CN106970078B - A kind of test paper detecting o-phthalaldehyde and its preparation and detection method - Google Patents
A kind of test paper detecting o-phthalaldehyde and its preparation and detection method Download PDFInfo
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Abstract
The invention belongs to disinfectant detection technique field, it is related to a kind of test paper for detecting o-phthalaldehyde and its preparation and detection method.The test paper is fixed with colored indicator on plain paper, and the colored indicator is selected from one of amino acid esters compound, arylamine and/or fat primary amine or a variety of combinations.Utilize the test paper of detection o-phthalaldehyde of the invention and its preparation and detection method, it can make the test paper being prepared in detecting thimerosal when O-phthalic aldehyde concentration, reduce dissolution of the colored indicator fixed on test paper in thimerosal, it is small to make o-phthalaldehyde Concentration Testing result be influenced by test paper with thimerosal time of contact, developing time, keep testing result more true and reliable.
Description
Technical field
The invention belongs to disinfectant detection technique field, it is related to a kind of test paper for detecting o-phthalaldehyde and its preparation and inspection
Survey method.
Background technique
O-phthalaldehyde (O-phthalaldehyde, OPA) is a kind of common disinfectant, has property stabilization, sterilization
The advantages that spectrum is wide, sterilizing power is strong and nonirritant to skin and mucosa, to metal low-corrosiveness, therefore in medical instrument cold sterilization
Field is widely used.To guarantee Disinfection Effect, it is specified that the effective concentration of o-phthalaldehyde is greater than 0.3% in thimerosal
(m/v) it can be used when.
It is domestic in recent years for O-phthalaldehyde disinfectant product can be made quickly to be detected in market surpervision and routine use
It all is directed to the Concentration Testing test paper of O-phthalaldehyde disinfectant product in research and development outside, existing test paper is broadly divided into two classes.
The testing principle of first kind Test paper is as follows.Sodium sulfite and O-phthalic aldehyde reaction generate alkali, make reactant
It is that pH value increases；After reacting again by a certain amount of carboxylic group introduced on test paper with alkali, indicate phenolphthalein by remaining alkali
Agent discoloration carrys out o-phthalaldehyde concentration range in judgement sample.The shortcomings that this kind of test paper, there is two, first is that reaction front and back pH value can be with
The variation of disinfectant pH value itself and change, influence the color change of indicator, cause measurement inaccurate；Second is that if O-phthalic
There are buffer systems in aldehyde disinfectant, then the test paper will be examined completely and not measure o-phthalaldehyde.
The testing principle of second class Test paper is as follows.It is reacted using aldehydes with amino acids and generates yellow substance
Afterwards, the concentration range measurement for carrying out o-phthalaldehyde is compareed with standard color comparison card.The shortcomings that this kind of test paper be pick it is detected
Partial amino-acid substance can be quickly soluble in disinfectant solution when solution, and amino is caused to be lost, and test paper color development is caused to shoal,
To make o-phthalaldehyde quantify inaccuracy.
Summary of the invention
Primary and foremost purpose of the invention is to provide a kind of test paper for detecting o-phthalaldehyde, can make the test paper being prepared
In detecting thimerosal when O-phthalic aldehyde concentration, dissolution of the colored indicator fixed on test paper in thimerosal is reduced, from
And it is small to influence o-phthalaldehyde Concentration Testing result with thimerosal time of contact, developing time by test paper, makes testing result more
Add true and reliable.
In order to achieve this, the present invention provides a kind of test paper for detecting o-phthalaldehyde, institute in the embodiment on basis
The test paper stated is fixed with colored indicator on plain paper, the colored indicator be selected from amino acid esters compound, arylamine and/
Or one of fat primary amine or a variety of combinations.
It can be condensed with the amino of amino acid esters compound the present invention is based on the aldehyde radical of o-phthalaldehyde and generate yellow Schiff
The principle of alkali carries out, and is compareed by the shade that product on test paper shows with standard color comparison card, judges o-phthalaldehyde
Concentration range.
