CN106916834B - Biosynthetic gene cluster of compounds and application thereof - Google Patents

Biosynthetic gene cluster of compounds and application thereof Download PDF

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CN106916834B
CN106916834B CN201510992001.3A CN201510992001A CN106916834B CN 106916834 B CN106916834 B CN 106916834B CN 201510992001 A CN201510992001 A CN 201510992001A CN 106916834 B CN106916834 B CN 106916834B
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刘然
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Wuhan Hesheng Technology Co ltd
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Abstract

The invention discloses a biosynthetic gene cluster of a compound and application thereof. Wherein the biosynthetic gene cluster of the compound comprises: the I type linear polyketide synthase gene module comprises: 2a1 gene, 2a2 gene, 2A3 gene, 2a4 gene, 2a5 gene, 2a6 gene, 2a7 gene, 2A8 gene, 2a9 gene, 2a10 gene and 2a11 gene; a glycosyl synthesis-associated gene module comprising: 2G1 gene, 2G2 gene, 2G3 gene, 2G4 gene, 2G5 gene, 2G6 gene, 2G7 gene, 2G8 gene and 2M gene; and a modifier gene module comprising: 2O gene, 2I gene, 2E gene, 2P1 gene and 2P2 gene.

Description

Biosynthetic gene cluster of compounds and application thereof
Technical Field
The invention relates to the field of bioengineering, in particular to a biosynthetic gene cluster of a J1001-2 compound and application thereof, and more particularly relates to a biosynthetic gene cluster of a J1001-2 compound, a microorganism and a method for preparing the J1001-2 compound.
Background
Polyketides are one of the most abundant groups of natural products, and are widely applied to the fields of medical use, veterinary use, agriculture and the like due to the great medicinal value of the polyketides. Such as the antibiotic erythromycin, the antiparasitic antibiotic abamectin, etc. Polyketides are formed under the catalysis of polyketide synthase (PKS), and gene clusters assembled in a modular form determine the structure and thus the biological activity of the polyketides, so that the gene cluster of a new compound has great significance for the subsequent research.
With the increasing severity of the drug resistance problem of pathogenic bacteria, the clinical need for new antibiotics is more urgent. In order to deal with the situation, people reform the existing antibiotics on one hand and accelerate the step of screening novel antibiotics on the other hand. The J1001-2 compound is a recently discovered antibiotic and has good bacteriostatic activity.
To date, no study related to the biosynthesis gene cluster of the J1001-2 compound has been reported.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art. To this end, it is an object of the present invention to propose a biosynthetic gene cluster of antibiotic-J1001-2 compounds having antibacterial activity.
According to one aspect of the present invention, the present invention provides a biosynthetic gene cluster of a J1001-2 compound, wherein the J1001-2 compound has the following structure,
Figure BDA0000889457240000011
according to an embodiment of the invention, the biosynthetic gene cluster comprises:
the I type linear polyketide synthase gene module comprises: 2a1 gene, 2a2 gene, 2A3 gene, 2a4 gene, 2a5 gene, 2a6 gene, 2a7 gene, 2A8 gene, 2a9 gene, 2a10 gene and 2a11 gene;
a glycosyl synthesis-associated gene module comprising: 2G1 gene, 2G2 gene, 2G3 gene, 2G4 gene, 2G5 gene, 2G6 gene, 2G7 gene, 2G8 gene and 2M gene; and
a modifier gene module comprising: 2O gene, 2I gene, 2E gene, 2P1 gene and 2P2 gene.
The inventors have surprisingly found that the biosynthetic gene cluster of the J1001-2 compound of the examples of the invention can control the biosynthesis of the J1001-2 compound.
According to an embodiment of the invention, the 2a1 gene encodes SEQ ID NO: 1; the 2A2 gene encodes SEQ ID NO: 2; the 2A3 gene encodes SEQ ID NO: 3; the 2A4 gene encodes SEQ ID NO: 4; the 2A5 gene encodes SEQ ID NO: 5; the 2A6 gene encodes SEQ ID NO: 6; the 2A7 gene encodes SEQ ID NO: 7; the 2A8 gene encodes SEQ ID NO: 8; the 2A9 gene encodes SEQ ID NO: 9; the 2A10 gene encodes SEQ ID NO: 10; the 2A11 gene encodes SEQ ID NO: 11, or a pharmaceutically acceptable salt thereof.
According to an embodiment of the invention, the 2G1 gene encodes SEQ ID NO: 12; the 2G2 gene encodes SEQ ID NO: 13; the 2G3 gene encodes SEQ ID NO: 14; the 2G4 gene encodes SEQ ID NO: 15; the 2G5 gene encodes SEQ ID NO: 16; the 2G6 gene encodes SEQ ID NO: 17; the 2G7 gene encodes SEQ ID NO: 18; the 2G8 gene encodes SEQ ID NO: 19; the 2M gene encodes SEQ ID NO: 20, or a pharmaceutically acceptable salt thereof.
According to an embodiment of the invention, the 2O gene encodes SEQ ID NO: 21; the 2I gene encodes SEQ ID NO: 22; the 2E gene encodes SEQ ID NO: 23; the 2P1 gene encodes SEQ ID NO: 24; the 2P2 gene encodes SEQ ID NO: 25, or a pharmaceutically acceptable salt thereof.
According to an embodiment of the present invention, the biosynthetic gene cluster further comprises: a regulatory gene module comprising: 2R1 gene, 2R2 gene, 2R3 gene, 2R4 gene, 2T1 gene, 2T2 gene, 2T3 gene, 2T4 gene and 2T5 gene.
According to an embodiment of the invention, the 2R1 gene encodes SEQ ID NO: 26; the 2R2 gene encodes SEQ ID NO: 27, or a pharmaceutically acceptable salt thereof; the 2R3 gene encodes SEQ ID NO: 28; the 2R4 gene encodes SEQ ID NO: 29; the 2T1 gene encodes SEQ ID NO: 30; the 2T2 gene encodes SEQ ID NO: 31; the 2T3 gene encodes SEQ ID NO: 32; the 2T4 gene encodes SEQ ID NO: 33; the 2T5 gene encodes SEQ ID NO: 34, or a pharmaceutically acceptable salt thereof.
According to the embodiment of the present invention, along the 3 '-5' end of the gene, the 2T2 gene, the 2T1 gene, the 2G8 gene, the 2G7 gene, the 2G6 gene, the 2R4 gene, the 2R3 gene, the 2P1 gene, the 2A8 gene, the 2A11 gene, the 2P 11 gene, the 2I gene, the 2O gene, the 2A11 gene, the 2E gene, the 2A11 gene, the 2G 11 gene, the 2M gene, the 2A11 gene, the 2R 11 gene, the 2T 11 gene, the 2G 11 gene and the 2A11 gene are connected in series.
According to an embodiment of the invention, the biosynthetic gene cluster has the amino acid sequence of SEQ ID NO: 35.
According to another aspect of the present invention, there is provided a microorganism. According to an embodiment of the present invention, the microorganism has the aforementioned biosynthetic gene cluster. The inventors have surprisingly found that the J1001-2 compound can be synthesized efficiently, simply and at low cost by performing a fermentation treatment using the microorganism.
According to an embodiment of the invention, the microorganism is streptomyces.
According to yet another aspect of the present invention, there is provided a process for preparing a J1001-2 compound. According to an embodiment of the invention, the method comprises: carrying out fermentation treatment by using the microorganisms so as to obtain fermented microorganisms; carrying out wall breaking treatment on the fermented microorganisms to obtain a solution containing the J1001-2 compound; and purifying the J1001-2 compound from the solution containing the J1001-2 compound so as to obtain a pure product of the J1001-2 compound.
According to the method for producing the J1001-2 compound of the embodiment of the present invention, the J1001-2 compound can be efficiently synthesized by performing the fermentation treatment using the aforementioned microorganism. Then, the pure J1001-2 compound can be obtained efficiently, simply and at low cost through microbial wall breaking treatment and separation and purification treatment.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 shows a schematic diagram of the sequence of biosynthetic gene cluster for compound J1001-2 according to one embodiment of the present invention;
FIG. 2 shows a schematic flow diagram of a process for preparing a J1001-2 compound, according to one embodiment of the present invention;
FIG. 3 shows a schematic flow diagram of the biosynthetic pathway of the J1001-2 compound according to one embodiment of the present invention;
FIG. 4 shows a schematic map of a J1001-2 compound according to one embodiment of the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, which are for convenience of description of the present invention only and do not require that the present invention must be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
It should be noted that the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. Further, in the description of the present invention, "a plurality" means two or more unless otherwise specified.
According to one aspect of the present invention, there is provided a biosynthetic gene cluster of a J1001-2 compound. According to an embodiment of the invention, the biosynthetic gene cluster comprises:
the I type linear polyketide synthase gene module comprises: 2a1 gene, 2a2 gene, 2A3 gene, 2a4 gene, 2a5 gene, 2a6 gene, 2a7 gene, 2A8 gene, 2a9 gene, 2a10 gene and 2a11 gene;
a gene module associated with glycosyl synthesis, the gene module comprising: 2G1 gene, 2G2 gene, 2G3 gene, 2G4 gene, 2G5 gene, 2G6 gene, 2G7 gene, 2G8 gene and 2M gene; and
a modifier gene module comprising: 2O gene, 2I gene, 2E gene, 2P1 gene and 2P2 gene.
The inventors have surprisingly found that the biosynthetic gene cluster of the J1001-2 compound of the examples of the invention can control the biosynthesis of the J1001-2 compound.
According to an embodiment of the present invention, the biosynthetic gene cluster (also referred to as simply "gene cluster" in the present invention) is a biosynthetic gene cluster of streptomyces having the sequence of seq id NO: 35, specifically as follows:
ATGGCCGTGAAAGGTCTCAGAAAAGCGATCGCACGATACGGGGCACCGACCGCGGGATGCGTCGTCGCGCTGCTCGCGCTCACCGGATGTCTCGGCTCGCCCGGCGCGCCGGAACGGCGGGGCGGGCTGTGGGTGAACCCGGACAACCCGGCGGCCCGGCACGCCCGTGACCAGCGGCGCGCCGGCCGGTACGCGGACGCGCGGCTCATGCGGCGGATCGCCGACGAGCCGCTGGCCGAATGGCTCGGCCCGAATCCGCGCGACCGGGTCAGGCTCGTCACCGAATCCGCCGCGCGGGAACACCGTACGCCGGTGCTGGTCGCTTATCACATTCCGTACCGGGACTGCGGGCATTACTCCGCGGGAGGGGCGAGGAACGACAGGGAATACCTTGACTGGATCCGGGAAATCGTCTCCGGAATCGGCGATCGAAAGGCGATTGTCGTATTGGAGCCCGACGCGGTGGCCCAGGTGGTCGACGGCTGTGTCCCCGAGCGGCTGCGGGGCCCGCGGCTGGCCCTGCTGCGCGACGCCGTCGCCATGCTCTCCGCGCCGCCCGGCACCCAGGTGTATCTCGACGCGGGGAACCCGGGGTGGATCCGGAACACCCAGCGGCTGGTGGAGCCGCTCTGGGAGAGCGGCATCGAGCAGGCGGACGGCTTCTCGCTGAACGTCTCCAACTTCCAGCCGACGCAATTGACCCGCGCCTACGGCCACCGGCTGTCGACCCAGCTCGGCGGCGCCCACTTCATCATCGACACCAGCCGCAACGGCAACGGCCCGCTCACCCGCGGCGGGGAGGGCGGGGCCGAAGACGCCAAGGGCGGCGCGCGGGACGACGCCAAGGGCGGCGGCGGAAGCGCGGGACATCCCGGCACCGGCGCGGGCACCCGCCATCGCGTCGTCGGCGGCGAGGAGAACTGGTGCAACCCCCCGGGCCGGGCGCTGGGCGAGCCCCCGAGCACCCGCACCGGCGACCCCTCCATCGACGCCTATCTGTGGATCAAGCGCCCCGGCGAGTCCGACGGGACCTGCAACGGCGGCCCCCCGGCCGGCCGTTGGTGGACGGAGTACGCCCTCGACCTGGCCCGCAACGCCAAGGCGCGCCACAGGTGACGGCGGGCGGGAGCGGGTGACGGCAGACGGGCGTGGATGAGGGCACGGGCCGTCAGCCCGCCCCGGCGTGGGCCTCGGCGCGCGCCTCCGCGGGCGCCTCGGCGCGGGCCGTCGGCACCGCCGCGGCGGTGCGCCAGGTGCGCCGCGCCAGCAACAGCCCGCCCACCCAGATGGCCATCGGCGCCAGGCACAGCCCCACCGCCAGCAGCGCCCAGGTGCGCATCGCCGCGTGGGTGTGCCCCAGGAACAGCGAGGCGACCAGCGAGCCGCCGAACACCGCGGCCCACACCAGATGGATACGGAAGGTGGACAGCTGGTCGGCGCTGGAGGAGTCGCCCTTCGGCGTGACCACGAACCGGCTTCTGCGCCGCAGCAGCGCGTCGCACAGCGACTTGGCGTAGATCGGGGCGGACAGCGCGGACAGCAGCATCCCCGCCACCCCGCCGGAGCCCTCCGGCTCGTGAGGGGAGACGTTGTGCCGCCGGTTCCACACGTACAGCCCGATCTGGAGCGCCGCGGCGTCGCTGTAGAGCATCATCCACATCTCCGCCGGGATGTGGATCCCGGAGGCGCCCAGCACCAGGAACAGCGCGCAGGACAGCGCCGACAGCATCCAGTTGAGCGCGGTCATCGGGTAGTACGCGATCATCAGCGAGTAGTTGAGCAGCTTCCCCGGCGACAGTGTGAACGGCGCCTTCCAGAACTGCTTGAGCACCGTCTCGTAGGTGCCCCGGCTCCAGCGCAGCTGCTGGGTGAAGAAGTCCGTCCAGGTGCTCGGGCCCTCCCCGACGGCCAGCACGTCCGGGGTGTAGACCGAGCGCCACTTCTTCCTGGTGACCGGGTTGCGGCGGCGGTGCAGCTCGAAGCCGGTGGCCATGTCCTCGGTGATCGAGTCGTACAGCCCGCCGATGCTCCGCAGCGCGGCGATGCGCACCGCGTTGTTGGTCCCGACGAACATGGGGCCGCCATACGCGTTTCCCGCCCGCTGGATCAGCGCGTGGAACAGGAACTGCTGGCTCTCGGCCGCTTTGGTGACCGCCGTGTCGTAATTGCCGTACACCTGCGGGCCGACCACAAAGGCGATATCCGGGTCGCGGAAGTAACCCATCATCCGCTCGAGGAAGTTGGGCAGCGGAACGTGATCGGTGTCCACGCAGGCCATGAATTCATAGTCGTCGCCATGGGCGTCCAGCCAGGCGTTGTAATTCCCGTGCTTGGTCTTCGCACGAAAGGGCCCCTTCGGCTGATTCCACTTCTCGACACCCTTGCGGGAGAAATGCCGGACGCCGAGCCGCGCGCACAGCTCCTTGACCTCGTCGTCGTTCCCCTCGTCCAGCAGCCATACGTCGAGCCACCCGGCGTGCCTGACCCGCATCGCCCCCTCCAGCGTCGCCCGGACCATCTCGATCGGCTCCTTGCCGGGCACGAAGCTGGTCAGAAACGCCACCCGGGTCCCCGGCTCGGCGGTCACCGGCACCGGGTCCCGGGCCACCAGCGTGGCGTGCGCGTTGGAGACCACGGTGAACAGGCGGAACGCCTCGATCAGCGCGATCGAGAGCAGCATCGTGGTGTCCGCCGCCGTCTCCCAGGCCGCCACATAGTCGCGGTGCACCCAGTGCTGCGGCTGGAGCAGCCACAGCAGCAGCAGCCCCGAACACACCGGCGCCGCGCACAGCAGCAGCGCCGCGCGGACGCGGTGCGGCTCCTGCGCCAG CAGGCTGCGGCAGCGCACCCGGTACGGGCGGCCGGGCGGCGGCTGGGTCAGCGGGCCCGCCAGTTGGCTGTACCGCTTGTACTCGTACGCCGCCGCGGGGCCCCGGCGGCGGGAGGTCGTCCCATGGTGCGCGGGCTGTGCGGGGCGCGGCGCGGCGCGGGGCGTGGCACCCGGCTCGGAAGCGTCTCTGACGGGCGTTGGTGCAGACATCTGGGCACTCCCGCGGGTCGCCTGGGACAAAATATCGCGCGGCGATGACCCGGACGGCTTACATTCGTCAGGTCATCGCGCGAGATGAGAAGGTAACAATCCCATGGGGAATGACTGTGCAAATCGGATTTTCGGCGCGCCTTGGCGCGCCCCGGTGCTCCCCGTCGCTTCCCGGCGCTGCCCGTCGTATTGGGCGGACCGCCACTCCGACGGCCTATAGCCGCGCGTCTTACGTGATTTACATCCCGACGTCTGTCATGGTCGGATGCGATTCGGCGTGCATGTTTCGACCGCGCGATCCGGGCTGCGTTTCGGCCGCGCGTTTCGGCTGCCGGGTTCGGCATGTGTGAGGAAGCCCGCGCCATGGGGCGGGCGCGACAGGAAAGATGGGGCCCGGTCGCCCGATGAGAGTGCTGCACATCATCTCGCGCCTCGGCGCTGGTGGCGCCGAGCAGCAACTGCGGCTGCTGCTGCGCCACCTGCCCGCCCGCTGTGACGTCCTCACCCTGTCCGCCCCCGGGGCCGCCGGCCACGGCATCCGGGCGGACGGGGTGCCCGTCACCCACCTGCCCCTGCGCGGCCGCGACCCGATGGCTTCGGCCCGGCTGGCGCGGTTCATCCGCGGCGGCGGCTACGACCTGGTCCACACCCATCTCCACCGGGCCTGCGTATACGGCCGGATCGCCGCCCGGCTGGCGGGCGTCGGCGCCGTGATCGCCACCGAACACAGCCTGGGCGACCGCCACATCGACGGCCGCCCGCACACCGTCGGCACCCGTGCCCGCTACCTCGCCGCCGAGCGCCTGGGCTCGGCGACCGTCGCCGTCTCGGCCGCCGTCGCCGACCGGCTCCGCGCCTGGGGCGTCGCCAACCACCGCATCCACCTGGTGCCCAACGGCCTGGACGCCCGCCACTTCCACTACACCCCGGCCGCCCGCGCCGCCGCCCGCGCCCGCCTCGGCATCCCCGCACATGCCTTCGTCGTCGGCGGGGTGGGCCGGCTGATGGCGGCCAAGCGCTTCGACGTGGCGCTGCGGGCCGTCGCCGAGGTGCCCGGGGCCCGGCTGCTGCTGGTCGGCGACGGTCCCGAGCGCACCGCGCTGCACGACCTGGCCGCCTGGCTCGGGGTGGCCGACCGGGTCCGGCTGCTCGGCGAGCGCGACGGGGCGGTGGCCGGGACGGGGGACCGGCCCGCCGACATCCCCGGGCTGCTGGCCGCCATGGACGTCTTCGTCGCGCCATCCGCGGAGGAGGCGTTCGGCATCGCCGCCGTGGAGGCCCTCGCGGCCGGGCTGCCGGTGCTCTACACCGTCTGCCCCGCCCTCGACGAACTGCCGCCCGGCGCCGCCCCCGGCGCCCACCGCATCGGCCCCGGCGCCGCCGCCCTGGCCGGCGCCCTGCGCGAATCCGCCGCCGCCGGCCCCGTACGGTTCCCGGTGCCCGCCGCCGTCGAGCGCTACGACATCGTCCGCACCGCCCGCCGGCTGGCGCTGCTGTACGAACGCTCCGTCAGCTGCGGGCCCGACGGCTGCGCCCGCGGCCCCGCCGTCACCCTGCGCTGGCCGCGGAGCGGTGCTCCTGCGGGCTGACCCCGCGCACCCGCTTGAAGGCCGAGCTGAGCGCGTACGGGCTGCCGTAGCCGACCCGCCGGGCCACCGCCCCGAGGGTCGCGTCCGGCTCCCGCAGCAGATCGGCGGCCAGCGTCAGCCGCCAGCCGGTCAGATACGCCATGGGCGGCTCGCCGACCAGCTCGGTGAAGCGCCGGGCCAGCGCCGCCCGGGAGACCCCGGTCCGCTCGGCCAGCAGGGCCACCGTCCACGGATGGGCCGGATGGTTCTGAAGCAGCCGCAGGGCGCGGCCCACCACCGGGTCGCCCAGCGCCCGATACCAGGCCGGGGCCCGCGCCTCCGGCCGGGCGAACCACGCCCGCAGCACGGAGATCAGCACCAGGTCGAGCAGCCGGTCCAGCACGGCCGTCTGGCCCGCCTCGTCCTTGACGATCTCCTCGCCCAGCCACGACAACAACGGTGAGCCGCACTCCTCGCCCGGCAGCACCAGCAGCGGCGGCAGCGCGTCCAGCAGCCGCCGGCTGATCTCCCCGTCCATCCGGTACGTACCGACCAGCATCGTCGCCGAGCCGTGCTCGCTGTTGCCCCAGGTGCGCACCCCCAGCGCCATCCGCTCGGCCAGATCCTCGCCCTCGGCGGTGCGGCACACCTCGCCGGGCAGGATCCAGGCCTGCGGCCTGGTGGCCGGATCATCGGCCACCGTGTACGGGTCGGGGCCGCGCACGATCGCGACGTCCCCCGGAACCAGCCGCACCCCTTTTTCCGGGTCGCCACCCTCCGGGACCACCCATGCCTCGCCGCGCGCCACGGTCATCACACACAGCGGGGCCCGGTCCTCGATCCGCACGGACCAGGGCGGTTCCATGACCATACGGAGCAGAAACGCCCCCCGGGCTCTCGGCCCGTCCAGCAGGCCGGCCAGTGGATCCATGGCGCCCGCTACCGCCGTTCGAGGGCGGCCGTGGAGCCCCGTACCACCAGATGCGTCGGCAACACCCGCTGCGGGGGCTGCTCGCCCTCGGCCGCGGGGGTGGCCAGCATGCGGAACAGCAGATCGACGGCGGCCCGCGCGCCGTGCGCCTTGGGCACCGAGACCGTGGTCAGCGGCGGCGCCGCCATCCCGGCCAGGGCGATGTCGTCGCAGCCCACCACGCTCATCTCGTCCGGCACCCGCACCCCCCGCACCGCGAACCTGCTCATCAGACCGAACGCGACGATGTCGTCGAACGCCAGCACCGCGCTCGCCCCGCTGGCCAGCACCACATCGGCGGCCCCGGTGACCCCGCCGGGGGTGCCGATGTGATGCGCCACGCTGCCCACCTGCACGATCTCCACCAGCCCGGACTCGGCCACCGCCGCCACCCCGGCCGCCCGCTCCCGCGCGGCCCACGAGCTGCGCGGCCCTCCGACATAGGCGATCCTGCGGTGGCCCAGCGCGTGCAGATGGGTCAGCGCGTCGGTGATCCCCTCCACCAGGTCGGCCGTGACGCATCCGAACCCCGGCTCCTTTCGGTGCAGCAGCACGATCGGCTTGTCGGTGAGCTCGCCCAGCGACCCCAACTCCTCGCCGCTCAGATGCGGGGAACACAGCACGATCCCGTCCACCTGCGGGGCCAGGGTGCGGATCGCGTCCAGCTCCAGGCCCTCGTCCCGCTCGGCGTCGCTCAAGAACACCGTGTGGCCGAGCCCCCGGGCCCGCGCCTGCACCCCCTTGCAGATGTCCGCGAAGAAGGGGTTCAGCAGATCCGGGAC