CN106962188A - Beautiful millettia root seed directly induces the method from bud and fast breeding seedling - Google Patents
Beautiful millettia root seed directly induces the method from bud and fast breeding seedling Download PDFInfo
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- CN106962188A CN106962188A CN201710118967.3A CN201710118967A CN106962188A CN 106962188 A CN106962188 A CN 106962188A CN 201710118967 A CN201710118967 A CN 201710118967A CN 106962188 A CN106962188 A CN 106962188A
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- culture
- bud
- seedling
- beautiful millettia
- fast breeding
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The method from bud and fast breeding seedling is directly induced the present invention relates to beautiful millettia root seed, culture medium, Initial culture is prepared, from fast breeding culture outside bud Multiplying culture, further strong seedling culture, bottle.The seed that the present invention is provided directly induction is from the method for bud and fast breeding seedling, and healthy and strong with the neat, seedling that sprouts, nursery success rate is high, and seedling raise period is short, the low advantage of seedling cost.
Description
Technical field
The invention belongs to field of plant growing technology, and in particular to beautiful millettia root seed is directly induced from bud and fast breeding kind
The method of seedling.
Background technology
Beautiful millettia root is the root tuber of Millettia beautiful millettia, with stimulating the circulation of the blood and cause the muscles and joints to relax, clearing and antitussive, the work(such as moistening lung nourshing kidney
Effect, is distributed in Guangxi, Guangdong, Hainan, Taiwan, Guizhou and Southeast Asia, is that a kind of medicine-food two-purpose with good health-care efficacy is planted
Thing.In recent years, due to a variety of Chinese patent drugs such as the loins-strengthening and kidney-invigorating bolus using beautiful millettia root as main material production, building-up body capsules,
In the market sales volume is continuously increased, and causes also to sharply increase the demand of its raw material, and wild beautiful millettia root medicinal material is into endangered breed, people
Work post, which plants beautiful millettia root, turns into inexorable trend.At present, about 30,000 mu of beautiful millettia root cultivated area, based on every mu of young plant of kind 600, it is necessary to
18000000 plants of seedling.It is inconsistent with batch kernel maturing of harvesting because the florescence of beautiful millettia root and fruiting period are longer, cause ox big
Power germination is extremely irregular, there is what is germinateed in one month, also there is what is germinateed after half a year, and germination percentage is relatively low, average in 42.5-
Between 61.25%.
Compared with beautiful millettia root tissue-cultured seedling and cuttage seeding, the seedling of breeding is planted with beautiful millettia root, growth is quick, plant last year
The existing finger of diameter is big, that is, forms the main root tuber (potato wedge) expanded, the optimal selection of seed shootses artificial growth beautiful millettia root.But
Beautiful millettia root seed seedling-raising is all small-scale progress on the market at present, adds percentage of seedgermination low irregular with germination, it is difficult to meet
The extensive demand in market.Therefore, the present invention has important practical significance to the market demand for meeting beautiful millettia root seed shootses.
The content of the invention
It is an object of the invention to solve at least the above and/or defect, and provide at least will be described later excellent
Point.
The method from bud and fast breeding seedling is directly induced it is a still further object of the present invention to provide beautiful millettia root seed, should
Method can realize beautiful millettia root seed scale breeding, once can the strain of nursery hundreds of thousands arrive millions of strains, and seedling is neat, seed
Germination percentage is more than 97%, and survival rate is more than 98%, and the total success rate of nursery is more than 95%, and seedling raise period can shift to an earlier date 1-2
Month.
Directly induced to realize according to object of the present invention and further advantage there is provided beautiful millettia root seed from bud simultaneously
The method of fast breeding seedling, including,
The step of Initial culture, is as follows：Clip beautiful millettia root semi-lignified stem bar, removes blade, is cut into 3-5cm long stem section,
Rinsed well with water, after ultrasonic sterilization 7-10min, with aseptic water washing 5-6 times, wipe out two ends otch, be cut into 1-1.5em length
Stem with bud, be inoculated into after Initial culture base, light culture 7-8d, axillary bud is sprouted, then dim light culture 25d, axillary bud grows to 1cm,
Progress culture 30d under strong light is transferred to, axillary bud grows up to the single sprout of 1.5-3cm length, on-bladed differentiation.
From bud Multiplying culture the step of it is as follows：The sprout stem that Initial culture grows up to is cut into 1.5-2cm long stem section traverse
It is linked into from bud proliferated culture medium, by 28d culture, 5-7 Multiple Buds is sprouted at each stem segment with axillary buds, budlet length are treated extremely
3-6cm, gradually differentiates 1-3 piece compound leaves.
The step of strong seedling culture, is as follows：The high about 4cm of clip, the sprout individual plant for having 2 compound leaves, which is placed vertically, is transferred to strong seedling culture
5-6d is cultivated under base, dim light, is transferred under strong light and cultivates；Cultivate 16-20d, height of seedling 4-5cm, leaf dark green, stem degree of lignification
Height, grows vigorous.
