CN104150604B - A kind of method utilizing Herba vallisneriae Spiralis to be main submerged plant and photosynthetic bacteria joint mapping subaqual landscape ecosystem - Google Patents

A kind of method utilizing Herba vallisneriae Spiralis to be main submerged plant and photosynthetic bacteria joint mapping subaqual landscape ecosystem Download PDF

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CN104150604B
CN104150604B CN201410436933.5A CN201410436933A CN104150604B CN 104150604 B CN104150604 B CN 104150604B CN 201410436933 A CN201410436933 A CN 201410436933A CN 104150604 B CN104150604 B CN 104150604B
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water
photosynthetic bacteria
submerged plant
water quality
vallisneriae spiralis
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CN201410436933.5A
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Chinese (zh)
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CN104150604A (en
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杨璞
贺岳峰
彭春良
廖菊阳
钟珊
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湖南青水生态环境工程有限公司
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Abstract

A kind of method utilizing Herba vallisneriae Spiralis to be main submerged plant and photosynthetic bacteria joint mapping subaqual landscape ecosystem disclosed by the invention, relates to purification of water quality and garden landscape field, and its key step includes: A, water quality detection;B, removing weeds;C, input photosynthetic bacteria;D, implantation submerged plant.There is water body purification effective, the features such as the later stage is stable, energy consumption is low, simple, the eutrophication of landscape water body can be eliminated fast and effectively, suppression blue algae growth, water transparency is made to reach more than 2m, water quality reaches III class water quality standard, will not produce secondary pollution, again can Green abundant subaqual landscape ecosystem attractive in appearance.

