AU2021104051A4

AU2021104051A4 - Microbial soil conditioner and preparation method thereof

Info

Publication number: AU2021104051A4
Application number: AU2021104051A
Authority: AU
Inventors: Xue JIANG; Xiangyun Li; Jintao Liu; Shutang Liu; Anzhen Wang; Min Wang; Wenliang Wei
Original assignee: Qingdao Agricultural University
Current assignee: Qingdao Agricultural University
Priority date: 2021-07-12
Filing date: 2021-07-12
Publication date: 2021-09-09
Anticipated expiration: 2029-07-12

Abstract

The invention provides a microbial soil conditioner and a preparation method thereof. The raw material composition of the soil conditioner, in parts by weight, includes: 0.4-2 parts of compound microbial inoculum, 1.5-3 parts of phosphatase, and modified medical stone 10-15 parts, 1-4 parts of attapulgite, 3-6 parts of shell mineral powder, 6-12 parts of seaweed powder, 6-12 parts of soybean meal powder, 6-12 parts of coconut shell powder, 0.6-3 parts of accelerator, 5-10 parts of hydroxypropyl-p-cyclodextrin. The preparation process includes the steps of probiotic culture, ingredients, strain insertion, granulation, and drying. The soil conditioner prepared by the above formula and preparation method has good water absorption capacity and strong water retention capacity, and the water absorption rate can reach 200 240g/g. After the accelerated drying test at 80°C, the water retention time can reach 20-25h, and it has excellent effects of improving soil structure and improving soil fertility. It is a soil conditioner suitable for popularization.

Description

Microbial soil conditioner and preparation method thereof

TECHNICAL FIELD

The invention belongs to the technical field of soil improvement, and particularly relates

to a microbial soil conditioner and a preparation method thereof.

BACKGROUND

Improving soil fertility and promoting soil to maintain productivity to crops have

increasingly become the key concern of the country, society and many scientific research

institutions. Whether the problem can be successfully solved is also a key point related to

population formation and social supply.

In the field of crop cultivation nowadays, it is often involved to apply a large amount of

chemical fertilizers, pesticides, herbicides, pesticides and other substances, which is a

common method to increase production. However, the method can cause the content

imbalance of soil nutrients, soil hardening, bacteria increase and soil fertility decline,

thereby affecting the yield and quality of crops and more seriously causing the change of

the soil self-property to affect the sustainable agricultural production. Currently, an

important strategy to address this problem is the application of a variety of soil conditioners

to adjust soil composition and improve soil performance, thereby maintaining soil

moisture, nutrients, soil fertility, and increasing fertilizer bioavailability.

The invention patent CN107474853A provides a biological soil conditioner and its

preparation method. The components include: mixture A 50-60kg; plant ash 10-30kg;

potassium humate 5-20kg; weathered coal 5-20kg; slaked lime 5-20kg, Powder type

silicate bacteria 0.1 1kg; powder type bacillus subtilis 0.3 3kg; soil conditioner

contains humic acid and other organic matter can effectively improve the soil aggregate structure, with quick-acting and long-lasting acid soil treatment effect, but, This kind of soil conditioner has insufficient water retention capacity and is not suitable for dry and water-deficient areas.

The invention patent CN104263375B discloses a composite soil conditioner and a

preparation method thereof, the composite soil conditioner comprises biogas residue, wood

vinegar liquid, mineral powder, soluble chitosan, diethylenetriamine pentaacetate, a

surfactant, cigarette powder, trehalose, double rhamnolipid, a flocculation component, a

penetrating agent, enteromorpha powder, a modified zeolite, a boron-added zinc

magnesium type microbial inoculum, a nano additive, a accelerator, a pentaerythritol

triacrylate and an ionic liquid, The composite conditioner can improve the pH of the soil,

improve the permeability of the soil and reduce the cadmium content in the soil, but the

components of the composite conditioner are quite complex, stratification and precipitation

phenomena are easy to exist, and the stability of the product needs to be improved.

Although people have developed a variety of soil conditioners, the soil improvement effect

of these conditioners is unsatisfactory, and appropriate synergistic components or

accelerators still need more research and development efforts to explore and find.

