A kind of O-phthalaldehyde disinfectant concentration quick detection test paper and preparation method thereof with
Using
Technical field
The present invention relates to field of chemical detection, in particular to a kind of O-phthalaldehyde disinfectant concentration quick detection test paper and
Preparation method and application.
Background technique
O-phthalaldehyde has many advantages, such as wide sterilization spectrum, efficient, non-corrosive, nonirritant, extensive use as disinfectant
The disinfection of the medical instrument of low temperature high level disinfection is needed in hospital's scope, dialysis, anaesthetic equipment etc..In Sterilization of medical apparatus and instruments process
In, the concentration of o-phthalaldehyde can gradually decrease in the thimerosal of continuous use.The concentration of o-phthalaldehyde is to guarantee that sterilization disappears
Sterilisation purpose is just not achieved when its concentration is lower than 0.3% in the key of toxic effect fruit.In practical applications, general to be examined by test paper
The concentration of O-phthalaldehyde disinfectant is surveyed, but needs to meet three most basic conditions, i.e., easy to operate, rapid reaction is determined
Amount is accurate.
There are two main classes for the Test paper that presently commercially available o-phthalaldehyde thimerosal is equipped with: one kind is by reaction front and back
PH value variation, the concentration of reading thimerosal is compareed with standard color comparison card using the discoloration of acid-base indicator.It is another kind of to be
Yellow substance is generated using amino acid and O-phthalic aldehyde reaction, its concentration is judged after compareing with standard color comparison card.The former lacks
Point is that the pH of reaction end can be influenced by the variation of thimerosal pH value, thus the variation for influencing indicator color cause it is quantitative not
Accurately.Though the test paper of the latter's method preparation is not influenced by front and back pH value is reacted, at present when the reaction of most of test products
Between 1-2min colour developing completely, or even longer time, be unfavorable for medical worker's fast slowdown monitoring at any time.
Therefore, it is particularly important that quick one kind, simplicity, the testing tool of Accurate Determining O-phthalic aldehyde concentration are researched and developed.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of O-phthalaldehyde disinfectant concentration quick detection test paper and
Preparation method and application, solves the problems, such as amino acid and o-phthalaldehyde reaction solution relatively slowly this, its reaction can be accelerated
Rate improves monitoring efficiency.
The technical problem to be solved by the present invention is to what is be achieved through the following technical solutions:
A kind of O-phthalaldehyde disinfectant concentration quick detection test paper, including ground and filter paper, the filter paper are pasted in institute
It states on ground, which is characterized in that fixative solution, solution A and B solution are soaked in the filter paper;The A liquid includes with the following group
Point: 0.2%-4% adsorbent, 0.2%-16% colored indicator, 0.1%-15% water-soluble solid organic acid, 0.02%-5%
Suspension dispersive agent;The B solution includes following parts by weight of component: 0.2%-6% adsorbent and 0.1%-6% suspension dispersive agent,
The binder aqueous solution of 0.2%-10%.
Preferably, in above-mentioned technical proposal, the fixative is carboxymethyl cellulose, hydroxypropyl methyl cellulose, hydroxyl second
One of base cellulose, hydroxypropyl cellulose and their metal salt or multiple combinations.
Preferably, in above-mentioned technical proposal, the adsorbent is poly- selected from white carbon black, the crosslinking of allyl methacrylate class
Conjunction object (E200
(ALLYL METHACRYLATES CROSSPOLYMER)) or ethylene glycol dimethacrylate and dodecyl first
Base acrylate copolymer ((Poly(lauryl methacrylate-co-ethylene glycol
One of))) or multiple combinations dimethacrylate.
Preferably, in above-mentioned technical proposal, the colored indicator selected from one of amino acid esters compound or
Multiple combinations, as glycine methyl ester, glycine ethyl ester, lysine methyl ester, ethyl ester of lysine, threonine methyl, threonine ethyl,
Tryptophan methyl ester, tryptophan ethyl ester, histidine methylester, histidine ethyl ester etc..
Preferably, in above-mentioned technical proposal, the water-soluble solid organic acid be selected from citric acid, tartaric acid, benzene sulfonic acid,
One of p-methyl benzenesulfonic acid and oxalic acid or multiple combinations.