Description

A kind of submerged plant utilizing Herba vallisneriae Spiralis to be master is raw with photosynthetic bacteria joint mapping subaqual landscape The method of state system
Technical field
The present invention relates to purification of water quality and garden landscape field, a kind of submerged plant utilizing Herba vallisneriae Spiralis to be master and light The method closing bacterium joint mapping subaqual landscape ecosystem.
Background technology
Submerged plant is as the primary producer in aquatic ecosystem, and root system affects deposit, and blade then directly affects Water layer, it is possible to play multiple ecological functions.Nutritive salt in the most absorbable water body of submerged plant and bed mud, stores or assimilates For Self substances, the nutritive salt reduced in deposit discharges again;On the other hand can be by carrying out resource contention, secretionization with algae Sense material suppression algal grown breeding.Therefore submerged plant can well purifying water body.
Herba vallisneriae Spiralis (Vallisneria natans (Lour.) Hara) belongs to Vallisneria plant (Vallisneria L.), water The evergreen submerged plant of Trionychidae draft, it has, and toleration is strong, light compensation point is low, fertility is strong, growth is rapid, well developed root system etc. Advantage;Its root stock can be survived the winter and start to sprout when water temperature more than 10 DEG C in spring in next year, therefore, it can after plantation in a year for many years Gather continuously, save the tedious work that other floating bed cultivation plant need again to plant every year.And hydrilla verticillata, potarnogeton lucens Scope is wide, adaptable, fast growth, accumulation ability strong with Ottelia acuminata (Gagnep.) Dandy flower, and suitably collocation uses aquatic ecosystem Stablizing of 26S Proteasome Structure and Function has important function.(number of patent application is Chinese patent: CN03144202) " one is administered with Herba vallisneriae Spiralis The method of lake eutrophication ", it comprises the steps of: 1 cultivates Herba vallisneriae Spiralis;Cultured Herba vallisneriae Spiralis is added lakebed by 2, adapts to 3- 5 days;3. add Herba vallisneriae Spiralis protective agent and algal grown inhibitor.The advantage of the method is that method is simple, and process specifications is low, but Being that floristics is single, effect is limited, and the required cycle is long.
Photosynthetic bacteria has the photosynthesis of uniqueness, has obvious action in terms of reducing water body organism content.It Organic substance in water, ammoniacal nitrogen can be directly utilized, it is also possible to utilize hydrogen sulfide, and the nitrous in water can be removed by Denitrification The pollutant such as acid ammonia.(number of patent application is Chinese patent: 201310639372.4) " a kind of application compound microorganisms is carried out The method of polluted-water restoration of the ecosystem ", described complex microorganism thalline concentrated solution is by the following 10 kinds of thalline groups according to weight portion Become: Serratia: 5-15 part, photosynthetic bacteria: 5-15 part, yeast: 5-15 part, denitrifying bacteria: 5-15 part, lactic acid bacteria: 5-15 part, actinomycetes: 5-15 part, BD antibacterial: 5-15 part, nitrogen-fixing bacteria: 5-15 part, nitrobacteria: 5-15 part, bacillus subtilis Bacterium: 5-15 part, total number of bacteria >=109pic/ml in complex microorganism thalline concentrated solution.Water transparency after reparation 1.5 meters with On, the clearance of algae, total nitrogen, total phosphorus and COD is respectively 95-99%, 95-99%, 90-95%, 90-95%.The method Effect is quickly obvious, but can not realize aesthetic in landscape water body uses.
Photosynthetic bacteria is used in combination with submerged plant, can realize mutually promoting and strengthen absorbed nitrogen phosphorus and organic in water body The effects such as material, suppression algal grown, can more rapid effective purifying water body.Additionally, submerged plant Herba vallisneriae Spiralis, hydrilla verticillata, light leaf Herba potamogetonis distincti uses with the collocation of Ottelia acuminata (Gagnep.) Dandy flower, can greatly enrich subaqual landscape space, vegetation based on Herba vallisneriae Spiralis, hydrilla verticillata and light Leaf Herba potamogetonis distincti then adds in the growth gap of Herba vallisneriae Spiralis, and Ottelia acuminata (Gagnep.) Dandy flower can play the effect of interspersing especially so that smooth hydrospace The change of bulk, cloud form occur, level is distinct, and in picturesque disorder, landscape effect becomes apparent from.Growth along with submerged plant, moreover it is possible to shape Become the seabed Corallium Japonicum Kishinouye effect under transparent water quality, make people pleasing, experience the fine of water scenery.
Summary of the invention
It is an object of the invention to provide a kind of water body purification effective, the later stage is stable, energy consumption is low, simple, and The method that can build good subaqual landscape ecosystem.
For achieving the above object, the solution of the present invention is that a kind of submerged plant utilizing Herba vallisneriae Spiralis to be master of invention is with photosynthetic The method of bacterium joint mapping subaqual landscape ecosystem, its method step is as follows:
A, water quality detection: first the indices of water quality is detected.
B, removing weeds: process waters is carried out in advance the preparations such as weeds removing;
C, input photosynthetic bacteria: being thrown in by photosynthetic bacteria into water body, input ratio is photosynthetic bacterium solution: water body=1:250-350, its In in photosynthetic bacterium solution total number of bacteria be: 3-5 × 1010Cfu/ml, suitably adjusts input ratio according to water quality detection result; Purpose, for reducing water body the content of nitrogen and phosphorous, improves water transparency, provides condition for ensuing submerged plant planting, adds simultaneously Fast submerged plant water purification speed;
D, implant submerged plant: after photosynthetic bacteria repairs water body 20-25 days, by black to four kinds of submerged plant Herba vallisneriae Spiralis, wheel blade Algae, potarnogeton lucens and Ottelia acuminata (Gagnep.) Dandy flower are transplanted in Sediments, and transplanting quantity is based on Herba vallisneriae Spiralis, hydrilla verticillata, light leaf eye Dish and Ottelia acuminata (Gagnep.) Dandy flower use as collocation, and specially Herba vallisneriae Spiralis accounts for the 60%-70% of sum, hydrilla verticillata 10%-15%, light leaf eye Dish 10%-15%, Ottelia acuminata (Gagnep.) Dandy flower 5%-10%, method for transplanting is cuttage or heavy hydroponics or carrier method for transplanting.
Photosynthetic bacteria described in step C has the photosynthesis of uniqueness, and it can directly utilize Organic substance in water, ammonia state Nitrogen, it is also possible to utilize hydrogen sulfide, and can be by pollutant such as the nitrous acid ammonia in Denitrification removal water, photosynthetic bacteria also has There are denitrification activity and the characteristic of long-pending phosphorus, therefore can also obtain preferable denitrogenation and phosphor-removing effect.Meanwhile, photosynthetic bacteria can also Effectively suppress the growth of the harmful algaes such as cyanophyceae, such that it is able to control the generation of " wawter bloom ", advantageously form limpid water attractive in appearance Scape effect.
Described in step D four kind submerged plant is based on Herba vallisneriae Spiralis, hydrilla verticillata, potarnogeton lucens and Ottelia acuminata (Gagnep.) Dandy flower collocation Use.Submerged plant can effectively remove the total nitrogen in sewage, total phosphorus, ammonia due to himself special morphosis and growth characteristics Nitrogen and COD, be obviously improved the content of dissolved oxygen and the transparency of water body simultaneously.Herba vallisneriae Spiralis is to the removal effect of ammonia nitrogen For substantially, can reach 92.01%, next to that the removal to total phosphorus, can reach 90.35%, the removal of total nitrogen is imitated by hydrilla verticillata Fruit is the most obvious, can reach 93.02%, and potarnogeton lucens is the most obvious to the removal effect of total phosphorus, can reach 93.79%, sea Cauliflower also has the strongest assimilation effect to water body nitrogen phosphorus and Organic substance.Four kinds of submerged plants are combined, can more rapid have Effect purifying eutrophication water body, can beneficially keep again ecosystem diversity under water.
The method according to the invention, combines submerged plants such as Herba vallisneriae Spiralis with photosynthetic bacteria, can eliminate fast and effectively The eutrophication of landscape water body, suppresses blue algae growth, makes water transparency reach more than 2m, will not produce secondary pollution, Again can Green abundant subaqual landscape ecosystem attractive in appearance.
According to survey, after repairing water body half a year by the method for the present invention, submerged plant coverage rate reaches more than 80%;Water body Cyanophyceae reduction rate reaches more than 88%;To water body total nitrogen, total phosphorus, COD (COD) and the removal of BOD (Biochemical oxygen demand) Rate all reaches 85-95%;Water transparency improves to more than 2m;Water quality reaches Group III water quality standard.The method is repaiied than ever Faster, effect becomes apparent from the method speed of rehydration ecosystem, and the later stage is more stable, and can form so clear that you can see the bottom, levels are rich, mistake Fall to having cause, make the pleasing subaqual landscape effect of people.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated.Description below is to use the mode enumerated, but this The protection domain of invention should not be limited to this.
Embodiment one:
Repairing place is Changsha about 3000 square meter view waters, and before repairing, water transparency is 0.08m, and water quality is IV Class water quality standard.Concretely comprise the following steps:
A, water quality detection: first the indices of water quality is detected;
B, removing weeds: process waters is carried out the preparations such as weeds removing;
C, throw in photosynthetic bacteria: photosynthetic bacteria is thrown in into water body, input ratio be photosynthetic bacterium solution with water body than 1:350, wherein In photosynthetic bacterium solution, total number of bacteria is: 3-5 × 1010cfu/ml;