SUMMARY

In order to solve the problems existing in the prior art and further optimize the prior art,

the invention provides a microbial soil conditioner and a preparation method thereof, so as

to achieve the following invention purposes:

1. Improve the water retention capacity of the microbial soil conditioner;

2. Improve product stability;

3. Improve the soil structure, improve soil fertility;

In order to solve that technical problems, the invention adopt the following technical

scheme:

A microbial soil conditioner is characterized by comprising the following raw materials in

parts by weight: 0.4-2 parts of composite microbial inoculum, 1.5-3 parts of phosphatase,

-15 parts of modified medical stone, 1-4 parts of attapulgite, 3-6 parts of shell mineral

powder, 6-12 parts of seaweed powder, 6-12 parts of soybean meal powder, 6-12 parts of

coconut shell powder, 0.6-3 parts of accelerator, 5-10 parts of hydroxypropyl-p

cyclodextrin;

The composite microbial inoculum comprise bacillus subtilis and bacillus laterosus, and

that composite microbial inoculum contains 100-120 billion strain /g in total;

The mass ratio of accelerator, coconut diethanolamide and disodium lauryl sulfosuccinate

is 3-5:1;

The specific surface area of that modify medical stone is 20-40m2 /g;

The preparation method of that modify medical stone comprises the follow steps of:

cleaning the medical stone by using clear water, and crushing into medical stone particles

with the size of 10-25 m by using special equipment; Heating the medical stone particles

from normal temperature to 300°C at the rate of 30°C/min under an anaerobic condition,

preserving heat for 2h, and then heating to 800°C at the rate of 10°C/min for 5 to 6 h;

Preferably, 4-4.2mol/L phosphoric acid solution can also be added into the medical stone,

stirred for 1 hour at the rate of 150r/min at 90°C, and put into a hot air circulation drying

cylinder at 100°C-120°C for drying for standby.

The preparation method of the microbial soil conditioner is characterized in that the

preparation method comprises the steps of probiotic culture, burdening, strain inoculation,

granulation and drying;

According to that probiotic culture, after culture, the saccharification forces of bacillus

subtilis and bacillus laterosus both reach more than 90mg/g.h;

The mas ratio of that cultured bacillus subtilis to the bacillus laterosus is 1-1.3:1;

Granulating to obtain wet soil conditioner particles with the particle diameter of 2-4mm;

The drying is carried out at the temperature of between 200 and 230°C until the water

content is between 10 and 15%.

By adopting the technical scheme, the invention has the beneficial effects that:

1. By adopting the microbial soil conditioner, the microbial soil conditioner has strong

water retention capacity, the water absorption rate can reach 200-240g/g, and the water

retention time can reach 20-25 h through an accelerated drying test at 80°C;

2. By adopting the preparation method of the microbial soil conditioner, the product of the

conditioner has good stability and does not deteriorate after being placed at normal

temperature for 36 months;

3. The microbial soil conditioner can improve the soil structure and the soil fertility;

Applying the soil conditioner in spring according to the dosage of 35-40kg/ mu, plowing

the soil, wherein the microbial quantity in the soil can reach 2.0-2.3 x10 6/g dry soil, and the

microbial quantity is kept at 95% or above after 30 days;

4. By adopting the microbial soil conditioner provided by the invention, aiming at medium

and mild salinization soil, the salt content of the 0-30cm soil layer can reach 0.13-0.17%, the soil pH value is 6.8-7.3, the soil bulk density is 0.9-0.95 g/cm3 , and the air permeability gap of the soil is 14-18%.

DESCRIPTION OF THE INVENTION

The invention is further explained below in connection with specific embodiments.

Embodiment 1 A microbial soil conditioner and a preparation method thereof

The microbial soil conditioner comprises the following raw materials in parts by weight:

0.4 part of composite microbial inoculum, 1.5 part of phosphatase, 10 parts of modify

medical stone, 1 part of attapulgite, 3 parts of shell mineral powder, 6 parts of seaweed

pow, 6 parts of soybean meal powder, 6 parts of coconut shell powder, 0.6 part of

accelerator and 5 parts of hydroxypropyl-p-cyclodextrin;

The composite microbial inoculum comprise bacillus subtilis and bacillus laterosus and

contains 100 billion strain /g in total;

The shell mineral pow is calcined at 500°C for 6h, and that average particle size is 80 mesh;

The specific surface area of that modify medical stone is 20.2m2 /g;

1) cleaning medical stone with clear water, and pulverizing into 10-15 m medical stone

particles by special equipment;

2) heating the medical stone particles from normal temperature to 300°C at the rate of