GATCACGCCCAGGTGGCCCGTGCGCCCGGTGATCAGCCCGCGCGCGGCCTGGTTGGGCTGATAGCCGAGCCGGTCGGCGGCGGCCCGGACCCGCTCGCGCGTCACGGGGCTCACCGTCCCGCCGGGCGTCAGGGCCCGCGACACGGTGGACGGGGACACCCCCGACGCCTTCGCGACGTCCCGAATCGTCACCTGCACGCCCCGAGGTTGACACCCCGTTCCACCCGCGTCAAACATCGTCCCCCCAGGCCACCACCGGCTTCACGGTGTTAGGAACTCTTAAGACGCCCTTGTGAGACTTGCGCGGACCGGTTTTCGTACCTGTCCACGCTTGAATCGAGGCACCGTGTCTCCTTCGGAAGCCACGTCCACTTCGTCCACCGCAGCCGCGTCTCCCGACTTCCTCGATCCGTCCTTCTGGGCCCGGCCGCTCGATGAGCGTGCGGCGGTGTTCGAGAGGCTGCGTGGGCTGCCGCACCCCGAATTCGTGTCGCCGCGGCTGCCCTGGGGGCCGATGGCATCTGGCTATTACGCGCTGGCGAAACACGCGGACATCGCCGAGGTGAGCCGGCGCCCTCAGGACTTTTCGTCCGAGGGCGCCACCACGATGCTGACGCCGGAGATGGAAGAGTTCTACGGCTCCATGATCACCATGGACAATCCGGAGCACGCCAGGTTACGGCGCATCGTGGCGCGGTCGTTCGGCCGCAACATGGCGCCCAGGTTCGATGCCATTGCCCGTCAGGTGGCCCGGCGGATCGTGGACGAGCTCATCGAGCGCGGGCCCGGCGACTTCGTCCGCCCGGCGGCGGAGATGCCCATCGCCGTGCTCAGCACCATGATGGGCATTCCCAGTGAGGACTACGAATTCCTCTTCGAGCGGACGAACATCATCATGGGCGGGCTCGACCCCGAGGTCGCGGCCAGCCCCGAGGACGCGGCGGCCGCCGTGCTGGGCGCCCTGCGGGACCTCGGGGACTACATCGGACGGCTCCGCGAGGACCGGCTCGCCCGCCCCAGCAACGATGTCATCACCAAGCTGGTCCAGGTACAGGACGACGGAGAACAACTCACCCGCCAGGAACTGGTCTCCTTCTTCATCCTCCTCGTCAACGCCGGCATGGAGACCACCCGCAACTCCATCGCCCAGGCCCTGGTGCTGCTCACCGCCCACCCGGACCAGCGGCAACTTCTGCTGTCGGACTTCGAACGCCATGCGAAGGGCGCCGTCGAGGAGATCCTCCGGGTGGGCACCCCCATCAACTGGATGCGCCGGACCGCCACCGGCGACATCGAGATGAACGGCCATCGGTTCCGCAAGGGCGACCAGGTTTTCCTCCTCTACTGGTCCGCGAACCACGACGGACACGTCTTCGAGGATCCGTACCGCTTCGACATCACCCGGGACCCCAATCCGCATCTGTCATTCGGCGCGGTGGGGCCGCACTTCTGTCTCGGCGCCCATCTCGCCCGGATCGAGATCATCGCCATGTACCGAGAACTCCTGGCCTCACTCCCGGACATCCGCTCGGAGGGCGAGCCCATCCGCCTGGATTCCAGCTTCGTCGAGGGCTTCAAAGGTCTGAACTGCACGTTCTGACGCTAAAAGAGAGGAGGCGCCAGCGATGGCGAAGGACGAGGCGAAGCTTCTCGACCACCTCAAGTGGGTGACGGCAGAACTGCGTGACACTCGGCAGCGTCTGCGCGAAGCGGAGTCCGCCGAACCCGAGCCGATCGCGATCGTCGGAATGGCCTGCCGGTTTCCCGGCGGGGTTGAGAACCCGGACGACTTGTGGAATCTGGTGGCGGCCGGAGGCGAAGGGCTGGTCGATTTCCCGGAGGACCGCGGCTGGGATCTGGAGAACCTGTTCGATCCCGACCCGGACCGGCCGGGGACGAGTTATGCGCGCCAGGGCACCTTTCTGCGCGGCGCCGGAGGCTTTGACGCCGAATTCTTCGGGATATCGCCGCGTGAGGCGGTGGTGATGGACCCCCAGCAGCGCGTGTTGCTGGAGGTCGCCTGGGAGACCTTCGAACACGCCGGCATCGACCCGCACGGCCTCGCGGGCAGCAGCACGGGCGTCTACGCCGGGGTGACCTCACAGGACTACATGGTGCTCACCGCCATGGCCGCCAATGACGTGGAGGGATACGCCGCCACCGGAAACCTCGCCTGTGTCCTGTCCGGGCGGGTGTCCTATGAGTTGGGGCTCGAGGGTCCTGCGGTGACGGTGGACACGGGGTGTTCGTCGTCATTGGTGGCGCTGCATTCCGCCGTGCAGGCGCTGCGCGGTGGCGAGTGTTCGCTGGCGTTGGCGGGTGGCGTGACGGTGATGGCGACGCCGGGTGCGTTTGTGGAGTTTTCGCGGCAGCGGGGGTTGGCGGCGGACGGGCGGTGCAAGTCGTTCGCGGCGGCGGCGGATGGTACGGGGTGGGGCGAGGGTGTCGGGCTGTTGCTGCTGGAGCGGCTGTCGGACGCGCGGCGGAACGGCCACAAGGTGTGGGGGGTGGTGCGGGGTTCGGCGATCAACCAGGACGGTGCCAGCAATGGGCTGACGGCGCCGAACGGTCCGTCGCAGCAGCGGGTGATCCGGCAGGCGCTCGCGAACGCGCGGCTGTCGCCCGCCGATGTGGACGCGGTCGAGGCGCATGGGACGGGCACCACGCTTGGCGATCCGATCGAGGCTCAGGCGTTGCTGGCCACCTACGGTCAGGGGCGGGCGGCCGAGCGGCCGTTGCTGCTGGGGTCGGTCAAGTCCAACATCGGTCACACCCAGGCGGCTGCCGGAGTGGCGGGCATCATCAAGATGGTCATGGCGATGCGGCACGGGCTGTTGCCCAAGACGCTGCACGTGGACGAGCCGACGCCGCATGTGGACTGGAGCGGCGGAGCGGTCGAACTGCTCAGGGAGGCGACGGCCTGGCCGGACGCGGAGCGCCCCCGGCGGGCGGCTGTCTCGTCGTTCGGCATCTCCGGCACCAACGCCCATGTGATTCTGGAAGAGGCCACAGCACCGGCGGCGGACGTGGCGACCACCGAGCCGACGGTCCCCGGTGGCGTGGTGCCGTGGGTGGTCTCGGGGCGGTCGGCGGCGGCGCTGCGGGCCCAGGCGGAGCGGTTGGCGGAGTTCGCCGAGCAGACCTTCGCCGACCCGCCCGAGGTGGGCTGGTCGCTGGCGGCGGGCCGGGCGATGCTCGACCACCGGGCGGTGGTCGTCAGCGACGACCGGGAGGAGTTGCTGGCCGGTCTGAGGGCGCTGGCCGTGGGGGCGCCGTTCGGTGGTGTGGTGACCGCGGAGCCGGTGGCCGGGGGTGCCGGGCCGGTGCTGGTGTTCCCGGGCCAGGGCGGTCAGTGGCGGGGGATGGGGGTGGAGTTGCTGGACTTCTCCCCGGTGTTCGCGGCGCGGATCGCGGAGTGCGAGGCGGCGCTGTCGCCGTTTGTGGACTGGTCGCTGACGGCGGTGCTGCGGGGCGAGGGCGGGGTGGACGCGTCGCGGGTGGATGTGGTGCAGCCGGCGTTGTGG GCGGTGATGGTCTCGCTGGCGGAGGTGTGGCGTTCCCATGGCGTGGCGCCCGCGGCGGTGGTGGGGCATTCGCAGGGCGAGATCGCGGCGGCCGTGGTGGCCGGGGCGCTGTCGCTGGAGGACGGGGCGCGGGTTGTCGCTGTGCGCTCGCAGGCGTTGGGGGTGTTGTCGGGCCGGGGTGCGATGGCCTCGCTCGCGGTGGGTGCCGGGGAGGCCGAGGAGGCGATCGGCGGGCGTGAGGGTGTGGTCGTGGCGGCGGTGAACGGGCCGGGGTCGACCGTGATCTCGGGTCCGCCCGGGGCGGTGGCCGAGGTGGTGGCGGCGGTGGAGTCGGCCGGTGGGCGGGCGCGGCTCATCGACGTCGATTACGCCTCGCACGGCCCGCAGGTCGACGAGATCGCCGAGGATCTCGTGGCGCTGCTGGGGGAGATCACTCCGGTGGAAACGGCCACGGCGTTCTACTCGACGGTGACCGGTGGCCGGGTGGAGACCACGGCGCTGGACGCGGCGTACTGGGTGGAGAACCTGCGCCGTCAGGTCCGGTTCGGCACCGCGGTGGAGGCGGTCCTGGGCGAGGGGTACCGGGTGCTGGTGGAGGCCGGCCCGCATCCGGTACTGAGCGTGGGCATCCAGGAGACCGCCCAGGCATGCGATATCGCGGTGGCCACGGTGCCCACCCTCCAGCGTGACCAGGGCGGCCCGGCGCAGCTGGCCCGGGCCCTGGCCCAGGCCTTCGCCGCCGGGCTGCCCGTGGACTGGAAGACCTGGTACGGCGTGACCGCAGCCGCGCCACCCGTCCTCGACCTGCCCACCTACCGCTTCCAACACCAGCACTACTGGCTGAAGCCGGGTACCTGGGCGGGGGCCGAGGGAAAGGGCTCCGCAGAGGACGAGCGCTTCTGGAGCGCCGTCGAGAGCGGCGATCTCGCCGGGCTCGAGGCATCGCTGCGCGTGGGGGAGGACGCCGCGCGACGCGTGCTGGCTCCGGCGCTGCCGGTGCTGGCGGAGTGGCGTCGGCGTACCCGGGATCAGGCCCGGATCGACGCATGGCGCTACCGGACCGCCTGGTCCGCCGTCACCGGCCCGGACGCGGCTCCCCGGCTGAGCGGCACATGGTTGCTGGTCGTCCCCGAGGAGTGGGCCGGGGACGCCGCGGTCGAGACGGTCACCCAGGCGCTCGAAGGCCATGGGGCGGAGTGCGTGCTCCTCCCGCTGGCCCCGGGGGACCAGGCGCGCGGCCCCCTCGCCGAGCGGCTGCGGCAGTTGGAGGGCCGGCCCGAGATCGTCGGTGTGATCAGCGCCTTGAGCCTCGACGAGGCCGTACACCCGGAGCACCCGCATCTGCCGATGGGACTGGCCGGGACGCTCGCCCTGGTGCAGGCGCTGGACGAGGTGGACTTCGGCGGGCGCCTGTGGTGTGTGACGCGCGGCGGTGTGTCCGCCGCCGGGGACGGCGCCGTAAGCCCGGTGCAGGCCCAGGTGTGGGGTCTGGGGCGCGTGGTGGCGCTGGAGTATCCGAAGCGGTGGGGTGGCCTGATCGATCTTCCGGCGGTGGCGGAGGAGGGCACGGCGGGCCGGCTGGCCGCGGTGGTGGCCGAGGGCGCGGAGGACCAGGTCGCGCTGCGTGCGGAGGGCGTGCTGGGGCGTCGCCTGCGTGCGGCGTCGGCGGGTGCGCCGGGTGCGGAGTGGCGCATCGACGGCTCCGTTGTGGTGACCGGCGGGACGGGCGGGGTGGGCGGCCGGGTCGCCCGGTGGGTCGTGGAGCGGGGCGCGCGCCATGTGATCCTGGCAGGCCGCCGGGGCTCCGCCGCGCCGGGGACCGAGGCGCTGACGGCGGAGCTGGAGGCGCTGGGCGCCTCGGTGGACGTGGTGGCCTGCGATGTCGCGGACCGCGACGAGGTGGCCGCGCTGCTGGCGCGGGCGCCGGAAGAGCATCCGCTCCGTGGGGTCTTCCACGCCGCGGGGGTCGGCGACTACACCCCGGTCAGCGACCTGGACCCGGACCGGGTGGCGCAGGTGGCGGCGGCGAAGGCGGGGGGTGCGCGATGGCTCGATGAGCTGACGCGCGACATGGACGTGTCGGCCTTTGTGCTGTTCTCCTCCGGCGCGGCCAGTTGGGGTAGCGGGCAGCAGGGTGCGTACGCGGCGGCCAACGCGTACCTGGACGCGCTGGCCGAACGCCGCCGCGCCGAGGGCCTGCCGGGTCTGTCGGTGGCATGGGGCCCGTGGGGCGAGGTCGGCATGACCGCCGGCGACGCGGTGGCGGCCTTCTTCCGCGAGCGGGGGCTGACCGCCATGGCTCCCGAGTTGGCGCTGCGGGTACTGGGCGATGCCCTGGGCCGCGGCGAGACGGCGTTGACGGTGGCCGACTTCGACTGGCGGCGCTTCGCCGCGGCGTTCGCCGGGCAGCGCGTCAGCAGGCTGCTCGCCGAGATTCCCCAGGCCGTCGAACTGCTGGAGAAGGAGACGCCGTCCGAGGACAGCCCGCTGCGCAAGCAACTGGCCGCGGCCGTCCCGGAGCAGCGGCACCAGATGCTCACGCAGCACATCCGCGCCCTGGCGGCCGGGGTGCTGGGCCACTCGGGCCCGGACGCGATCTCCGCGACCAAGCCGTTCTTCGAGATGGGCTTTGACTCGCTGACCGCTGTCGAGCTGCGCAACCGGCTCAGCGCCAGCGCCGGGATGCCGCTGCCCACCACACTGATCTTCGACTACCCCACCGCGGACGACCTGGCCCGTTACGTCCTCGCGGAAATCTCCGGCCTCCAGACGGCCGTGACCGGCACGGTCACGCCGGTCGGGGAATCGGACGAGCCGATCGCGATCGTCGGTATGGCCTGCCGGTTCCCGGGCGGGGTGAGCAGCCCCGAACAGCTCTGGGACCTCGTGGCCACGGGCCGGGACGCGATGTCGCCCTTCCCGGCCGACCGAGGCTGGCGTCTCGACACGCTGTACGACCTGGACCCGGACCGCCCCGGCACCAGCTATGTGCGCGAGGGGGGATTCCTCCACGACGCGGGGGGTTTCGACGCGGAGTTCTTCGGGATCTCGCCGCGTGAGGCGGTGGGGATGGACCCTCAGCAGCGGCTGTTGCTGGAGACCGCCTGGGAGACGTTCGAGCGCGCCGGGATCGACCACGGCTCGCTGCGCGGCAGCGACACCGGTGTCTATGTCGGCGCGACCCTCTTCGACTATCTGTCGATCATCGGGATCTCCAGCATGGACATGGAGGGCTACACCGGCACGGGCAACCTCGGCTGTGTGCTGTCGGGGCGGGTGTCCTATGTGCTGGGGCTCGAGGGGCCGGCGGTGACGGTGGACACGGGGTGTTCGTCGTCGTTGGTGGCGCTGCACTCGGCGGTGCAGGGGTTGCGTGGCGGTGAGTGTTCGCTGGCGTTGGCGGGCGGTGTGACGGTGATGGCGACGCCGGGTGCGTTTGTGGAGTTTTCGCGGCAGCGGGGGTTGGCGGCGGACGGGCGGTGCAAGTCGTTCGCGGCGGCGGCGGATGGTACGG GGTGGGGCGAGGGTGTCGGGCTGTTGCTGCTGGAGCGGCTGTCGGACGCGCGGCGGAACGGCCACAAGGTGTGGGGGGTGGTGCGGGGTTCGGCGATCAACCAGGACGGTGCGAGTAACGGCCTGACGGCGCCGAACGGCCCGTCGCAGCAGCGGGTGATCCGGCAGGCGCTCGCGAACGCGGGGTTGTCGACGGCGGACGTGGATGTCGTCGAGGCGCACGGGACGGGCACCACGCTTGGCGATCCGATCGAGGCTCAGGCGTTGCTGGCCACCTACGGTCAGGGGCGGTCGGCCGAGCGGCCGTTGCTGCTGGGGTCGGTCAAGTCCAACATCGGTCACACCCAGGCGGCTGCCGGAGTGGCGGGCATCATCAAGATGGTCATGGCGATGCGGCACGGGGTGGTGCCCAAGACGCTGCACGTGGACGAGGCGACACCGCATGTGGACTGGGGCGGCGGGGCGGTCGAACTGCTCAGGGAGGCGACGGCCTGGCCGGAGGTGGAGCGCCCCCGGCAGGCGGGCGTGTCGGCGTTCGGTATCTCCGGGACGAACGCGCACGTGATCGTGGAAGAGGCTCCCAAGGAGCCGGAGGCTCCGACCGAGTCCGGCGCGGTGCCGGTGGGTGGTGTGGTGCCGTGGGTGGTCTCGGGACGGTCGGCGGCGGGGCTGCGGGCCCAGGCCGCGCGGCTGGCGGAGTTCGCCGAGCGGACCGCCGCCGATGAGGCGGAGGTCGGCTGGTCGCTGGTGGCCGGCCGAGCCACGCTGGAACACCGCGCGGTGGTCGTCGGCCAGGACCGGAAGGAGCTGCTGGCCGGGTTGACGGCGCTGGCCGAGGGCGAGCCGTCCGGTGGTGTGGTGACCGCCGAGCCGGTGGCCGGGGGTGCCGGGCCGGTGCTGGTGTTCCCGGGCCAGGGCGGTCAGTGGCGGGGGATGGGCGTGCAACTGCTCGACGCCTCGCCGGTGTTCGCGGCGCGGATCGCCGAGTGCGAGGCCGCGCTGTCGCCCTTCGTGGACTGGTCGCTCACGGCGGTGCTGCGGGGCGTCGACGAGACGGCGGACATCGACCGGGTGGATGTGGTGCAGCCGGCGTTGTGGGCGGTGATGGTCTCGCTGGCGGAGGTGTGGCGCTCCCATGGCGTGGCGCCCGCGGCGGTGGTGGGGCATTCGCAGGGCGAGATCGCGGCGGCCGTGGTGGCCGGGGCGCTGTCGCTGGAGGACGGGGCGCGGGTTGTCGCTGTGCGCTCGCAGGCGTTGGGGGTGTTGTCGGGCCGGGGTGCGATGGCCTCGCTCGCGGTGGGTGCCGGGGAGGCCGAGGAGGCGATCGGCGGGCGTGAGGGTGTGGTCGTGGCGGCGGTGAACGGGCCGGGGTCGACCGTGATCTCGGGTCCGCCCGGGGCGGTGGCCGAGGTGGTGGCGGCGGTGGAGTCGGCCGGTGGGCGGGCGCGGCTGGTCGATGTGGACTACGCCTCGCACGGCCCGCAGGTCGACGAGATCGCCGAGGAGCTGGTGGCGTCGCTGGGAGACGTCAAGCCCGCCGGCACCGGGGTGGCGTTCTATTCGACGGTGACCGGTGGCCGGGTGGAGAGCACGGTGCTGGACGCGGCGTACTGGGTGGAGAACCTGCGCCGTCAGGTCCGGTTCGGCACCGCGGTGGAGGCGGTGCTGGCCGACGGATACCGGGTGCTGGTGGAGGCCGGTCCGCATCCGGTGCTGAGCGTGGGCATCCAGGAGACCGCCCAGACGCTGGACCTTCCCGTGGCCACGGTGCCCACTCTCCAGCGTGACCAGGGTGGCCCGGCGCAGCTGGCCCGGGCCCTGGCCCAGGCCTTCGCCGCCGGGCTGCCCGTGGACTGGAAGACCTGGTACGGCGTGACCGCAGCCGCGCCACCCGTCCTCGACCTGCCCACCTACCCCTTCCGACACCAGCACTACTGGATCGACCCCGCCACCATCGGCGGCCAGGGCGATCCCCACAGCCTCGGCCTGACCTCCGCCGACCACCCGCTGCTCGGCGCCGCCGTGCGGGTCGCCGAGAGCGAGGAGATCGTGCTGACGGGCCGGGTCTCCACCGCCGGACACCCCTGGGTGGCCGACCACGCCGTGGCCGGTTCGGTGTTCCTGCCCGGCACGGCGTTCGTCGACCTCGCCGTGCGCGCGGGGGACGAGGCAGGCTGTGACCGCGTCGAGGAACTGGTCCTGGAAACCCCCCTGATCCTCCCCGAAGAGGGGGCGATCCAGCTCCAGCTCGTCGTCTCCGCGCCAGGCCAGGACGGCGGTCGAGCCTTCACCGTCCACGCGCGCGCCGAGGGCGACGACGACGCCGACCGGCCCTGGACCCGGCACGCGAGCGGCACCCTCACCACAGGCGCCCGGCCGGACGACTTCGACTTCGCCCAGTGGCCGCCGACCGGTGCCGAGCCGGTGCCGCTGGACGGTCTCTACGCGGGCCTGGCCGAGGCGGGCTACGGCTACGGCCCGGCGTTCCGCGGCCTGAAGGCGGCCTGGCGCCGCGGTGACGAGGTGTTCGCCGAGGCCGCGCTCGCCGAGGAACTGCGCGGCGACGCGGGGCGGTTCGGTCTGCACCCCGCCCTGCTGGACGCAGCGCTCCAGGCCGGCGGCATCGGGAAGGGCGGTGCGCTCGACGGGCAGCTGCTGCTGCCGTTCACCTGGACCGGAGTGTCGCTGTACGCCACCGGCGCCGCCGCGCTGCGGGTCCGGATGGTTCCAGGCGGCGACGGGGTGGCCCTGAGCGTCGCGGACCCCTCGGGCCGCCCGGTCGCGTCCGCCGAGGCGGTGGTCTCCCGCCCGGTCTCCATCCGGCAGTTGCGCCGCGGCGGTGCCGAGCTCGACTCGCTGTACCGCGTCGAGTGGGTCTCCACCGAGTACGAGCCCGAGCCCGGGGTGTTCGCCGGCCGTACGTGGGACGTGGTGGGTGCCGGCGGCCTCGGCATCGAGGACGCACTGGAACGCGCAGGACACACCGTCCGCACCACCCGGGACCTCGCGGAGCTGGCGCGGTCCGTCGACGACGGGACACCCCTGGCCGACCTGGTGGTACTGGACTGCGCCCCGCTCGCCGGTGGCGGAACCGAAGCCGACCCCGGACTCGCCGACGCGGTCCACGACGAGACCGAACGGCTGCTCGGCGTCGTCCAACGCTGGCTCGGGGACGAGCGGTTCGGCGACTCCCGGCTGGCGCTGGTGACCCGCGGCGCCCAGTCGACCAGCGGCGACGAGCGCGTCGCCGACCTGGTGCACGCACCGCTGTGGGGCCTGGTGCGCTCGGCGCAGATCGAGAGCTACGACCGGCTCGTGCTGGTGGACCTGGAGGACGGCGACCAGGACGCGGCCGGCCTGCTGCCCCGCGCCCTCGCCGTGGCGCTCGGGGCGGGCGAGCCGCAGGTGGCGGTGCGCCGCGGCCGTCTGGTCGTCCCGCGCCTGGCCCGTATCGGCAAGGACACCGCCCTCACCCCGCCGGACGTGCCCGGCTGGCGCCTCGACACAGC CCGTCCCGGCACTCCCGGCACTCCCGGCACTCCCATCGCTCCGGACCAGTTGGCCCTGGTGCCTCACCCCGAGGCGGCCGGGCCGCTGGGGGAGGGCCAGGTCCGCATCGCCGTACGGGCCGCGGGCCTCAACCCCCACGACGTCGTCGCGGGCCCGGACCCGCTTCCCGACCAGCAGCCCATGGGCGGTGAGGGGGCCGGGGTCGTCGTCGAGACGGGGCCCGGTGTCATCGGACTCGCCCCCGGCGACCGTGTGATGGGCGTCTTCCCTCGCGCGTTCGGCCCGCTGGCCGTCGCCGACCAGGGGAGCCTCGTACCAGTCCCCGCCGGCTGGTCCTACGCCCGGGCCGCCGCCGTGCCCCTGGCGTTCCTCACCGCCTACTGCTGCCTCGCCGAGCCCGCCGAGGTCCGTGAGGGCGACGTGGTGCTCGTCCACGGGGCCGCCGGTGGCGTCGGCATGGCCGCCGTGCAACTGGCCCGGCATCTCGGCGCCGAGGTCTATGCCACCGCCGATGAGAGCGAGTGGGACGCCCTGCGCGCCCTCGGCCTGGACGACGACCACCTGGCCTCCTCCCGGACCCTCGACTTCGAGGACCGGGTGCGCGCCGCCACCGGCGGCCGGGGCGTGGACGTGGTGCTGAACTCCCTCGCCCAGGAGTTCACGGACGCCTCGCTGCGCCTGCTGCGCGACGGCGGCCGGTTCGTGGAGCCGGGCCGGACCGACGTCCGCGATGGCGCGCAGCTCCATGAACCAGCCACCGCCCACCCGGGCATCGGCTATCGGGTGTTCACGGGGGCCGACCCGCGGCGGATCCGCGCGATCCTCACCGAGGTCGTGGGCCTCATGGAGCGGGGCGTGCTGCGTCATCTGCCCCTACGGACCTGGGATCTGCGCCTCGCCCCCGACGCGTTCCGGTATCTGAGCCGGGCCCGGCACACCGGCAAGGCCGTGCTGACACTCCCGGCGCCCCTCGACCCCGAGGGCACCGTGCTGATCGTCGGCGGCACCGGCACGATCGGCGGCATGCTCGCCCGCCACCTGGTCACCACCCACGGCGTTCGCCACCTCCTGGTGACCGGCCGGCGGGGTGCCGAGGCGCCCGGCGCGGACGCGCTGCGCGAGGAACTGTCCGCTCTGGGGGCGGAGGTGACCATCGCCGCCTGCGATGCCGCCGACCGCGACGCCCTGGCGGCGCTGCTCGCGGACATCCCCGCCGAGCACCCGCTGACCGGCGTGATCCACGCCGCCGGTGTGGTGGACGACGGCACCGTCCCCTCCCTCACGCCCGAGCAACTGCACACCGTGCTGCGGCCCAAGGTGGACGCGGCGGTCAACCTGCACCAGCTGATCCGTGAAGACGATCTCGCGGCGTTCGTGATGTTCTCCTCGACGGGCGGCCCGGGCGGCGCCGCGGGCCAGGGCAACTACGCGGCGTCGAACGCCTTCCTCGACGCCCTCGCCCAGGACCGCCGGGCCGCCGGGCTGCCCGGTCAGTCGCTGGCCTGGGGCCACTGGGAGCAGAGCAGCGGCATCACCGGCACCCTGGGCGAGCGCGACATCGCCCGCATGGAGCGCTCCGGTGTGCGTGCGCTCTCCTCCGACCAGGGTCTCGCCCTGTTCGACGCGGCCGGGCGCCGGCCCGAGAGCCTGCTGATGCCCGCCCGCCTCGACCCGGAGGTGCTGCGGGACCTCGCCGGCGCCCAGGTCCTGCCCCGCGTCCTCAGCGGCCTGGTGCGGGCGGCACCCGCCCGGCGCGCCACCGCGGCGGCGCAGTACGCGGCCGGGGACGCCCAGCCGATGGCGGAGCGCCTGGCGGGCATGTCCGCCGCCGAGCAGGGCCGCACCCTGCTGGAACTGGTGCGCCGCAACGTGGCGGCGGTGCTCGGGCTCGGCGACGTCCTCGCCGTCGACCCGGCGCGGCCCTTCAAGGAGATCGGCTTCGATTCGCTGACCGCGGTCGAGCTGCGCAACCGGCTCAGCCAGGCCACCGACACCAAGCTGCCCTCCACTCTCGTCTTCGACATCCCCACCCCGGCGCTGCTCGCCGAGCACCTGCGCGAACAGCTCGTCTCCGAGGGGCTGAGCGGGGCGGAGGCCCTCGTTCAGGAACTGGACCGGCTGGAGGAACACGTCGACCTGCTCGCGGACGACACGGAACGGGACACCGTGACAGCCCGGCTCGAGGCGCTGCTGACACGGTGCCGCCAGAAACCCGCGGCGGAGAGCAACGGCGTCGCGGAGCGGCTCCAGGACGCCTCGGCCGACGAAGTCCTGCAATTCATCGACTCCCACCTGGGAAGGGCCTGAAAGAGGACCGCCATGGCGAACCAGGAACAGCTCGTCGACTACCTCAAACGAGTGGCGACCGATCTGCATGACGCTCAGCAGCGCCTCCGCGAGGTGGAGGCCCGGGACCGCCAGCCGATCGCGATCGTCGGCATGGCCTGCCGGTTCCCTGGTGGAGCGGACACTCCGGAGGCCCTGTGGGAGCTGGTGTCCGCGGGCCGTGACGCGGTCTCCGCCATGCCGGATGACCGGGGCTGGGACCCCGGCATGTTCAACGACTCCGGCGAGACGGGCACCAGCTATGTGCGCGAGGGCGGATTCGTCCGCGGCATGGCCGACTTCGACGCCGACTTCTTCGACATCTCGCCGCGCGAGGCGCTCGCGATGGACCCGCAGCAGCGCCTGCTGCTGGAGACCGCCTGGGAAGCCATCGAGCGCGCCCGCATCGACGTGACCTCCCTGCGCGCCACCAGGACCGGCGTCTTCATCGGCGGATCCACCATCTACTACGCCGGCACCGGTGCCGGCGCGCCGCAGGACGTCGCCGGGTACATGGCCACCGGGCTGGCCGCGAGCTCGATGTCGGGCCGCATCTCCTACACCTTCGGCTTCGAGGGCCCCTCCTTCACGGTGGACACCGCCTGCTCGTCGTCCGGGGTGGCCCTGCATCTGGCGGTGCAGTCGCTGCGCAAGGGTGAGAGCTCGCTGGCGCTGGCCGGAGGGGTGTGCGTGATGGCGACACCCGGCACCTTCCTGGAGTTCAGCAAGTTGAACGGACTGGCCGCCGACGGCCGGTGCAAGGCGTTCGCGGCGTCGGCCGACGGCTTCGGGCCCGGCGAGGGCGTGGGCGTCCTGCTGGTGGAGCGGCTGGCGGACGCCCAGCGCCTCGGACACCCGGTGCTCGCCGTGATCCGCGGCTCCGCGATCAACCAGGACGGCGCATCCAACGGCCTGACCGCGCCCCACGGCCCCGCCCAGGAACGCGTCATCCGCGCCGCGCTGGCCGACGCCCAGCTGTCCGCCCGGGACATCGACGTGGTGGAGGCGCACGGCACCGGCACCTCGCTGGGCGACCCCATCGAGGCACAGGCGCTGATCGCCGCGTACGGCAGAAGGCGCGGCGAAGGCGCTCCGCTGTGGCTCGGCTCGGTCAAGTCCAACATCGGGCACACCCAGGCCGCCGCCGGCATGGCCGGGGTGATCAAGATGGTGCAGGCGCTGCGGCACGGCGTCCTGCCGA GCACCCTGCACGTGGACGAACCCACCCATCAGGTGGACTGGTCCGAGGGCACCGTGAAGGTGCTGACCGAGGCGCGGCCGTGGCCCGGGACCGGCGGCCCGCGCCGGGCCGGGGTCTCCTCCTTCGGCATGAGCGGCACCAACGCGCATGTCATCCTCGAAGAAGCCCCCGAGGCCTCCAACGCGGCGGAGCCCGCCCCCGCCACCGAGCTGCCGGTGGTCCCGTGGCTGGTGTCCGGGCGCGGCGAGGCGGCGCTGCGCGCCCAGGCCGCCCGGCTGCGCGACTTCCTCACCGAGCGGCCGGAAGCCGGCCCGACCCGCGTCGGGCTCGCCCTGGCCGGTTCGCGCGCCGCCCAGTCGCACCGGGCCGCCGTGGTCGCCGCCGACCGCGACGCCCTGCTGGCCGGGCTGGCCTCCCTGGCCGACGGGGTCCCCGCCGGCCATGTGGTCACGGGTGCGCCCAGCCCCGGCGAGGCGGTGTTCGTCTTCCCCGGCCAGGGCTCGCAGTGGGCCGGCATGGCCCTCGACCTGGCCGACGCCTCCCCGGTGTTCGCCGAACACTTCCGCCGCTGCGCGCAGGCCGTGGAGCGGCACACCGAGTACACCGTGGAGTCGGTGCTCAGAGGCGAGCCGGGAGCCCCCTCCCTGGAGCGGGTGGACGTGGTCCAGCCCGTGCTGTGGGTGGTGATGGTGGCGCTGGCCGAGCTGTGGCGGTCCTACGGCGTGGAGCCCGCGGCCGTGGTGGGCCACTCCCAGGGCGAGATCGCGGCGGCCTGCGTCGCCGGCGTGCTGTCCGTCGAGGACGCGGCCCGCGTCGTGGCGCTGCGCTCCCAGGCGCTGCCCGAGCTGTCCGGCCGCGGCGGCATGGCCTCCGTGGCGCTCCCCGTCCAGCAGGTCGGGAAACACCTGGAGGGCTGGGACGGCCGGCTGGGCGTGGCCGCCGTCAACGGCCCCTCCTCGACCGTGGTCTCGGGAGACGCCGACGCACTGGAGGAGCTGCTGGAGACCTGTGAGGCCGACGGGGTGCGCGCCCGGCGCGTCCCCGTGGACTACGCCTCGCACTGCGCCCACGTCGACGCGCTGCGCGACCGGCTGGCCGAGGACCTCGCGGGCATCGCGCCCAAGCCGGGCTCCGTCCCGCTGTACTCGACCGTCACCGGGCAGCGCATCGACGGCACCGCCATGGACGCCGGGTACTGGTTCACCAACCTCCGTCAGCAGGTGAGGTTCGAAGAGGCCACCCGGGCGCTCCTCGACGACGGACACAGCGTGTTCATCGAGTGCAGCCCGCACCCGGTCCTCGTCATGGGCGCGCAGGAGACCGTGGACGAGGCGGGCGTCAACGCCACGGCCCTCGGCACCCTGCGCCGGGACGAGGGCGGCTGGGACCGCTTCCTCCTCGCGCTGGCCCAGGCCCATGTCCATGGCGTCGAGGTGGCCTGGGGCCGGGTGTTCCCGGACGGCACCGGCCCCGCCGACCTGCCCACCTACGCGTTCCAGCGGCGGCGCTACTGGCTGGACGCCACCGACGGCGGAGCCGGTGACCCCGACCGGCTCGGCCTCGCCGCGGCCGGCCATCCGCTGCTGGGGGCCGTCACCGTCCTCGCCGAAGGGGACGAGGTGGTGCTGACCGGCCGGCTCGGCCTGGACACCCATCCGTGGCTGGCCGACCACGCGGTGGCCGGCGCGGTGCTCGTGCCCGGCGCCCTGTTCGTGGAGCTGGCCGTCAGGGCCGGTGACGAGGTGGGCTGCGACTGGCTCGAGGAACTGGTGCTGGCGAGCCCGCTGGTCCTGCCCGACCAGGGCGGCACCCACCTTCAGCTCGTGGTGGGCCGCCCCGACGGTGAAGGCCGCCGCACCCTGAACGTGTACGCACGCCCGGCCGACGAGTCCGGCTCGCCATGGCTGCGCCACGCCACCGGCACCCTGGCGCCCGGCCCGGACGCCGAGCCGTACGACCTCGCCCAGTGGCCGCCGGAAGGCACCGAGCCCGTGCCCGTGGACGGCTGTTACGACCAGCTGGCCGAGGCCGGCTACCGCTACGGCCCCGCCTTCCGCTGCCTGCGCGCCGTGTACCGCCGCGGGACGGAGGTCTTCGCCGAGGTCGTCCTCCCGGAGGAGCAGCGCGGGCAGGCCGCCGCGTTCGGTGTCCACCCCGCGCTGCTGGACGGTGCGCTCCAGGCCAGTGGGTTGCCCGAAGTACGCGGTGGCCAGGGCCGGATGGCGCTCCCGTTCGCCTGGAACGGAGTGTCCCTCGCCGCCACCGGCGCCGAGCTGCTGCGCGTCAGGCTCGCGCCGGTCGGCGACGACGGCATGTCCGTCCACGCCACCGACGCCTCGGGCCGCCCGGTGATCTCGATCGACGCGCTGGTGACCCGGCCGTTCACCCCCGAGCAACTCCAGGGCGGCGGCGAGGACATCGGCGACGCGCTCTTCCGCGTGACGTACACCCCCCTGACCGGTGCCCCGGCGGGCGGCGCCATCGACGGCTGGGCCGCCGTCGCCACCGGCACCACACCCACCTGCTACGGAGCGCCCCGCTACGACGATCTGGACGCGCTCGCCGCCGCGGTGGACGGCGGCCTGCGCGTCCCGCCGGTGACACTGCTGCCCTGCGAGCCGCGGCCGGACGACACCGACCTCCCCGGCGCGGTGCGCCACCGGCTGGGCGAGGTGCTGCACTCCGTGCAGCGGTGGCTGGCCGACGAGCGGTTCGCCGACTCCCGGCTGGTGGTGGTCACCCGGGGCGCGGTCGCCGCCTCCGACGGCGAGGACGTCACCGACCTCGCGCACGCCCCGGTGTGGGGCCTGATCAGGTCCGCGCAGTCCGAACACCCGGACCGGCTGGTCCTGGTGGACCTGGACGGACCCGCGCTGCCCGGGGCCCTTGCCCCGGCGGTCGCCGCGGGCGAGCAGCGGATCGTCGTCCGGAACGGGACGGTACGGGTCTCCGCCCTGGTCCCCGCGGCGTCCGGCGCCGCCCTGACCCTGCCGGAGGACCCCCACTGGCGGCTGGACATCACCGGCCCCGCCACCCTGGACAACCTGGCCCTGGTGGCGGCGGAGGAGCCCCCGCCGCTCGCCCCGGGCCGGATCCGGGTCCGGGTCCGCGCCGCGGGCGTCAACTTCCGGGACGTGCTGATCGCGCTCGGGATGTACCCGGGCGACGCGGTGATCCGGGGCAGCGAGGGCGCGGGCGTGGTGGTCGAGGTGGCCCCGGACGTGACGTCGGTGGCCGTCGGCGATCGCGTGATGGGCATGTTCCAGGGGGCGTTCGGCTCGACCGCCGTGGCGGACGCCCGCTCCGCCGTGCCCGTCCCGCCCGGCTGGACCGACGAGCAGGCGGCGGCCGTTCCCATCGCGTTCCTGACCGCCTGGCACGGGCTGGTGGACCTGGCCGGTCTGAAGGCGGGCGAGTCGGTCCTCGTCCACGCGGCGGCGGGCGGCGTGGGCATGGCCGCCGTGCAGATCGCCCGCCACCTGGGCGCCGAGGTGTACGCCACCGCGAGCCCCGCCAAACACCACG