CN106929457A - A kind of Ke Liben series bacillus granule and preparation method thereof - Google Patents
A kind of Ke Liben series bacillus granule and preparation method thereof Download PDFInfo
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- CN106929457A CN106929457A CN201710323746.XA CN201710323746A CN106929457A CN 106929457 A CN106929457 A CN 106929457A CN 201710323746 A CN201710323746 A CN 201710323746A CN 106929457 A CN106929457 A CN 106929457A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The present invention relates to a kind of Ke Liben series bacillus granule and preparation method thereof, belong to technical field of pesticide.The present invention provide Ke Liben series bacillus granules, including following parts by weight component：4 parts of Ke Liben series bacillus；13~18 parts of auxiliary agent；55~68 parts of filler；18~20 parts of water.The Ke Liben series bacillus granule bioactivity for providing of the invention is high, good antimicrobial effect, and is easy to transport and stores.
Description
Technical field
The present invention relates to technical field of pesticide, more particularly to a kind of Ke Liben series bacillus granule and its preparation side
Method.
Background technology
Ke Liben series bacillus belong to Bacillaceae, and one kind of bacillus genus, the bacterium broad spectrum activity is good, antibacterial work
Property it is high, have prevention effect to plurality of plant diseases, in addition the sterilization plant fermentation process in produce secondary metabolites poly really
Sugar also has growth-promoting characteristic to plant, while the bacterial strain also has phosphate solubilization, with market value very high.
Conventional is Ke Liben class gemma bar aquas at present, and inconvenience is transported, packing cost is big, be difficult the factor of storage, raw
Thing activity holding effect is short, and drug effect is affected so that it has certain regionality in popularization and use.
The content of the invention
It is an object of the invention to provide a kind of Ke Liben series bacillus granule.The Ke Liben class buds that the present invention is provided
Spore bacillus granule bioactivity is high, good antimicrobial effect, and is easy to transport and stores.
The invention provides a kind of Ke Liben series bacillus granule, including following parts by weight component：
4 parts of Ke Liben series bacillus；
13~18 parts of auxiliary agent；
55~68 parts of filler；
18~20 parts of water.
Preferably, the biological deposits number of described Ke Liben class bacillus strains are CGMCC No.7996.
Preferably, the auxiliary agent includes white granulated sugar and cornstarch, and the mass ratio of the white granulated sugar and cornstarch is
1：(4~7.5).
Preferably, described filler includes talcum powder and kaolin；The talcum powder and kaolinic mass ratio for (1~
2.5)：1.
Preferably, the content of Ke Liben class bacillus is 190~210IU/mg in the granule.
Preferably, loss on drying≤6% of the granule.
Preferably, the pH value of the granule is 6~8.
Preferably, suspensibility >=80% of the granule.
Present invention also offers the preparation method of granule described in above-mentioned technical proposal, comprise the following steps：By Ke Liben
Stirred after the mixing of series bacillus, auxiliary agent, filler and water, carry out air-flow crushing, obtain granule.
Obtained present invention also offers preparation method described in granule described in above-mentioned technical proposal or above-mentioned technical proposal
Application of the granule in rice sheath blight disease is prevented and treated.
The invention provides a kind of Ke Liben series bacillus granule, including following parts by weight component：Ke Liben
4 parts of series bacillus；13~18 parts of auxiliary agent；55~68 parts of filler and water.The Ke Liben series bacillus particles that the present invention is provided
Agent bioactivity is high, good antimicrobial effect, and is easy to transport and stores.
Biological deposits explanation
Ke Liben series bacillus (Paenibacillus kribbensis), China was deposited on 08 13rd, 2013
Microbiological Culture Collection administration committee common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, in
Institute of microbiology of the academy of sciences of state, biological deposits numbering is CGMCC No.7996.
Brief description of the drawings
Fig. 1 is the Ke Liben series bacillus antimicrobial spectrums that the embodiment of the present invention 3 is provided.
Specific embodiment
The invention provides a kind of Ke Liben series bacillus granule, including following parts by weight component：
4 parts of Ke Liben series bacillus；
13~18 parts of auxiliary agent；
55~68 parts of filler；
18~20 parts of water.