In a preferred embodiment, the present invention provides a kind of test paper for detecting o-phthalaldehyde, wherein described
Amino acid esters compound is selected from glycine methyl ester, glycine ethyl ester, lysine methyl ester, ethyl ester of lysine, threonine methyl, Soviet Union
Propylhomoserin ethyl ester, tryptophan methyl ester, tryptophan ethyl ester, histidine methylester, histidine ethyl ester, the different monooctyl ester of glycine, glycine 12
One of ester, phenyalanine methyl ester and their salt or a variety of combinations；
The arylamine, fat primary amine are selected from one of open-chain crown ether, dodecyl polyaniline, octyl amine or a variety of
Combination.
In a preferred embodiment, the present invention provides a kind of test paper for detecting o-phthalaldehyde, wherein every institute
The total amount of the amino acid esters compound, arylamine and/or fat primary amine is 0.2-16mg on the test paper stated.
In a kind of more preferred embodiment, the present invention provides a kind of test paper for detecting o-phthalaldehyde, wherein often
The total amount of the amino acid esters compound, arylamine and/or fat primary amine is 0.5-5mg on the test paper of Zhang Suoshu.
A second object of the present invention is to provide the preparation methods of the test paper of aforementioned detection o-phthalaldehyde, can make to make
Standby obtained test paper when O-phthalic aldehyde concentration, reduces colored indicator fixed on test paper in thimerosal in detecting thimerosal
In dissolution make so that it is small to influence o-phthalaldehyde Concentration Testing result with thimerosal time of contact, developing time by test paper
Testing result is more true and reliable.
In order to achieve this, the present invention provides the test paper of aforementioned detection o-phthalaldehyde in the embodiment on basis
Preparation method includes the following steps:
(1) it prepares plain paper: preparing the fixative solution of mass volume ratio concentration 0.1%-20%, a certain size will be cut into
Filter paper immerse in the solution, sufficiently infiltration after, in 50-90 DEG C dry；
(2) preparation of solution A: the adsorbent containing mass volume ratio concentration 0.2%-4% in solution A, 0.2%-16%
Colored indicator, the suspension dispersive agent of 0.02%-5% stirs evenly；
(3) the plain paper that step (1) is prepared immerses in solution A, sufficiently infiltrates, and dries in 50-90 DEG C；
(4) preparation of B solution: the adsorbent containing mass volume ratio concentration 0.2%-6% in B solution, 0.1%-6%'s
Suspension dispersive agent, the adhesive of 0.2%-10%, stirs evenly；
(5) paper that step (3) is prepared immerses in B solution, sufficiently infiltrates, and dries in 50-90 DEG C；
(6) the paper cutouts strip that step (5) is prepared, pastes on the ground not absorbed water, is cut into test-strips
O-phthalaldehyde quick detection test paper is made in (such as 0.3-1cm wide).
In a preferred embodiment, the present invention provides the preparation method of the test paper of aforementioned detection o-phthalaldehyde,
Wherein the fixative is water-soluble high-molecular material, is selected from carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose
And one of their metal salt or a variety of combinations.
In a preferred embodiment, the present invention provides the preparation method of the test paper of aforementioned detection o-phthalaldehyde,
The mass volume ratio concentration for the fixative solution wherein prepared in step (1) is 0.5-5%.
In a preferred embodiment, the present invention provides the preparation method of the test paper of aforementioned detection o-phthalaldehyde,
Wherein the adsorbent is white or colourless adsorbent, is selected from white carbon black, allkyl methacrylate cross-linked polymer
(ALLYL METHACRYLATES CROSSPOLYMER, commercially produced product is for example) or poly- (metering system E200
Sour lauryl -co- ethylene glycol dimethacrylate) (Poly (lauryl methacrylate-co-ethylene glycol
Dimethacrylate), commercially produced product is for exampleOne of or a variety of combinations 6603).