Preferably, in above-mentioned technical proposal, the suspension dispersive agent is selected from anion, nonionic and/or amphoteric ion
Dispersing agent, one in iso-octyl benzene sulfonic acid sodium salt, neopelex, alkyl phenol polyoxyethylene ether, propyl betaine
Kind or a variety of combinations.
One of preferably, in above-mentioned technical proposal, described adhesive is worked as selected from polyvinyl alcohol and/or polyethylene glycol
Or two kinds of combinations.
A kind of preparation method of O-phthalaldehyde disinfectant concentration quick detection test paper, comprising the following steps:
1) prepare plain paper: after the fixative solution for preparing 0.1%-20%, the filter paper for cutting into a certain size is immersed should
In solution, sufficiently after infiltration, 85 DEG C of drying;
2) preparation of A liquid: being respectively 0.2%-4% adsorbent, 0.2%-16% colored indicator, 0.1%- by parts by weight
15% catalyst and 0.02%-5% suspension dispersive agent are added into deionized water, stir evenly;
3) the plain paper by step 1) preparation immerses in A liquid, sufficiently after infiltration, 85 DEG C of drying;
4) preparation of B liquid: respectively by parts by weight be the adsorbent of 0.2%-6%, 0.1%-6% suspension dispersive agent and
The water-soluble binder of 0.1%-10% is added into deionized water, stirs evenly;
5) step 3) treated paper is immersed in B liquid, sufficiently after infiltration, 85 DEG C of drying;
6) step 5) treated paper cutouts are pasted on the ground not absorbed water, is cut at the strip of 0.3-1cm wide
O-phthalaldehyde quick detection test paper is made in the test-strips of 0.3-1cm wide.
A kind of O-phthalaldehyde disinfectant concentration quick detection test paper is in measurement O-phthalaldehyde disinfectant solution concentration
Application.
Preferably, in above-mentioned technical proposal, comprising the following steps:
1) the colour developing block of test paper end is totally submerged in detected solution, keeps 2s, taken out, wipe that the back side is extra to disappear off
Venom, it is horizontal positioned that it is made to develop the color naturally;
2) it is carried out after 20s with standard color comparison card (by the colour developing test paper of the previously prepared ladder concentration of the method for the present invention) pair
According to if test paper shows uniform yellow, O-phthalaldehyde disinfectant solution concentration is 0.3%-0.6%;If test paper shows faint yellow
Or do not develop the color, then O-phthalaldehyde disinfectant solution concentration is less than 0.3%.
The principle of the present invention is that the aldehyde radical based on o-phthalaldehyde can be condensed generation schiff bases with the amino of amino acid esters
Principle, select water-soluble solid organic acid (preferably p-methyl benzenesulfonic acid) be catalyst, accelerate reaction rate.By on test paper
(standard color comparison card is to test ladder concentration in advance with this experimental method to the shade that product shows with standard color comparison card
A series of colorimetric cards being of different shades obtained from o-phthalaldehyde thimerosal) control, judge the concentration of o-phthalaldehyde
Range.Reaction equation is as follows:
The present invention generates yellow substance using o-phthalaldehyde and amino acids substance reaction, and its essence is aldehyde radicals and amino
Reaction.Under conditions of no catalyst or other substances, o-phthalaldehyde carries out relatively slow, system with reacting for amino acid
It is longer at developing time after test paper, it is unfavorable for the progress that medical worker quickly detects work.For this problem, make in the present invention
Use water-soluble solid organic acid as catalyst.Selected water-soluble solid organic acid is water-soluble to form colourless solution, instead
It should be generated afterwards without by-product, will not influence the progress of reaction.The addition of the compound compared with existing method for preparing test paper,
It is not cumbersome, it is simple and easy.The addition of water-soluble solid organic acid not only will not influence the discoloration of test paper, but also accelerate the speed of reaction
Rate shortens developing time, improves the working efficiency of medical worker.This method is simple to operation, and measurement result is accurate, is suitble to
Medical worker monitors the concentration of o-phthalaldehyde thimerosal at any time.
To solve the problems, such as that it is slower that amino acid and o-phthalaldehyde develop the color on test paper, present invention use is to water-soluble solid
Organic acid makes test paper quick colour-developing as catalyst, the reaction rate of catalytic amino acid esters and o-phthalaldehyde, saves detection
Time.The water-soluble solid organic acid is the organic acid for not having oxidisability, soluble easily in water, without side-effects to reacting.This hair
The test paper of bright preparation is not influenced by disinfectant solution pH value, and after picking detected solution, test paper can be tied in 20 seconds
Fruit.If detection is qualified, yellow (can compare with the standard color comparison card prepared in advance) is presented in test paper, if testing result does not conform to
Lattice are then presented faint yellow or are not developed the color.
