CN106916835B - Biosynthetic gene cluster of compounds and application thereof - Google Patents

Biosynthetic gene cluster of compounds and application thereof Download PDF

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CN106916835B
CN106916835B CN201510992263.XA CN201510992263A CN106916835B CN 106916835 B CN106916835 B CN 106916835B CN 201510992263 A CN201510992263 A CN 201510992263A CN 106916835 B CN106916835 B CN 106916835B
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CN106916835A (en
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刘然
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Wuhan Hesheng Technology Co ltd
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Abstract

The invention discloses a biosynthetic gene cluster of a compound and application thereof. Wherein the biosynthetic gene cluster of the compound comprises: the I type linear polyketide synthase gene module comprises: 4a1 gene, 4a2 gene, 4A3 gene, 4a4 gene, 4a5 gene, 4a6 gene, 4a7 gene, 4A8 gene, 4a9 gene, 4a10 gene, and 4a11 gene; a glycosyl synthesis-associated gene module comprising: 4G1 gene, 4G2 gene, 4G3 gene, 4G4 gene, 4G5 gene, 4G6 gene, 4G7 gene, 4G8 gene, 4M gene and 4G9 gene; and a modifier gene module comprising: 4O gene, 4I gene, 4E gene, 4P1 gene, 4P2 gene and 4P3 gene.

Description

Biosynthetic gene cluster of compounds and application thereof
Technical Field
The invention relates to the field of bioengineering, in particular to a biosynthetic gene cluster of a J1001-4 compound and application thereof, and more particularly relates to a biosynthetic gene cluster of the J1001-4 compound, a microorganism and a method for preparing the J1001-4 compound.
Background
Polyketides are one of the most abundant groups of natural products, and are widely applied to the fields of medical use, veterinary use, agriculture and the like due to the great medicinal value of the polyketides. Such as the antibiotic erythromycin, the antiparasitic antibiotic abamectin, etc. Polyketides are formed under the catalysis of polyketide synthase (PKS), and gene clusters assembled in a modular form determine the structure and thus the biological activity of the polyketides, so that the gene cluster of a new compound has great significance for the subsequent research.
With the increasing severity of the drug resistance problem of pathogenic bacteria, the clinical need for new antibiotics is more urgent. In order to deal with the situation, people reform the existing antibiotics on one hand and accelerate the step of screening novel antibiotics on the other hand. The J1001-4 compound is a recently discovered antibiotic, encoding good bacteriostatic activity.
To date, no study related to the biosynthesis gene cluster of the J1001-4 compound has been reported.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art. To this end, it is an object of the present invention to propose a biosynthetic gene cluster encoding antibiotic-J1001-4 compounds with antibacterial activity.
According to one aspect of the present invention, the present invention provides a biosynthetic gene cluster of a J1001-4 compound, wherein the J1001-4 compound has the following structure,
Figure DEST_PATH_GDA0000919991810000011
according to an embodiment of the invention, the biosynthetic gene cluster comprises:
the I type linear polyketide synthase gene module comprises: 4a1 gene, 4a2 gene, 4A3 gene, 4a4 gene, 4a5 gene, 4a6 gene, 4a7 gene, 4A8 gene, 4a9 gene, 4a10 gene, and 4a11 gene;
a glycosyl synthesis-associated gene module comprising: 4G1 gene, 4G2 gene, 4G3 gene, 4G4 gene, 4G5 gene, 4G6 gene, 4G7 gene, 4G8 gene, 4M gene and 4G9 gene; and
a modifier gene module comprising: 4O gene, 4I gene, 4E4E gene, 4P1 gene, 4P2 gene and 4P3 gene.
The inventors have surprisingly found that the biosynthetic gene cluster of the J1001-4 compounds of the examples of the invention can control the biosynthesis of the J1001-4 compounds.
According to an embodiment of the invention, the 4a1 gene encodes SEQ ID NO: 1; the 4A2 gene encodes SEQ ID NO: 2; the 4A3 gene encodes SEQ ID NO: 3; the 4A4 gene encodes SEQ ID NO: 4; the 4A5 gene encodes SEQ ID NO: 5; the 4A6 gene encodes SEQ ID NO: 6; the 4A7 gene encodes SEQ ID NO: 7; the 4A8 gene encodes SEQ ID NO: 8; the 4A9 gene encodes SEQ ID NO: 9; the 4A10 gene encodes SEQ ID NO: 10; the 4A11 gene encodes SEQ ID NO: 11, or a pharmaceutically acceptable salt thereof.
According to an embodiment of the invention, the 4G1 gene encodes SEQ ID NO: 12; the 4G2 gene encodes SEQ ID NO: 13; the 4G3 gene encodes SEQ ID NO: 14; the 4G4 gene encodes SEQ ID NO: 15; the 4G5 gene encodes SEQ ID NO: 16; the 4G6 gene encodes SEQ ID NO: 17; the 4G7 gene encodes SEQ ID NO: 18; the 4G8 gene encodes SEQ ID NO: 19; the 4M gene encodes SEQ ID NO: 20; the 4G9 gene encodes SEQ ID NO: 21, or a pharmaceutically acceptable salt thereof.
According to an embodiment of the invention, the 4O gene encodes SEQ ID NO: 22; the 4I gene encodes SEQ ID NO: 23; the 4E gene encodes SEQ ID NO: 24; the 4P1 gene encodes SEQ ID NO: 25; the 4P2 gene encodes SEQ ID NO: 26; the 4P3 gene encodes SEQ ID NO: 27, or a pharmaceutically acceptable salt thereof.
According to an embodiment of the present invention, the biosynthetic gene cluster further comprises: a regulatory gene module comprising: 4R1 gene, 4R2 gene, 4R4 gene, 4T1 gene, 4T2 gene, 4T3 gene, 4T4 gene and 4T5 gene.
According to an embodiment of the invention, the 4R1 gene encodes SEQ ID NO: 28; the 4R2 gene encodes SEQ ID NO: 29; the 4R4 gene encodes SEQ ID NO: 30; the 4T1 gene encodes SEQ ID NO: 31; the 4T2 gene encodes SEQ ID NO: 32; the 4T3 gene encodes SEQ ID NO: 33; the 4T4 gene encodes SEQ ID NO: 34; the 4T5 gene encodes SEQ ID NO: 35.
According to the embodiment of the present invention, along the 5 '-3' end of the gene, along the 3 '-5' end of the gene, the 4T3 gene, the 4T4 gene, the 4T5 gene, the 4R1 gene, the 4R2 gene, the 4A1 gene, the 4A2 gene, the 4A3 gene, the 4A4 gene, the 4A5 gene, the 4A6 gene, the 4P3 gene, the 4G9 gene, the 4M gene, the 4G4 gene, the 4G3 gene, the 4G2 gene, the 4A2 gene, the 4E gene, the 4A2 gene, the 4O gene, the 4I gene, the 4P2 gene, the 4A2 gene, the 4P2 gene, the 4R2 gene, the 4G2 gene, the 4A2 gene, the 4R2 gene, and the 4G2 gene, The 4G8 gene, the 4T1 gene and the 4T2 gene are connected in series in sequence.
According to an embodiment of the invention, the biosynthetic gene cluster encodes SEQ ID NO: 36.
According to another aspect of the present invention, there is provided a microorganism. According to an embodiment of the invention, the microorganism encodes the aforementioned biosynthetic gene cluster. The inventors have surprisingly found that the J1001-4 compound can be synthesized efficiently, simply and at low cost by performing a fermentation treatment using the microorganism.
According to an embodiment of the invention, the microorganism is streptomyces.
According to yet another aspect of the present invention, there is provided a process for preparing a compound of J1001-4. According to an embodiment of the invention, the method comprises: carrying out fermentation treatment by using the microorganisms so as to obtain fermented microorganisms; carrying out wall breaking treatment on the fermented microorganisms to obtain a solution containing the J1001-4 compound; and purifying the J1001-4 compound from the solution containing the J1001-4 compound so as to obtain a pure product of the J1001-4 compound.
According to the method for producing the J1001-4 compound of the embodiment of the present invention, the J1001-4 compound can be efficiently synthesized by performing the fermentation treatment using the aforementioned microorganism. Then, the pure J1001-4 compound can be obtained efficiently, simply and at low cost through microbial wall breaking treatment and separation and purification treatment.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
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The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 shows a schematic diagram of the sequence of biosynthetic gene cluster of compound J1001-4 according to one embodiment of the present invention;
FIG. 2 shows a schematic flow diagram of a process for preparing a J1001-4 compound according to one embodiment of the present invention;
FIG. 3 shows a schematic flow diagram of the biosynthetic pathway of the J1001-4 compound according to one embodiment of the present invention;
FIG. 4 shows a schematic map of a J1001-4 compound according to one embodiment of the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements throughout, or elements encoding the same or similar functions. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, which are for convenience of description of the present invention only and do not require that the present invention must be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
It should be noted that the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. Further, in the description of the present invention, "a plurality" means two or more unless otherwise specified.
According to one aspect of the present invention, there is provided a biosynthetic gene cluster of the J1001-4 compound. According to an embodiment of the present invention, the biosynthetic gene cluster includes:
the I type linear polyketide synthase gene module comprises: 4a1 gene, 4a2 gene, 4A3 gene, 4a4 gene, 4a5 gene, 4a6 gene, 4a7 gene, 4A8 gene, 4a9 gene, 4a10 gene, and 4a11 gene;
a gene module associated with glycosyl synthesis, the gene module comprising: 4G1 gene, 4G2 gene, 4G3 gene, 4G4 gene, 4G5 gene, 4G6 gene, 4G7 gene, 4G8 gene, 4M gene and 4G9 gene; and