In a preferred embodiment, the present invention provides the preparation method of the test paper of aforementioned detection o-phthalaldehyde,
The mass volume ratio concentration of adsorbent is 0.2- in the solution A and B solution wherein prepared in step (2) and step (4)
2%.
In a preferred embodiment, the present invention provides the preparation method of the test paper of aforementioned detection o-phthalaldehyde,
The mass volume ratio concentration of colored indicator is 0.5-5% in the solution A wherein prepared in step (2).
In a preferred embodiment, the present invention provides the preparation method of the test paper of aforementioned detection o-phthalaldehyde,
Wherein the suspension dispersive agent is anion, nonionic and/or amphoteric ion dispersing agent, is selected from iso-octyl benzene sulfonic acid sodium salt, ten
One of dialkyl benzene sulfonic acids sodium, alkyl phenol polyoxyethylene ether, propyl betaine or a variety of combinations.
In a preferred embodiment, the present invention provides the preparation method of the test paper of aforementioned detection o-phthalaldehyde,
The mass volume ratio concentration of suspension dispersive agent is 0.05-1% in the solution A wherein prepared in step (2)；Step (4)
The mass volume ratio concentration of suspension dispersive agent is 0.1-2% in the B solution of middle preparation.
In a preferred embodiment, the present invention provides the preparation method of the test paper of aforementioned detection o-phthalaldehyde,
Wherein the adhesive is the water-soluble high-molecular material with bond properties, is selected from polyvinyl alcohol and/or polyethylene glycol.
In a preferred embodiment, the present invention provides the preparation method of the test paper of aforementioned detection o-phthalaldehyde,
The mass volume ratio concentration of adhesive is 0.5-5% in the B solution wherein prepared in step (4).
Third object of the present invention is to provide the test paper of aforementioned detection o-phthalaldehyde for O-phthalic aldehyde concentration
Detection method is reduced fixed on test paper with that can make the test paper being prepared in detecting thimerosal when O-phthalic aldehyde concentration
Dissolution of the colored indicator in thimerosal, thus when contacting o-phthalaldehyde Concentration Testing result by test paper and thimerosal
Between, developing time influence it is small, keep testing result more true and reliable.
In order to achieve this, the test paper that the present invention provides aforementioned detection o-phthalaldehyde is used in the embodiment on basis
In the detection method of O-phthalic aldehyde concentration, the detection method in turn includes the following steps:
(1) test paper is immersed in solution to be measured and carries out haptoreaction；
(2) the haptoreaction time, after (such as 2 seconds) took out the test paper, removed solution to be measured extra on test paper；
(3) being horizontally arranged test paper makes it develop the color naturally, compares with standard color comparison card, obtains the concentration of o-phthalaldehyde.
In a preferred embodiment, the present invention provides the test paper of aforementioned detection o-phthalaldehyde for O-phthalic
The detection method of aldehyde concentration, wherein the haptoreaction time described in step (1) is 2-4s；It is naturally aobvious described in step (3)
The color time is 1-5min.
Overall beneficial effect of the invention is, utilizes the test paper of detection o-phthalaldehyde of the invention and its preparation and inspection
Survey method can make the test paper being prepared in detecting thimerosal when O-phthalic aldehyde concentration, reduce fixed on test paper show
Dissolution of the color indicator in thimerosal, thus make o-phthalaldehyde Concentration Testing result by test paper and thimerosal time of contact,
Developing time influence is small, keeps testing result more true and reliable.