In the present invention, the Ke Liben series bacillus granule includes 4 parts of Ke Liben class bacillus.The present invention
The culture presevation of the Ke Liben series bacillus is raw in China Committee for Culture Collection of Microorganisms's common micro-organisms center
Thing deposit number is CGMCC No.7996.The present invention does not have special limit to the preparation method of the Ke Liben classes bacillus
It is fixed, using the preparation method of Ke Liben classes bacillus well known to those skilled in the art.In the present invention, the particle
The content of Ke Liben classes bacillus is 190~210IU/mg, more preferably 200IU/mg in agent.
On the basis of 4 parts of Ke Liben class bacillus, the Ke Liben series bacillus granule bags that the present invention is provided
Include 13~18 parts of auxiliary agent, preferably 15 parts.In the present invention, the auxiliary agent preferably includes white granulated sugar and cornstarch, described
The mass ratio of white granulated sugar and cornstarch is preferably 1：(4~7.5), more preferably 1：5.In the present invention, the white granulated sugar rises
To the effect of binding agent, the cornstarch belongs to disintegrant, accelerates into water dilution rate.The present invention is to the white granulated sugar and jade
Without special restriction, the commercially available prod using white sand sugar and starch well known to those skilled in the art is in the source of rice starch
Can.
On the basis of 4 parts of Ke Liben class bacillus, the Ke Liben series bacillus granule bags that the present invention is provided
Include 55~68 parts of filler, preferably 60 parts.In the present invention, described filler preferably includes talcum powder and kaolin；The cunning
Stone flour and kaolinic mass ratio are preferably (1~2.5)：1.In the present invention, the talcum powder plays lubrication, accelerates to make
Go out a speed during grain；The kaolin belongs to inserts, plays diluting effect.It is of the invention to the talcum powder and kaolinic
Originate without special restriction, using talcum powder well known to those skilled in the art and kaolinic conventional commercial product.
In the present invention, loss on drying≤6% of the granule.
In the present invention, the pH value of the granule is 6~8.
In the present invention, suspensibility >=80% of the granule.
In the present invention, the particle diameter of the granule is 1.0~1.2mm.
Present invention also offers the preparation method of granule described in above-mentioned technical proposal, comprise the following steps：By Ke Liben
Stirred after the mixing of series bacillus, auxiliary agent, filler and water, carry out air-flow crushing, obtain granule.
In the present invention, the time of the stirring be preferably 0.8~2h, more preferably 1h.
The present invention does not have special restriction to the device of air-flow crushing, using air-flow crushing well known to those skilled in the art
Machine.Such as QYF-60 airslide disintegrating mills.Specifically, in specific Crushing with Jet Mill, sealing pressure is preferably 0.1~
0.15Mpa, more preferably 0.12Mpa；Discharging sealing gland pressure is preferably 0.15Mpa；Pulse is preferably 0.4~0.6Mpa,
More preferably 0.5Mpa；Feed pressure is preferably 0.75~0.85Mpa, more preferably 0.8Mpa；Dnockout speed is crushed to be preferably
200~300kg/h, more preferably 240kg/h；Crush inventory and be preferably 800~1000kg/ batches, more preferably 850kg/ batches；
Mediate water rate control and be preferably 20~25%, more preferably 23%；Prilling temperature control is preferably 50~60 DEG C, more preferably 55
℃。
Obtained present invention also offers preparation method described in granule described in above-mentioned technical proposal or above-mentioned technical proposal
Application of the granule in rice sheath blight disease is prevented and treated.
A kind of Ke Liben series bacillus granule provided the present invention with reference to specific embodiment and its preparation side
Method is further described in detail, and technical scheme includes but is not limited to following examples.
Embodiment 1
The preparation of Ke Liben series bacillus primary seed solutions：The Ke Liben series bacillus strains of preservation are inoculated in dress
Have in the eggplant type bottle of activation medium, 28 DEG C of culture 12h carry out activation culture, complete actication of culture；Ke Liben classes after activation
Bacillus are inoculated in Ke Liben series bacillus level liquid culture mediums according to 1% inoculum concentration, level liquid training
Supporting base includes the component of following mass content：Sucrose 3%, sodium nitrate 0.15%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.05%,
Potassium chloride 0.05%, ferric sulfate 0.002%, balance of water.The temperature of Ke Liben series bacillus level liquid cultures is 30 DEG C,
Initial pH value is 7.5, into culture 24h according to 15m3/ h throughputs carry out ventilation culture, according to 20m after Liquid Culture 24h3/
The throughput of h carries out ventilation culture, after illumination cultivation 30h, stops culture, obtains Ke Liben series bacillus primary seed solutions.
