CN106922797A - A kind of natural biological freshness-preserving agent and its for fresh-keeping method for penaeus vannamei - Google Patents
A kind of natural biological freshness-preserving agent and its for fresh-keeping method for penaeus vannamei Download PDFInfo
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- CN106922797A CN106922797A CN201710186388.2A CN201710186388A CN106922797A CN 106922797 A CN106922797 A CN 106922797A CN 201710186388 A CN201710186388 A CN 201710186388A CN 106922797 A CN106922797 A CN 106922797A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/06—Freezing; Subsequent thawing; Cooling
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
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Abstract
The invention discloses a kind of natural biological freshness-preserving agent and its for fresh-keeping method for penaeus vannamei, it is made up of the component comprising following weight portion：10 25 parts of galangal flavone extract, 5 15 parts of waxberry dregs Polyphenols extract, 10 20 parts of bamboo vinegar, 5 10 parts of Tea Polyphenols, 35 parts of shitosan, 50 100 parts of water；Wherein, galangal flavone extract uses microwave radiation technology extracting technology, waxberry dregs Polyphenols extract to use ultrasonic extracting process, bamboo vinegar to be obtained after the vaporization liquid that bamboo wood high-temperature heating is decomposed is cooled down；Above-mentioned natural biological freshness-preserving agent is processed into the 10min of Penaeus Vannmei 5 by spray or immersion way, combination with ice-temperature preserves preservation under the conditions of 20 DEG C after taking-up；The natural biological freshness-preserving agent prepared using various pure natural plant ingredients compounded combinations, Synergistic, without antibiotic and conventional sterilization agent, component safety and environmental protection, with broad-spectrum antibacterial and fresh-keeping effect, extend the storage life and shelf life of prawn, with wide market application foreground.
Description
Technical field
The invention belongs to food processing technology field, and in particular to a kind of natural biological freshness-preserving agent and its right in vain for South America
The preservation method of shrimp.
Background technology
Penaeus Vannmei (Penaeus Vannamei) is the prawn kind that cultured area is maximum in the world, yield is most,
It is also unique Variety comprehensive of China's cultured prawn, yield maintains the growth rate of 7-8% every year, and nutritious, meat is fresh
U.S., it is deep to be liked by domestic and international consumer.But, substantial amounts of high activity polyphenol oxidase is contained in Penaeus Vannmei body, in shrimp
Body causes to produce blacking after harvesting death, has a strong impact on its edibility and economic worth.
With it is industrialized development and science and technology progress, engendered in terms of food preservation chemistry and biotechnology,
Preservation by low temperature, heating, controlled atmosphere preservation and the fresh-keeping preservation technology of novel physical etc. modernize preservation method；With aquatic products row
The links such as the development of industry, fresh-keeping processing, transport, storage and the consumption to whole aquatic products of aquatic products are more and more important, especially
It is with the security and the high requirement of freshness that the internationalization of aquatic products circulates and people originate to aquatic products.Biology is protected
Fresh dose is the antistaling agent for being extracted from animals and plants, microorganism or being obtained using modern biotechnology, particularly natural biological
The characteristic such as its good antioxidation activity of the antistaling agent origin cause of formation and bacteriostatic activity, safety non-toxic turns into the research heat of preservation of fishery
Point.
Galangal (Alpinia Officinarum Hance) is herbaceos perennial, originate in China Guangdong, Guangxi,
Hainan etc. saves, and has a small amount of plantation and wild distribution in Yunnan and Taiwan.Galangal contains substantial amounts of flavonoids, Diphenylheptane
The active skull cap components such as class, plant flavone has numerous physiologically actives such as analgesia, antibacterial, anti-inflammatory, antitumor, anti diar rhea.Bamboo vinegar
Liquid is nontoxic, the natural materials of safety, and itself has good inoxidizability and biocidal property, and to other bacteriostatic agents or sterilization
Agent has synergistic effect；Waxberry dregs are contained within a large amount of polyphenols, and such compound has antioxidation activity, antibacterial etc. biological special
Property.Although the preservation method of research prawn is many at present, it is applied to as antistaling agent there is presently no using above-mentioned natural materials
The fresh-keeping relevant report of Penaeus Vannmei.