The specific beneficial effect of the present invention embodies as follows:
1, the present invention can detecte O-phthalaldehyde disinfectant mass volume ratio concentration range be 0.3%-0.6% (when
When o-phthalaldehyde mass volume ratio concentration is within the scope of 0.3%-0.6%, test paper shows uniform yellow；When o-phthalaldehyde matter
When measuring volume by volume concentration less than 0.3%, test paper shows faint yellow or does not develop the color), O-phthalic is judged according to the depth that test paper changes colour
The concentration range of aldehyde.For being various amino acid with the primary raw material of o-phthalaldehyde reaction solution on Test paper in the present invention
Ester, compared to traditional colour developing test paper use amino acids for raw material for, the present invention used in amino acid esters
Rate of dissolution is low in aqueous solution for raw material, and compound when picking tested liquid on test paper is spread relatively slowly, especially into water
Become water-insoluble acetal later with aldehyde reaction, ensure that the amino amount on test paper, to enable o-phthalaldehyde therewith
Sufficiently reaction, colour developing are easier to differentiate, quantitative more acurrate.
2, the amino acid esters raw material range of choice used in the present invention is extensive, certain amino acid difference chain both may be selected
Long esters, such as glycine methyl ester, glycine ethyl ester, histidine methylester, histidine ethyl ester etc.；Also different aminoacids kind may be selected
The carboxylate of class, such as ethyl ester of lysine, leucinethylester etc..In selection, can simultaneously from the condition of reaction, raw material at
Originally, reaction time speed etc. comprehensively considers selection optimum feed stock.
3, the test paper caused by the avoidable difference because picking the time when operation of the present invention shows color difference.Traditional test paper
When testing same O-phthalaldehyde disinfectant, if picking, the time is slightly long, and the amino acids substance loss of attachment can increase, and takes out examination
It develops the color after paper slightly shallow；If it is relatively short to pick the time, develop the color relatively slightly deep, this will lead to same fluid to be measured and is difficult to accurately sentence
Break its concentration.Solubility is low in amino-acid ester compound aqueous solution used in the present invention, effectively prevents temporal because picking
Shade caused by difference is different, and tester is made to be easier to judge the concentration of o-phthalaldehyde, easy to operation.
Specific embodiment
A specific embodiment of the invention is further illustrated with reference to embodiments, but embodiment party of the invention
Formula is not limited to following embodiment.The concentration hereinafter occurred, as being that mass volume ratio (m/v) is dense without particularly pointing out
Degree.
Embodiment 1: the preparation (one) of O-phthalaldehyde disinfectant Concentration Testing test paper
O-phthalaldehyde disinfectant Concentration Testing test paper is prepared as follows.
1, it prepares plain paper: preparing 0.5% fixative solution first.Preparation method: weighing 5g carboxymethyl cellulose, by it
It is dissolved in deionized water, is settled to 1000mL.Filter paper is cut into the size of 10cm × 10cm size, is immersed in the solution, sufficiently
After infiltration, dried in 50 DEG C.
2, the preparation of solution A: contain 0.2% adsorbent, 0.5% colored indicator, 0.05% suspended dispersed in solution A
Agent.Preparation method are as follows: 1g white carbon black, 1.56g glycine methyl ester hydrochloride, 0.94g histidine methylester are added into deionized water
Hydrochloride, 0.25g alkyl phenol polyoxyethylene ether are settled to 500mL with deionized water, stir evenly.
3, the plain paper prepared in step 1 is immersed in solution A, is sufficiently infiltrated, dried in 50 DEG C.
4, the preparation of B solution: contain 0.2% adsorbent and 0.5% water-soluble binder and 0.1% outstanding in B solution
Floating dispersing agent.Preparation method are as follows: the addition 1g white carbon black into deionized water, the polyvinyl alcohol water solution that 50g concentration is 5%,
0.5g alkyl phenol polyoxyethylene ether is settled to 500mL with deionized water, stirs evenly.
5, the paper prepared in step 3 is immersed in B solution, is sufficiently infiltrated, dried in 50 DEG C.
6, test paper is cut into the strip of 1cm wide, is pasted on the ground not absorbed water, the test-strips of 1cm wide is cut into, that is, makes
At o-phthalaldehyde quick detection test paper card.