TACTGGAGGCCATGGGCATCGACGCCGCCCACCGCGCCTCCTCTCGCGACCTGGACTTCGAGGGCGCCTTCCCGAAGGTCGACGTGGTCCTCAACAGCCTGGCCGGCGAGTTCACGGACGCCTCGCTGCGCCTGCTGGCCGACGGCGGCCGGTTCGTGGAGCTGGGCCTGACCGACGTCCGCGACCCCGAGCAGCTCGCCGAAACGCACCGCGGCGCCCGCTACCGCGTCCTCGACCTCGGGGCACCGAGAGAGGAAGGACTCGGCCGGATCCTGACCGAGGTGCTCGCCCGGTTCGCGCGCGACGAGCTGACCCACCCTCCGGTGCGCGTTTTCGACATCCGCCGGGCGCGCGACGCGTTCCGCCTGATGAGCCGGGCCGCACACATCGGGAAGATCGTGCTGACCCTGCCCCGGCCCCTCGAACCGGAGGGCACGGTCCTGGTCACCGGTGGCACCGGTACCCTGGGCGGCCTGATCGCCCGCCATCTGGTCACCCGACACGGGATACGCCACGTGCTGCTGACCGGCCGCCAGGGGCCGCAGGCGCCCGGCGCCGCCGAACTGCGCGAGGAGCTCACCGCGCTGGGCGCCACCGTGACCGTCGAGGTCTGCGACATCGGCGACCGCGACGCCGTCGCCGGGCTGCTGGCCGGCGTGCCCGACGCACACCCGCTGACCGGTGTGGTGCACTGCGCCGGCGTCCTCGACGACGGGATGGTCGACTCGCTGACCCCCGAGCGGCTGGCCCGGGTCCTGCGGCCGAAGGCCGACGGCGCGGCGCACCTGCACGAGCTCACCAAGGACGCCGATCTGGCGATGTTCGTCATGTTCTCCTCCATCGTCGGGGTGAACGGCAACCCCTCGCAGGCCAACTACGCCGCCGCGAGCGCCTTCCTGGACGCCCTGGCCCACCACCGCCGGGCCCACGGCCTGCCCGGTCAGTCCCTGGCCTGGGGCATGTGGGAGGAGACCAGCCGGCTCACCGGCCGGCTGGACGACGGGGACCTGGAGCGCATCCGCCAGGCCGGCATGGACCCGCTGCCCACCGAGCAGGCCCTGGCGCTGTTCGACCGGTCGTACGCCGCGGGTGACGCGCTGCTGGTTCCGATCCGGCAGGCCACGGCGGCCTCCGGCAGCCGCCCGGCCGCCGGCCGTGCCAGGGCGCGGCGCGTGGCCGACAGCGGGGCGGCGGGCACCGGCGGCCCGTCGCTGGCGGAGCGGGTCACCGCACTGCCGGAGGCGGAACGCGAGGACTACGTCCTGGAGGCGCTGTGCGCGCACATGGCGGCCGTCCTCGGCCACGGCTCGGCCGACGAGATCGCCCACGACCGCACGTTCAAGGAGCTGGGCTTCGACTCGCTGACCGCGGTCGAACTGCGCAACCGGCTCTCGGCCGCGACCGGCCTGCGCCTGCCCGCCACGCTCATCTTCGACTTCGCCCACCCACTGGCCCTCGCCCAGCACCTGCGCGAGCGGATCGCCGCGCCCGCCGACCCGGCCGGGAGCGCACCCACCGACCCGGCCGGGAGCCCGCCTCCGGACCCGCGTGAGAGCCGGGTGCGCGAGGTGCTGGCCGCCGTCCCGCTGCGGCGGCTGGAGGAGTCCGGCCTGCTCGACGCGCTGCTGCGACTGGGCGAGGAACCCGATCCCACCGACAGCACCGACACCGCCGACACCACCGAAAGCGCAGACAGTGCGGACAGCGCCGATTTCGCCTCGATGGAGCTGGAAGAACTGGTGGACATGGCCCTGCGCAACGGCGATTCCTGACGTGTGCCGTCCCCTCGGAGGAATGTCATGACGGTGGCCAACGACAAGATCGTCGAGGCCCTGCGCGCCTCGCTGCGGGAGGCCGAGCGGCTGCGCAAGGAGAACGACCGACTGCGCGCGGCAGCCCGGGAACCCGTCGCGATCATCGGGATGGCCTGCCGCTACCCCGGTGGCGTCGAGTCCCCGGACGACCTGTGGACCCTGCTGACGGAGGAGCGGGACGCGATCAGCGCCTTCCCGCGCGACCGGGGCTGGGACCTGGACGGCCTGTGCGGTCCCGACGCCCACCCGGCGGTGCGCAGCGCGGTCGCGGAGGGGGGATTCCTCGGCGGCGCCGCCGAGTTCGACCCCGGGCCCTTCGGGATCTCGCCGGACGAGGCGTCGGTGATGGACCCCCAGCAGCGGCTGCTGCTGGAGCTTGCGTGGGAGGTGTTCGAACGGGCCGGGATCGACCCGCGGTCGGCCGGCGGCCGGCGCTACGGCGTCTTCGTGGGCACCACCGGACAGGACTACACCCCCCACCTGAAGGACGTCCCCGACGAGCTGCTCGGCCACCTCGCCTCCGGCGGTTCGAGCGCCGTGCTCTCCGGCCGTATCGCCTCCGTCTTCGGCCTCGAAGGGCCCACGGCCACCCTCGACACCGCCTGCTCCGGCTCTCTGGTGGCGCTGCACCTGGCCTGCCGGGCGCTGCGCGGCGGCGAGTGTTCCATGGCCCTGGCCGGCGGTGTCACCGTGATGTCCAGCCCCGACGCGTTCATCGGCAGCGGCCGGGGGATAGGGCTGCCCGTCGCGGCCCGCTGCCGCTCGTTCGCCGACGGGGCCGACGGGATCGCGTTCGCCGAGGGGGCGGGCGTGGTGCTCCTGGAGCGGCTCTCCGCCGCCCGCGCGCACGGCCACCCGGTGCTCGCCGTCGTCCGTGGCAGCGCGATCGGTCAGGAGGGCGCCACCAACGGCGCGAGCGCCTCCAACGGCCCCGCCCAGCGAAGGCTGATCAGGCAGGCGCTGGCCGACTCGGAGCTCGAGCCGCACGAGGTCGACGCGGTCGAGGGCCAGGGCACCGGCGGCCTGCTCAGTGACGCGGTCGAGGCGCGGGCGCTGGCGTCCGTCTACGGCGAGGGCCGCCCCGCCGACCGGCCGTTGCCGCTGGGGTCGGTGAAGTCCAACCTCGGGCACACGCAGGGGGCCAGCGGTGTGGCCGGGGTGATCAAAATGGTCCAGGCCATGCGCCATGGCGTGCTGCCGCGCTCCCTGCACACCGAGGTGCCGTCACCGCACATCTCCTGGGAAGAGGGCCGGATCCGGCTGCTCACCGAGGCCACCCCCTGGCCCGCGACGGACCGGCCGCGGCGGGCCGGGGTGTCCGCGTTCGGATTCGGAGGCACGGACGCACACGTGATCCTGGAACAGGCACCACCTCCGGACGATCCCCCGCCCCCGCTGCCGGAGGCGGGACGCGGGCCGGCGGCCGCCGGTGTGGTGCTTTGGCCGGTCTCCGGCTGCGACCCCGACGCGCTGCGCGGGCAGGCGACCCGGCTGCTCGCCCACCTCGACCGGCGGCCCGAACTCCGGCCGGCCGACGTCGGCCTGTCGCTCGCGACGTCCCGGGCCGCGCTGACACACCGGGCCGTGATCGTCGGCGAGAGCCGCCAGGAGCTGCTGGACGGCCTGCGGGCGCTGGCCGGGGGAAGGGGCTCGCCCCGCGTCGTCCAGGGCTCGGCCGGACGCCGCCACCGGCTCGTCGTCCTGCTCAC CGGCCACGCCCTCGCGCCCGGCACCGGCAAGCAGCTGTACGACGCCTTCCCGGCGTTCGCCGACGCGTTCGACGCGGTGTGTGCGGAGCTGGACGCCCCGGTGGGATCCGAGGCCCGTGACGCGATGCTCACCGGCCCGGCCAGGGACTTCGCGATCCAGGTGGCGCTGTTCCGGCTGGTCGAGTCGTGGGGGGTACGGCCCGGCGTCGTACGAGGCCACGGCGTCGGCGGGCCGGCCGCCGACCACCTCGGCGGGCGGCTGACACTGCCCGAGGCGTGCGCGGCGCTGACCGGCTCGCCTCACCCGGCGGACACCCCGCGGGACTTCGCCGCCGCGGCCCGCACCCTGGCCCGGGAGGGCACGGCCTATCTCGAACTGGGCGGCCATGAGGTGGCCGAACTCCTCGACGGCGAGGGGCTGCCCGCGGTGGCCGCGCTGCGGCCCGGAGCGTGGGAGGTGGCCGCCGCGGCCGCCGCCCTCGCCCATGTGCACGCCCACGGCACCGCCGTGGACTGGCAGGCGGTGTTCGCGGGCGCCGACGCCCGCCGGGTCGACCTGCCCACGTACGCCTTCCGGCGCGCACGCTACTGGCGCGCGGCGTTCGACCTGAGCCGGGTCAACGGGGCGCGGTGAGACCGCCCGGAGCGGGGTGAGGGGCCGCACCGACCGGTACGGCCCCTCACCCCGCTCGGACGGCTCTCAGGCTTCGGCGCCCAGCTTCACCGGCAGCCGACCGAGCTGGCGAGTGCCCGGAACGGGGCGCCACGTCAGCTCCTTCTCCGGCACGGCCAGGGTCAGCTCCGGGAAGCGGCCGAAGAGCGTCTCCAGCGCCAGCTCCGCCTCCTGGTTGGCCAGCATGGCTCCCAGGCAGTAGTGCGGTCCGCGTGAATAGGCGACGTGCTGCGCGCCCCCACCGCCGGAAGAGCGGGTGATGTCCAGCCGCTCGGGGTCGGGGAAGCGCCGCGGATCGTGGTTGGCGGCGCCCTGCACGGGCAGGACGACCTCGCCCTTGCCGATCGGCGCGCCCGCGACCACGACATCCTCCGTGGCGTACCGCGGCTTGCCGAAGACGATCGGACTGCACCGGCGCAGCAGTTCCTGCACGGCTCCGGGCAGCAGCTCCGGCCGGCTCCGCAGCAGCGCCCGCTGGTCGGGGTGGGTCAGCAGCGCGAGGACGCCGTTGCCGATGAGACCGGCGGTCGTCTCGTGCCCGGCGACCACCAGGTGGAACAGCATGGTGACCAGTTCCGTGTCCGACAGCCGCCCGCTGTCCTCGCTCCGCACCTGAATGAGCGCGGTGATCAGGTCGCCGGCCGGCTCGGCGCCGCGCCGCTCGACCAGCGAGCGGAGGTAGGCGGTCGACTCCGAGAGCACGCGGCTCAGCGAGAGGGCGTCGGTGTAACCGTGGCTCCACTCGCGCCACATCGCCCTGTCCTCGGCCGGCACGCCGATCAGCTCGCAGATGACCGCGATGGGCAGACGGTGGGCGAAGTGCTCCATCAGGTCGACCGTGCCGTCCACGGCGCGGCCCGGCAGCCCGTCGACCAGCTCGTCCGTCAACGCCCGTATCCGCGGCTTCAAGTCGGCTACCCGCCGCGCCGAGAACGCCCGCGCGACGAGCGTGCGCAGCCGCGTGTGATGCGGCGGATCGAGCTGTGTGATGGCCTCGGCGAGATGGGGAACCAGGTCCTTGGACAACCCCAGCCAGCGCAGCGACTCGGCGTGGTGATCCGTCGTACATCCCGGAATCGACCGCGAGTTGCTGACGAAGCGCTCGTCGGTGAGCACGGAGACCACGTCCTCGTAGCGGGTGATCACCCACGCGTTCCGGCCGTCCGCCACGCGCGCCAGGGCGACCGGCGCCTCCTCCCTGATCCGCCCGAAGCCGCCGTACGGATCGGCGGTCAGCTCGGCCGCGTTGATGGCGAACGGGCAGCGGTCCGGCTCACCGGGCGTCTCGGCGTCACCTGCTGGTGTCATCTTCGGGCCTTCCGTCGTCGGCCATCCCTGCGGGTGATGATCGCCCATCCCGCTTAAGCAGTCCTGTCACCGGGGCGGGACCCGGGGCGGGACCCGAGGCGGGGCGCGGGCCGGTTCGGTAGGTCCGCTTAAGAGCGCTCGGCGAGCATGAGCCCTTGCCATTCCCGTTCCGAAATCACCGCTCCGTTGGTCTCAGCGATGCGGGGAAGCAGGGAGAACACGGTGATCAACGAGCGCAACCGCAAGTCGATCGCACGCGAACACAGCCGGCGCCTGAGTGCGGGCGATGTCGACGCGCTCCTGGAGCTGTACGCGAAGGACGCCACGTTCGAGGACCCCGTGGGGGCCGGCGGCCGGGCCGGACACGAGGCGCTGCGCGCGCACTTCGAGAGCGCGGTGGCCGGGAACGTCGCCGAGACCGTCGAGGATACGGTGGTCGGGCAGGACGGCGCGCATGTGCTGTCCCGGATCACCGCGGTCATGGACTACCGGCCGCGCGGCCCGGTGTACGCCGACCGCGGCTGGCTGCCCGCGCCGCAGGCCGCGGAGCCGACCGGACTGCGCTGCCACTACGCGCTGCTGCTCCGCGTCGGCGAGTCCGGCCTGATCGAGGACATGCGGGCGTTCTGGGGCCGGCCCGACATCGAGACATCGGCCGACGGCGCGGCGGAGTCCTTCCGCGGTCCCGAGTCCATCACCCCCGACGAGGCGGCTCTGCGGCAGCTGCCGTACACCTACGCCCGGCGGCTGGAGTCGGGTGATGTGTCCGGGACCGTCGCCCTGTTCTCGGACGATGTCGTCTTCGAGGACCCGGTGGGGCGCATGAAGCTGTGCGGCAAGGAAGCCCTGGCCAAGCACGTGGCCCACGGCTCGGCGGGCAAGGTGAACGAGATCCTGGGACGGCCCGTGTCCTCGATGGACGGACGGTTCGTGGTGATCCGGGGGGACGCCCGGATCTTTGTGCCGGAGAAGATGCGCATGCGCATGATCACCGTCTGTGAACTGAACAAGGACGGCCTGGGCGTGCACATCCAGGGCTTCTGGGGGATCACGGACACCACCTTCGGGGACATGGGGCCGGACACGGACACGGACACCGACCGCTGACCGGCGCCCGACGCCCGGCGCCGAGGAGCGGCCGGAAACGGCAAGACGCACTTAAGCCGCTTGGGACACCCTCGTGACGGTGTCCGAGGCGAAAGACGAATGCCACATCAGGCGAGCCCGTTTCGGAGGGACTTCCGCGCCATGACCACACCGACACGTGCCGTCGTCCTGGGCGGCGGATGGGCCGGCATGCTGACCGCCCATGTGCTGGCCCGCCATCTGGAATCCGTGACCGTGGTGGAGCGTGACCTCCTCCCCGACGGACCCCTCCACCGTAAGGGTCAACCACAGGCCCGCCACGTCCACATACTCTGGTCCAGCGGCGCGGGCATCGTCGAGAACCTGCTGCCGGGCACCATCGAGCGGCTGCTGGCGGCCGGCGCCCGCCAGATCGGCTGGAACCGCGACCTGGTGACCCTGACCGCCTCG GGCTGGCAGTACCGCTTCCCGGCCACCACCTACGCCATGATGTGCACGCGTCCCCTGCTCGACTGGGTGGTGCGTGACGCGATCCTCTCCGGTGGCCGCATCGAGGTGAAGCAGGGCACCGAGGTCGTCGGCCTGGTCGGAGACCGCTCCAAGGTCTCCGGAGTGCTGGTGCGTGACGTGGCCGGCGGCGAGCCCCGGCCGCTGGAGGCGGACCTGGTGGTGGACGCCACCGGGCGGGCCTCGCGCCTGGGCCGCTGGCTGGCGGGGCTCGGCCTGTCGGCCGTCGAGCAGGACGTGGTCGACGCCGGCGTCGGCTACGCGACCCGCATGTTCAAGGCGCCCGAAGGCGTGAGCGGCACTTTCCCCGCGGTCATGGTCGCCGCCGACCACCGCACCCGCAGCCCCGGCCGCTTCGGCGTGGTCTACCCCCAGGAGGGCGGCCGCTGGCTGGTCACCCTCACCAGCACGCGTGGCGCCCCGCTGCCCACCCACGAGGACGAGTTCACGGGCTTCGCCAAGATGCTGCGGCACCCGATCGTCAGCGACCTCATCGGCGTGGCCGAGCCCATCAGCCCGATCTTCCCCTCGCACTCGGGAGCCAACCAGCGCCTCTACCCCGAGCGCATGGCGCAGTGGCCGCAGGGCCTGCTGGTCATCGGCGACTCGCTGGCGGCGTTCAACCCCGTCTACGGCCACGGCATGAGCACGGCCGCGCGCGCCGCCGAGGCCCTGGACAGGCAGCTGGCGGGCGACGACTTCGGCGAGGGCGGCACCCAGCGGGTCCAGCGGGCGCTGAGCGAGGTGGTCGACGACCCGTGGATCATGGCCGGGCTGAAGGACATCCAGTACGTCAACTGCCGCATCCGCACCGACGACCCGCGCCTGACCGACCCGGACACCCCCGAGCGGCTGAGGTTCTCCGACTACGTCGCCGACCGGTCGATCCGCTCGCCGAAGGTCAGCGAGGTCGCCACCAGCGTGCTCAGCCTGAACACGCCGCAGAAGGCCCTGGGGGACTCCCACTTCCTGTCGCTGCTGCGGACCGACACCAAGTACCCGGAGCTCGTCGAACCGCCGTTCCACCCGGACGAACTGGAGATGGTGGGGCTCAAGCCGCCCGGCAACGCCGCGAACGGCGTGCTCGGATAACCCACCCCGCAGGCCCCACATCGAGAGAGGTACAGCCATGGAACCCATCGAAGAGTCGACCGGCGACAGCCGACTGGCCGAGCGGCTCGCGACCGCGACGCCGGAGGAGCGCGAGCAGCTCCTGCGCGGGCTGGTGCTCCGCCACGCCGCCGAGATCCTCGAAGCTCCGGAACTTGACGAGGAGAGCAACTTCGTGGAGAACGGCCTGACCTCGCTGCGGGCCCTCCAGCTGTCCAAGGCCCTGATGAACGACACCGGGCTCGAAGCCCCCCTCGTCGCGATCGTCGAGCACCCCACGCCCACCCTGCTCGGCAAGTTCCTGTCGGAGACCTACGAAGCGGACGCGGCCTAGGCCTGCACCCGTCTTGTCCTATCTCCAGGAGGCAGGGGATTTCCGCCATGAAGCTGTCCGTTGCCGTGGACCACGGAGTGACGTTGCGGGTGCGGCACCGGCCGGGCGGCGACGGCCGGCCGTTCCTGCTGCTCCACGGGCTGAGGTCCAACGCCCGGATGTGGGACGACGTGGCCGACCGGCTGGTGGCCGCCGGCCATCCCGTCTACGCGCTCGACATGCGCGGGCATGGTGACTCCGACCTGCCGGAACACGGATACGACAACGCGACCGCCACGGCGGATCTGGTCGCCGTCTGCCGCGAGCTGCGGCTGACCGGGGCGCTGCTCGCCGGTCACTCCTGGGGCGGGAACCTCGCGGTGCGGCTCGTGGCCGAACACCCCGAGGTGGCCGCCGGTCTCGCGCTGGTGGACGGCGGCTGGATCAGCATGGTGGACACCTCTCAGGGCGGCCCCGCCCGGGAGAAGGTCGTGGAGATGGTCGGGCGGGCACACTCCCAGGTTTCCGGGATGACCGCCCAGACCCTGCGGGAAACCCTGCGCGGCCTCCACCCGACCTGGTCAGCTGCCGCCGTCGAGGCGACCCTCGCCGACCTGGCCGAAGGGCCGGACGGGCTGCTCGTCCCGCGGCTGCCGCTGGAGAAGCTGCTGTCGATCCTGGAGAGCATGTGGGAGGACCCGCCCGCCCGCTGGTATCCCGCCATCACGGTGCCCGTGATGCTGTGCAGCACCCTGCCCGTGCAGGTCCCCATGCTGAGCGACCTGGCGCGGAAGTGGGTGGCGGAGGTCGAGGCGGCCCTCCCGCAGGCGGACCCCCGCTGGTACGTGGGCGCCGACCACAACGTGCACGCGGATCACCCCGCGCGGGTGGCCGGCGACCTGCTCGACCTGGCGGCCACCGTGGACAAGCCGGCATCGGAAAGGCCCTGACATGGCGAATGACGAGACGAAGCTCCTCGGCTACCTCAAGCGCGTGACGGCCGACCTGCGCGAGGCCCAGCGCCGGCTCAAGGACGTCGAGTACGCCCAGCACGAGCCCGTCGCGATCATCGGCATCGGCTGCCGGTTCCCCGGCGGGGTGAGCTCCCCCGACGACCTGTGGGACCTGGTCGCGGCCGGCCGCGAGGGCATCGGGGGCCTGCCCACCGACCGCGGCTGGGATCTCGAAGCGCTCTACGATCCCGACCCCGACCACGCGGGCACCAGCTATGTGCGCGGGGGCGGATTTCTCTACGGCGCCGACCGGTTCGACGCCGGATTCTTCGGGATCGGTCCGCGCGAGGCGGTCACGATGGCCCCGCAGCAGCGGCTGGCGCTGGAGGTGGCCTGGGAGGCCATCGAGCACGCCCGCATCGACCCGCACACCTTGCAGTCCAGCGCCACCGGTACCTACCTCGGCTGCGACGGCCTCGACTACTTCCTCAACTCCCTCCAGGTGCCTGAGGGCTCGGCGGCCCATCTGACGACCGGCAACTCGCCGAGCGTGGTGGCCGGTCGGGTCGCGTACACCCTCGGCCTGGAAGGCGCCGCGGTCACGCTGGACACCGCATGTTCCTCGTCCCTGGTCGCGATCCACCTGGCGTGCCAGGCGCTCCGGCAGGGGGAGGTGACGCTGGCGCTGGCCGGCGGGGTGTGCGTGATGTCCTCACCGGCACCGCTGGTCGGCTTCAGCGAGATGCGCGCCGTGGCGCCGGACGGGCGCTCGAAGCCCTTCTCCGCGGACGCCGACGGCATGACCCTCGCGGAGGGGGCCGGGGTCGTCCTGCTGGAGCGCCTGTCGGACGCCCGCCGCAACGGGCACCGGGTGCTGGCGGTCGTCCGCGGCACCGCCGTCAACGAGGACGGCGCCAGCAACGGTCTGACGGCGCCGAACGGCCCGTCGCAGCAGCGCGTCATCCAGCAGGCGCTGGCCAACGCCCGCCTCGCGGCGGCGGATGTGGACGCGGTCGAGGCGCACGGGACGGGCACCAGCCTCGGCGACCCCATCGAGGCCCAGGCGTTGCTGGCCACCTATGGGC ACGACCGGCCGGAGGGGCGGCCGCTGCGGCTGGGGTCGATCAAGTCCAACATCGGCCACACCCAGATGGCCGCCGGTGCCGCGGGCGTGATCAAGATGGTCATGGCGCTGCGCCACGAGCTGCTGCCGCGCTCCCTGCACATCGGTGCGCCCAACCCCCATGTGGACTGGGGCAACGGCGCGCTGAGCCTGCTGCGGGAGGCCGTCGCGTGGCCGCGCGACGCGCGGCCCCGGAGGGCCGGCGTGTCCTCCTTCGGATTCTCGGGGACCAACGCGCACCTGATCCTGGAGGAGGCGCCCGAACCTCCCCCTGGGGACCCCGGCGAGCCCGGCGAGCCCGGCGAGGCCGCCGATCGGCCCGAGGGCCGGGTCACGCCGTGGGTGGTGTCGGCACGCGGCGCGAAGGCGCTGCGCGGCCAGGCCCGGCGGCTGCTCGACGCGACGGCCGTCGTCGGCGGCGAGCCGGAGGCGGTCGGCTGGTCGCTGGCCACCACGCGCGCGACGCTCGCCGACCGGGCGGTGATCACGGGCCGGGACGCCGACACCCTGCGGGCGGGCCTGGCCGCGCTGGCGGCCGGGGAGGACCATCCGGCACTGGTACGGACCGCCGCCGCGGCGCCCGCCCCCGGGTGGCGGGTGTTCCTCTTCTCCGGGCAGGGAAGCCAGCGGCCGGGTATGGGGGCCGGACTGTACGAGCGTTTCCCGGTGTTCGCCGCCGCCTTCGACGAGGTGTGCGCGCTGCTCGATCCGCACCTGGACCATCCGCTGCGCGACATCGTCCTCGCCACCGACGAGGAAACCGCCGGCCTCGTCCACCACACCACCTATACGCAGACCGGGCTGTTCGCGGTGCAGGTGGCGCTGGCACGGCTGCTGCACGACTGCGGTGTGCGGCCCGACGCCGTGCTCGGCCACTCCATCGGCGAGATCGCCGCCGCACACGTGGCCGGAGTGTTCTCCCTGGAGGACGCCTGCCGTCTGGTCGCCGCCCGCGCCACCGCGCTCGGCGCCCTGCCGCCCGGCGGCGCCATGACCGCCCTGGAAGCCACCGCCGAGGAGGCCGCCGAAGCCCTCGCCGGCTACGACGGCCAGGTCGGCATCGCCGCCCTCAACGCCCCCACCAGCACGGTCATCTCGGGGCCGGCGCAGCTGGTGGCCGAGGTCGGCGCGGCCTGGAAGAAGCGCGGCCGGAAGGCCAAGGCGCTCACCGTCAGCCACGCCTTCCACTCCTCCCTCATGGAACCGGCCCTCGACGGCTTCCGCGACGCCATCGCCGACCTGGCCTTCCACCCACCGCTCATCCCCCTGATCAGCAATCTGACCGGGCAGCCGGCCGGCGAGGACATCGCCACCCCCGACTACTGGGTGCGCCACATCCGCCAGCCCGTCCACTTCCACCCCGCCGTCGCCCACGCCGCCCCGCAGACCGCCGTCTACCTCGAAATCGGCCCCGACCCTGTCCTCATCCCTCCCGCCCAGGACACCCTCGACACCCTGCGGGACGCCCCTGGGGCCACCGCGCCGCTGCTGATCCCGACGCTCACCCGCACCCAGCCCGACACCCAGGCCCTGGCCCAAGCCCTGGCCCGGCTCCACACCCACACCCCCGTCGACTGGAGCCCCTGGTTCACCCACCCGGCCCCGCCGCCCACCGTCGACCTGCCCACCTACTCCTTCCAGCGCGACCGCTACTGGCTGGCCCCGGCCCGTCCGACCGGTGGCCACGGCGTGCAGGATCCGGCCGAGGCCGAGCTGTGGGAAGCCATCGAGGACCACGACGTCGAGACTCTCGCGCAGGTGCTGGAGCCCGACGGCGAAGCCGAAGACATCGAGGCGCTGCGGCCGGCCCTGCCCGTGCTGTCCGCCTGGCGCCGCCGCCACCGCGAGCGCGCCACCCTGGCGTCGTGGCTCCACCGGATCCGGTGGACGCGGCTGCCCGAGCCCGACGCGCCGGCGCTGAGCGGCACCTGGCTGCTGCTCGTCCCCGCCGGCCACGAGAACCACCCGGCCGTACGGACGGCGCTCCAGGCCCTGACCGAACACGGCGCCGACGCCACCACCTGTCCCGTCGATACCGCCACCAGCCGTCAGGCGCTCGCCGAACGCCTCACCGCCCTGCGCTCCGAAGCCACGCCGTCGACCGCCCCCGCCGGCGTGCTCAGCCTGCTGGCCCTCGACGAAGCCCCGCACCCCGATCATCCGGCCGTGCCCGCCGGGCTCGCCGCCACCCTGACCGCCCTCCAGGCGCTCGGTGACTCGGACATCACCGCGCCGCTGTGGTGCCTCACCCAGGGGGCGGTGTCCACCGGGCAGGACGACCCACTGCCCCATCCCCTCCAGGCGCAGAGCTGGGGCCTGGGCCGGGTCGCCGCGCTCGAACACTCCCAGCGCTGGGGTGGCCTGGTGGACCTGCCGGTCGACGTCGACCAGCGGACCCCGGCCCGGCTGGCGGCCGTGCTGGCCGGCGCGCCGGCGGGCGCCGAGCCCGAGGACCAGGCGGCGATCCGCACCACCGGGGTGCTGGCGCGCCGCCTCGTCCACAGCACCACCGGGCACGCCGGGCCCGGCCGCGACTGGCGGCCCCGGGGCGCCGTGCTGATCACCGGCGGCACCGGTGGCATCGGCGCACTGCTGGCCCGCTGGGCGGCCGAACACGGGGCCGCCCACCTGGTCCTCATCAGCCGCCGCGGCCCCGACGCGCCCGGCGCCGGGGAACTCGCCGCCGAACTCAGCGGCCTGGGCGCCACGGTGACCCTGGCCGCATGCGACGCGGCCGACCGCGAAGCCCTGCGCGCCGTCCTGGACGCGATCCCCGCCGAGCACCCGCTGTCCACCGTCATCCACGCCGCGGGCGTCAGCGAGCACGACGCCGTCGCCGACGCGGACACCGGACACCTGAACCGGCTGCTCGAACCGAAGGCGCTCGCGGCCCGGCACCTCCACGAACTGACCCAGGACCTGGACCTCTCCGCGTTCATCCTGTTCTCCTCGGTCTCCGCGGCCTGGGGCAGCGGGGGGCAGAGCGGCTACGCCGCCGCCAACGCCTACCTGGACGCGCTCGCCGACCACCGCCGCGGACTCGGCCTGCCCGCCACCTCGGTGGCCTGGGGGCTCTGGGGCGAGGTGGGCATGGCCACCGCGTCCGACGACGTCGACTACTTCCGCCGCCGCGGCCTCCACCCCCTCGACCCCCGGCTGGCCGTCGCCGCCCTGGAGCAGGTGGCCGGAGGCAGGGAGACCAGTGCCGTGGTCGCCGCCGTCGACTGGCGGCGCTTCCTGGAGAGTTTCACCGCGCTGCGCCCCAGCCCGCTGCTGGGCGATCTCCCCGAGGCCGCCGAGGCGCGCACGGAGGAACCGCGACCGGCGGCCGAGGAGACGGACCAGCTCAAGCGGAAGCTCTCCGGCAGCGCGCCGGCCGAGCAACACCACATCCTGGTGCGGCACGTACAGGCACATGCCGCCGGTGTCCTCGGCCACGCCGCCGCGGACGACGTCCCGCCCACCAAGCCCTTCCAGGAGCTGGGCT TCGACTCGCTCAACGCCGTCCAGCTGCGCGACCGGCTCAACGCCAGCACCGCGCTGCACCTGCCCACGACCGTGCTGTTCGACTACCCGTCGGCCGACGAACTCGCCCGCCATCTGTACGGCCTGCTCGTCACCGACGGGGCCTCGGGCGAACAGCGGGTCATGTCGGAACTCGACGGCTGGGACGTCGCCCACGCACCGGACGCCGTGGACGAGGCCGCCCGCTCCCGGGTCGCCGCACGGCTGCGGCTGCTGGCCGACAAGTGGGCCGACCCGGGCCGCGCCACCGGGACCCACGACGACCTGGAGACGGCGACGGCGGAAGAGATCTTCGACCTGATCACCCAGGAGTTCGGGAAATCCTGAGCGGGGCGCGGAAGAAGGGAGGGGTTCGTCAGCGGACTGACGAACCCCTCCCGAGGCTTTGGGCCCAGGGCCTCAGCGGGAGCCGTACTCGGCCGCCAGCTTCTCCAGCTCGGGGACCACGTCATTGGGGGACGGCTGAGCCGCCATCTCCTGACGGAGCCGCTCGGCCCCGGACCGGAACGACGGGTCCTCCAGCAGCCGGACCAGGTCCTCCCGGATCCTCGGCCCGGTCATGTCCTGGGGCGGAATCCACAGCCCGGCCCGGGCCTCCTCAAGACGTTGGCCCCGTATCTGGGCGTCCTGAGCGGTGAGGCTGACCATGAGCTGCGGGACTCCGTGCGCCAGTGCGCCGCAGAACGCCGGGACCCCACCGTGGTGCATCACAGCGGAACATGAGGAGATCACGGCGTGCAGGGGCACGAAATTGACGATTCTCGTGTTCTCGGGGACCCGCTTGAGTTTCCGCTGAATACTTTCGGAGAGCGTCAGCATCAGCTCGATGTCCAGGTCGGCCACCGAGTCCAGCATCTCCTGGATCTGGCCGACGGGAACGGGCTGGCTCCCTGCCACGCTCCACATGCTCACTCCGAAGGTGGCAAGGACGCGCGGCGCCGGCGGCTTCTCGTGAATCCAGTTCGGAATGACGGAAGGGCCGTTGTGCGGGACGTAGCGAACGGAAACGTTCCGCACATCGCTTTCCGACCTTATCGAGTCCGGCATCTGATCGATCGTGAACTGGCCGGTCAGCAGTTCTTCCGAGGCATCAAAGCCGTATTTCGACGCCCACTTGCTCATCCATTCTTCCACCGGGTCCTCGCGGTCCCCCGGCGGCTGCTGCGCCCGCACCGTCAGAAAGCGCTGACGCAAAGGCGTTCCCACGTCGATGGCGAGGTGCATCCGCGCATGCGGCACTCCCGCCGCGGCGGCGGCGACCGAACCGGCGTAGCTCATCGATTCCCAGACCACCAGATCGGGCTTCCACGAGCGGCAGTACGCCACCAGGTCGTCGACCATCGAGTCGTTCGCGGCCTCCATGATCATGGCGAAGACGGTGTGGAACGCCTTCAGGTTCTCCCAGGTGAGCTGCTCCTCGCGGGCGTAGTCGACCTTGGGCAGCACCATGCCGCTCCGCGCTGCCCGCTGGATGATCAAGTCCCCTTTTTCCTGGAGGGACGGACCCGATCCCGCGGCTACCGCCGTCAGCCCTGTTCCGGTGATGACGTCGGTGAATTCGGGGCTGCTCGCGACATGGACGTCATGGCCGGCGGTGCGCAGCGCCCAGGCCATGGGCACGGTGAGATAGAGGTGGGTTTTCCAGGGGAATGTGACGAACAGGACGCGCATGGCTACCGTCTCTCCTCACCAGTCGCCGTGGGCAACCGATCTCGCTCGCCTGATCCCAATATGCTATCGCCGCCCCTTAGGCGGATCTTACGGTCCGCTGTTAGCCGAACTTTAGGCAGGTGAGTGAGGATATGACGGAACCACAATGAGGTGGTGATATGTCGGCGTCGTCAGGATTCCATGATGAAGGGTATTGTTCTCGCGGGAGGAAATGGGACACGGCTGCACCCTCTTACCCTGGTGGGGTCGAAGCAGTTGGTTCCCGTTTACGACAAGCCCATGATCTACTATCCGCTGTCCGTTCTCATGTTCGCGGACATCCGGGAGATCCTCGTCATCACCCGCCCCACCGAAATGCCGCTGTTCCAGGAACTCCTCGGAGACGGTGAGCATCTGGGACTGAGCATCAGCTATGCCTCCCAGGAGAAACCGCGGGGGATCGCCGATGCCTTCCGGGTCGGCGCGGACCACATTCGCGGTGAGGACTGCGCGCTGATTCTGGGCGACAACCTCTTCCACGGCGCGAAACTGCCCACGCTGCTCCGCCGCAGCGCGGAGCGGCTGCGCGGGTGTGTGATGTTCGGGCACGAGGTCGCCGACCCCCGCCACTTCGGCGTCGCCGAGGTCGACGACCGGGGCCGGCTAGTGTCCATCGAGGAGAAGCCGGAGCGGCCCCGGTCGAACCTGGCCATCCCCGGCCTGTACTTCTTCGACGGCGGCGTCGTCGACGTCGCCAAGCGCCTGGTGCCCTCGGCCCGGGGCGAGCTGGAGATCACGGACGTCCTGCGCGCCTACCTGGAGGACGGCCTGGCCGACCTGGTGTGGCTCGGCAGGGGCGTCACCTGGCTGGACACCGGCTCGCACGAGACCCTCCTGGACGCCGGGCGCTTCGTACGCGACCTTCAGCACCGTCAGGGCACACGGCTCGGCTGCGTCGAGGAGATCGCCATGTACATGGGATTCATCGGGGCAGCCGAGTGCCACGCGCTCGGCGCCAGGATGAGCACATCGCCCTACGGCCAGTACGTCATGGACCGTGCGCTCGCATACCGGCAGGGGCTGCCGCCCGCGCGTTTCCTGGAGGAGCTGTGAACCTCCTCGTCACCGGGGCCGCGGGGTTCATCGGCTCCACCTATGTGCGCGGTCTCCTCGCGCGGGGCGGCGCGGAGCCGGACCCCGACGTGTCCAGCGTCACCGTGCTGGACAAGCTCACCTACGCCGGGACGCTGAGCAACCTCGAACTCGCCGATCCCCGGCTGACGTTCGTCCACGGCGACATCTGCGACGCCGATCTGGTCGACACGCTGATGGCACGGGCCGGCCAGGTGGTCCACTTCGCCGCCGAATCGCATGTGGACCGCTCCATCGACGGCGCCGACCCGTTCGTACGCACCAACGTCGAGGGCACCCACACGCTGCTTCAGGCCGCCCTGCGCCACCGGGTGGAGCGCTTCGTCCATGTGTCCACCGACGAGGTCTACGGCTCGGTCGAGACCGGCACCAGCCCCGAGACCGCGCCCCTGGACCCCAACTCTCCCTACGCCGCGTCCAAGGCCGCCTCCGACCTGATCGCCCTCGCCTACCACCGCACCCACGGCCTTGATGTGCGCATCACCCGGTGCAGCAACAACTACGGCCCCCATCAGTTCCCCGAGAAGATCATCCCGCTGTTCATCACCCATCTGCTCGACGGCGAGGACGTGCCGCTGTACGGCGACGGGCTGAACGTGCGCGACTGGCTGCATGTGGAAGACCACTGTGGCGGGGTGGAACTGGTGCGTACGAAGGGGCGTCCCGGCGAGATCTACAACATCGGCGGTGGCA