CN101379950A - Method for quickly breeding millettia speciosa champ germchit using test tube micro cuttage - Google Patents
Method for quickly breeding millettia speciosa champ germchit using test tube micro cuttage Download PDFInfo
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- CN101379950A CN101379950A CNA2008101728561A CN200810172856A CN101379950A CN 101379950 A CN101379950 A CN 101379950A CN A2008101728561 A CNA2008101728561 A CN A2008101728561A CN 200810172856 A CN200810172856 A CN 200810172856A CN 101379950 A CN101379950 A CN 101379950A
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Abstract
The invention discloses a method for rapid propagation of beautiful millettia root germchits by using test tube micro-cuttage, which comprises: beautiful millettia root seeds are inoculated into the culture medium for germination and cultivated until the seeds grow into seedlings; the seedlings obtained are cut into stem sections, inserted into the micro-cuttage culture medium and cultivated until the lateral buds come out, then, the lateral buds are cut into stem sections and inserted into a fresh micro-cuttage culture medium for cultivation; after being cut into stem sections, the lateral buds obtained are inoculated into a rooting medium and cultivated until the stem sections take root, thereby obtaining the beautiful millettia root germchits. The technique is simple and easy, has the advantages of simplicity in operation and high propagation rate, solves the problems of small seeds number, low germinating capacity and low propagation rate of germchits propagation by using beautiful millettia root seeds, and realizes long-term large-scale rapid propagation of beautiful millettia root germchits. The technique can obtain high quality beautiful millettia root test tube sprouts in large quantity quickly and continuously, and has strong practicability.
Description
Technical field
The invention belongs to agricultural biological technical field, be specially a kind of method of utilizing test tube stem section micro cuttage to breed the Niu Dali seedling fast.
Background technology
Ox belongs to the Papilionaceae reticulate millettia energetically and belongs to, and the beginning is stated from " the sward property of medicine is wanted fully ", and function is the qi-restoratives moistening lung, and strong muscle is active.Be used for lumbar muscle strain, rheumatic arthritis is controlled lung heat, cough due to deficiency of the lung, pulmonary tuberculosis, chronic bronchitis, chronic hepatitis, seminal emission, leukorrhea etc.Begin the seventies as the raw material of loins-strengthening and kidney-invigorating bolus, building-up body capsule etc. and be used for the production of Chinese patent drug, be used widely in the area, Guangdong and Guangxi Provinces; Last century Mo this begin, the ground Niu Dali that generally use among the people such as Guangdong, Hong Kong, Macao boil beautiful soup, consumption is considerably beyond pharmacy corporation, and the gesture of growth is year by year arranged.Ox is used as medicine with root energetically, generally takes the cylindric or spindle shape piece root supply the market of the Niu Dali in 4～5 years of growth in the fall.Because Niu Dali is all from wild resource, constantly coyoting is dug excessively, and resource is endangered energetically to make wild ox.
The traditional energetically propagation method of ox is seminal propagation, and the problem that this method exists is: (1) seed resource is in short supply, gathers difficulty in batches; (2) seed longevity is short, can not low-temperature storage, and therefore regular seeding and seedling raising; (3) percentage of seedgermination is low, only reaches about 45%; (4) seed shoot root system is based on main root, and it is few that the piece root forms quantity, yields poorly.
In recent years, cottage propagation and tissue culture propagation became the research emphasis of Niu Dali sapling multiplication.The existing problem of cottage propagation is: (1) cottage propagation survival rate is low, even all dead; (2) cutting resource scarcity, breeding difficulty in batches.Tissue culture propagation adopts the mode of callus induction, callus differentiation and proliferation and adventitious bud rooting to obtain aseptic seedling at present, and the problem that this mode exists is: (1) evoked callus process time is longer; (2) to be divided into the frequency of indefinite bud lower for callus, and the indefinite bud variation rate that differentiates improves; (3) the indefinite bud elongation speed that differentiates of callus is slow, and the time that reaches the standard of taking root is long.These problems have seriously hindered the large-scale production of Niu Dali seedling, thereby can't satisfy market to ox demand energetically.For this reason, must foundation can expand numerous ox production technology system of seedling energetically in a large number, fast.