Above-mentioned natural materials compounding is prepared natural biological freshness-preserving agent by the present invention, using pure natural plant ingredients, without anti-
Raw element and conventional sterilization agent, component safety and environmental protection, with broad-spectrum antimicrobial effect, effectively suppress Penaeus Vannmei in Cord blood
During microorganism growth, it is to avoid its is putrid and deteriorated, extend prawn storage life and shelf life.
The content of the invention
To overcome the drawbacks described above of prior art, it is an object of the invention to provide a kind of natural biological freshness-preserving agent.
The present invention also aims to provide above-mentioned natural biological freshness-preserving agent for fresh-keeping method for penaeus vannamei.
Above-mentioned purpose of the invention is achieved through the following technical solutions：
A kind of natural biological freshness-preserving agent, is made up of the component comprising following weight portion：Galangal flavone extract 10-25
Part, 5-15 parts of waxberry dregs Polyphenols extract, bamboo vinegar 10-20 parts, Tea Polyphenols 5-10 parts, shitosan 3-5 parts, water 50-100
Part.
Described galangal flavone extract uses microwave radiation technology extracting technology, including：Take galangal and Extraction solvent
Mix homogenate in refiner to extract；The material being homogenized after extracting is placed in microwave device and is extracted, filtered, and collect filtrate；
Filtrate crosses large pore resin absorption column, by ethanol elution, collects eluent, concentration, obtains final product galangal flavone extract.
Described microwave radiation technology extracting technology parameter includes：Galangal is 1 with Extraction solvent feed liquid mass volume ratio：5-
10, microwave power is 300-800W, is homogenized extraction time 20-30min, and the microwave digestion time is 4-6h, is extracted 1-3 times.
The Extraction solvent of described galangal flavone extract is ethanol or ethyl acetate, and concentration is 75wt%.
The antipathogenic composition of described galangal flavone extract include Galangin, Quercetin, kaempferol, pinocembrin,
Curcumin, protocatechuic acid.
The antibacterial circle diameter of described galangal flavone extract is 17.5-18.5mm.
Described waxberry dregs Polyphenols extract uses ultrasonic extracting process, and described process conditions include：Ultrasonic work(
Rate is 30-50W, and supersonic frequency is 20-40kHz, and feed liquid mass volume ratio is 1：4-6, extraction time is 30-60min, extracts temperature
It is 45-60 DEG C to spend, and is extracted 1-2 times.
The Extraction solvent of described waxberry dregs Polyphenols extract is selected from ethyl acetate, n-butanol, petroleum ether, water.
Described bamboo vinegar is obtained after the vaporization liquid that bamboo wood high-temperature heating is decomposed is cooled down.
Described chitosan solution concentration is 0.5-0.8g/100mL, and described Tea Polyphenols solution concentration is 0.5-1.2g/
100mL。
A kind of natural biological freshness-preserving agent is used for fresh-keeping method for penaeus vannamei, including：Will be according to above-mentioned composition and content
The natural biological freshness-preserving agent of compounding by spray or immersion way process Penaeus Vannmei 5-10min, after taking-up combination with ice-temperature-
Preserved under the conditions of 2-0 DEG C and preserved.
The invention has the advantages that：
The natural biological freshness-preserving agent that the present invention is prepared using various pure natural plant ingredients compounded combinations, Synergistic, no
Containing antibiotic and conventional sterilization agent, component safety and environmental protection, with broad-spectrum antimicrobial effect；In addition, the present invention is fresh-keeping by natural biological
The storage of agent combination with ice-temperature is fresh-keeping to Penaeus Vannmei, and antibacterial and fresh-keeping effect is good, and natural bacteriostatic agent composition therein is effectively
Suppress Penaeus Vannmei microorganism during Cord blood growth, it is to avoid its is putrid and deteriorated, extend prawn storage life and
Shelf life, with wide market application foreground.
Specific embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Embodiment 1
A kind of natural biological freshness-preserving agent, is made up of the component comprising following weight portion：25 parts of galangal flavone extract,
15 parts of waxberry dregs Polyphenols extract, 20 parts of bamboo vinegar, 10 parts of Tea Polyphenols, 5 parts of shitosan, 100 parts of water.