The preparation of Ke Liben series bacillus secondary seed solutions：The Ke Liben series bacillus primary seed solution that will be obtained with
10% inoculum concentration is inoculated in Ke Liben series bacillus secondary liquid culture mediums, and secondary liquid culture medium contains including following quality
The component of amount：Sucrose 1.5%, glucose 1.5%, analysis for soybean powder 1.0%, ammonium sulfate 0.05%, dipotassium hydrogen phosphate 0.05%, chlorination
Calcium 0.1%, balance of water.The temperature of secondary liquid culture is 32 DEG C, and pH value is 7.5, and into culture 24h, throughput is
150m3/ h, after culture 24h, throughput is 250m3/ h, stirs 2 times per hour, and each mixing time is 15min, illumination cultivation
After 48h, stop culture, obtain Ke Liben series bacillus secondary seed solutions.
The preparation of Ke Liben series bacillus：The Ke Liben series bacillus secondary seed solution that will be obtained is with 10% inoculum concentration
It is inoculated in Ke Liben series bacillus three-level fluid nutrient mediums, three-level fluid nutrient medium includes the component of following mass content：
Glucose 2.5%, corn flour 1%, ammonium sulfate 0.05%, dipotassium hydrogen phosphate 0.05%, calcium chloride 0.1%, balance of water.Three-level
The temperature of Liquid Culture is 30 DEG C, and pH value is 7.5, and into culture 24h, throughput is 2000m3/ h, after culture 24h, ventilation
It is 1000m3/h to measure, and is stirred 2 times per hour, and each mixing time is 15min, after illumination cultivation 65h, stops culture, is obtained gram
In this series bacillus third stage culture liquid, 20min is centrifuged under the conditions of 5000rpm using butterfly centrifugal machine, obtain supernatant and
Concentrate, the spray-dried tower of concentrate is dried, and obtains Ke Liben series bacillus.
Embodiment 2
The Ke Liben class bacillus 4kg that Example 1 is obtained, slide with 10kg white granulated sugars, 5.5kg cornstarch, 10kg
Stone flour, 5kg kaolin and 20kg water mix 1h, carry out air-flow crushing, and sealing pressure is 0.12Mpa；Discharging sealing gland pressure
Power is 0.15Mpa；Pulse is 0.6Mpa；Feed pressure is 0.85Mpa；It is 240kg/h to crush dnockout speed；Crushing feeds intake
Measure is 850kg/ batches；It is 20% to mediate water rate control；Prilling temperature is controlled to 55 DEG C, is re-dubbed this class gemma in 200IU/mg grams
Bacillus granule.
Embodiment 3
50~1000 times of the Ke Liben class bacillus granules dilute with water that embodiment 2 is prepared, to banded sclerotial blight
Bacteriostatic activity respectively with the Ministry of Agriculture register propiconazole contrast.
1) Ke Liben series bacillus antimicrobial spectrum
By the dilution of Ke Liben fermenation raw liquids, 50 times carry out Antibacterial Activity to 22 kinds of phytopathogens, and experimental result is as schemed
Shown in 1.Result shows that Ke Liben zymotic fluids show different degrees of bacteriostatic activity to 22 kinds of pathogens of all tests,
Ke Liben zymotic fluids have certain inhibitory action to veterinary antibiotics and tobacco Common Diseases, can be used to prevent and treat multiple kinds of crops
Fungal disease, antimicrobial spectrum is wider.