The outer quick numerous step of bottle is as follows：Culture transplanting medium is educated by yellow soil: river sand: peat soil (10-15: 1: 1)
Ratio is mixed thoroughly, is installed with 8cm × 12cm nutrient bags standby；Transplanting the previous day is disappeared with 0.1%-0.2% liquor potassic permanganates
Poison, drenches matrix with clear water during transplanting；Before transplanting, strong sprout in bottle is cleaned into base portion culture medium with clear water, then be placed in 500mg/L
About 1min is dipped in ABT root-inducing powders, Small plastic shed plastic covering film and black net are taken in cuttage in nutrition bag as substrate, immediately, kept
Relative humidity is maintained at more than 90% in seedbed epidemic disaster, canopy, and temperature control after transplanting 1 week, can open portion below 30 DEG C
Divide film breathable, the area of film is opened later to be gradually increased, 1 bactericide and foliar fertilizer are sprayed at interval of 7d, transplant 40d
Go out 2, main root, rooting rate reaches 75%-85%；Rooted seedling is up to 10cm after 90d, and main root 1-4 bars, majority is 2, and rugosity reaches
0.15cm, root length is most long up to 15cm, fibrous root 10-20 bars；After 180d, main root enlarges into cylindric or two spindles and is linked to be one
String, expands place's diameter and reaches 0.8-1.3cm.
Preferably, the culture medium is MS culture mediums, and the culture medium adds sucrose 3%, pH value 5.6-5.8, culture
Temperature (26 ± 1) DEG C, light application time 12h/d, intensity of illumination 400-20001x；Added in the Initial culture base by expediting the emergence of element.
The present invention at least includes following beneficial effect：
Firstth, the method that provides of the present invention, realizes the directly induction of beautiful millettia root seed from bud and fast breeding seedling, once
Can the strain of nursery hundreds of thousands arrive millions of strains, and seedling is neat, percentage of seedgermination more than 97%, survival rate more than 98%,
The total success rate of nursery is more than 95%, and seedling raise period can shift to an earlier date 1-2 months, solve beautiful millettia root germination it is irregular, can not
Scale breeding and the low problem of germination percentage；
Secondth, by Optimal Medium and from bud Multiplying culture, material in beautiful millettia root tissue culture procedures can effectively be prevented
Browning and abnormal callus are produced, and compound leaf can be promoted to break up, and the degree of lignification of increase Regenerated plant height and stem, is outside bottle
Fast breeding provides high-quality effective sprout.
Other advantages, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to specification
Word can be implemented according to this.
Experimental method described in following embodiments, is conventional method unless otherwise specified, the reagent and material, such as
Without specified otherwise, commercially obtain.
Embodiment：
Beautiful millettia root seed directly induces the method from bud and fast breeding seedling, including,
The step of Initial culture, is as follows：Clip beautiful millettia root semi-lignified stem bar, removes blade, is cut into 4cm long stem section, uses
Water is rinsed well, after ultrasonic sterilization 8min, with aseptic water washing 5 times, is wiped out two ends otch, is cut into the stem with bud of 1cm length,
It is inoculated into after Initial culture base, light culture 7d, axillary bud is sprouted, then dim light culture 25d, axillary bud grows to 1cm, is transferred under strong light
Row culture 30d, axillary bud grows up to the single sprout of 1.5cm length, on-bladed differentiation.
From bud Multiplying culture the step of it is as follows：The sprout stem that Initial culture grows up to is cut into 1.5 long stem section traverse access
To from bud proliferated culture medium, by 28d culture, 5 Multiple Buds are sprouted at each stem segment with axillary buds, budlet length are treated to 3cm, by
Gradually differentiate 2 compound leaves.
The step of strong seedling culture, is as follows：The high about 4cm of clip, the sprout individual plant for having 2 compound leaves, which is placed vertically, is transferred to strong seedling culture
5d is cultivated under base, dim light, is transferred under strong light and cultivates；16d is cultivated, height of seedling 4cm, leaf dark green, stem degree of lignification is high, growth
It is vigorous.