Wherein, galangal flavone extract use microwave radiation technology extracting technology for：Feeding liquid mass volume ratio is 1：10
Galangal and concentration mix homogenate extraction 30min in refiner for the ethyl acetate solvent of 75wt%；After homogenate is extracted
Material be placed in the microwave device that microwave power is 800W and extract 6h, filtered after extracting 3 times and collect filtrate；Filtrate crosses macropore
Adsorption resin column, by ethanol elution, collects eluent, concentration, obtains final product galangal flavone extract；Galangal flavonoids is carried
The antipathogenic composition for taking thing includes Galangin, Quercetin, kaempferol, pinocembrin, curcumin, protocatechuic acid；Galangal flavonoids
The antibacterial circle diameter of extract is 18.5mm.
Waxberry dregs Polyphenols extract use ultrasonic extracting process condition for：Ultrasonic power is 50W, and supersonic frequency is
40kHz, feed liquid mass volume ratio is 1：6,60min is extracted, Extracting temperature is 60 DEG C, is extracted 2 times；Extraction solvent is n-butanol.
Bamboo vinegar is obtained after the vaporization liquid that bamboo wood high-temperature heating is decomposed is cooled down, and chitosan solution concentration is 0.8g/
100mL, Tea Polyphenols solution concentration is 1.2g/100mL.
A kind of natural biological freshness-preserving agent is for fresh-keeping method for penaeus vannamei：To be compounded according to above-mentioned composition and content
Natural biological freshness-preserving agent by spray or immersion way process Penaeus Vannmei 10min, combination with ice-temperature is in 0 DEG C of condition after taking-up
Lower storage is preserved.
Embodiment 2
A kind of natural biological freshness-preserving agent, is made up of the component comprising following weight portion：15 parts of galangal flavone extract,
10 parts of waxberry dregs Polyphenols extract, 15 parts of bamboo vinegar, 7 parts of Tea Polyphenols, 4 parts of shitosan, 80 parts of water.
Wherein, galangal flavone extract use microwave radiation technology extracting technology for：Feeding liquid mass volume ratio is 1：6
Galangal extracts 25min with concentration for the alcohol solvent of 75wt% mixes homogenate in refiner；The material after extracting will be homogenized
It is placed in the microwave device that microwave power is 500W and extracts 5h, is filtered after extracting 2 times and collect filtrate；Filtrate crosses macroporous absorption tree
Fat post, by ethanol elution, collects eluent, concentration, obtains final product galangal flavone extract；Galangal flavone extract
Antipathogenic composition includes Galangin, Quercetin, kaempferol, pinocembrin, curcumin, protocatechuic acid；Galangal flavone extract
Antibacterial circle diameter be 18.0mm.
Waxberry dregs Polyphenols extract use ultrasonic extracting process condition for：Ultrasonic power is 40W, and supersonic frequency is
30kHz, feed liquid mass volume ratio is 1：5,40min is extracted, Extracting temperature is 50 DEG C, is extracted 1 time；Extraction solvent is petroleum ether.
Bamboo vinegar is obtained after the vaporization liquid that bamboo wood high-temperature heating is decomposed is cooled down, and chitosan solution concentration is 0.6g/
100mL, Tea Polyphenols solution concentration is 0.8g/100mL.
A kind of natural biological freshness-preserving agent is for fresh-keeping method for penaeus vannamei：To be compounded according to above-mentioned composition and content
Natural biological freshness-preserving agent by spray or immersion way process Penaeus Vannmei 7min, combination with ice-temperature is in -1 DEG C of condition after taking-up
Lower storage is preserved.
Embodiment 3
A kind of natural biological freshness-preserving agent, is made up of the component comprising following weight portion：20 parts of galangal flavone extract,
12 parts of waxberry dregs Polyphenols extract, 18 parts of bamboo vinegar, 8 parts of Tea Polyphenols, 4 parts of shitosan, 90 parts of water.
