CN105388146A - Kit for simultaneously detecting sodium, creatinine and microalbumin in urine - Google Patents
Kit for simultaneously detecting sodium, creatinine and microalbumin in urine Download PDFInfo
- Publication number
- CN105388146A CN105388146A CN201510683512.7A CN201510683512A CN105388146A CN 105388146 A CN105388146 A CN 105388146A CN 201510683512 A CN201510683512 A CN 201510683512A CN 105388146 A CN105388146 A CN 105388146A
- Authority
- CN
- China
- Prior art keywords
- creatinine
- sodium
- damping fluid
- microalbumin
- urine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6827—Total protein determination, e.g. albumin in urine
- G01N33/683—Total protein determination, e.g. albumin in urine involving metal ions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/70—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving creatine or creatinine
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Plasma & Fusion (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention provides a kit for simultaneously detecting sodium, creatinine and microalbumin in urine. The kit comprises a reaction substrate with a sodium reaction hole, a creatinine reaction hole and a microalbumin reaction hole, and sodium diluent, creatinine diluents and standard colorimetric plates for sodium, creatinine and albumin. Reaction mats containing different configuration substrates and enzyme solutions are added to the three reaction holes, and semiquantitative detection on sodium, creatinine and microalbumin in urine is achieved. The kit is simple in preparation method, stable in performance and easy to store, color gradations corresponding to the content of sodium, creatinine and microalbumin in different kinds of urine are obvious, whether the salt amount of a detected person exceeds the standard or not and whether the kidney is damaged or not can be conveniently judged, and meanwhile the kit is suitable for being used by the clinical laboratory in a hospital and non-professional inspection personnel.
Description
Technical field
The present invention relates to the method for sodium in enzymatic assays urine, creatinine and microalbumin content, and application the method formulated while detect the dry chemistry reagent box of urine sodium, creatinine and microalbumin, belong to medical test determination techniques field.
Background technology
The excessive absorption of sodium can cause Hypertensive disease, albuminuria, artery sclerosis etc., and the progress to end stage renal failure, play an important role.Not only can reduce blood pressure while reduction sodium is taken in, also can reduce the excretion of Urine proteins.Sodium has played central role in the distribution maintaining normal moisture and osmotic pressure.Kidney is the major organs regulating sodium and moisture, maintenance acid base equilibrium.Sodium freely can be leached by kidney, and wherein the overwhelming majority is heavily absorbed again.Urine sodium excretion also with diet containing sodium salt and body saturation state closely related.Abnormal results: reduce: see that adrenal cortex function is hyperfunction, Cushing syndrome, primary aldosteronism, congestive heart failure etc.In addition, vomiting, diarrhoea, gastrointestinal surgery fistulization, large-area burns etc. also can make renal sodium excretion reduce.Raise: see pipe necrosis (oliguria stage), diabetes insipidus, hypoadrenocorticism etc. in serious pyelonephritis, renal damage, diabetes, acute kidney.
The mensuration of current electrolysis matter sodium has chemical method, flame photometry and ion selective electrode method, with the development of modern analytical technique.Chemical method gradually by sensitive, fast instrumental method replace.Current most of hospital be flame photometry and ion selective electrode method, the latter again with its response fast (being suitable for emergency treatment), sensitive, result accurately and more and more by the favor of laboratory personnel.There is again electrolyte enzymatical detection method in recent years, and fully-automatic analyzer can have been reinstated with other conventional sense project one and detect, accurately facilitate, also reduce testing cost simultaneously.The domestic and international existing procucts listing of enzymatic assays sodium reagent box, mainly liquid double reagent, but Jin You minority producer can produce, and liquid reagent is very unstable.
Creatinine (creatinine, Cre) is the product of muscle human body metabolism, and every 20g muscle metabolism can produce 1mg creatinine.Creatinine excretes primarily of glomerular filtration.In blood, creatinine is from exogenous and endogenous two kinds, and exogenous creatinine is the product of meat food in vivo after metabolism; Endogenous creatinine is the product of musculature metabolism in body.When meat food intake is stablized.The muscle metabolism of health does not have again large change, and the generation of creatinine will be more constant.UCr mainly carrys out autoblood, excretes after glomerular filtration with urine.UCr inspection can measure the creatinine content that blood is discharged through glomerular filtration.UCr value normal range: 8.4 ~ 13.25mmol/24h urinates or 40 ~ 130mg/dl urine.The daily output quite stable of normal person's UCr, the impact of basic unable to take food thing protein content and urine volume.When creatinine content in urine significantly increases or reduces, then reflect that renal function is subject to damage to a certain extent.UCr excretion increases: see hypothyroidism, some deeline, liver disease, diabetes, acromegalia, gigantism, heating and hunger etc.UCr excretion reduces: see deeline and muscular atrophy and the muscular dystrophies etc. such as renal function is incomplete, hyperthyroidism, anaemia, paralysis, typhoid fever, lockjaw, tuberculosis.Therefore, check that the change of UCr content can play important reference role to the change of renal function and treatment.