The outer quick numerous step of bottle is as follows：Culture transplanting medium is educated by yellow soil：River sand：The ratio of peat soil (10: 1: 1)
Mix thoroughly, installed with 8cm × 12cm nutrient bags standby；Transplanting the previous day is carried out disinfection with 0.1% liquor potassic permanganate, is used during transplanting
Clear water drenches matrix；Before transplanting, strong sprout in bottle is cleaned into base portion culture medium with clear water, then be placed in 500mg/L ABT root-inducing powders
In dip about 1min, Small plastic shed plastic covering film and black net are taken in cuttage in nutrition bag as substrate, immediately, keep seedbed temperature, wet
Relative humidity is maintained at more than 90% in degree, canopy, and temperature control after transplanting 1 week, can open part film breathable at 27 DEG C, with
Opening the area of film afterwards can be gradually increased, and 1 bactericide and foliar fertilizer are sprayed at interval of 7d, transplanted 40d and gone out 2, main root, raw
Root rate is up to 75%；Rooted seedling is up to 10cm, 2, main root after 90d, and rugosity reaches 0.15cm, and root length is most long up to 15cm, fibrous root 15
Bar；After 180d, main root enlarges into cylindric or two spindles and strung, and expands place's diameter and reaches 1.3cm.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With.It can be applied to various suitable the field of the invention completely., can be easily for those skilled in the art
Realize other modification.Therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details and shown here as the example with description.
Claims (6)
1. beautiful millettia root seed directly induces the method from bud and fast breeding seedling, it is characterised in that comprise the following steps：Prepare
Culture medium, Initial culture, from fast breeding culture outside bud Multiplying culture, further strong seedling culture, bottle.
2. beautiful millettia root seed according to claim 1 directly induces the method from bud and fast breeding seedling, its feature exists
It is MS culture mediums in, the culture medium, the culture medium adds sucrose 3%, pH value 5.6-5.8, cultivation temperature (26 ± 1) DEG C,
Light application time 12h/d, intensity of illumination 400-2000lx；It is added with the Initial culture base and expedites the emergence of element.
3. beautiful millettia root seed according to claim 1 directly induces the method from bud and fast breeding seedling, its feature exists
It is as follows the step of, the Initial culture：Clip beautiful millettia root semi-lignified stem bar, removes blade, is cut into 3-5cm long stem section,
Rinsed well with water, after ultrasonic sterilization 7-10min, with aseptic water washing 5-6 times, wipe out two ends otch, be cut into 1-1.5cm length
Stem with bud, be inoculated into after Initial culture base, light culture 7-8d, axillary bud is sprouted, then dim light culture 25d, axillary bud grows to 1cm,
Progress culture 30d under strong light is transferred to, axillary bud grows up to the single sprout of 1.5-3cm length, on-bladed differentiation.
4. beautiful millettia root seed according to claim 1 directly induces the method from bud and fast breeding seedling, its feature exists
It is as follows the step of, the Multiplying culture from bud：The long stem section traverse that the sprout stem that Initial culture grows up to is cut into 1.5-2cm connects
Enter to from bud proliferated culture medium, by 28d culture, 5-7 Multiple Buds are sprouted at each stem segment with axillary buds, treat budlet length to 3-
6cm, gradually differentiates 1-3 piece compound leaves.
5. beautiful millettia root seed according to claim 1 directly induces the method from bud and fast breeding seedling, its feature exists
It is as follows the step of, the strong seedling culture：The high about 4cm of clip, the sprout individual plant for having 2 compound leaves, which is placed vertically, is transferred to strong seedling culture
5-6d is cultivated under base, dim light, is transferred under strong light and cultivates；Cultivate 16-20d, height of seedling 4-5cm, leaf dark green, stem degree of lignification
Height, grows vigorous.
6. beautiful millettia root seed according to claim 1 directly induces the method from bud and fast breeding seedling, its feature exists
In quick numerous step is as follows outside the bottle：Culture transplanting medium is educated by yellow soil: river sand: the ratio of peat soil (10-15: 1: 1)
Example is mixed thoroughly, is installed with 8cm × 12cm nutrient bags standby；Transplanting the previous day is carried out disinfection with 0.1%-0.2% liquor potassic permanganates,
Matrix is drenched with clear water during transplanting；Before transplanting, strong sprout in bottle is cleaned into base portion culture medium with clear water, then be placed in 500mg/L
About 1min is dipped in ABT root-inducing powders, Small plastic shed plastic covering film and black net are taken in cuttage in nutrition bag as substrate, immediately, kept
Relative humidity is maintained at more than 90% in seedbed epidemic disaster, canopy, and temperature control after transplanting 1 week, can open portion below 30 DEG C
Divide film breathable, the area of film is opened later to be gradually increased, 1 bactericide and foliar fertilizer are sprayed at interval of 7d, transplant 40d
Go out 2, main root, rooting rate reaches 75%-85%；Rooted seedling is up to 10cm after 90d, and main root 1-4 bars, majority is 2, and rugosity reaches
0.15cm, root length is most long up to 15cm, fibrous root 10-20 bars；After 180d, main root enlarges into cylindric or two spindles and is linked to be one
String, expands place's diameter and reaches 0.8-1.3cm.
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| CN201710118967.3A CN106962188A (en) | 2017-03-01 | 2017-03-01 | Beautiful millettia root seed directly induces the method from bud and fast breeding seedling |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108668814A (en) * | 2018-05-03 | 2018-10-19 | 梁秀芳 | Beautiful millettia root root system cross growth method |
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Application publication date: 20170721 |