°C/min under an anaerobic condition for 2h, and then heating to 800°C at the rate of

°C/min for 5h to obtain the modified medical stone;

The preparation method of the microbial soil conditioner comprises the following steps:

1. Probiotics culture

Inoculate that strains into a potato culture medium respectively under a sterile condition,

and culturing for 3 days at the constant temperature of 28°C in a constant temperature box;

Inoculating to a triangular flask for expanded culture for 48 hours, buckling the flask after

the hyphae are covered with the culture medium, continuously culturing for 24 hours after

buckling, taking out the flask for drying at the drying temperature of 35°C, and packaging

in a sterile and dry paper bag;

bacillus subtilis and bacillus laterosus were cultured separately. After culture, the

saccharification force was 90.0 and 90.6 mg/g h, respectively.

2. Ingredients

Weighing the modified medical stone, the attapulgite, the shell mineral powder, the

seaweed powder, the soybean meal powder and the coconut shell powder according to the

proportion, putting the mixture into a disc stirrer to be mixed evenly, adding an accelerator,

transferring the mixture into a sand grinder and carrying out wet grinding for 120min at the

rotating speed of 1Om/sec to obtain mixed powder;

The accelerator includes coconut diethanolamide and disodium lauryl sulfosuccinate, and

the mass ratio of coconut diethanolamide and disodium lauryl sulfosuccinate is 3:1;

Uniformly mixing phosphatase and hydroxypropyl-p-cyclodextrin according to a

proportion, and adding water to prepare enzyme solution with the concentration of 8wt%;

3. Strain inoculation

Inoculating the cultured bacillus subtilis and bacillus laterosus strains into the mixed

powder according to a proportion, uniformly spraying enzyme liquid, and uniformly

stirring by a stirrer;

The mas ratio of that cultured bacillus subtilis to the bacillus laterosus is 1: 1; The active

bacterial count of the composite microbial bacteria is 3.2x108 /g;

4. Granulation

Transferring the mixed materials into a rotary drum granulator to prepare wet soil

conditioner particles with the particle diameter of 2-3mm;

5. Air drying:

The wet soil conditioner particles are air-dried to the water content of 12 to 16%, and

packaged to obtain the microbial soil conditioner.

The microbial soil conditioner prepared by the technical scheme of embodiment 1 has the

water absorption up to 200g/g, and the water retention time up to 21 h through an

accelerated drying test at 80°C;

The soil conditioner is used for treating the medium and mild salinization soil test field 1,

the soil pH is 4.5 and the salt content is 0.38% before treatment;

When the soil conditioner was applied in spring with the dosage of 40kg/ mu, the number

of microorganisms in the soil could reach 2.0x106 /g dry soil after plowing.

After being used for one month, the salt content of the 0-30cm soil layer can be reached to

be 0.17%, the soil pH value is 6.6, the soil bulk density is 0.90 g/cm3 , and the air

permeability gap of the soil is 14.1%; The numb of microorganisms still reach 95%.

Embodiment 2 A microbial soil conditioner and a preparation method thereof

1 part of composite microbial inoculum, 2 parts of phosphatase, 10 parts of modified

medical stone, 3 parts of attapulgite, 5 parts of shell mineral powder, 6 parts of seaweed powder, 10 parts of soybean meal powder, 10 parts of coconut shell powder, 2 parts of accelerator and 10 parts of hydroxypropyl-p-cyclodextrin;

contains 100 billion strain /g in total;

The specific surface area of that modify medical stone is 32.0m2 /g;

1) cleaning medical stone with clear water, and pulverizing into 20-25 m medical stone

particles by special equipment;

°C/min for 6h to obtain the modified medical stone preform;

3) adding 4.2 mol/L phosphoric acid solution accounting for 20% of the total amount of

the medical stone preform, stirring for 1 hour at the rate of 150 r/min at 90°C, and drying

in a hot air circulation drying cylinder at 120°C to obtain the modified medical stone for

later use;

1. Probiotics culture

the hyphae are covered with the culture medium, continuously culturing for 24 hours after buckling, taking out the flask for drying at the drying temperature of 35°C, and packaging in a sterile and dry paper bag;

Bacillus subtilis and bacillus laterosus were cultured separately, and the saccharification

force reached 90.2 and 91.0 mg/g h respectively after culture.