Creatinine routine biochemistry detection method mainly contains two large classes, one is chemical method---alkaline picric acid end-point method and alkaline picric acid 2 KINETIC METHOD, because end-point method is too many by the pseudo-kind that is positive and the negative chaff interference of puppet of serum endogenous, what have in these chaff interferences reacts also faster than creatinine with picric acid, be called " fast chaff interference ", as acetic acid, acetoacetate; Some ratio creatinine reactions are slow, claim " slow chaff interference ", as glucose, some residue of protein etc.Except pseudo-positive interference, also there is pseudo-negative chaff interference, commonly cholerythrin.Cholerythrin can make creatinine assay Lower result, and when bilirubin concentration rises to 35mg/dl (600 μm of ol/L), interference obviously.Now have nearly half clinical chemistry laboratory in national Grade III Class A hospital creatinine enzyme process is applied in blood/UCr conventional sense.In the development of clinical chemistry methodology, the utilization of Enzymology method, makes clinical chemistry determination techniques have a qualitative leap.Its core is that enzyme action specificity is high, and the accuracy of mensuration, sensitivity, repeatability, linear measurement range etc. have been had and increases substantially accordingly, the universal employing strong acid that makes of enzyme process, the chemical method of highly basic is gradually eliminated.Enzymatic reaction condition is gentle, along with the development of commercially available reagent box, then with semi-automatic or automatic clinical chemistry analyzer is supporting, makes detection quick, simple and easy, efficiently.The creatinine enzyme process measuring principle that developed recently gets up, all has commercially available reagent box supply, wherein has single reagent also to have different dosage form and the specification of double reagent.Enzyme process improves the specificity of detection, but is not absolute specificity.Therefore, though belong to together enzyme process because of concrete measuring principle is different and same measuring principle and select the source of toolenzyme, purity, specific activity, particularly toolenzyme action specificity difference also there is interference dissimilar and not of uniform size.Creatinine enzymatic analysis has had in recent years and has developed rapidly, the domestic and international existing procucts listing of enzymatic assays creatinine reagent box, mainly liquid double reagent, but Jin You minority producer can produce, and liquid reagent is very unstable.
Microdose urine protein is the index of reflection detection of glomeruli filtration function, indicate early stage kidney damage, also reflect human body vascular endothelial dysfunction, the function status of blood vessel can be reflected by the microalbumin detected in urine, if early treatment renal function still may recover.But as not early treatment, these patients develop into High-grade Proteinuria from microalbuminuria, the renal function of indication patient worsens and irreversible.Therefore by detecting microalbuminuria prediction early nephropathy and vascular function, carrying out early intervention treatment, for improving the kidney damage that hypertension or diabetes cause, there is important clinical meaning.
Albumin detection method has colloidal gold method, immunoturbidimetry, the affine chemical method of dyestuff etc., current Related product has launch all at home and abroad, colloidal gold method needs professional to operate, complex manufacturing, and immunoturbidimetry testing cost is high, need necessary instrument, the simplest method is the affine method of dyestuff, albumin in urine and sulfonaphthaleins dyestuff react and form blue product, be directly proportional to the albumin concentration in urine in color depth, only by dyestuff solid phase in chromatographic film, interpretation need be carried out by instrument or naked eyes.
To sum up, predict water-sodium retention, glomerulus excretion situation and the absorption situation of sodium salt by the excretion detecting urine sodium, to nephrogenic hypertension particularly volume dependent type hypertension have good predicting function; Predict whether kidney has damage by the excretion detecting microdose urine protein, whether renal blood vessels pathology occurs changes the function that kidney filters protein (especially albumin); Microalbuminuria is also the Symptoms at Primary Stage that kidney and cardiovascular system change.Prevention nephrogenic hypertension is played an important role.Also do not have to detect the dry chemistry reagent box of sodium in urine, creatinine and microalbumin or the application of similar approach at present simultaneously, not enough problem is equipped with, in the urgent need to providing the half-quantitative detection kit of sodium, creatinine and microalbumin in a kind of quick, easy, half-quantitative detection urine in order to adapt to needs that testing agency of basic unit detects the non-quantitation of sodium, creatinine and microalbumin in urine and detect unit detection hardware.
Summary of the invention
The object of this invention is to provide a kind of dry chemistry reagent box simultaneously detecting urine sodium, creatinine and microalbumin.
A kind of kit simultaneously detecting sodium, creatinine and microalbumin in urine provided by the invention, comprising: (1) has the reaction substrate of sodium reacting hole, creatinine reacting hole and microalbumin reacting hole;
(2) sodium dilution, creatinine dilution;
(3) there is the standard colorimetric plate of variable concentrations sodion standard coloration, variable concentrations creatinine standard coloration and variable concentrations microalbumin standard coloration.
Described sodium reacting hole is placed with substrate pad and enzyme pad successively from bottom hole;
Described substrate pad is dripped by substrate solution and is prepared from carrier; Substrate solution with the damping fluid of 100-1000mmol/LpH7.5-9.0 for solvent, the beta galactosidase substrate containing 0.001-0.01g/mL, the cave ether of 20-80mmol/L, the stabilizing agent of 0.005%-50% and the surfactant of 0.005%-50%;
Described enzyme pad is dripped by enzyme solutions and is prepared from carrier; Enzyme solutions with the damping fluid of 100-1000mmol/LpH7.0-9.0 for solvent, the beta galactosidase containing 200-3000U/mL and 0.01-100g/L enzyme stabilizers.
Preferably, described enzyme solutions with the damping fluid of 100-1000mmol/LpH7.0-9.0 for solvent, the beta galactosidase containing 500-1500U/mL and 10-20g/L stabilizing agent.
Above-mentioned damping fluid all can be selected from one or more of kaliumphosphate buffer, Tris-HCl damping fluid or PIPES damping fluid.
Preferably, the damping fluid of substrate solution is the Tris-HCl damping fluid of 800-1000mmol/LpH8.0-9.0; The damping fluid of enzyme solutions is the Tris-HCl damping fluid of 100-200mmol/LpH7.0-8.0.
The beta galactosidase substrate contained in substrate pad is O-nitro-β-D-pyranoside (ONPG), the chloro-3-indoles of the bromo-4-of 5--β-D-galactopyranoside (x-gal), chlorophenol red-β-D-galactopyranoside (CPRG), PNPG, IPTG.
Preferably, beta galactosidase substrate is the bromo-4-of 5-chloro-3-indoles-β-D-galactopyranoside (x-gal) and chlorophenol red-β-D-galactopyranoside (CPRG).
The cave ether contained in substrate pad is one or more in 4,7,13,16,21-five oxa--1,10-diazabicyclo [8.8.5] tricosane, 18-Crown-8 or 15-Crown-5.Preferably, described cave ether is 4,7,13,16,21-five oxa--1,10-diazabicyclo [8.8.5] tricosane (Kryptofix)
The stabilizing agent contained in substrate pad is one or more in D-trehalose, sucrose, Dextran T 70, beta-schardinger dextrin-, bovine serum albumin(BSA), sweet mellow wine, alpha-lactose, polyglycol, gelatin, inositol, wood sugar or arabite, and preferred stabilizer is sucrose and D-trehalose;
The surfactant contained in substrate pad is one or more in Tween 80, polysorbas20, TritonX-100, NP40 or sorbierite, and preferred surfactant is Tween 80.