a modifier gene module comprising: 4O gene, 4I gene, 4E gene, 4P1 gene, 4P2 gene and 4P3 gene.
The inventors have surprisingly found that the biosynthetic gene cluster of the J1001-4 compounds of the examples of the invention can control the biosynthesis of the J1001-4 compounds.
According to an embodiment of the present invention, the biosynthetic gene cluster (also referred to as simply "gene cluster" in the present invention) is a biosynthetic gene cluster of streptomyces, encoding the amino acid sequence of SEQ ID NO: 36, specifically as follows:
ATGGCCGAGAAAGGGCTCGGAAGAGCCATTGCGCGATGCCGGGCGCCGCTGGCGGGATGCGCCGCCGCGCTCCTCGCGCTGAGCGGTTGTCTCGGTACCTCCGGCGAGTCGGACCGGCGGGGCGGACTGTGGGTGAACCCGGACAGCCCGGCCGCCCGGCAGGCCCGTGAGCAGCGGCGCGCCGGCCGGTACGCGGAGGCGGAGCTCATGCGGCGGATCGCCGACGAGCCGATCGCCGAATGGCTGGGCCCGCATCCGCGCGAGCGGGTCCGGTTCGTCACCGAATCCGCGGCGCGGCAGAACAGGGTGCCGGTGCTGGTCGCCTATCACATTCCGTACCGGGACTGCGGAAGATACTCCCGGGGCGGGGCGAGGAACGACGCCGAATATCTGGACTGGATCCGTGAGATCGTCTCCGGAATCGGCGAACGAAAAGCGATTGTCGTATTGGAGCCCGACGCGGTGGCCCAGGTGGTCGACGGCTGCGTTCCCAAGCCGCTGCGGGCGCCACGGCTGGCCCTGCTGCGCGACGCGGTCGCCATGCTCTCCGCGATGCCCGCCACCCGGATCTACCTGGACGCCGGCAACCCGGGGTGGATCAGGGACACCGAGCGGCTGGTGGAGCCGCTCATGAAGGCCGGCATCGACCAGGCGGACGGCTTCGCCCTGAACGTCTCGAACTTCCAGCCGACCTCGGTGACCCGCGCCTACGGCCGTCGCCTGTCCACCCAGCTCGGCAGGGTCCACTTCATCATCGACACCAGCCGCAACGGCAACGGCCCGCTCAGCCCCGCCAGGGGCGAGGGCGACGACGGCAAGAGCGGTGAGACCGGCGCCGTACGCTCCGCCAGCGACGACCAGGAGAACTGGTGCAACCCCCCGGGGCGGGCCCTGGGGGACCCCCCGAGCACCCGCACCGGCGACCCCGCCATCGACGCGTATCTGTGGATCAAGCGCCCCGGCGAGTCCGACGGGACCTGCAACGGCGGCCCCCCGGCCGGCCGTTGGTGGACGGAGTACGCGCTCGACCTGGCCCGCAACGCCATGGTGCGCGACAGATGAGGACGGGGCGGGCGCGCTCCGTCAGTGGGCCTCGGCGCGGGCCGTCGGCACCGCCGGGGCGGTGCGCCGCCCGCGCCGCGCCAGGAGCAGCCCGCCCGACCAGATAGCCATCGGCGCCAGGCACAGCGCCAGCGCCAGCAGCGCCCAGGTGCGCATCGCCGCGTGGGTGTGGCCCAGGAACAGCGAGGCGACCAGCGAGCCGCCGAAGACCACGGCCCACACCAGATGGATACGGAAGGTGGACAGCCGGTCGGCGCTGGAGGAGTCGCCCTTGGGCGTGACCACGAACCGGCTCTTGCGCCGCAGCAGCGCGTCGCACAGCGACTTGGCGTAGATCGGGGCGGACAGCGCCGAGAGCAGCATCCCCGCCACCCCGCCGGAGCCCTCCGGTTCGTGCGGGGAGACGTTGTGCCGCCGGTTCCAGATGTACAGGCCGATCTGCAGCGCCGCGGCGTCGCTGTAGAGCATCATCCACATCTCCGCCGGGATCTGGATCCCGGACGCCCCGAGCACCAGGAACAGCGCACAGGACAGCGCCGACAGCATCCAGTTGAGCGCGGTCATCGGATAGTAGGCGATCATCAGCGAGTAGTTGAGCAGCTTCCCCGGCGAGAGCGTGAACGGCGCCTTCCAGAACTGCTTGAGCACCGTCTCGTAGGTGCCGCGGCTCCAGCGCAGCTGCTGGGTGAAGAAGTCCGTCCAGGTGCTCGGGCCCTCCCCGACGGCCAGTACGTCCGGGGTGTAGACCGAGCGCCACTTCTTCCGGGTGGCGGGGTTGCGGCGGCGGTGCAGCTCGAAGCCGGTGGCCATGTCCTCGGTGATCGAGTCGTAAAGACCGCCGATGCTCCGCAGCGCGGCGATGCGCACCGCGTTGTTGGTCCCGACGAACATCGGGCCGCCGTACGCGTTGCCCGCCCGCTGGATCAGCGCGTGGAAGAGGAACTGCTGGCTCTCGGCCGCCTTGGTGACCGCCGTGTCGTAATTTCCGTACACCTGCGGGCCGACCACGAAGGCGACGTCCGGATCGCGGAAGTAACCCATCATGCGCTCGAGGAAGTTGGGCAGCGGCACATGATCGGTGTCCACACAGGCCATGAATTCATAGTCGTCGCCATGGGCGTCAAGCCAGGCGTTGTAATTCCCGTGCTTGGTCCTGGCGCGGAAGGAGCCCTTGGGCTGATTCCACTTCTCGACGCCCTTGCGGGAGAAATGCCGTACGCCCAGCCGGGCGCACAGCTCCTTGGCCTCCTCGTCATTTCCCTCGTCGAGCAGCCATACGTCCAGTCCCCCGGCGTGTCTGACCTGCTTGGCCCCCTCCAGCGTCGCCCGGACCATCTCGATCGGCTCCTTGCCGGGGACGAAGCTGGTCAGAAAGGCCACCCGGGTCCCGGGCTCGGCGGTCACCGGTACCGGGTCGCGGGCCACCAGTGTGGCGTGCGCGTTGGAGACCACGGTGAACAGGCGGAACGCCTCGATCAGCGCGATCGAGAACAGCATCGTCCGGTCCGCGGCCGTCTCCCAGCCCGCCACACCGTCGCGGTGCACCCAGTGCTGGGGCTGCAGCAGCCACAGCAGCAGCAGCGCCGAGCACACCGGCGCCGCGCACAGCAGCAGCGCCGCGCGGACCCGGTGCGGCTCCTGCGCCAGCAGGCTCCGGCAGCGCACCCGGTACGGGCGGCCGGTCGGGGGCTGCGTCAGCGGGCCCGCCAGCTGGCTGTACCGCTTGTACTCATAACGCCCGGCCGGGCCCCCGCGGCGGGAGGCCATCTCGTGGTGCGCGGGCCGCGCGGGCGGGGGAGCGACACTGGGCGCGGCACCCTTCTCGGCCGTGTCTCTGACGGGGGTAGGTGCGGACATC TGGGCACTCCCGCGGGTCGTCGGGGACGCAATATCGCGCGGCGATGACCCGGACGGCCTACATTCGTCATGTCATCGCGCGAGATGAGAAGGTAACAATCCGCTGGGGAATGGCTGTGCAAATCGGATTTTCGGCGCGCCCTGCCGCGCTTCGCCGACGCACCGGGCGGGCCGCCACTCCGACGGCCTATCGCCGCGCGTCTTACGTGATTTACATCCCGCCATCTGTCATGGTCGGATGCGATTCAGCGTGCATGTTTCGACCGCGCGTGAGGGCTGTGGGTGCGGGATGCGCGAGTAAACCCGCGCCATAAGGCGTGCGACATACGGAAGATGGGGCCCGGTCGCCCGATGAGAGTGCTGCACATCATCTCGCGCCTCGGCGCGGGTGGCGCCGAACAGCAACTACGGCTGCTGCTGCGCCACCTGCCCGCCCGCTGTGACGTCCTCACCCTGGCCGCGCCCGGCACCGCCGGCCACGGCATCCGGGCGGACGGCGTGCCCGTCACCCATCTGCCGATCGGCGTCCGCGACCCGATGGCACCGGCCCGGCTGGCGCGGTTCATCCGCGGCGGCGGCTACGACCTGGTGCACACCCATCTCTACCGGGCCTGCGTGCACGGCCGGATCGCCGCCCGGCTGGCGGGCGTCGGCGCCGTGATCGCCACCGAGCACAGCCTGGGCGAGCGCCACATCGACGGCCGCCCGCTCACCGTCGGCACCCGCGCCCGCTACCTCGCCACCGAGCGCCTGGGCTCGGCGACCGTCGCCGTCTCGGCCGCCGTCGCCGACCGGCTGCGCGGCTGGGGCGTCGCCAACCACCGCATCCACCTGATGCCCAACGGCCTGGACGCCGGCCACTTCCGCTTCACCCCCGCCGCCCGGGCCGCCGCCCGCGCCCGCCTCGGCATCCCCGCACACGCCTTCGTCGTCGGCGGGATCGGCCGGCTGGTGGCGGCCAAGCGCTTCGATGTGGCGCTGCGGGCCGTGGCCGAGGTGCCCCAGGCCCGGCTGCTGCTGGTCGGGGACGGGCCCGAGCGCACCGCGCTGCACGACCTGGCCGCCTGGCTCGGGGTGGCCGACCGCATCCGGCTGCTCGGCGAGCGCGACGGGGCGGTGGCCGGGACCGGGGACCGGCCCGCGGACATCCCCGGGCTGCTGGCCGCCATGGACGTGTTCGTGGCACCGTCCGCCGAGGAGGCGTTCGGGCTCGCCGCCGTGGAGGCCCTGGCGGCCGGGCTCCCCGTGCTCCACGGCGTCTGCCCGGCCCTCGACGAACTGCCGCCCGGCGCCGCCCCCGGCGCCCGCCGCGTCGGCCCCGGTGCCGACGCCCTGGCCGACGCCCTGCGCCAGCGGGCCGCCGCCGGCCCCGTACGGTACCCGGTGCCCGCCGCCGTCGAGCGCTACGACATCATCCGCACCGCCCGCCGTCTGACCCTGCTGTACGAACGCTCCGTCAGCTGCGGGCCGGACGGCTGTGCCCGCGGTCCCGCCGTCACCCTGCGCTGGCCGCGGAGCGGTGCTCCCGCGGGCTGACGCCGCGCACCCGCTTGAAGGCGGCGCTCAGCGCGAACGAGCTGCCGTAGCCGACCCGGCGGGCCACCGCCCCGAGGGTGGCGTCCGGCTCCCGCAGCAGATCGGCGGCCAGATCCAGCCGCCAGCCGGTCAGATACGCCATCGGCGGCTCGCCGACCAGCTCCGTGAAGCGGCGGGCCAGCGCCGCGCGGGAGCTCCCGGTCTCCGCGGCCAGCAGGGCCACCGTCCACGGATGGCCCGGGTTGTTCTGCAGCAGCCGCAGGGCGCGGCCGACCACGGGGTCGCCCAGCGCCCGGTACCAGGCCGGGGCCCGCGCCTCCGGGCGGGAGAACCACGTCCGCAGCACGGAGATCAGCACCAGATCCAGCAGCCGGTCAAGGACCGCCGTCTGGCCCGCCTCCTCCTTGACGATCTCCTCGCCGAGCAACGGCAGCAACGGCGAGTCGCACGCCTCACCCGGCACCACCAGCAGCGGGGGCAGCGCGTCCAGCAGCCGGCGGCTGATCTCGCCGTCCATCCGGTACGTGCCGACCAGCATCGTCGCCGAGCCGTGCTCGCTGTTGCCCCACGTCCGCACGCCCAGCGCCATCCGCTCCGCCAGGTCCTCGCCCGCGGCGGTCCGGCACACCTCGCCCGGCAGGATCCAGGCCTGGGGCTCGGTGGCCGGATCGCCGGCCACCGTGTACGGGTCGGGGCCGCGCGCGACCGCGACATCCCCCGGGCCCAGCCGCACCCCCTTCTCAGGCCCGCCCCGGTCCGGGATCACCCACGCCTCACCGCGCGCCACGGTCATCACACACAGCGGGGCCCGGTCCTCGATCCGCACCGACCAGGGCGGTTCCATGACCATCCGGAGCAGAAACGCCCCCCGGGCCCTCGGCCCGTCCAGGAGACCGGCGAGTGGATCCATCGCGCCCGCTACCTCCGGTCATCGTCACCTGCACGCCCCGAGGCTGGCACCCCGTCCCCCCGACGTCAATCGCCCCTGGGCCCTCAGGCTGTTAGGAACTCTTTAGAGGGCCTTGTGAGACTTGCGCGGACCGGTTTTCGTACCTGTCCAAGCTTGAATCGAGGCATTGTGTCTCCTTCGGAAGCCACGTCCCGTTCCGCCACCACCGCCGCCACGTGTCCCGACTTCATGGATCCGTCCTTCTGGTTACGGCCGCATGAGGAGCGGGCGGCGGTGTTCGAGAGGCTGCGCGGGCTGCCGCACCCGGAATTCGTTCCGCCGCGGCTGCCCTGGGGGCCGTTGGCAAACGGCTATTACGCGATGGTGAAACACGCCGACATCGCGGAAGTGAGCCGCCGTCCGCAGGACTTTTCCTCGGAGGGCGCCACCTCGATCCTGCCGCCGGAGATGGACGAGTTCTACGGCTCCATGATCAACATGGACAATCCGGAGCACTCCCGATTGCGGCGCATCGTGGCGCGGTCGTTCGGCCGCGGTATGGCTCCCAGGTTCGACGCCATGTCACGGCAGGTGGCCCGGCGCATCGTGGACGAGCTCATCGAGCGCGGACCCGGCGACTTCATCCGCCCGGCGGCGGAGATGCCGATCGCCGTCCTCAGCACCATGATGGGAATTCCCGGCGAGGACTACGAATTCCTCTTCGAGCGGACCAACACCATCATGGGAGGGGTCGACCCGGAACACACGGCCAGCCCCGAGGGCGCGGCGGCCGCCGTGCTGGGCGCCCTGCGGGACCTCGGGAACTACATCGGACGGCTCCGCGAGGACCGGCTCGCCCACCCCACCGGCGATGTGATCACCAAGCTGGTCCAGGTGCAGGAGGACGGCGAACAACTGACCCACCAGGAACTGGTCTCGTTCTTCATCCTCCTCGTCAACGCCGGCATGGAGACCACCCGCAACGCCATCGCCCAGGCCCTGGTACTGCTCACCACCCACCCCGACCAGCGGGAACTCCTGCTCTCGGACTTCGAGACCCACGCGAAGAACGCCGTCGAGGAGATCCTTCGCGTGGGCACGCCCATCAACTGGATGCGCCGCACC GCGACCGGCGACTGCGAGATGAACGGCCACCGGTTCCGCAAGGGCGACGAGATCTTCCTCTTCTACTGGTCCGCGAACCACGACGAGAAGGTCTTCGAGGATCCGTACCGGTTCGACATCACCCGCGACCCCAACCCTCATCTGGCGTTCGGCGCGGTGGGGCCGCACTTCTGTCTGGGCGCCCATCTCGCCCGTATCGAGATCGTCGCCATGTGCCGCGAACTCTTCACCTCACTGCCGGACATCCGCGTGGACGGCGAACCCGTCCGTGTGGCATCGAGCTTCATCGAGGGCTTCAAAGAACTGCGCTGCACATTCTGACCACACTCTGACCACGTTCTGACGCCGACAGCCAAGAAGAAAACCGCCAGGAGGGGAGGCGCCAGCGATGGCGAAGGACGAGGCGAAGCTTCTCGACCACCTCAAGTGGGTGACGGCAGAACTGCGGGACACCCGGCAGCGTCTGCGCGAAGCGGAGTCCGCGGAACCCGAGCCGATCGCGATCGTCGGTATGGCGTGCCGTTTTCCGGGCGGGGTGGAGAACCCGGAGGACTTGTGGGAACTCGTGGCGGCCGGAGGCGAGGGGCTGACCGCATTTCCCCAGGACCGCGGCTGGGATCTGGAGAACCTTTTCGACCCCGACCCGGACAGCCCGGGAACGAGTTATGTGCGCGAGGGCACCTTTCTGCGCGGGGCCGGGGAATTCGACGCGGAATTCTTCGGTATTTCACCGCGTGAGGCGTTGGCGATGGACCCCCAGCAGCGGCAGCTGCTGGAGGTCGCCTGGGAGACCTTCGAACACGCCGGCATCGACCCCCAGGGCCTCGTGGGCAGCAGCACGGGCGTCTACGCCGGGGTGACCTCGCAGGAGTACATGGTGCTCACCGCCATGGCCGGCAGCGAGGTGGAGGGCTACGCCGCCACCGGGAACCTCGCATGTGTGCTCTCCGGCCGGGTGTCGTATGTGCTGGGTCTTGAGGGTCCGGCCGTCACGGTGGACACGGGCTGCTCGTCGTCCCTGGTGGCGCTGCACTCCGCGGTGCAGGCGCTGCGCGGGGGCGAGTGCTCGTTGGCGTTGGCGGGTGGGGTGACGGTGATGGCCACTCCGGGTGCGTTTGTGGAGTTCTCGCGGCAGCGGGGGTTGGCGCTGGACGGGCGGTGCAAGGCGTTCGCGGGGGCGGCGGATGGTACGGGGTGGGGTGAGGGGGTGGGGTTGCTGCTGCTTGAGCGGTTGTCGGATGCGCGGCGCAATGGTCACAGGGTGTGGGCGGTGGTGCGTGGGTCGGCGATCAATCAGGACGGTGCGTCGAATGGGCTGACGGCGCCGAACGGTCCGTCGCAGCAGCGGGTGATCCGGCAGGCGCTGGCCAACTCCCGGCTCTCGCCGGCGGATGTCGATGTGGTCGAGGCACACGGGACGGGCACCACCCTCGGCGACCCGATCGAGGCACAGGCGCTGTTGGCGACCTATGGCCAGGCGCGGCCCGAGGAGCGGCCGCTGCGGCTGGGGTCGATCAAGTCCAATATCGGCCATACCCAGGCCGCCGCCGGTGTCGCCGGTGTCATCAAGATGGTGATGGCGATGCGGCATGGGGTGTTGCCCAAGACGTTGCATGTGGACGCGCCGACGCCGCATGTGGACTGGGGTGGCGGGGCGGTCGAGCTGCTGACGGAGGCCACCGCCTGGCCGGAGGAGGCGGGGCGTCCGCGGCGGGCGGGTGTCTCGGCGTTCGGCATCTCCGGCACCAACGCGCATGTGATTCTGGAGGAGGCGCCCGAGCCCCCCACGGAGGGGGAAACCACCGAGCCGACCGCTCCGGGCGGTGTGGTGCCGTGGGTGGTCTCCGGGCGGTCGGCCGCGGGGCTGCGGGCCCAGGCCGGACGGCTGGCCGAGTACGCCGAGCGGACCACCGCCGATGCGGAGGAGGTGGGGTGGTCGCTGGCCGCCGGCCGGGCGATGCTCGAACACCGGGCCGTGGTCATCGGCGGCGACCGGGAGGAGCTGCTGGCCGGGTTGGGGGCGTTGGCGGAGGGTGTGCCGTCGGGTGGGGTGGTGTCGGCGGAGCCGGTGGCGGGGGGTGCCGGGCCGGTGCTGGTCTTCCCGGGGCAGGGGGGTCAGTGGCTGGGGATGGGGGTGGAGCTGCTGGATTCTTCGCCGGTGTTCGCGGGGCGGATCGCGGAGTGTGAGGCGGCTTTGGCGCCGTATGTCGACTGGTCGTTGACGGGTGTGTTGCGGGGCGAGGACGGGGTGGATGTGGCGCGGGTGGATGTGGTGCAGCCCGCGTTGTGGGCGGTGATGGTGTCGTTGGCGGAGGTGTGGCGTTTCCATGGGGTGGTGCCTGCGGCGGTGGTGGGGCATTCACAGGGCGAGATCGCTGCCGCGGTGGTGGCGGGGGCGTTGTCGCTGGAGGATGGTGCCCGGGTGGTGGCGTTGCGTTCGCAGGCGTTGCGGGTGTTGTCGGGGCGGGGTGCGATGGCCTCGCTTGCGGTGGGGGCTGAGGAGGCGGAGAGGGCCATCGCGGGGCGTGAGGGTGTTGTGGTGGCGGCGGTGAACGGGCCTGGTTCCACGGTGGTCTCCGGGCCGCCGCAGGCGGTGTCCGAGGTGGTCGCCGTGGTGGAGGCGTCCGGTGGTCGGGCGCGGCTGATCGATGTGGACTACGCCTCGCACGGTCCGCAGGTGGACGAGATCGCCGAGGACCTGGTGGCCCTGCTGGGGCAGATCACACCGGTGGACACCACGGTGGGGTTCTATTCGACGGTGACCGGCGGCCGGGTGGAGACCACGACGCTGGACGCCAGGTACTGGGTGGAGAACCTGCGCCGTCAGGTCCGTTTCGCCACCGCGATCGAGGCGGTGCTGGCCGAGGGCTATCGGGTGCTGGTGGAAGCGGGCCCGCATCCGGTGTTGAGCGTGGGCATCCAGGAGACCGCTCAGGAGCGTGAGGTCGCGGTGGCCACCGTGCCGACCCTGCAGCGGGATCAGGGCGGTCCGGCGCAGCTGGCCCGGGCCCTGGCGCACGCCTTCGCCGCCGGGCTGCCGGTGGACTGGAGGGCCTGGTACGGGGTGAGCACGAACGCCGACGCCGCCACCACCCGGGCCCCGGCCGTCCTCGACCTGCCCACCTACCCCTTCCAGCGCCGGCACTACTGGCTGAAGCCGGGCACATGGCCGGGCTCCCAGGAGAAGGGCTCCGAGGAGGACGAGCGCTTCTGGAGCGCCATCGAGGCGGGGGACCTCGCCGGACTCGGGGCGTCGCTGAGCCTGGGGGAGGACACCGCGCGGCAGGTGCTGGCTCCGGCGCTGCCCGTGTTGGCGCAGTGGCGCCGGCGTACGCGCGACCAGGCCCGGATCGACGCCTGGCGCTACCTGACCGCCTGGTCCGCCGTGACCGGCCTGGACGAGACTCCCCGGCTGAGCGGTACATGGCTGCTGGTGGTGCCCGAGAAGCTGGTCGGGGACCCGGTGGCCGAGCTGGTGGCCGGGGCGCTCGCGGACCATGGGGCGCGGTGCGCGGTCTTCGCGGTGGCGCCG