CCGCGCTGACCAACCGGGAGCTGACCGGGCGCCTGCTGGACGCCTGCGGAGCCGACTGGAGCCGCGTACGCCACGTCGCGGACCGCAAGGGGCACGACCGGCGCTACGCGGTCGACGACGACAAGGCAAGGAACGAGCTCGGCTACCGCCCGCGCCACGACTTCGTGGCCGGGCTGGCCGAGACGGTCGCCTGGTACCGGCAGCACCGCACGTGGTGGGAGCCGCTCAAGCGCTCCGCCCAGCCCGCACACCGCTGACCTCAACCGACACACCGATGACCGCGCGGACCCCGCGCGGTCAACGGTGTGCGGTGGCGACGCTCAGCGGTGGCCTCCGACGAACTCGGCGACGGAGGCCACGACATAGTCGATCATCTCGTCGGTCAGCCCCGGATAGACCCCGACCCAGAACGTGTGGTCGGTGATGATGTCGCTGTTGGCCAGCTCGCCGCACACCCGGTGCGGCGCCTCGGCGTGCGCGGGATGGCGCGTCAGATTCCCGGCGAACAGCCGGCGGGTGCCGATCTTGCGGGACTCCAGGAAGTCGACGAGCTGGGCGCGGCTGAACGGGGCCCGCGGGTCGACGGTCAGGACGAAACCGAACCAGCTCGGATCGGAGCGCGGCGTCGCCTCCGGCAGGATCAGCCACGGCAGCCCGTGAAGACCCTCGCGCAGCCGCCGCCAGTTGCGCCGCCGGGCCGAGCAGAACGCGTCGAGCTTGTCGAGCTGGGTCAGCCCCAGCGCCGCCTGGAGGTCGGTCGACTTCAGGTTGTAGCCGATGTGGGAGAAGATGTACTTGTGGTCGTAGCCGGCCGGCAGCGTGCCCATCTGGTAGCCGAACCGCTTGCGGCAGGTGTCGCTCTCGCCCGGCTCGCACCAGCAGTCCCGGCCCCAGTCCCGGAGCGACTCCACGATCCGCGCCAGCACCAGGTTCGAGGTCAGCACGCAACCGCCCTCTCCCGTGGTCAGATGGTGCGCGGGATAGAAGCTGACGGTCGTCAGATCGCCGAACGTCCCCGCCAGTCGCCCGTCGTAGGTGGACCCCACGGCGTCGCAGGTGTCCTCGATCAGCACCAGGTCGTGGTCCTGCGCGAGCCGCGCCATCTCGGTGGCCTCGAAGGGGTTGCCCAGCGTGTGCGCCATCATGATCGCCCGGGTGCGCGGGCCGATCGCCTCCGCCACCCGCTCGGCCGTGGCGTTGTACGTCCCCAGCTCGACGTCCACATAGACCGGGACCATGCCGTTCTGGAGGATCGGGTTGACGGTGGTCGGGAAGGACGCCGCCACCGTGATGACCTCGTCGCCCGGGCGCAGCCGGCGGTCGCCCAGCAGATGGGAGGTCAGCGCCGAGAGCGCCAGGAGATTCGCGGACGACCCGGAGTTCGTCAGATGCGCCTTGCGCCGCTTCATCCGGCGCGCGAAGGCCGACTCGAACTTCCGCGAGGCCTTGCCCGCGGCGATCCGCATCTCCAGCGCGGCCTCCACCAGCGCGACCCGGTCGTCCTCGTCCAGCACCGCGCCCGAGGGCCAGATCTCGGTGACCCCGGGGATGAAGCCCCGCCCCTCCTGGCTGTCCTGGTGATACTTGCGCACCTCGTCGAGGATGCGCGCGACTCGTTCGCTCATCTGCTGTCCTTCCCATGGCTGTTCGGGGGTCCGCAGGCCGTCGCCGCCCGCACCGTCGCGGGCGGCGGCACGCCGGCGGCCGCGAGCAGATCGCGCACCGACTCCCGCAGGGCTCGCCGCGGCGACCAGCCCAGCAGGCGGCGCGCCCGGCCGATGTCCAGTCGTTGCCAGGCCGCGTCCGAACGTGTCCGGTGCAGGGCCGGGTCCTCGACGACGCGCACCGGCAGCCCGCTGAGGGAGATCATCAGGTCGACCAGCGCACGCACCGGCACCGCCCGGCCGCTGCCGATGTTCACGACACGGCCGGCCACCGCGTGGGACGGTGCCAGGGCCGCCGCGACCACCGCGTCGGCCACGTCCCGGACGTCCACCAGGTCGCGGTGGTCGTGCAGGGGCGCGAGCCGCAACTCGTCGCGCCCGGCCGCCAGGTGCGCGGCCACCACCCCGGCGAGGCTGTTGCCCGGGGCGCCCGGGCCGCAGGCCACGCCGACCCGCAGCACGATCCCGTCCACCCCCAGCTCGGCGGCGGCCCGCAGCACCGCCTGCGACCCGCGCAGCTTGGTGCGGCCGTACACGGTGGTCGGGGCGGTCGGCCAGTCCTCGCCGATCGACGTGCCCGGGTCGGCCGGGCCGTACTCGTAGGCGCTGCCCAGATGGATCAGACGCGGCCGGTCGGGCAGCCCGGCGACGGCCCCGGCCAGCCGGCCGACCAGTTCGGTGTTGGCCTCGGCCATCCGCCGTTCGCCACCGCCCCACACCGCGCCCGAGGCGTTCACCAGGACCCGCGCCCGGGTGTCGGCGCAGAGCCTGGCCAGCTCGGCCGGCTCGGCCTCGGTCAGGTCGAGACGCACCGAGCGGCAGCCGTCGCCGTCCACGCCGCCGTCCACGCCGCTGCGGGAGACCCGCACCACCCGCGCGCCCGCGGCGCCGAACGCCGAGCAGATGTGCCGTCCCAGATACCCGGACCCGCCCAGGACCAGGACCGGCGTCGTCATGCCGCTTTTCTGCTGCTTGTTTTCCTGAAGACCGCCTGCCGGAAGTAGCTCCAGGGAACGCCCCGCGCGTCGGCGGTACACGCCCATCCCGCACCGGGCCGATAGGCCTCGATGAAGGCGGGCACGGCCGCGTCCACGGCCTCGCTGTCGTGGATGTCGAGCACCTGCCGCAGCGGCCCGGTCTCCATGGCCAGCCGCACGGCCTCCTCGCCCTTCTGATGGCCGTCCATTGCCTTGTCCCGGAACTTCCCGACCGGATTCTTGACGAAGAACGCCTCGCACCTGTCGTCGATCAGACGCAGATAGGCCTGCACGGTGTCGGGGGTCATCTCCGTGAAGGAGTGCACATTGACGCACAGCTCGAAGCGGTCCGACCCCAGGGCGGCGTCGATGTCCTCGTCCTCGACCTGGATGAACCTCATCTTGGCGAACTGCTTGTCGTCCAGGACCTCGCGAAGATACGCCCTGCTCAGGGAGAGGGTGTTCTTCAGATCGACAATGGTGTAGGAGTCGAGGTCGTAGTTGGACAGCATCGCGTGGCAGGTACGGCCGTATCCCGCGCCGATCTCCAGCACGCGGGCGCCGTCCAGGGTCAGTTCCCTCTCGATGAATCCGAGTTCGAGCGCCGCCTGGAGATAGTCCAGGCAGATGCTCTTGCCCTCGTAGCGAACCGTGACCGGATCTCCCACGTCACGGTTGTGGACATTCTCGATTTTCGACCATTCGTCGTCACTCAGCCGCGTGGCCAGCTCGTAGACGAGGGCCCTCAGATACCTGACCCCGTTGGCCGTCGGGTCCCAAAGAGCGATATTGAAATTCCGTCGGTCCGACTTGAATCCCGTCAAGTCGGCGGCCGCGTCCTCAGTGACCCAGAGCTCGTTGATGCGCTCCCACTGTCGGCTGG CCTGAAAACCCTTCGCCATCCCGCCCTCCTACTCCATCGGCGATGGTCACATCACGCGGTACGAAGTCTGTCTCCCGTGTCTAATGGCGGGCTAACACTTCCGGGTGCCGTGGTCAGGACTCCCCGAATTCCCGGTCGATAAGGGCGAACACCTCATCCGCGGAAGCCGTTTCCAGGTCGTCCCCGCCCGTCTCCTCGCCAGGTTCGGACGCGCTCTCCCCGTCCGTGTAACGCCACAGCAGCACCTGTAGCCGGTGCGTCAGCCCGGAACGCCGCGGGTCGTCCGCGTCCAGCGCGGCGAGCGCCGCCTCGATCCCGTCGAGGTGCTCCGCGGCCGGTACCTCCGCCGGCTCGGCCGGCAGCACCCGCGACACCAGATGGCCCGCCAGCGCGGTCGGCGTCGGGTAGTCGAAGACCAGCGTGGGCGACAGGGTCAGGCCGGTGGCGTCCCGGAGCCGGTTACGGAACTCGACGGCCGTGAGGGAGTCGAACCCCAACTCGTTGAAGGGCTGGTCCGGTTCGACCAGCTCCGCCGCCGAATAGCGCAGCGCGCTCGCCGCGTGGGCGCGGACCAGCGCCAGCAGCGTGCCGGTGCGCGCCTCGGGCTTCTGGGCCGCGAGGCGGTCCCGCAGGGCCGTCAGGCTCCCGGAGTCGTCGGACTCGGGGGAGCCCGGCGCCGCCCGGCCCGCCCGCCGGGCCGCCGGGATCTCGTCGAAGAGGCGGCTGGCCCGCCCCAGGGTGAACAGCGGGGCGAAGCGCTCCCAGTCCACGTCCGCGAACACCGCGTGGGCCTCGTCGTGGCCGGCCTGATCGAGCGTCCGCCGCAGCGCGGTCAGCGCCAGCGCGGGGTCGAGGGCCGGGAGGCCATGGCGCCCGGAGCGTTCGACCGCCGCCCGCTCCGCCATGTCGTCCCACACGCCCCACGCCACCCGGACGACGGGCCCACCGGCCGCCTCGCGCCGCCGCGCCACCGCGTCCAGGTATGCGTCGGCCGCGGCCCGGGCGCCGTGCTCGCCGGCCCCCCAGACCGCGACGACGGACGAGAAGACCACCACGGGATCGTCGGGCTCCAGGCCGCACAGCTCCGGGAGCCGATCGGCGCCGCCCACGGCGGCCGACAAGGACTCGGCCAGCCGGTCCGGGGTGAGTTCGGCCAGCGGTGTCCGGTCACCCGGCGCCCCGGTGTGGATGATCGTGCGGACCCGCAGCCCCGAGGCCGCGAGCCGGTCCGCCAGTTCGTGCCGGCCCGACGGCTCGGACGGCACACCGTCCACCACCGTCGCCTCCGCGCCCAGCTCCGCCAGCTCGGCCACCAGCTCCGCGCCACCGGGCGCGGCCTGGCCGCCCGGCGCCAGCAGCACGATGCGCTTCGTGCCACCCGTGGCCAGCCACCGGGCCACATGGGCGGCGGCCCCGGCGACGCCGCCGGTGACCAGGACGCCCTCCCGCGGCGTCCACTCACGCGTTGCCGCCTCCGCGTCCAGCGGCGCGGGCAGCAGCCGGCGGGCGAAGGCTCCGGCCGGCCGCAGTGCCACCTGGTCCTCGCCGTCCCGCCCCGCCAGCGTGGCGCACAGAAGGCCGGGGACGAGGTCCTCGACCGAGGCGGGCAGGTCCACCAGCCCGCCCCAGGCCGTCGGATGCTCCAGCGCGGCCACCCGGCCGAGCCCCCATACCTGGGCCTGCGCCGGGGCCTGCGGGCGTTCGTCGTCGGCGACAGCCACCGCGCCTCGCGTGGCGCACCACAGGGGTGCGTCGAGCTCCGCCTCCAGCAGGGCTCGCAGCAGGACGAGGGTGCCCGTCACGCCGCCGATCACCGCGGGGTACGTTTCGTGCGGGCGTTCGTCGAGCGGCAGGAGGGACAGCACCCCGGCCAGGGACGGGCCCGCGGCGCACACCGCCCGCAGCGACGCGGCCAGCTCGGCCGGATCGGCCCGCCCGTCCGCCACCAGCCGCCGCACCTCGCCGCCGCCCGCCGTGAGGGCCTGCTCACAGGCGTCGGCCCATGCCCCGGCGCCTTCCGGCCCCACCACCAGCAGCCACGAACCCGCCACGGCCGCGGGGCCGGGGCCGGGCAGGGGGCGCCAGCCGATCCGGTAGCGCCAGGAGTTCACGGTCGTCCGCTCGTGGCGTTCCCGGTGCCAGCGCGACAGCGTCGGCAGCACCGCACCGAGCGACGAGCGCCGCCCGTCCTCCTCACCGAGGCCCAGCGTCTCGGACAGAGCGGGGAGATCCAGCCCGTCGACCGCGCCCCAGAACCGGACCTCCTCCTCGTCCGCCTCCGGCGGCGGCGCGTCCGTCAGCTCGTCCGGCAGCCAGAAACGCTCGCGTTGGAAGGGGTAGGTGGGGAGGTCGGTGGTGGTGGGTTTGGTGGCCGGGTACCAGGGTCGCCAGTTGACGGTGAGGCCGGTGGTGTGGGCGTGGGCGAGGGCGTCGGCGAGGTCGTGGGGGGTGCCGTGGTCGCGGCGGAGGGTGGGGATGGTGGTGGCGGGGATGTCGGCGTCGTCGATGGTGTCCTGGATGCCGGGGGTGAGGACGGGGTGGGGGCCGGCTTCGATGAAGTGGCGGTGTCCGCGGTCCAGGAGGGTGGTGAGGGTGTTGGTGAAGCGGACGGGTTGGCGGAGGTTGGTGAACCAGTAGTCCGCGTCCAGTGGGGTGGTGGTGAGGCGCTCGGTGGTGACGGTGGAGTAGAAGGGGATGGTGGGTGTGGTGGGGGTGAGGTCGGCGAGTTGGTCGAGGATGGTGTCGCGGATGCGGTCGACGTGGGGGCTGTGGGAGGCGTAGTCGACGTCGATGGTGCGGGTTCGGGCGCCCTGGGTCTCGGCGGCGGTCAGGACGGTGGCGATCTGGTCGGGGGGTCCGGAGATGACGGTGGAGCGGGGGCCGTTGTGGGCGGCGATCGCGACGCCCTCGGCAGTCTCACCCAGGTCGGCCAGGATGCGTGCGGTGTCGTCGGCGGTGAGGGTGAGGGAGGCCATGGCGCCGTGTCCGGCGAGGGCGCGGAGGGCCTGGCTGCGCAGGGCCACGACTTTGGCGGCGTCCTCAAGCGTAAGGGCTCCGGCCACGCAGGCGGCGGCGATCTCACCCTGGGAGTGGCCGATGACGGCGGCCGGGGTGACACCGAAGTCCTGCCACATGCCGGCCAGGGAGACCATCATGGCCCAGAGGGTGGGCTGGATGACATCGACGCGCTGGGGGTCGGTGGTGGTGTCGGTGCCGCGCAGCACGGCGGTGAGTGACCAGTCGACGTAGGGCGCGAGGGCTTGTTCGCAGGCGGCGATCCTGCTGGCGAAGGCGGGGGAGGAGTCCAGGAGTCCGACGGCCATGCCGGCCCATTGGGAGCCCTGGCCGGGAAAGACGAACACCGGGCCCTGTTGGGGGTCGAGGGCGGTGCCGCGGGTGAGGTGCGGGTGTTCGCCACCGTCGGCCAGGGCGTCGAGCGCGGCGAGCAGTTCGGCGGGATCGGTGCCGGTGACGACGGCACGGCGATCGAAGGTCGCGCGCGTGGTGGCCA GGGACCAGGCCACCTCGGTGGGCGAGACGCCGGGGTTGTCGGTGATGGTCCGGGTCAGCCGGCGCGCCTGCTCGGCCAGGGCCTCGTGGGTACGGGCCGAGAGCACCCAGGGCGCGACACCGCCCAGCACGCCCGGCTCGTCAGCGTCGGGTTCCGGTGCCTCAGGGGCCTCTTCGAGGATGACGTGGGCGTTGGTGCCGGAGATGCCGAACGCCGAGACGCCCGCGCCACGGGGGCGTTCGGCGGTCGGCCAGTCGACGGGCACGGTGAGCAGACGTACGGCGCCGCTGCTCCAGTCCACGTGTGAGGTGGGCTCGTCGACGTGGAGGGTGGCGGGCAGGGTGCCGTGGCGGAGGGCCATGACCATCTTGATGACGCCCGCGATCCCGGCGGCGGCCTGGGTGTGGCCGATGTTGGACTTGATGGAGCCCAGCCACAGCGGGCGGTCCTCCGGCCGTTCCTGTCCGTAGGTGGCCAGCAGTGCCTCGGCCTCGATCGGGTCGCCGAGGGTGGTGCCCGTGCCGTGCGCCTCGACCGCGTCCACCTGGTCGGCGGGGAGCCGGGCATCGGCCAGCGCCTGCCGGATGACGCGTTCCTGCGCGGCATCGTTGGGCGCGGCCAGCCCGTTGCTGGCGCCGTCCTGGTTGACGGCCGAGCCCCGGATGACGGCCAGCACGTGGTGTCCGTTGCGGCGGGCGTCGGAGAGCCGTTCCAGCAGGACCAGGCCCGCGCCTTCGGAGAAGCCCGTGCCGTCGGCCGCCGCGGCGAACGCCTTGCAGCGGGCGTCCGGCGCGAACCCCCGCTGCTTGGAGAACTCCACAAACGCCGTGGCCGCGGTGAACACCGTCACGCCGCCGGCCAGCGCCAGGGTGCACTCGCCCTGGCGCAGCGCCCGGACCGCCAGATGCGTGGCCACGAGAGACGACGAGCACGCCGTGTCCAGCGTGACCGCCGGACCCTCAAGACCCAGCGCGTACGCCACTCGGCCGGACACCACGCTCGTGAGCCCTCCCGTGGCGGTGTAGCCCTCGAAGCCCTCGGGAATCCGCGGTATCCGCGACAGGTAGTCCTGGCTCGACACCCCCGCGTAGACGCCGGTGGGCGTGCCCTTGAGGGTGGTGGGGTCGATGCGGGCACGTTCGAGGAGTTCCCAGGCGACCTCCAGGAGGAGCCGCTGCTGGGGGTCCATGGCGAGGGCTTCACGAGGGCTGATGTTGAAGAAGGAGGCGTCGAATTCGTCGGCGTCGTGGAGGAAGCCGCCCTCCTGGACATAGCTGGTGCCGGGGTGGTCCGGGTCGGGGTGGAAGAGGTGGTCGAGGTCCCAGCCGCGGTTGGCGGGAAAGGGGCCGAGGGCGTCGGCGCCCGAGGCTACGACGTCCCACAGGCCCTCCGGAGAGGTGACCCCGCCCGGGTAACGGCAGGCCATGGCGATGATGGCGATGGGTTCCCGGTGGCGTTCTTCCACTTCGCGCAGGCGCTGTCTGGCCTGGCCGAGGTCGAGGGTGACGCGCTTGAGGTACTGGCGCAGCTTGTCTTCGGTGGTGGACATGATTCCTCCGTCACTCCCCGTCAGGACGAGCCCAGTTGCTGGTCGATGAGCGCGAACATCTCGTCGTCCGACGCCGACTCCAACGCCTCGCCGTCGACGGTGTGATCGACCGCCTCGGCACCGTCACCCAGCCGCCACAGCAGGTTCTGCAACCGCTTGACGAGCTGGGCACCGGTCTCGCCCTCCGGCCGCACCTCGCCCACCGCCGCCTCGAGCCGCTCCAGCTCCCGCAGTACGGGCGCCGCCGCGCCGTCCCCCGCCGGGCACAGCTCGCCCAGCAGGTGCTCGGCCATGGCGGACGGCGTCGGGTGGCGGAACACCGACGCCGCGGACAGCCGCAGCCCCGTCACCATGGTGAGCCGGTTGCGCAGCTCGACCGCCGTCAGCGAGTCGAACCCGAGATCCTTGAAGGGCGTGTCCATCGACACCGCGTCGTGGTCCGTGTGCCCCAGCACGGTGGCCGCGTGGACCCTGACGAGGTCCAGCAGGAGCGCCCGCTGCTCGGCGGGGCCCATCGACGCCAGCCGGCCGGACAGATCGGCCACCGGCGCCACCGCACTCCGTCGGACGGCCCGGGCGCGGGTGCCCGCGAGCGAGCGGACCAGCGGCGGCAGCGCCTCGGCGGGCTGGGCGGACAGCGCGGCCGCGTCGAGCCGGGCGGCGAGCAGCTGCGCCGCACCGTGTCCGCGCGCCGCGTCGAACAGCGCCAGGGCATGCTCCGAGGACAACGGCACCATCCCGGAACGGCTCATCCTGGCGTGGTCCGCCTCGCTGAGCCCGCCGGTCATCCCACTGGTGGCGGCCCACAGGCCCCAGGCGATGGACACGCCGGTGAGACCCGCATGTTGCCGCCGGGTGGCGAGGGCGTCGCAGTAGGCGTTGGCGGCGGCGTAGTTGGCCTGCCCGGGGGAGCCCAGGGTGCCCGCGGCGGAGGAGAAGACGACGAACATGGCGAGGGGGAGGTGTTCGGTGGCGCGGTGGAGGTTGGTGCCGCTCGTGGCCTTGGCGGCCCAGACGCGGCGGAGGGTGTCGGGGGTCTGGGAGGTGAGGACGGCGTCCTCGACGATGCCCGCGGTGTGGATGACGCCGGTGAGGGGGTGGGCGGGGTCGATGGTGTCGACGATCTGCCGCAGGGCGTGGGGGTCGGTGGTGTCGGTGGCGGTGATGTGGACGGTGGCGCCGAGTTCGGTGAGTTTGTCGGTGAGTTGTTGGGCGCCGGGTGCTTGGGGTCCGCGTCGGCTGAGGAGGTGGAGGTGGGTGATGTGGTGGGTGGTGACGAGGTGTTCGGCGATGAGGGCGCCGAGGGTGCCGGTGCCGCCGGTGATCAGGACCGTGCCGTTGGGGTCGAGGGTGCGGGGGATGTCCAGGACGAGTTTGCCGGTGTGTTTGGCCTGGCTCATGTGGCGCAGCGCTTGGCGGGCCCTGGCGAGTGGCCATGCCTGCACCGGGGGTGGGGTCAGGGTCCCCTGGGTGAAGAGGCCGGCGAGCGTACGCAGCATCTCCGCGACGCGGTCGAAACCGGCGTCGGAGACGAGGTCGTAGGCGCGGTAGCGGACGCCGGGGTGCGTCGCGGCGATCTCATCCGGTTCGCGGATGTCGGTCTTGCCCATCTCCAGGAACCGGCCGCCGTCGCCCAGCAGGCGCAGAGACGCGTCGGTGAACTCGCCGGAGAGGCTGTTGAGGACCACCTCGACCTTCGGGAAGGCGTGCTCGAAGTCCAGGTCGCGGGAGGAGGCGCGGTGGGCTGCGTCGATGCCCATGGCCTCCAGGACGTGGTGTTTGGCGGGGCTCGCGGTGGTGTAGACCTCGGCGCCCAGGTGGCGGGCGATCTGCACGGCCGCCGTACCCACGCCGCCGGTCGCCGCGTGGATGAGGACCGATTCGCCCGGTGTGAGTCCAGCGAGGTCCACCAGCCCGTACCAGGCGGTGAGGTACGCGATGGGTACAGCGGCGGCCTGCTGAT CGGTCCAGCCGGACGGGATCGGCACCAGGGCGCGGGCGTCGGCGACGGCGACGGGGCCGAAGGCGCCCTCGAACAGGCCCATGACACGGTCGCCGACGGCCACCGACGTCACATCCGGAGCGACCTCCAGCACCTCGCCCGCGCCCTCGCCGCCCAGGCCCCGCCGGTCGGGCACGACGCCGAGGGTCACCATCACATCGCGGAAATTGACGCCCGCGGCGCGCACGGCGATCCGCACCTGACCCGGCGCCAACGGCTCCAGTACGTGCGCGCTCTCCTCGGGCACCAGGCTGTCCACCGTGCCCGCGCTGTCCTCGGCCACGACCAGTTGCCAGGCGGGCGCGCCGACGGGCAGGGCGATGCCGGCCGTCGCGTCCACGGCCGTCAGCCTCGGTACGAATGACCTCCCGTCGCGCACCGCCACCTGCCATTCGTCGGCGTCGAGGGCCGCGCGCGCCACTCCCGCGATGTCGGGTGCCGTGCCGGCGTCGCGTCCGGCGAGGTCGAGCAGCGTGAACCGGCCGGGGTTCTCCGACTGGGCGCTGCGGATCAGACCCCAGACGGCCGCCGCGACCGGGTCGACATCCGTCTCCTCGTCCACGGCGGCGGCGTCGCGGGTGACGATCACCAGCCGGGCCCCGTCGAGCCGCGGCTCGGCCAGCCATTCCTGCGCCGTCGCCAGGACCCGCGCCACCACCGCGTCGCCGTCCCCGCCGTCGGACTCCGGTGAGCTGGTCAGGACGACCGGTGGCACGGGTGCGCCGTCGTCAAGGGCCGCGGCGAGCGCGGCGACATCCGGGTAGCGGGTGAGCGAGGGCACGGCCTCGGTGGGTCCGCCGTCGCCGAGCACCGCCCAGTCGCCCGCCGCGGCCCTCTCCGGTCCCGCCGGGGTGGGCAGGGCGGTCCAACGCACCTCGTACAGACCGTCGTTCGGGTCCTCGCTCCGCAGCCGCGCGGTGTCGAGCGGCCGGGTGGTGATGGTGTCGGCGGTGAGGACGAGCGCGCCCGTGGTGTCGGTGAGGGTGAGCCGGTACTCGTTGCCGCCTCGGGGAGTGATGCGGGCCCGCGCGTGGGTGGCGCCGGTCGCGTGGAGGGTGATGCCGTTCCAGGTGAAGGGGAGCCAGATCTCACCGGCCGGGTTGTCGGCGGTGGCGATGAGGGGGTGGAGCGCGGCGTCGAGGAGTGCGGGGTGGACGGTGAGGGTGTCGGGGGTGTGATCGGCGGTGTCGGAGGTGGGGAGGGCGATGTCGGCGAGGAGTTCGTCGCCGTGTCGCCAGGCGGCTCGCAGCCCTTGGTAGGTGGTTCCGTAGCGATAGCCGCGCGCGGCGGCCCGCTCGTAGAAGCCGTCGATGTCGACGGGTTCGGCTCCCGGGGGCGGCCAGGCCGCCCCCGCTTCGGCGGCCGGCGGCTCGGCCGTGGGCTCGCCGGTGAGGGTGGCGGTGACGTGGTGGGTCCACTCCGCCGTGGGGTCGGACTCCGGGCGGGTGTAGACGTGGACGGTGCGGCGGTCGGTGTCGTCCGGCGGGTTGACGATGACCTGGAGCGCGAGCGGGCGGGCGGGGTCGAGCCTGAGGGGCTGCTGGAGGAGGAGCTCGTCGATGTGGGGTGTGGCGGTGTGGTCCGCGGCGTGGAGGGTGAGTTCGAGGAGCGCGGTGGCGGGCAGCAGGACGGTCTCGGCGATGGTGTGCTCGGTGGGCCATCCGGCGTGGTCGGCGGGAGGGACGTGGCCGGTGACCACCATGCCGCCGTCGGCCAGCGGCGCGGCGGTCCGCAGCAGCGGGTGGTCCAGGCTCGTGGAACCGGCCGCGGCCGGGCCGGAGGCCCCGCCGGTGAGCCAGTAGGGCCGGTGCTGGAAGGGGTAGGTGGGCAGGTCGACGGTGTCCGGGGCGGGCCGGCCGGTGAACCAGGGGCGCCAGTCGACTGGGGTGTGGGTGTGCAGCCGGGCCAGGGCGTGCGCCAGACCCTGGACGTCGGGCTGGTTACGGGTCAGCGCCGGGACGAGCAGAGGAGCGTGCGCGGGCGCGTCATCGAGCGCGTCGAGGGTGGCCTGGGCGGCGGGGATCAGTACGGCGTCCGGGCCGACCTCGAGGAACACCGCCGCGCTCGGCGCGATGTGTGCGACGGCGGGGTGGAAGCGTACGGGCCTGCGGATGTGCCGTACCCAGTAGTCCGGGGTGGCGATGTCCTCGCCCGCGGGTTCGCCGGTGAGGTTGCTGATCAGCGGGGTGTTCGGCTGGGCGAACTCCAGGTCGGCGATGGCGCGGCGGAAGTCTTCGAGAGCGGGTTCCATGAGCGGGGAGTGGAACGCGTGGCTGACGGTCAGGGCCTTGGTCCTGCGGCCGCGTTCCTTCCAGGCCGCGCCCGCCTCGGCCACCAGTTCCGCGGGGCCCGAGATGACGGTGCTGGTGGGGGCGTTGAGGGCGGCGATGGTGACCCGGCCGTCGTACGGGGCGAGGTGCTCGGTGATCTCGTCGGCGGTGGCCTCGACGGCGGTCATGGCGCCGCCGGGCGGGAGGTTGCCGAGGAGGGTGGCACGGGCGGCGACCAGACGGCAGGCGTCTTCCAGGGAGAGGACTCCGGCCACCTGCGCGGCGGTGATTTCGCCGATGGAGTGGCCGATCACGGTGTCCGGCCGTACGCCGAAGGTCTCCAGCAGCCGTGCCAGGGCCACTTGGACCGCGAAGAGTCCGGCCTGGGTGTAGGTGGTGTGGTCGAGCAGGGTGGCGTCGGGCCGGCCGTGGAGGACGACGTCCCGCAGGGGGTGGTCCAGGTGGGGGTCGAGCAGCGCGCAGACCTCGTCGAACGCGTCGGCGAAGACCGGGAAGCGCGCGTGCAGTTCGGCGCCCATGCCGGGGCGCTGACTGCCCTGCCCGGAGAACAGCCACACGGTCTGCCCCGCCGCGGGCGCGGCGTAGGGCGGGGTGATCAGGTCCGGGTGGGGACGGCCCTGGGCCAGGGCGTCGAGAGCGGCGGCCATCTCCCGGCGGTCGCCGCCGACGATCACGGCCCGCCGCTCCAACACCGCGCGCGCCGTGGCCAGCGACCGGCCCACGTCCGCCACCGGCGTGGTGTCGTCGGCTCCCAGCCGGTCGGCGAGGGAGGCGGCCAGGGCGCGCAGGGCCTGGTCGCCGCGGGCGGACAGCGGCCAGGGCACGGGGCCGGGCGGGGGAGCGTCGGTCGCGGGCCGCGGCTCGGGCAGGTCCGGGCCCTGTTCGAGGATGAGGTGGGCGTTGGTGCCGGAGGCGCCGAAGGAGGAGACGGCGGCGCGGCGCGGGCGGCCGGTCTCGGGCCAGTCGGCCGCTTCGGTGAGGAGCCTGACGTTCCCGTCGGCCCAGTCGACGTGCGGGCTGGGCTCGTCGATGTGCAGGGTGGCGGGCAGCGTACGGTGGCGCAGGGCCATCACCATCTTGATGACACCGGCGACACCGGCGGCGCCCTGCGTGTGCCCGATGTTCGACTTGATCGAGCCGAGCCGGAGCGGGTGGCCGCCGGGGCGGTCCTGGCCGTA GGTGGCCAGCAGGGCCTGCGCCTCGATCGGGTCGCCCAGCGTCGTGCCCGTGCCATGGGCCTCGACGGCGTCCACGTCCGCCGGGCCGAGTCCGGCGGTGGTGAGGGCCTGGCGGATCACCCGCTGCTGTGACGGGCCGTTGGGCGCGGTCAGCCCGTTGCTCGCGCCGTCCTGGTTGACCGCCGAACCGCGGACGACGGCCAGAATCCGGTGTCCGTTGCGCCGGGCGTCCGACAGACGCTCCAGGAGGACCAGGCCGACGCCGTCGCCGAAGCCGGTGCCGTCGGCGGCGGCCGCGAACGGCTTGCACCGTCCGTCGGGGGCCAGACCGCGCTGCCGGGAGAAGCCCGCGAACACGTCAGGGGTGGCCATGACCGTGACGCCGCCCGCGAGCGCCAGGTCGCACTCGCCGTGGCGGAGGGCCTGGGCGGCGAGGTGGATGGAGACCAGGGACGACGAGCAGGCGGTGTCCACGGTGACGGCGGGGCCCTGGAGGCCCAGGGTGTAGGCCACGCGTCCGGCGATGATGCTGCTGGTGCCGGAGAGACCGGCGTAGCCGTCCAGGCGGGGGTCGCCCGGGGGCAGTCCGGCTCCGTAGTCGTGGTACATGACGCCCGCGTAGACGCCGGTCAGGCTGTCCCGGAGGGTGTGCGGGTCGATGCCGGCGCGCTCCAGCGTCTCCCAGGCGGTCTCCAGAATGAGACGCTGCTGGGGGTCCATGGCCAGGGCCTCGCGGGGGTTGATGCCGAAGAAGGCGGCGTCGAATCCGTCGGCGTCGTAGATGAACGCGCCCTGCGAGGCGTAGCTGGTGCCGGGGTGGTCGGGGTCGGGGTGGAACAGATTGGCCAGGTCCCAGCCGCGGTTGGTGGGGAACGGGCCGAGCACATGCCGCCCGGACGCCACCAGGTCCCACAGCCCCTCGGGCGAGGTCACGTCGCCGGGGAAGCGGCACGCCATCGCCACCACCGCGACCGGCTCGTCGGCGGCGCCGGTCCCGGACCGGCCGGCACGGCGCGGCGCGGGGGAGCCGGTGGCGGTCAGGCGCTCCAGGACGTGGTCGGCCAGGGCCCGGGGGGTGGGGTAGTCGAAGACGAGGGTGGCGGGCAGGCGCAGCCCGGTGGCGGCGGCCAGGCGGTTGCGCAGCTCGACGGCGGTCAGGGAGTCGAAGCCCAGCTCCTTGAAGGCGGCCTCGGCCCGGATCGACGCGTCGGAGGAGTGGCCCAGCACCACCGAGACCAGCTGCCGCACCGTCTGGAGCAGCAGTTCGCGCCGTGCGGGCTCGTCGAGACCGGCCAGCCGGCCGGCCAGTGCGGGGCCGGTGGCGCGGTGTTCCATCGCGCGGCGCCGGGCCGGCCTGGCCAGGGCGCGCAGCATGGCGGGGCTGTCGGGGCCGAGCCTGGTGGCGTCGAGGTCGGCGGCCACCACCAGGGGGTGGGTGACATGGAGTGCCGCGTCGAGCAGCCCCAGGCCACGAGCCGTGGCGAGGGGGCGCATGCCGAGGGCGCCGAGGCGGGACAGGTCGGCCTCGGTGAGGTGCTCGGTCATGGCGGAGGTCTGCTGCCACAGGGTCCAGGCGACCGAGGTGCCGGGCAGTCCGTCGGCGCGCCGACGCGCGATCAGGGCGTCGGTCCAGGCGTTGGCCGCCGCGTATCCGGCCTGGCCGGAGTTGCCGACCGTGCCGGCGGCGGAGGAGAACACCACGAACATCCGCAGCGGCAACTCCCGGGTGAGGGTGTGCAGATGCCATGCGCCGACGGCCTTGGGCGCCCATACGCGGCCGAGTCGGCCGGTGTCCCAGGACTCCACCAGGGCGTCCTCGGTCACCCCGGCGGAGTGAACCACGCCCAGCAGCGGGTGGCTGTCGGGGATCGCCGCCAGCGCCCCGGCGAGCGCGTCGCGGTCGGCCACGTCGACCGCCGCCACCTCCGCGTGGGCGCCCAGCGCGGCGAGCCGCTCGACCAGTTCGGCCGCTTCGGGGCTCTCGGGGCCGCTGCGGGAAAGGAGCAGCACCCGCTCCACGCCATGCGCGCGCACCAGATGTTCGGCGACCGCGCCGCCCAGCACCCCCGTACCGCCCGACACCAGCACCGTGCCACCGGACAGCTCCGGGGCTCCGGCCCCTGACGTCGGAGAGGCGCGGACCAGACGTGGCGCCAGCACCCCGGCCGCGCGCACGGCGACCTGCGGCTCGTCCGCGTCGAAGGAGTGGAGTACGGCCCCGACGAGGCCCGCTTCACCGTCCGCGTCGCCATCGCCCACGTCGACGAGGGTGAACCGGCCCGGGTGCTCGGCCTGGACCGAACGGACCAGACCCCACACGGCCGCGCCACCCGGGTCCACCGCCCCCTCGTCCCTGCCCGCGGTCACGGCACCGGAAGTGACCAGGACCAGCCGGGAGTCCAGCAACCGCGGCTCGGCCAGCCACTCCTGGAGCAGAGCCAGCACGCCCCCGACGGCGGCGAGGCCGTCGTCGGGGGTGGCCGTCGCTGTCACCGGGTGGAGGACAACCGTCGGCACCGGGGTCCCGTCGTCCACCGCGGCGAGCAGCGCGCCGAGGCCGGCGTGTTCCGCCACCCCGGCGAGCGGTGCGCCACCATTGCCCACCGCCGCCCAATCGGCGTCCGGCGGGGCCGAATCGTCGCCGGTCCCGCCCCGCGCGGGCACCCAGTCCACGACGAACAGGCCCTCGGCCGCGCGCTTCCCAAGGGCCCGCAGCTCGGCGACGCCGGTGGGGCGCGCGGCGACCGACGCGGCGTCGAGGACCGGAACGCCCGTCTCGTCGGTCAGGGAGATCCGCCGCCCGTTGCCCTCCGGGGAGATACGGACCCGTACGCGGCGCGCCCCCGTGGCCCACAGCGACACACCGCTCCAGGCGAACGGCAGCCACACCTGTCCGGTGTCCGAGGCGTCCTGACGGCCGCCGTCGAGGAGCGTCGGGTGCAGGGCGGCGTCCAGCAGCGCCGGGTGGACGCCGAACCGGTCGGCGCCCTCACCGGCCGCCTCGGGCAGCGCGACCTCGGCGAGCAGATCGGCGCCGTCCCGCCACGCCGCGGTCAGGCCGCGGAACGCGGGCCCGTAGCCGTAGCCGCCGGCCACGGCCTGTTCGTAGAAGTCCGCGACGTCCAGGGGTTCCGCGCCGGGCGGCGGCCAGGGAGCCGCCACGGCGTCCGACGGGGCCTCACCGCCCTCGGGCGCGAGGACGCCCATGGCGTGGCACACCCACCCGTCGGGGGCCTCCGTGTCGAGCTCGTCGGGGCGGGAGTGCACATCCACCGTCCGCCTGCCCTCGTCGTCGGCGGGCCCCACCGCGACACGTACGCGCAGCGCCCCCGAGGCGGGCAGGACGAACGGGACCTGGAGGGCCAGCTCCTCCACCGCGGCGCAGCCCGCCTCGTGGGCGGCCTGAAGCGCCCACTCGACCATGGCCGCCCCGGGCAGCAGCGCGGTGTCGCCGACGATGTGGTCGGCGAGCCAGCCCCCGCCCGCGGCGGCCGAGAGGCGTCCGG