The stabilizing agent contained in enzyme pad is one or more in D-trehalose, sucrose, Dextran T 70, beta-schardinger dextrin-, bovine serum albumin(BSA), sweet mellow wine, polyglycol, gelatin, inositol, wood sugar, arabite, alpha-lactose, maleic acid, tartrate or citric acid, and preferred stabilizer is alpha-lactose and Dextran T 70.
Creatinine reacting hole in reaction substrate in a kind of kit simultaneously detecting sodium, creatinine and microalbumin in urine provided by the invention is placed with underlay and upper pad successively from bottom hole;
Described underlay is dripped by underlay solution and is prepared from carrier; Underlay solution take water as solvent, the peroxidase containing 10-2000U/mL, 10-1000U/mL creatinine amidohydrolase hydrolytic enzyme, the damping fluid of the developer 1,5-500mmol/LpH6.0-8.0 of 0.1%-10%, the stabilizing agent of 0.005%-50%;
Preferably, in underlay solution, peroxidase activity is 800-1500U/mL, and creatinine amidohydrolase hydrolysis activity is 500-1000U/mL;
Described developer 1 is one or more in the amino antipyrine of 4-, 3-methyl-2-benzothiazolinone hydrazone hydrochloride hydrate (MBTH); Preferred described developer 1 is the amino antipyrine of 4-of 0.5%-2%;
The damping fluid of described 5-500mmol/LpH6.0-8.0 is one or more of sodium phosphate buffer, kaliumphosphate buffer, Tris-HCl damping fluid or PIPES damping fluid, and preferred described damping fluid is the kaliumphosphate buffer of 50-200mmol/LpH7.0-8.0;
The stabilizing agent of described 0.005%-50% is one or more in D-trehalose, sucrose, Dextran T 70, beta-schardinger dextrin-, bovine serum albumin(BSA), sweet mellow wine, polyglycol, gelatin, inositol, wood sugar, arabite, alpha-lactose, maleic acid, tartrate or citric acid, and preferred described stabilizing agent is 1%-20% sucrose and inositol.
The upper pad of the creatinine reacting hole in the reaction substrate in the kit simultaneously detecting sodium, creatinine and microalbumin in urine of the present invention is dripped by upper pad solution and is prepared from carrier; Upper pad solution take water as solvent, the creatine amidino groups hydrolytic enzyme of the sarcosine oxidase containing 10-2000U/mL, 10-2000U/mL, the damping fluid of the developer 2,5-500mmol/LpH6.0-8.0 of 0.1%-10%, the stabilizing agent of 0.005%-50%;
Preferably, in upper pad solution, sarcosine oxidase vigor is 10-500U/mL, and creatine amidino groups hydrolysis activity is 20-500U/mL.
Described developer 2 is 2,4, the bromo-3-hydroxybenzoic acid (TBHB) of 6-tri-, 3, one or more in the chloro-2-hydroxy benzene sulfonic acid (DCHBS) of 5-bis-, N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3-methylaniline (TOOS), N-ethyl-N-(3-sulfopropyl)-3-methylaniline (TOPS), two (4-sulphur the butyl)-3-methylaniline disodium salt of N, N-.Preferred developer 2 is one or more in TBHB, DCHBS of 1%-10%.
The damping fluid of described 5-500mmol/LpH6.0-8.0 is one or more of sodium phosphate buffer, kaliumphosphate buffer, Tris-HCl damping fluid or PIPES damping fluid, and preferred buffer is the kaliumphosphate buffer of 50-200mmol/LpH7.0-8.0;
The stabilizing agent of described 0.005%-50% is one or more in D-trehalose, sucrose, Dextran T 70, beta-schardinger dextrin-, bovine serum albumin(BSA), sweet mellow wine, polyglycol, gelatin, inositol, wood sugar, arabite, alpha-lactose, maleic acid, tartrate or citric acid, and preferred stabilizer is 1%-20% sucrose and Dextran T 70.
Colour developing pad is had in microalbumin reacting hole in reaction substrate in a kind of kit simultaneously detecting sodium, creatinine and microalbumin in urine provided by the invention; Described colour developing pad is dripped by developing dye solution and is prepared from carrier; Described developing dye solution take water as the spreading agent of solvent, the dyestuff containing 0.02-20mg/mL, the damping fluid of 0.1-2mol/LpH1.5-3.0,0.1-2%;
Described dyestuff is selected from 5 ' 5 "-2-nitro-3 ', 3 "-two iodo-3,4,5,6-tetrabromophenol sulfonphthaleins (DIDNTB), bromophenol blue, one or more in Tetrabromophenol Blue; Preferred coloring agent is the DIDNTB of 1-5mg/mL.
Described damping fluid is one or more of sodium phosphate buffer, kaliumphosphate buffer, citric acid solution, Tris-HCl damping fluid or PIPES damping fluid; Preferred buffer is the citrate buffer solution of 1.5-2mol/LpH1.5-3.0.
Described spreading agent is selected from one or more in polyvinyl alcohol (PVA), triethyl hexyl phosphoric acid, lauryl sodium sulfate, methyl amyl alcohol, cellulose derivative, polyacrylamide, guar gum.Preferred described spreading agent is the polyvinyl alcohol (PVA) of 0.5-1.5%.
The carrier that sodium reacting hole, creatinine reacting hole and microalbumin reacting hole in reaction substrate in a kind of kit simultaneously detecting sodium, creatinine and microalbumin in urine of the present invention adopt is filter paper, glass fibre or chromatographic paper.