Ox belongs to the Papilionaceae reticulate millettia energetically and belongs to, and the beginning is stated from " the sward property of medicine is wanted fully ", and function is the qi-restoratives moistening lung, and strong muscle is active.Be used for lumbar muscle strain, rheumatic arthritis is controlled lung heat, cough due to deficiency of the lung, pulmonary tuberculosis, chronic bronchitis, chronic hepatitis, seminal emission, leukorrhea etc.Begin the seventies as the raw material of loins-strengthening and kidney-invigorating bolus, building-up body capsule etc. and be used for the production of Chinese patent drug, be used widely in the area, Guangdong and Guangxi Provinces; Last century Mo this begin, the ground Niu Dali that generally use among the people such as Guangdong, Hong Kong, Macao boil beautiful soup, consumption is considerably beyond pharmacy corporation, and the gesture of growth is year by year arranged.Ox is used as medicine with root energetically, generally takes the cylindric or spindle shape piece root supply the market of the Niu Dali in 4～5 years of growth in the fall.Because Niu Dali is all from wild resource, constantly coyoting is dug excessively, and resource is endangered energetically to make wild ox.
The traditional energetically propagation method of ox is seminal propagation, and the problem that this method exists is: (1) seed resource is in short supply, gathers difficulty in batches; (2) seed longevity is short, can not low-temperature storage, and therefore regular seeding and seedling raising; (3) percentage of seedgermination is low, only reaches about 45%; (4) seed shoot root system is based on main root, and it is few that the piece root forms quantity, yields poorly.
In recent years, cottage propagation and tissue culture propagation became the research emphasis of Niu Dali sapling multiplication.The existing problem of cottage propagation is: (1) cottage propagation survival rate is low, even all dead; (2) cutting resource scarcity, breeding difficulty in batches.Tissue culture propagation adopts the mode of callus induction, callus differentiation and proliferation and adventitious bud rooting to obtain aseptic seedling at present, and the problem that this mode exists is: (1) evoked callus process time is longer; (2) to be divided into the frequency of indefinite bud lower for callus, and the indefinite bud variation rate that differentiates improves; (3) the indefinite bud elongation speed that differentiates of callus is slow, and the time that reaches the standard of taking root is long.These problems have seriously hindered the large-scale production of Niu Dali seedling, thereby can't satisfy market to ox demand energetically.
Summary of the invention
The purpose of this invention is to provide a kind of method of utilizing the test tube micro cuttage to breed the Niu Dali seedling fast, is a kind of utilize stem section sprouting of lateral bud and fireballing characteristics of elongation in the test tube, has realized the numerous purpose of the extensive expansion of Niu Dali seedling.
The present invention utilizes small quantities of seed to obtain aseptic seedling, is explant with the aseptic seedling stem sections, constantly sprouts by test tube micro cuttage promotion lateral bud and carries out fast breeding, realizes that the extensive expansion of Niu Dali seedling is numerous.
The technical solution adopted in the present invention: a kind of method of utilizing the test tube micro cuttage to breed the Niu Dali seedling fast comprises procurement process, stem section micro cuttage process, the stem section rooting process of aseptic seedling.
1, the procurement process of aseptic seedling
The pod of choosing Niu Dali strips out seed after sterilization, seed is inoculated into the germination medium is cultured to and grows up to seedling, and described germination medium is to be minimal medium and additional 6-benzyladenine, α-Nai Yisuan with the MS medium.
2, stem section micro cuttage process
The seedling that step 1 obtained is cut into the stem section, the base portion of stem section is inserted downwards to be cultured in the micro cuttage medium grows lateral bud, again lateral bud is cut into the stem section, its base portion is inserted in the fresh micro cuttage medium downwards cultivate then.Described micro cuttage medium is to be minimal medium and to add 6-benzyladenine, heteroauxin with the MS medium.
3, stem section rooting process
The lateral bud that step 2 obtained is cut into the stem section, and it is inoculated on the root media, illumination cultivation is carried out in earlier dark cultivation again, and taking root to the stem section to obtain the Niu Dali seedling.Described root media is to be minimal medium and additional peptone, IAA, IBA, vitamin h with the 1/2MS medium.
The present invention is simple for process; simple to operate; growth rate is fast; solved and utilized in the Niu Dali seminal propagation seedling seed amount few; germination rate is low; the problem that reproduction speed is slow; realized the long-term extensive fast breeding of Niu Dali seedling; can be a large amount of; fast; continue to obtain high-quality ox test-tube plantlet energetically; practical; expand numerous propagation for Niu Dali is extensive high-efficiency method is provided; scale for the Niu Dali seedling; batch production is produced and is laid a good foundation; for stablize ox energetically market supply guarantee is provided, also for the protection wild ox energetically germplasm opened up a practicable approach.