TCAGCACCATGCCGCCGTCGGGCAGGCTCACCGCGGCGGCCAGCAGCGGATGGTCGACGGCGCGCAGCCCGGCGGCGGACACATCCCCTGTGCCCTTGCCGTCGGCGGGTATCCAGTACCGCCGCCCCTGGAAGGGGTAGGTGGGCAGCTCGACGGCGCGGGGCGCGGGCCCGGCCGGGAACCACGGGCGCCAGTCGACCTCGAGACCCGCGGTGTGGGCATCGGCGAGCGCGAGGGCGAGCTGGTGTGCGCCGCCGTGGTCGCGGCGCAGCGTGGCGAGAGAGGCGGCGGGGGTGCCGGACTCCTCGAAGATGTCCTGGAGGCTCGTGGACAGGATGGGGTGCGGGTTGCACTGGATGAAGGCCCGGTATCCGTCGCCCAGCAGACGGCCCACCGCGTCGGCGAAACGGACCGGCCGGCGGAGGTTCTCGAACCAGTACTCGGCGTCCAACCGCGCGGTGTTCAGCCGCTCCGCGGTGACGGTGGAGTAGAACGCGATCTCGGACTCGGCCGGTGTCACCTCGCGCAGGGCGGACACCACATCCTCGTGGATGCGGTCCACATGAGGGCCGTGGGAGGCGTATTCGACGTCGATGGCGCGAGTGCGCGCGCCACGCTCCCGCGCCGTGTCCAGGACGGCGGCCACCCGGTCCGCGGACCCGGAGACCACCGTGGCGTGCGGGCCGTTGACGGCGGCGACGGCCACGTCCCCGGCGTCGGGGCCCAGCTCGGCGAGGAGCCCGGCGGTGTCCTCGGCGCCGAGGGAGACCGAGGCCATGGCGCCGTGTCCGATCAGGGGACGGAGAGCCTGTACGCGCAGGGCGATGACCTTGGCGGCGTCGTCGAGGGAGAGGGCCCCGGCCACGCACGCCGCGGCCACCTCGCCCTGGGAGTGCCCGACGACGGCCGCCGGGACGATCCCGAAGCTCCGCCACAGGGCGGCCAGCGAGACGTGGATGGCCCACAGCACCGGCTGCACCACATCGTCCCGGCCGGCGTCCGTCGCCCGGTCCGTGCCGCGCAGCACGGCGGTCAGCGACCAGTCCACATACGGCGCCAGAGCCCGCTCGCACTCGGCGATCCGGGCGGCGAAGACCGGCGAGGAGTCCAGGAGTTCGCGCGCCATGCCCGGCCACTGGGCGCCCTGGCCGGGAAACACGAACACCGGGCCCGCGCCGACCGGGCCCGCCGTACCGGTGACGACCGCCGGATCCGGCTCGTCGGCGGCCAGCTTCGCCAGCCCGCCCAGCAGCGCCGCACGGTCCGCGCCCACCACCACGGCCCGGTGCTCGAACCGGGCCCGCGCCGTCGCCAGAGACCACCCCACCTCCGCCGCACCGGCGGCCGGATCGCGCTCCGCCGCCTCGGCCAGACGCCGCGCCTGTTGCGCCAGCGCCGCCTCGTCCCGCGCGGACAGCAACCACGGCACCACGCCGCCGTTCACCACGGCCCGCGGCGCCACCTCCGCCTCTCCCGTGCCTTCCGGGTCGCTTTCGCCCTGGACCGCCTCGGCGGGTTCGGGCGGCTGCTCGATGATCAGGTGGGCGTTGGTCCCGGAGACGCCGAACGACGACACCCCGGCCCGGCGCGGGTGGTCGGTCTCCGGCCAGTCCACCGGCTCGGTCACCAGACGGATGGCGCCCGATCCCCAGTCCACGTGCGGCGTCGGCTCGTCGATGTGCAACGTCGCGGGGATCACCCCGTGCCGCATCGCCATCACCATCTTGATCACGCCCGCCGTCCCGGCGGCCGCCTGGGTGTGACCGAGGTTCGACTTGACCGAGCCCAGCAGCAGCGGCCGGTCCGCCGGGCGCTGGTGGCCGTACGTGGCGTGCAGCGCGTCGGCCTCGATCGGGTCGCCGAGCCGGGTGCCCGTGCCATGCGCCTCCACCGCGTCCACCTCGGCGGACGACAGGCGGGCGTTGACGAGCGCCTGACGGATGACCCGCTGCTGGGAGGGCCCGTTGGGCGCGGTGATGCCATTGCTCGCGCCGTCCTGGTTGACGGCCGAACCCCGGATGACGGCCAGCAGCTGGTGTCCGTTTCGGCGGGCGTCGGAGAGCCGTTCGAGCAGGACCAGCCCGACGCCCTCGCCCCAGCCCGTGCCGTCGGCGGCGGCCGCGAACGGCTTGCAACGCCCGTTCGGCGCCAGGCCGCGCTGACGGGCGAAGCCGGTGAACACCGCGGGCGAGGCCATCAGCGTCACACCACCGGCCACCGCCAGCCGGCACTCGCCCCGCCGCAGTGCGTGCATCCCCAGATGCATGGCCACCAGCGACGCCGAGCAGGCCGTCTCCACCGTCAGCGCGGGACCCTCCAGGCCGAAGGCGTACGACAAACGGCCCGACAGCATGCTGGGCGCGACGCCCGCGTACCCCTCGGTCTCCTTGCGGACGGGAACGCCCGACGTGGCGGAACCGATGGTCGCCGCGCCCACGAAGACACCGGTCTGGCTGCCCCGAAGGCTCGTCGCGTCGAGACCGGCGCGCTCGAACAGCTCCCAGGCCACTTCGAGCAGCTTGCGCTGCTGCGGGTCCATGGCCAGTGCCTCGCGCGGGCTGATCTCGAAGAAGCCCGCGTCGAACGTGTCGGCGTCGCGCAGGAACCCGCCCTCGCGCGTGTACGTCGTGCCGGGGTGGTCGGGGTCCGGGTGATACAGACCGTCCAGGTCCCAGCCGCGGTCCGTGGGGAACGGCCCGACGGCGTCCACCCCCGAGGCCACCAGGTCCCACAACGCCTCGGGCGAATCCACCCCGCCGGCGAACCGGCACGTCATCGCGGTGATGGCGATGGGCTCCTGGTGCCGCTCCTCCACCTCGCGCAGGCGCCGCCGCGTGTCGTGCAGGTCCGTCGCCACACGCTTGAGGTAGTCGACAAGCCTGTCTTCATCCGGCATGTGAAGTCACGACCTTCATACGTGTCGGGGCGGGCAACTGAGCTGTCACGAGGTGCCGAGCTCGTTGTCGATGAACGCGAGCACCTCGTCGGCGGTGGCGAGCGTGAGCCGGTCGGCCGCGCTGTCCTCGGCCGGCGGCGCGGACGCCGCCTTCCATTGGGCCAGCAGACCCTCAAGCCGATCTGTCACCGCCCGCGCGTCCGCCTCGGGCAGCTCGAGCGCCGACAGCGTGGACTCCAGCTTCGCCAGCTCGCCCAGCACGGGGTCCACCCCGCCCCGGGCCGCCGACCCCCCGTCCGGCACGAGCAGGCCGCGCAGGTGGTCGGCCAGCGCCCTGGCCGAGGGGAAGTCGAAGACCAGCGCGGCCGGCAGCCGGAGCCCGGTCGCCGCCGAGAGCCGGTTCCGCAGCTCGACGCCGGTCAGCGAGTCGAAGCCCAGATCCGTGAACGGCGCGTCCTCCCGTACGCCTTCGGCGTCGGCGTGGCCGAGCACGGTCGCGGCATGGCCGCGCACCAGCGTGAGCAGCTGCTGCCGCTGCTCGGCCTCCGGGAGCCCGGCGAGCCGGCGCGTCCAGTCGTCGGGGCGCTGGGTGGCCGCGGCGGCGCGCCGCGTGGCCCCGCTCG GGCGGGCGGTCAGGGCCCGTAGCGCGGGCGGCAGACCGGCGATCGGCTGCGCGGCCAGCGCGCGTACGTCGAGGTTGAGCGCCACGAGCTGCGGGTCGCCGTGTCGGTGTGCCGCGTCGAAGAGCGCCAGCGCGTGGTCGGTGGCCAGCGCCGTGACGCCGGTGCGGCCGATCCGCGCCACATCGGCCTCGCTGAGCTGCCCGGTGATATCGCTGGTGGCGGCCCACAGGCCCCAGGCGATGGAGGCGGCGGGCAGACCGGCGTGCTGCCGCCGGGAGGCGAGGGCGTCGCAGTAGGCGTTGGCGGCGGCGTAGTTGGCCTGCCCCGGGTTGCCGAGGGTGCCCGAGACGGATGAGAAGAGGGCGAACATGGCGAGGGGGAGGTGCTCGGTGGCCCGGTGGAGGTTGGCGGCGCCGGTCGCCTTGGCGGCCCAGACACGGTGGAGCGTGTCGGGGGTCTGGGAGGTGATCATCGCGTCGTCCACGGTGCCGGCGGTGTGGATGACGCCGGTGAGGGGGTGGGCGGGGTCGATGGTGTCGATGATCTGCCGCAGGGCGGGGAGGTCCGAGGTGTCGGTGGCGGTGATGTGGACGGTGGCGCCGAGGGCGGTGAGTTTGTCGGTGAGTTGTTGGGCGCCGGGGGCATCAGGGCCGCGTCGGCTGAGGAGGTGGAGGTGGGTGATGTGGTGGGTGGTGACGAGGTGTTCGGCGATGAGGGCGCCGAGGGTGCCGGTGCCGCCGGTGATCAGGACGGTGCCGTTGGGGTCGAGGGCCGGCGGGATGGTCAGGACGACTTTGCCGGTGTGCTTGGCCTGGCTCATGTGGCGCAGCGCCTGACGCGCCTTGGGCAGCGGCCACGTCTGGACCGGGGGCGGGGTCAGGGCCCCCGCGTCGAACAGGGCCACCAGGGTCGCCAGCATCCGCGCGACGCGCTCCGGCCCGGCGTCCGAGACCAGGTCGTAGACGCGGTAGTCCACCCCGGCGAAGTCCTTGGCCACCTGCTCGGGGTCGCGGAGGTCGGTCTTGCCCATCTCCAGGAAGCGGCCGCCGTCGGCCAGCAGGCGCAGGGAGGCGTCGATGAAGTCACCGGCGAGGCTGTTGAGGACCACGTCGACCTTCGGGAAGGCGTTCTCGAAGTCGAGGTCGCGGGAGGAGGCGCGGTGGGTCTCGTCGATGCCCATGGCCTCCAGGACATGGTGTTTGCCGGGGCTGGCCGTGGCGTACACCTCGGCGCCCAGGTGGCGGGCGATCTGCACGGCGGCCATGCCCACGCCGCCCGCCGCCGCGTGGACGAGGACCGACTCGCCCGCCCTCAGGCGGCCGAGCTCCACCAGGCCGTACCAGGCGGTCAGAAACGCCACGGGGACGGCCGCCGCCCGGGAGAAGTCCCAGGCCTCCGGCATCCGGGTCACCATCCGCGCGTCGGCCACGGTGACCGGGGCGAACGCGCCGTCGAAGACCCCCAGGACGCGATCGCCCGGGACCACCGTGGTGACGTCCGGGGCCACTTCGAGGACGACGCCCGCGCCCTCGCCGCCGATGCGGGTCTGGTCGGGCACCATGCCGAGGCTGATCAGGACATCGCGGAAGTTGACACCGGCCGCCCGCACCTCGATGCGCACACGTCCGGGCGCCAGCTCCTCCTCCGCCTCGGGGAAGGGCACCGGCGCCACGGAGTCGAGCGTCGCGGTGCCCGTGGTGCCTGCGGCGTCCAGCCGCCAGGCCCCCTGGCCGACGGGGGGTACGAGCGTGGCCGAGGCGGTGGCGCGGACGAGCCGGGGCACCAGAATCCGGCCGCCGCGGAGCGCCACCTGCGGTTCGTCGAGGTCGACGGCGCAGCGGACCGCGTCGGCCACCTGGCTGTCCGACTCCGGATCCGTGTCTGTGTCCGTGTCCAGGAGGACGAAGCGGCCGGGGTTCTCCGTCTGGGCGCTGCGGACCAGACCCCAGACGGCCGCCGCGACGGGGTCGACGTCCGTCTCCTCGTCCACGGCGACGGCGTTACGGGTCACCATCACCAGACGGGCGTCGTCGGGGCGCGGCTCGGCCAGCCACTCCTGTAGCTGCGTCAACGCCGCCTGTATGGGCTCCAGTTCACCGTCGCCCTCCGCGACCGGTGCCTCGGCGAAGACCGCCCGCGAGGCCGGCGTGGCCTCGTCGGCCAGGGGCGAGGCGGGCGTCCACTCCAGGGTGAACAGGCCTTCGGACGAGGGCTTTTGCGGAGCGTCGAGGGCCATGGGCCGGGTGGTGACGGCCTCGGCGGTGAGGATGAGTCGGCCGGTGGCGTCGGTGAGGGTCAGGCTGTGCTCGCTGTCGCGGGAGGTGATGCGGACGCGGGCGTGGGTGGCGCCGGTGGCGTGGAGGGTGACGCCGTTCCAGGAGAACGGCAGCCAGACCTTGCCGTCCGAGGTGTCCGTGACGGCGACGAGGGGATGGAGCGCGCTGTCGAGGAAGGCGGGGTGGACGGCGAGGGCTTCGGCGGGGTGGCCCGTGGCGTCCGTGGCGTTTGCGGTGGGCAGGGCGATCTCGGCGAGGAGGTCGTCGCCATGGCGCCACAGCGTCTGAAGCCCCTGGAAGGCGGGTCCGTAGTCGTAGCCGTCCGCCGCGGCGTGGTCGTAGAAGCCGGTGATGTCGACGGGCTCGGCGCCCGGGGGCGGCCAGGCCGTCTCAGCTTCGGGCGCCGCCCGGTCGACCGCGGGTTCGCCGGAGAGCGTGGCGGTGGCGTGCCGGGTCCACTCGGCCGCCGGGCCGGACTGCGGGCGGGTGTAGACGGCGAGGCTCCGGCGGTCGTCGTCGTCGGCGCCGACGACGACCTGGAGGTCGAGCGGGCCCGTGGGGTGGAGGACGAGCGGGTGCTGGAGGAGAAGCTCCTCGACGTGCGGGCTGTCGGTGTGCTCGGCCGCGCTGAGGGCGAGTTCGAGGAGTGCGGTGCCGGGCAGCAGCACGGTCCCGGCGATGGTGTGTTCGGTGAGCCAGCCGTCGTGGTCCGCCGCCGGGATCCGGCCGCCCAGAACTAGTCCGCCGTCCGCCAGCGGCGCCGCCGCGTCCAGCAGCGGATGGTCCAGGCCGGTGGCGGCGACGGAGGAGATGCGGGGAGCGATCCAGTACGGCCTGCGCTGGAAGCGGTAGGTGGGCAGCTCGACCACTCGGGGGGCCGGGTCGGTGGGATACCAGGGCGTCCAGTCCAGCGGGGCCCGGGTGGTGTGCAGGGACGCCAGGCCGTGGGCCAGCGCCCGGACGTCGGACTGCTTGCCGGTGAGCGTGGCGAGCGCCGTGGCGGTGGTCTCACCGGGGAGGGTGTGGTGGACGGCGTGGGTCAGCACCGGGGCCGGGCCCAGCTCCAGGAAGACGGCCGTGTCCGCGGCGAGATGGGTGATGGTCTGGTGGAAGCGGACGGGGCGGCGGATGTGCCGGGCCCAGTAGTCGGGGGTGGCGATGTCCTCGTCCGCGGGTTCGCCGGTCAGGTTGCTGATGAGGGGGATACGGGGCAGGCGGAAGTCCAGGTGGC CGATGGCCTCGGCGAACGGGGTGAGGATGGGCTCCATCTGGGGGGAGTGGAAGGCGTGGGAGACCGGGAGGGAGCGGGTCCTGCGGCCCCGGTCCCGCCAGACGGCGGTGACGGTGGCGACCTGGTCGGCGGGTCCGGAGATCACCGTGTGGCCGGGGGTGTTCATCGCCGCGATGGCCAGCTCCGGGAAGTCGGCCAGCGTGGCGGCGACCTCCTCCTCGTCGGCGTTGACGGTGGCCATGGCGCCGCCGGCCGGCAGGTTGCCCATGAGCGTGGCGCGGGTGGCGACGAGGTGGGCGGCGTCCTCCAGGGAGAGGACGCCCGCGAGGTGGGCGGCGGAGATCTCGCCGATGGAGTGTCCGGCGACGGCGTCGGGGGCCAGGCCCATGGCCGTCAGAAGGCGGGCGAGGGCGATGTGGAGGGCGAACAGCCCGGCCTGGGTGTAGGTGGTGTGGTGGATCAGGTCGCCGAGTTCGGGGTCGGTGGCGAAGACCACGTCGCGCAGCGGGCGGTCGAGGTGGGGGTCCAGGAGCGCGCACACCTCGTCGAAGGCCCCGGCGAAGACGGGGAATCGCCGGTGGAGCTCGGCGCCCATGCCGGGGCGCTGGCTGCCCTGCCCGGAGAACAGCCACACCGTCTTGCCCGGGGTGTCCGGGGCTGCGCCGCCGGGGGTGGCGAGGCCGGGGTGGGGCTCCCCGCCGGCCAGCGCCTCCAGGGCGGCGGTCAGCTCCTCGCGGCCGTCCCCGACGATCACGGCCCGCCGCTCGAACACCGCCCGTGTCGTGGCCAGGGACCAGCCCACCTCGCCGACCGGGAGATCGGGGCGCGCGGCGAGGTGTTCGGCGAGCGCCGCGGCCTGTTCGCGCAGGGCCCGGTCGTCGCGGGCCGACAGCGGCCAGGGCACGGGGCCGGGCAGGGGAGTGTCGCTCGCCGGCCGGGGTTCGGGGAGGTCGGGGGCCTGTTCGAGGATGAGGTGGGCGTTGGTGCCGGAGGCGCCGAAGGAGGAGACGGCGGCCCGGCGCGGGCGGCCGGTGTCGGGCCAGCCGACGGGCTCGGTGAGGAGCCGGACGTTTCCGTCGGCCCAGTCGATGTGCGGGCTGGGCTCGTCGATGTGCAGGCTGGCGGGCAGTTGGCCGTGGCGCAGGGCGGAGATCATCTTGATGACTCCGGCGATGCCGGAGGCCGCCTGGGTGTGGCCGATGTTGGACTTGACCGAGCCGAGCCACAGCGGCCGGTCGTCGGGGCGGTCCTGGCCGTAGGTGGCCAGGATGGCCTGTGCCTCGATGGGGTCGCCCAGCGTGGTGCCCGTGCCGTGGGCCTCGACGGCGTCGATGTCGGCGGTGGTCAGGCCGGCTCCGGCGAGGGCCTGGGCGATGACGCGCTGCTGGGAGGGGCCGTTGGGGGCGGTCAGCCCGTTCGACGCGCCGTCCTGGTTGATCGCCGAGCCGCGCACCACCGCGAGCACCCGGTGGCCGTTGCGGCGCGCGTCGGAGAGGCGTTCCAGCAGGACGAGCCCGGCCCCCTCGCTCAGGCTGGTGCCGTCGGCCGCGGCGGCGAACGGCTTGCACCGTCCGTCGGGCGCCAGGCCGCGCTGCCGGGAGAAGCCGGTGAACGGATCGGGGGTGGCCATGACGGTCACGCCGCCCGCGAGCGCCAGATCGCACTCGCCCTGCCGCAGCGCCTGGGCGGCCAGGTGCATGGACACCAGGGACGACGAGCACGCGGTGTCCACGGTGACGGCGGGGCCCTGAAGCCCCAGGGTGTAGGCCACGCGGCCGGAGATGATGCTGCCGATGGTGGAGAGCATCGTGTAGCCGTCGAGACGCGGGTCGCCCGCGGGCAGGCCGGTGGCGTAGTCGTGGTAGATGACGCCCGTGTAGACGCCGGTCAGGCTGTCCTTGAGGGTGTGCGGGTCGATCCCGGCGCGCTCCAGGGCCTCCCAGGCGGTCTCCAGGACGAGCCGCTGCTGCGGGTCCATCGCCAGGGCCTCACGGGGGTTGATGCCGAAGAAGGCCGCGTCGAAGCCCTCGACGTCGTAGATGAACGCGCCCTCGGAGGCGTAGCTGGTGCCGGGGTGGTCGGAGTCGGGGTGGAACAGATTGGCCAGATCCCAGCCGCGGTTGGTGGGGAACGGCCCGACCACATGCTCCCCCGCCGAGACCAGGTCCCACAGCCCCTCGGGCGAGGTCACGCCACCAGGGAAGCGGCACGCCATCGCCACCACCGCGATGGGGTCGTCGTCCGCGGCGCGAGCCGACCGGCCCCGCCCGATGAGGGGCGAGGCGGTGGCGGACGCGGTGGCGGTCAGCCGCTCCAGGAGATGGCCGGCGAGGGTCTGGGGGGTGGGGTAGTCGAAGACGAGGGTGGAGGGCAGGCGCAGCCCGGTGGCATCGGACAGCCGGTTGCGCAGCCCGACGGCCATGAGGGAGTCGAAGCCCAGCTCCTTGAAGGCGGCCTCGGGCCTGATGGACGTGTCGGAGGAGTGGCCCAGGACCACGGCGGCCATCTGACGCACCATCTCCAGCAGCAGATCGCGCTGTCCCGAGGCGTCGAGACCGGCGAGGCGTGCGGCCAGGGCGGGGGACGCCGACTGGCGTTCCGCGGCGCGGCGCCGGCCGGACCGAACGGGCGCGCGCAGCAGCGCGGGGACCACGGTGGCCGAGATGGCCGCGGCGGTCGACCGGGCGGTGTCGAGGTCGGCGGCCACGACCGCGGGGTGTGGGAGGGAGAACGCCGCGTCGAGCAGGCGCGGGGCGTGGGACGCGGCCCGCGCCTCCAGGGCCCAGGCCACGCTCAGGCCGGGGAGCCCGGCGGCTCGCCGATGGGAGATCAGGGCGTCGGCGCGGGCGTTGGCGGCGGCGTGACCGGCCCGGCCGGGGCCGCCTACGAGGTCGGCGGCCGAGGAGACCACCACGAACAGCCGCAGCGGCAGATCGCGGGTGAGGGTGTGCAGATGCCACGCCTCGGTGGCGGGCGCCCAGGCCCGCGTCAGCCGCTCGTCGTCCCAGGACTCCACCGGCGGCGCGCCGTCGGCCGCCCCGGCGAGATGCACCACGCCCACCAGCGGACGGCTTTCGGGTACGGAGGCCAGAACCTCCGCCAGCGCGGAGCGGTCGGCCACATCGACGGCGGCGGTCTTCGCCTCCGCCCCCAGCGCCGTCAACCGCTCGGCCGGCGCGGCCGCTTCGGGATCCTGGCCGCCCCCGGGGGAGAGGAGCAGCACACGCGCCACGCCGTACGCCCGCACCAGGTGTTCGGCGACCGCGCCGCCCAGCGGGTCCGTCCCGCCCGACACCACCACCGTGCCACCGGACAGATCGACCTCGGCGGCCGCGTCGGTCGGTTCGACGGCGCGCCGCAGACGGGGGATCGACACCGCGCCCGAACGGACCGCGATCTGCGGCTCGTCGGCGTCGAGCGACCGGCGGACCGCCTCGGCCCACTTCCCGTCCCCGTCCGCGTCGGCATCGACCAGGGTGAACCGGCCCGGGTGTTCGTTCTGGACT GCCCGGACCAGGCCCCAGACGGCCGCGCCTGCCGGGTCCACCGGCCCCTCGCCGTCGCCGACCGCTACCGCGCCGGAGGTGATCAGCACCAGCCGGGAGTCCCGCAACTGAGGTTCCGCAAGCCAGCCCTGGACAAGGCCCAGCGTGTGCCGGACCGCGGACAGGTCCGCGTCGGGGTCGGGATTCGGGGCCACGGTGGCGAGGACGACGGGCGGCGCGGGCTCTCCGCCCTCCACCGCGGCCAGCAGCGCGGCCAGGTCGGCGTACCGGCGCCCGCCGAAGGGGCGCGCCGCGGCAGGAAGGGCGTCGAGCGTGGCCCAGCCGGCCTCGGCCTCCTCCGGCTCGGGACGGTCCTCCGACGGCGGCAGCGGCGTCCACTCCACCGTGAACAGGCCGCCCGCGGCGTGTCGTTCGGCGGCTTTCAGCCAGTCGCCGTCGGCGGGACGCAGCGCGACCGTATCCACGTCCAGCACCGGGGCGCCGGTCGCGTCCGCGACGACGACGCCCAACTCGCGGCCCCCGCTGTCCTCCCGCCCCAGCGACCGGATCCGGACCCGGACACTCCGTGCGCCGCCCGCGTGCAGGGACACACCGCGCCAGGCGAACGGCAGCCACCGCGGGCCGTCGGAGTGAAGGTCGTGCAGCGCCACCGGGTGCAGGGCGGCTTCGAGCAGCGCGGGATGGATGCCGAACTCCTCGCCCCTCTCCTCGGCCCCGTCGGGCAGGGTCACCTCGGCGAAGAGATCGGAGCCGTGCCGCCACACCGCCTGGACGCCCTGGAACGCGGGGCCGAACCGATGACCTTCGGCCTCGGCGCGCGCGTACAGCTCGGCGATGTCGGCGGTGTCCACGGGCTCGGCCCCGGCCGGGAGCCAGGCGCCGGTCAGCTCCGTCCCGTTCGTTGTTTCGCGGGCGAGGGTGCCCGTCGCATGGCAGGTCCAGGCGTCCCGCCCCTCGGGCCCCTCCGGTGTGCCGGACGCGGGCTGGGAACAGACGCGTATACCGCGCCGCCCATCCCCGTCGGGCGCGTCAACCACGACCTGGACGCGCAGTTCAAAGGCGGGGGAGAGGACCAGGGGGACCTGGAGGGTCAGCTCCTCGACCGCGCCGCAGCCCGCCTCGTCGGCCGCCCGCAGGGCCCACTCCACGAGCGCCGTGCCGGGCAGCAGGACGGTGTCGCCGACCGTCTGCTCACCGAGCCGGCCCGCGTCACCGGCCGCCGGAAGCCGCCCGGTGAGCAGCAGCCCGCCGTCGGCGAGGCCCGTCGCGGCCGACAGCTGAGGGTGTGCCACCGGCCGCAGTCCGGCGGCGCTGACGTCGCCTGCCGGGCGGCCGGCCGCCATCCAGTGGCGCTGCCGCTGGAAGGGGTAGGTGGGCAGGTCGGCTGTGGTGGACGGGGTGACGGGATACCAGGGTCGCCAGTTGACGGCCAGGCCGGTGGTGTGGGCGTGGGCGAGGGCGTCGGCGAGGTCGTGGGGGGTGCCGTGGTCGCGGCGGAGGGTGGGGATGGTGGTGGCGGGGATGTCGGCGTCGTCGATGGTGTCCTGGATGCCGGGGGTGAGGACGGGGTGCGGGCTCACTTCCACGAAGTGGCGGTGGCGGTGGGCCAGGAGGGCGGTGAGGGTGTCGGCGAAGCGGACGGGGTGGCGGAGGTTGGTGAACCAGTACTCGGCGTCCAGCGGGGTGTCCGCCAGTGGCTCGGTGGTGACGGTGGAGTAGAAGGGGATGGTGGGTGTGGCGGGGGCGAGGCCGGAGAGCCGGTCGAGGATGGTGTCGCGGATGCGGTCGACGTGGGGGCTGTGGGAGGCGTAGTCGACGTCGATGGTGCGGGTTCGCGCGCCTTGGGTCTCGGCGGCGGTCAGGACGGTGGCGATCTGGTCGGGGGGTCCGGAGATGACGGTGGAGCGGGGGCCGTTGTGGGCGGCGATGGCGATGCCTTCGGCAGTCTCGCCCAGGTCGGCCAGGATGTGTGCGGTGTGGTCGGCGGTGAGGGTGAGGGAGGCCATGGCGCCGTGTCCGGCGAGGGCGCGGAGGGCCTGGCTGCGCAGGGCCACGACTTTGGCGGCGTCCTCAAGCGTAAGGGCTCCGGCCACGCAGGCGGCGGCGATCTCACCCTGGGAGTGGCCGATGACGGCGGCCGGAGTGACACCGAAGTCCTGCCACATACCGGCCAGAGACACCATCATGGCCCAGAGGGTCGGCTGGACGACATCGACGCGCTGGGGGTCGGTGGTGTCCGTGCCGCGCAGCACGGCGGTGAGTGACCAGTCGACGTACGGGGCCAAGGCCTTTTCACAGGCGGCGATCCTGCTGGCGAAGGCGGGGGAGGAGTCCAGGAGTCCGACGGCCATGCCGGCCCATTGGGAGCCCTGACCAGGGAAGACGAACACCGGGCCGTCCTGGATGTCCAGGGCGGCCCCGCGCACGAGGTGGGGGTGTCCGCCGCCGGTGGCCAGGGCCTCCAGCGCGGCGGTGAGCCCCGGCAGGTCGGTTCCGGTGACCACGGCGCGGTGGTCGAAGGTGGCGCGGGTGGTGGCCAGCGACCAGGCCACCTCGGTCGGTGAGATACCCGGAGTGTCCGCGATGGCCCCCGCCAGTCGGCGCGCCTGCTCGGCCAGGGCCTCGTGGGTACGGGCCGAGAGCACCCAAGGCAACACGCCGCCCACGACGACCGGTTCGGCCGCATCCGTGTCGGGTTCCGGTGCCTCAGGGGCCTCTTCCAGGATGACGTGGGCATTGGTGCCGGAGATGCCGAATGCCGAGACGCCTGCCCGGCGCGGGCGTTCCGCGGTCGGCCAGTCGACCGGCTCGGTGAGCAGGCGCACCGCGCCGCTGCTCCAGTCCACATGCGAGGTGGGCTCGTCGACGTGGAGGGTGGCGGGCAGGGTGCCGTGGCGCAGTGCCATGACCATCTTGATGACACCCGCGATCCCGGCGGCCCCCTGGGTGTGGCCGATGTTCGACTTGATCGAGCCCAGCCACAGCGGGCGGTCCTCCGGCCGCTCCTGGCCATAGGTGGCCAGCAGTGCCTCCGCCTCGATCGGGTCGCCGAGCGTGGTGCCCGTGCCGTGCGCCTCCACCGCGTCCACCTCGACCGCGGCCAGCCCGGCGTTGGCCAGGGCCTGGCGGATCACCCGCTGCTGCGAGGGCCCGTTGGGGGCGCTCAGCCCGTTGCTGGCGCCGTCCTGGTTGACGGCCGAGCCCCGGATGACGGCGAGCACGTGGTGTCCGTTGCGGCGGGCGTCGGAGAGCCGTTCCAGCAGGACCAGGCCCGCGCCTTCGGAGAAGCCCGTGCCGTCGGCCGCCGTGGCGAACGGCTTGCACCGCCCGTCCGGGGCCAGGCCGCGCTGCCGCGAGAACTCCGTGATCACGTTCGGGGTCGACATGACCGTCACACCGCCGGCCAGCGCCAGATCGCACTCGCCCTGCCGTAGCGCCTGGACCGCCAGATGCATCGCCACCAGCGACGACGAGCACGCCGTGTCCAGCGTGACCGCCGGACCCTCCAGG