2. Ingredients

transferring the mixture into a sand grinder and carrying out wet grinding for 120 min at

the rotating speed of 15 m/sec to obtain mixed powder;

The accelerator comprise coconut diethanolamide and disodium lauryl sulfosuccinate,

wherein that mass ratio of the coconut diethanolamide to the disodium lauryl sulfosuccinate

is 5: 1;

Uniformly mixing phosphatase and hydroxypropyl-p-cyclodextrin according to a

3. Strain inoculation

Respectively inoculating the cultured bacillus subtilis and bacillus laterosus strains into

the mixed powder according to a proportion, uniformly spraying the mixed enzyme liquid,

and uniformly stirring by a stirrer;

The mass ratio of that cultured bacillus subtilis to the bacillus laterosusis 1.2: 1; The active

bacteria content of the composite microbial inoculum is 3.4x108 /g;

4. Granulation

conditioner particles with the particle diameter of 2-4mm;

5. Air drying:

packaged to obtain the microbial soil conditioner.

The biological soil conditioner prepared by the technical scheme of embodiment 2 has the

water absorption up to 240 g/g, and the water retention time up to 24 h through an

accelerated drying test at 80°C;

The soil conditioner is used for treating the medium and mild salinization soil test field 2,

the soil pH is 5.3 and the salt content is 0.36% before treatment;

Applying the soil conditioner in spring according to the dosage of 35kg/ mu, plowing the

soil, and the microbial quantity in the soil can reach 2.2x106 /g dry soil;

be 0.13%, the soil pH value is 7.0, the soil bulk density is 0.93 g/cm3 , and the air

permeability gap of the soil is 15.2%; The numb of microorganisms remain above 98%.

Embodiment 3 A microbial soil conditioner and a preparation method thereof

2 parts of composite microbial inoculum, 3 parts of phosphatase, 15 parts of modified

medical stone, 4 parts of attapulgite, 6 parts of shell mineral powder, 12 parts of seaweed

powder, 6 parts of soybean meal powder, 12 parts of coconut shell powder, 3 parts of

accelerator and 5 parts of hydroxypropyl-p-cyclodextrin;

contains 120 billion strain /g in total;

The shell mineral pow is calcined at 500°C for 6h, and that average particle size is 100

mesh;

The specific surface area of that modify medical stone is 38.8m2 /g;

1) cleaning medical stone with clear water, and pulverizing into medical stone particles of

-25 m by special equipment;

°C/min for 5h to obtain the medical stone preform;

3) adding 4.2mol/L phosphoric acid solution accounting for 20% of the total amount of the

medical stone preform, stirring for 1 hour at the rate of 150r/min at 90°C, and drying in a

hot air circulation drying cylinder at 120°C to obtain the modified medical stone for later

use;

1. Probiotics culture

Inoculate that strains into a potato culture medium respectively unde a sterile condition,

and culturing for 3 days under the constant temperature of 32°C in a constant temperature

box; Then inoculating to a triangular bottle for expanded culture for 72 hours, buckling the

bottle after hyphae are covered with the culture medium, continuing culture for 24 hours

after buckling the bottle, taking the bottle out for drying at the drying temperature of 40°C,

and packaging in a sterile and dry paper bag;

force reached 90.2 and 90.8 mg/g h respectively after culture.

2. Ingredients

the rotating speed of 15 m/sec to obtain mixed powder;

wherein that mas ratio of the coconut diethanolamide to the disodium lauryl sulfosuccinate

is 5: 1;

Uniformly mixing phosphatase and hydroxypropyl-p-cyclodextrin according to a

proportion, and adding water to prepare enzyme solution with the concentration of1Owt%;

3. Strain inoculation

and uniformly stirring by a stirrer;

The mas ratio of that cultured bacillus subtilis to the bacillus laterosus is 1.3: 1; The active

bacteria content of the composite microbial inoculum is 3.8x108 /g;

4. Granulation

conditioner particles with the particle diameter of 2-4mm;

5. Air drying:

Dry that wet soil conditioner particle until the water content is 15%, and packaging to

obtain the microbial soil conditioner.

The biological soil conditioner prepared by the technical scheme of embodiment 3 has the

water absorption up to 220 g/g, and the water retention time up to 20 h through an

accelerated drying test at 80°C;

The soil conditioner is used for treating the medium and mild salinization soil test field 3,

the soil pH is 5.0 and the salt content is 0.36% before treatment;

soil, and the microbial quantity in the soil can reach 2.3 x106 /g dry soil;

be 0.16%, the soil pH value is 6.9, the soil bulk density is 0.95 g/cm 3, and the air

permeability gap of the soil is 17.7%; The numb of microorganisms remain at 98% and

above.