Above-mentioned technical proposal of the present invention, has the following beneficial effects:
The present invention generates yellow substance using o-phthalaldehyde and amino acids substance reaction, and its essence is aldehyde radicals and amino
Reaction.Under conditions of no catalyst or other substances, o-phthalaldehyde carries out relatively slow, system with reacting for amino acid
It is longer at developing time after test paper, it is unfavorable for the progress that medical worker quickly detects work.For this problem, make in the present invention
Use water-soluble solid organic acid as catalyst.Selected water-soluble solid organic acid is water-soluble to form colourless solution, instead
It should be generated afterwards without by-product, will not influence the progress of reaction.The addition of the compound compared with existing method for preparing test paper,
It is simple and easy.The addition of water-soluble solid organic acid not only will not influence the discoloration of test paper, but also accelerate the rate of reaction, shorten
Developing time (20s can develop the color), improves the working efficiency of medical worker.
O-phthalaldehyde thimerosal concentration quick detection test paper provided by the invention, can quickly and accurately detect adjacent benzene two
The content of formaldehyde.To enable the compound on absorption layer to react rapidly with o-phthalaldehyde, the present invention utilizes amino acid and neighbour
Phthalaldehyde reacts the characteristic that yellow substance can be generated, and selects water-soluble solid organic acid as catalyst, catalytic amino acid
Ester type compound and O-phthalic aldehyde reaction, quickly obtain as a result, shortening detection time.The present invention is conducive to medical worker can
It is easy to operate, quick to monitor the concentration of o-phthalaldehyde at any time, to ensure that the safety of medical apparatus sterilizing effect.
Specific embodiment
Specific embodiments of the present invention are described in detail below, in order to further understand the present invention.
All experimental methods used are conventional method unless otherwise specified in following embodiment.In following embodiment
Material, reagent used etc. can be obtained through commercial channels unless otherwise specified.
Embodiment 1
(1) it prepares plain paper: preparing fixative (carboxymethyl cellulose) solution that mass concentration is 0.1%.
Preparation method: it weighs 1g carboxymethyl cellulose and is dissolved in deionized water, be settled to 1000mL.Filter paper is cut into
The size of 10cm × 10cm size immerses in the solution, sufficiently after infiltration, dries in 50 DEG C.
(2) preparation of solution A: the adsorbent (white carbon black) for being 0.2% containing mass concentration in solution A, 0.2% colour developing
Indicator (glycine methyl ester), 0.1% p-methyl benzenesulfonic acid are catalyst, 0.02% suspension dispersive agent (alkyl phenol polyoxy second
Alkene ether).
Preparation method are as follows: weigh 1g white carbon black, 1g glycine methyl ester, 0.5g p-methyl benzenesulfonic acid, 0.1g alkyl phenol polyoxy second
Alkene ether is settled to 500mL with deionized water.It stirs evenly.
(3) the plain paper prepared in (1) is immersed in solution A, is sufficiently infiltrated.It is dried in 50 DEG C.
(4) preparation of B solution: preparing mass concentration is that 0.2% adsorbent (white carbon black) and 0.2% adhesive are (poly-
Vinyl alcohol) and 0.1% suspension dispersive agent (alkyl phenol polyoxyethylene ether).
Preparation method are as follows: weigh 1g white carbon black, the polyvinyl alcohol water solution that 20g concentration is 5%, 0.5g alkyl phenol polyoxy second
Alkene ether is settled to 500mL with deionized water.It stirs evenly.
(5) paper prepared in (3) is immersed in B solution, is sufficiently infiltrated.It is dried in 50 DEG C.
(6) test paper is cut into the strip of 0.5cm wide, is pasted on the ground not absorbed water, the test of 0.5cm wide is cut into
O-phthalaldehyde quick detection test paper card is made in item.