GAAGACCAGGAGCGCGGCCTCCTCGCGACGCGTCTGCGGCAGGTGGAGGGCTGGCCCGAAGTCGTCGGCTTGGTCAGCGTATTGAGCCTGGACGAGGCCGGACACCCGGAGCATCCGCACCTGTCGGCGGGGCTGGCCGGGACACTCGCCCTGGTGCAGGCGCTGGACGAGCTGGAGTTCGCCGGGCGCCTGTGGTGTGTCACGCGCGGGGGCGTGGCCGTCGGCCAGGACGGCTCGGTGAGCCCGGTGCAGGCGCAGGTGTGGGGCCTGGGGCGGGTGGTGGCCCTGGAGTACCCGAAGCGGTGGGGCGGCCTGGTCGACCTGCCGGCGGCCGGGGAGGAGGGGACGCCGAGCCGGCTGGCCGCGGTGCTGGCCGCGGGCACGGAGGACCAGGTGGCGTTGCGCGCCGGGGGCGCGCTGGGACGCCGGTTGCGTGCGGCGTCGGGCGGCGCGCCGGATGAGGAGTGGCGGATCGAGGGGTCGGTCCTGGTGACCGGCGGGACGGGCGGAGTGGGTGGCCGCGTCGCACGGTGGGTGGTGGAGCGCGGGGCCCGCCATGTGATCGTGGCCAGCCGGCGCGGCCCCACGGCGCCGGGGACCGAGGAGCTGACGGCCGAACTGGAGGGGCTTGGCGCCTCGGTGTCCGTGGTGGCCTGCGATGTCGCGGACCGCGACGAGGTGGCCGGGCTGCTGGCCCGGGTGCCGGAAGGGCAGCCGCTGCGCGGGGTCTTCCACGCCGCCGGGGTCGGCGACTACACGCCGGTCGGCGACCTGGACCCGGACCGGGTGGCACAGGTGCTGGCCGCGAAGGCGGGGGGCGCGCGGTGGCTCGACGAGCTGACGCGCGACATGGACCTGTCGACGTTCGTGATGTTCTCCTCCGGCGCCGCCAGCTGGGGCAGTGGCCAGCAGGGCGCGTACGCGGCGGCCAACGCGTATCTGGACGCGCTGGCCGAACGCCGCGGCGCCGAGGGCCTGCCCGGCCTGTCGGTGGCGTGGGGCCCCTGGGGCGAGGCGGGCATGGCCGCCGATGACACGGTCGCGGCCTTCTTCCGCGAGCGGGGTCTGACCGCCATGGCGCCGGAGCTGGCGCTGCGGGTGCTGGGCGACGCCCTGGGCCGCGGCGAGACGGCGCTGACGGTGGCCGACTTCGACTGGCGGCGGTTCGCCGCGACATTCGCCGGCCAGCGCGCCAGCCAACTCCTCGCCGACATCCCCGAGGCCGTCGAACTGCTGGAGAAGGAGACGCCGTCCGAGGACAGCCCGTTGCGCAGGCAACTGGCCGCGGCTGCCCCGGAACAGCGCCACCAGATACTCTCGCAGCACATCCGCGCCCTCGCGGCCGGCGTGCTGGGCCACGCGGGCCCGGACGCGGTGTCCGCGACCAAGCCCTTCTTCGAGATGGGCTTCGACTCGCTGACCGCCGTCGAGCTGCGCAACAAGCTGAGCTCCAGCGCCGCGATGCCGCTGCCCACCACGCTGATCTTCGACTACCCCACCGCGGACGACCTCGCCCGCCACCTCCTCGGGGAGATCTCCGGCACACAGGCGGCCGCCACCGGCGCCGTCGTACCCGCCGCGGGACCGGCTGGGGAGGCTGACGAGCCGATCGCCATCGTCGGCATGGCCTGCCGCTTCCCGGGCGGGGTGAGCAGCCCCGAACAGCTCTGGGACCTCGTGGCCGAGGGCCGGGACGCGATGTCGGCGTTCCCGACCGACCGCGGCTGGCGCCTCGACACGCTGTACGACCCGGACCCGGACCGCCCCGGCACCAGCTATGTGCGCGAGGGGGGATTCATCTACGACGCGGGGGAGTTCGACGCCGACTTCTTCGGGATCTCCCCGCGTGAGGCGGTGGGGATGGACCCCCAGCAGCGGCTGCTGCTGGAGACCGCCTGGGAGACGTTCGAGCGGGCCGGTATCGACCACGGCTCGCTGCGCGGCACTGACACCGGCGTCTACGTCGGCGCGACCATCTTCGACTATCTGTCGATCATCGGCATCTCCAGCATGGACATGGAGGGGTACAAGGGCACGGGCAATCTCGGGTGTGTGGTGTCCGGGCGGGTGTCGTATGTGCTGGGGCTCGAGGGCCCGGCCGTGACGGTGGACACGGGCTGCTCGTCGTCCCTGGTGGCGCTCCACTCGGCGGTGCGCGGGCTGCGCGGCGGGGAGTGCTCGCTGGCGCTGGCGGGTGGCGTGACGGTGATGGCCACGCCGGGTGCCTTCGTGGAGTTCTCGCGGCAGCGGGGGCTGGCGCTTGACGGGCGGTGCAAGGCGTTCGCGGGGGCGGCGGACGGTACGGGGTGGGGCGAGGGCGTAGGGCTGCTGTTGTTGGAGCGGCTGTCGGACGCGCGGCGCAACGGTCATCAGGTGTGGGGCGTGATCCGCGGAACGGCCATCAACCAGGATGGCGCGTCGAATGGGTTGACGGCGCCGAACGGTCCGTCGCAGCAGCGGGTGATCCGGCAGGCGCTGGCCAACGCCCGGCTCTCGTCGGCGGATGTCGATGTGGTCGAGGCGCACGGCACGGGCACCACGCTCGGCGACCCCATCGAGGCGCAGGCCGTGCTGGCCACCTACGGTCAGGAGCGGCCGCGGGAACGGCCCTTGTGGCTCGGCTCGGTGAAGTCCAACATCGGCCACACCCAGGCCGCCGCCGGAGCTGCCGGTGTCATCAAGATGGTGATGGCGATGCGGCACGGTGTGTTGCCCAGGACGTTGCATGTGGATGAGCCGACGCCGCATGTGGACTGGGGCAGCGGGGCCGTTGAACTGCTCACGGAGGCCACCGCCTGGCCGCAGGAGGTGGAGCGTCCGCGGCGGGCGGGTGTCTCGGCGTTCGGCATCTCCGGCACCAACGCGCATGTGATCGTGGAAGAGGCGCCCCAGGAGCCGGACGCTCCGGCCGGGCCCGACCCGATGCCGGTGGGCGGTGTGGTGCCGTGGGTGGTGTCCGCGCGCTCGGCCGCGGGGCTGCGGGCCCAGGCCGAGCGGCTGGCCGAGTACGCCGAGGAGGCGCAGGCCGACCCCGCGCAGGTGGGGTGGTCGCTGGTGGCCAGCCGACCGACGCTCGAGCAGCGCGCGGTGGTGGTGGGCCAGGACCGGGAGGAGCTGCTGGCTGGGTTGGGGGCGTTGGCGGAGGGTGTGCCGTCGGGTGGTGTGGTGACTGCCGAGCCGGTGGCGGGGGGTGCCGGGCCGGTGCTGGTCTTCCCGGGTCAGGGGGGTCAATGGCGGGGGATGGGGGTGGAGCTGCTGGATTCTTCGCCGGTGTTCGCGGGGCGGATCGCGGAGTGTGAGGCGGCGTTGGCGCCGTATGTCGACTGGTCGTTGACGGGTGTGTTGCGGGGCGAGGACGGGGTGGATGTGGCGCGGGTGGATGTGGTGCAGCCCGCGTTGTGGGCGGTGATGGTGTCGTTGGCGGAGCTGTGGCGTTTCCATGGGGTGGTGCCTGCGGCGGTGGTGGGGCATTCACAGGGCGAGATCGCCGCTGCCGTGGTGGCCGGGGCGCTGTCGCTGGAGGAC GGTGCCCGGGTGGTGGCGTTGCGTTCGCAGGCGTTGCGGGTGTTGTCGGGGCGGGGGGCGATGGCCTCGCTTGCGGTGGGGGCTGAGGAGGCCGAGAAGGCCATCGGTGGGCGTGACGGGGTCGTGGTGGCCGCGGTGAACGGTCCGGGTTCCACCGTGATCTCGGGTCCGCCCGAGGCGGTGGCCGAGGTGGTCGCGGGGGTCGAGGCGTCCGGCGCGCGGGCGCGGCTGATCGATGTGGACTATGCCTCGCACGGTCCGCAGGTGGATGAGATCGCCGAGGATCTGGTCACGCTGCTCGGGGAGATCACACCGATCGGCACGGGGGTCGCGTTCTACTCGGCCATGACCGGCCGCCGGGTGGAGACCACCACGCTGGACGCGACCTACTGGGTGGAGAATCTGCGCCGTCAGGTGCGGTTCGCCACCGCGATCGAGGCCGTGTTGGCCGACGGATACCGCGTGCTCGTCGAGGCCGGTCCGCACCCGGTACTCAGCGTGGGCATCCAGGAGACCGCGCAGGCGGGCGAGGTCGCGGTGGCCACCGTGCCGACCCTTCAGCGGGATCAGGGTGGTCCGGCGCAGCTGGCCCGGGCGCTGGCCCATGCCTTCGCGGCCGGGCTGCCGGTGGACTGGAAGGCTTGGTACGGGGTGAACGCCACCGCCGACGCCGCCTCCGGTGCCACCACCCGAGCCCCGGCCGTCCTCGACCTGCCCACCTACCCCTTCCAGCGCCGGCACTTCTGGATCGATCCCGCCACCCTCAACGGCTCCGGCGACGCCCGGAGCCTCGGCCTCAGCTCGGCCGGCCACCCGCTGCTCGGCGCCGCCGTCCGGGTCGCCGACAGCGGGGAGGTCGTGCTCACGGGCCTGATCTCCACCGGCGGCCACTCCTGGCTGGCCGACCACGCCGTGGCCGGTGCGGTGTTCCTGCCCGGCACGGCGTTCGTCGACCTCGCCGTGCGCGCGGGGGACGAGGTCGGCTGTGACCGCGTCGAGGAGTTGGTCCTCGAAGCCCCACTGATCCTCCCTGAGGAGGGAGCGCTGCAACTCCAGCTCGTCGTCTCCGCGCCGGGCCAGGACGGCAGCCGCGCCTTCACCGTCCACGCACGCGACGACGGCCGCGCCGACGGCCACGACGACGCCGGCCGGACCTGGACCCGGCATGCGAGCGGCACCCTCACCACGGGCGCCCGGCCGGACGACTTCGACTTCACCCAGTGGCCGCCGGCCGACGCCGAGCCGCTGTCCCTGGACGGTCTCTACACCGGCCTGGCCGAGGCGGGCTACGGCTACGGGCCGGCGTTCCGCGGCCTGAAGGCCGCCTGGCGCCGCGGGGACGAGGTGTTCGCCGAGGCCGCTCTGCCTGAGGAACTTCGGGAGGAGGCCGGGCGTTTCGGGTTGCACCCCGCCCTGCTGGACGCCGCGCTGCAAGCCGGTGGCATCGGCCAGGGCGGTACGCCCGACGGGCAGATGCTGCTCCCGTTCACCTGGAGCGGTGTGTCGCTGTACGCCACCGGGGCCGCCGCGCTGCGTGTCCGTATGGTGCCGAACGCGACGGCCGACGGCGTGTCGTTGAGCGTCGCGGACCCCTCGGGCCGTCAGGTCGCGTCCGCCGAGGCGGTGGTCTCCCGCCCGGTCTCCCTGGAGCAGCTGCGCCGCAGCGACGATGCCGGGCTCGACTCGCTGTACCGCGTCGAGTGGATCTCCGCCGAACCCAAGCCCGGAGTGCTCGCCGAAAGCGCCTGGGAGGTGGTGGGCGACGACGGCCTCGGCGTCGCCGACGCGCTGGAACGGGCCGGACATTCCGTCCGTACCGCGCCGGACCTCGCGGAGGTGGCGCGCTCCGTCGACGACGGCACACCGCCCGCCGAGCTGGTGGTGCTGCCCTGTGCGCCCCTCGCCGGCGCGGTGACCGAAGCCGACCCCGGGCTCGCCGCCGCGGTCCACGACGAGACCGCGCGCCTTCTCGGGGTCGTCCAACACTGGCTCGCGGACGAGCGGTTCGCCGACTCCCGGCTGGTGCTGGTGACCCGTGGCGCCCAGTCGACGGGCGGCGACGAGAGGGTCGCCGACCTGGTGCACTCCGCCCTGTGGGGCCTGGTGCGCTCCGCGCAGCTCGAGAACATCGACCGGCTCCTGCTGGTCGACCTCGACGCCGCCGGCCAGGACACCTCCGCCGACCAGGGCGCGGCGGCCCTGCTGCCCAGTGCCCTCGCCGTCGCGCTCGGGGCGGGCGAGCCGCAGGTGGCGGTGCGCCACGGCGGAGTGGTGGTGCCGCGCCTGGCCCGTATCGGCCAGGACACCGACCTGACCGAGCCGGACCTGCCCGGCCGCCGCCTGGACACCGAGGGCACCGTCCTGATCACCGGCGGCACCGGCACCATCGGCGGCATGCTCGCCCGCCACCTGATCACCGCCCACGGCGTCCGCCACCTGCTGCTGACGAGCCGCCGGGGTGGCGACGCGCCCGGCGCGGCCGAGCTCCGCGACGAACTGGCCGCTCTGGGCGCCGAGGTCACAATCGCCGCCTGCGACACGGCGGACCGCGACGCGCTGGCGGAGCTGCTGGCGGGCATCCCCGCCGCGCACCCGCTGACCGGCGTCGTGCACGCCGCCGGAGTGACCGACGACGGCACCATCCCGTCCCTCACGGCCGGGCAACTCCACAAGGTGCTGCGGCCCAAGGTGGACGCGGCGGTCCATCTGCACCAGCTGACCCGTGAGGCGGACCTCGCCGCGTTCGTGCTGTTCTCCTCGACCGGCGGCGTGGCCGGTGTCGCGGGCCAGGGCAACTACGCGGCGTCGAACGCCTTCCTCGACGCGCTCGCCCAGGACCGCGGGGCCGCCGGGCTCCCCGGCCAGTCGCTCTCCTGGGGCCACTGGGAGCAGAGCAGCGGCATCACCGGCGCCCTGGCCGAGCGCGACATCGCCCGGATGGAGCGTTCCGGTATCCGGGCGATGTCCTCCGACCAGGGCCTGGCACTGTTCGACGCGGCCGTGCGGCGGCCCGAGAGCCTGCTGGTGCCCGTCCGCCTCGACGCGGCGGCGCTACGGGACCTGGCGGGCGCCAACGTCCTGCCCCGTATCCTCAGCGGCCTGGTGCGGGGGGCACCGGTTCGCCGCGCGGCCGCGGGGCAACCCGCCGGCTGGGACGCCGCGTCGATGGCGGAGCGCCTGGCGGGCCTCTCGGCGGTCGAGCAGAGCCGTACGCTGCTGGAGCTGGTGCGCCGCAACGTGGCGGCCGTGCTCGGGATCGGCGACGTACTCGCGGTCGACCCGGCGCGGCCCTTCAAGGAGATCGGTTTCGACTCGCTGACCGCGGTCGAGCTGCGCAACCGGCTCAGCCAGGCCACCGACATCAAGCTGCCCTCCACGCTCATCTTCGACATCCCCACCCCGGCGCTGCTCGCCGAGCACCTGCGGGACCAGCTCGTCTCCGAGGGGCCGAGCGGGTCGGAGGCCCTGATCCAGGAACTGGACCGGCTGGAGGACCACGTCGATCTGCTCGCCGGCGACGAGGAGCGGGAC GCCGTGACGGCCCGGCTCGAAGCGCTGCTGGCGCGGTGCCGCCAGCAGCCCGCGGCGGCGGAGGGCAACGGCGTCGCGGAACGGCTCCAGGCGGCCTCGGCCGATGAAGTGCTGCAATTCATTGACTCCCAGCTGGGAAGGGCCTGAAAGAGGACCGCCATGGCGAACCAGGAACAGCTGGTCGACTACCTCAAGCGAGTGGCGACCGATCTGCATGACACTCAGCAGCGCCTCCGCGAGGTGGAGGCCCGGGACCGCCAGCCGATCGCGATCGTCGGTATGGCCTGCCGGTTCCCCGGCGGGACGGACACCCCGGAGGCCCTGTGGGAGCTGGTGTCCACCGGGCGCGATGTGATCTCGTCCATGCCGGACGACCGGGGCTGGGACCCCGGCCTGTTCAACGACTCGGGCGAGACGGGCACCAGCTATGTGCGCGAGGGCGGATTCGTCCGGGACATCGCCGACTTCGACGCCGACTTCTTCGACATCTCGCCGCGCGAGGCCCTGGCGATGGACCCGCAGCAGCGGCTGCTGCTGGAGACCGCCTGGGAGGCCATCGAGCGCGCCCGTATCGACGCGACCTCCCTGCGCGGCAGCAAGACCGGTGTCTTCGTCGGCGGCAGCACCATCTACTACGCCGGCAGCGGCGCCGACGCGCCGCAGGACGTCGCCGGGTACATGGCCACCGGGCTGGCCGCGAGCTCGATGTCGGGCCGTATCTCGTACACCCTCGGCCTGGAGGGCCCCTCCTTCACGGTGGACACCGCCTGCTCCTCGTCCGGGGTGGCGCTGCACCTGGCGGTGCAGTCGCTGCGCAAGGGCGAGTCCTCCCTGGCGCTGGCCGGCGGGGTGTGCGTGATGGCCACGCCCGGCACGTTCCTGGAGTTCAGCAAGTTGAAGGGGCTCGCAGGCGACGGCCGGTGCAAGGCGTACTCGGCGGCGGCCGACGGCTTCGGGCCCGCCGAGGGCGTGGGTGTGCTGCTGGTGGAGCGGCTGGCGGACGCCGAACGACTCGGCCATCCGGTGCTGGCCGTGATCCGCGGCTCCGCGATCAACCAGGACGGCGCCTCCAACGGCCTGACCGCGCCCCACGGCCCCGCCCAGGAGCGCGTCATCCGCGCCGCGCTGGCCGACGCCCAGCTGTCCGCCCGGGACATCGACGTGGTGGAGGGGCACGGCACCGGCACCTCGCTGGGCGACCCCATCGAGGCGCAGGCGCTGATCGCCGCCTACGGCAGGAGGCGCGCCGAGGGCAGCCCGCTGTGGCTGGGCTCGGTCAAGTCCAACATCGGGCACACCCAGGCCGCCGCCGGCATGGCCGGGGTGATCAAGATGGTGTACGCCCTGCGCCACGGCCTGCTGCCGCGCACCCTGCACGTGGACGAGCCCACCACCCAGGTGGACTGGTCCGAGGGCACCGTGAAGGTGCTGACCGAGGCGCGGCCGTGGCCCGAGACCGGCGCCCCGCGCCGCGCCGGTGTGTCGTCGTTCGGCATGAGCGGCACCAACACGCACGTCATCCTCGAACAGGCCCCGCCCGCCGATGAGCCCGCCCCAGCCGCAGAGTTGCCGGTCGTCCCCTGGCTGGTGTCCGGGCGTGGCGAGGCGGCGCTGCGCGCCCAGGCCGCCCGGCTGCGGGACTTCCTCGCCGAGCGGCCGGAGGCGACCCCGACCCGTGTCGGTTTCACCCTCGCCCATGCGCGCGCCGCCCAGTCGCACCGCGCCGCCGTGGTCGCCACCGACCGCGACGCCCTCCTGGCCGGGCTGGCCTCGCTCGCCGAGGGCACCCCCTCCGGCCAGGTGGTCACCGGTGTGCCGAGCCCCGGCGCGACGGTGTTCGTCTTCCCCGGCCAGGGCTCGCAGTGGCTCGGCATGGCCCTCGCCCTGGCCGACGCCTCCCCGGTGTTCGCCGAACACTTCCGCCGCTGCGCGGCCGCGGTGGAGCGGCACACCGACTACACCGTGGAGTCGGTGCTGCGGGGCGAGCCGGACGCCCCCTCCCTGGAGCGGGTCGACGTGGTCCAGCCCGTGCTGTGGGTGGTCATGGTGGCCCTGGCCGAGCTGTGGCGTTCGTACGGTGTGGAGCCCGCAGCCGTGGTCGGCCACTCCCAGGGCGAGATCGCGGCGGCCTGCGTGGCGGGCGTGCTGTCGGTGGAGGACGCGGCCCGCGTCGTGGTGCTGCGCTCCCAGGCGCTGCCCGAGCTGTCCGGCCGTGGCGGCATGGCCTCCGTGCCCCAGCCCGTCCAGCAGGTCGGGAAACACCTGGAGCGCTGGGACGGCCGGCTGAGCGTGGCCGCCGTCAACGGCCCCTCCTCGACCGTGGTCTCCGGCGACGCCGACGCCCTGACCGAACTCCTGGAGGGCTACGAGGCCGACGGGGTGCGCGCCCGCCGCGTCCCGGTGGACTATGCCTCGCACTGCGCCCACGTCGACGCGCTGCGCGACCGGCTGGCCGAGGCCATCGCGGACATCGAGCCGAAGGCGGGCTCCGTCCCGCTGTACTCGACCGTCACCGGGCGGCGCATCGACGGCACCGCCATGGACGCCGCGTACTGGTTCACCAACCTCCGTCAGCAGGTGCGGTTTCAGCAGGCCACCGAGGCGCTGCTCGAGGACGGGCACAGTGCGTTCATCGAGTGCAGCCCGCACCCGGTCCTGGCCATCGGCACCCAGGAGACCATCGACGAAGCAGGTGTGGCCGCCACGGCGCTCGGCACCCTGCGCCGCGACGAGGGCGGCTGGGACCGCTTCCTCCTCGCGCTGGCCCAGGCCCATGTCCACGGTGTCGCGGTGGACTGGAGCCGGGTGTTCCCGGACCCGACCATCCCCCTCGACCTGCCCACGTACGCGTTCCAGCGCCGGCGTTACTGGCTGGACGCCGGCGGCACCGGGACCGGCGACCCCACCCGCCTCGGTCTCGCCCCCGCCGACCATCCGCTGCTGGGCGCCGTCACCGTCCTCGCCGAGGGCGACGAGGTGGTGCTGACCGGCCGGCTCGGCCTGGACACCCATCCGTGGCTGGCCGACCACGCGGTGGCCGGCGCGGTGCTGGTGCCCGGAGCCGTGTTCGTGGAGCTGGCCGTGCGGGCCGGCGACGAGGTGGGCTGCGACCGTGTCGAGGAAATGGTGCTGGCCAGCCCACTGGTGCTCGCCGACGAGGGCGGCACCGACCTCCAGCTCGTGGTGGGCGGGGCCGACGAGAACGGCCGCCGTCCCTTGAGCGCGTACGCCCGCCCGGCCGGGACCGACCAGCCGTGGGTGCGCCACGTCAGCGGCACGCTGGCGACCGGCGGGGCCGCCGAGCCGTTCGACCTCGCCCAGTGGCCGCCGGAGGGCGCCGAGCCCGTGTCCGTGGACGGCTGCTACGACCAGCTCGCCGAGGGCGGCTACCGCTACGGGCCCGCCTTCCGCGGCCTGCGCGCCGTCTACCGGCGCGAGAGCGAGGTCTTCGCCGAGGTGGTCCTGGCGGAGGACCAGCGCGGGCAGGCCGCCGCGTTCGGCATCCACCCCGCTCTTCTGGACGCCGCGCTGCACGC CAGCGGGCTGACCGCGGTGCGCGGCGACACCGCGGGCCGGATGGCGCTGCCGTTCGCCTGGAACGGCGTGTCGCTGGCCGCCTCCGGCGCCGAGTTGCTGCGCGTGAGGCTCGCACCGGTCGGCGACGACGGCATGTCCGTCCACGCCACCGACGCCTTGGGACATCCGGTGATCTCGATCGAGTCCCTGGTCACCCGGCCGTTCACGGCCGGGCAACTCCCCAGCGGCGCCGCGGAGGACACCCGCGACGCACTCTTCCGCGTGGCCTACGTCCCTGTGACCGACGCCGGGCCCGACCCGCGCGTCTCCACCGACGACTGGGCCGCGGTCGCCGCCGGCACCTCGCACAGCCGCTACGGGGCGCGCCGCCACGGCGACTTCGACGCGCTCGCCACCGCGGTGGACGGCGGTCTGCCCCTCCCGCCGGTGATCGTGCTGCCCTGCGATCCGGCGCCGCACGACCCGGACCTGGCCGAGGCCGTGCGGCGCCGGCTGGGCGAGGTGCTGCACACCGTGCAGCGGTGGCTGGCCGACGAGCGGTTCGCCGGGGTGCGGCTGGCAGTGGTCACCCGTGGCGCGGTCGCCGCCACCGACGGCGAGGACGTCACCGACCTCGTGCACGCCCCGGTGTGGGGCCTGATCCGCTCCGCGCAGTCCGAACACCCCGACCGGCTGGTCCTGCTGGACGTGGATGCGGACGGGGCCGGACACGAGCTGCCGGAGGCCGTCGCCGCCGCGGTGGCCGCGGGGGAGAAGCAGATCGTCGTCCGGGACGGGACGGTCCGGGTGTCCCGCCTGGTCCCCGCCGCGTCCGGCGGCGCCCTGACCCTCCCGGAAACCCCCCACTGGCGGCTCGACATCACCGGCCCCGCCACCCTGGACAACCTGGACCTGGTGGCGGCGGACGAACCCCAACCGCTCGCCCCCGGCCACCTCCGCGTCCAGGTCCGTGCCGCGGGCGTCAACTTCCGGGACGTACTGATCGCACTCGGCATGTACCCGGGCGAGGCGGGCCTCCGGGGCAGCGAGGGCGCGGGCGTGGTGGTCGAGGTGGCCGACGACGTGACGTCGGTGTCCGTCGGTGACCGCGTGATGGGCATGTTCCAGGGCGCTTTCGGGGCGACCGCCGTGGCCGACGCCCGCTCCGTCGTGCCGATCCCGCCGGGCTGGACCGACGAGCAGGCGGCGGCCGTGCCCATCGCGTTCGTCACCGCCTGGTACGGGCTGGTGGACCTCGCGGGCCTCACGGCGGGCGAGTCGGTGCTCATCCACGCGGCGGCCGGCGGGGTGGGCATGGCCGCCGTGCAGATCGCCCGCCACCTGGGCGCCGAGGTGTACGCCACGGCCAGCCCCGGCAAACACGCCGTACTGGAGGCCATGGGCATCGACGAGGCCCACCGCGCCTCCTCCCGCGACCTCGACTTCGAGGAAGCCTTCCGCGCCGCCACCGGCGGCCGCGGCGTCGACGTGGTCCTCAACAGCCTCACCGGCGCCGCCACCGACGCCTCGCTGCGCCTGCTGGCCAAGGACGGCCGGTTCGTGGAGCTGGGCCTGACCGACGTCCGCGACCCCGGGCAGCTCGCCGCGGTCCACCCCGGTGTCCACTACCGCGTCCTCGACCTGGGGCAACCGGGGGAGGACGGCATCGGCCGCATGCTGAGCGACATCCTCGGCCAGTTCGAACGCGACGAGCTGGCCCATCCACCGGTGCGCGCCTATGACATCCGGCGGGCGCGCGACGCCTTCCGCCTCATCAGCCGCGCCGCCCACGTCGGGAAGGTCGTACTGACCCTGCCGCGGACCCTCGACCCGGACGGCACGGTCCTGGTCACCGGCGGCACCGGCACCCTGGGCGGCCTGGTCGCCCGCCACCTGGTCACCCGCCACGGCGTACGGCGGCTGCTGCTGACCAGCCGCCGCGGCCCGCAGGCCCCCGGCGCCACCGACCTGTGCGACGAACTCGCCGAGCTGGGCGCCACCGTGACCGTCGAGGCCTGCGACGTCGGCGACCGCGCGCAGCTCGCCGAGGCACTGGCCGGCATCCCCGACGCACACCCCCTGACCGGGGTGGTGCACTGCGCCGGCGTCCTCGACGACGGCATGGTCGACGCCCTGACCCCCGGGCAACTGGCCCGGGTCCTGCGGCCGAAGGCGGATGCCGCACTGCATCTGCACGAGCTCACCAAGGACGCCGACCTGGCGATGTTCGTCCTCTTCTCCTCCGTCGTCGGGGTGTACGGCAACCCCTCGCAGGCCAACTACGCGGCCGCCAGCACCTTCCTGGACGCTCTGGCCCAGCACCGCCAGGCAGCCGGGCTGCCCGCCCAGTCCCTGGCCTGGGGCATGTGGGAGGAGACCAGCACCCTCACCGGTGAGCTGGGCGACGGCGCCCTGCAGCGGATACGGCAGGCCGGTATGGAGCCGCTGCCCACCGAGCGGGCCCTCGCGCTGTTCGACCGCTCACAGGCGGCGGGCGACGCGCTGCTGGTCCCGATCCGGCAGTCCGCGCAGGGCCCCGCCGGCGGTGCGGCCGCCGGCCGTAGCAGGGCGCGGCGGGTGGCGGACAGCGGCACCGCGAACACCTCGGGCCCGTCGCTGGCCGAACGGCTCGCCGCGCTGCCGGAGGCGGACCGCGAGGGCTGTGTACTGGAGGCGGTGTGCGTGCACATGGCGGCCGTACTCGGCCACGGCTCGGCCGACGAGATCGCCCACGACCGTACGTTCAAGGAGCTGGGCTTCGACTCGCTCACCGCCGTCGAACTGCGCAACCGGCTCTCGGCCGCGACCGGCCTGCGGCTGCCCGCCACGCTCGTCTTCGACTTCGCCAACCCGCTGGCGCTCGCCCAGCACCTGCGCGAGCAGATCGCCCCGCCCACCGACCCCTTCGAGAGCCGGGTCCGCCAGGTGCTGGCCGCCATCCCGCTGCGGCGTCTTGAGCAGTCGGGACTGCTGGAGGCGCTGCTGCGGCTGGGCGAGGGCGGGGACACGGAGACGGACGCGGAGGCGGACGCCACCGTGTCCGAGCCGGCCCCGGCCCCGTACGAAAGCGACGCCGACACCGCCGACATCGCCTCCATGGACCTCGACGAACTGGTGGACATGGCCCTGCGCAACGGCGAGTCCTGACGTGTGCCGTCCTGTCCGCCGCCCCCTGTGCCGTCCCGTCGGAGGAATGTCATGACCGTGGCCGACGACAAGATCGTCGAAGCCCTGCGCGCCTCGCTGCAGGAGGCCGAGCGGCTGCGCAAGGAGAACCAGCGGCTGCGCGCGGCCCCCCGGGAGCCGATCGCGATCGTCGGCATGGCCTGCCGCTACCCCGGCGGTGTCGAATCCCCGGACGACCTGTGGACCCTGCTGGCCGAGGAGCGCGACGCCATCTGCCCCTTCCCCCGGGACCGCGGCTGGGACCTGGACGGCATGTGCGGGCCCGACGCCCACCCGCAGGCGCGCAGCGCGGTGTCCGAGGGCGGATTCCTCGGCGGCGCCGCCGCGTTCGACCCCGCGCCCTTCGGGATCTCCCCGGGCGAGGCGT CGGTGATGGATCCCCAGCAGCGGCTGCTGCTGGAGACCTCGTGGGAGGCGTTCGAACGGGCCGGGATCGACCCGCGGTCGGTCGCCGGCCGGCGCTACGGCGTGTTCATGGGCACCACCGGCCAGGACTACACCCCGCACCTGAGGAACGTCCCCGAGGAACTGCTCGGCCACGTCGCCTCCGGCGGCTCCAGCGCCGTGCTCTCCGGCCGTATCGCCCTGACTTTTGGCCTCGAAGGGCCCACGGCCACCCTCGACACCGCGTGTTCCGGCTCCCTGGTGGCGCTCCACCTGGCCTGCCAGTCGCTGCGCGGCGGCGAGTGTTCCATGGCGCTGGCCGGCGGTGTCACCGTGATGTGCGGCCCGGACACGTTCATCGGGTCGGCCCGGGGGATAGGGCTGCCTGTCGCCGCCCGCTGCCGCTCGTTCGCCGACGGGGCCGACGGGATCGGCTTCGCCGAGGGGGCCGGGGTGCTGCTCCTGGAGCGCCTGTCCGCCGCCCGCGCCCACGGACACCCCGTGTTCGCCGTCGTCCGCGGCAGCGCGATCGGCCAGGAAGGCGCCACCAACGGCGCGAGCGCGTCGAACGGTTCCGCCCAACGGCGGCTGATCAGAAGGGCGCTGGACGCCTCCGGGCTGGCGCCGCACGAGGTCGACGCGGTCGAGGGCCAGGGCACCGGCGGACTGCTCAGCGACGCGGTCGAGGCGCAGGCCCTGGCAGCCGTCTACGGCGAGGGTCGCCCCGCCGACCGGCCGCTGCTGCTGGGCGCGGTGAAGTCCAACCTCGGCCATACGCAGGGGGCCAGCGGTATCGCCGGAGTGCTCAAGATGGTGCAGGCCATGCGCCACGGCATCGTGCCGCGCACCCTGCACACCGAGGCGCCGTCCCCGCACATCCCCTGGGACGAGGGCCGGATACGGCTGGTCACCGACGCCACACCGTGGCCCGGGACGGACCGGCCACGGCGGGCCGGGGTGTCCGCGTTCGGCTTCGGCGGTACGGACGCCCATGTGATCCTGGAGCAGGCCCCCGACGACGAACCCCCGCCCCCGGCCCCCGAGGCGGCGGGCGGCGGCGTGGTGCTCTGGCCGGTCTCCGGCTGCGACCCGGACGCGCTGCGCGCGCAGGCGGCCCGGCTGCTCGCCCATGTCGAGCGGCGGCCCGGTCTCCGGCCGGCGGACGTCGGCCTGTCGCTGGCGACGTCCCGGGCCGCGCTCGGACACCGTGGCGTGGTGGTCGGCGAGAGCCGCCAGGACCTGCTGGACGGCCTGCGGGCGCTGGCCGACGCGACACCCGCGCCCCACGTGGTCCACGGGGTGCCCGGCCGCCGCCACCGGCTCGTCGTCCTGCTCACCGGCCACGCCCTCGCCCCCGGCACCGGCAAGCAGCTCTACGACGCCTTCCCGGCTTTCGCCGGCGCGTTCGACACGGTCTGCGCGGCGCTCGACGCCCACCTGGGGTTCCCCGCCCGTGACGCGATGCTCACCGGCGCGGCCCCCGCCCCGGCCGCGGGCCTCGAAGCGGCCAGGGCCTTCGCGGTCCAGGTGGCGCTGTTCCGCCTGGCCGAGTCGTGGGGCGTGCGGCCCGGCGCCGTACACGGCCACGGCATCGGCCACATCACCGAGGAACATATCGGCGGACGGCTGGCACTGCCCGACGCCTGCGCGGCGCTGACCGGACCGCACGACCCGGGGCAGACGCCGAGGGACATCGCCGACCAGGCCCGCGCCGTCGCCAAGGACGGAACGGTCCACCTCGAACTGGGCGGCCACGAGGTGGCCGAACTCCTCGACGGCGCGGGGCAGCACGCGGTGGCCGCGCTGCGGCCCGGCGTGGCCGAGACGACCGCGGTGGCCACCGCCCTCGCCCGGGTGTACGCGCAGGGCACCGCGGTGGACTGGGACGCGGTGTTCGCCGGGACCGACGCCCGGCGCGTCGAGCTGCCCACGTACGCCTTCCAGCGCGCCCGGTACTGGAGCGCGGCGTTCGACACGCTGCGGAGCCGGCACCCCGAGGAGCTGCCCGGCCGGCGCTGAACCGAGGCGGTGCCACGAGGGGCCGCGCCCGTACGGGCGAGGCCCCTCGTGGCGTCGCCTCGGTGTGCGCCCCGGGATACCGGGTCACCGAGAGGCGGCCTCGGAGGCGCCGGCGCCCAACACCGCGGGGAGCCGGTCGAGCTGCCGGGTCCCGGGGACGGGGCGCCACGTCAGCTCCTTCTCGGGCACGGCGAGCGCCAGCCCGGGGAAGCGGCCGAAGAGCGTCTCCAGCGCCAGCTCCACCTCCTGGTTGGCGAGCATGGCGCCGAGGCAGTAGTGCGGCCCGCGCGAGTAGGCGACGTGCTGCACCCCCGTGCCGTCGGGGCGGCGGGTGATGTCCAGCCGCTCAGGGTCGGGGAAGTGCCGCGGATCGTGGTTGGCGGCGCCCTGCACCGGCAGGACGACATCGCCCCGGCTGATCAGCGTGTCCGCGACCCGGACGTCCTCCGTGGCGTACCGCGCCTTGCCGAAGACGACCGAGCTGCGCCAGCGCAGCAGCTCCTGGACGGCTCCCGGGATCAGCTCCGGATCGCTCCGGAGGAGGGCGAGCTGATCGGGGTGGGTCAGCAGGGCGAGGACGCTGTTGCCGATGACGCCGGAGGTGGTTCCGTGGCCGGAGACCACGAGGTGGAAGGCCATCGTGACCACCTCGGTCTCCGAAAGGCCCTCGCCGTCCTCGCCACGGGCCTGGATGAGCGCCGTGAGCAGGTCGTCCGCCGGTTCTACCCGGCGCTTGGCGACCAGCTCCCGCAGATAGGCCGTGGCCTGGATGAGCGTGTTCTTCAGCCGCAGGGCGTCGCTGTAGTCGTGGCTCCACCGCAGCCACATCGCCCGGTCACAGGCCGGCACGCCGATCAGCTCACAGATGATCGTGACGGGCAGGGGCTGGGCGAAGTGTTCGACCAGGTCGACCGTGCCGTCCACCGCGTGTCCCGGCAGCGCGTCCACGAGCTCCCGCGTCAGCGTCCGGATCCGCGGCCGCAGAGCGGCGACCCGGTGCGCCGAGAACACCCGGGTGACGAGCTTGCGCAGCCGGGTGTGGTCGGGCGGGTCGAGCTGTGTGATGGCGCGGGAGAGATAGGGAATCAGCTCCTCGGGCAGGCCGAGCCAGGTCAGCGACGCGGCGTGGCGGTCCGTGGTGGAGCCGGGCACCGACCGGGAGTTGCTGACGAAGCGGCTGTCGGTCAGCACGCCCGTGACGTCGTCGTAGCGGGTGACGACCCAGATGTTCCGGCCGTCGGCCAGGGTGCCCAGGGTGACCGGCGCCTCCTCCCGGATGCGGCTGAAGCCGCGGTAGGGGTCGGCGGTCAGCTCCGGGTCGTTGACCGAGACCGGGCACCGCACCGGCGTCTTCGGCGTCTCGCCTGTGGGCATCACGCCTCACCGGGCTTCCGCGCCGCCGCGATGATGTGCTCCAGGCGTACCGCCTGCCCGGTGATCGTTTTGATGTAGGCCTTCATACCCGAACCGCTCCAAGCGGGCCGCCAGGGTCTT