D, implant submerged plant: after photosynthetic bacteria repairs water body 20 days, by four kinds of submerged plant Herba vallisneriae Spiralis, hydrilla verticillata, Potarnogeton lucens and Ottelia acuminata (Gagnep.) Dandy flower be transplanted in Sediments, specially Herba vallisneriae Spiralis account for sum 68%, hydrilla verticillata 12%, light leaf eye Sub-dish 14%, Ottelia acuminata (Gagnep.) Dandy spend 6%, and method for transplanting is cuttage.
Throwing in the water body parameter before photosynthetic bacteria is transparency 0.08m, COD 67.4mg/L, BOD 13.9mg/L, TN 11.6mg/L, TP 0.8mg/L, after photosynthetic bacteria processes 20 days, water transparency is adjusted to 0.3-0.8m, COD, BOD, TN 45.2mg/L, 9.6mg/L, 7.6mg/L and 0.4mg/L is reduced to respectively with TP value.
After repairing water body half a year by the method for the present invention, submerged plant coverage rate reaches 80%;The cyanophyceae of water body is cut down Rate reaches 90%;Water body COD, BOD, TN and TP value reduces to 11.2mg/L, 3.0mg/L, 0.8mg/L and 0.04mg/L respectively, goes Except rate reaches 85-95%;Water transparency improves to more than 2m;Water quality reaches Group III water quality standard;Define and limpid seen The end, levels are rich, in picturesque disorder, make the pleasing subaqual landscape effect of people.
Embodiment two:
Repairing place is about 2000 square meter waters in Changsha Cultural Park, and before repairing, water transparency is 0.06m, water quality It it is IV class water quality standard.Concretely comprise the following steps:
A, water quality detection: first the indices of water quality is detected;
B, remove weeds: waters will be processed and carry out weeds removing, and by geotextiles, process waters carried out the standards such as enclosure Standby work;
C, throw in photosynthetic bacteria: photosynthetic bacteria is thrown in into water body, input ratio be photosynthetic bacterium solution with water body than 1:250, wherein In photosynthetic bacterium solution, total number of bacteria is: 3-5 × 1010cfu/ml;
D, implant submerged plant: after photosynthetic bacteria repairs water body 25 days, by four kinds of submerged plant Herba vallisneriae Spiralis, hydrilla verticillata, Potarnogeton lucens and Ottelia acuminata (Gagnep.) Dandy flower be transplanted in Sediments, specially Herba vallisneriae Spiralis account for sum 70%, hydrilla verticillata 11%, light leaf eye Sub-dish 12%, Ottelia acuminata (Gagnep.) Dandy spend 7%, and method for transplanting is heavy hydroponics.
Throwing in the water body parameter before photosynthetic bacteria is transparency 0.06m, COD 89.2mg/L, BOD 16.4mg/L, TN 12.8mg/L, TP 0.9mg/L, after photosynthetic bacteria processes about 20d so that water transparency be adjusted to 0.3-0.8m, COD, BOD, TN and TP value reduces to 59.2mg/L, 11.6mg/L, 8.1mg/L and 0.5mg/L respectively.
After repairing water body half a year by the method for the present invention, submerged plant coverage rate reaches 82%;The cyanophyceae of water body is cut down Rate reaches 92%;Water body COD, BOD, TN and TP value reduces to 13.4mg/L, 3.6mg/L, 0.6mg/L and 0.05mg/L respectively, goes Except rate reaches 85-95%;Water transparency improves to more than 2m;Water quality reaches Group III water quality standard;Define and limpid seen The end, levels are rich, in picturesque disorder, make the pleasing subaqual landscape effect of people.
Embodiment three:
Repairing place is square meter waters, Changsha about 2500, and before repairing, water transparency is 0.07m, and water quality is IV class water gauge Accurate.Concretely comprise the following steps:
A, water quality detection: first the indices of the water quality in waters to be processed is detected;
B, removing weeds: process waters is carried out the preparations such as weeds removing;
C, throw in photosynthetic bacteria: photosynthetic bacteria is thrown in into water body, input ratio be photosynthetic bacterium solution with water body than 1:300, wherein In photosynthetic bacterium solution, total number of bacteria is: 3-5 × 1010cfu/ml;
D, implant submerged plant: after photosynthetic bacteria repairs water body 23 days, by four kinds of submerged plant Herba vallisneriae Spiralis, hydrilla verticillata, Potarnogeton lucens and Ottelia acuminata (Gagnep.) Dandy flower be transplanted in Sediments, specially Herba vallisneriae Spiralis account for sum 65%, hydrilla verticillata 15%, light leaf eye Sub-dish 12%, Ottelia acuminata (Gagnep.) Dandy spend 8%, and method for transplanting is carrier method for transplanting.
Throwing in the water body parameter before photosynthetic bacteria is transparency 0.07m, COD 80.5mg/L, BOD 15.6mg/L, TN 12.0mg/L, TP 0.8mg/L, processes after about 23 days through photosynthetic bacteria so that water transparency be adjusted to 0.3-0.8m, COD, BOD, TN and TP value reduces to 54.6mg/L, 11.8mg/L, 7.8mg/L and 0.45mg/L respectively.
After repairing water body half a year by the method for the present invention, submerged plant coverage rate reaches 83%;The cyanophyceae of water body is cut down Rate reaches 91%;Water body COD, BOD, TN and TP value reduces to 12.6mg/L, 3.4mg/L, 0.6mg/L and 0.04mg/L respectively, goes Except rate reaches 85-95%;Water transparency improves to more than 2m;Water quality reaches Group III water quality standard;Define and limpid seen The end, levels are rich, in picturesque disorder, make the pleasing subaqual landscape effect of people.