Embodiment
Below the invention will be further described.
The method of utilizing the test tube micro cuttage to breed the Niu Dali seedling fast provided by the present invention comprises procurement process, stem section micro cuttage process, the stem section rooting process of aseptic seedling.
1), the procurement process of aseptic seedling
The pod of choosing Niu Dali strips out seed after sterilization, seed is inoculated into the germination medium is cultured to and grows up to seedling, and the cultivation temperature degree is 25～28 ℃, and intensity of illumination is 15～20 μ molm
-2S
-1, light application time is 8hd
-1, cultivated 40～60 days; Described germination medium is to be minimal medium and to add 6-benzyladenine 0.5mgL with the MS medium
-1, α-Nai Yisuan 0.1mgL
-1
2), stem section micro cuttage process
The seedling that step 1 obtained is cut into the stem section that saves with 1～2, the base portion of stem section inserted downwards to be cultured in the micro cuttage medium grow lateral bud, again lateral bud is cut into stem section with 1～2 joint, then its base portion is inserted in the fresh micro cuttage medium downwards and cultivate, the cultivation temperature degree is 27～29 ℃, and intensity of illumination is 30～40 μ molm
-2S
-1, light application time is 8hd
-1Described micro cuttage medium is to be minimal medium and to add 6-benzyladenine 2.0mgL with the MS medium
-1, heteroauxin 0.1mgL
-1
3), stem section rooting process
The lateral bud that step 2 obtained is cut into the stem section that saves with 1～2, and it is inoculated on the root media, dark earlier the cultivation carried out illumination cultivation 20～30 days in 8～13 days again, and taking root to the stem section to obtain the Niu Dali seedling.Described root media is to be minimal medium and additional peptone 1.0gL with the 1/2MS medium
-1, IAA 0.8mgL
-1, IBA 0.5mgL
-1, vitamin h 0.5mgL
-1
Claims (1)
1, a kind of method of utilizing the test tube micro cuttage to breed the Niu Dali seedling fast is characterized in that: the procurement process, stem section micro cuttage process, the stem section rooting process that comprise aseptic seedling;
1), the procurement process of aseptic seedling
The pod of choosing Niu Dali strips out seed after sterilization, seed is inoculated into the germination medium is cultured to and grows up to seedling, and the cultivation temperature degree is 25～28 ℃, and intensity of illumination is 15～20 μ molm
-2S
-1, light application time is 8hd
-1, cultivated 40～60 days; Described germination medium is to be minimal medium and to add 6-benzyladenine 0.5mgL with the MS medium
-1, α-Nai Yisuan 0.1mgL
-1
2), stem section micro cuttage process
The seedling that step 1 obtained is cut into the stem section that saves with 1～2, the base portion of stem section inserted downwards to be cultured in the micro cuttage medium grow lateral bud, again lateral bud is cut into stem section with 1～2 joint, then its base portion is inserted in the fresh micro cuttage medium downwards and cultivate, the cultivation temperature degree is 27～29 ℃, and intensity of illumination is 30～40 μ molm
-2S
-1, light application time is 8hd
-1Described micro cuttage medium is to be minimal medium and to add 6-benzyladenine 2.0mgL with the MS medium
-1, heteroauxin 0.1mgL
-1
3), stem section rooting process
The lateral bud that step 2 obtained is cut into the stem section that saves with 1～2, and it is inoculated on the root media, dark earlier the cultivation carried out illumination cultivation 20～30 days in 8～13 days again, and taking root to the stem section to obtain the Niu Dali seedling.Described root media is to be minimal medium and additional peptone 1.0gL with the 1/2MS medium
-1, IAA0.8mgL
-1, IBA0.5mgL
-1, vitamin h 0.5mgL
-1
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102893870A (en) * | 2012-10-09 | 2013-01-30 | 冯志祥 | Rapid propagation and seedling raising method for beautiful millettia root seedling tissue culture |
| CN103039361A (en) * | 2013-01-11 | 2013-04-17 | 广西壮族自治区药用植物园 | Method for directly inducing cluster buds of milletia speciosa champ seeds and quickly breeding seedlings |