CCTGCGGGGCGCGGGGCGCGGCACCAGGTCGGCCGTGGCGAGCACACCGCCGGGCCGCAGCACCCGGAACGCCTCCTCGAAGAACTTCTGACGGGTGTCGAAATGACACGCGGACTCCAGGGCGACCACCCGGTCGAAGGAACCTGCCTCGAAGGGGACGTCCGTCGCCGAGCCCAGGCGCAGGTCCACCCGGCCGCTCAGGTTCTTCTCCGCGGCGCGCCGCCGCCCGATCTCGACCTGGCTGCGCGTCACATTCAGGAAGCCGCGGTAGGCGCTGCGTCCGGTGAGCCTGCGGCTGTTGAGCTCGTACAGCGCGCTGACGCGGGAGGTGGGGGAGGCGGTCATCCACTGCACGAAGCGCGCGGCGGACCGGAACCCGTTCACCACCGAGTTGCCGCCGTTGTCGATGATGACCTGCTCGGTCTTCGTTGGTGTCATCTTCGGGCCTTCCGTCGTCGGCCATCCGCACCGGTCTCAGCGGAGGATCGCCGATCCGCCTAAAGCAGGCCTGTCGCCCGGGCGGGCCCCGCCCGCCCGCGCACCGGCGGAAAGGGCTGCTTAAGGCCGTTCGGCAAGGGTGACTGGCCGAGGTCCTTAAGGCCAGGGAGGACACGGTGCTCAACGAGCGCACCCGCAAGAAGATCGCACTCGAACACAGCCGGCTCCTCGGCGCCGGCGACATCGACGCGCTGATCGGCCTGTACGCGCCGGACGCCACGTTCGAGGACCCCGTCGGGACCGGTGGCCTGAGGGGACACGAGGCGCTGCGCGCACACTTCCGAAGCGCCGTCGCCGGAAACGTACGGGAGACCGTGGAGGACACGGTCGTCGGTCAGGACGGCACACACGTCCTGTCCAGGATCACCGCCGACATGGACTACCGGCCCCGCGGCCCGGTGTACGCGGACCGCGGCTGGTTGCCCCCGCCCCAGGGCGCGGAACCGGCCGGCCTGCGCTGCCACTACGCGCTGCTGCTCCGCGTCGGCGAGTCCGGACTCATCGAGGACATGCGGGCGTACTGGGGCCGGCCCGACCTCGAGGTCTCGGCGGCGGGCGCGCCCGGGACGTTCCGCGGCCCCGACGTCATCGCGCCGAAGGAGGCGGCCCTCAGGCGGTTGCCGAGCACCTACCTCGGACTGCTGGCGAAGGGGGACATCGAAGGCACCGTCGCCATGTTCGCGGAGGACATCGTCTTCGAGGACCCGGTGGGCGGCCTGCTGCTGCGCGGCAAGGACGCCCTGCGCGAGCACGTGTTCCGGGGCTCGGAGGGCAAGGTGCACGAGGTGCTGGGGCGGCCCGTGTCGTCGATGGACGGACGGTTCGTGGTGATCCGCGGAGACGCGCGGGTGTTTGTACCGGCCGAGATACGCATGCGCATGATCACCATCTGCGAACTGGACGAGGACGGCCTGGGCGTTCACATCCGCGGGTTCTGGGGCCTCACGGACACCACGTTGGGGCACTCCGAGCCCGGGACGGGCGCCGGCCCCGCGCCCCGCGCCACGGACCCGCCGGAGCCGAAAGGCCCGCTTAAGCTGTTCAGGACACCCTCGTGATGGGCCTGGCAGGCCGCAGAGAGACACCCGCGCGGACGAGCCCGTTGCGAAGGGACTTGATTCCATGACCACACCGACACGCGCCGTTGTGCTGGGCGGCGGATGGGCCGGCATGCTGGCCGCCCATGTGCTGTCCCGTCACCTGGCAACCGTGACCGTGGTGGAGCGTGACATCCTGCCCGACGGGCCGCACCACCGTAAGGGACAGCCACAGGCCCGCCATGTCCACGTGCTGTGGTCCAGCGGCGCGGGCATCGTCGAGAACCTGCTGCCGGGCACCACCGACCGGCTGCTGGCCGCCGGTGCGCGCCGGATCGGCTTCCACAGCGACCTGGTGACCCTGACCTCGGCGGGCTGGCAGTACCGCTTCCCGGCCACCGCGTACGCGATGATGTGCACCCGCCCGCTGCTCGACTGGACGGTGCGCGAGGCGATCCTCGCCGACGGCCGGGTCCGGGTGGAGCAGGGCACCGAGGCCGTCGACCTGGCCGGCGACCGCTCCCGGGTCACCGGGGTGCGGGTGCGCGACGCGGACGGTGGCGAACCCAGGCTGATCGAGGCGGACCTGGTGGTGGACGCCACCGGGCGGGCCTCGCGCCTGGGCCGCTGGCTGGCCGCCCTCGGCCTGCCGACCGTCGAGCAGGACGTGGTCGACGCCGGCATCGGGTACGCGACCCGGATGTTCAAGGCGCCCGAGGGCGCGGGCAGCGCCTTCCCCGCGATCCAGGTCGCCGCCGACCCGCTCACCCGGCAGCCCGGCCGCTTCGGCGTGGTCTACCCCCAGGAGGGCGGACGCTGGCTGGTCACCCTGACCAGCACGCGCGGCGCTCCGCTGCCCGGCGGCGAGGACGAGTTCGCGGCCTACGCCAAGGAGCTGCGGCACCCGATCGTCAGCGACCTCATCGGCGTCGCCGAACCCATCAGCCAGATCTTCCAGTCGCACTCGGGGGCCAACCGCCGGCTGTACCCCGAGCGCATGGCGCAGTGGCCGGAGGGCCTGCTGATCCTCGGCGACTCCCTGGCGGCGTTCAACCCGGTCTACGGACACGGCATGAGCTCGGCCGCCCGCGCCGCCGAGGCCCTGGACCGGGAACTGGCGCGCGAGGGGTTCGGCGAGAACCGCGGCCGGCAGATCCAGCGGGCCCTGAGCGAGGTGGTCGACGACCCGTGGATCATGGCGGGGCTGAACGACATCCAGTTCGTCAACTGCCGCAACCTGTCCCGCGACCCGCGGCTGACCGGCCCGGACACCGCGGAGCGGCTGAGGTTCTCCGACTACATCTCCAGCAAGTCGATCAGGTCGCCGAAGGTCTGCGAGGTCGCCACCGGCGTGCTGAGCCTGAACGCGCCGCAGAAGGCCCTGGGCGACGCCCGCTTCCTGTCGCTGCTGCAGTCCGACACCAGCTACCCGGAGCTCGTGGAACCGCCGTTCCGGCCCGAGGAGCTGGAGATGGTCGGGCTCAAGCCGTCCGGCACGGCCCCAAGAGGCGTACTCGGCCGGCCCACCGTCTAGCCCACCGCACACACCCGTCAGCCCGACACCACACCGAGAAGGGTGCACCCATGGAACCCACCAACGGATCCCCCGACGACGGTCAGCTGGCCGATCGGCTCGCGTCGGCGGCGCCGGAGGAACGCCAGCAGCTGCTGTGCGAGCTGGTGCTCCGCCGGGCCGCCGAGATCCTCGACGTACCGTCGCTCGACGAGGAGAGCAACTTCCTGGAGAACGGCCTGAGCTCGCTCACCGCCGTGAAGCTCTCGAAGTTCCTGATGGACGACACCGGGATCGAGGTCCCCCTCGTGTCCATCGTCGAACACCCCACCTCCACCCTGCTCGGCAAGTACCTCGCCGAGGCCTACGCGGCCGACGCCGCCTAGGCGCCGGCGCCGCGACCCGCCCGGCCGATCACCATCTCGTCCCAACTCCCAGGAGCCAGGGGAACCCCGTCATGAGGCTGTCCGTTCCGGTTGACCACGA AGTGACGTTGAACGTGCGGCACCGGCCGGGGCGCGGCGGCCGGCCGTTCCTGCTGCTCCACGGGCTGGCGTCCAACGCCAGGATGTGGGACGACGTGGCCCGCCGGCTCGCGGACGCCGGCCACCCCGTCTACGCGATCGACACGCGCGGACACGGCGACTCCGACGTACCGGAGCAGGGGTACGACAACGAGGCCGCCGTGGCGGATCTGGCGGCCGTCTGCCGCGAGCTGCGGCTGACGGGCGCACTGCTCGCCGGCCACTCCTGGGGCGGGATGGTCGCCGTGCGGTTCGCCTCCGTCCACCCCGACCTGGCCGCCGGCCTCGCGCTGGTGGACGGAGGGTGGATCAACCACACCGACACCCCGCTGCGGATCCCCACCTGGCAGAAGGACGTGGAGAAGATCGCGAACGGGGTCCCCGGCACCACGGGAGTCACCGTTCACACCGCGCGGGAGCGCATACGAACGCTCCACCCGCACTGGTCGAAGGACGCCGTCGAGGCGATGCTCGCCGACACGGTGGAAGGGCCGGACGGGCTGCTCGTGCAGCGGCTGCCGCTGAAACACCGCCTGTCGATCCTCAACAGCATGTGGAACGACTCGCCCGCCCGCTGGTACCCCGGCATCGCGGTGCCCGTCCTCATGCTCATAGCCCTGCCCTCGTACGCCTCCGCGCTCGGGGACTGGGTGCGCAAGTGGGTGACCGAGGCCGAGTCGGCCATCGCACACGTGGACTCCCGCTGGTACGTCGGCGCCGACCACAACCTCCACGCGGACCAGCCCGAGCGGGTGGCCGCCGACCTGCTCGAACTGGCCCGCAACGCGGACCGGTCGGCCCTGGAGCGGTCCTGACATGGCAGACGACGACAGGAAGCTCCTCGACTACCTCAAGCGCGTGACGGCCGATCTGCGCGAGGCGCAGCGCCGGCTCAAGGACGTCGAGTACGCCCACCACGAGCCCGTCGCGATCATCGGCATCGGCTGTCGGTTCCCCGGTGACGTGAACTCCCCCCAGGACCTGTGGGACCTGGTCGCCGCCGGCCGCGAGGGCACCGGCGGCCTGCCCACCGGCCGCGGCTGGGACCTCGACGGGCTCTACGACCCCGACCCCGGGCATCCGGGCACCAGCTATGTGCGCCAAGGCGGCTTCGTCCACGACGCCGACCGCTTCGACGCCGCGTTCTTCGGGATCAGCCCCAACGAGGCCGTCACCATGGCCCCGCAGCAGCGGCTGGCGCTCGAGGTGGCCTGGGAGGCCATCGAACACGCCCGCATCGACCCGCACACCCTGCGCTCCAGCGCCACCGGCACCTACCTCGGCTGCGACGGCCTCGACTACTTCCTCAACTCCTACCACGTACCGGAGAACGCGGCCGGCTATCTGACCACCGGCAACTCCCCGAGCGTGGTCGCCGGGCGCGTCGCGTACGCCCTCGGCCTGGAGGGCGCCGCGGTCACCCTGGACACCGCCTGCTCCTCGGCGCTGGTGTCGATGCACCTGGCCTGCCAGGCGCTGCGGGAGGGGGAGGTGACGCTGGCGCTGGCCGGCGGCGTCTACATCATGTCCTCGCCCGCGCCGCTGGTCGGGTTCAGCGAACTGCGCGCCCTGGCCCCCGACGGACGCGCCAAGCCCTTCTCGGCGGACGCCGACGGCATGAACCTCGCCGAGGGGGCGGGGATCGTCCTGCTGGAGCGGCTGTCGGACGCCCGCCGCAACGGGCACCGGGTCCTGGCGGTCATCCGCGGCTCCGCCGTCAACGAGGACGGGGCGAGCAACGGGCTGACGGCGCCCAACGGCCCGGCGCAGCAGCGCGTCATCCAGCAGGCGCTGGCCAACGCCCGGCTCTCCGCGGCCGACGTGGACGCGGTGGAGGCCCACGGGACCGGCACCAGCCTCGGCGATCCCATCGAGGCCCAGGCGCTGCTGGCCACCTACGGCCAGGACCGGCCGGAGGGCCGGCCGCTGTGGCTGGGGTCGATCAAGTCCAACATCGGGCACACACAGATCACCGCCGGCGCCGCGGGTGTGATCAAGATGGTCATGGCGCTGCGCCACGAGACGCTGCCGCGCTCTTTGCACATCGCGGAGCCGAGCCCGCATGTGGCGTGGGACTCCGGCGCGGTGGCGCTGCTGGACGAGGCCGTCGAGTGGCCGCGCGCCGCGCGGCCCCGGCGGGCGGGGGTGTCCGCCTTCGGCTTCTCGGGGACCAACGCGCACCTGATCCTGGAAGAGGCCCCCGAGCCCGGGCCTCCGGCACCCGAGCCCGCGCCGTCCGCGTCCGAGCCCGCGCCGTCCGCGTCCGAGCCCGCGCCCGTCACCCCCTGGGTGCTGTCGGCGCGCAGCGCGTCCGCGCTGCGCGCACAGGCCCGGCGGCTGCTCGACGCGACGCTCGACGCCGATCCGCGGGCCGTCGGCTGGTCGCTGGTGACCACGCGCTCGGTGTTCGAGCACCGAGCGGTCGTCACCGGCCCGGACACCGAGACACTGCGGGCGCGGCTCGGCGCGCTGGCGGCGGACGAGCCGGCGCCCGGAGTGGTGACCGGAGCACCGGGCCAGGCGGGCGCGGGAGCGGTCCTGGTGTTCCCCGGGCAGGGCTCCCAGTGGCCCGGGATGGGCGCGGAACTGCTCGGCGCCTCACCGGTGTTCGCCGCCCGGATCGCCGAGTGCGAGGCGGCGCTCGCCCCGTACGTGGACTGGTCCCTGACGGATGTGCTGCGCGGGGCCGACGGCGCGGCCGACCTCGGCCGCGTCGATGTGGTCCAGCCGGTGCTGTGGGCCCTGATGGTCTCCCTGGCCGCCCTGTGGTCCCACCACGGCGTGCGGCCCGCGGCCGTCGTCGGACACTCCCAGGGCGAGATCGCCGCCGCGTGCGTCGCGGGCGCGCTCACCCTCCAGGAGGGCGCCCGGGTGGTGGCGCTGCGCTCGCGGGCGCTGCGGGCGCTGGCCGGCCGGGGCGCGATGGCCTCCCTCGGCGTGGCCCCCGAGGCGGCCGAGCGCATCGTCTCCGACCTCGGCGAGGCAGCCTCGGGCGTGGGCGTGGCGGCGCTCAACAGCCCGTCCTCCACCGTGGTCTCCGGCCCGCCCGACGCGGTGGCCGCCGTCGTCGCCGCCTGCGAGGCCACCGGCGCCCGGGCCCGCGCCATCGACGTGGACTACGCCTCCCACGGCCCCCAGGTGGACGAGATCGCCGACGAGGTGACGGCCCGCCTCGCCGGGGTCGGCGGCGCGGCCACGGACGTCGCGTTCTGCTCGACCGTGACCGGCGGCCTGATGGACACCACCGCCCTGGACGCGGACTACTGGCTGACCAACCTGCGGCAGCCCGTCCGCCTCACCGAGGCGGTCGACACCCTGCTCGCCGCGGGACACCGGCTGTTCATCGAGGTCAGCACGCACCCCGTGGTCGTCCCCGCGCTCCAGCAGTGCTTCGAGTCAGCAACTGCCGGATCGCCCGTCGACGCGGTGGCGCTGGGCACGCTGCGCCGCCAGGAGGGCGGCCCGGCGCAGCTGGCCACCGCGCTGGCGCAGGC GTTCACCGCCGGGGCGCCCGTCGACTGGCGGCCCTGGTTCGCCGGCCCGTCCGCACCCGCGACGGTGTCCCTGCCCACCTATGCGTTCGACCGCGAGCGGTACTGGCTGCCCCCGGCCCGTGCGGCCCTGGGCGACGACACCCGGGACCCGGTCGAGGCCGAACTCTGGGACGCCATCGAGGACCGCGACATGGAGGCGCTGGGCCGGGTGCTGGAGCCGGACGGCGACGCCACCGCCCCCGGGGACATCGAGGCGCTGCGGCCCGCCCTGCCCGTGCTGTCCGCCTGGCGCCGCCGCCACCGCGAACGCGTCACGCTGGCCTCCTGGCGCCACCGGGTCCGGTGGACACCGCTGTCCGAGCCCGAGCCGCCCGCGCTGAGCGGCACCTGGCTGGTACTCGTCCCCGCCGGCCACGAGGACCACCCGGCGGTGCGGACCGCGACCCGGGCCCTGGCCGAGCACGGCGCCGACGCCACGGCATGGCCCCTCGACCCCGTGGCGGCCGGCCGCCACGCGCTCGCCGAACGGCTGGCCGCCCAAGGCCCCGACACCCCCCTCGCCGGGGTGCTCAGCCTGCTCGCCCTCGACGAGCGCCCGCACCCCGCACATCCGGCCCTGCCGGCCGGGCTCGCCGCCACCGTCGCCGCCGTCCAGGCACTCGGCGACGCGAAGATCACCGCGCCGCTGTGGTGCCTGACCCAGGGCGCGGTGTCCACCGGCCACGACGACCCGCTGACGCATCCGCTCCAGGCGCAGACCTGGGCCTTCGGCCTCGTGGCCGCGCTGGAACACCCCGACCGGTGGGGCGGCCTCGTGGACCTGCCCGCCGACCCCGACGAGCGGACCGCGCCCCGGCTGGCGGCCCTGCTGTCGGGCGCCCCGGGGCATGGCCGGGGCGAGGACCAGACGGCGATCCGTACCACCGGCGTGCTGGCGCGCCGCATCGTGCACGCCACCTCGGACCGCTCCACCGCGCGCCGGCCGTGGCAGCCGCGGGGCAGCGTGCTGATCACCGGTGGCACCGGAGGGGTCGGCGCGCTGCTGGCCCGCTGGGCGGCCGGACACGGGGCGGCCCATCTGCTGCTCACCAGCCGCCGCGGCCCCGACGCCCCCGGCGCCGGGGAACTCGCCGCCGAACTCCGCGCCCTGGGCGCCACGGTGACCGTCGCCGCCTGCGACGCCGCCGACCGCGAGGCCATGCGCTGCGTCCTGGAGGCGATCCCCGACGAGCACCCGCTGTCCACCGTCATCCACGCCGCCGGCGTCAGCGAGCAGGAGCTCATCGCGGACGTGGACGACGCGCATCTGGACCGTATGCTCGCCCCGAAGGCGCTCGCGGCCCGGCACCTGCACGAGCTGACCCAGCACCTGGACCTCTCGGCGTTCATCATGTTCTCCTCGGTCACCGCGTCCTGGGGCAGCGGCGGGCAGGCTGCCTACGCCGCCGCCAACGCCTACCTGGACGCCCTGGCCGAACACCGCCGCGGCCTCGGCCTGCCCGCCACCTCCGTGGCCTGGGGGCTGTGGGGCGCGGTGGGCATGGCCACCTCCGCCGAGGAGATCGACTTCTTCCGCCGCCGCGGTATCCACCCCCTCGACCCCGACCTGGCCGTCGCCTCGCTCCAGCAGGCGGTCGAGCGCCGGGAGGCCGGCGCCGTGATCGCCGCCGTCGACTGGCGCCGCTTCCTGGCGAGCTTCACCGCGCTGCGCCCCAGCCCCCTCCTGTCCGACCTCCCCGAGGCCGCGACGGCGCGGACCGAGGAGCCGTCCCGGGCGTCGGAGGAGGAGGCGGACCCGCTCCGGCGGAAGCTCGCCGGCAGTCCGCCGGCCGAGCGCCACCACATCCTGGTACGCCATGTGCAGGCGCACGCGGCCAGCACCCTGGGGCACGACGACGCGGACGCCGTCCCGCCCACCAAGCCCTTCCAGGAGCTGGGCTTCGACTCGATCACCGCCGTCCAGCTGCGCGACCGGCTCAACGCCGGCACCGGGCTGCGACTGCCCACGACGGTCCTGTTCGACTACCCATCGGCCGAGGAACTGGCCCGTCATCTGCACGGGCTGCTCGGCGCCGAGGCGGCCTCGGGCGAACAGCAGCTCATGTCGGAACTCGACGGCTGGGACGCCGGCCACGCGCCGGACGCCGTGGACGAGGCCGCCCGCGCCCGGATCGCCGCCAGGCTGCGGCTGCTGGCCGACAAGTGGGCAGACCCGGCCCGTACGGCCGGCGCCGCCGGGTCCCACCACGATCTGGAGACGGCCACGGCGGAGGAGATCTTCGACCTGATCACCACCGAATTCGGAAAGTCCTGAGCGGGGCCCGAAGAAGGGCCGAAGAAAAGAGGGGTTCGCCGGCGGTCCGGCGAACCCCTCGGGGGAATTCGGGGAAAACAGCGTCGATGCGTCAACGGGAGCCGTACTCGGCCACCCGCTTCTCCAGTTCGGGAACCACGTCATTGGGGGACGGCTGAGCCAGCATCTCCTGACGCAGCCCTTCCGCCCCGGACCGGAACGACGGATCGTCCAGCAGCCGGACCAGATCCTCCCGGAACCTCGGCCCGCTCATGGCCTCGGGCGGAATCCACAGCCCGGCCCGGACCTCCGCAAGACGCCCGCCCCGGGCCCGGGATTCCGGACCGCCGGGGCCGACCATGAGCTGCGGAACTCCGTACGCCGGCGCGCCGCAGAAGGCCGGGAGACCCCCGCGGTGCACCACCGCCGAGCAGGACGGGAGGAGGACGTGCAGCGGTGCGAAGTCGACGATTCTCGCGTGCGCGGGCACGCGCTGGAGCGTCCGCCGAATTCCCGCGGGGAGCGTCAGCACCAGCTCGATGTCGAGGTCGGCCGTCGCGTCCAGCATCTCCTGGGCCTCCTCGGCGGAAACGGGCCGGCCCCCGTTCCACTTGCTCACCCCGAAGGCCGCGAGGACGCGCGGCAGCTCCGCATCGCGGCGGGACCACTCCGGTACGACGGAAGGGCCGTCGTACGGGACGTAGCGCACGGACACGGTCCTTGGACCGCTCTCGGGCCGCAGGGATCCCGGTGTCTGATCGATGGTGAAACGGCCCGTCAGCGGCTCCTCCGGCGGGTCGCCGTATTCCTCGCCGAAGACCGGTCGCATCCGTGCGTGCGGTGCGCCCACCGCGGCGGCGGCGACCGCGCCCGCCTGACTCGCCGATTCCCCGATCACCAGATCGGGCCGCCACCAGCGGCAGTATTCCACCAGGTCCTCGGCGCTCGGCTGGACAGCGACCGCGGCCAGCCCCGCTCCGGTGACGACGTCGGCGAATTCGGGGGCGCTCGCGACATGAACCTCATGTCCCGCGGTCCGCAGTGCCCAGGCCAACGGCACATTGAGCTGGAAGTGGCTCCGCCCGGGAAAAGTGACGAACAGGACCCGCATGGCTGCCATCTCCTCGCCGTCGACCACGCCGCATCTCTCACCCGCTATCGTCCCGACACCTCTAAAGCGGTTCTTGCGAGCCCGCGCCGGCGCCCATTCGCGGTTAGTCATGCTTTAGACCGGTGGGTGACG