The size of the sodium reacting hole of kit of the present invention, creatinine reacting hole and microalbumin reacting hole can be
preferably
Provided by the inventionly a kind ofly detect in the kit of sodium, creatinine and microalbumin in urine, described sodium dilution is one or more of the sodium phosphate buffer of the 0.1-1.5mol/L of pH8.0-9.0, kaliumphosphate buffer, citric acid solution, Tris-HCl damping fluid or PIPES damping fluid simultaneously; Preferred described damping fluid is the Tris-HCl damping fluid of the 0.5-1.2mol/L of pH8.0-8.8.
Described creatinine dilution is one or more of the sodium phosphate buffer of the 0.1-1.2mol/L of pH6.5-8.0, kaliumphosphate buffer, citric acid solution, Tris-HCl damping fluid or PIPES damping fluid.Preferred described damping fluid is the kaliumphosphate buffer of the 0.5-1.2mol/L of pH7.0-8.0.
The invention provides mentioned reagent box and detect application in sodium content in urine, creatinine content and microalbumin content at the same time.
It will be appreciated by those skilled in the art that in this area, detecting sodium content in urine is sodium ions content in detection urine.Consider the needs that kit is preserved, prepared reacting pad must possess quite high stability, inventor is on the basis of lot of experiments, determine composition and the formula thereof of above-mentioned enzyme liquid, nitrite ion and various substrate solution, make kit have desirable stability, can promote in actual applications.
In the present invention, sodion and creatinine index are used for weighing the change that sodium is taken in prediction for 24 hours, and creatinine and microalbumin index are used for weighing kidney damage, are the auxiliary diagnosises of hypertension, cardiovascular patient hazards.Based on these results of study, the present invention establishes a kind of dry chemical that is easy, that simultaneously detect urine sodium, creatinine and microalbumin fast and measures kit.
Adopt technique scheme, the invention has the advantages that: the instability problem that the invention solves liquid reagent, by adding the material such as activator, enzyme stabilizers, on both guarantee reagent pads stable reagent prerequisite under, sensitivity and the accuracy of reaction can be improved again fast; Kit is fast easy and simple to handle, and accuracy is high, is applicable to the routine clinical detection particularly other detection use of clinical patient bed, and then instructs sound clinical medication.Only the urine of collection need be diluted, be added in corresponding reacting hole, reaction a period of time carries out colorimetric, measure tested urine place sodium value (creatinine and Microalbunin white value) interval range, assessment tester take in sodium number, do not need large-scale instrument and equipment, easy and simple to handle, can use at hospital emergency rooms, primary care and care facilities and patient family.Kit of the present invention avoids the one-sidedness and disturbing factor of observing separately the generation of single index; And relative, collect single urine and there is the advantages such as simple, quick.
Embodiment
Following examples further illustrate content of the present invention, but should not be construed as limitation of the present invention.Without departing from the spirit and substance of the case in the present invention, the amendment do the inventive method, step or condition or replacement, all belong to scope of the present invention.
If do not specialize, the conventional means that technological means used in embodiment is well known to those skilled in the art.
Embodiment 1 detects the preparation of the dry chemistry reagent box of urine sodium, creatinine and microalbumin simultaneously
The kit detecting urine sodium, creatinine and microalbumin while the present embodiment comprises: the reaction substrate with sodium reacting hole, creatinine reacting hole and microalbumin reacting hole; Sodium dilution, creatinine dilution; There is the standard colorimetric plate of variable concentrations sodion standard coloration, variable concentrations creatinine standard coloration and variable concentrations microalbumin standard coloration.
The preparation method of the above-mentioned urine of detection simultaneously sodium, creatinine and microalbumin kit is as follows:
(1) structure of substrate is reacted:
The reaction substrate of the present embodiment is selected from ABS engineering plastics and makes, and which is provided with sodium reacting hole, creatinine reacting hole and microalbumin reacting hole; Reacting hole is of a size of
(2) preparation of substrate pad in sodium reacting hole:
Sodium reacting hole is placed with substrate pad and enzyme pad successively from bottom hole.
Substrate pad is dripped by substrate solution 1 μ L
on chromatographic paper, under lucifuge condition, 25 DEG C of moving airs are obtained after dry 4 hours, and sealing is kept at 4 DEG C.
Substrate solution with the Tris-HCl damping fluid of 1000mmol/LpH8.7 for solvent, the cave ether [4 of beta galactosidase substrate CPRG, 50mmol/L containing 0.005g/mL, 7,13,16,21-five oxa--1,10-diazabicyclo [8.8.5] tricosane (Kryptofix)], the D-trehalose of 10% and the Tween 80 of 0.05%.
Enzyme pad is dripped by enzyme solutions 1 μ L
on chromatographic paper, under lucifuge condition, 25 DEG C of moving airs are prepared from after dry 4 hours, and sealing is kept at 4 DEG C.
Enzyme solutions with the Tris-HCl damping fluid of 100mmol/LpH7.5 for solvent, the beta galactosidase containing 800U/mL and 10g/L enzyme stabilizers
(3) preparation of underlay and upper pad in creatinine reacting hole:
Creatinine reacting hole is placed with underlay and upper pad successively from bottom hole.
The preparation of underlay: 1 μ L underlay solution is dripped
on chromatographic paper, under lucifuge condition, 25 DEG C of moving airs are after dry 4 hours, and sealing is kept at 4 DEG C.
Underlay solution take water as solvent, the peroxidase containing 1000U/mL, 800U/mL creatinine amidohydrolase hydrolytic enzyme, the amino antipyrine of 4-of 1%, the kaliumphosphate buffer of 100mmol/LpH7.3, the sucrose of 10%.
The preparation of upper pad: drip 1 μ L padding solution
on chromatographic paper, under lucifuge condition, 25 DEG C of moving airs are after dry 4 hours, and sealing is kept at 4 DEG C.
Upper pad solution take water as solvent, the creatine amidino groups hydrolytic enzyme of the sarcosine oxidase containing 300U/mL, 200U/mL, the kaliumphosphate buffer of the TBHB of 1%, 100mmol/LpH7.3, the sucrose of 10%.
(4) develop the color in microalbumin reacting hole the preparation of padding: colour developing pad is dripped by 1 μ L developing dye solution and exists
on chromatographic paper, under lucifuge condition, 25 DEG C of moving airs are after dry 4 hours, and sealing is kept at 4 DEG C.