The mass percentage content of colored indicator is 0.5mg in the test card prepared according to the above method.
Embodiment 2: the preparation (two) of O-phthalaldehyde disinfectant Concentration Testing test paper
O-phthalaldehyde disinfectant Concentration Testing test paper is prepared as follows.
1, it prepares plain paper: preparing 5% fixative solution first.Preparation method: weighing 50g hydroxyethyl cellulose, by it
It is dissolved in deionized water, is settled to 1000mL.Filter paper is cut into the size of 10cm × 10cm size, is immersed in the solution, sufficiently
After infiltration, dried in 90 DEG C.
2, the preparation of solution A: contain 2% adsorbent, 5% colored indicator, 1% suspension dispersive agent in solution A.Preparation side
Method are as follows: 10g white carbon black, 12.5g lysine methyl ester hydrochloride, 12.5g threonine ethyl hydrochloride, 5g are added into deionized water
Neopelex is settled to 500mL with deionized water, stirs evenly.
3, the plain paper prepared in step 1 is immersed in solution A, is sufficiently infiltrated, dried in 90 DEG C.
4, the preparation of B solution: water-soluble binder and 2% suspended dispersed in B solution containing 2% adsorbent and 5%
Agent.Preparation method are as follows: 10g white carbon black, the Aqueous Solutions of Polyethylene Glycol that 125g concentration is 20%, 10g ten are added into deionized water
Dialkyl benzene sulfonic acids sodium is settled to 500mL with deionized water, stirs evenly.
5, the paper prepared in step 3 is immersed in B solution, is sufficiently infiltrated, dried in 90 DEG C.
6, test paper is cut into the strip of 1cm wide, is pasted on the ground not absorbed water, the test-strips of 1cm wide is cut into, that is, makes
At o-phthalaldehyde quick detection test paper card.
The mass percentage content of colored indicator is 5mg in the test card prepared according to the above method.
Embodiment 3: the preparation (three) of O-phthalaldehyde disinfectant Concentration Testing test paper
O-phthalaldehyde disinfectant Concentration Testing test paper is prepared as follows.
1, it prepares plain paper: preparing 0.5% fixative solution first.Preparation method: weighing 5g carboxymethyl cellulose, by it
It is dissolved in deionized water, is settled to 1000mL.Filter paper is cut into the size of 10cm × 10cm size, is immersed in the solution, sufficiently
After infiltration, dried in 50 DEG C.
2, the preparation of solution A: contain 0.2% adsorbent, 0.5% colored indicator, 0.05% suspended dispersed in solution A
Agent.Preparation method are as follows: 1g is added into deionized waterE200,1.56g tryptophan methyl ester hydrochloride, 0.94g
Tryptophan carbethoxy hydrochloride, 0.25g iso-octyl benzene sulfonic acid sodium salt, is settled to 500mL with deionized water, stirs evenly.
3, the plain paper prepared in step 1 is immersed in solution A, is sufficiently infiltrated, dried in 50 DEG C.
4, the preparation of B solution: contain 0.2% adsorbent and 0.5% water-soluble binder and 0.1% outstanding in B solution
Floating dispersing agent.Preparation method are as follows: 1g is added into deionized waterThe polyvinyl alcohol that E200,50g concentration are 5%
Aqueous solution, 0.5g iso-octyl benzene sulfonic acid sodium salt, is settled to 500mL with deionized water, stirs evenly.
5, the paper prepared in step 3 is immersed in B solution, is sufficiently infiltrated, dried in 50 DEG C.
6, test paper is cut into the strip of 1cm wide, is pasted on the ground not absorbed water, the test-strips of 1cm wide is cut into, that is, makes
At o-phthalaldehyde quick detection test paper card.
The mass percentage content of colored indicator is 0.5mg in the test card prepared according to the above method.
Embodiment 4: the preparation (four) of O-phthalaldehyde disinfectant Concentration Testing test paper
O-phthalaldehyde disinfectant Concentration Testing test paper is prepared as follows.