| CN104429970A (en) * | 2014-12-18 | 2015-03-25 | 曾雷 | Rooting culture medium inmillettia fordii dunn tissue culture rooting method and millettia fordii dunn tissue culture rooting method |
| CN104521764A (en) * | 2015-01-26 | 2015-04-22 | 广西壮族自治区药用植物园 | Method for achieving tuberization of beautiful millettia root tissue culture seedling in bottle |
| CN105123515A (en) * | 2015-07-28 | 2015-12-09 | 河北农业大学 | Method for preserving segregation population or distant hybirdization progeny of eggplant through stem microcuttage |
| CN106386511A (en) * | 2016-12-07 | 2017-02-15 | 广西壮族自治区药用植物园 | Rapid propagation method for suspension cell culture of Millettia speciosa Champ |
| CN106688893A (en) * | 2017-01-15 | 2017-05-24 | 黄振忠 | Beautiful millettia root tissue culture seedling raising method |
| CN106962188A (en) * | 2017-03-01 | 2017-07-21 | 防城港市防城区蚕种场 | Beautiful millettia root seed directly induces the method from bud and fast breeding seedling |
| CN107135944A (en) * | 2017-05-04 | 2017-09-08 | 南宁市辉雄中药材农民专业合作社 | A kind of beautiful millettia root root tissues culture medium and preparation method thereof |
| CN108886987A (en) * | 2018-06-11 | 2018-11-27 | 中国科学院昆明植物研究所 | A kind of castor-oil plant lateral bud Rapid Rooting propagation method |
| CN109258152A (en) * | 2018-11-30 | 2019-01-25 | 泰安市泰山林业科学研究院 | Micro- stem cuttage breeding method in a kind of Mount Taishan Asian puccoon bottle |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101124891A (en) * | 2006-08-15 | 2008-02-20 | 钦州市中医药研究所 | Method for propagating tissue cultured millettia specisoa champ seedling |
-
2008
- 2008-10-24 CN CN2008101728561A patent/CN101379950B/en not_active Expired - Fee Related
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102893870A (en) * | 2012-10-09 | 2013-01-30 | 冯志祥 | Rapid propagation and seedling raising method for beautiful millettia root seedling tissue culture |
| CN102893870B (en) * | 2012-10-09 | 2014-03-12 | 冯志祥 | Rapid propagation and seedling raising method for beautiful millettia root seedling tissue culture |
| CN103039361A (en) * | 2013-01-11 | 2013-04-17 | 广西壮族自治区药用植物园 | Method for directly inducing cluster buds of milletia speciosa champ seeds and quickly breeding seedlings |
| CN104429970A (en) * | 2014-12-18 | 2015-03-25 | 曾雷 | Rooting culture medium inmillettia fordii dunn tissue culture rooting method and millettia fordii dunn tissue culture rooting method |
| CN104521764B (en) * | 2015-01-26 | 2016-07-06 | 广西壮族自治区药用植物园 | A kind of method of knot potato in Millettia Speciosa tissue cultured seedling bottle |
| CN104521764A (en) * | 2015-01-26 | 2015-04-22 | 广西壮族自治区药用植物园 | Method for achieving tuberization of beautiful millettia root tissue culture seedling in bottle |
| CN105123515A (en) * | 2015-07-28 | 2015-12-09 | 河北农业大学 | Method for preserving segregation population or distant hybirdization progeny of eggplant through stem microcuttage |
| CN106386511A (en) * | 2016-12-07 | 2017-02-15 | 广西壮族自治区药用植物园 | Rapid propagation method for suspension cell culture of Millettia speciosa Champ |
| CN106386511B (en) * | 2016-12-07 | 2018-05-29 | 广西壮族自治区药用植物园 | The rapid propagation method of beautiful millettia root suspension cell culture |
| CN106688893A (en) * | 2017-01-15 | 2017-05-24 | 黄振忠 | Beautiful millettia root tissue culture seedling raising method |
| CN106962188A (en) * | 2017-03-01 | 2017-07-21 | 防城港市防城区蚕种场 | Beautiful millettia root seed directly induces the method from bud and fast breeding seedling |
| CN107135944A (en) * | 2017-05-04 | 2017-09-08 | 南宁市辉雄中药材农民专业合作社 | A kind of beautiful millettia root root tissues culture medium and preparation method thereof |
| CN108886987A (en) * | 2018-06-11 | 2018-11-27 | 中国科学院昆明植物研究所 | A kind of castor-oil plant lateral bud Rapid Rooting propagation method |
| CN109258152A (en) * | 2018-11-30 | 2019-01-25 | 泰安市泰山林业科学研究院 | Micro- stem cuttage breeding method in a kind of Mount Taishan Asian puccoon bottle |
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| CN101379950B (en) | 2010-12-08 |
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