ATGTCGCATATGATGAAAGGCATCGTTCTCGCGGGCGGGAACGGAACACGGCTGCAACCGCTCACCCTGGCGGGGTCCAAACAGTTGGTTCCGGTTTACGACAAGCCAATGATCTACTATCCGCTGTCCGTCCTCATGTTCGCCGGTATCCGGGACATTCTGATCATCAGCCGCCCCTCCGAATTGCCGCAGTTCCGCCGGCTTTTCGGGGACGGCGGCCATCTGGGGCTGAACATCTCGTACGCCTCCCAGGAGAAACCGCGGGGAATCGCCGACGCCTTCCGGGTCGGCGCGGATCACATTCGCGGTGAGGAGTGCGCCCTGATCCTGGGCGACAACCTCTTCCACGGCGCGAACCTGCCCGCGCTGCTCCGCAGGAGCGCCCAGCGGCTGCGCGGATGTGTGCTGTTCGGGCACGAGGTCGCCGATCCCCGGCACTTCGGCGTCGCCGAGATCGACGACCGGGGCGAGCTGCTGTCCATCGAGGAGAAGCCGGAGCGGCCCCGGTCCAACCTGGCCATTCCCGGCCTGTACTTCTTCGACGGCAGCGTCGTCGACATCGCCAAGCGCCTGGTCCCCTCGGCCCGGGGCGAACTGGAGATCACCGACGTTCTGCGGGCCTATCTGGAGGAGGGCAGGGCCGACCTGGTGTGGCTCGGCCGGGGCGTCACCTGGCTGGACACCGGCACCCACGAAACCCTCCTGGACGCGGGCCGCATGGTGCGCGACGTACAGCACTACCAGGGCACCCGGCTCGGCTGTGTCGAGGAGATCGCGATGTACATGGGATTCATCGGACCCGACGACTGTCATGCGCTCGGCGCGGAGATGAGCAATTCGCCCTACGGCCAGTACGTCATGGACCGGGCCCGCTCGTACCGGCAGGGCCTGCCGCCCGCGCGGTTCCTGGAGGAAATATGAGACTCCTCGTCACCGGGGCCGCGGGGTTCATCGGCTCCACCTACGTACGCGGGCTCCTCGCGCGGGACGGTGACCCGTGGGGCCCCGAGGTCTCCCATGTCACGGTGCTGGACAAGCTCACCTACGCCGGGACGCGGCGCAACCTCGACCTCGCCGACCCACAGCTGACGTTCGTCCACGGCGACATCTGCGACGCCGAGCTGGTCGACACCCTGATGGCGCGGTCCGGGCAGGTGGTCCACTTCGCCGCCGAGTCCCACGTCGACCGCTCCATCGACGGCGCCGACGCGTTCGTGCGCACCAACGTCCAGGGCACCCACACGCTGCTGCAGGCGGCCCTGCGGCACCGCGTGGAGCGCTTCGTCCACGTGTCCACGGACGAGGTCTACGGCTCCGTCGAGACCGGCTCCAGCCCCGAGACCGCCGTACTGGACCCCAACTCGCCCTACGCCGCCTCCAAGGCCGCGTCGGACCTGATCGCCCTCGCCTACCACCGCACCCATGGCCTGGACGTGCGCGTCACGCGGTGCAGCAACAACTACGGTCCCCACCAGTTCCCCGAAAAGATCATCCCGCTGTTCATCACCAACCTGCTCGACGGCGAGGACGTGCCGCTGTACGGCGACGGGCTGAACGTGCGCGACTGGCTGCACGTGGAGGACCACTGCCGCGGCGTGGAACTGGTGCGCGCCAAGGGGCGCCCCGGCGAGATCTACAACATCGGCGGCGGCACCGCGCTGACCAACCGGGAGCTGACCGGCCGGCTGCTGGAGGCCTGCGGCGCCGACTGGGGCCGCGTACGGTACGTCGCGGACCGCAAGGGACACGACCGGCGCTACGCGATCCGGGACGACAAGGCCAGGGCCGAACTCGGCTACCGCCCGCGCCACGACTTCGCCGCCGGGCTGGCCGAGACGGTCGCCTGGTACCGGGACAACCGCGGCTGGTGGGAACCGCTCAAGCACTCCGCCCAGCCGATACGCCGATGACCGCGCGGCGCCCGCGCGGCCATCGGCGTGCCGTGGCGCCGCTCAGCCGCGGTCCCCGACGAACTCGGTGATGGACGAGACCATGTAGTCGATCATCTCCTCGGTCAGCCCAGGGTAGACGCCGACCCAGAACGTGTGCTCGGTGATGATGTCGCTGTTGGTCAGCTCGCCGGACACCCGGTGCGGCGCCTCGGCGTGCGCGGGGTGACGGGTCAGGTTCCCGGCGAACAGCCGACGGGTGCCGATCTTGCGGGACTCCAGGAAGTCGACCAGCTCGGCGCGGCTGAACGGGGCCCGCGGGTCGACGGTCAGGACGAACCCGAACCAGCTCGGATCGGAGCGCGGCGTCGCCTCCGGCAGGATCAGCCACGGCAGCCCGTCAAGACCCTCGCGCAGCCGGCGCCAGTTGTGCCGCCGTGCCGAGCAGAATGCGTCGAGCTTGTCGAGCTGGGCCAGCCCCAGCGCCGCCTGCAGATCCGTCGACTTCAGGTTGTATCCGATGTGGGAGAAGATGTACTTGTGGTCGTAGCCGGCCGGCAGCGTGCCCATCTGGTAGCCGAACCGCTTGCGGCACGTATCGCTCTCCCCCGGCTCGCACCAGCAGTCCCGGCCCCAGTCCCGCAGCGACTCCACGATCCGCGCCAGCGCCAGGTTCGAGGTCAGCACGCAGCCGCCCTCGCCCATCGTCAGATGGTGTGCGGGATAGAAGCTGACGGTCGTCAGATCGCCGAAGGTGCCGGCGAGCTGCCCGTCGTAGGTGGAGCCCACGGCGTCGCAGGTGTCCTCGATCAGGACCAGATCGTGGTCCTGCGCGAGCTGGGCCAGCTCCGCGGCCTCGAAGGGGTTGCCCAGCGTATGGGCCATCATGATGGCCCGGGTGCGCGGCCCGATCGCCTGCGCCACCCGCTCGGCCGTGGCGTTGTACGTCCCCAGCTCGACGTCCACATAGACGGGGACCAGCCCGTTCTGCAGGATCGGGTTGACGGTGGTCGGGAAGCTCGCCGCCACCGTGATGACCTCGTCGCCGGGGCGCAGCCGGCGGTCGCCCAGCAGATGGGAGGTGAGCGAGGAGAGCGCCAGGAGGTTCGCGGACGACCCGGAGTTCGTCAGATGGGCCTTGCGCCGCTTCATCCTGCGCGCGAAGGCCGACTCGAACTTCCGGGATAACGCGCCCGCCGCGATCCGCATCTCCAGCGCGGCCGACACCAGCGCGACCCGGTCCTCCTCGTCCAGCACCGCACCCGAGGGCCAGATCTCGGTGACCCCGGGGATGAAGCCCCGCCCCTCCTGAGTGTCCTGGTGGTACTTGCGCACCTCGTCGAGGATGCGCGCGACCCGTTCGCTCATCTGCTGTCCTTCCCATGGCTGTTCCCGGGCCCGCATGCCGTCGCCGTACGCGCCGCCGCGGGCGGCGAGCCGGCGGCCGCGAGCAGATCACGCAGTGACTCGCGCAGGGTTCGGCGCGGCGCCCAGCCCAGCAGGCGCCGCGCCCGTCCGATGTCCAGTCGCTGCCAGGCCGCGTCGGAGCGCGTCCGCTGCAGGGCCGGGTCCTCGACGACGCGCACCGGCAGCCCGCTGAGCGAGATCATCAGGTCGACCAGCCGGCGCACGGGCACCGCCTGGCCGCTGCCGATGTTGACG ACGGTTCCGGCGACCGCGCGCACCGGCGCCACGGCCGCCGCGACCACCGCGTCGGCCACGTCCCGGACGTCCACCAGGTCCCGGTGGTCGCGCAGCGGCGCCAGCCGCAACTCGCCGCGCCCGGAGGCCAGATGGGCGGCCACGACCCCGGCGAGGCTGTTGCCCGGGGCGCCAGGACCGCAGGCGACCGAGATGCGCAGCACGGTCCCGGGCACGCCGCGCTCGGCCGCGGCCCGCAGCACGGCCTGCGAGCCGCGCAGTTTGGTGCGGCCGTACACGGTGGCCGGGTCGGTCGGCCAGTCCTCGCCGATCGAGGTCCCGGGCGCGGCCGGGCCGTACTCGTAGGCGCTGCCCAGATGGATCAGCCGCGGCCGGTCCGGCAGCCCGGCGACGACGGTGGCGAGCCGGGACACGAACGCGGAGTTGGCCTCCGTCATCTGCCGTTCGCCGCCGCCCCAGACCGCGCCCGAGGCGTTCACCACCACCCGCGCCCGCGTGTCGGAGCACAGCCGGGCCAGCTCGGCGGGGCCGGCCGCGCTCAGGTCCAGGCGTATGGAGCCGCAGTCGTCCCCGTCCGCGCCGTCCCCGTCCGCGCCGCCACCGCGCGTGCCGCGGCAGACCCGTGACACCCGGGCGCCCGTGGCGGCGAACGCCGCGCAGATGTGCCGCCCCAGGTATCCGGAGCCGCCCAGCACCAGGACCGGCGTGCCGGCCGGAGCGTCGTCAGCGCGGAGCGCGGGCGCCGGAGAGGCGGCCATGGTTCCCTCCCGGGAGGACGGGGCTACGGAAGGTCGCTGCCGGTCTTCTCGCCGCCTGTTTTCCGGAAGACAGCCTGCCAGAAGTAGCTCCAGGGAAGGCCCCGCGTATCGGCGGCGCAGGTCCATTTGTCGCCGGGCCGGTACGCGTCGACGAAGGCGGGCACGGCCGCCGCCACGGCCTCGCTGTCGTGGATGTCGAGCACCTGCCGCAGCGGTCCGCTCTCCATGGCCAGCCGCACGGCCTCCTCGCCCTTCTGGTGGCCGTCCATGCTCTTGTCCCGGAACTTCCCGACCGGGTTCTTCACGAAGAACGCCCCGCACCTCTCGTCGATCAGACGCAGATAGGCGGCGACGGTGTCGGGGGCCATCTCCGTGAAGGAGTGCACATTGACGCACAGCTCGAAGCGGTCCGCGCCCAGCGCGGTGTCGATGTCCTCCACCTGCACGAACTTCATCTTGGCGAACTGCTTCTCATCCAGGACGGCGCGCAGATACGCCCTGCTCAGCGCGAGCGTGTTCTTCAGATCGATGATGGTGTAGGAGGCGAGGTCGTAGTTGGACAGCATCGCGTGGCAGGTGCGGCCGTACCCCGCGCCGATTTCCAGGACACGGGCGCCGCCCAGGTCCAGCTCCTTCTCGATGAATCCGAGCTCGAGCGCCGCCTGGAGATAGTCCAGACAGATGCTCCTGCCCTCGTAGCGGACCGTGACGGGGTCTCCCACCTCACGGTTGCGGACATTCTCGATCTTCGACCAGTCGTCTTCGCTCAGCCGCGTGGCCAGCTCGTACACAAGGGCCCTCAAATACCTGACCCCATTGGCCGTCGGATCCCAGAGCGCGATATTGAAATTCCGCCGATCCGACTTGAAACCGGTCAGATCGGTGGCCGCGTCCTCGGTGACCCAGAGTTCGCTGATGCGCTCCCACTGCCGACTGGCCTGGAAACCCTGCACCATCTCGCTCTCCCACTCGTCGATGTTGCTCGGGTGACTGCCGGTACGCAGCTTCCCGTACGCCGCTAAAGGCGGACTGACATGGAGCGCCGGCCCGGAAAACGAGGCTGCCGGTGCGCAAGATCCGCTTTAGTGGAGGCGGCGACAATAAGGGCGGTCCCTCCCATGAGAGAGAGGATGGCGCCCATGCGCGTCCTGTTCGTCGCATTTCCCTGGCGAACTCATTTCTGGTTCACCGTGCCGATGGCCTGGGCGCTGCGCACGGCCGGCCACGAGGTCCATGTCGCGAGCAGCCGGGAATTCGCCGTCAATGTCACCGAAGCGGGGCTGACGGCGGTCCCGGTGGGATCGAAGGAACCCATCCTGCAAAGGGCGTACCGAGAGCAGAAGGAGGCGTTCGGGGACGGGAAGGGAATCGTCGACCCCATCGATCTGGGCGAGAACCGCGAAGAGCTGCTTCCGTGGAGCTATCTGAAGGCGCTGTGCTCCGGTGCCGTCGCGAGATCCAGGATCGTCAACGACGCGATGATCGACGATCTGGTCGCGTACTGCCGCTGGTGGAAACCCGATCTGGTCGTCTGGGAGTGGCTGACCAACGCGGGCGCGGTCGCCGCCTCGGCGGTCGGCGCGGCACACGCGCGGATGCCGATCGGCCTCGGACTGGACGTACGGCTCCGCCGGCACTTCCTGAAGACGATGGCGCGGCAGGACCCGGGGGAGCGGGTGGACCCCGTGCGCGAATGGCTCGGCGGATGGGGGGAGAAATACGGCTTCGATTTCTCCGAAGAGCTGCTGACCGGGCAGTTCACCATCGAACAGTGGCCGGATTCCATGAGGCTGGAGACCGGTGTGCCCCATCTCTCGACCCGCTATGTGCCGTACAACGGCCGAGCTCCCTACAGCGGCCGGTCCCTCGTGCCCGACTGGGTCCACCATGAGGGGAAGGCGCCCCGGGTGCTCGCCACATTCGGGACGAGCATGTGGGACATGACCAAGCCGCTGCTCTCCAGCGCCCAGCTGCAGAAGATGCTGGACTCCGTGGCCGGCCTGGACATCGAACTGGTCCTGACCCTGCCGGCCGGAATCCAGAAGGAGCTGGAGCGCGTACCGCGGAACACCAGGCTCGTGGAGTTCGTTCCCCTGCACGCCATCATCTCGTCCTGCTCGGCCGTGATCCATCACGGTGGATTTCCCACCTTCTGCAGCTCGCTGGCGCATGGGCTGCCCCAGCTCATGGTCAGCCGCAGCACCCCGGACGCCGCGCTGCGCGGCGCGCGCCTCGAAGAGGCCCAGGCAGGTCAGTGGCTTCCGCCCCACCGCACGACGGGCAAGGAAATCGGGAAATACCTGGCGCGGATGCTGGAAGACCCTTCGCTGCGGTCCGGCGCCGAACGGCTCCGTCAGGACGTACTGGCCCAGCCGTCGCCCAACCAGCTGGTGCCCGAACTCGAGCGGCTGGCCGCCGAATACCGCTCCGCTTGATCCCCCCTTGAACACCCCTGGTCGGTCAGCCGACCGCGGCCGCCCCGGGAGGGCGCGCGCGTCCGAACCCGCGCGCCCTCCCGGGGCGGTGTCACCGTCGCGGCGCCGCCCGCCCGCTCACCACGCGACCGGCAGCTGATGGACACCGTTGAGGTACAGGTCTTCCCTGGTCGGGACCTGCTCCGGCGGAACGGCCAGCCGCAGGCCCGGGAAGCGGTTCAGCAGCGCCGGGAAGGCCTGCCGCAGCTCGGTGCGGGCCAAGTGCTGGCCCAGGCACTGGTGGAAGCCGTGGCCGAAGGTGACATGGCCGCCGACGGGCCTGGTGATGTCGAGGGTGTCGGGGTCCTCGAAGC GCGCCGGGTCACGGTTGGCCGTCGGCAGCGACACCGTCACCGACTGACCCTTCCTGATCATCGTCCCCTCGACCTCGACATCCTCCAATGCCGTGCGCACGGGACCGAACTGGCCGACCGTCGCATAGCGCAGGAGCTCTTCCACCGCGGTGTCGACAAGACCCGGGTCGCGGCGCAGCGCCTCCATCTGTTCGGGGTGGCACAGCAGGGCGAAGGTGCCCAGCGACAGCGTGTTCGCGGTGGTGTGGAGGCTGCTCGCGAACAGGAGGAGGCCGATGGTGGCCAGCTCCCCCTCCGTCAGCTGACCGCCGTTGACCAGACCGCTGAAGAGGTCGTCGGTGGGTTCCGCGCGGCGGCGCTCGATGAGCGGCGGGAAGAAGCTGAGCGTGACGGTCAGGGCCGACTCCGCCTTCTTCGGCTCGGTGTCCAGGGCGAACAGGGTGTCGATCGCCTGCCGGAGTTCCTCCCGGCCTTCCTCGGGGATGCCCAGCAGTTCACAGATCATCAGATACGGAATGGGCGCGGCGAACTCGGCCATGAGGTCCGCCGGCGGGCCGGAAGCCTCCATCGCGTCGAGACGTCCGTGCGTGATCGCGGCGATCGCCGTCTCAAGCCGTTTCATCCGCCGAGTGGAGAACTCGGCCATCAAAAGACGCCGGTACCGGGTGTGTTCCGGGGGGTCCAACAGCGGGAGCATGCCGGGACTGCTGATTCCCATGCCGGAGATGAACTCGGAGGCCGAGGGGATGAACGTCCGGGTGTCCAGCTCCTGCCGGGCGCTGAACCGGGCGTCGCCCAGAATGGCCCGCACCGCCGAGTGCCCGGTGGCCAGCCAGCCCCAATGGCCGTCCGCGAATCGCATGGGCGAGATCGGCCGCTCCTCCCGCATGCGCTTGAGCTCATCGGGCGGGTCGAAGGGACGGGGGCGGCCCTGCCGGGAAAAGTTGATCGGGGTGTTCTGCATGACTCCTCCTCAGAATTGTCGCGCGGTCATCACGAAACTCTCCCTTTCGCCCGCAACAGGTCCCTTTCCGTAGATGATCGGCGAACCGCCTAAAGCGGAACTGTCCACCAGGGCGCGCCCCGGCGGCGCGGGAAACTGCGGGCGACGGGCTCGGCCACTGCTCAGGACTCCCCGAACTCCCGGTCGATCAGGGCGAACATCTCGTCCGCGGACGCCGCTTCCAGGTCGTCCCCGCCCGTCGCCTCGGCAGGCTCGGGCCCACCGTCCCCGGCGCCCTGCCCCTCGTCGTATCGCCACAGCAGCACCCGCAGCCGGTGCGTCAGCCCGGAGCGGCGCGGATCGTCCGCGTCCAGGGCGGCGAGCGCCGCCTCGATCTCGTCCAGGTGCGCCGCGACCGGCTCCGCCTCCGGCTCCTCGGGCAGCACCTGCGTGACCAGATGGCCGGCCAGCGCGGTCGGCGTCGGATAGTCGAAGACCAGCGTGGCGGGCAGGCTCAGGCCGATGGCGCCGCGCAGCCGGTTCTTGAACTCGACGGCCGCCAGGGAGTCGAAACCCAGCTCCTTGAAGGGCTGGTCCGGGTCGACCGACTCCGCGGCCGGGTAGCGCAGCGCACCCGCCACATGGGAGCGCACCAGCGCCAGCAGCGTGCCGGCGCGGGCCTCGGGCGCCTGGGCCGCCAGCCGCTCCCGCAGGGCCGTCAGGCTCTCGGAACCCTCCTCGTCCGGGGCGTCGAGCGCCGCCTGCCAGGCCCGCCGGGCCGCCGGTATCTCGTCGAAGAGACGGCTGGGGCGCGCCATGGTGAACAGCGGGACGAAACGCTCCCAGTTGATGTCCGCGACCACCGCGTGCGCGTCGCCCCGCCCTTCACGGGTGCCCTCGGCGTCGCACTGCTCGCCGCCGTGACCGCCCTCGTCGAGGGTGCGCCGCAGCGCGGCGAGCGCCAGCTCGGGCGGGAGGGCCGGCAGCCCCAGCCGTTCGGCGCGCTCGGCCGCCTCCGGGCCCTCCGAACCGTCCCACACGCCCCACGCCAGCCGGACGACATGGCCGCCGGCCGCCTGACGGCGCCGCGCCACCGCGTCCAGATACGCGTCGGCCGCAGCCCGGGCGCCATGCCCGCCACCGCCCCAGACCGCGGCGATGGACGAGAAGACCACCACCGGATCGTCGGGCTCCAGGCCGCACAGCTCCGCGAGCCGGTCGGCTCCCCCCATGGCGTTCGACACCGCCTCGGCCAGCCCGTCCGGCGTGAACTCCGCCAGCGGCGCCCAGTCGCTCGGCGCCCCGGTGTGGACGACCGTACGGACCCGCAGCCCCGAGGCCGCGAGCCGGTCGGCCAGCTGCCGCCGCGCCATGGGTTCGGACGGCACGCCGTCCACCACCGTCGCCTCCGCGCCCAGCTCCGCCAGTTCGGCCACCAGCTCCGCGCCACCGGGCGTGGCCGGGCCGTCCGGCGCCAGCAGGACGAGGCGCTTGGTGCCGCCCGCGGCCAGCCACCGCGCCACGAGAGCCGCGGTCCCGGCGACCCCGCCGCTGACCAGCACACCGTCCCGGGAAGTCCACTCACGCGCCGGCGCCTCCCCGAGGCCGCTCAGTGGTGCGGGCAGCAGCCGCCGCCCGAAGGCCCCGGCCGGCCGCAGCGCCACCTGGTCCTCCCCGGCCCGCCCCGCCAGCACGGCGCGCAGCAGATCGGGGGCGAGGTCCTCGGGCGAGGCGGGCAGGTCGACCAGCCCGCCCCACGCCGCCGGATGCTCCAGCGCGGCCACCCGGCCGAGCCCCCACACCTGGGCCTGCGCGGGCGCCTGTGACCCTTCGCCGTCCGCGACGGCCACCGCGCCCCGCGTGGCGCACCACAGGGGCGCGTCGAGCCCCGCCTCCAGCAGGGCCCGCAGCAGCACATGGGTGCCCGTCACACCCCCGATCACGGCCGGGAACGCTTCGTGTGGGCGTTCGTCCAGCGCCAGCAGGGACAGCACCCCCGCCGGGGCCCCGCCCTCGTCGCACAGCACCCGCAGCGACGCGGCGAGCCCGGCCACCTCCGCCCGGCCGTCCGTCACCAGCCGCCACACCGCACCGCCGTCCGCCGTGAGGGCCCGCTCACAGGCGTCGGCCCACTCCTCGGCCTCCCCGGCGCCCCGGGGGGGTACGACCAGCAGCCACGGGCCCGCCGTGGCCGCCGGGCCGAGCTCCGGCAGCCGGCGCCAGCCGACCCGGTAGCGCCAGGAGCTCAGGGTCGACCGCTCATGGCGCTCCCGGTGCCAGCGCGACAGCGTCGGCAGCACCGCGCCGAGCGACGAGCGCCGCCCGTCCTCCTCGCCGATGCCCAGCGCCTCGGACAGCGCCGGGAGGTCCTGCGCCTCCACCGCGCTCCAGAACCGGACCTCCTCCTCGTCCGCCTCCGCCGGCGGCGCGTCCGCCAGGGCGTCCGGCAGCCAGTACCGCTCGCCCTGGAAGGGGTAGGTGGGCAGGTCCACGGTCGGCGGCGGGGCACCGTCGGTGAACCACGGGCGCCAGTCGACGGGGGTGAGGGTGTGCAGCCGGGCGAGCGCGTGGGCCAGGCCCTCGACGT