2) this series bacillus granule difference extension rate fungistatic effect in 200IU/mg grams
To probe into the optimal antibacterial extension rate of Ke Liben series bacillus granule, extension rate gradient observation is set to it
Fungistatic effect, is compared with 0.5ppm propiconazole solution, under equal conditions, suppression of the 0.5ppm propiconazoles solution to rice sheath blight disease
Bacterium rate is as shown in table 1 for the fungistatic effect of 80.06%, Ke Liben series bacillus particle dilution agent different multiples.It can be seen that with
The increase of extension rate, Ke Liben series bacillus granule fungistatic effects are on a declining curve, dilution 200 times when fungistatic effect
Equivalent to the fungistatic effect of 0.5ppm propiconazole solution, under the conditions of 1000 times of dilution, still have to Rhizoctonia solani Kuhn certain
Inhibition.
This series bacillus particle dilution agent different multiples bacteriostasis rate in 1 gram of table
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of Ke Liben series bacillus granule, it is characterised in that the component including following parts by weight：
4 parts of Ke Liben series bacillus；
13~18 parts of auxiliary agent；
55~68 parts of filler；
18~20 parts of water.
2. granule according to claim 1, it is characterised in that the biological guarantor of described Ke Liben class bacillus strains
Tibetan number is CGMCCNo.7996.
3. granule according to claim 1, it is characterised in that the auxiliary agent includes white granulated sugar and cornstarch, described
The mass ratio of white granulated sugar and cornstarch is 1：(4~7.5).
4. granule according to claim 1, it is characterised in that described filler includes talcum powder and kaolin；The cunning
Stone flour and kaolinic mass ratio are (1~2.5)：1.
5. granule according to claim 1, it is characterised in that the content of Ke Liben classes bacillus in the granule
It is 190~210IU/mg.
6. granule according to claim 1, it is characterised in that loss on drying≤6% of the granule.
7. granule according to claim 1, it is characterised in that the pH value of the granule is 6~8.
8. granule according to claim 1, it is characterised in that suspensibility >=80% of the granule.
9. the preparation method of granule described in claim 1, comprises the following steps：By Ke Liben series bacillus, auxiliary agent, filler
Stirred with after water mixing, carry out air-flow crushing, obtain granule.
10. the granule that granule described in claim 1~8 any one or preparation method described in claim 9 are obtained is anti-
Control the application in rice sheath blight disease.
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| CN201710323746.XA CN106929457B (en) | 2017-05-10 | 2017-05-10 | Paenibacillus kribbensis granules and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728380A (en) * | 2018-06-06 | 2018-11-02 | 浙江省农业科学院 | One plant to bacterial strain of the Chinese cabbage hyphal cluster germ with antagonism and its cultural method and application |
| CN109438087A (en) * | 2018-12-27 | 2019-03-08 | 天津环微生物科技有限公司 | A kind of instant granular type agricultural microbial agent and preparation method and application method |
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| CN102870816A (en) * | 2012-10-29 | 2013-01-16 | 黄永 | Preparation of microcapsules of viable microbe biopesticide |
| CN103756931A (en) * | 2013-12-05 | 2014-04-30 | 江西天人生态股份有限公司 | Paenibacillus kribbensis and its application |
| CN106376602A (en) * | 2016-08-31 | 2017-02-08 | 江西天人生态股份有限公司 | Paecilomyces lilacinus granule and preparation method thereof |
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2017
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Patent Citations (3)
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| CN102870816A (en) * | 2012-10-29 | 2013-01-16 | 黄永 | Preparation of microcapsules of viable microbe biopesticide |
| CN103756931A (en) * | 2013-12-05 | 2014-04-30 | 江西天人生态股份有限公司 | Paenibacillus kribbensis and its application |
| CN106376602A (en) * | 2016-08-31 | 2017-02-08 | 江西天人生态股份有限公司 | Paecilomyces lilacinus granule and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728380A (en) * | 2018-06-06 | 2018-11-02 | 浙江省农业科学院 | One plant to bacterial strain of the Chinese cabbage hyphal cluster germ with antagonism and its cultural method and application |
| CN108728380B (en) * | 2018-06-06 | 2020-07-31 | 浙江省农业科学院 | Strain with antagonistic action on Chinese cabbage sclerotinia sclerotiorum and culture method and application thereof |
| CN109438087A (en) * | 2018-12-27 | 2019-03-08 | 天津环微生物科技有限公司 | A kind of instant granular type agricultural microbial agent and preparation method and application method |
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