Described developing dye solution take water as solvent, the dyestuff 5 ' 5 containing 2mg/mL "-2-nitro-3 ', 3 "-two iodo-3,4,5,6-tetrabromophenol sulfonphthaleins (DIDNTB), the citrate buffer solution of 1mol/LpH2.5, the polyvinyl alcohol (PVA) of 1%.
(5) each reacting pad prepared by step (2) ~ (4) is inserted in corresponding reacting hole, use stamping machine compacting, then stick aluminium foil joint strip, load in aluminium foil bag, the reaction substrate of sodium reacting hole, creatinine reacting hole and microalbumin reacting hole must be had.
(6) the Tris-HCl buffer solution 1000mL of the preparation of sodium dilution: 0.8mol/LpH8.7, fully after mixing, is divided in drop bottle and preserves.
The buffer solution of potassium phosphate 1000mL of the preparation of creatinine dilution: 1mol/LpH7.3, fully after mixing, is divided in drop bottle and preserves.
(7) preparation of standard colorimetric plate: preparation sodium, creatinine and microalbumin titer, concrete compound method is as follows:
1) urine is collected in basic urine preparation: collect in three days and do not take in high natriuresis liquid 5 parts (urina sanguinis 8:00-9:00 is best), adopt this product to measure na concn in urine to specifications and answer (answering <20mmol/L), creatine concentration (answering <0.15g/L), albumin concentration (answering <30mg/L), abundant mixing, through 0.45 μm of film suction filtration, based on urine, 2 DEG C ~ 8 DEG C preservations.Basic urine can use as " negative sample " simultaneously.
2) preparation of titer
A the preparation of () sodium chloride titer takes 0.585g sodium chloride and joins in 2ml purified water and dissolve, make 5000mmol/L sodium chloride titer.
B the preparation of () creatinine titer takes 0.1g creatinine and joins in 2ml purified water and dissolve, make 50g/L creatinine titer.
C the preparation of () albumin standards takes 10mg human serum albumins and joins in 2ml purified water and dissolve, make 5000mg/L human serum albumins titer.
3) preparation of each colour code sample
The preparation of the sample of (a) sodium Standard colour board: measure basic urine 4,20,40 μ l successively and put into three centrifuge tubes, be sequentially added into 996,980,960 μ l5000mmol/L sodium chloride titers, mixing, sealing is kept at 2 DEG C ~ 8 DEG C, use in 24 hours, final concentration sees the following form 1.
The preparation of the sample of (b) creatinine Standard colour board: measure basic urine 3,10,20,40 μ l successively and put into four centrifuge tubes, be sequentially added into 997,990,980,960 μ l50g/L creatinine titers, mixing, sealing is kept at 2 DEG C ~ 8 DEG C, use in 24 hours, final concentration sees the following form 1.
The preparation of the sample of (c) human albumin Standard colour board: measure basic urine 6,16,32,60 μ l successively and put into four centrifuge tubes, be sequentially added into 994,984,968,940 μ l5000mg/L human serum albumins titers, mixing, sealing is kept at 2 DEG C ~ 8 DEG C, use in 24 hours, final concentration sees the following form 1.
Table 1 each colour code sample concentration table
| Project | Colour code 1 | Colour code 2 | Colour code 3 | Colour code 4 |
| Sodium | 20mmol/L | 100mmol/L | 200mmol/L | —— |
| Creatinine | 0.15g/L | 0.5g/L | 1g/L | 2g/L |
| Microalbumin | 30mg/L | 80mg/L | 160mg/L | 300mg/L |
Be added drop-wise on reacting hole by the titer of above-mentioned different colour code and develop the color, estimated by 3-5 people, and compare with standard colorimetric plate, select standard colors, print, and verify, through repeatedly several times, finally settle the standard color board.
Embodiment 2 application that simultaneously can detect the dry chemistry reagent box of urine sodium, creatinine and microalbumin of the present invention
Kit of the present invention is the dry chemistry reagent box of a kind of half-quantitative detection urine sodium, creatinine and microalbumin, can detect the urine specimen of hypertensive patient clinically, also can detect the urine specimen of healthy population, to check whether the salt intake of crowd to be measured exceeds standard, can be used as the auxiliary characteristics of the diagnosis of hypertensive patient, antidiastole and therapeutic evaluation, also can be used as the aid that healthy population diet controls salt intake.
The concrete using method of kit prepared by the embodiment of the present invention 1 is as follows:
1) in use, first kit is taken out from storage temperature, equilibrate to room temperature and bring into use;
2) tear In Aluminium Foil Packing, take out reaction substrate;
3) urine process: get 25 μ l urines and add sodium, creatinine dilution respectively: fully mix; The volume ratio of dilution and urine is 1:20-35 (the present embodiment selects 1:30).
4) the urine 25 μ l after above-mentioned dilution is drawn respectively at sodium reacting hole, creatinine reacting hole; Microalbumin directly measures.
5) on dry bath (set temperature is 48 DEG C), react 7min, reaction result and color board are compared, sxemiquantitative can obtain measured object concentration.It will be appreciated by those skilled in the art that according to the state of the art, using under the condition that the set temperature of other cool-bags as incubator, baking oven, water bath containers etc. is 35-50 DEG C after reaction, the contrast that also can realize result judges.
Adopt kit of the present invention to clinically to salinity <6 gram every day, 90 volunteers (the salinity group that every 30 correspondences are different) of 6 ~ 12 grams and >12 gram detect, testing result rate of accuracy reached 100%.
Kit stable performance of the present invention, the shelf-life is 18 months.
Adopt kit of the present invention to detect urine sodium, creatinine and microalbumin clinically, compare with commercial urine sodium, creatinine and microalbumin quantification kit, kit testing result rate of accuracy reached more than 95% of the present invention.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1. detect a kit for sodium, creatinine and microalbumin in urine simultaneously, it is characterized in that, comprising:
(1) there is the reaction substrate of sodium reacting hole, creatinine reacting hole and microalbumin reacting hole;
(2) sodium dilution, creatinine dilution;
(3) there is the standard colorimetric plate of variable concentrations sodion standard coloration, variable concentrations creatinine standard coloration and variable concentrations microalbumin standard coloration.