1, it prepares plain paper: preparing 5% fixative solution first.Preparation method: weighing 50g hydroxyethyl cellulose, by it
It is dissolved in deionized water, is settled to 1000mL.Filter paper is cut into the size of 10cm × 10cm size, is immersed in the solution, sufficiently
After infiltration, dried in 90 DEG C.
2, the preparation of solution A: contain 2% adsorbent, 5% colored indicator, 1% suspension dispersive agent in solution A.Preparation side
Method are as follows: 10g is added into deionized water6603,12.5g glycine methyl esters, 12.5g glycine ethyl ester, 5g 12
Sodium alkyl benzene sulfonate is settled to 500mL with deionized water, stirs evenly.
3, the plain paper prepared in step 1 is immersed in solution A, is sufficiently infiltrated, dried in 90 DEG C.
4, the preparation of B solution: water-soluble binder and 2% suspended dispersed in B solution containing 2% adsorbent and 5%
Agent.Preparation method are as follows: 10g is added into deionized waterThe polyethylene glycol that 6603,125g concentration are 20% is water-soluble
Liquid, 10g neopelex are settled to 500mL with deionized water, stir evenly.
5, the paper prepared in step 3 is immersed in B solution, is sufficiently infiltrated, dried in 90 DEG C.
6, test paper is cut into the strip of 1cm wide, is pasted on the ground not absorbed water, the test-strips of 1cm wide is cut into, that is, makes
At o-phthalaldehyde quick detection test paper card.
The mass percentage content of colored indicator is 5mg in the test card prepared according to the above method.
Embodiment 5: the preparation (five) of O-phthalaldehyde disinfectant Concentration Testing test paper
O-phthalaldehyde disinfectant Concentration Testing test paper is prepared as follows.
1, it prepares plain paper: preparing 2% fixative solution first.Preparation method: weighing 20g hydroxypropyl cellulose, by it
It is dissolved in deionized water, is settled to 1000mL.Filter paper is cut into the size of 10cm × 10cm size, is immersed in the solution, sufficiently
After infiltration, dried in 85 DEG C.
2, the preparation of solution A: contain 0.5% adsorbent, 3% colored indicator, 0.3% suspension dispersive agent in solution A.Match
Method processed are as follows: 2.5g white carbon black, 10g open-chain crown ether, 5g dodecyl polyaniline, 1.5g propyl beet are added into deionized water
Alkali is settled to 500mL with deionized water, stirs evenly.
3, the plain paper prepared in step 1 is immersed in solution A, is sufficiently infiltrated, dried in 85 DEG C.
4, the preparation of B solution: water-soluble binder and 0.5% suspension in B solution containing 0.5% adsorbent and 2%
Dispersing agent.Preparation method are as follows: the addition 2.5g white carbon black into deionized water, the polyvinyl alcohol water solution that 50g concentration is 20%,
2.5g propyl betaine is settled to 500mL with deionized water, stirs evenly.
5, the paper prepared in third step is immersed in B solution, is sufficiently infiltrated, dried in 85 DEG C.
6, test paper is cut into the strip of 1cm wide, is pasted on the ground not absorbed water, the test-strips of 1cm wide is cut into, that is, makes
At o-phthalaldehyde quick detection test paper card.
The mass percentage content of colored indicator is 3mg in the test card prepared according to the above method.
Embodiment 6: the preparation (six, reference examples) of O-phthalaldehyde disinfectant Concentration Testing test paper
O-phthalaldehyde disinfectant Concentration Testing test paper is prepared as follows.
1, it prepares plain paper: preparing 0.5% fixative solution first.Preparation method: weighing 5g carboxymethyl cellulose, by it
It is dissolved in deionized water, is settled to 1000mL.Filter paper is cut into the size of 10cm × 10cm size, is immersed in the solution, sufficiently
After infiltration, dried in 50 DEG C.