O-phthalaldehyde disinfectant concentration detection method: the colour developing block that will test card end is totally submerged in detected solution
In, taken out after being kept for 2 seconds, wipe the extra thimerosal of card backside off at once, it is horizontal positioned that it is allowed to develop the color naturally, after 20 seconds with standard
(standard color comparison card is one obtained from testing the o-phthalaldehyde thimerosal of ladder concentration in advance with this experimental method to colorimetric card
The different colorimetric card of the color spectrum depth) control, read the concentration of o-phthalaldehyde.When concentration is within the scope of 0.3%-0.6%,
Test paper shows uniform yellow;When solution concentration is less than 0.3%, shows faint yellow or do not develop the color.
Embodiment 2
(1) plain paper is prepared.Prepare 20% fixative (hydroxyethyl cellulose) solution.
Preparation method: it weighs 200g hydroxyethyl cellulose and is dissolved in deionized water, be settled to 1000mL.Filter paper is cut into
The size of 10cm × 10cm size immerses in the solution, sufficiently after infiltration, dries in 90 DEG C.
(2) preparation of solution A: the adsorbent allyl methacrylate class for being 4% containing mass concentration in solution A is crosslinked
Polymer (E200), 16% colored indicator (histidine methylester), 15% catalyst (citric acid), with
And 5% suspension dispersive agent (iso-octyl benzene sulfonic acid sodium salt).
Preparation method are as follows: weigh 20g allyl methacrylate class cross-linked polymer (E200), 80g group
Propylhomoserin methyl esters, 75g citric acid, 25g iso-octyl benzene sulfonic acid sodium salt are settled to 500mL with deionized water.It stirs evenly.
(3) the plain paper prepared in (1) is immersed in solution A, is sufficiently infiltrated.It is dried in 90 DEG C.
(4) preparation of B solution: the adsorbent allyl methacrylate class crosslinking for being wherein 6% containing mass concentration gathers
Conjunction object () and 10% adhesive (polyethylene glycol) and 6% suspension dispersive agent (iso-octyl benzene sulphur E200
Sour sodium).
Preparation method are as follows: weigh 30gE200,50g polyethylene glycol, 30g iso-octyl benzene sulfonic acid sodium salt, spend
Ionized water is settled to 500mL.It stirs evenly.
(5) paper prepared in (3) is immersed in B solution, is sufficiently infiltrated.It is dried in 90 DEG C.
(6) test paper is cut into the strip of 1cm wide, is pasted on the ground not absorbed water, is cut into the test-strips of 1cm wide, i.e.,
O-phthalaldehyde quick detection test paper card is made.
O-phthalaldehyde disinfectant concentration detection method: the colour developing block that will test card end is totally submerged in detected solution
In, taken out after being kept for 2 seconds, wipe the extra thimerosal of card backside off at once, it is horizontal positioned that it is allowed to develop the color naturally, after 20 seconds with mark
(standard color comparison card is tested in advance obtained from the o-phthalaldehyde thimerosal of ladder concentration with this experimental method to quasi- colorimetric card
A series of colorimetric cards being of different shades) control, read the concentration of o-phthalaldehyde.When concentration is in 0.3%-0.6% range
Interior, test paper shows uniform yellow;When solution concentration is less than 0.3%, shows faint yellow or do not develop the color.
Embodiment 3
(1) it prepares plain paper: preparing fixative (carboxymethyl cellulose) solution that mass concentration is 0.5%.
Preparation method: it weighs 5g carboxymethyl cellulose and is dissolved in deionized water, be settled to 1000mL.Filter paper is cut into
The size of 10cm × 10cm size immerses in the solution, sufficiently after infiltration, dries in 85 DEG C.
(2) preparation of solution A: the adsorbent (white carbon black) for being 2% containing mass concentration in solution A, 10% colour developing refer to
Show agent (glycine ethyl ester and the combination of L-Histidine methyl esters), 3% catalyst (p-methyl benzenesulfonic acid), 0.1% suspended dispersed
Agent (neopelex).
Preparation method are as follows: weigh 10g white carbon black, 40g glycine ethyl ester, 10g L-Histidine methyl esters, 15g is to toluene sulphur
Acid, 0.5g neopelex are settled to 500mL with deionized water.It stirs evenly.
(3) the plain paper prepared in (1) is immersed in solution A, is sufficiently infiltrated.It is dried in 85 DEG C.
(4) preparation of B solution: being wherein that 2% adsorbent (white carbon black) and 4% adhesive are (poly- containing mass concentration
Vinyl alcohol) and 0.1% suspension dispersive agent (neopelex).
