CN106927570B - Low-short flower-free tape grass-based plant-bacterium symbiotic underwater lawn - Google Patents
Low-short flower-free tape grass-based plant-bacterium symbiotic underwater lawn Download PDFInfo
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- CN106927570B CN106927570B CN201610667017.1A CN201610667017A CN106927570B CN 106927570 B CN106927570 B CN 106927570B CN 201610667017 A CN201610667017 A CN 201610667017A CN 106927570 B CN106927570 B CN 106927570B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Biodiversity & Conservation Biology (AREA)
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Abstract
The invention discloses a low-dwarf flower-free tape grass-based symbiotic underwater lawn for planting bacteria. It is formed by culturing according to the following method: sterilizing the pond bottom material by using quick lime; planting short flower-free tape grass; splashing bleaching powder into the pond; after 6-8 weeks, forming a low and flower-free underwater lawn in the pond; adjusting the pH value in the pond to 8-9 by using calcium peroxide; after three days, selecting the sunny day for inoculation of the immobilized nitrobacteria; sprinkling the immobilized nitrobacteria particles or nitrobacteria powder in the whole pool, and quickly growing and propagating the immobilized nitrobacteria on the surface of the low flower-free tape grass; after one month, a biofilm with nitrifying bacteria as the main component is formed on the surface of the low flower-free tape grass, and a plant-bacterium symbiont with the low flower-free tape grass as a plant and immobilized nitrifying bacteria as bacteria bodies is formed. The low flower-free tape grass-based plant-fungus symbiotic underwater lawn greatly enhances the actual purification capacity of submerged plants in water.
Description
Technical Field
The invention belongs to the technical field of ecological restoration of lakes, rivers and landscape water bodies, and relates to a low flower-free tape grass-based plant-fungus symbiotic underwater lawn.
Background
The damage of the ecological environment of the water area in China and the loss of the self-purification capability of the water body are common phenomena caused by the pollution of the water body. The method for treating water pollution by using water body ecological restoration is a common method, and the short flower-free tape grass as a submerged plant is widely applied to water ecological restoration all over the country. The submerged plant has a purification effect on water quality, except that nitrogen and phosphorus in water are consumed for growth of the submerged plant, a biological membrane formed by various bacteria on the surface of the plant has a larger purification effect on the water quality, and particularly nitrifying bacteria in the biological membrane are the most important elements for reducing ammonia nitrogen in the water. The elimination of ammonia nitrogen is the key point for solving the problems of black and odorous water body and blue algae outbreak. Since nitrifying bacteria are autotrophic bacteria, are very sensitive to changes of environmental factors and grow very slowly relative to other bacteria, the ecological niche on the surface of a submerged plant is very easily occupied by other various bacteria randomly without directional guidance in a natural state or a general cultured submerged plant, and the nitrifying bacteria are only a few of the nitrifying bacteria. Furthermore, the general submerged plants are seasonal, and when the submerged plants disappear, the biofilm layer attached to the submerged plants does not exist.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a low-bush tape grass-based plant bacteria symbiotic underwater lawn which can enable a biological film on the surface of a plant to exist in a water body all the time, and the bacteria in the biological film are mainly nitrifying bacteria.
The purpose of the invention is realized by the following technical scheme:
a kind of vegetation fungus symbiotic underwater lawn based on short and without flower bitter grass, it is cultured and formed according to the following method:
(1) 180 and 220kg of quicklime (calcium oxide) per mu are put into the pond to disinfect the bottom materials of the pond; one week later, planting the low flower-free tape grass in the pond with the depth of 25-35cm, taking a bundle of 20-30cm as the interval in longitudinal and transverse directions and 3-4 plants in each bundle, adding the pond water to 45-55cm to cultivate the underwater lawn of the low flower-free tape grass;
(2) splashing bleaching powder into the pond 2-3 times per week, wherein the concentration of the bleaching powder in the water of the pond is 5-10ppm for killing and inhibiting the growth of other microorganisms; after 6-8 weeks, forming a low and flower-free underwater lawn in the pond; after one week of the last bleaching powder splashing, adjusting the pH value in the pond to be 8-9 by using calcium peroxide;
(3) after three days, selecting the daytime (preferably 9-10 am) of sunny days to inoculate the immobilized nitrobacteria; the content is 1 x 109-1*1011The immobilized nitrobacteria particles or nitrobacteria powder per gram are 1.2-1.8g/m2The concentration of the water is splashed in the whole pool once every two days for three to four times; because the nitrobacteria powder is immobilized, the nitrobacteria powder is automatically activated after meeting water, and the fixed point is searched by flagellum movement, the surface of the low flower-free tape grass is rich in oxygen in daytime, and CO breathed out by grass is rich2Is the best carbon source of nitrifying bacteria, therefore, the surface of the low flower-free tape grass is the best solid of the immobilized nitrifying bacteria, and the immobilized nitrifying bacteria grow and reproduce on the surface quickly;
(4) after one month, a biofilm with nitrifying bacteria as the main component is formed on the surface of the low flower-free tape grass, so that a plant-bacterium symbiont with the low flower-free tape grass as a plant and immobilized nitrifying bacteria as bacteria bodies is formed.