2, the preparation of solution A: contain 0.2% adsorbent, 0.5% colored indicator, 0.05% suspended dispersed in solution A
Agent.Preparation method are as follows: 1g white carbon black, 1.56g glycine, 0.94g histidine, 0.25g alkyl phenol polyoxy are added into ionized water
Vinethene is settled to 500mL with deionized water, stirs evenly.
3, the plain paper prepared in step 1 is immersed in solution A, is sufficiently infiltrated, dried in 50 DEG C.
4, the preparation of B solution: contain 0.2% adsorbent and 0.5% water-soluble binder and 0.1% outstanding in B solution
Floating dispersing agent.Preparation method are as follows: the addition 1g white carbon black into deionized water, the polyvinyl alcohol water solution that 50g concentration is 5%,
0.5g alkyl phenol polyoxyethylene ether is settled to 500mL with deionized water, stirs evenly.
5, the paper prepared in step 3 is immersed in B solution, is sufficiently infiltrated, dried in 50 DEG C.
6, test paper is cut into the strip of 1cm wide, is pasted on the ground not absorbed water, the test-strips of 1cm wide is cut into, that is, makes
At o-phthalaldehyde quick detection test paper card.
The mass percentage content of colored indicator is 0.5mg in the test card prepared according to the above method.
Embodiment 7: the preparation (seven, reference examples) of O-phthalaldehyde disinfectant Concentration Testing test paper
O-phthalaldehyde disinfectant Concentration Testing test paper is prepared as follows.
1, it prepares plain paper: preparing 5% fixative solution first.Preparation method: weighing 50g hydroxyethyl cellulose, by it
It is dissolved in deionized water, is settled to 1000mL.Filter paper is cut into the size of 10cm × 10cm size, is immersed in the solution, sufficiently
After infiltration, dried in 90 DEG C.
2, the preparation of solution A: contain 2% adsorbent, 5% colored indicator, 1% suspension dispersive agent in solution A.Preparation side
Method are as follows: 10g white carbon black, 12.5g lysine, 12.5g threonine, 5g neopelex, use are added into deionized water
Deionized water is settled to 500mL, it stirs evenly.
3, the plain paper prepared in step 1 is immersed in solution A, is sufficiently infiltrated, dried in 90 DEG C.
4, the preparation of B solution: water-soluble binder and 2% suspended dispersed in B solution containing 2% adsorbent and 5%
Agent.Preparation method are as follows: 10g white carbon black, the Aqueous Solutions of Polyethylene Glycol that 125g concentration is 20%, 10g ten are added into deionized water
Dialkyl benzene sulfonic acids sodium is settled to 500mL with deionized water, stirs evenly.
5, the paper prepared in step 3 is immersed in B solution, is sufficiently infiltrated, dried in 90 DEG C.
6, test paper is cut into the strip of 1cm wide, is pasted on the ground not absorbed water, the test-strips of 1cm wide is cut into, that is, makes
At o-phthalaldehyde quick detection test paper card.
The mass percentage content of colored indicator is 5mg in the test card prepared according to the above method.
Embodiment 8: detection of the O-phthalaldehyde disinfectant Concentration Testing test paper to O-phthalic aldehyde concentration in disinfectant
Detection of the Test paper of embodiment 1-7 preparation to O-phthalic aldehyde concentration in disinfectant is carried out as follows,
Wherein disinfectant is solution known to the O-phthalic aldehyde concentration prepared with o-phthalaldehyde standard items.Specific testing conditions and inspection
Survey the results are shown in Table 1.
(1) the embodiment 1-7 Test paper prepared is immersed in solution to be measured known to O-phthalic aldehyde concentration and is carried out
Haptoreaction；
(2) the haptoreaction time takes out the test paper after, removes solution to be measured extra on test paper and (wipes test paper back off
The extra solution to be measured in face)；
(3) being horizontally arranged test paper makes it develop the color naturally (display yellow), compares with standard color comparison card, reads o-phthalaldehyde
Concentration.