CCCAGGGTGAAGGCGATCCGGCCGGACACCACGCTGAGCGCGTTGCCCGTCAGCAGAAAGCCCTCCGCGCTCTTCTCGATGTGCGGGGTGCCGAAGCCGGGCACGCCGCAGCCCGTGTAGACGCCGGTGGAAGAGCCCTTGAGGGCGGTGGGGTCGATATGGGCGCGTTCGAGGAGTTCCCAGGCGGTTTCCAGGAGGAGCCGCTGCTGGGGGTCCATGGCGAGGGCTTCACGCGGGCTGATGTTGAAGAAGGAGGCGTCGAATTCATCCGCGTCGTGGACGAAGCCGCCCTCCTGGATGTAGCTGGTGCCCGGGTGGTCGGGGTCGGGGTGGTAGAGGGCGGCGAGGTCCCAGCCGCGGTTGGTGGGAAAGGGGCCGATGGCGTCGGCGCGGGTGTCGACCAGGTCCCACAGGGCCTCCGGGGATGCGATGCCACCGGGGTAGCGGCAGGCCATGGAGGTGATGGCGATGGGCTCGTGGTGGCGTTGCTCCGCTTCGCGCAGGCGTTGTCTGGCCTCGCCCAGGTCGAGGGTGACCCGCTTGAGGTACTGGCGCAGCTTGTCTTCCGTGGACGGCATTGGTCCTCCCCCACTCCTCGTCAGGACGAGCCCAGTTGCTGGTCGATGAGCGCGAACATCTCGTCGTCCGAGGCCGACTCCAGCGCCTCGCCGTCGATGTCCTTGGCCCCGGCCGTGCCGCCCTCCAGCAGGGACAGCAGAGCGTGGAGCCGGCCCGCGACCGTCTCGCGGGCCTTCCCGTCCATGGCGTGGCCGGTCAAGGTGGACTCGAGCCTTGCCAAGTCGCCCAGCACGGAACCGAGACGGTCCACCGCCGCGCCGGGCGTCCGGTCCGCGGGGGCGAGCTTCTGGTCCAGATAGCGGGCCATGGTCTCCGCGTCGGGATAGTCGAAGACGAGGGCGGCGGGCAGCCGCAGCCCGGTCGCGGCCGAGAGCCGGTTGCGCAGCTCGACGGCGGTCAGCGAGTCGAACCCCAGCTCCTTGAACGAGGTGTCCGCCCGCACGGCAGCCGGGTCGCTGTGGCCCAGCACCGTCGCCGCGTGCGTACGCACCAGGTTCACCAGCTCGCGGTGGCGCTCCGCGTCGGAGAGCGCCGCCAGACGGCCCGCCCAGTCTGTGGACCGGGCGGCGGCCGCGGCGCGCCGTACGGGCGCTCCGGCCGCCGCGATCGTACGGAGCAGGGCGGGCACGGCGTCGGACGGCTGGGCGCCGAGCGTACGCGGATCGAGGTCGAGCGCCAGCGGAGCCGCGCCGCCGTGTCGCAGGGCGGCGTCGAACAGCGCCAGCGCCATCGGGGTGGACAGCGGGGTGAAGCCGGTGCGGCTCATCCGCGCGAGGTCGGCCTCGGTGAGCCGGCCCGTCATATCGCTGGTGGCGGCCCACAGGCCCCAGGCGATGGACACGCCGGTGAGACCGGCGTGGCGCCGCCGGGTGGCGAGGGCGTCGCAGTAGGCGTTGGCGGCGGCGTAGTTGGCCTGCCCGGCGGTGCCGAGGGTGCCTGCGGCGGAGGAGAAGACGACGAACATGGCGAGGGGGAGGTGCTCGGTGGCCCGGTGCAGATGAGCCGCGGACGTCGCCTTCGCCGCCCAGACCGGCGCCAGCCGCTCCGCGGTCTGCGCGGTCAGCACCGCGTCGTCCAGAGCCCCCGCGGTGTGGATGACGCCGGTGAGGGGGTGGGCGGGGTCGATGGTGTCGATGATCTGCCGCAGGGCGGGGAGGTCCGAGGTGTCGGTGGCGGTGATGTGGACGGTGGCGCCGAGGGCGGTGAGTTTGTCGGTGAGTTGTTGGGCGCCGGGGGCATCAGGGCCGCGTCGGCTGAGGAGGTGGAGGTGGGTGATGTGGTGGGTGGTGACGAGGTGTTCGGCGATGAGGGCGCCGAGGGTGCCGGTGCCGCCGGTGATCAGGACGGTGCCGTTGGGGTCGAGGGTGCGGGGGATGTCCAGGACGAGTTTGCCGGTGTGTTTGGCCTGGCTCATATGGCGCAGCGCCTGGCGCGCCCTGGTCAGGGGCCATGCCTGCACCGGGGGCGGTTGGAGGACGCCCTGGGTGAAGAGGTCGGCCAGCGTGCCCAGCATCTCCGCGACCCGGTCCAGACCGGCGTCCGGAACCAGGTCGTAGAAGCGGTAGCGGACACCCGGGTACGTTGCGGCGATCTCCTCGGCGTCGCGCTTGTCGGTCTTGCCCATCTCGATGAACCGGCCGCCGTCGCCCAGCAGCCGCAGGGAGGCGTCGGTGAACTCGCCGGCCAGGCTGTTGAGGACCACGTCGACCTTCGGGAAGGTGTCCTCGAAGTCGAGGTCGCGGGAGGAGGCGCGGTGGGCGGCGTCGATGCCCATGGCCTCCAGGACGTGGTGTTTGGCGGGGCCGGCCGTGGCGTAGACCTCGGCGCCCAGGTGGCGGGCGATCTGCACGGCCGCCGTGCCCACGCCGCCGGTGGCGGCGTGGATGAGGACCGATTCGCCCGGTGTGAGTCCAGCGAGGTCCACCAGCCCGTACCAGGCGGTCAGGAACGTCGTCGGGACGGCCGCGGCCTGCTGGTCCGTCCAGCCGGGCGGGATCGGCACCACGGCGCGCGCGTCGGCGACGGCGACCGGTCCGAAGGCGCCCTCGAACAGCCCGAACACCTGGTCGCCGACGGCCACCGACGTGACGTCCGGCGCCACGTCGAGCACCACGCCCGCGCCCTCGCTGCCGCGGAACACCCCTTCGTCCGGGTACATCCCGAGCACGATCAGCACATCGCGGAAGTTGATGCCCGCGGCGCGCACGCCGATGCGCACCTGACCCGGCGCCAGCGGCTCCAAGACCTCGGGGCAGGCCACGACCGACACGCTGTCGACGGTGCCGGTGCCGTCGGACGCCAACCGCCAGGCCGGTTCGCCCGGCGGCGGCGCCAACTCCGCTGCCGCCGCCCGTTCCAGCCGTGGCGCCAGCACGCCTCCTCCGCGCACCGCCACCTGCGGTTCGTCGAGCGCCACAGCCCGCGTGACGGCGCCCCGTACCTGCTCGGGCCCCGCGTCGTCGTCGATGTCGCACAGCACGAAGCGGCCCGGGTTCTCCGCCTGGGCGCTGCGCACCAGGCCCCACACGGCGGCCGCCGCCGGGTCGGGCCGGGCGGCCCCGGGGCCGTCGTCGACATCCCCGGCCGCCACCGCGCCACGGGTGACGAGCACCAGCCGAGCCTCGGCGAGCTCCGGCCTCTCCAGCCACCGCCGCGCCGACGCCAGCACACCCGTCACCACGGCCGGCGCGGCGGCGGACGGCTCGTCGCCGCCCTCGGCCGCGGGCACGCCGACGAGCACCGTGTGGGGCGTGTCCCGGCCCGCGTCGTGGGCGGCGAACAGGGCGTCGAGGTCGGCGTAGCGGGGCAGCTGAGGATCTGCCGAGCCGAACGGGAGCGGGCCGCCTTCCAAGACCGCCCACCCCGGCGCCATCGTGTCGGACTCCGCTTCCAGGGGCGGCACAGGCGCCCACGCCACCTCGTACAGCCCGTCCAC CCGCCGCGCGCCCGTCTCGCGCAACTGCCGTGCGCTTGTCGGGCGTACGACCACGCAGTCCGCGCTGAGCACCGTATCGCCGGCCGCGTCGGCCACCATGAGCCGGAAGCCGTGCTCGAGCGGCGACATACGGACCCGCACCGCGCGGGCGCCGGTGGCCCACAGCGACACGCCGCCCCACGCGAACGGCAGCCGCAGTTGCCCGTCGTCGCCCTGGCCGTCCAGCGCCATGGGGTGCAGGACCGCGTCCAGCAGCGCGGGGTGGAGGGAGAACCCGTCGGCGCCCTCGTGGGCCGCCTCGGGCAGCACGACCTCCGCGAGCAGGTCGGCGCCCTGCCGCCAGGCGGCGGTCAGGCCCCGGAAGGCCGGGCCGTAGCCGTAGCCGATCGCCTCGGCGCGGCCGTAGAAGTCGGACACGTCCACCGGCTCGGCGTCGGGTGGCGGCCACGTCCCCGTAAACGCCGCGTCCGGCACGCCTGGCACACCCGGCGCCGTCGGAGTCGTCGGGGCGAGGTGTCCGGTGGCGTGGCAGGTCCAGGGCGACGCGCCGTCGTCCGCGTCCGGGCGGGAGTGCACCCGCACCTCGCGGCGGCCCTCCTCGCCGGGCGCGTCCACCACGACCTGGACCCGCAGCCCGCCGGACGCGGGCAGCGCCATCGGGATGTGGAGAGCGAGCTCCTCGATTCCGCCGCAGCCCGCCTCGTCGGCCGCCCGCAGCACCCACTCCACCAGGGCCGCGCCCGGCACCAGGGGAGTGCCGGCGACGACGTGGTCGGCGAGCCAGCCGCCGCCCCCGGCGGCGGGGAGCCGCCCGGTCATCACCAGCCCGCCGTCGGCCAGTTCGACCGCCGCGCCGAGCTGGGCGTGCGCCACCGAGGTGAGCCCGGCGCTCCGCAGATCGCCCGGGCCGCCGTCCATGCCCAGCCAGTAGCACCGCCGTTGGAAGGCGTAGGTGGGCAGGTCGGTGGTGCGCGGTGGCGGGTCGGCGGGGTACCAGACGTCCCAGGCCACGGCGAGCCCGGCGGTGTGCGCGGCGGCGACGGCCTGGGCGAGCCGGCGGGGCCCGCCCTGGTCGCGGCGGAGGGTGGGGATGGTGGCGGCGGAGATGTCCGCCGCCTCGAAGGCGTCCTGCATCCCGGGGGCGAGGACGGGGTGCGGGCTCACCTCGACGAAGTGGCGGTGTCCGCGGTCCAGGAGGGCGGCGAGGGTGTCGGTGAAGCGGACCGGCCTGCGGAGGTTGGTGAACCAGTAGTCGGTGTCGAGCCCGGCGGTGTCGAGACGCTCGGCGGTCACCGTGGAGTAGAAGGCGATGTCCGACGCGGCGGGCGTCAGCTCGCCCAGGGCGGAGACGATGTCCTCGCGGATGCGGTCGACATGGGGGCTGTGGGAGGCGTAGTCCACATCGATGGTGCGGGTCCGCGCGCCACGGGCCTCGGCGGCCGCCAGGACGGTGGCGATCTGCGCCACCGCGCCCGAGACGACCGTGGTGGCAGGGCCGTTGGCGGCGGCCAGGGTGACATCGTCGGCCTGGTCGCCCAGCTCCTCCAGGAGCCGGGTGGTGTCGTCGGCGCCGAGGGTGAGGGAGGCCATGGTGCCCTGGCCGGCCAGGGCGCGCAGGGCCCGGCTGCGCAGGGCCACGACCTTGGCGGCGTCGTCGAGGGTGAGGGCTCCGGCCACGCAGGCGGCGGCGATCTCGCCCTGGGAGTGGCCGATGACGGCGGCGGGGGTGACCCCGAGGTGTTGCCAGAGGGCGGCGAGGGAGACCATCACGGCCCAGAGGGTGGGCTGGACGACATCGACGCGCTGGGGGTCGGTGTCGGTGTCGGTGTCCGCGCCGCGCAGCACGGCGGTGAGTGACCAGTCCACGTACGGCGCCAGGGCTCGCTCGCACTCCGCGATCCGCGCCGCGAACACCGGTGAGCCGTCCAGCAGCTCCACGCCCATGCCCCGCCACTGCGACCCCTGGCCCGGGAAGACGAACACCGGGCCGGCGGGCGCCGCGATGGCCGTTCCCGCCACGACCGCCGGGTCGGGCTCGTCGGCGGCCAGCCTCTCCAACCCGGCCAGCAGCGAGTCCCGGTCGTCGCCCACGATCACGGCCCGGTGCTCGAACGACGACCGCGTCGTCGCCAACGACCAGCCCACCTCGGCCGGCTCGGTGTGGGGGAGCCGCGTGGCCAGGTTCGAGGCCTGGGCGCGCAGCGCTTCGGCGGTGCGCGCGGAGAGCACGAGGGGCAGGACGCCGGGCCGGGAGGTGCGCTCCGGCTCGGGCTGTTCGGGGGCTTGTTCGAGGATGAGGTGGGCGTTGGTGCCGGAGATGCCGAAGGAGGAGACGGCGGCGCGGCGCGGTCGGTCGGTGTCGGGCCACTGGGCGGCCTCGGTGAGGAGGCGGACGTTTCCGGTGGCCCAGTCGACGTGCGGGCTGGGCTCGTCCACGTGCAGGGTGGCCGGGAGGTGGCCGTGGCGCAGGGCCATCACCATCTTGATGACAGCGGCGGCACCGGCGGCGGCCTGGGTGTGGCCGATGTTGGACTTGATCGAGCCCAGCCACAGGGGCCGGTCCTCGGGGCGGTTCTGTCCGTAGGTGGCGAGGAGGGCCTGGGCCTCGATCGGGTCGCCCAGGGTCGTGCCCGTGCCGTGGGCCTCGACGGCGTCCACGTCCGTCGGATCCAATCCGGCCGTGGCCAGGGCCTGGCGGATGACGCGCTGCTGCGAGGGGCCGTTGGGCGCGGTCAGGCCGTTGCTCGCGCCGTCCTGGTTGATCGCCGAGCCCCGCAGGATCGCGAGCACCCGGTGCCCGTTGCGCCGGGCGTCCGACAGCCGCTCCAGCACCAGCAGCCCGGCGCCCTCGCTCCAGCCGGTGCCGTCGGCGGCTGCGGCGAACGGCTTGCACCGCCCGTCCGGGGCCAGGCCGCGCTGCCGGGAGAACTCGACGAACGTGTTCGGCGTGGCCATCACCGTCACCCCGCCCGCGAGCGCCAGATCGCACTCACCCTGCCGCAGCGCCTGCGCGGCGAGGTGCATTGCCACCAGCGACGACGAACACGCCGTGTCCACCGTGAGCGTCGGCCCCTCCAGACCGAAGGTGTAGGCCACCCGGCCGGACGCCACGCTCGGCGTGGAGCCCGTCATGATCTGGCCCTCGTACCCCTCCGGGATCCGGCCGAGGAACCGCGCGCCGTAGTCGTCGTACATGATGCCGGTGACCACCGCCGTCCGGCTGCCGCGCAGCGCCACCGGATCGATGCGGGCGTGCTCGAACGCCTGCCACGCCGTCTCCAGCAGCAGCCGCTGCTGCGGGTCGGTGGCCAGCGCCTCCCGGGGGCTGATCCCGAAGAACTCCGCGTCGAAAAGGGCGGCTTGGTGCAGAAAGCCGCCCTCCCTGGCGTAGCTCTTGCCCGTCCGCTCGGGGTCGGGGTCGTACAGCCCGGCCACGTCCCAGCCGCGGTCCTCGGGGAACGGCCCGATCGCGTCCACCCCGTCGGCGACCAGCCGCCACAGCCCGTCCGGGGA GTGCACATCGCCCGGGTAGTGGCAGGCCATGCCGACGATCACGACGGGGTCGTCGGCGTTTCCGGCCCGGGTCGGCGCGCCCGCTCCCGGCGGCAGCGGCGCGGCGGCCGCGGTGGCGCCGAGCAGTTCGGTGCGCAGATGGCTCACCAGGGTCTCGGGCGTCGGGTAGTCGAAGACCGCGGTGGCCGGCAGGCGGACGCCCGTCGCGTCGTGGATCGCGTTGCGCAGCTCCAGCGCGGTCAGCGAGTCGAGGCCGAGCTGCTGGAAGGTCCGGCCCAGTTCGAGGGTTTCCGGCTCGGGGAGGGCCAGGACGGCGGCGATCCGGTCGCGTATCAGCTCGCCGATCACCTGGTCCTGCTCGGCCTCCGGCAGCCCGGCCAGCCGCCCGGCCCAGGAGGGGGACACGGCGTCCGGCGCGGCCGTACGCGCCGTACGCACCGGGACGCGCACGAGGCGGCGCAGCAGCGGGGGCAGCGTTCCGGCGGCGGCCTGCTCGCGCAGCACGGCGAGTTCGAACCTGGCCGGGACCAGCACGGGCGGCGCGGTGGCGAGCGCCGCGTCGAACCGGGCCAGGCCCTGCTCCCCGGACATCGTGGCGGTGCCCGAACGCGCCATCCGGGCCAGATCCGCGGTGGCGAGGTGTTCGGTCATCCCCTCGTCCACCCCCCACAGGCCCCAGGCCAGCGAGGTCGCCCGGCCGCCCGCCGCGCGCACGTGTTCGGCGAGGGCGTTGAGGAACGCGTTCGCCGCGCCGTAGTTGCCCTGCCCGCCGCTGCCGAGCACCGAGGCGGCGGAGGAGAACAGCACGAACGAGGCGAGGCCCGCGCCCTCGGTCAGCCGGTGCAGATGCCAGGCCGCGTCCACCTTCGGCGCCAGGACGGCGTCGAGTTGGCCGGGCGTCAGCGAGGTGACCACGCCGTCGTCGAGCACGCCCGCGGTGTGCACGACCGACGTCAGCGGGTGCTCGGCGGGGACGGTGGCGAGCAGCGCGGCCAGCGCCTCGGGGTCGGAGGTGTCACAGCTCTCGACGCGTACGTCGGCCCCGAGCGCGGCCGCGAACGCGTCGATGCCCTCGGCGGCCGGACCGCTCCGGCTGACCAGCAGCAGATGCCGGACACCGTGGGTTTCGACGAGGTGCCGGGCGAGCAGCCGGCCGAGCGAGCCGGTGCCGCCGGTCACCAGTACGGTGCCCTCGGGGTCGAACGCCGTCGGCGCCGTGTCCTCGTCGGGGAGCGCCGCGACCAGCCGGGGCACCAGCACCTGCCCGTCGCGGAGCGCCAGTTGCGACTCGCCCGCGGCCGTGGCGGCCGCGACCGCCGCCGCCAGCGCGTCCCGGGACGCCGGGCTGTCATCCGTGTCCACCAGCACGATCCGCCCCGGGTGTTCGGCCTGCGCGGCGCGCAGCAGGCCCAGCATCGCGGCGCCCGCCAGGTCGGCCGGAGTGTCGGCCGGGTCGGCGGCGATGGCTCCCGTAGTGAGCACCACCAGACGGCCGGTGGCGAGGGCGTCGTCCGCCAGCCACTCCCGCAGCCACGCGAGTACGTGGTGCGTGGCTGCGCGCACCCGGGCGGGCGGCGCGTCGCCATCGGGGGCGCAGAGGCAGCCCATGGCGATCAGGTCGGGGGCCGGGGCCCCGGCGGCGACCGAGGCGCTCAGCGCGGCGAGGTCCGGATGGCGCGCCACGTGCTCATCGGGCAGTGCGGCGGTGGCGCCGTCGGTCCGGTCCGTGCCGTTCGCGTCGACCACGCCCCAGCGGTCCGCCGTCGCCGTCGGCACGGCGGCGGGCAGCGCGAAGGGCTCCCAGTCCAGCCGGTACAGCGAACGGTCGGCCTGCGCCGCCCCGGACCGCCACCGCTCGGCGGAGACGGTGCGCAGCGTGAGGGCGTCGACCGTGGCGACCTCTGCGCCGGCGTCGTCGGACAGCCGCATGGCCACGCTGTCCGGCCCGGTCGGCGTGATGCGGACCCGCAGCCGGGACGCGCCGCGCGCCGACAGCCGCACACCGCCGAAGGAGAACGGCAGCCGGACCGTGCCCGGCGCGCCGCCCGCCTGGTCCTCGCCGGTGAACAGCGCGCCGATCGGGTGCAGGGCGGCGTCGAGGAGGGCGGGGTGGACGGCGTACGCCCGGCCGTCGTCCCGCAGGCCGGGGTCCAGGCTCACCTCGGCGAACATCTCCTCGCCGACTCGCCACGCCGCGCGCAGCCCCGTGAACACAGGTCCGTAGCGGTACCCCTCGTCGGCCAGCCGCTCGTACAACCCGGCCACGTCGACCTCTTCCGCCCCGGCCGGTGGCCACGGGGCAGGCGGCGCCGCGGGCTCGGGAGTGGCACTGGTCCCCGCGGCGGTCAGCGTGGCGGTGGCGTGCCGGTGCCAGGGTCCGTACGGCTCATCGGCACCGTCCGACGCCTCGGCGCCGCCGCCGGACGGGCGCGAGTAGACGGTGACGGCCCGTCGGCCGGCCTGGTCGGCTGCCCCGACGGCGACCTGGAGATCCACCGCCCCCACCGGGGGCAGCAGCAGTGGCGCCTCCAGGGTGAGGTCCGCGATCCGGTCGCAGCCGGTCCGTTCGCCCGCCTGGAGCGCGAGTTCCACGAAGAGCGTGCCGGGCAGCGGCACCGCGTCCACGATGGCGTGGTCGGCCAGCCAGGGCTGACCGCGCAGCGACAGCCGGCCGGTCAGCAGCAACCCGCCGTCCGCCGGTTCGAGCGCGGCGCGCAGCAGAGGGTGGTCGCTCGCCTCGAGCCCGGCGGCCCGTACGTCCGCCTCCGCTTGCGGGGCGTCCCGCCAGTAGCGGCGGCGGTCGAAGCGGTAGCCGGGCAGGTCGGTGGCCGGGGTCCCCGGCGGGAAGGCGGCGGCGAAGTCGACGGTCTCGCCGTCCACATGGAGCCGCGCGAGCGCGGTCAGCAGCGTACGGGACTCCTCGCGGCCGCCGCGCAGCGCCGCGGCGAGCACCGGCGCGCCCGCGCCCCCCGATCCTCCGCCGGCACCTTCCGCTGCCAGGCAGTCGCGGGCCATGGTGGTGAGCACGGCGTCCGGGCCGAGTTCCAGGAAGCGGGTGACGCCCTCCGCGCGCAGGGCGGCGATGCCGTCGCGGAACCGCACCGGCTGCCGCGCGTGCCGCACCCAGTAGTCGGGGTCGCACAGCTGCTCGGCGCCGGCCAGCGCGCCGGTCACGTTCGAGACGACCGCGATCCGCGGCGGGGCGTACGTCAGCGAGGCGGCGACCCGCCGGAACTCCTCCAGCATCGGATCGATCCGCGGCGAGTGGAAGGCGTGGCCGACCCGCAGCCGCCGGGTCCGGTGTCCGCGCTCCCGTATCGCGCGGGCGACCTCCTCGACCGCCTCCTCGTCGCCCGACACCACCACCGACGTCGGCGCGTTGACGGCCGCCAGCGCCACCCGGTCGGCCCGTCCGGCGAGCAGCGGCGCCACCTCTTCCTCGGCGGCCTCCAGCGCGATCATCGCGCCACCTTCCGGCAGCGCCTGCATCAGCCGCCCGCGGGCGGCC ACCAGCCGCGCCGCGTCCGGCAGCGACAGCACCCCGGCGATGTGCGCGGCGACCAGCTCGCCGACCGAGTGCCCCGCCAGATAGCCGGGCACCACGCCGAACGACTCCACCAGCCGGTACAGGGCGACCTCGACCGCGAACAGCGCGGCCTGCGTCACCCCCGTCGCGTCCAGCGGACCGGCCTCGTCCCCGTCCCCGTCGGCGGGCGCGAACACCAACTCCCGCAGCGAACGCCCCAGGTGGGGGTCCAGCGCCGCACACGCCTCGTCGAACGCGGCGGCGAACACCGGATAGGTCCGGTACAGCCGGGCGCCCATGCCGGGGCGCTGGCTGCCCTGCCCGGTGAAGAGGAACGCGCTGCCACCGAGCCGCGCCGGGGCGGACGCCCGCGTCACATCGGGATGTGTACGCCCCTCGGCGAGCTCGGCGAGCCGGGCACACAGCTCCTCGCGGTCCGTGCCCAGGACGACGGCGCGGTGGTCGAGCGCGGACCGGGTGGCGGCCAGCGACCAGCCCACCGCGTCGACCGGGAGGTCCGGGCGCGCGGCGAGGTGTTCGGCGAGCGCTGCGGCCTGCGTGCCCAGCGGCGCCGCGCCGCGCGCGGAGAGCACCCACGGCACGGCGGCGCCCTCCGGGCGTGGCGGCGTACCCGCGGGCAGCTCCGTGTCCGTGTCCGGCAGGGGCGGGGCCTCTTCGAGAATCAGATGCGCGTTGGTGCCGCTGATGCCGAAGGACGACACACCGGCCCGGCGCCGCCGCTCCCCGGTCGGCCACGCGACCGGCTCGGCCAGCAGCCGCACCGCTCCGCCGTCCCAGTCGACCACGGGCGTGGGCCGGTCGGCGTTCAGGAGGGCGGGCAGCGAGGCGTTCCGCAGCGCCATGACCATCTTGATCACGCCCGCGACACCCGCCGCGCCCTGGGTGTGTCCGATGTTCGGCTTGACCGCGCCCAGCCACAGCGGCCGGTCCGCGGGCCGCTCCTGACCGTACGTGGCCAGCAGCGCCTGGGCCTCGATGGGATCGCCCAGTGTCGTGCCGGTGCCGTGTGCCTCGACCGCGTCCACGTCCGCCGGAGCGAGCCGGGCGCCGGCCAGCGCCTGCCGGATGACGCGCTGCTGCGACAGTCCGTTGGGCGCGGTCAGCCCGTTGCTCGCGCCGTCCTGGTTGATCGCCGAGCCGCGCACCACGGCCAGGACGCGGTGACCGTTGCGCCGGGCGTCCGACAGCCGCTCCAGCACCAGCAGCCCCACGCCCTCGGCGAACGCGGTGCCGTCGGCCCCCTCGGAGAAGGCCTTGCACCGGCCGTCCGCCGCCAGCCCCCGCTGCCGCGCGAACTCCGTGAACACGTAAGGAGTGGTCATGACCGTCACCCCGCCCGCCAGCGCCAGTTCGCACTCGCCCTGACGCAGCGCCTGCGCGGCGAGGTGCATCGCCACCAGCGACGAGGAACACGCCGTGTCCACCGTGACCGCGGGCCCCTCCAGACCCAGGGTGTACGCCACCCGGCCGGACAGCACACTGGGCGCGTTGCCGGTGACCAGATAGCCCTCCGCGGCCCGGTCGATGTGCGGGGTACCGAAGCCGGGCAGGGCGGTGCCCGCGTACACGCCGACGGGCGCGCCCTTCAGCGACCCGGGCGCGATGCCCGCCCGCTCCAGCGCCTCCCAGGCCACCTCCAGCAGCAGCCGCTGCTGCGGGTCGATCGCGGCGGCCTCCCGGGGGCTGATGCCGAAGAACTCCGGGTCGAACAGGTCGGCGTCGTGCAGGAAGCCGCCCTCGCGGGCGTAGCTGGTGCCCGGGTGGTCGGGGTCAGGGTGGAACAGCCGCTCCAGGTCCCAGCCGCGGTTGTCCGGAAACGCGGTCATCGCGTGCCGGCGGGCGGCGACCAGGTCCCACAGGTCCTCGGGCGAGGCCACCCCGCCGGGGTAGCGGCAGGCCATCGCCACGATGGCCACCGGCTCCCGCTGCCCGTCCTCGACCTCGCGCAGCCGCGCCCGGGTGTCGTGCAGATCGGCGGCGACCCGCTTGAGGTAGTCGACCAGCCGCTCTTCGTTGGTCTCTGCGGTCACGGCATGTCCCTCCCCACCCGCTCAGTCTTGGCGCGCTTCAACGCGCGGGCTTCCTTCGTCACTGCTCGCTCCTTCGTCGCTGCGCGGCTCCTCAGTCCAGCCCGCACAGCGCGCCAAGACACGCTCACTTCGCCTGCCGCGCTCGTCGAGGCTTCTCGGGCTCTCTCGGCGTTCGTGCCAGACCGGGCTCATTGCACTCCGAGTTCCTTGTCGATGAAGTCGAGGACCTGGTCGGTCGTCGCGGCTTCGAGCCGCTCGGCCGCGCTGCCGCCGTTCCGGCCCGGGGCGTGGGCCGCCTTCCAGGCGGCCAGCAGCGACTCCAGACGCGCCGTCACCGCGCCGGGGTCGGCCCCTCCGGCGCCGTTCACACCGCCGTTCGCGCCGCCGTTCGCACCGGACGCGGTGAGCGCCGTCAGCGTCTCCTCCAGACCCGTCAGCCGGCTGAGCACGGGCGCGTAGAGGTCGGGCGCGGTCGCCGCCGCCTCCTCGGGGGCCAGTCGGCCGCGCAGATGCTCGGCCAGCGCCACCGGCTCCGGGTAGTCGAAGACGACGGTGGCGGGCAGCCGTACCCCGGTGGCGGCGGTGACGCGGTTGCGCAGCTCGACGGCGGTCAGCGAGTCGAACCCGAGCTCCTTGAACGCCGCGTCCGCCGGTACGGCGTCGGGGTCGGCGTGTCCGAGCACGGTGGCGGCGTGTTTGCGGATCAGATCGGTGAGCAGGCGGCGCTGTTCGGCCGGGGCGAGACCGCCCAGCCGGGCCGCCCAGCCGCCGGGCTCCTCGCCCGCCGCCGCGGTCGGCCGCGCCGCGCCCCCCGGCCCGCCGGAGCGGGCCCCCGCCGCCGACGCCTCCGCTGCCAGGGCGCGCAGCGGCACGGGCAGGGCCGCGAGGGGCTGCGCGGCCAGCGCCCGGGTGTCCAGGTTCAGCGCGAGCAGCGCCGGCCGGCCGTGCTCCACCGCCGCGTCGAACAGCTCCTGCCCCTGCTCGGCGCTGTTCGGAACGATGCCCTGCCGGGCGATCCGCGCCACGTCCGACTCGGTCAGCCCTCCGGTGAGCCCGCTCGCGGTGGACCACAGGCCCCAGCTCACCGACACGCCCGGCAGACCCTCGGCGTGGCGCCGCTCGACCAGGGCGTCGCAGAAGGCGTTGGCGGCCGCGTAGTTGGCCTGCCCAGGGGTGCCGAGGGTGGACGCGAACGAGGAGAACAACACGAACATGCCCAGCGGCAGCCCGGCCGTCGCGCGGTGCAGATGGGCCGCCGCCGCGGCCTTGGCCGCCCAGGCCTCGGCCATGCGCTGCTCGTCCTGCGCGGGCAGCATCGCGTCCGCCACCACACCCGCCGCGTGGACGACCCCGGTGAGCGGATGCGCCGGGTCGATCCCCGCGATTGCTTCACCCAGCGCGTCGGCGTCCCCGACGTCCACCGCGGCCACCCGCACTCGTGCGCCGAGTTCGG TGAGCCGGGCCGTCAGCTCCGCCGCGCCCGGCGCGTCGGGGCCGCGCCGGCCGAGCAGCAGCAGATGCCGAACCCCGCCCGAGCGCACCAGGTGTTCGGCGATCAGGCCGCCGAGGGTGCCGGTGCCGCCCGTGATCAGCACCGTGCCGTCCGGGTCCGGCGCGGGCGCCACGTCCAGCACCAGCTTGCCGGTGTGCCGGGCCTGGCTGAGCTGCCGAAGCGCCTGCCTGACCTGGCCGAGCCGCCACGCGGTGACGGGCAGGGGCGTGAACGCGCCGGTGGCGAACCGCTCGCCCACGGCGGCCAGCAGCCGCCCGATGTGCTCCGGACCGGCGTCCGCGATCAGGTCGAACGCCCGGTACGCCACGGAGTGGTCCCGCGCGACCCGCTCCGGGTCGCGTACGTCCGTCTTGCCCATCTCGATCAGCCGGCCGCCGGGCGCGAGCAGCCGCAGCGAGGCGTCGGTGAACTCCCCGGCCAGGCTGTTGAGGACGAGGTCGACGCCCCGGCCGCCGGTCGCCGCCGAGAACACCGCCTCGAACTCCAGGTCCCGAGAGGACGCCCGGTGTGCGGCGTCCACGCCCATGCCCTCCAGCACCGGGTGCTTCGCGGGGCTCGCCGTGGCGAACACCTCCGCGCCCAGGTGCCGGGCGACCTGGACCGCGGCCATGCCCACTCCGCCGGTCGCGCCGTGGATCAGCACGGTCTCGCCCGGGCGCAGCCCGCCCAGCTCCACCAGGCCGTACCAGGCGGTGAGGAAGGCCACCGGGGCGGCCGCGGCCTCCCGCAGGCTCCAGCCGTGCGGCACGCGGGCGACCATCCGGGCATCGGCCACCGTCACAGGGCCGAACGCACCGTCGAACAGGCCCATCACACGGTCGCCCACGGCCACGCCGGTCACCCCGTCGGACACCTCCACGACGACCCCGGCGCCCTCGTTGCCGATCGACGCCTCGGCGTCCGGGTACATGTCCAGGGCGATCAGCGCGTCGCGGAAGTTGAGACCGGCGGCGCGCACGGCGATCCGCACCTCGCCGGGCGCGAGCAGGGCCGAGGCCTGAGGGCTTGAGGCCGTGGTGAGGTCGTCCGGGCCGGTCTTTCCGGCCACCACACGCCAGGCCCGCTCGCCCGGAGGGGCCGCCAGCTCCGGCTCGGTGTCCGCCCGGGCCAGCCGGGGCACCAGCGCCCGCCCGCCCCGCAGCGCGAGCTGCGGCTCGTCGGCGCGTACTGCCTCCCGTACCGCTTCGGCCAGTCCGGTGTCGGCGTCGCAGCCGGTGTCGCAGCCGGTGTCGATGAGGAGGAAACGCCCCGGGTGTTCCGTCTGGGCGCTGCGTACGAGCCCCCACACCGCTGCCGCGGCGGGGTCCACCGGCTCCTCGCCGGCGCCACCGGCCCGCACCCCAGAACGCGTGACCACGGCCAGCCGTGCGCCCTCGGTCACGCTCCCGGCGGCCAGCCACCGCTGAGCGAGCGCCAGTGTCCGGCGCGCGGTGGCCAGACCCGTGTCCGCCCCCGCCGGGTCCGGGCCGGTCCGCACCGGGGCGACGACGACATCCGGGACGGGGGCGCCGCCGTCCACGGCGTGGCACAGCGCGTCGAGATCGGCGTAACGGGCGGCATCGGCCAGGCCCGGGGGGCCCTGGGCGTCATTGCCCACCACCGCAAGTGTCACCGGAACAGCCCCGGCCTCTTCAACCGCCGCAGCGGGAAGGGGCGTCCACTCCACCGCGAACAGCCCGTGCGGCCCCCGCGCACGGCTCGCCGCGCGCAGCTGACGGCCGTCGGTCTCGCGGAGCGCGACCGCGTCCGCGGTCAGCACCGGGGAGCCCGTGGCGTCGGCCAGCAGCACCCGCAGCCCGCCCGGCCGCGCCGACAGCCGGACCCTGAGCCGCGTGGCGCCACCCGCCCACAGCCGCACCCCGCTCCAGGCGAACGGGAGCAGCACCGCCGCGCGCCCCGCGTCGGCGCCCGGCGCGGCGGTGTCCAGGTGACCGGTCAGCAGCACCGGCTGCATCGCCGCGTCGAGCAGGGCGGGGTGGATACCGAAGCTGCCCGCCTGGTCCGCCGCCTCCTCGGGCAGCACCACCTCCGCCAGCGTGTCCTCGCCGTCGCGCCATACGCTCCGCAGCCCCCGGAACACCGTCCCGTAGCCGTAACCCGCCGCCGCGGCCCGCTCGTAGAAACCCTCGATCGCCACCGGGCGCGCCCCCGGCGGCGGCCACGCCCCGCCCGGCTCCGGCGCCGGGAGCGGCCCGTCCGCGCCGGGGAGCAGCGTGCCCACGGCATGGCAGATCCAGGTGTCCGGCCCGCCGGAGTCCTCCTCGGGCCGGGAGAAGACCCGCACCGGACGGCGCCCGTCGACCTCCGCGGCGTCCACGACCACCTGGACCACCAGCCCGCCCGAGGCGGGAAGCGCCACCGGGACCTGGAGCGCCAGCTCCTCGACGCCGCCGCAGCCCGCCTCGTCGGCCGCCCGCAGCACCCACTCGACCAGCACCGCGCCGGGCAGCAACGGCAGGCCCGCGACAATGTGTTCGGCCGGCCAGCCGCCGCGCGCCGCCTCCGGCAGCCGCCCGCTGAGCAGCAGGCCGCCGTCGGCCAGACCCACGGCCACCGGGAGCAGCGCGTGTCCGATCGCGGTGAGCCCGGCCCCCGCCACATCGCCGGGACCGGTGCCGCCGCCGGTCGGCAGCCAGTACCGCTCCCGCTGGAAGGCGTACGACGGCAGCGGCACCGCCGTGGGCGCGGGCGCCCCGGCGTACCAGCTCGCCCAGTCGACCGCCACGCCCGCGACATGCGCCTCGGCCAGCGCCCGGCACAGCCGCTCCGGGCCGCCCTCGTCGCGGCGGAGCGTGCCGATGGCCGCGGCCGGGGAGCCCGCCGCCTCGATGCACTCCTGAAGGCCGAGCAGCAGCACGGGATGCGCGCTGACCTCGATGAACACCCGGTAGTCGTCCGCCAGCAGCGCCTGGACCGCCTCGGCGAAACGCACCGGCTGCCGCAGGTTGGTGAACCAGTAGCCGGCGTCGAGGCCGGAGGCGTCCATACGGGCACCGGTCACCGTCGAGTAGAAGGCGACATCGGACGCGGTGTCGGACGCGGCGCCGGACACCCCGGCGAGCACCTCGGCCAACACGCCGGCGATGCCGTCCACCTGAGGGCCGTGCGAGGCGTAGTCCACGTCGATCGCGCGGGCCCGCAGCCCGTCGGCCCGGGCGGCGGCCACCAGCTTCGCGATCTGCTCCGGCGGCCCGGACACCACCGTGGACGACGGGCCGTTGACCGCCGCGACCGCCACGTCCGGCGCGTGCAGGGCGGTCAGCTCCTCGGTGCGCTCGACGCCACAGCCCATCGAGGCCATGGCGCCGTGCCCGGCCAGCGCCCGCAGCGCCCGCGCCCGCAGCGCCACCACCCGGGCGCCGTCCTCCAGCGTCATCGCGCCCGCGACACACGCCGCCGCGATCTCCCCCTGCGAGTGGCCCACCACGGCGGCCGGTACCACGCCGTGCGCCTC