2. kit according to claim 1, it is characterized in that, described sodium reacting hole is placed with substrate pad and enzyme pad successively from bottom hole;
Described substrate pad is dripped by substrate solution and is prepared from carrier; Substrate solution with the damping fluid of 100-1000mmol/LpH7.5-9.0 for solvent, the beta galactosidase substrate containing 0.001-0.01g/mL, the cave ether of 20-80mmol/L, the stabilizing agent of 0.005%-50% and the surfactant of 0.005%-50%;
Described enzyme pad is dripped by enzyme solutions and is prepared from carrier; Enzyme solutions with the damping fluid of 100-1000mmol/LpH7.0-9.0 for solvent, the beta galactosidase containing 200-3000U/mL and 0.01-100g/L enzyme stabilizers.
3. kit according to claim 2, is characterized in that, damping fluid is selected from one or more of kaliumphosphate buffer, Tris-HCl damping fluid or PIPES damping fluid.
4. kit according to claim 3, it is characterized in that, the damping fluid of substrate solution is the Tris-HCl damping fluid of 800-1000mmol/LpH8.0-9.0; The damping fluid of enzyme solutions is the Tris-HCl damping fluid of 100-200mmol/LpH7.0-8.0.
5. kit according to claim 2, it is characterized in that, described beta galactosidase substrate is O-nitro-β-D-pyranoside, the chloro-3-indoles of the bromo-4-of 5--β-D-galactopyranoside, chlorophenol red-β-D-galactopyranoside, PNPG, IPTG.
6. kit according to claim 2, it is characterized in that, described cave ether is one or more in 4,7,13,16,21-five oxa--1,10-diazabicyclo [8.8.5] tricosane, 18-Crown-8 or 15-Crown-5.
7. kit according to claim 1, it is characterized in that, described creatinine reacting hole is placed with underlay and upper pad successively from bottom hole;
Described underlay is dripped by underlay solution and is prepared from carrier; Underlay solution take water as solvent, the peroxidase containing 10-2000U/mL, 10-1000U/mL creatinine amidohydrolase hydrolytic enzyme, the damping fluid of the developer 1,5-500mmol/LpH6.0-8.0 of 0.1%-10%, the stabilizing agent of 0.005%-50%;
Described developer 1 is one or more in the amino antipyrine of 4-, 3-methyl-2-benzothiazolinone hydrazone hydrochloride hydrate;
Described upper pad is dripped by upper pad solution and is prepared from carrier; Upper pad solution take water as solvent, the creatine amidino groups hydrolytic enzyme of the sarcosine oxidase containing 10-2000U/mL, 10-2000U/mL, the damping fluid of the developer 2,5-500mmol/LpH6.0-8.0 of 0.1%-10%, the stabilizing agent of 0.005%-50%;
Described developer 2 is 2,4, the bromo-3-hydroxybenzoic acid of 6-tri-, 3, one or more in the chloro-2-hydroxy benzene sulfonic acid of 5-bis-, N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3-methylaniline, N-ethyl-N-(3-sulfopropyl)-3-methylaniline, two (4-sulphur the butyl)-3-methylaniline disodium salt of N, N-.
8. kit according to claim 1, is characterized in that, described microalbumin reacting hole has colour developing pad; Described colour developing pad is dripped by developing dye solution and is prepared from carrier; Described developing dye solution take water as the spreading agent of solvent, the dyestuff containing 0.02-20mg/mL, the damping fluid of 0.1-2mol/LpH1.5-3.0,0.1-2%;
Described dyestuff is selected from 5 ' 5 "-2-nitro-3 ', 3 "-two iodo-3,4,5,6-tetrabromophenol sulfonphthaleins, bromophenol blue, one or more in Tetrabromophenol Blue;
Described damping fluid is one or more of sodium phosphate buffer, kaliumphosphate buffer, citric acid solution, Tris-HCl damping fluid or PIPES damping fluid;
Described spreading agent is selected from one or more in polyvinyl alcohol (PVA), triethyl hexyl phosphoric acid, lauryl sodium sulfate, methyl amyl alcohol, cellulose derivative, polyacrylamide, guar gum.
9., according to the arbitrary described kit of claim 1-8, it is characterized in that, described carrier is filter paper, glass fibre or chromatographic paper.