The invention has the beneficial effects that:
the invention selects evergreen short flower-free tape grass as submerged plant, and makes the water body always have the existence of biological film on the surface of the plant, and makes the biological film on the surface of the short flower-free tape grass be the biological film which is mainly formed by nitrobacteria through the processes of disinfection, sterilization and nitrobacteria inoculation, so that the actual purification capacity of the submerged plant in the water can be greatly enhanced.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
The invention relates to a low-dwarf-flower-free tape grass-based plant-fungus symbiotic underwater lawn, which comprises the following culture processes:
(1) 200kg of quicklime (calcium oxide) per mu is put into the pond to disinfect the bottom materials of the pond; planting low-bush tape grass in the pond after one week under the condition that the depth of the pond water is 30cm, taking a cluster of 25cm as a space in the longitudinal and transverse directions, and adding pond water to 50cm to cultivate the underwater lawn of the low-bush tape grass, wherein 3-4 plants are planted in each cluster;
(2) splashing bleaching powder into the pond twice a week, wherein the concentration of the bleaching powder in the water of the pond is 5-10ppm for killing and inhibiting the growth of other microorganisms; forming a low and short underwater lawn without flowers and bitter grasses in the pond after 6 to 8 weeks; after one week of the last bleaching powder splashing, adjusting the pH value in the pond to be 8-9 by using calcium peroxide;
(3) after three days, selecting about 9 am on a sunny day to inoculate the immobilized nitrobacteria; the content is 1 x 1010Per g of immobilized nitrifying bacteria particles at 1.5g/m2The concentration of the fertilizer is sprayed in the whole pool, once every other day and three times continuously; because the nitrobacteria powder is immobilized, the nitrobacteria powder is automatically activated after meeting water, and the fixed point is searched by flagellum movement, the surface of the low flower-free tape grass is rich in oxygen in daytime, and CO breathed out by grass is rich2Is the best carbon source of nitrifying bacteria, therefore, the surface of the low flower-free tape grass is the best solid of the immobilized nitrifying bacteria, and the immobilized nitrifying bacteria grow and reproduce on the surface quickly;
(4) after one month, a biofilm with nitrifying bacteria as the main component is formed on the surface of the low flower-free tape grass, so that a plant-bacterium symbiont with the low flower-free tape grass as a plant and immobilized nitrifying bacteria as bacteria bodies is formed.
The low flower-free tape grass-based plant bacteria symbiotic underwater lawn enables a biological film on the surface of a plant to exist in a water body all the time, bacteria in the biological film are mainly nitrifying bacteria, and the plant bacteria symbiont greatly enhances the actual purification capacity of submerged plants in water.
Example 2
The invention relates to a low-dwarf-flower-free tape grass-based plant-fungus symbiotic underwater lawn, which comprises the following culture processes:
(1) putting 180kg of quicklime per mu into the pond to disinfect the pond substrate; one week later, planting the low flower-free tape grass in the pond under the condition that the depth of the pond water is 25cm, taking one bundle of 20cm in length and breadth as the interval, adding the pond water to 45cm to cultivate the underwater lawn of the low flower-free tape grass;
(2) splashing bleaching powder into the pond 2 times a week, wherein the concentration of the bleaching powder in the water of the pond is 10 ppm; after 6-8 weeks, forming a low and flower-free underwater lawn in the pond; after one week of the last bleaching powder splashing, adjusting the pH value in the pond to be 8-9 by using calcium peroxide;
(3) after three days, selecting 10 am on a sunny day to inoculate the immobilized nitrobacteria; the content is 1 x 1011The bacterial powder of nitrobacteria is 1.2g/m2The concentration of the water is splashed in the whole pool, once every other day and four times continuously; the nitrobacteria fixing powder is automatically activated after meeting water, and a fixing point is searched by flagellum movement, so that the nitrobacteria can quickly grow and propagate on the surface of the low flower-free tape grass;
(4) after one month, a biofilm with nitrifying bacteria as the main component is formed on the surface of the low flower-free tape grass, so that a plant-bacterium symbiont with the low flower-free tape grass as a plant and immobilized nitrifying bacteria as bacteria bodies is formed.