Claims (1)

1. the method utilizing Herba vallisneriae Spiralis to be main submerged plant and photosynthetic bacteria joint mapping subaqual landscape ecosystem, its feature It is that method step is as follows:
A, water quality detection: the first indices to water quality: water body total nitrogen, total phosphorus, COD, BOD and water transparency enter Row detection;
B, removing weeds: process waters is carried out in advance weeds and removes preparation;
C, input photosynthetic bacteria: being thrown in by photosynthetic bacteria into water body, input ratio is photosynthetic bacterium solution: water body=1:250-350, photosynthetic bacteria In liquid, total number of bacteria is 3-5 × 1010cfu/ml;
D, implantation submerged plant: after photosynthetic bacteria repairs water body 20-25 days, by four kinds of submerged plant Herba vallisneriae Spiralis, hydrilla verticillata, light Leaf Herba potamogetonis distincti and Ottelia acuminata (Gagnep.) Dandy flower are transplanted in Sediments, and concrete submerged plant consists of Herba vallisneriae Spiralis and accounts for the 60%-68% of quantity sum, wheel Leaf black algae 10%-15%, potarnogeton lucens 10%-15%, Ottelia acuminata (Gagnep.) Dandy flower 5%-10%, method for transplanting is cuttage or heavy hydroponics or carrier moves Hydroponics.
CN201410436933.5A 2014-08-30 2014-08-30 A kind of method utilizing Herba vallisneriae Spiralis to be main submerged plant and photosynthetic bacteria joint mapping subaqual landscape ecosystem CN104150604B (en)

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CN105981637A (en) * 2015-02-13 2016-10-05 上海太和水环境科技发展有限公司 Method of adding microorganisms for promoting growth of common andrographis herbs
CN105152347A (en) * 2015-09-28 2015-12-16 四川鑫淼环保科技有限责任公司 Building and management method for submerged vegetations in water of lakes
CN106927570B (en) * 2016-08-12 2020-09-01 上海水源地建设发展有限公司 Low-short flower-free tape grass-based plant-bacterium symbiotic underwater lawn

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11226593A (en) * 1998-02-16 1999-08-24 Shimizu Corp Water quality purification type floating island
CN101050043A (en) * 2007-05-11 2007-10-10 胡法 Ecological management method for water pollution
CN101885543A (en) * 2010-07-01 2010-11-17 毛晓敏 Method for effectively treating sewage by using both microbial cells and enzyme preparations
CN102432107A (en) * 2011-08-19 2012-05-02 上海水平衡环境科技发展有限公司 Biological integrated purification process for governing black and odorous river channels
CN102976492A (en) * 2012-11-22 2013-03-20 深圳市恒源浩科技有限公司 Water ecological purification system and method
CN103214099A (en) * 2013-05-07 2013-07-24 吕健 Ecological restoration and purification method for water of lake and pool wet land

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11226593A (en) * 1998-02-16 1999-08-24 Shimizu Corp Water quality purification type floating island
CN101050043A (en) * 2007-05-11 2007-10-10 胡法 Ecological management method for water pollution
CN101885543A (en) * 2010-07-01 2010-11-17 毛晓敏 Method for effectively treating sewage by using both microbial cells and enzyme preparations
CN102432107A (en) * 2011-08-19 2012-05-02 上海水平衡环境科技发展有限公司 Biological integrated purification process for governing black and odorous river channels
CN102976492A (en) * 2012-11-22 2013-03-20 深圳市恒源浩科技有限公司 Water ecological purification system and method
CN103214099A (en) * 2013-05-07 2013-07-24 吕健 Ecological restoration and purification method for water of lake and pool wet land

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