10. the arbitrary described kit of claim 1-9 is detecting the application in urine in sodium content, creatinine content and microalbumin content.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510683512.7A CN105388146B (en) | 2015-10-20 | 2015-10-20 | Kit that is a kind of while detecting sodium in urine, creatinine and microalbumin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510683512.7A CN105388146B (en) | 2015-10-20 | 2015-10-20 | Kit that is a kind of while detecting sodium in urine, creatinine and microalbumin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105388146A true CN105388146A (en) | 2016-03-09 |
| CN105388146B CN105388146B (en) | 2018-08-17 |
Family
ID=55420676
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510683512.7A Active CN105388146B (en) | 2015-10-20 | 2015-10-20 | Kit that is a kind of while detecting sodium in urine, creatinine and microalbumin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105388146B (en) |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106353511A (en) * | 2016-08-19 | 2017-01-25 | 基蛋生物科技股份有限公司 | Urinary microalbumin (U-mALb)/urinary creatinine (U-Cr) integrated assay bigeminy strip and preparation method thereof |
| CN106645132A (en) * | 2017-02-09 | 2017-05-10 | 厦门信道生物技术有限公司 | Test paper for detecting lactose content of sample and preparation method of test paper |
| CN106645758A (en) * | 2017-01-03 | 2017-05-10 | 长沙中生众捷生物技术有限公司 | Detection reagent and test strip for creatinine |
| CN107358033A (en) * | 2017-06-26 | 2017-11-17 | 广东省人民医院(广东省医学科学院) | Appraisal procedure for the intake of Patients with Chronic Kidney Disease dietary protein |
| CN108120714A (en) * | 2017-12-20 | 2018-06-05 | 吉林省汇酉生物技术股份有限公司 | A kind of reagent for detecting urine microdose urine protein and its application |
| CN109799331A (en) * | 2019-03-18 | 2019-05-24 | 湖南海源医疗科技股份有限公司 | A kind of Tumor specific factor TSGF analysis strip |
| CN109856131A (en) * | 2019-01-22 | 2019-06-07 | 杭州联晟生物科技有限公司 | It is a kind of for detecting the three-in-one test card and preparation method thereof of uric acid in sample, creatinine, urea simultaneously |
| CN110297081A (en) * | 2018-03-23 | 2019-10-01 | 保生国际生医股份有限公司 | Detection method and its system |
| CN110716047A (en) * | 2019-10-24 | 2020-01-21 | 杭州康知生物科技有限公司 | Urine detection kit matched with kidney disease early-screening small-sized full-automatic analyzer |
| CN111579797A (en) * | 2020-05-27 | 2020-08-25 | 艾康生物技术(杭州)有限公司 | Detection test paper and application thereof |
| CN112577940A (en) * | 2019-09-30 | 2021-03-30 | 厦门大学 | Method for rapidly and quantitatively detecting concentration of creatinine in urine at low cost |
| CN113238060A (en) * | 2021-05-08 | 2021-08-10 | 迈克生物股份有限公司 | Kit for predicting or diagnosing myocarditis |
| CN113376383A (en) * | 2021-06-10 | 2021-09-10 | 吉林基蛋生物科技有限公司 | Preparation method of Vc interference resistant urine creatinine test paper |
| CN113418916A (en) * | 2021-07-02 | 2021-09-21 | 安徽惠邦生物工程有限公司 | Creatinine quantitative rapid detection test strip and preparation method thereof |
| CN115201134A (en) * | 2022-09-15 | 2022-10-18 | 吉林大学第一医院 | Creatinine detection kit resistant to piceatannol interference and application thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007292718A (en) * | 2006-03-28 | 2007-11-08 | Eiken Chem Co Ltd | Multi-item test paper and method for detecting test material in specimen |
| CN101865911A (en) * | 2010-03-16 | 2010-10-20 | 苏州市玮琪生物科技有限公司 | Urine creatine quantitative test card |
| JP2011080878A (en) * | 2009-10-07 | 2011-04-21 | Techno Medica Co Ltd | Sensor for measuring concentration of substance in urine and measuring device using the same |
| CN102757893A (en) * | 2011-04-28 | 2012-10-31 | 爱科来株式会社 | Dry test strip and method for measuring creatinine |
| CN103630535A (en) * | 2013-12-03 | 2014-03-12 | 上海科华生物工程股份有限公司 | Dry chemical test paper for quantitatively determining content of human blood albumin |
| CN103901034A (en) * | 2014-04-29 | 2014-07-02 | 中国科学院苏州生物医学工程技术研究所 | Detection reagent strip for detecting microalbumin in urine and preparation method of detection reagent strip |
| CN104198408A (en) * | 2014-08-14 | 2014-12-10 | 上海睿康生物科技有限公司 | Detection kit for determining content of creatinine in serum by enzymic method |
| CN104849270A (en) * | 2015-05-22 | 2015-08-19 | 汇征联合（北京）医疗器械有限公司 | Reagent for determining sodium chloride content in urine, test paper strip thereof, preparation method thereof and purpose thereof |
-
2015
- 2015-10-20 CN CN201510683512.7A patent/CN105388146B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007292718A (en) * | 2006-03-28 | 2007-11-08 | Eiken Chem Co Ltd | Multi-item test paper and method for detecting test material in specimen |
| JP2011080878A (en) * | 2009-10-07 | 2011-04-21 | Techno Medica Co Ltd | Sensor for measuring concentration of substance in urine and measuring device using the same |
| CN101865911A (en) * | 2010-03-16 | 2010-10-20 | 苏州市玮琪生物科技有限公司 | Urine creatine quantitative test card |
| CN102757893A (en) * | 2011-04-28 | 2012-10-31 | 爱科来株式会社 | Dry test strip and method for measuring creatinine |
| CN103630535A (en) * | 2013-12-03 | 2014-03-12 | 上海科华生物工程股份有限公司 | Dry chemical test paper for quantitatively determining content of human blood albumin |
| CN103901034A (en) * | 2014-04-29 | 2014-07-02 | 中国科学院苏州生物医学工程技术研究所 | Detection reagent strip for detecting microalbumin in urine and preparation method of detection reagent strip |
| CN104198408A (en) * | 2014-08-14 | 2014-12-10 | 上海睿康生物科技有限公司 | Detection kit for determining content of creatinine in serum by enzymic method |
| CN104849270A (en) * | 2015-05-22 | 2015-08-19 | 汇征联合（北京）医疗器械有限公司 | Reagent for determining sodium chloride content in urine, test paper strip thereof, preparation method thereof and purpose thereof |
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106353511A (en) * | 2016-08-19 | 2017-01-25 | 基蛋生物科技股份有限公司 | Urinary microalbumin (U-mALb)/urinary creatinine (U-Cr) integrated assay bigeminy strip and preparation method thereof |