Comparative embodiment 1:

The invention provides a microbial soil conditioner, which comprises the following

components in parts by weight:

1 part of composite microbial inoculum, 2 parts of phosphatase, 10 parts of common

medical stone, 4 parts of attapulgite, 5 parts of shell mineral powder, 10 parts of seaweed

powder, 10 parts of soybean meal powder, 6 parts of coconut shell powder, 3 parts of

accelerator and 10 parts of hydroxypropyl-p-cyclodextrin;

The common medical stone is purchased on the market and a 600-mesh product is directly

used;

The microbial soil conditioner prepared from the raw materials through the processes of

probiotic culture, ingredient preparation, strain inoculation, granulation and drying has a water absorption rate of 166g/g and is subjected to an accelerated drying test at 80°C for a water retention time of 15 h;

The soil conditioner is used for treating the medium and mild salinization soil test field 4,

the soil pH is 5.4 and the salt content is 0.36% before treatment;

Applying the soil conditioner in spring according to the dosage of 35kg/ mu, and plowing

the soil, wherein the microbial quantity in the soil is 2.01x106 /g dry soil;

After one month of use, it is possible to achieve a soil layer of 0-30cm with a salt content

of 0.16%, a soil pH of 7.2, a soil bulk density of 0.82g/cm3 , and a soil porosity of 15.1%.

Finally, it should be noted that the above description is only of the preferred embodiments

of the present invention and is not intended to limit the invention. Although the present

invention has been described in detail with reference to the foregoing embodiments, it is

still possible for those skilled in the art to modify the technical solutions described in the

foregoing embodiments, or equivalently substitute some of the technical features thereof.

Any modifications, equivalents, improvements, etc,that come within the spirit and

principles of the invention are intended to be included within the scope of the invention.

Claims

THE CLAIMS DEFINING THE INVENTION ARE AS FOLLOWS:

1. A soil conditioner, characterized by comprising the following raw materials in parts by

weight: 0.4-2 parts of microbial mixture, 1.5-3 parts of phosphatase, 10-15 parts of

modified medical stone, 1-4 parts of attapulgite, 3-6 parts of shell mineral powder, 6-12

parts of seaweed powder, 6-12 parts of soybean meal powder, 6-12 parts of coconut shell

powder, 0.6-3 parts of accelerator and 5-10 parts of hydroxypropyl-p-cyclodextrin.

2. The soil conditioner according to claim 1, wherein the microbial mixture comprises

bacillus subtilis and bacillus laterosus, and the microbial mixture contains 100-120 billion

bacteria per g in total.

3. the soil conditioner according to claim 1, wherein the mass ratio of the accelerator, the

coconut diethanolamide and the disodium lauryl sulfosuccinate is 3-5:1.

4. The soil conditioner according to claim 1, wherein the specific surface area of the

modified medical stone is 20-40 m2 /g.

5. The soil conditioner according to claim 4, wherein the preparation method of the

modified medical stone comprises the following steps of: firstly cleaning the medical stone

with clear water, and crushing into medical stone particles with the size of 10-25 m by

special equipment; Then heating the medical stone particles from normal temperature to

300°C at the rate of 30°C/min under an anaerobic condition, preserving the heat for 2h, and

then heating to 800°C at the rate of 10°C/min for 5-6 h.

6. The soil conditioner according to claim 5, wherein the preparation method of the

modified medical stone further comprises adding 4-4.2mol/L phosphoric acid solution into

the medical stone, stirring at a rate of 150 r/min for 1 hour at 90°C, and drying in a hot air

circulation drying cylinder at 100-120°C for standby.

7. A preparation method of a soil conditioner, characterized in that the preparation method

comprises the observation of a probiotics mixture, strain observation and drying.

8. The preparation method according to claim 7, wherein after the probiotics bacteria are

observed, the saccharification forces of bacillus subtilis and bacillus laterosusboth reach

more than 90mg/g.h.

9. The preparation method according to claim 7, wherein the mass ratio of the observed

bacillus subtilis to the cultured bacillus laterosus is 1-1.3:1

.

10. The preparation method according to claim 7, characterized in that the drying is carried

out at a temperature of 200-230°C to a moisture content of 10-15%.