CCACACCGCGGCCAGCGACACCATGACCGCCCAGAGCACCGGCTGCACCACATCGACCCGGCCGATGTCGGCCGCGCCCTCCGCGCCGCGCAGCACATCGGTCAGCGACCAGTCGACATGCGCGGCCAGGGCCCGTTCGCACGCGGCGACGCGCGCCGCGAAGACGGGGGAGGAGTCCAGCAGCGCCACGCCCATACCGAGCCACTGCGACCCCTGCCCGGGGAACACCATGACCGGGCCCGCGCCCACGTCCGCCGCCACGCCCCGCACCACCGCGGGGCCCGGCTCGCCCGCCGCCAGCCCTTCGAGCGCGGCCAGGAGGTCCGGCCGGCCACGGCCCACCACGACGGCCCGGTGCTCGAACGCCGTGCGGCGCGTCGCCAGCGACCACCCCACGTCGACGGCGCCACTCGCCACCCCGTCCGCCGTCAGGAAGGCGCCCAGACGCGCGGCCTGGTCCCTCAGCGCCGCCTCGGTGCGCCCCGAGACGAGCCAGGGCACCACGCCGCTCTCGGCGTTGACCCGCTGCTCATCTGTCTGACCGTCGGCGTCCGGGGCCTGTTCGAGGATCACATGCGCGTTGGTGCCGGAGACGCCGAACGACGACACCGCCGCCCGGCGCGGCCGCTCGGCCACCGGCCAGTCCCGGGGCTCCGTCAGCAGCCGCACCGTGCCACCGCTCCAGTCCACGTGCCGGGTCGGCTCGTCGATGTGCAGGGTCCGGGCCAGCTTGCCGTCCCGCAGCGCCATCACGGTGTTGATCGCGCCCGCGACGCCGGCCGCCGCGTGCGTATGGCCGATGTTCGACTTGATCGAGCCGAGGTGGAGCGGCCGGTCCGCCGGGCGGTCCGCCCCGTACGTGGCGATCAGCGCCTCCGCCTCGATGGGGTCGCCCAGCCGGGTGCCGGTCCCGTGCGCCTCGACCATGTCCACGTCCGCGCCGGACAGGCCGGCTCGCGCCAACGCGTCCCGGATCACGTTCTCCTGGGCGACGTCGTTGGGCGCGGTGAGCCCGTTGCTCGCGCCGTCCTGGTTGATGGCCGAGCCGCGCACCACGGCCAGGACGCGGTGTCCGTTGCGCCGCGCGTCGGACAGCCGCTCCAGCAGCAGGACGCCGGCGCCCTCGGAGAAGCCGGTGCCGTCGGCGGCGGCCGCGAACGGCTTGCACCGGGCGTCGGGGGTCAGCGCACGCTGCCGGCAGAACTCGGTGAACATCAGCGGCGTGGACAGCACGGTGACCCCGCCCGCGAGCGCCAGGGAGCACTCGCCCTGCCGCAGGGCCTGCCCCGCCAGATGGATGGCCACCATCGACGACGAGCACGCGGTGTCCACGGTGACCGCGGGTCCCTCGAAACCGAAGGTGTACGCCACGCGGCCGGAGATGACGCTGGTGAGGCTGCCCGTGGTGGCGTAGCCCTCGAAGCCCTCCGGGATCCGCGGCAGGCCGGAGATGTAGTCCTCGCTCGACACCCCCACGTAGACGCCGGTCCGGCTGGCGCGCAGGGCGCCGGGCACCACCCCGGCGGACTCCAGCGCCTCCCACGACAGTTCGAGGAGCAGCCGCTGCTGCGGGTCCATCGCCAGCGCCTCGCGCGGGCTGATCCCGAAGAACCCCGCGTCGAAACGGTCGACGTCGTGCAGGAAGCCGCCCTGGCTCACATAGCTGGTGCCGAAGTTGGCGGGATCCGGGTGGAAGAGGCCGTCCAGGTCCCAGCCGCGGTCGGTGGGGAAGTCGCCGATCGCGTCGCCGCCCGACAGCACGAGGCGCCGCAGGTCCTCGGGGGACCGCACCCCGCCGGGGAAGCGGCAGGCCATGCCGACCACCGCGACCGGCTCCTGCTCCCCCTCCTCCAGCTCCCGCAGACGGGTCCTGGCGTCGTGCAACTCGGTGGTGACCCGGCGCAGGTACTCGACCAGTTTCTCTTCGCTGACCATGTCGTCACCTGCCTTCAGTGGGAGGGGCGCGGACGTGCGTCGGAGAGCACGCCGAGCTCGTTGTCGATGAAGTCGAGGATTTCGGCGGCCGTGGCGGACGCCAGCTTGTCCGCGGCGTCGAGCGTGGAGCCGTTGCCGTTGCCGTTGTGCGGAGTGTCCTGTGCGCTGTTCGGGGTGTCGGGAGCGAGCTGCTGCTCGATACGCCGGGCCAGCGCCTCCGGCGTGGGGTGCCGGAAGATGACGGCGGCGGGCAGCCGCAGCCCGCTGGCCGCCACCAGCCGGTCGCGCAGCTCGACGGCGGTCATCGAGTCGAAGCCCAGCTCCTTGAACGACGAGTCGCCGCGCACGGCCTTGGGGTCGCTCTGCCCGAGCACCGCGGCGGCGTGGTCGCGCACCAGCTCCAGGACGGCGCGGCGCCGTTCGGCGGGCCCGAGGCCGGCCAGCCGGCTCGCCAAGTCGGCGGTGCGGCCCGGGGCCGCGGCCACCGGCCGGACCGGTGCCCGGCCACCCGCGCTCGCGGCGAGTGCGCGCAGCGCGGCCGGCAGCTCGTGGCCCGGACGGGCGGCCAGGGCCCGGACCCGGATGTCGGCCACCACCAGCCCGGGGCGGTCGGCGTGGCAGGCGGCCCGGAACAGCGCCGCCCCGCGGTCCGCGCCGAGCACCCCCGCCCAGCGCCCGCCGGAGCCAGCCCCGGAACCCGCCCCGGAGCCGGCATCGGCGCCTATGGCAGCGGCCCACGGCCCGAACGCCACCGCGAGGCCGGGCAGCCCGGCCCGGCGCCGCGCCGCCATCACGGCGTCGCAGAACGCACCGGCCGCGGCCCGTTCGGGCGACACCGGACCGCCCCAGGTCGCGGCGGCGTCCGACAGGGTGACAAACCGGACCGACGGCAGTCCGGCGGTGGCCCGTTGCAACGCCGTCACCGCCCCGGCCACCGCGGCCCACCCTCGTACCGTGTCACCCGCCGACTCGACGGCGACCGGCTCGATGGCGGACGACAGCCCCGCGACGTGGACGACGCCGGTCAGTGGATGGGCCGGCTCGACCCGCGCCACCACCCGTTCCAGCGCGGCGGCGTTGAGCGGGTCGACCGTGACCACGCGCACCCGCGCGCCCCACCGCTCCTCACCGCTCCCGCCGTCGTCCGTACTCTCCGCGCCGCCGAAGCGGACCAGTAGCAGCCGCCGGACGCCCTCGGCGCGGGCCAGATGCTCGGCGACAGCCTCGGCGAGCGGGCTTCCGGCGTCCGTGACGAGGACCGTGCCCTCCGCGTCGAACGCCCCGTCGCCCGGGGCCGCGGCGGAGCGCGCCAGGCGTGGCGCGAGCAGTGTGTCCCCGCGCAGCGCGACCCTCGGCTCGTCGAGCGCGGCCACCCGCGCCACGGCCGCCGGACCCGCCTTGGCGCCGATGCCGGTGTCGGTGTCGGTGTCGATGAGCAGGAAGCGGCCGGGATGTTCGGCCTGGACACCGCTCGCGTACCCCCAGGCCGCCGCCGCGGCCGGGTCCACCGGATCGCCCTCGGAGCCGCCGGGCGCCACCGCGCCCCGGGTGAGCACCGCGAGCCGGCTGACCGCCAGCCGC GGCTCGGCACACCAGCCGCGCAGCAGCGCCGCGACGCCCCGTACGGCGGACAGCGCCGCCTCGTCCGGCGCGGCCCCGGGGTGAGCGGCCGGTGTGCGCCACAGCACCAAGGACGGCGCGGGCGCTCCGGCGTCCAGCGCGGCCAGCAGCGCCGGAAGGTCCGCGTACCGATCGGCGTTCGCGTCGGCCGACTCCGTCTCCGACTCCGACTCCGTCAGCGCCTCGCCCAACACGGCCACCCGCCCCGTGTCGTCCTCGGCGCCGGAGGGCTCCGCGGGGGCCACCGCGGTCCAGTCGACGGCGAAGAGACCACCCGCGGCGGCCCCGCTCGCCTCGCGTATCTGCCGTACGCCACGCGGGCGCAGCACCACCGACTCGATGCTCAGCACGGGCGCCCCGGCCGGGTCGGACACCTCAATCCGCAGCTCGCGTTCTTCGTCTGCTTCGTCCCACGGCGACAGCCGGACCCGCGCGGCGCGCGCCCCGGTCGCCCACAGCCGGACCCCGCTCCACGCGAACGGCAGCCATACCCGGGCCTCAGCGTCGTCGGGGGCGGTCAGCAGCGCGGGCTGCAACGCGGCGTCCAGCAGCACCGGGTGGATGCCGAACCCGGCCGTGTCCTCACCACCTTCACCGTTGTCACCGCTCTCACCGGCATCGTCGGGCAGCGCCACGTCGGCGACCAGGTCGGCGCCGTCCCGCCACAGCGCCCGTACGCCGCGCAGCGCGGGCCCGTAGCCGTAGCCGCGCCGCTCGGCCCGCTCGTACAGCTCCGCCGGATCCAGGGCCTCGGCGCCCGCGGGCGGCCACGGGACGACCGGCGTTCCGGCCGCCGCCGGGCCGGACCGGCCCCCGGGGACCAGCGACCCCACCGCGTGACACGTCCATTCCCCACCGGTGCCGACACCGCCACCGGCGTCGGCGGGCCGGGACGAGATCCGTATCCCACGGCGGCCGGACCCGTCCGGCGCCGCGATCGCGACCTGCGCCAACACGCCCCCGGGCGCACCCGACAGCTCCAGCGGCGCACGCAGCAGCAGCTCCTCCAGCGACGCCCCGCCCGCCTCGTCGGCGGCCAGCAGCGCCCAGTCGACCAGGACCGCGCCGGGCACCAGCCCGACACCCGCCACGGTGTGGCTGTTGAGCCAGGCCGTGTCGTCGGGCAGCCGCCCGTTGAGCACCAGGCCGCCGTCGGCCAGTTCGACCGCCGAGGACAGCAGCGGGTGTCCGGCCGGGCGGGTCCCGGCCCCGGCGGCGAGCCGCTCGCGACGGCCCTGGTCCGGCGTCAGCCAGTAACGCCGCCGCTGGAACGGATAGGTGGGCAGCTCGACCGTACGCGGCCGGTGGCCCTCGCCATCGGCGAACCAGCGGCTCCAGTCGACCGAGAGTCCCGCCGCGAAGCCGTCCCCCAGCGCCCGCGCCACCTGATCCGGGCCGCCCTGGTCCCGGCGCAGCGTGGGCACGGTGGCCGCCGCGACATCGGCCTCCTCGAAGCACTCCCGTAGCGCGGGAATCAGCACCGGGTGGGGGCTGACCTCGATGAACACGCGGTATCCGGCGTCCAGCAGGGTGTCGATCGCCGGGGCGAACCGCACCGGCTGCCGCAGGTTGGTGAACCAGTAGTCGGCGTCCAGCTCCTCCGTCGCCTGCCGGGCGGCGGTGACGGCGGAGTAGAAGGCGGCGCCCGAGGCGCGTGGGGCGAGGTCGGCCAGGTCGGCGCGGATCGTGTCGGCGAGCAGGTCGACCTGCTCGCCGTGCGAGGCGTAGTCCACGTCGATCGTCCGGGCCCGGTGGCCGGCCGCCTCACACGCGGCCACCACCGCGGCGACCTGGTCGGCGGGCCCCGACACCACCGTGGAGTACGGCCCGTTGAACACCGCCACCGTCACCTCGGCGGCCGCCGCGCCCCGGTCCGCGAGGAAGTGCTCGGCCTCCTCGGCGCTCAGCGCGACCGAGGCCATGGTGCCGTGGCCGGCGAGCGCCCGCAGGGCCCGGGAGCGGAGCGCCACCACCCGGGCGCCGTCCCGCAGGGACAGGCCCCCGGCGACGCAGGCGGCGGCGATCTCGCCCTGCGAGTGTCCGACCACCGCGGCGGGCGTCACGCCGAAGCTCTCCCAGACAGCGGCGAGCGACACCATCACCGCCCACAGGCAGGGCTGGAGGACATCGACCCGGGACGGCTCCGGCGCCCCCGGCGCGCCGCGCAGCACGGCGGTGAGCGACCAGTCGACGAACTCGCCGAGCGCCGCCTCGCACTCGGCGACGCGCGCGGCGAAGACGGGGGAGGAGTCAAGCAGTTCCACGCCCATGCCGCGCCACTGCGAACCCTGCCCGGGGAACACCATGACCGGGCCTGGCGCGGCCCCCGCGGAGACGGCGACGGCCGGCGGTCCGTCCGCCACCGCCCGCAGTGCGGACAGCAGCTCGTCCCGGTCCGCGCCGCTGACCACGGCGCGGTGCCGGTCCGCCGACCGCGTGGTGGCAAGCGACCACCCCACGTCCACCGGGGAGGGCGAGGCGCCGTCGGACACCCACTCGGCCAGGCGCCCGGCCTGGGCGCGCAGCGCCTCGGCGGTACGCGCGGACAGCACCCAGGGCACCACGCCACCCTCGGAAGAGGTCTCCGTAGCGGGCGATCCAGGCTGCTCAGGGGCTTGTTCGAGGATGAGGTGGGCGTTGGTGCCGGAGATGCCGAAGGAGGAGACGGCGGCGCGGCGCGGTCGGTCGGTGTCGGGCCACTGGGCGGCCTCGGTGAGGAGGCGGACGTTTCCGGTTGCCCAGTCGACGTGCGGGCTGGGCGCGTCGACATGCAGGGTGGCGGGCAGTCGGCCGTGGCGCAGGGCCATCACCATCTTGATGACGCCCGCGACACCCGCCGCCGCCTGGGTGTGGCCAAGGTTGGACTTGATGGACCCGAGCCACAGGGGCCGGTCCTCGGGGCGGTTCTGTCCGTAGGTGGCGAGGAGGGCCTGGGCCTCGATCGGGTCGCCCAGCGTGGTGCCCGTGCCGTGGGCCTCGACCGCGTCCACGTCCGCCGGGTCCACCCCGGCCGTGGCCAGGGCCTGCCGGATGACGCGCTGCTGCGAGGGGCCGTTGGGGGCGGAGAGGCCGTTGCTCGCGCCGTCCTGGTTCACCGCCGAGCCACGGATGACGGCGAGCACCTGGTGGCCGTTGCGGCGGGCGTCGGAGAGGCGTTCCAGCAGGACGAGCCCGGCACCCTCGCTCCAGCCGGTGCCGTCGGCGGCGGCGGCGAACGGCTTGCACCGCCCGTCCGGGGCCAGGCCGCGCTGCCGGGAGAAGGAGGTGAACATGCCAGGCGTGGCCATCACCGTGGCCCCGCCCGCGAGCGCCAGGTCGCACTCGCCCTGCCGCAGCGCATGCGCGGCGAGGTGCAGCGCCACCAGCGACGACGAACACGCCGTGTCCACCGTGAGCGCCGGTCCCTCCAGGCCGAGGGTGTAGGCCACCCGGCCGGAGGCCACGCTCGGTGTGGAGCCGGTCAGCAGA TGCCCGTCGATCTCGGCGGTGGCCTCGTGCAGCCGTGGCCCGTAGTCCACGGCCGTCGCCCCCACGAAGACGCCGGTCGCGCTGGAGCCCAGCGAGGCCGGGGGGATGCCGGCCCGCTCCAGCAGCTCCCAGGAGAGTTCGAGCAGGATGCGCTGCTGCGGATCCATGGCCAGCGCCTCGCGCGGGCTGATCCCGAAGAACTCCGCGTCGAAGTCCCCCGCGTCGTGGAGAAAGCCGCCCCGGCCCGTCGCGCTGCCGCCGCTGCCGCCGCTGCTGTCCGGGTTTCGGCCGAACAGGTCGGCCAGGTCCCAGCCCCGGTCGCCCGGGAACTCACCCACGGCGTCGGTGCCTTCGTCCACCAGCCGCCACAGTGCCTCGCCGGAGTCGGCCCCGCCCGGGTAGCGGCAGCTGACGGCCACTACGGCGATGGGGTCGTCGGCCGTGGCCGACCGGCGCGTCGGGGCGGGGGCGGTGGCCGGCCGCCCGTCGCGGGCCGCGCGCAGCCAGGCGGCCACGGCGTTCGGCGTGGGGTGGTCGAAGGTGAGGGTGGCGGGCAGGGGGATCCGCGTGGCCGTGCTGAGGCGTTCGCTCAGCTCCGTGGCGGCCAGCGAGTCGAAGCCGAGCTGTTTGAAGGTCAGGCTCGGGTCGATGCCGTCGGGGCTGTCGTGTCCCAGCACCAGGGCCGCGTGCGTACGGACGAGGGCCAGCGGCTCGCCGGGGGAGCCTCCATTGTCGGCGGAGGTGGCGGCCGGGGCGGCGGCCGGGGTGGCGGCCGGGGTGGCGGCCGGGCCCGATGACAGAGCCGGAGCTGCCCGCCGCCCGTCCGCGGCCTCGTCCAGGGCGTCGTTCAAGGCGTCGTCGGGCCAGTGGCGTTCGCGCTGGAAGGCGTAGGTCGGCAGGGGCACCCGCCGGGGCTCGTGCCCGGCGAAGCCCGCCGCCCAGTCGACGGCCACGCCTCGTACGTGCGCCTGTGCCATCGCCGCCACGAAGCTGTGCGCCTCCGGCCGTTCGCGGCGCTGCACCGCCACCGCCGCCGGGCGGGGCGCGTCGCCCGCCCAGGCCGTGGCGCTCTCCCGCGCCATGCCCGTCAGCGAGGCGTCGGGGCCGAGTTCCAGGAAGGTGGTGACCCCCGCGTCGCACAGGTGCCGCACACCGGAGTCGAACCGCACGGCCTGCCGCGCGTGGTCGGCCCAGTAGTCGGGCGAGCGCAGCTGGGCGTCGGTGGCCGGCCGGCCGGTCACGTTCGACACGACGGGGATGGCGGGCGCGGCGAAGGCGAGCCCGGCCGCGACCGCGCGCAGCTCCCCGAGCACGCCGTCCAGGTGCGGGGAGTGGAACGCGTGGCTGACCTTCAGCACGGTGGCCTTGCGGCCCTGCTCCCGCCACGCGGCGGTGGCCGCCACCACGGCGTCGCGGTCGCCGGAGACCACCACGGAGGCGGGTGCGTTGACGGCCGCGAGGCCCACCCGTCCGCCGTGTGCCTCCAGCGCGTGCCCGGCCTCCTCGGCGGTCGCCTGCCAGGCGGCCATCGCCCCCGGGATGGCGACCGACTGCATGAGGCGGCCCCGGGTCGCCACCAGCGCGCAGGCGTCGGGCAGCGAGAACACCCCGGCCACGTGCGCGGCCGTGATCTCGCCGACCGAGTGGCCCATCAGATGGTCGGGCGTGAGGCCCCACGACGTCACCAGGCGGTAGAGGGCCACCTCGAACGCGAACAGCGCGGGCTGGGTGTACTCGGTCCGGTCCAGCGGAGCCTCGGCACCCGGCCTCGCCTCGGCGAACATGACGTCCAGCAGCGGGGTGTCGAGCCAGGGGTCCAGATGCGCGCACACTTCGCGCAGCGCGGCGTCGAAGACCGGGTACGCCGCGCAGAGCTCGTGGCCCATGCCGGGGCGCTGGCTGCCCTGGCCGCTGAACAGGAACGCGACCCGGCCGCCGCCCGCGCTGCCACGGAACACCCCCGTCGCGTCCGCCCCGGCGGCCAGCGCGTCGAGACGACCGAGGAGTTCACCGCCGTCGGTGCCCGTGCCCAGGACGACGGCCCGGTGGGGGAACGCCGTCCGCGTCGTGGCGAGCGCGTGGCCGACGTCCACCGGGTGCAGCCCGGGGTGTGCGCGCAGGTGTTCGCGCAGCGCGGCGGCCTGGGCCGGCAGGGCTTCCGCGCTCGCCGCCGAGACGACCCACGGCGTGACGGGCGGGGCGGGGCGCGCGGACGCGTCCCCGGGCGGTGGCGGCGTCGGCCGCGCCGATGCCGTGGCCGTGGCCGTGGCGGGCGGCTCGGCCAGGACGACATGGCAGTTGGTGCCGCCCATGCCGAAGGAGCTGACCCCGGCGGTGAGCCGCCGCCGCTCGTCCGGCCAGGGCCCGAGCTCGGTCTGGACGCGCAGGCCGAGCTCGTCCAGCGGGATGTCCGGGTTGGGGGTGGCGAAGTTGAGACTCGGCGGAAGCCGCCGGTGATGGATGCTCAGGGCCGTCTTCAGCAGCCCCGCGATGCCCGCGGCGGCCTCCAGGTGGCCCACGTTGGTCTTGACCGAGCCGACCCGCAGCGGGGTGTCCGCCGGGCGCCGCCCGCGGAGCGCGGCGCCGAGCGCGGCGGCCTCGATCGGGTCGCCGACGGGTGTGCCGGTGCCGTGGAGTTCGACGTACTGCGGTTCGGCGGGCGCCACTCCGGCCGCCTGCCACGCCTGTCGCACCACATCCGTCTGCGCCTCGGCGCTCGGCCGGGTCAGCCCGTCGGTGGCGCCGTCGTTGTTGACGGCGCTGCTCTCGATCACGCAGTACACCGGGTCGCCGTCCCTGACGGCCGCGGCCAGCGGTTTGAGGAGCAGCATGCCGCCGCCCTCGCCGCGTACGAAGCCGTTGGCGCGCGCGTCGAAGGTGTAGCAGCGGCCGTCCGGTGAGAGGCCCTGGAACTGGGCCTCGGCCACCCGCATGCTCTCCTCGGCGAGGATCAGGTTCACCCCTCCGGCCAGCGCCAGATCGGCCTCCCCCCGGCGCAGGCTCTCGCAGGCCAGGTGCACGGCGACCAGCCCGGAGGACTGCGCCGCGTCCACCGTGAGACTCGGGCCGCGCAGGCCCAGGCGGTGGGAGATCCGGTTGGCGATGATGCCGCGGTGCAGACCCGTCATCGTGTGACGGGTGATGTCATGGGGCCGGTGCCGGTGGAGGAGCGTGGCGTAGTCGTCCCAGATGGCGCCGGTGAACACGGCGGTGCGGCCGTCCGCCAGGGTGTCGGGGCCGAAACCGGCGTCCTCCATGGCCTCCCAGGCCAGTTCGAGCATCAGCCGCTGCTGTGGATCCATCGCCACGGCCTCGCGCGGCGAGATGCCGAAGAACCGCGCGTCGAAGGTGTCGACGCGCTCCAGGAGCCCGGCGAAGTACGGCGCGGAGCCGGGCCGCGCGGGCAGGCGGCCGGCCGGTACGGGAACGACGGCGCTCCCACCCGTGCGCAGCAGATCCCAGAACGCCTCCGGGTCGGGGGCGTTCGGCAGTCGACAGGCCAGGCCCACGATC GCGACGGGAGACTTCGCCTGCGGTGAGCGGGTCGGGCTGCTCCCGGCCATGAAACCCCCATACCTGAATTGCAAAACCGCTGGTTATGGCTCGCGACATGCCCCGAACGGTGTTCATTCTGGGAATCCGGCCTAAAGTTGATCTAACCGAAAGCACTCCGGGCGGGTTGACAACGGGGTTGACCGCGCCGGGGTAAAGATCGCGTCGATGATCTGCCGCACCGGGGCCCGACACCCGGTGATCGTGGCCCGAGCAACCGGGGCGGCGACGGTCACCGGTCGATGAATCGCCGTATATGCCCATTTGGTTGTCGGTAGATCGACCCTCACACTGTCGCGCCGTGCGCCGTTAGGATGCGGCGATGCTGCTTTCTCTGCGAGCTCTGTTGCCGATTGTTCCGCCGTCAGCGCCAATCGGCGTCTCCGGCGCCGGCCGCAGTGGTGCACGATGATTCAGCGCACCCGCCGATCGCAAAGCGTCCGTCGATCTTCATAGCCGAGAAGCCCACTCTCACCCGACGCCAATATCGAACTGGGGGCAATGTTGTATCCGCGTGTAAAGTGCAGACGTGGCTTCGTTCTGGCTAAGCTGTCCACGGCGTGGGTAAGACGTCACCACTGAGGTCCGAAGCGCTACCATAAAGGCTTCGGGCCGTCTTGTGTATGGTCGACAGGTTCGAACATAAAATCGGGGTGAATTTCGAATGCTGACCAAATTCGAACGCAATGGTGAGTGCCCCAGGGTCGGCATGGCGCCGACCACGGTGGGGGGTCGCGCGTCATGAGGTACGAGATACTCTGCCCGCTGCGCGTCGTGGACGGCACGTCCACGACCTCGCTCAGCGCCCGGAAGATCGAGGTGCTGCTGGCCGCTCTGCTGGTGCGTGCGGACGACGTGGTGAGCAGTGAGGATCTCGTGAAGGAGATCTGGGGTACTCGCGCACCGCGTCAAGCCGTGGCCACGCTCCACGTCTACGTGTCCCAGCTCCGCAAGTTCCTCAGGCGCTCCGCCGACCGGACCGAGAGCCCCATCGTGACCGCTCGCAGCGGCTATGTGCTGCGTCTCGGCGCGGACGAACTGGACTTCCACGTCTTCCAGGACCTGGTGCGCCAGGGCCGGTCCGCGCGGCAGGCCGGGCGCACCCAGGAGGCGTGCGAGGCGTACGAGGAGGGGCTCGACCTCTGGCGCGGCCCGGTGCTCGAGGACCTGCGTGACGGGGCCATCGTCAACGGCTTCGTGTGCTGGGCCGAGCAGGCCCGGCTGGAATGTGTCGAGTCGTTCGTGGACGCCGGCCTGGAAATCGGCCGCCACCGTGAATTCGTCAGCTATCTCTATGCGCGGATCGAGGAGCTTCCGCTGCACGAGGCGTTCTACCGGCAGCTCATGCTGGCGCTCTACCGTTCGGGGTGCAGAGCGGACGCCCTGAGCGTCTATCAGTCGGCGCGGACGGTCCTGGGGGAGGAACTCGGGCTGGAGCCGTCCGCCACTCTCAAGCGGCTGCATCACGCCGTGCTCGTCGATGCGCCGGAGCTCGAAGCCGCCTAGCCGGCGAAGTGGTGCGGAGTAGCGCCCAGGTAAATTCAGTGCCGACCGAGTGCAGGGGGAAATTCAGTGCGGTACGAGATTTTAGGACCTCTCCACGTCGTCGATGAAAAGGGCAGTTCACTGCTCAGAGCGCAGAAACTCGCCACGGTTCTCGCGGTTCTGCTGATCCGCTCGGATCAGGTTGTCGTGGCGGATCAGCTCACGAGGGAGATCTGGGGCGAGCTGCCCCCGCGGCGGACCACCGCGAGCATCTACGTCTGCATTTCGCGGCTCCGCAAGTTCCTGAGCCGGCCCGGCCAGCCGGATCCCATCGTCACGCGTCCCGGTGGATATATGCTGCGCCGGGCGTCGAGCGAATGTGATTTCGATGTCTTCCAGCGTTTGGTCGCGGACGGCCGCAAGTGCCTCAGGGGCGGGGAGCCCGCGGCGGCGGCCACCCGCTTCGAACAGGCCCTGGCGCTGTGGCGGGGCCCGGCGTTCGGCAATGTCCAGCCGGGACCGATCATGGCGGGCTTCCTCAAGCGGCTGGCCGAGATGCGCATCGAGTGCGCGGAGATGTCCGCGGAGGCCCAGCTCGAACTGGGCCGCCACCGGGAGCTGGTCGGCCGGCTGCGGTCCCTCGTCGACGAGTTCCCGCTCCGGGAGACGTTCTACGGCCAGCTGATGCTGGCCCTCTACCGCTCCGACCGGAAGTCCGAGGCGCTCGGCATATACGAGTCCGCCCGGCGCGTCCTCGACCGCGAGCTGGGGCTCGAACCCGGGCGGAAGCTCAGAGAGCTCCGCCACACCGTGCTCGCCTCGACCTGACGGAGCGGGCCGACGGTGACCCGGGCCCCGGCCCCGTCCGTCCGTCTGTCGGTCCGTCTGTCCGGCCGGCCCGAACTCGCCGGGGGCCTCCCGCGCACGGCGCGGGCGAGCCCACCGGCGATGATTCCGGGCCCCGGGTCATTCGGCGACGTAGGTCCGGCGAATCCACTCGAAGACCCGCAGGTGCTGCCCCTGGTGCTCCTCGAACCGAGCTGTGGGCCGGAAGTGTTCGTAGGCGTTGCCGACGAGGAGCTTCAAGGTCGAGTCCGTGTCGTGGACATACCGCGCGCACGACTGATCGGTCAGCTGCGTGGGACCCCCGCGCAGGATGGCATTGGGTGCGGATGCGGACATAAGCGACTGCATTCCCTTCGCTATACATTTCAAGAGAAGGTTGGCATGAGGTGAAAGCGCGCCGATGTGTGACAAACTCCTTGTTCACAAAGGTCTTGTGGGGGCCCGACTTGGGTGAGATGGCAGATCGCCCCGACCTGCTCCCCCGGACCCGCTGCGCCTGGTCCAGCGTAGACGCGCAGGGTCATGCGGTGTGAGGGTCGTTCGACCGACAAAACGACGACGAAACGACCGACAAAGAGGTTCCCTCCCTCACGGATCGTGAGGTTCCCGCCATCGCCGTGTCACACAGACGGTCGCCGAGGATGGATGACTCATGCCGCTTCCCTCACTTCCGCTCCGCGTCCAGCTGCCGGGCCTGAGGCGCTTACCCGAGTCATCCGGGCCCTGGGCGCGCGAATACGTGGCGTTCAACCGGCAGTTGGCGCTCTACGGCCGCGCCGGGGTGATGAGCGCCTGCGCGGTGCTGGCCGTGGTGGGCATGCCGGCGGGGAAGATGCCGGTCGCGGCGGCGATCGCGTCCGCCGCGTTCGCCTGGAGCTGGATCCACCTGTGGCTCGCCAACGCCTCGACGTTACGGCCGGGGCGGCTGCTCGCCCTCGACCTGGCGGTCATGACCGCGTTGTGTCTCAGCCAGCGGCTCACCGTCCCCGACGCGCCGACCGGCCACGGCAGCACCTGGATCCTGGTGTCCCTCAACTTCACGGCCGTGGCCTACCAGCTCATGCAGCCGCCCCTCGTCGCGATTCTGGCGACGCTCTGGCTGTGCTGCGCGGACCTGGCGGGCGCCGCGCTGCACCCGCGCCAGGAGTTGACCGACGTCGC GTACAGCCTGGGCTGGGTGCTGGTGAACATCGTCCTGGCCCAGGCCGTGGTCCGACTGGTGTTCGGCGAGAGCCGGACGGCCGACGAGGCCGCCGACCGGGCCGCGCGGGCGCGCCGGCAGGGCGAGGCGGCGGAGGCCCGGTGCGCGGCGGAGCGGGAACACCTGGCCGCGCTCCACGACACCGCCTGCGCGACCCTGCTGATCGCCTCGGCGCCATGGGCGTCGATGCGGCCGGAGACGCTGCGCGCCCAGGCCGCGAGCGACCTCGTCCGACTGCGCACCGAGCAGTCCGCCGCGGGGGAGGTGGACCTGACCGTCGAGCTGGCCGCCGAGATCGCCGCGCATCCCCTCCGGGCCACCACGCTGTTCGACCCGGATCTCGGCACGGTCTGGCGCCCCGCCGCGGCGGCGCTGCGCGGCGGCCTCGGCGAGGCGCTGCGCAACGTGGAGCGCTACGCGGGGGTGGGCGCCGTGCGGGTCACGGCGGTGCGCACCCGCCACACGGTGGTCGTGACCATCACGGACAGCGGGGTGGGGTTCGACACCGGGCGCGTTCCCGCCCAGCGGATCGGGATCCAGCGTTCCATCCGCGGGCGGATGGCGAGCGTGGGGGGCCGCGGCACCGTGGAGTCCCGGCCGGGGTGCGGCACGACGGTACGTCTGGAGTGGCCGCGTGCCTGAGGACCTGCGTCAGCTGATCACCCGTCACCAGCTCCGCGGCATCCGCGCCGGGGCACTGGTCATCCTGGTCACCCTCCACTTCGCACTCGCGCTGCCCGGCCTCCTGAGCAGCCTGGAGGAGTACCGGCACCCCTGGGTCCCCGTGACCGCCTACGGACTGCTCACCGTGATTCTCGGCGGCAGCGTCCTTCTCGGCCTCGACGGGCGCCCCTGGCCGCGCGGCTGGGTGCCGACGGCGCTGGCCGGCGCCTTCGCCGCCTCGGTGGCCGCCACCACCCAGGTGCCGGCGAGCCAGTACTTCGTCGGCGCCTCCCACTGGTCCTTCACCCTCGTGGGCTGGTTCGCCGTCGTCCTGCTCGGCCACCGGGGACCCATGGCGTTCGGCGCCTTCATCGGCTGCCATCTGGCCGTCAACACCGCCCAGTTGCTGACGGCCGACGTCCCCTCGCGGCCCATCGCCGCGGGCATGGCCGCGAGCGCCCTGTCCGCCTGCTGCTTCCAGATGACCATCGCCGCCGGCGCGAAGCTGCTGCTCAACAGCAGCACCGCCATCGGGGAGGCCCTCCAGGTGCAGGAGCGGGTGCGCACCCGCATCGCCGTCGCCACCCAGATCCAGACGGACCAGCGCCGGCGCTACGCCGAGCTGAACGCGACCGTGCTGCCCCTGCTCACCGGCCTCGCCAACGGCTCCCTGGACCCACGGGACGACGAGGTGCGGCGGGCCTGCGCGCTGGAAGCCGCCCGGCTGAGGCGGCTGTTCGCGGAGAGCGACTGTACGTTCGACCCGCTGGTCCACGAGATGCGGGCCTGTATCGACGTCGCCGAGCGCAACGGGACCACCGTGCAGCTCGCCGTCCGCGGCGACCCCCTCGAACTGCCGCTGCCGCTGCGCCGCGCGCTCCTGGACCCGGTCATCGCCGCACTGGCCGCCGCCCGGCAGACGGCGCGCGTCACCGTGGTGCGCGGCGAGAATCAGGTACGCGTGGGCGTGGTGGTGGACGCGCTGCGGGATCGGCTGCCCGAGCCGGCCGCGAGCGGGATCCGGGTGCGCACGGTCCGCGCGGAGGACCGTCTCCTTGTGGAGGCCGCGTGCCGGATCACAGCTCGTCCAGCGCGATCAGACCGTCCTGAACCGCCCTGGCCACCAGCTTGGCCTTCGTGGTCGCCGGGCGGCCGGCGTTCGCGTACTTGATCCGCACCCGGTCCAGATAGGTGTTCACCGTGCGCTCCGAGATCCCCAGGCTCTTGGCCACCATGGCCTTCGACTCGGACTGGAACCACTCCACCAGCACCTCCACCTCCCGCACGGACAGCACCGGGCGGCTCTGCCGGGTGTCGCTGCCGAACGCGCCGGCCAGCGACGGGGGTGTGTAGGGGATGTCCGCGGCGGCGGCCTGGATCGCCTTGACCAGGTGCCGCTGCCCCTCCGCCTTGGTGATGTAGGTGGCCGAACCCAGGTCCATGCAGGTCAACGCCGCCTCCTGGCTGTCCCGCATCGTGTAGACGACGACCTTGCGCTCCTGCGCGACCAGCACCTGCAACTCCTCGTACGCGGGTCGCCCGTTCTGCAACAGCAGGTCCATGACCACGACGTCCGCGCCCCGGCCCGGACCGCAGAGGGCCACCGAAAGCTCCGACCCCTCGGCCACCACCTTGATCGGCACGTGGGCGGCGGCACACCAGGCCGCGATGCCCGCGCGTACCACGGGGTGATCATCGACCAACACGACGCTGAGGGCCAT(SEQ ID NO：35)
the explanation of the biosynthetic gene cluster is given by taking the biosynthetic gene cluster of the streptomyces as an example, and the biosynthetic gene cluster comprises a linear polyketide synthase gene module I, a glycosyl synthesis related gene module and a modifying gene module. The modules of the biosynthetic gene cluster of Streptomyces are described in detail below, specifically as follows:
wherein, the I type linear polyketide synthase gene module comprises 11 genes of 2A1, 2A2, 2A3, 2A4, 2A5, 2A6, 2A7, 2A8, 2A9, 2A10 and 2A11, and the specific details are as follows:
according to an embodiment of the invention, the 2a1 gene encodes SEQ ID NO: 1. The method comprises the following specific steps:
Figure BDA0000889457240000331
Figure BDA0000889457240000341
according to the embodiment of the invention, the 2A1 gene is located at 92698-101334 base pairs of the gene cluster nucleotide sequence of the streptomyces, is 8637 base pairs in length, encodes polyketide synthetase and is 2878 amino acids in length.
