) * | 2019-11-27 | 2020-04-03 | 迪瑞医疗科技股份有限公司 | Dry chemical test paper for detecting urine creatinine and preparation method thereof |
| CN113376150A (en) * | 2021-06-10 | 2021-09-10 | 吉林基蛋生物科技有限公司 | Urine microalbumin dry chemical detection test paper and preparation method thereof |
| CN114034693A (en) * | 2021-11-04 | 2022-02-11 | 广州达安基因股份有限公司 | Combined test paper for detecting urine creatinine urine microalbumin semiquantitative, preparation method and application thereof |
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Application publication date: 20150429 |