The low flower-free tape grass-based plant bacteria symbiotic underwater lawn enables a biological film on the surface of a plant to exist in a water body all the time, bacteria in the biological film are mainly nitrifying bacteria, and the plant bacteria symbiont greatly enhances the actual purification capacity of submerged plants in water.
Example 3
The invention relates to a low-dwarf-flower-free tape grass-based plant-fungus symbiotic underwater lawn, which comprises the following culture processes:
(1) 220kg of quicklime per mu is put into the pond to disinfect the pond bottom materials; one week later, planting the low flower-free tape grass in the pond under the condition that the depth of the pond water is 35cm, taking one cluster of 30cm in length and breadth as the interval, 3 plants in each cluster, adding the pond water to 55cm to cultivate the underwater lawn of the low flower-free tape grass;
(2) splashing bleaching powder into the pond for 3 times every week, wherein the concentration of the bleaching powder in the water of the pond is 5 ppm; after 6-8 weeks, forming a low and flower-free underwater lawn in the pond; after one week of the last bleaching powder splashing, adjusting the pH value in the pond to be 8-9 by using calcium peroxide;
(3) after three days, selecting about 9 am and half am on a sunny day to inoculate the immobilized nitrobacteria; the content is 1 x 109Per g of immobilized nitrifying bacteria particles at 1.8g/m2The concentration of the water is splashed in the whole pool once every two days and three times continuously; the nitrobacteria fixing powder is automatically activated after meeting water, and a fixing point is searched by flagellum movement, so that the nitrobacteria can quickly grow and propagate on the surface of the low flower-free tape grass;
(4) after one month, a biofilm with nitrifying bacteria as the main component is formed on the surface of the low flower-free tape grass, so that a plant-bacterium symbiont with the low flower-free tape grass as a plant and immobilized nitrifying bacteria as bacteria bodies is formed.
The low flower-free tape grass-based plant bacteria symbiotic underwater lawn enables a biological film on the surface of a plant to exist in a water body all the time, bacteria in the biological film are mainly nitrifying bacteria, and the plant bacteria symbiont greatly enhances the actual purification capacity of submerged plants in water.
Claims (7)
1. A kind of vegetation fungus symbiotic underwater lawn based on short and without flower bitter grass, characterized by that, it is cultured and formed according to the following method:
(1) putting 180 and 220kg of quicklime per mu into the pond to disinfect the pond bottom materials; one week later, planting the low flower-free tape grass in the pond with the depth of 25-35cm, taking a bundle of 20-30cm as the interval in longitudinal and transverse directions and 3-4 plants in each bundle, adding the pond water to 45-55cm to cultivate the underwater lawn of the low flower-free tape grass;
(2) splashing bleaching powder into the pond 2-3 times per week, wherein the concentration of the bleaching powder in the water of the pond is 5-10 ppm; after 6-8 weeks, forming a low and flower-free underwater lawn in the pond; after one week of the last bleaching powder splashing, adjusting the pH value in the pond to be 8-9 by using calcium peroxide;
(3) after three days, selecting the sunny day for directional inoculation of the immobilized nitrobacteria; the content is 1 x 109-1*1011The immobilized nitrobacteria particles or nitrobacteria powder per gram are 1.2-1.8g/m2The concentration of the water is splashed in the whole pool once every two days for three to four times; through directional inoculation, directional culture and directional guide, the immobilized nitrobacteria can quickly grow and propagate on the surface of the short flower-free tape grass;
(4) after one month, a biofilm taking nitrifying bacteria as the leading part is formed on the surface of the low flower-free tape grass, so that a plant-bacterium symbiont taking the low flower-free tape grass as a plant and immobilized nitrifying bacteria as bacteria bodies is formed; and the surface of the plants in the water body always has a biological film mainly comprising nitrifying bacteria.