Wherein, galangal flavone extract use microwave radiation technology extracting technology for：Feeding liquid mass volume ratio is 1：8
Galangal extracts 28min with concentration for the ethyl acetate Extraction solvent of 75wt% mixes homogenate in refiner；Homogenate is extracted
Material afterwards is placed in the microwave device that microwave power is 600W and extracts 6h, is filtered after extracting 2 times and collects filtrate；Filtrate is excessive
Macroporous adsorbent resin post, by ethanol elution, collects eluent, concentration, obtains final product galangal flavone extract；Galangal flavonoids
The antipathogenic composition of extract includes Galangin, Quercetin, kaempferol, pinocembrin, curcumin, protocatechuic acid；Galangal flavones
The antibacterial circle diameter of class extract is 18.2mm.
Waxberry dregs Polyphenols extract use ultrasonic extracting process condition for：Ultrasonic power is 45W, and supersonic frequency is
35kHz, feed liquid mass volume ratio is 1：5, extraction time is 50min, and Extracting temperature is 60 DEG C, is extracted 2 times；Extraction solvent is
Water.
Bamboo vinegar is obtained after the vaporization liquid that bamboo wood high-temperature heating is decomposed is cooled down, and chitosan solution concentration is 0.7g/
100mL, Tea Polyphenols solution concentration is 1.0g/100mL.
A kind of natural biological freshness-preserving agent is for fresh-keeping method for penaeus vannamei：To be compounded according to above-mentioned composition and content
Natural biological freshness-preserving agent by spray or immersion way process Penaeus Vannmei 5-10min, combination with ice-temperature is in -2-0 after taking-up
Preserved under the conditions of DEG C and preserved.
Embodiment 4
A kind of natural biological freshness-preserving agent, is made up of the component comprising following weight portion：10 parts of galangal flavone extract,
5 parts of waxberry dregs Polyphenols extract, 10 parts of bamboo vinegar, 5 parts of Tea Polyphenols, 3 parts of shitosan, 50 parts of water.
Wherein, galangal flavone extract use microwave radiation technology extracting technology for：Feeding liquid mass volume ratio is 1：5
Galangal extracts 20min with concentration for the alcohol solvent of 75wt% mixes homogenate in refiner；The material after extracting will be homogenized
It is placed in the microwave device that microwave power is 300W and extracts 4h, is filtered after extracting 1 time and collect filtrate；Filtrate crosses macroporous absorption tree
Fat post, by ethanol elution, collects eluent, concentration, obtains final product galangal flavone extract；Galangal flavone extract
Antipathogenic composition includes Galangin, Quercetin, kaempferol, pinocembrin, curcumin, protocatechuic acid；Galangal flavone extract
Antibacterial circle diameter be 17.5mm.
Waxberry dregs Polyphenols extract use ultrasonic extracting process condition for：Ultrasonic power is 30W, and supersonic frequency is
20kHz, feed liquid mass volume ratio is 1：4,30min is extracted, Extracting temperature is 45 DEG C, is extracted 1 time；Extraction solvent is acetic acid second
Ester.
Bamboo vinegar is obtained after the vaporization liquid that bamboo wood high-temperature heating is decomposed is cooled down, and chitosan solution concentration is 0.5g/
100mL, Tea Polyphenols solution concentration is 0.5g/100mL.
A kind of natural biological freshness-preserving agent is for fresh-keeping method for penaeus vannamei：To be compounded according to above-mentioned composition and content
Natural biological freshness-preserving agent by spray or immersion way process Penaeus Vannmei 5min, combination with ice-temperature is in -2 DEG C of conditions after taking-up
Lower storage is preserved.
Although the present invention is described in detail above to have used general explanation and specific embodiment, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (10)
1. a kind of natural biological freshness-preserving agent, it is characterised in that be made up of the component comprising following weight portion：Galangal flavonoids is carried
Take thing 10-25 parts, 5-15 parts of waxberry dregs Polyphenols extract, bamboo vinegar 10-20 parts, Tea Polyphenols 5-10 parts, shitosan 3-5 parts,
Water 50-100 parts.
2. a kind of natural biological freshness-preserving agent according to claim 1, it is characterised in that described galangal flavonoids is extracted
Thing uses microwave radiation technology extracting technology, including：Take galangal and mix homogenate extraction in refiner with Extraction solvent；Homogenate is carried
Material after taking is placed in microwave device and extracts, filters, and collects filtrate；Filtrate crosses large pore resin absorption column, is washed by ethanol
It is de-, eluent, concentration are collected, obtain final product galangal flavone extract.