| CN106645758A (en) * | 2017-01-03 | 2017-05-10 | 长沙中生众捷生物技术有限公司 | Detection reagent and test strip for creatinine |
| CN106645132A (en) * | 2017-02-09 | 2017-05-10 | 厦门信道生物技术有限公司 | Test paper for detecting lactose content of sample and preparation method of test paper |
| CN107358033A (en) * | 2017-06-26 | 2017-11-17 | 广东省人民医院(广东省医学科学院) | Appraisal procedure for the intake of Patients with Chronic Kidney Disease dietary protein |
| CN107358033B (en) * | 2017-06-26 | 2019-03-08 | 广东省人民医院(广东省医学科学院) | Appraisal procedure for the intake of Patients with Chronic Kidney Disease dietary protein |
| CN108120714A (en) * | 2017-12-20 | 2018-06-05 | 吉林省汇酉生物技术股份有限公司 | A kind of reagent for detecting urine microdose urine protein and its application |
| CN108120714B (en) * | 2017-12-20 | 2020-11-27 | 吉林省汇酉生物技术股份有限公司 | Reagent for detecting microalbumin in urine and application thereof |
| CN110297081A (en) * | 2018-03-23 | 2019-10-01 | 保生国际生医股份有限公司 | Detection method and its system |
| CN109856131A (en) * | 2019-01-22 | 2019-06-07 | 杭州联晟生物科技有限公司 | It is a kind of for detecting the three-in-one test card and preparation method thereof of uric acid in sample, creatinine, urea simultaneously |
| CN109856131B (en) * | 2019-01-22 | 2022-02-18 | 杭州联晟生物科技有限公司 | Test card for simultaneously detecting uric acid, creatinine and urea in sample and preparation method thereof |
| CN109799331A (en) * | 2019-03-18 | 2019-05-24 | 湖南海源医疗科技股份有限公司 | A kind of Tumor specific factor TSGF analysis strip |
| CN112577940A (en) * | 2019-09-30 | 2021-03-30 | 厦门大学 | Method for rapidly and quantitatively detecting concentration of creatinine in urine at low cost |
| CN110716047A (en) * | 2019-10-24 | 2020-01-21 | 杭州康知生物科技有限公司 | Urine detection kit matched with kidney disease early-screening small-sized full-automatic analyzer |
| CN110716047B (en) * | 2019-10-24 | 2023-03-17 | 杭州康知生物科技有限公司 | Urine detection kit matched with kidney disease early-screening small-sized full-automatic analyzer |
| CN111579797A (en) * | 2020-05-27 | 2020-08-25 | 艾康生物技术(杭州)有限公司 | Detection test paper and application thereof |
| CN113238060A (en) * | 2021-05-08 | 2021-08-10 | 迈克生物股份有限公司 | Kit for predicting or diagnosing myocarditis |
| CN113376383A (en) * | 2021-06-10 | 2021-09-10 | 吉林基蛋生物科技有限公司 | Preparation method of Vc interference resistant urine creatinine test paper |
| CN113418916A (en) * | 2021-07-02 | 2021-09-21 | 安徽惠邦生物工程有限公司 | Creatinine quantitative rapid detection test strip and preparation method thereof |
| CN115201134A (en) * | 2022-09-15 | 2022-10-18 | 吉林大学第一医院 | Creatinine detection kit resistant to piceatannol interference and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105388146B (en) | 2018-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105388146A (en) | Kit for simultaneously detecting sodium, creatinine and microalbumin in urine | |
| CN105334211A (en) | Kit simultaneously detecting sodium and creatinine in urine | |
| US11808777B2 (en) | Method of analyzing diluted biological sample component | |
| US5516700A (en) | Automated urinalysis method | |
| US8883439B2 (en) | Blood component measurement method utilizing hemolyzed whole blood, and kit for the method | |
| CN102175670B (en) | Method for detecting 1,5-dehydration glucitol in blood and kit | |
| CN108287233B (en) | Enzyme-method uric acid detection reagent with strong anti-interference capability | |
| CA2032053C (en) | Method for quantitation of calcium and magnesium | |
| CN104198473A (en) | Stable uric acid detection kit | |
| TW200528717A (en) | A method, reagent and kit for the quantitative analysis of the cholesterol in the very-low-density lipoprotein remnant(vldl remnant) | |
| CN103197084A (en) | Stable glycated serum protein detection reagent and application thereof | |
| CN103048282B (en) | Detection method of bilirubin and detection kit | |
| CN109856128A (en) | A kind of urine glucose detection test paper and preparation method thereof of ascorbic acid interference | |
| CN109613226A (en) | A kind of creatinine assay kit and its application | |
| Jelikić-Stankov et al. | Determination of uric acid in human serum by an enzymatic method using N-methyl-N-(4-aminophenyl)-3-methoxyaniline reagent | |
| CN105572403A (en) | Serum total cholesterol detection kit suitable for full-automatic biochemical analyzer | |
| JP3569139B2 (en) | Diazonium ion assay reagent and method of use | |
| CN104673878B (en) | Kit for measuring concentration ratio of glycated albumin and albumin by virtue of single system | |
| CN108333175B (en) | Total cholesterol detection method | |
| CN103837487A (en) | Uric acid detection method and detection kit | |
| CN114441516A (en) | Uric acid detection kit and preparation method thereof | |
| US5776780A (en) | Method for quantitatively measuring white blood cells esterase activity in urine | |
| JPWO2006030866A1 (en) | Method for quantitative determination of uric acid | |
| JPS585190A (en) | Production of purified animal origin uricase active substance used in measuring uric acid in sample | |
| Blank et al. | Ketone interference in estimation of urinary creatinine; effect on creatinine clearance in diabetic ketocidosis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20160412 Address after: 100044 No. 11 South Main Street, Beijing, Xizhimen Applicant after: PEKING UNIVERSITY PEOPLE'S Hospital Applicant after: BEIJING ZHONGSHENG JINYU DIAGNOSIS TECHNOLOGY Co.,Ltd. Address before: 102200 No. 6, Spark Street, Changping District science and Technology Park, Beijing Applicant before: BEIJING ZHONGSHENG JINYU DIAGNOSIS TECHNOLOGY Co.,Ltd. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230313 Address after: 102200 Standard Workshop No. 6 Xinghuo Street, Changping District Science and Technology Park, Beijing, on the east side of the third floor and the west side of the second floor Patentee after: BEIJING ZHONGSHENG JINYU DIAGNOSIS TECHNOLOGY Co.,Ltd. Address before: 100044 No. 11 South Main Street, Beijing, Xizhimen Patentee before: PEKING UNIVERSITY PEOPLE'S Hospital Patentee before: BEIJING ZHONGSHENG JINYU DIAGNOSIS TECHNOLOGY Co.,Ltd. |