According to an embodiment of the invention, the 2a2 gene encodes SEQ ID NO: 2, specifically the amino acid sequence shown in the specification as follows:
Figure BDA0000889457240000342
Figure BDA0000889457240000351
according to the embodiment of the invention, the 2A2 gene is located at 85970-92683 base of the gene cluster sequence of the streptomyces, has the length of 6714 base pairs, encodes polyketide synthase and has the length of 2237 amino acids.
According to an embodiment of the invention, the 2a3 gene encodes SEQ ID NO:3, specifically the following amino acid sequence:
Figure BDA0000889457240000352
Figure BDA0000889457240000361
Figure BDA0000889457240000371
according to the embodiment of the invention, the 2A3 gene is located at 73776-85781 bases of the gene cluster nucleotide sequence of the streptomyces, is 12006 base pairs in length, encodes polyketide synthase and is 4001 amino acids in length.
According to an embodiment of the invention, the 2a4 gene encodes SEQ ID NO: 4, specifically the following amino acid sequence:
Figure BDA0000889457240000372
Figure BDA0000889457240000381
according to the embodiment of the invention, the 2A4 gene is located at 62077-73755 bases of the gene cluster nucleotide sequence of the streptomyces, has the length of 11679 base pairs, encodes polyketide synthase and has the length of 3892 amino acids.
According to an embodiment of the invention, the 2a5 gene encodes SEQ ID NO: 5, specifically the following amino acid sequence:
Figure BDA0000889457240000382
Figure BDA0000889457240000391
Figure BDA0000889457240000401
according to the embodiment of the invention, the 2A5 gene cluster is located at 50179-62031 bases of the gene cluster nucleotide sequence of the streptomyces, has the length of 11853 base pairs, encodes polyketide synthase and has the length of 3950 amino acids.
According to an embodiment of the invention, the 2a6 gene encodes SEQ ID NO: 6, specifically the following amino acid sequence:
Figure BDA0000889457240000402
according to the embodiment of the invention, the 2A6 gene is located at 45212-50158 bases of the gene cluster nucleotide sequence of the streptomyces, has the length of 4947 base pairs, encodes polyketide synthase and has the length of 1648 amino acids.
According to an embodiment of the invention, the 2a7 gene encodes SEQ ID NO: 7, specifically the following amino acid sequence:
Figure BDA0000889457240000403
Figure BDA0000889457240000411
according to the embodiment of the invention, the 2A7 gene is located at 33430-38406 bases of the gene cluster nucleotide sequence of the streptomyces, is 4977 base pairs in length, encodes polyketide synthase, and is 1658 amino acids in length.
According to an embodiment of the invention, the 2A8 gene encodes SEQ ID NO: 8, specifically the following amino acid sequence:
Figure BDA0000889457240000412
Figure BDA0000889457240000421
Figure BDA0000889457240000431
according to the embodiment of the invention, the 2A8 gene is located at 7986-19214 th base of the gene cluster nucleotide sequence of the streptomyces, is 11229 base pairs in length, encodes polyketide synthase and is 3742 amino acids in length.
According to an embodiment of the invention, the 2a9 gene encodes SEQ ID NO: 9, specifically the following amino acid sequence:
Figure BDA0000889457240000432
according to the embodiment of the invention, the 2A9 gene is located at 25758-28115 bases of the gene cluster nucleotide sequence of the streptomyces, has the length of 2358 base pairs, encodes polyketide synthase and has the length of 785 amino acids.
According to an embodiment of the invention, the 2a10 gene encodes SEQ ID NO: 10, specifically as follows:
MEPIEESTGD SRLAERLATA TPEEREQLLR GLVLRHAAEI LEAPELDEES NFVENGLTSL 60
RALQLSKALM NDTGLEAPLV AIVEHPTPTL LGKFLSETYE ADAA (SEQ ID NO：10)
according to the embodiment of the invention, the 2A10 gene is located at 32185-32499 bases of the gene cluster nucleotide sequence of the streptomyces and has a length of 315 base pairs, and the ACP protein which codes polyketide synthase has a length of 104 amino acids.
According to an embodiment of the invention, the 2a11 gene encodes SEQ ID NO: 11, specifically the amino acid sequence shown in the specification as follows:
Figure BDA0000889457240000433
Figure BDA0000889457240000441
according to the embodiment of the invention, the 2A11 gene is located at 19227-25730 bases of the gene cluster nucleotide sequence of the streptomyces, has the length of 6504 base pairs, encodes polyketide synthase and has the length of 2167 amino acids.
The glycosyl synthesis related gene module comprises 9 genes including 2G1, 2G2, 2G3, 2G4, 2G5, 2G6, 2G7, 2G8 and 2M, and the specific steps are as follows:
according to an embodiment of the invention, the 2G1 gene encodes SEQ ID NO: 12, specifically as follows:
Figure BDA0000889457240000442
according to the embodiment of the invention, the 2G1 gene is located at 40030-: TTP thymidine transferase, 270 amino acids in length.
According to an embodiment of the invention, the 2G2 gene encodes SEQ ID NO: 13, specifically as follows:
Figure BDA0000889457240000443
according to the embodiment of the invention, the 2G2 gene is located at 40839-41831 bp of the gene cluster nucleotide sequence of Streptomyces, is 993 bp in length, encodes dTDP glucose-4, 6-dehydratase, and is 330 amino acids in length.
According to an embodiment of the invention, the 2G3 gene encodes SEQ ID NO: 14, specifically as follows:
Figure BDA0000889457240000444
according to the embodiment of the present invention, the 2G3 gene is located at 41895-43199 bases of the cluster nucleotide sequence of the Streptomyces gene, is 1305 base pairs in length, encodes NDP-D-glucose-3, 4-dehydratase, and is 434 amino acids in length.
According to an embodiment of the invention, the 2G4 gene encodes SEQ ID NO: 15, specifically as follows:
Figure BDA0000889457240000451
according to the embodiment of the invention, the 2G4 gene is located at 43196-44194 bases of the gene cluster nucleotide sequence of Streptomyces, has a length of 999 base pairs, and encodes NDP-D-glucose-4, 6-dehydratase, NDP-D-glucose-4-isomerase and NDP-D-glucose-4-reductase with a length of 332 amino acids.
According to an embodiment of the invention, the 2G5 gene encodes SEQ ID NO: 16, specifically as follows:
Figure BDA0000889457240000452
according to the embodiment of the invention, the 2G5 gene is located at 38479-3975 bases of the gene cluster nucleotide sequence of Streptomyces, has a length of 1272 base pairs, encodes glycosyltransferase, and has a length of 423 amino acids.
According to an embodiment of the invention, the 2G6 gene encodes SEQ ID NO: 17, specifically as follows:
Figure BDA0000889457240000453
according to the embodiment of the present invention, the 2G6 gene is located at 3445-4629 bases of the gene cluster nucleotide sequence of Streptomyces, is 1185 base pairs in length, encodes glycosyltransferase, and is 394 amino acids in length.
According to an embodiment of the invention, the 2G7 gene encodes SEQ ID NO: 18, specifically as follows:
Figure BDA0000889457240000454
according to the embodiment of the present invention, the 2G7 gene is located at 1169-2848 bases of the gene cluster nucleotide sequence of Streptomyces, is 1680 base pairs in length, encodes glycosyltransferase, and is 559 amino acids in length.
According to an embodiment of the invention, the 2G8 gene encodes SEQ ID NO: 19, specifically the amino acid sequence shown in the specification as follows:
Figure BDA0000889457240000461
according to the embodiment of the invention, the 2G8 gene is located at the 1 st to 1116 th bases of the gene cluster nucleotide sequence of the streptomycete, is 1116 base pairs in length, encodes endoglucanase and is 371 amino acids in length.
According to an embodiment of the invention, the 2M gene encodes SEQ ID NO: 20, specifically the following amino acid sequence:
Figure BDA0000889457240000462
according to the example of the present invention, the 2M gene is located at 44191-45117 bases of the gene cluster nucleotide sequence of Streptomyces, 927 base pairs in length, encodes methyltransferase, and is 308 amino acids in length.
Wherein, the modifying genes comprise 5 genes of 2O, 2I, 2E, 2P1 and 2P2, and the specific steps are as follows:
according to an embodiment of the invention, the 2O gene encodes SEQ ID NO: 21, specifically as follows:
Figure BDA0000889457240000463
according to the embodiment of the invention, the 2O gene is located at 30711-32147 th base of the gene cluster nucleotide sequence of the streptomyces, is 1437 base pairs in length, encodes cyclooxygenase and is 478 amino acids in length.
According to an embodiment of the invention, the 2I gene encodes SEQ ID NO: 22, specifically as follows:
Figure BDA0000889457240000464
according to the embodiment of the invention, the 2I gene is positioned at 29661-30569 bases of the gene cluster nucleotide sequence of the streptomyces, has a length of 909 base pairs, encodes ketosteroid isomerase and has a length of 302 amino acids.
According to an embodiment of the invention, the 2E gene encodes SEQ ID NO: 23, specifically as follows:
Figure BDA0000889457240000465
according to the embodiment of the invention, the 2E gene is positioned at 32547-33428 base of the gene cluster nucleotide sequence of the streptomycete, has the length of 882 base pairs, encodes epoxide hydrolase and has the length of 293 amino acids.
According to an embodiment of the invention, the 2P1 gene has the sequence of SEQ ID NO: 24, specifically as follows:
Figure BDA0000889457240000471
according to the embodiment of the invention, the 2P1 gene is located at 6809-7960 bases of the amino acid sequence of the streptomyces gene cluster, is 1152 base pairs in length, encodes cytochrome P450 protein and is 383 amino acids in length.
According to an embodiment of the invention, the 2P2 gene encodes SEQ ID NO: 25, specifically as follows:
Figure BDA0000889457240000472
according to the embodiment of the invention, the 2P2 gene is located at the 28182-29462 bp position of the gene cluster nucleotide sequence of the streptomyces, has the length of 1281 base pairs, encodes cytochrome P450 protein and has the length of 426 amino acids.
According to an embodiment of the present invention, the biosynthetic gene cluster further comprises: a regulatory gene module comprising: 2R1 gene, 2R2 gene, 2R3 gene, 2R4 gene, 2T1 gene, 2T2 gene, 2T3 gene, 2T4 gene and 2T5 gene, wherein the sequence information of the genes is as follows:
according to an embodiment of the invention, the 2R1 gene encodes SEQ ID NO: 26, specifically as follows:
Figure BDA0000889457240000473
according to the embodiment of the invention, the 2R1 gene is located at 103016 and 103636 bases of the cluster nucleotide sequence of the streptomyces gene, has a length of 621 base pairs, encodes a regulatory protein, and has a length of 206 amino acids.
According to an embodiment of the invention, the 2R2 gene encodes SEQ ID NO: 27, specifically as follows:
Figure BDA0000889457240000474
according to the embodiment of the invention, the 2R2 gene is located at 102065-102829 base of the gene cluster nucleotide sequence of the streptomyces, is 765 base pairs in length, encodes a regulatory protein and is 254 amino acids in length.
According to an embodiment of the invention, the 2R3 gene encodes SEQ ID NO: 28, specifically as follows:
Figure BDA0000889457240000475
according to the embodiment of the invention, the 2R3 gene is located at the 5548-6558 th base of the gene cluster nucleotide sequence of the streptomyces, has the length of 1011 base pairs, encodes a transcription regulatory factor and has the length of 336 amino acids
According to an embodiment of the invention, the 2R4 gene encodes SEQ ID NO: 29, specifically as follows:
Figure BDA0000889457240000481
according to the embodiment of the invention, the 2R4 gene is located at 4589-5539 bases of the gene cluster nucleotide sequence of Streptomyces, has a length of 951 base pairs, encodes a transcription regulatory factor, and has a length of 316 amino acids.
According to an embodiment of the invention, the 2T1 gene encodes SEQ ID NO: 30, specifically as follows:
Figure BDA0000889457240000482
according to an embodiment of the invention, the 2T1 gene is located in the Streptomyces strain SEQ ID NO: 35, the length of the gene sequence fragment is 1428 base pairs, and the gene sequence fragment encodes membrane integrated transfer protein, and has the length of 475 amino acids.
According to an embodiment of the invention, the 2T2 gene has the sequence of SEQ ID NO: 31, specifically as follows:
Figure BDA0000889457240000483
according to an embodiment of the invention, the 2T2 gene is located in the Streptomyces strain SEQ ID NO: 35, has the nucleotide sequence shown in SEQ ID NO:36, has the length of 687 base pairs, codes ABC transfer factor and has the length of 228 amino acids.
According to an embodiment of the invention, the 2T3 gene encodes SEQ ID NO: 32, specifically as follows:
Figure BDA0000889457240000484
according to the embodiment of the invention, the 2T3 gene is located at 106595-107239 bases of the gene cluster nucleotide sequence of Streptomyces, has a length of 645 base pairs, encodes a two-component feedback regulator, and has a length of 214 amino acids.
According to an embodiment of the invention, the 2T4 gene encodes SEQ ID NO: 33, specifically as follows:
Figure BDA0000889457240000485
according to the embodiment of the invention, the 2T4 gene is located at 105490-106689 bases of the gene cluster nucleotide sequence of Streptomyces, is 1200 base pairs long, encodes a chemoreceptor, and is 399 amino acids long.
According to an embodiment of the invention, the 2T5 gene encodes SEQ ID NO: 34, specifically as follows:
Figure BDA0000889457240000491
according to the embodiment of the invention, the 2T5 gene is located at 104394-105497 bp of the gene cluster nucleotide sequence of Streptomyces, is 1104 bp in length, encodes two-component sensor histidine kinase, and is 367 amino acids in length.
Referring to fig. 1, according to an embodiment of the present invention, along the 5 '-3' end of the gene, 2T2 gene, 2T1 gene, 2G8 gene, 2G7 gene, 2G6 gene, 2R4 gene, 2R3 gene, 2P1 gene, 2a8 gene, 2a11 gene, 2A9 gene, 2P2 gene, 2I gene, 2O gene, 2a10 gene, 2E gene, 2A7 gene, 2G5 gene, 2G1 gene, 2G2 gene, 2G3 gene, 2G4 gene, 2M gene, 2A6 gene, 2a5 gene, 2A4 gene, 2A3 gene, 2a 2a1 gene, 2R2 gene, 2R1 gene, 2T5 gene, 2T4 gene, and 2T3 gene are sequentially connected in series.
In the present invention, "tandem" merely indicates the order of connection of genes, and adjacent genes may be directly connected to each other, or other gene fragments may be present or partial sequences at the connection may overlap each other.
According to some embodiments of the invention, the 2T2 gene, the 2G7 gene, the 2R4 gene, the 2R3 gene, the 2P2 gene, the 2G5 gene, the 2G3 gene, the 2G4 gene, the 2M gene, the 2a6 gene, the 2a5 gene, the 2a4 gene, the 2A3 gene, the 2a2 gene, the 2a1 gene, the 2T5 gene, and the 2T3 gene are expressed in the same direction.
According to some embodiments of the invention, the 2T1 gene, the 2G8 gene, the 2G6 gene, the 2P1 gene, the 2A8 gene, the 2a11 gene, the 2a9 gene, the 2I gene, the 2O gene, the 2a10 gene, the 2E gene, the 2a7 gene, the 2G1 gene, the 2G2 gene, the 2R2 gene, the 2R1 gene, and the 2T4 gene are expressed in the same direction.
Microorganisms
According to another aspect of the present invention, there is provided a microorganism. According to an embodiment of the present invention, the microorganism has the aforementioned biosynthetic gene cluster. The inventors have surprisingly found that the J1001-2 compound can be synthesized efficiently, simply and at low cost by performing a fermentation treatment using the microorganism.
According to an embodiment of the invention, the microorganism is streptomyces. According to a particular embodiment of the invention, the microorganism is Streptomyces hygroscopicus subtype Streptomyces.
According to yet another aspect of the present invention, there is provided a process for preparing a J1001-2 compound. Referring to fig. 2, the method is explained according to an embodiment of the present invention, the method including:
s100 fermentation treatment
According to an embodiment of the present invention, the fermentation treatment is performed using the aforementioned microorganism to obtain a fermented microorganism.
According to a specific embodiment of the invention, the fermentation medium conditions are: soluble starch (30g/L), soybean cake powder (10g/L), yeast extract (2.5g/L) and calcium carbonate (3 g/L). Therefore, the fermentation effect is good, and the yield of the J1001-2 compound is high.
According to a particular embodiment of the invention, the fermentation treatment is carried out for a period of 7 to 8 days.
S200 wall breaking treatment
According to the embodiment of the invention, the fermented microorganism is subjected to wall breaking treatment to obtain a solution containing the J1001-2 compound.
S300 purification
According to the embodiment of the invention, the J1001-2 compound is purified from the solution containing the J1001-2 compound, and the J1001-2 compound is obtained as a product.
According to some embodiments of the invention, the solution containing the J1001-2 compound is spin dried, extracted with ethyl acetate, concentrated, and then separated by silica gel column and further purified by semi-preparative HPLC to give the desired product.
According to the method for producing the J1001-2 compound of the embodiment of the present invention, the J1001-2 compound can be efficiently synthesized by performing the fermentation treatment using the aforementioned microorganism. Then, the pure J1001-2 compound can be obtained efficiently, simply and at low cost through microbial wall breaking treatment and separation and purification treatment.
According to some embodiments of the invention, the biosynthetic pathway of the J1001-2 compound is predicted by genetic prediction as shown in fig. 3. On one hand, long-chain fatty acid chains are finally formed by catalyzing the extension synthesis of propionyl coenzyme A and methylmalonyl coenzyme A by a polyketide synthesis gene cluster, the intermediate products of J1001-2 are obtained by modifying 2O, 2I and 2E genes, on the other hand, glycosyl is obtained by catalyzing genes such as 2G1, 2G2, 2G3, 2G4 and 2M, glycosyl is transferred onto the fatty acid chains by glycosyltransferases 2G5, 2G6 and 2G7, and finally J1001-2 is formed by the action of P450 enzymes 2P1 and 2P 2.
The present invention is described below with reference to specific examples, which are intended to be illustrative only and are not to be construed as limiting the invention.
The scheme of the invention will be explained with reference to the following examples. It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention. The examples do not specify particular techniques or conditions, and are carried out according to techniques or conditions described in literature in the art (for example, refer to molecular cloning, a laboratory Manual, third edition, scientific Press, written by J. SammBruke et al, Huang Petang et al) or according to product instructions. The reagents or apparatus used are not indicated by the manufacturer, but are conventional products available commercially, for example from Illumina.
Example 1
Streptomyces hygroscopicus subtype Streptomyces is inoculated to an SFM plate and cultured for about three days to four days. When colonies are formed in the SFM culture medium, selecting single colonies to a seed culture medium for culture for about three days to four days, inoculating the single colonies to a fermentation culture medium according to the amount of 1 percent (the components of the fermentation culture medium are 30g/L of soluble starch, 10g/L of soybean cake powder, 2.5g/L of yeast extract, CaCO 3 3g/L, pH 7.2). The culture conditions are as follows: 30 ℃ and 220 rpm, and culturing for 7 to 8 days.
And when the culture is carried out for 7 th or 8 th day, collecting fermentation liquor, separating the supernatant from the mycelium by high-speed centrifugation, adding the same amount of acetone into the mycelium to break the bacteria, carrying out ultrasonic treatment for 20min, carrying out rotary evaporation on the acetone, extracting the supernatant and the mycelium by using the same amount of ethyl acetate, and repeating the steps once. Concentrating to dryness by rotary evaporation, then carrying out crude separation by using a silica gel column to obtain a crude product detected by using HPLC, and considering that the HPLC result shows that the yield of the bacterium is higher, carrying out TLC (thin layer chromatography) plate spotting on a concentrated sample, developing in an iodine cylinder, removing a very obvious spot, dissolving by using methanol, carrying out high-speed centrifugation, taking a supernatant as a mass spectrum, and determining the position of a target point, wherein the result is shown in figure 4, and the compound is a J1001-2 compound.
Example 2
The sequence information of the biosynthetic gene cluster of the J1001-2 compound is researched by taking Streptomyces hygroscopicus subtype Streptomyces as a research object, and the specific method is as follows:
1. extraction of DNA
(1) Streptomyces hygroscopicus subtype Streptomyces is inoculated into TSBY culture medium for 2 days, and after the culture, the strain is collected at 7000rpm × 5 min.
(2) The collected cells were discarded from the supernatant, and 30ml of SET buffer was added thereto, and the mixture was dispersed and centrifuged at 7000rpm for 5 minutes. The supernatant was discarded again, 10ml of SET buffer was added, the cells were disrupted, 300. mu.l of lysozyme (100mg/ml) was added, and the mixture was subjected to 37 ℃ water bath for 30 to 60 minutes, 100. mu.l of proteinase K (100mg/ml), 10. mu.l of RNase (10mg/ml) and 37 ℃ water bath for 30 minutes.
(3) To the mixture obtained in step (2) was added 1.2ml of 10% SDS, and the mixture was incubated at 55 ℃ for 2 hours while shaking gently every 15 minutes until the liquid became clear.
(4) 4ml of 5M NaCl was added to the liquid obtained in step (3), mixed well and cooled to 37 ℃. 10ml of chloroform was added, gently mixed for 30 minutes, and centrifuged at 12000rpm for 15 minutes.
(5) And (4) adding 10ml of chloroform into the centrifuged supernatant in the step (4), gently mixing for 30 minutes, and centrifuging at 12000rpm for 15 minutes.
(6) And (4) taking the supernatant centrifuged in the step (5), adding 0.8-time volume of isopropanol, and gently swirling until filamentous DNA appears.
(7) And (3) picking out the DNA in an EP tube, adding 70% ethanol for washing, then pouring out the ethanol, naturally drying, dissolving in a certain amount of double distilled water, and extracting to obtain the DNA of the streptomycete.
2. DNA sequence analysis
The genome was sent to a sequencer to perform genome sequencing, thereby obtaining genome information, and the assembled genome was predicted for a gene encoding a protein using GeneMarkS. Then, an anti SMASH software is utilized to predict a region similar to a J1-001 compound synthetic gene cluster, and a J1001-2 synthetic related gene is excavated by combining the comparison result of proteins in the J1-001 compound synthetic gene cluster in the bacterial coding gene data set. The structure of the gene cluster is shown in FIG. 2, and is responsible for the biosynthesis of compound J1001-2, and the functional analysis of each gene is shown in Table 1.
Figure BDA0000889457240000501
Figure BDA0000889457240000511
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
While embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.
Figure IDA0000923029300000011
Figure IDA0000923029300000021
Figure IDA0000923029300000031
Figure IDA0000923029300000041
Figure IDA0000923029300000051
Figure IDA0000923029300000061
Figure IDA0000923029300000071
Figure IDA0000923029300000081
Figure IDA0000923029300000091
Figure IDA0000923029300000101
Figure IDA0000923029300000111
Figure IDA0000923029300000121
Figure IDA0000923029300000131
Figure IDA0000923029300000141
Figure IDA0000923029300000151
Figure IDA0000923029300000161
Figure IDA0000923029300000171
Figure IDA0000923029300000181
Figure IDA0000923029300000191
Figure IDA0000923029300000201
Figure IDA0000923029300000211
Figure IDA0000923029300000221
Figure IDA0000923029300000231
Figure IDA0000923029300000241
Figure IDA0000923029300000251
Figure IDA0000923029300000261
Figure IDA0000923029300000271
Figure IDA0000923029300000281
Figure IDA0000923029300000291
Figure IDA0000923029300000301
Figure IDA0000923029300000311
Figure IDA0000923029300000321
Figure IDA0000923029300000331
Figure IDA0000923029300000341
Figure IDA0000923029300000351
Figure IDA0000923029300000361
Figure IDA0000923029300000371
Figure IDA0000923029300000381
Figure IDA0000923029300000391
Figure IDA0000923029300000401
Figure IDA0000923029300000411
Figure IDA0000923029300000421
Figure IDA0000923029300000431
Figure IDA0000923029300000441
Figure IDA0000923029300000451
Figure IDA0000923029300000461
Figure IDA0000923029300000471
Figure IDA0000923029300000481
Figure IDA0000923029300000491
Figure IDA0000923029300000501
Figure IDA0000923029300000511
Figure IDA0000923029300000521
Figure IDA0000923029300000531
Figure IDA0000923029300000541
Figure IDA0000923029300000551
Figure IDA0000923029300000561
Figure IDA0000923029300000571
Figure IDA0000923029300000581
Figure IDA0000923029300000591
Figure IDA0000923029300000601
Figure IDA0000923029300000611
Figure IDA0000923029300000621

Claims (4)

1. A biosynthetic gene cluster of a J1001-2 compound, wherein the structure of the J1001-2 compound is as follows,
Figure FDA0003499977930000011
wherein the biosynthetic gene cluster comprises:
the I type linear polyketide synthase gene module comprises: 2a1 gene, 2a2 gene, 2A3 gene, 2a4 gene, 2a5 gene, 2a6 gene, 2a7 gene, 2A8 gene, 2a9 gene, 2a10 gene and 2a11 gene;
a glycosyl synthesis-associated gene module comprising: 2G1 gene, 2G2 gene, 2G3 gene, 2G4 gene, 2G5 gene, 2G6 gene, 2G7 gene, 2G8 gene and 2M gene; and
a modifier gene module comprising: 2O gene, 2I gene, 2E gene, 2P1 gene, and 2P2 gene;
wherein the content of the first and second substances,
the 2A1 gene encodes SEQ ID NO: 1;
the 2A2 gene encodes SEQ ID NO: 2;
the 2A3 gene encodes SEQ ID NO: 3;
the 2A4 gene encodes SEQ ID NO: 4;
the 2A5 gene encodes SEQ ID NO: 5;
the 2A6 gene encodes SEQ ID NO: 6;
the 2A7 gene encodes SEQ ID NO: 7;
the 2A8 gene encodes SEQ ID NO: 8;
the 2A9 gene encodes SEQ ID NO: 9;
the 2A10 gene encodes SEQ ID NO: 10;
the 2A11 gene encodes SEQ ID NO: 11;
the 2G1 gene encodes SEQ ID NO: 12;
the 2G2 gene encodes SEQ ID NO: 13;
the 2G3 gene encodes SEQ ID NO: 14;
the 2G4 gene encodes SEQ ID NO: 15;
the 2G5 gene encodes SEQ ID NO: 16;
the 2G6 gene encodes SEQ ID NO: 17;
the 2G7 gene encodes SEQ ID NO: 18;
the 2G8 gene encodes SEQ ID NO: 19;
the 2M gene encodes SEQ ID NO: 20;
the 2O gene encodes SEQ ID NO: 21;
the 2I gene encodes SEQ ID NO: 22;
the 2E gene encodes SEQ ID NO: 23;
the 2P1 gene encodes SEQ ID NO: 24;
the 2P2 gene encodes SEQ ID NO: 25;
the biosynthetic gene cluster further comprises:
a regulatory gene module comprising: 2R1 gene, 2R2 gene, 2R3 gene, 2R4 gene, 2T1 gene, 2T2 gene, 2T3 gene, 2T4 gene and 2T5 gene, wherein
The 2R1 gene encodes SEQ ID NO: 26;
the 2R2 gene encodes SEQ ID NO: 27, or a pharmaceutically acceptable salt thereof;
the 2R3 gene encodes SEQ ID NO: 28;
the 2R4 gene encodes SEQ ID NO: 29;
the 2T1 gene encodes SEQ ID NO: 30;
the 2T2 gene encodes SEQ ID NO: 31;
the 2T3 gene encodes SEQ ID NO: 32;
the 2T4 gene encodes SEQ ID NO: 33;
the 2T5 gene encodes SEQ ID NO: 34;
wherein, along the 5 '-3' end of the gene, said 2T2 gene, said 2T1 gene, said 2G8 gene, said 2G7 gene, said 2G6 gene, said 2R4 gene, said 2R3 gene, said 2P1 gene, said 2A8 gene, said 2A11 gene, said 2P 11 gene, said 2I gene, said 2O gene, said 2A11 gene, said 2E gene, said 2A11 gene, said 2G 11 gene, said 2M gene, said 2A11 gene, said 2R 11 gene, said 2T 11 gene, said 2G 11 gene, said 2A11 gene and said 2A11 gene in series;
the nucleotide sequence of the biosynthesis gene cluster is shown as SEQ ID NO: shown at 35.
2. A Streptomyces which is a Streptomyces hygroscopicus subtype Streptomyces and has the biosynthetic gene cluster according to claim 1.
3. A method of preparing a J1001-2 compound, comprising:
performing a fermentation treatment using the streptomyces of claim 2 to obtain fermented streptomyces;
performing wall breaking treatment on the fermented streptomyces so as to obtain a solution containing the J1001-2 compound; and
purifying the J1001-2 compound from the solution containing the J1001-2 compound so as to obtain a pure product of the J1001-2 compound;
the structure of the J1001-2 compound is as follows,
Figure FDA0003499977930000021
4. the method according to claim 3, wherein the fermentation treatment is carried out for a period of 7 to 8 days.
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