Preparation method are as follows: weigh 10g white carbon black, the polyvinyl alcohol water solution that 400g concentration is 5%, 0.5g detergent alkylate
Sodium sulfonate is settled to 500mL with deionized water, stirs evenly.
(5) paper prepared in (3) is immersed in B solution, is sufficiently infiltrated.It is dried in 85 DEG C.
(6) test paper is cut into the strip of 1cm wide, is pasted on the ground not absorbed water, is cut into the test-strips of 1cm wide, i.e.,
O-phthalaldehyde quick detection test paper card is made.
O-phthalaldehyde disinfectant concentration detection method: the colour developing block that will test card end is totally submerged in detected solution
In, taken out after being kept for 2 seconds, wipe the extra thimerosal of card backside off at once, it is horizontal positioned that it is allowed to develop the color naturally, after 20 seconds with mark
(standard color comparison card is tested in advance obtained from the o-phthalaldehyde thimerosal of ladder concentration with this experimental method to quasi- colorimetric card
A series of colorimetric cards being of different shades) control, read the concentration of o-phthalaldehyde.When concentration is in 0.3%-0.6% range
Interior, test paper shows uniform yellow;When solution concentration is less than 0.3%, shows faint yellow or do not develop the color.
Embodiment 4
(1) it prepares plain paper: preparing fixative (hydroxypropyl cellulose) solution that mass concentration is 5%.
Preparation method: it weighs 50g hydroxypropyl cellulose and is dissolved in deionized water, be settled to 1000mL.Filter paper is cut into
The size of 10cm × 10cm size immerses in the solution, sufficiently after infiltration, dries in 60 DEG C.
(2) preparation of solution A: in solution A containing mass concentration be 1% adsorbent ethylene glycol dimethacrylate with
Dodecyl methyl acrylate copolymer2% colored indicator (lysine methyl ester), 5% catalyst
(tartaric acid), 2% suspension dispersive agent (propyl betaine).
Preparation method are as follows: weigh 5g10g lysine methyl ester, 25g tartaric acid, 10g propyl betaine are spent
Ionized water is settled to 500mL.It stirs evenly.
(3) the plain paper prepared in (1) is immersed in solution A, is sufficiently infiltrated.It is dried in 60 DEG C.
(4) preparation of B solution: the adsorbent ethylene glycol dimethacrylate and ten for being wherein 1% containing mass concentration
Dialkyl methyl acrylate copolymer2% adhesive (polyethylene glycol) and 2% suspension dispersive agent
(propyl betaine).
Preparation method are as follows: weigh 5g10g polyethylene glycol, 10g propyl betaine, is settled to deionized water
500mL is stirred evenly.
(5) paper prepared in third step is immersed in B solution, is sufficiently infiltrated.It is dried in 60 DEG C.
(6) test paper is cut into the strip of 0.8cm wide, is pasted on the ground not absorbed water, the test of 0.8cm wide is cut into
O-phthalaldehyde quick detection test paper card is made in item.
O-phthalaldehyde disinfectant concentration detection method: the colour developing block that will test card end is totally submerged in detected solution
In, taken out after being kept for 2 seconds, wipe the extra thimerosal of card backside off at once, it is horizontal positioned that it is allowed to develop the color naturally, after 20 seconds with mark
(standard color comparison card is tested in advance obtained from the o-phthalaldehyde thimerosal of ladder concentration with this experimental method to quasi- colorimetric card
A series of colorimetric cards being of different shades) control, read the concentration of o-phthalaldehyde.When concentration is in 0.3%-0.6% range
Interior, test paper shows uniform yellow;When solution concentration is less than 0.3%, shows faint yellow or do not develop the color.
Embodiment 5
(1) it prepares plain paper: preparing 10% fixative (hydroxyethyl cellulose) solution.
Preparation method: it weighs 100g hydroxyethyl cellulose and is dissolved in deionized water, be settled to 1000mL.Filter paper is cut into
The size of 10cm × 10cm size immerses in the solution, sufficiently after infiltration, dries in 70 DEG C.
(2) preparation of solution A: the adsorbent (white carbon black) for being 3% containing mass concentration in solution A, 8% colour developing instruction
Agent (glycine methyl ester and L-Histidine ethyl ester), 10% catalyst (oxalic acid), 3% suspension dispersive agent (alkyl phenol polyoxy second
Alkene ether).