3. a kind of natural biological freshness-preserving agent according to claim 2, it is characterised in that described microwave radiation technology extracting technology
Parameter includes：Galangal is 1 with Extraction solvent feed liquid mass volume ratio：5-10, microwave power is 300-800W, when homogenate is extracted
Between 20-30min, the microwave digestion time be 4-6h, extract 1-3 times；The Extraction solvent of described galangal flavone extract is
Ethanol or ethyl acetate, concentration are 75wt%.
4. a kind of natural biological freshness-preserving agent according to claim 1, it is characterised in that described galangal flavonoids is extracted
The antipathogenic composition of thing includes Galangin, Quercetin, kaempferol, pinocembrin, curcumin, protocatechuic acid.
5. a kind of natural biological freshness-preserving agent according to claim 1, it is characterised in that described galangal flavonoids is extracted
The antibacterial circle diameter of thing is 17.5-18.5mm.
6. a kind of natural biological freshness-preserving agent according to claim 1, it is characterised in that described waxberry dregs Polyphenols is extracted
Thing uses ultrasonic extracting process, and described process conditions include：Ultrasonic power is 30-50W, and supersonic frequency is 20-40kHz,
Feed liquid mass volume ratio is 1：4-6, extraction time is 30-60min, and Extracting temperature is 45-60 DEG C, is extracted 1-2 times.
7. a kind of natural biological freshness-preserving agent according to claim 1, it is characterised in that described waxberry dregs Polyphenols is extracted
The Extraction solvent of thing is selected from ethyl acetate, n-butanol, petroleum ether, water.
8. a kind of natural biological freshness-preserving agent according to claim 1, it is characterised in that described bamboo vinegar is high by bamboo wood
Obtained after the vaporization liquid cooling of warm heat resolve.
9. a kind of natural biological freshness-preserving agent according to claim 1, it is characterised in that described chitosan solution concentration is
0.5-0.8g/100mL, described Tea Polyphenols solution concentration is 0.5-1.2g/100mL.
10. a kind of natural biological freshness-preserving agent according to claim any one of 1-9 is used for fresh-keeping method for penaeus vannamei,
It is characterised in that it includes：The natural biological freshness-preserving agent that will be compounded according to above-mentioned composition and content passes through at spray or immersion way
Reason Penaeus Vannmei 5-10min, combination with ice-temperature preserves preservation under the conditions of -2-0 DEG C after taking-up.
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| CN109265802A (en) * | 2018-09-07 | 2019-01-25 | 中国科学院烟台海岸带研究所 | A kind of antimicrobial preservative film for marine product |
| CN109362865A (en) * | 2018-06-25 | 2019-02-22 | 浙江省农业科学院 | A kind of waxberry dregs extract composite antistaling agent and its application on the cold fresh meat of giant salamander |
| CN110122545A (en) * | 2019-06-12 | 2019-08-16 | 广西壮族自治区水产科学研究院 | A kind of prawn preservation method |
| WO2019238376A1 (en) * | 2018-06-14 | 2019-12-19 | Unilever Plc | Foodstuff comprising a preservative |
| CN110915886A (en) * | 2019-10-14 | 2020-03-27 | 盐城工学院 | Aquatic product composite biological preservative and preparation method and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2019238376A1 (en) * | 2018-06-14 | 2019-12-19 | Unilever Plc | Foodstuff comprising a preservative |
| CN109362865A (en) * | 2018-06-25 | 2019-02-22 | 浙江省农业科学院 | A kind of waxberry dregs extract composite antistaling agent and its application on the cold fresh meat of giant salamander |
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| CN110915886A (en) * | 2019-10-14 | 2020-03-27 | 盐城工学院 | Aquatic product composite biological preservative and preparation method and application thereof |
| CN110915886B (en) * | 2019-10-14 | 2023-07-25 | 盐城工学院 | Composite biological preservative for aquatic products and preparation method and application thereof |
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Application publication date: 20170707 |
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