Condition and result of 1 Test paper of table to o-phthalaldehyde Concentration Testing in disinfectant
Claims (8)
1. a kind of test paper for detecting o-phthalaldehyde, which is characterized in that the test paper is fixed with colored indicator on plain paper,
The colored indicator is selected from one of amino acid esters compound, arylamine and/or fat primary amine or a variety of combinations, institute
The amino acid esters compound stated is selected from glycine methyl ester, glycine ethyl ester, lysine methyl ester, ethyl ester of lysine, threonine first
Ester, threonine ethyl, tryptophan methyl ester, tryptophan ethyl ester, histidine methylester, histidine ethyl ester, the different monooctyl ester of glycine, glycine
One of ten diester, phenyalanine methyl ester and their salt or a variety of combinations；
The arylamine, fat primary amine are selected from one of open-chain crown ether, dodecyl polyaniline, octyl amine or a variety of groups
It closes；The total amount of the amino acid esters compound, arylamine and/or fat primary amine is 0.2-16mg on test paper described in every.
2. the preparation method of test paper according to claim 1, which comprises the steps of:
(1) it prepares plain paper: preparing the fixative solution of mass volume ratio concentration 0.1%-20%, a certain size filter will be cut into
Paper immerses in the solution, sufficiently after infiltration, dries in 50-90 DEG C；
(2) preparation of solution A: the adsorbent containing mass volume ratio concentration 0.2%-4% in solution A, 0.2%-16%'s is aobvious
Color indicator, the suspension dispersive agent of 0.02%-5%, stirs evenly；
(3) the plain paper that step (1) is prepared immerses in solution A, sufficiently infiltrates, and dries in 50-90 DEG C；
(4) preparation of B solution: the adsorbent containing mass volume ratio concentration 0.2%-6% in B solution, the suspension of 0.1%-6%
Dispersing agent, the adhesive of 0.2%-10%, stirs evenly；
(5) paper that step (3) is prepared immerses in B solution, sufficiently infiltrates, and dries in 50-90 DEG C；
(6) the paper cutouts strip that step (5) is prepared, is pasted on the ground not absorbed water, and is cut into test-strips, that is, is made
At o-phthalaldehyde quick detection test paper.
3. preparation method according to claim 2, it is characterised in that: the fixative is water-soluble high-molecular material,
Selected from one of carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose and their metal salt or a variety of combinations.
4. preparation method according to claim 2, it is characterised in that: the adsorbent is white or colourless absorption
Agent is selected from white carbon black, allkyl methacrylate cross-linked polymer or poly- (lauryl methacrylate -co- glycol dinitrate
Base acrylate) one of or a variety of combinations.
5. preparation method according to claim 2, it is characterised in that: the suspension dispersive agent is anion, nonionic
And/or amphoteric ion dispersing agent, it is selected from iso-octyl benzene sulfonic acid sodium salt, neopelex, alkyl phenol polyoxyethylene ether, propyl
One of glycine betaine or a variety of combinations.
6. preparation method according to claim 2, it is characterised in that: the adhesive is with the water-soluble of bond properties
Property high molecular material, be selected from polyvinyl alcohol and/or polyethylene glycol.
7. the detection method that test paper according to claim 1 is used for O-phthalic aldehyde concentration, which is characterized in that the inspection
Survey method in turn includes the following steps:
(1) test paper is immersed in solution to be measured and carries out haptoreaction；
(2) the haptoreaction time takes out the test paper after, removes solution to be measured extra on test paper；
(3) being horizontally arranged test paper makes it develop the color naturally, compares with standard color comparison card, obtains the concentration of o-phthalaldehyde.
8. detection method according to claim 7, it is characterised in that: the haptoreaction time described in step (1) is 2-
4s；Natural developing time described in step (3) is 1-5min.
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