Preparation method are as follows: weigh 15g white carbon black, 25g glycine methyl ester, 15g L-Histidine ethyl ester, 50g oxalic acid, 15g alkane
Base phenol polyethenoxy ether is settled to 500mL with deionized water.It stirs evenly.
(3) the plain paper prepared in the first step is immersed in solution A, is sufficiently infiltrated.It is dried in 70 DEG C.
(4) preparation of B solution: wherein containing 3% adsorbent (white carbon black) and 5% adhesive (polyethylene glycol), with
And 3% suspension dispersive agent (alkyl phenol polyoxyethylene ether).
Preparation method are as follows: weigh 15g white carbon black, 25g Aqueous Solutions of Polyethylene Glycol, 15g alkyl phenol polyoxyethylene ether, spend from
Sub- water is settled to 500mL, stirs evenly.
(5) paper prepared in third step is immersed in B solution, is sufficiently infiltrated.It is dried in 70 DEG C.
(6) test paper is cut into the strip of 1cm wide, is pasted on the ground not absorbed water, is cut into the test-strips of 1cm wide, i.e.,
O-phthalaldehyde quick detection test paper card is made.
O-phthalaldehyde disinfectant concentration detection method: the colour developing block that will test card end is totally submerged in detected solution
In, taken out after being kept for 2 seconds, wipe the extra thimerosal of card backside off at once, it is horizontal positioned that it is allowed to develop the color naturally, after 20 seconds with mark
(standard color comparison card is tested in advance obtained from the o-phthalaldehyde thimerosal of ladder concentration with this experimental method to quasi- colorimetric card
A series of colorimetric cards being of different shades) control, read the concentration of o-phthalaldehyde.When concentration is in 0.3%-0.6% range
Interior, test paper shows uniform yellow;When solution concentration is less than 0.3%, shows faint yellow or do not develop the color.
Comparative example 1
(1) it prepares plain paper: preparing fixative (carboxymethyl cellulose) solution that mass concentration is 1%.
Preparation method: it weighs 10g carboxymethyl cellulose and is dissolved in deionized water, be settled to 1000mL.Filter paper is cut into
The size of 10cm × 10cm size immerses in the solution, sufficiently after infiltration, dries in 85 DEG C.
(2) preparation of solution A: in solution A containing 2% adsorbent (white carbon black), 10% colored indicator (glycine
Methyl esters), 1% suspension dispersive agent (neopelex).
Preparation method are as follows: weigh 10g white carbon black, 50g glycine methyl ester, 5g neopelex uses deionized water
It is settled to 500mL.It stirs evenly.
(3) the plain paper prepared in the first step is immersed in solution A, is sufficiently infiltrated.It is dried in 85 DEG C.
(4) preparation of B solution: wherein containing 2% adsorbent (white carbon black) and 4% adhesive (polyethylene glycol), with
And 1% suspension dispersive agent (neopelex).
Preparation method are as follows: weigh 10g white carbon black, 20g Aqueous Solutions of Polyethylene Glycol, 5g neopelex, spend from
Sub- water is settled to 500mL, stirs evenly.
(5) paper prepared in third step is immersed in B solution, is sufficiently infiltrated.It is dried in 85 DEG C.
(6) test paper is cut into the strip of 1cm wide, is pasted on the ground not absorbed water, is cut into the test-strips of 1cm wide, i.e.,
O-phthalaldehyde quick detection test paper card is made.
O-phthalaldehyde disinfectant concentration detection method: the colour developing block that will test card end is totally submerged in detected solution
In, taken out after being kept for 2 seconds, wipe the extra thimerosal of card backside off at once, it is horizontal positioned that it is allowed to develop the color naturally, after 2 minutes and
Standard color comparison card control, reads the concentration of o-phthalaldehyde.When concentration is within the scope of 0.3%-0.6%, test paper is aobvious uniform yellow
Color;When solution concentration is less than 0.3%, shows faint yellow or do not develop the color.
O-phthalaldehyde disinfectant concentration detection method of the invention, it is only necessary to 20s can be with standard color comparison card pair
According to, and comparative example is then needed just to can be carried out for 2 minutes and be compared, required time is longer.
Although the present invention is disclosed as above with embodiment, so it is not intended to limit the present invention, any those skilled in the art
Member, without departing from the spirit and scope of the present invention, can make a variety of different selections and modification, therefore protection model of the invention
It encloses and is limited by claims and its equivalents.