2. The symbiotic underwater lawn of plants based on tape grass of claim 1,
in the step (1), 200kg of quicklime per mu is thrown into the pond to disinfect the bottom materials of the pond; planting the low flower-free tape grass in the pond after one week under the condition that the depth of the pond water is 30cm, taking a cluster of 25cm in length and breadth as an interval, and adding pond water to 50cm to cultivate the underwater lawn of the low flower-free tape grass, wherein 3-4 plants are planted in each cluster.
3. The symbiotic underwater lawn of plants based on tape grass of claim 1,
splashing bleaching powder into the pond twice a week in the step (2), wherein the concentration of the bleaching powder in the water of the pond is 5-10 ppm; forming a low and short underwater lawn without flowers and bitter grasses in the pond after 6 to 8 weeks; after one week of the last bleaching powder sprinkling, the pH value in the pond is adjusted to be between 8 and 9 by using calcium peroxide.
4. The symbiotic underwater lawn of plants based on tape grass of claim 1,
in the step (3), after three days, selecting 9-10 am on a sunny day to inoculate the immobilized nitrobacteria; the content is 1 x 109-1*1011The immobilized nitrobacteria particles or nitrobacteria powder per gram are 1.2-1.8g/m2The concentration of the water is sprinkled in the whole pool once every two days for three to four times continuously.
5. The symbiotic underwater lawn of plants of low bush-free tape grass according to claim 4,
in the step (3), after three days, 9 am in sunny days is selected for inoculation of the immobilized nitrobacteria; the content is 1 x 1010Per g of immobilized nitrifying bacteria particles at 1.5g/m2The concentration of the fertilizer is sprayed in the whole pool, once every other day and three times continuously.
6. The symbiotic underwater lawn of plants of low bush-free tape grass according to claim 4,
in the step (3), after three days, selecting 10 am on a sunny day to inoculate the immobilized nitrobacteria; the content is 1 x 1011The bacterial powder of nitrobacteria is 1.2g/m2The concentration of the water is sprinkled in the whole pool, once every other day and four times continuously.
7. The symbiotic underwater lawn of plants of low bush-free tape grass according to claim 4,
in the step (3), after three days, selecting 9 am and half am on a sunny day to inoculate the immobilized nitrobacteria; the content is 1 x 109Per g of immobilized nitrifying bacteria particles at 1.8g/m2The concentration of the water is sprinkled in the whole pool once every two days for three times continuouslyNext, the process is carried out.
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| CN113526680A (en) * | 2021-08-20 | 2021-10-22 | 上海禹尚水环境科技有限公司 | Water ecosystem construction method guided by symbiosis of fungi and grasses |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101898835A (en) * | 2010-07-26 | 2010-12-01 | 宁波大学 | Method for purifying aquatic water by using microbes and plants in combination |
| CN203922856U (en) * | 2014-05-28 | 2014-11-05 | 上海粤田生物科技有限公司 | Simple underwater lawn |
| CN104803570A (en) * | 2015-05-08 | 2015-07-29 | 广东碧水源生态科技有限公司 | Ecological restoration and purification method for landscape water environment |
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| CN104150604B (en) * | 2014-08-30 | 2016-08-24 | 湖南青水生态环境工程有限公司 | A kind of method utilizing Herba vallisneriae Spiralis to be main submerged plant and photosynthetic bacteria joint mapping subaqual landscape ecosystem |
| CN204490613U (en) * | 2015-03-10 | 2015-07-22 | 上海水源地建设发展有限公司 | Based on the short underwater lawn modular system without flower eel grass |
| CN105417905B (en) * | 2015-12-08 | 2017-11-17 | 上海水源地建设发展有限公司 | A kind of lake water course in situ process for treating sludge based on underwater lawn modular system |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101898835A (en) * | 2010-07-26 | 2010-12-01 | 宁波大学 | Method for purifying aquatic water by using microbes and plants in combination |
| CN203922856U (en) * | 2014-05-28 | 2014-11-05 | 上海粤田生物科技有限公司 | Simple underwater lawn |
| CN104803570A (en) * | 2015-05-08 | 2015-07-29 | 广东碧水源生态科技有限公司 | Ecological restoration and purification method for landscape water environment |
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