CGGGCTGCTTGCCGGTCAGGGCCGGGACCAGCAGGGGAGGGTGCGCGGGCAGGCCGTCCAGCGCGTCGAGGGTGTTCTGCGCGGCCGGGATCAGTACGGCGTCGGGGCCGACTTCGAGGAAGGCCGCGGTGTGCGGGGCGATGTGTGTGACGGCGGGGTGGAAGTGGACGGGTTGGCGGATGTGGCGCACCCAGTAGTCGGGGGTGGTGATGTCCTGGCCGGCTGGTTCGCCGGTGAGGTTGCTGATGAGGGGGATGGTGGGCTGGTGGTAGGTCAGGCCGGCGATGGCGTCGCGGAAGTCGTCCAGGATCGGTTCGACCAGGGGGGAGTGGAAGGCGTGGCTGACGGTGAGGGCTTTGGTTCGGCGTCCGCGTTCCTTCCAGGCCGTGCCGATTTCGGTGACGAGGTCGGCCGGTCCGGAGATGACGGTGTTGGTGGGGGCGTTGAGGGCGGCGATGGTGACCTGGCCGTCGTACGGGGCGAGGGCTTCGGTGGTTTCTTCTTCTGTGGCTTCGAGAGCGGTCATGGCGCCGCCCGGGGGCAGGCTGCCGAGGAGGGTGGCTCGGGCGGCGACGAGGTGGCAGGCGTCTTGGAGGGAGAAGACTCCGGCGATGTGGGCTGCGGCGATCTCGCCGATGGAGTGGCCGATCACCGCGTCCGGGCGGGTGCCGCAGGTCTTCAGCAGCCGGGCCAGTGCGACCTGCACGGCGAACAGCCCGGCCTGGGTGTAGGTGGTGTGGTTCAGCAGGTCCGCTCGCGGCCGGCCGCGCAGTACGACATCGGCCAGCGGGTGGGCCAGCTGCGGATCGAGCAGCGCGCACACCTCGTCGAAGGCCTCGGCGAAGACCGGGAAGCGCCGGTAGAGGTCGGCGCCCATGCCCGGCCGCTGGCTGCCCTGCCCGGAGAAGAGGAACACCCGCCACCCGGAGCCCCGCACCCCCGCCTCGCGCCGTACCAGCGCCGGATGGTCCTCCCCGGCCGCCAGCGCGGCCAGGCCCGCCCGCAGCGCCGCGGTGTCCCGGCCCATGACCACGGCCCGGTGGTCGAAGACCGTGCGCGAGGTGGCCAGCGACCAGCCCACCGCGTCCGGATCGCCGTCGAGAGCCGCCGCGTCCAGCAGCCGCCCGGCCTGGGCGCGCAGCGCGTCCGCGCCGCGCGCCGAGACCACCCACGGCGTGACCCGGTCCTCGGGCCGTTCGCCGGCCGCGTCCGGGGGAGTGCCGGCGGGCTGGGTGTTCTCCTCCAGGATGACGTGGGCGTTGGTGCCGGAGATGCCGAACGAGGACACACCGGCCCGGCGCGGACGGTCCCCCCGCGGCCAGTCGACCGGCTCGGTCAGCAGCCGTACGGCGCCGGAGTCCCAATCCACATGGGAGGTCGGCTCGTCGATGTGCAAGGTGGCGGGCAGCCGGCCGTGGCGCAGGGCCATCACCATCTTGATCACGCCCGCCACGCCCGCCGCCGCGTGGGTGTGCCCGATGTTCGACTTCACCGACCCCAGCCACAGCGGCCGGTCCGCCGGGCGGTCCTGGCCGTAGGTGGCAAGCAGCGCCTGGGCCTCGATCGGGTCGCCGAGCGTGGTGCCCGTGCCGTGCGCCTCCACCGCGTCCACCTGGTCGGCGGTGAGCCGGGCGCTGTCCAGCGCCTGCCGGATCACACGTTCCTGGGCGGCGTCGTTGGGCGCGGACAGCCCGTTGCTCGCGCCGTCCTGGTTGACCGCCGAGCCGCGTAGCACGGCGAGCACGCGATGGCCGTTGCGCCGGGCGTCCGACAGCCGCTCCAGCAGCACCAGGCCCACGCCCTCGGAGAAACCGGTGCCGTCCGCGGCCGCGGCGAACGCCTTGCAGCGGGCGTCCGGCGCGAACCCCCGCTGCCGGGAGAACTCCACATACGCGGTGGGAGTGCTGAACACGGTCACACCGCCGGCCAGGGCCAGGTCGCACTCGCCCTGCCGCAGCGCCTGCCCGGCGAGGTGCATCGCCACCAGGGACGCCGAGCACGCCGTGTCCAGGGTGACCGCCGGGCCCTCCAGACCCAGCGTGTACGCCACGCGGCCGGACACCACGCTGGTCAGTCCGCCCGTGGCCGTATAGCCCTCGAACCCCTCGGGGATCCGCGGTATCCGCGACAGGTAGTCCTGGCTCGACACCCCCGTGTAGACGCCGGTGGCGGAGCCCTTGAGGCTGGTGGGGTCGATCCGCGCGCGCTCCAGCAGCTCCCAGGCGGTCTCCAGCAGGACGCGCTGCTGGGGGTCCATGGCCAGGGCCTCGCGGGGGCTGATGTTGAAGAAGGAGGCGTCGAACCGGTCGGCGTCGTGCAGGAAGCCGCCCTCGGTCACATAGCTGGTGCCGTAGTGGTCGGGGTCGGGGTGGTACAGGCGGTCGAGGTCCCAGACCCGGTTGGTGGGGAAGGGGCCGATGGCGTCGGTGCCGTCGGCCACCAGCTCCCACAGGGCTTCCGGGGAGGTGACCCCACCCGGGTAGCGGCAGGCCATGGCGGTGATCGCGACGGGCTCCTGGTGGCGCTCTTCCGCTTCGCGCAGCCGCTGTTTGGCCTGGCCGAGGTCGAAGGTGACGCGCTTGAGGTACTGGCGCAGCTTGTCTTCGGTGGTGGACATGGCTTCTCCCTCGCTGCCGGTCAGGACGAGCCCAGTTGCTGGTCGATGAGCGCGAACATCTCGTCGTCCGAGGCCGACTCCAGCGCCTCGCCGTCGACGGTGTGATCGACCCCCAAGGCGCCGTCGCCCAGCCGCCACAACAGCGACTGCAGCCGTTTGACGAGCTGGGCACCGGTCTCGCCCTCCGGCTTCACCTCGCCCACCGCCGCCTCCAGCCGCTCCAGCTCCCGCAGCACGGGCCGCAGCGCGTCGTCCTCCGCCGGGCACAGCTCGCCCCGCAGGTGCTCGGCCATGGCGGCGGGCGTCGGGTGGCGGAACACCGACGCGGCGGACAGCCGCAGCCCCGTCGCCATGGTGAGCCGGTTGCGCAGTTCGACCGCCGTCAGCGAGTCGAACCCGAGATCCTTGAACGGCGTGTCCATCGCCACCGCCCCGGGGTCCGCGTGCCCCAGCACGGTGGCCGCGTGGGTGCGGACCAGATCGAGCAGGAGCGCCAGCTGTTCGTCGGCGCCCATCGACCCCAGTCGGCCGGACAGGTCGGCCACCGGCGCCGCCACGGACCGCCGTACGACCCGGGCCCCGGCGCTCGTGAGCGAACGGACCAGCGGCGGCAGCGCCTCGGCGGGCTGGGCGGACAGCGCCGCCAGGTCGAGCTGGGCGGCCAGCAGCCGCGCCATGCCGTGTGCGTGTGCCGCGTCGAACAGCGCCAGGGCGTGGTCGGTGGCCAGCGGCACCATCCCGGACCGGCTCATCCTGGCGTGGTCGACCTCGGTGAGCGCTCCGGTCATGCCGCTGGTGGCGGCCCACAGTCCCCAGGCGATGGAGGTGGCGGGCAGTCCCGCGTGGTGCCTTTGGGTGGCGAGAGCGTCGCAGTAGGCGTTGGCGGCGGCGTAGTTGGCCTGCCCGGGGCTGCC GAGGGTGCCGGCGGCCGAGGAGAAGATCACGAACATGGCCAGGGGGAGGTCCTGGGTGGCCCGGTGGAGGTTGGCCGCGCTCGTCGCCTTGGCCGCCCAGACGCGGCGGAGGGTGTCGGGGGTCTGAGAGGTGAGCACGGCGTCCTCGACGGTGCCGGCGGTGTGGACGACGGCGGTGAGGGGGTGCTCGGGGTCGATGGTGTCGAGGGCCTGCCCAAGGGCCTGGGGATCGGTGGTGTCGGTGGCGGTGATGCGGACGGTGGCGCCGAGCTCGGTGAGCCGGGCGGTCAGGTCGGCTGCGCCGGGGGCGTCCGGGCCCCGGCGACTGAGGAGGTGGAGGTGGGTGATGTGGTGGGTGGTGACGAGGTGTTCGGCTGTCAGGGCGCCGAGGGTGCCGGTGCCGCCGGTGATCAGGACGGTGCCGTCGGGGTCGAGGGCGGGGGGAATGTCCAGGACGATCTTGCCGGTGTGTTTGGCCTGGCTCATGTGGCGCAGCGCCTGGCGGGCCCTGGCCAGCGGCCACGCCTGCACCGGGGGCGGCGCCAGGACGCCCCGCGCGAAGAGGTCGGCGAGCGTGTCCAGCATCTCCGAGATCCGGTCGGGGCCGGCGTCGGAGACCAGGTCGTACGCGCGGTAGTGGACGCCGGGATGGGCGTCGGCGATCTGCTCGGGGTCGCGGATGTCGGTCTTGCCCATCTCGATGAACCGGCCACCGGGGCCCAGCAGCCGCAGCGAGGCGTCCACGAACTCGCCCGCCAGGCAGTCAAGCACCACGTCCATGCCCTCGGGCCCGGTGGCGGTGCGGAAGGCTTCCTCGAAGTCGAGGTCGCGGGAGGAGGCGCGGTGGGGCTCGTCGATGCCCATGGCCCCCAGCGCGGCGTGTTTGCCGGGGCTGGCCGTGGCGTACACCTCGGCACCCAGGTGGCGGGCGATCTGCACGGCCGCCGTGCCCACGCCGCCCGTGGCGGCATGGATGAGCACCGACTCGCCCGCCCTGAGGCCCGCGAGGTCCACCAGTCCGTACCAGGCGGTCAGATACGCGATGGGCACGGCCGCGGCCTGCCGGTCGGTCCAGCCCGGCGGGATCGGGACGACGGCGCGCGCGTCGGCGAGGGCCACGGGCCCGAACGAGCCCTGGAACAGGCCCATGACGCGGTCGCCGACGGCCACCGACGTGACGTCCCCGGCCACCTCCAGCACCACGCCCGCGCCCTCGCCGCCCAGCCCGCGCCGGTCGGGCACGACGCCCAGGGTCACCATCACATCGCGGAAGTTGACGCCCGCGGCGCGGACCGCGATGCGCACCTGCCCGGGCGCCAGCGGCTCCAGCACCTCGGGGCACTCCTCCGGCGCCACCCCGTCGACCGTTCCGGCGTGCTCGTCCGCCATGACGAGTTGCCAGGCGGACGAGGTCACGGGCAGCACGATGTCGGCGGTCGCGTCCGCGGCCGTCAGCCGCGGCACCCACGCCGCCCCGCCGCGCACGGCCACCTGCCACTCGTCCGCGGCGAGGGCCGCGCCCAGCACCCCCACGACATCCGGTGCCGGACCGGCGTCGCGGCCGCTGAGGTCGAGCAGCGCGAACCGGCCGGGGTGCTCCGCCTCGGCGCTGCGGACCAGCCCCCATACGCCCGCGGCCACCGGATCCACGGCGCCGCCCTCCGGGCCGGCGGGGGTGTCCTGCGCCACGGCGGCGGCGTCATGGGTGACGACCACCAGCCGGGAGCCGGCGAGCCGCGGCTCGGCCAGCCACTCCTGCGCCGTCGTCAGAACCCGCGCCACCACATCGGAGCCGTCGCCGTCGCTGCCGCCCTCGGCGGGTGCCGGGCCGGTCAGGACGGTCTGCGGCACGGGCGCGCCGTCGTCCATGGCCGCGATCAGCGCGGCGACATCCGGATACCGGGACAGCGACGGCACCGCCTCGTCGAGCCGGCCGTCCCCGAGCACCGCCCAGCCGTCCGCTTCGGCCGTCGGCGGCTCCGCCGGGATCGGGAGCGCGATCCACCGCACCTGGTACAGACCGTCGTTCGGCCCGCTGGTCCGCAGGCGCGCGGTGTCGATGGGCCGGGTGGTGATGGTGTCGGCGGTCAGAACGGGCCGCCCGGTGGTGTCCAGGAGGGCCAGGCCGAGCTCGCTGTCGCCACGGCGGGTGATCCGGACGCGGGCCCGCGTGGCGCCGGTGGCGTGCAGGGTGACACCGCCCCAGCTGAAGGGGAGCCAGATCTCGCCGGCCGGGTTGTCGGCGGTGGCGAGGAGCGGGTGGAGCGCGGCGTCGAGGAGGGCGGGGTGGAGGGCGAGGGGATCGGCGGTGGGGTCGGTGGCGTCGGCGGCGGGGAGCTCGATGTCCGCGAGGAGGTCGTCGCCGTGGCGCCAGACGGCCCGCAGGCCCTGGTAGCTGGGTCCGTAGTGGTAGCCGCCCGCGGCGGCGCGGTCGTAGAAGCCGGTGATGTCGACGGGTTCGGCTCCCGGGGGTGGCCAGGCCGCCTCCGCTCCGTCCCGGGGCGGCGCGGCCGTGGGCTCGTCGGTGAGGGTGGCGGCGGCGTGGTGGGTCCACTCCGCCGTGGGGCCGGACTGCGAGCGGGCGTAGATGTCCAGGGTGCGGTGACCGGTGTCGTCGGGTGGACTGACGATGACCTGGAGTGAGAGCGGGTGCGTGGGGTCGAGCGTGAGGGGCTGTTGGAGGAGGAGTTCGTCGATGTGGGGGGTGGCGGCGTGGTCCGCGGCGTGCAGGGCGATGTCGAGGAGGGCGGTGGCGGGCAGGAGGACCGCGCCGGCGATGGTGTGCTCGCTGAGCCAGCCCTCGTGGCCGGCGGCGGGCACCTGCCCGGTCAGGAGGATGCCGCCGTCGGCGAGCGGCGCGGTGGCGGCGAGGAGGGGGTGGGTGAGCGAGGCGGCGCCGGGGGTGGCCGGCCCCGCGTGGGTCGGGGTCAGCCAGTAGGGCCGGCGCTGGAAGGGGTAGGTGGGCAGGTCGACGCCAGGGGGCGTGGGGCCGGCGGGGTACCAGGGGGCCCAGTCGACGGGGGCGTGGGTGTGCAGCCGGGCCAGGGCATGGGCCAGGGCGCGGATGTCGGGCTGGCCGCGGGTCAGGGTCGGGATCAGCAGGGGTGGGTGTGCGGGCAGGTCGTCCAGCGCGTCGAGGGCGTCCTGGGCTGCGGGGACGAGGACGGCGTCGGGGCCGACTTCGAGGAAGGCGGCGGTGTGCGGGGCGATGTGTGTGATGGCGGGGTGGAAGCGGACGGGTTGGCGGATGTGGCGCACCCAGTAGTCGGGGGTGGTGATGGTCTCGTCGGCCGGTTCGCCGGTGACGTTGCTGATGAGGGAGACGCTGGGCTGGTGGTACGTCAGCTCGGCGACGGCGTCGCGGAAGTCGTCCAGGACCGGTTCGACCAGGGGGGAGTGGAAGGCGTGGCTGACGGTCAGCGCCTTGGTCCTGCGTCCGCGTTCCTTCCACGCCGTGCCGATCTTGGTCACCAGGTCCAGTGGGCCGGAGATGACGGTGCTGGTGGGGGCGTTGAGG GCGGCGATGGCGACCTGGCCGTCGTAGGGGGCGAGGGCTTCGGCGGCCTCCTCGGCGGTGGCTTCGAGGGCGGTCATGGCGCCGCCGGGGGGCAGACTGCCCAGGAGGGTGGCGCGGGCGGCGACCAGGCGGCAGGCGTCTTCGAGGGAGAAGACTCCGGCGATATGGGCGGCGGTGATCTCGCCGATCGAGTGGCCGATCACCGCATCCGGCCGCACACCGCACTCCTTCAGCAGCCGGGCCAGTGCCACCTGCACCGCGAAAAGACCGGCCTGGGTGTAGGTGGTGTGGTGGATGAGGTCGGCGGTCTCGTCGCCGGTGGCGAAGACGATGTCCCGCAGCGGGTGGTCCAGGTGCGGATCGAGCAGCGCGCATACTTCGTCGAAGGCCTCGGCGAAGACCGGGAATCGCCCGTGCAGGTCGGCGCCCATGCCGGGGCGCTGGCTGCCCTGTCCGGAGAACAGCCATACGGTCCGGCCTGATGTGGGCGTGGCGCCTGGCGCGGCGGTGATCAGCCCGGGGTGGGGGCGCCCCTCGGCCAGGGCGTCCAGGGCGGCCGTCATCTCCTGCTGGTCGCTGCCGACGATGACCGCCCGTCGTTCGAACACCGAACGCGTCGCGGCCAAGGCCCGCCCGACGCCCGGGACCGACAGGGGCTCGGCGGGCCGGCCATCGGCTCGTAGCCGCTCGGCGAGCGCCGTGGCCTGGGCGCGCAGGGCGTCATCGCTCCGGGCGGACAGCGGCCAGGGGACGATGCCGGGCGGGGGAGTGTCGGCCACCGGCTGGTGTGTGTGCGGCGCTTGTTCGAGGATGAGGTGGGCGTTGGTGCCGGAGGCGCCGAAGGAGGAGACGGCGGCGCGGCGGGGGCGGCCGGTCTCGGGCCAGTCGGTGGCCTCGGTGAGGAGTTGGACGTTTCCGCTGGTCCAGTCGATGTGCGGGCTGGGTTCGTCGATGTGGAGGCTGGCGGGCAGCCGTCCGTGGCGCATCGCCATGATCATCTTGATGGCGCCCGCGACACCGGCGGCGGCCTGGGTGTGCCCGATGTTCGATTTCACAGAACCCAGCCACAGTGGCCGGTCGTCGGGGCGGTCCTGGCCGTAGGTGGCCAGCAGCGCCTGGGCCTCGATGGGGTCGCCCAGCGTCGTACCCGTGCCATGGGCTTCCACGGCGTCCACATCCGCCGGGGTGAGCCCCGCGCCGGCCAGGGCGTGGCGGATGACCCGCTGCTGGGAGGGGCCGTTGGGGGCGGTCAGCCCATTGCTCGCGCCGTCCTGGTTGACCGCCGAACCCCGGATGACGGCCAGGATGACCCGCCCGTTGCGCCGGGCGTCCGACAGCCGCTCCAGCAGCACCAGGCCGACGCCCTCACCGAAGCCCGTGCCGTCGGCGGCGGCGGCGAACGGCTTGCACCGCCCGTCGGGGGCCAGCCCGCGCTGCCGCGAGAAGCCGGTGAACACATCGGGGGTGGCCAGTACCGTCACCCCGCCCGCCAGCGCCAGATCGCATTCGCCCTGCCGCAGCGCCTGGGCGGCCAGATGCATGGTGACCAGCGACGACGAGCAGGCGGTGTCCACGGTGACCGCCGGGCCCTGGAGGCCGAGGGTGTAGGCCACCCGGCCGGAGATGATGCTGCTGGTGCCGGCGAGATTGCCGTAGCCGTCCAGCCGGGGGTCCCCGGCGGGCAGACCACTGCCGTAGTCGTGGTACATGACGCCGGTGTAGACGCCGGTCAGGCTGTCCTTGAGGGTGTGCGGGTCGATACCCCCGCGCTCCAGGAGCTCCCAGGCGGTCTCCAGCAGGACGCGCTGCTGGGGGTCCATGGCCTGGGCCTCGCGGGGGTTGATGCCGAAGAACGCGGCGTCGAAGCCGTCGGCGTCGTAGACGAACGCGCCCTGGGAGGCGTAGGTCGTGCCCGGGTGTTCCGGGTCGGGGTGGAACAGGTTGGCCAGGTCCCAGCCGCGGTTGGTGGGGAAGGGCCCGAGTACGTGCTGTCCCGCCGCGACCAGGTCCCACAGCCCCTCGGGCGAGCTCACGCCACCGGGGAAGCGGCACGCCATCGCCACCACCGCGACCGGCTCGTCGGCGGCCCGGGCCCTGGACGGCGCGGCACCGCGGGGCGCGGGCGCGGCGGTGGCGGTCAGCCGCTCCAACAGGTGGTCGGCGAGGGCCTGGGGGGTGGGATAGTCGAAGACCAGGGTGGCGGGCAGCCGCAGCCCGGTGGCGCCTGCCAGGCGGTTGCGCAGCTCGACGGCGGTCAGCGAGTCGAAGCCCAGCTCCTTGAACGCGGCCTCGGCGCGGATCGCCGCGTCCGAGGAGTGGCCCAGCACCACCATGACCATCTGGCGCACCGTATGGAGCACCAGATCGCGCCGGGCGGTGTCGTCGAGACCGGCCAGCCGGCCCGCCAGCTTGGGGCCGGTGGCGTGGTGTTCCATGGCGCGGCGGCGGGCCGGCCTGGCCAGGGCGCGCAGCATGGCGGGGGCGTCGGGGCCGAGCCGGGTGGCGTCGAGGTCGGCGGCCACCACCAGCGGGTGGGCGACATGGAGCGCCGCGTCGAGGAGCCCGAGGCCACGGGACGTGGCGAGGGGACGCATGCCGAGGGTGCCCAGACGGGTCAGATCGGCCTCGGTCAGGTGCTCGGTCATGGCCGAGGTCTGCTGCCACAGGGTCCACGCGATCGAGGTGCCGGGCAGCCCGGCCGCGCGGCGGTCGGCGATCAGGGCGTCGGTGTACGCGTTGGCGGCGGCGTATCCGGCCTGGCCGGAGTTGCCGACGACGCCGGCGGCCGAGGAGAACACCATGAACAGCCGCAGCGGCAGATCACGGGTGAGAGTGTGCAGGTTCCACGCGCCGAGGGCCTTGGGGGTCCAGACGCGGGCGAGCCGGCCGGTGTCCCAGGACTCCACCAGGGCGTCGTCGATCACACCGGCGGCGTGCACCACACCCACCAGCGGGTGGTCCTCGGGGACCGCCGCCAGCACCTCGGCCAGCGCGGCGCGGTCGGCCACGTCGACCGCCGCCACCTCCGCCTCGGCCCCCAAGTCGACCAGCCGCCGCACGAGTTCGGCCGCGCCGGGCGCCTGGGCGCCGCGCCGGGACAGGAGCAGCACACGCTCCACCCCATGCGTACGCACCAGGTGTTCGGCGACCTCACCGCCCAGCACCCCCGTCCCGCCCGACACCACCACCGTGCCACCGGACAGCTCCGGGGCCTCGTCCTCGGCCGACGGAGAGGTGCGCACCAGGCGCGGCACCAGCGCTCCGGCCGCGCGCACGACCACCTGCGGCTCGTCGGCGTCGAGAGACCGGCGCACCGCCTCGGCCAGGAGCCGGTCCCCGTCCGCCGCACCGGCCGTGTCGACCAGGGTGAACCGGCCCGGGTGCTCGGCCTGGACCGAACGGACCAGACCCCACACGGCCGCGCCGCCCGGATCCACCGACCCCTCGTCGTCGCCGGCCGACACGGCGCCCGAGGTGACCAGGACCAGCCGGGAGTCCGCCAACCGCGGCTCCGCCAGCCACCCCT GGACGAGGCTCAGCACGCCCCCGACGGCGGCCAGGCCGCCGTCGGGGGCGGCAGGCGCTGTCACCGGGTGGAGGACGACCGTCGGCACCGGGGTCCCGTCGTCCACCGCGGCGAGCAGCGCGCCGAGGTCGGCGTGGGCCGCCACCCCGGCGAGTACGCCACCGTCGCCCGCTGCTCCCCAGCCGTCGCCCATGATCGCCCAGCCGTCGTCCGGCAGCGCCGGCGCGTCGCCCGTCCCGCCACGCGCGGGCACCCAGTCCACGACGAACAGGCCCTCGGCCGCGCGCTCGCCGATGGCGCGCAGCTCGGCGACGCCGGTGGGGCGTACGGAGACCGACTCGGCGTCCAGGACCGGCGCGCCGGTCTCGTCGGTCAGCGAGATCCGCCGCCCGCCTTCCAGCGGGGATACGCGCACCCGTACGCGGCGCGCCCCCGTGGCCCACAGCGACACGCCGCTCCAGGCGAACGGCAGCCACACCTGACCGTCCCGCGACGCGCCCTGACGGCCGCCGTCCAGGAGCGTCGGGTGCAGGGCCGCGTCCAGCAGCGCCGGGTGGATGCCGAACCGGTCGGGGCCTTCGCCGGCCGCCTCGGGCAGCGCGATCTCGGCGAGCAGATCGGCGCCGTCCCGCCACGCCGCGGTCAGACCGCGGAACGCGGGGCCGTAGCCGTAGCCGCCGTCCGCGGCCCGTTCGTAGAAGTCCGCGACGTCCAGCGGCTCCGCCGCCCGCGGTGGCCAGGGCGCCGCCGCGGTGTCCGGCGGGGCGGCCGGGTCCGCGGCGTCCTCGGGCGCGAGCGTGCCCATGGCGTGGCACACCCACCCGTCGGGCGCCTCCGTGTCCAGCTCGTCGGGGCGCGAGTGCACATCCACGGTCCGCCTGCCGTCGTCGTCGGCGGGCCCCACGGCGACACGAATGCGCAGGGCCCCCGAGGCGGGCAGCACGAGCGGGATCCGCAGGGCCAGCTCCTCCACCGCGGCGCAGCCCGCCTCGTGCGCGGCCTGCAGCGCCCACTCGACCAGGGCCGCCCCGGGCAGCAGCGCGGTGCCGCCGACGACATGGTCGGCGAGCCAGCCGTCGCCCGTGGCCGGAAGGCGCCCGGTCAGCACCATGCCGCCGTCGGGCAGGCTCACCGCGGCGGCCAGCAGCGGATGTTCCACCGCGCGCAGACCGGCGGCGGACATGTCGCCGGTGCCCTTTCCGCCGAACGGTATCCAGTAACGGCGGCGCTGGAAGGGATAGGTGGGCAGCTCGACCGTGCGGGGGACGCCGTCGCCCGCGAACCAGGGGCGCCAGTCGACCCCGAGACCCGCGGCGTGCGCCTCGGCGAGCGCGAGGGCCAGCTGGTGCGCGCCGCCGTGGTCGCGGCGGAGGGTGGCGAGGGAGGTGGCGGGGACGCCGGACTCCTCGAAGGTCTCCTGCAGGCTCGTGGACAGGATGGGGTGCGGATTGCACTGGATGAACACCCGGTATCCGTCGGCCAGCAGCCGGTCCACGGCCTCGGCGAAACGCACCGGCCGGCGGAGGTTGTCGAACCAGTACTCGGTGTCGAGCCCCGCGGTGTTCAGGCGTTCCGCGGTCATGGTGGAGTAGAACGCGATCTCGGACTCGACCGGGGACACCTCACCCAGCGCCGAGACGACGTCGTCGTGGATGCGGTCCACGTGCGGGCCGTGGGAGGCGTACGCCACGTCGATGGTCCGAGTGCGCGCGCCGCGCTCCTCCGCCGCCGCCAGCACGGCGGCCAGAGGCCGCGGGGAGCCGGAGACGACCGTGGCCCGCGGGCCGTTGACGGCGGCGACGGCCACGTCCCCGGCCGCCGGGCCGAGGTCGCCGATGAGCCGGGCGGTGTCCTCGGCGCTCAGCGTGAGGGAGGCCATGGCGCCGTGGCCGATCAGCGGGCGCAGGGCCTGGACCCGCAGGGCGATGATCCTGGCGGCGTCGTCCAGGGTGAGGGCCCCGGCGACACACGCGGCGGCGACCTCGCCCTGGGAGTGCCCGACGACGGCCGCCGGGGTGATGCCGAAGCTCTGCCACAGCGTGGCCAGCGAGACGTGTACGGCCCACAGCACGGGCTGGACGACATCGTCCCGCTTGGGGTCCGTCGCACCGTCCGTGCCGCGCAGCACGGCGGTCAGCGACCAGTCCACGTACGGCGCCAGGGCCCGCTCGCACTCGGCGATCCGGGCGGCGAAGACCGGGGAGCAGTCGAGGAGTTCACGCGCCATGCCCGGCCACTGCGCCCCCTGGCCGGGGAAGACGAACACCGGGCCGACGCCCGTCGGGCCCGCCGCGCCGGTGACCACCGCGGGATCGGGCTCGTCGGCGGCCAGCTTGGCCAGACCGCCCAGCAGCGCCGACCGGTCCGTGCCGACCACCACGGCCCGGTGCTCGAACCGGGCCCGCGCCGTCGCCAGCGACCAGCCCACGTCCGCCACCTCGGCGGCCGGATCGCGCTCCACCGCCTCGGCCAGCCGCCGCGCCTGCTCCGCCAGCGCCGCCTCGCCCCGCGCGGACAGCACCCACGGCACCACGGTGCCTCTTATTGCGGCTCGGTTCGCCTCCGGGGCGCCCTCCTGGCTCTCCGGGGCGGGGCCGGCCGGGGTGGTTTCGGCCGCGTCGGGTGCCTGCTCGATGATCACATGTGCGTTGGTCCCGGACACGCCGAACGAGGACACCCCGGCCCGGCGCGGGTGGTCCGTCTCCGGCCAGTCGACCGGCTCGGTCACCAGGCGGATGGCCCCCGAGCTCCAGTCCACATGTGGCGTCGGCTCGTCGATGTGCAGCGTCGCGGGGATCGTCCCGTGGCGCATCGCCATCACCGTCTTGATGACGCCCGCCGCCCCCGCCGCGGCCTGGGTGTGGCCGATGTTCGACTTGAGCGAGCCCAGCAGCAGCGGCCGGTCGGCCGGCCGCTGCCGCCCGTACGTCGCGTGCAGCGCGTCCGCCTCGATCGGGTCGCCGAGCCGGGTGCCCGTCCCGTGTGCCTCCACCACGTCCACCTCGGCGGACGACAGGCGGGCGTTGACCAGCGCCTGGCGGATGACGCGCTGCTGGGAAGGGCCGTTGGGCGCGGTGATGCCATTGCTCGCGCCGTCCTGGTTGACGGCCGAGCCCCGGATGACGGCGAGCACCTGATGGCCGTTGCGGCGGGCGTCCGACAGCCGCTCCAGCAGGATCAGACCGGCGCCCTCGCCCCAGCCCGTGCCGTCGGCGGCCGCGGCGAACGGCTTGCAGCGTCCGTTCGGCGCCAGACCGCGCTGGCGGGCGAAGCCGGTGAACACCGCGGGCGAGGCCATGATGGTCACGCCACCGGCCACCGCGAGTTCGCACTCGCCCTGCCGCAGCGCCTGAATGCCCAGATGCATCGCCACCAGCGACGCGGAGCAGGCCGTCTCCACCGTCAGCGCGGGGCCCTCGAGGCCGAAGGTGTACGACAGCCGGCCCGACAGCATGCTGGGGGCGACGCCCGCGTACCCCTCGGTCTCCTTGCGGACGGGGACACCCGATGTGGCCGAGCCGATGGT GGCGGCCCCGACGAAGACACCGGTCCGGCTGGACCGCAGGCCCGTGGCGTCGATACCGGCCCGCTCGAACAGCTCCCAGGCCACCTCCAGCAGTACGCGCTGCTGTGGGTCGGTGGCCAGCGCCTCGCGGGGGCTGATCTCGAAGAAGCCCGCGTCGAACTTGTCGGCGTCGCGCAGAAACCCGCCCTCGCGCGTATACGTGGTGCCCGGGTGGTCGGGGTCGGGGTGGTACAGGCCGTCCAGGTCCCAGCCGCGGTCGGCGGGGAACGGCCCGACGGCGTCCACACCGGAGGCCACCAGGTCCCACAACGCCTCGGGCGAATCCACCCCGCCGGGGAACCGGCAGGTCATCGCGGTGATGGCAATGGGCTCCTGGGCCCGCTCCTCCACCTCGCGCAGGCGCCGCCGCGTGTCGTGCAGATCGGTCGCCACACGCTTGAGGTAGTCGACAAGCCTCTCTTCATCCGGCATCCGCAGTCACGACCTTCATACGCGTCGAAGCGAGCAACTGAGCCTTCACGAGGTGCCGAGTTCGTTGTCGATGAACGCGAGCACTTCGTCGGCGGTGGCGAGCGTGAGCCGGTCGGTCGCGTTGTCCTCGGCCGGCGGCGCGGACGCCGCCTTCCATTGGGCCAGCAGACCCTCAAGCCGATCTGTCACCGCCCGCGCGTCCGCCTCGGGCAGGTCGAGGGCCGACAGTGTGGACTCCAGCCGCGCCAGCTCGCCGAGCACCGGGTCCACCCCGCCCTGGGCGCCGGCTCCCCCGTCCGCCACCAGCTGGCCGCGCAGATGGTCGGCCAGCGCCCGGGCCGAGGGGAAGTCGAAGACCAGCGCGGCGGGCAGCCGCAGCCCGGTCGCCGCCGAGAGCCGGTTGCGCAGCTCGACACCGGTCAGCGAGTCGAAGCCCAGATCGGTGAACGGCGCCTCCTCCCGTACGCTCTCGGCGTCGGCGTGGCCGAGCACCGTCGCGGCGTTCGTGCGCACCAGTGTGAGCAGCTGCTGCCGCTGCTCGGCCTCCGGGAGTCCGGCGAGCCGGCGCGTCCAGTCGTCGGGGCGCTGCGCGGCCGCGGCGGCGCGCCGCGTGGCCCCGCTCGGGCGGGCGGCCAGGGCGCGCAGCGCGGCCGGGAGGGTGGCCGCCGGCTGTGCGGCCAGCGCGTGGACGTCGAGGTCGAGCGCCACGAGCTGCGGGTCCCCGTGCCGGTGGGCCGCGTCGAAGAGGGCCAGCGCGTGGTCGGTGGCCAGCGCCGTGACGCCGGTGCGGCCCATCCGCGCCACATCGGCCTCGGTGAGCGCTCCCGTGATGTCGCTGGTGGCGTCCCACAGCCCCCAGGCGATGGAGGTGGCGGGCAGTCCCGCGTGGTGTCGCCGGGAGGCCAGGGCGTCGCAGTAGGCGTTGGCCGCGGCGTAGTTGGCCTGTCCTGGGTTGCCCAGGGTGCCGGAGACGGAGGAGTAGAGGGCGAACATGGCCAGCGGGAGGTGCCGGGTGGCCCGGTGGAGGTTGGCCGCGCCCGCCGCCTTGGCCGCCCAGACGCGGCGGAGGGTGTCGGGGGTCTGGGAGGTGACCATCGCGTCGTCGACGACACCGGCGGTGTGGATGACGGCGGTGAGCGGGTGGTCGGGGTCGATCGCGTCGACGGCCTGCCGCACGGCCCGCAGGTCCGAAGTGTCGGCGGCGGTGATGCGGACGGTGGCGCCGAGCTCGGTGAGCCGGGCGGTCAGGTCGGCTGCGCCGGGGGCGTCCGGGCCGCGGCGGCTGAGGAGGTGGAGGTGGGTGATGTGGTGGGTGGTGACGAGGTGTTCGGCGACCAGGGCGCCGAGGGTGCCGGTGCCGCCGGTGATCAGGGCCGTGCCGTCGGGGTCGAGAGCAGGGGGGACGGTCAGGACGATCTTGCCGGTGTGTTTGGCCTGGCTCATGTGGCGCAGCGCCTGGCGGGCCCTGGCCAGCGGCCACGCCTGCACCGGGGGCGGCGTCAGAGCGCCCTCGTCGAACAGCTCCACCAGGGTCGCCAGCATCAGCGCGACGCGTTCCGCCCCGGCGTCCGAGACCAGGTCGTACACCCGGTAGTCCACCCCGGCGAAGTCCTCGGCCACCTGCTCCGGGTCGCGGAGGTCGGTCTTGCCCATCTCCAGGAACCGGCCGCCCTTGGCCAGCAGACGCAGCGAGGCGTCGATGAAGTCGCCCGCCAGGCTGTTGAGGACCACATCGACGCCGCGGCCGCCGGTGGCGGCGCGGAAGGCGGTCTCGAAGTCGAGGTCGCGGGAGGAGGCGCGGTGGGTCTCGTCGATGCCCATGGCCTCCAGCGCGGCGTGTTTGCCGGGGCTAGCGGTGCCGTACACCTCGGCGCCCAGGTGGCGGGCGATCTGCACGGCGGCCATGCCCACACCGCCGGCCGCCGCGTGGATGAGCACCGACTCGCCCGCCTTCAGGCGGCCGAGCTCCACCAGCCCGTACCACGCGGTCAGGAACGCCACGGGGACGGCCGCCGCCCGGGGGAAGTCCCAGGTTGCCGGCATCCTGGTCACCATCCGCGCATCGGCCACGGTGACCTGGCCGAACGCCCCGTCGAAGACGCCCAGGACGCGGTCGCCGGGCGCCACCGTGGTGACGTCGGGGGCCACTTCGAGGACGACGCCCGCGCCTTCGCCGCCGATGCGGGTCTGGTCGGGCACCATGCCCAGGCTGATCAGGACATCGCGGAAGTTGACACCGGCCGCGCGCACCTCGATGCGTACGCGCCCGGCCGCCAGCTCCTCCTCGGCCTCGGGGAACGGCACCGGCGCCACGGAGTCGATCGTCGCGGAGCCCGTCGTGCCCGCGGCGTCCAGCCGCCACGCCCCCTGGCCGACGGGGGGTACGAGCGCGGCCGAGGCGGTGGCGCGGACGAGCCGGGGCACCAGGACCCGGCCGCCGCGCAGCGCCAGCTGCGGTTCGTCGAGGTCGAGCGCGCAGCGGACCGCGTCGGCCACCCGGCCGTCGGACGCCGGAGCGGTGTCCTTGTCCGTGGGCAGGTCGGTGTCCAGCAGGACGAAGCGGCCGGGGTTCTCCGACTGGGCGCTGCGGACCAGGCCCCAGACGGCCGCCGCGACGGGGTCGAGGTCCGTCTCCTCGTCCACGGCCACGGCCTTGCGGGTCAGCACCACCAGCCGGGCGTCGTCGGGCCGTGGTTCGGCCAGCCAGTTCCGCACCAGCGCCAACGCCCGCTCGACGGCCTCCAGCTCGCCGCCGTCAGCGTCTGCGACCGGCGCCTCGGCGAAGACCACCTCGGGCGGTGCGGGCTCCTCCGCCCGTTCGGCGAGGGGAGCCGCGGGCGTCCACTCCAGCGTGAACAGGCCCTCGGTGGTGGCCGGTTGGGGCGCGTCCAGGCGCATGGGCCGGGTGGTGACGGCGTCGGCGGTGAGGACGAGCTGCCCGGTGGCGTCGGTGAGGGTGACGCTGTGCTCGCTCTCGTTGCGGGAGGTGATCCTGACCCGGGCGTGGGTGGCGCCGGTGGCGTGGAGGGTGACGCCGCTCCAGGAGAACGGCAG CCAGACCTCGCCGCCCGAGGTGTCCGTGACGGCGACCAGCGGGTGGAGCGCGCTGTCGAGGAACGCGGGATGGACGGTGAGGGCCTCGGCCGTGTCGTCGGTGGCCTCGCCCGTAGACAGGGTGACCTCGGCGAAGAGGTCGTCGCCGCGCCGCCACAGCGCCTGGAGCCCCTGGAAAGCGGGCCCGTACGCATAGCCGTTCGCCGCCGCGCGGTCGTAGAAGCCCGTGATGTCGACGGGTTCGGCCCCGGCGGGCGGCCATGCCGCCTCCCGCACCTGCGCCGGATGGCCGGTGGCGGGCTCGGCGGCGAGGGTGGCGGTGGCGTGACGGGTCCACTCGGCCGCGGGGGCCGACTGGGGGCGGGTGTAGATGTGCAGGACCCGGCGGTCGGCGTCATCGGCGGTGCCGACGACGATCTGCAGATCGAGGGGGGAGGTCGGGTGGAGGGTGAGCGGGTGGTGGAGGAGAAGTTCCTCGACGTGGGGGCTGTCGGTGTGCTCGGCGGAGCACAGCGCGAGTTCGAGGAGCGCGGTGCCGGGCAGCAGGACGGTCTCGGCGATGGTGTGCTCGGTGAGCCAGCTGTCGTGGTCGGCCGGTGGGACGTGCCCGGTGAGCACCACGCCGCCGTCCGCCAGGGGCGCCGCCGCGTCCAGCAGCGGATGGTCAAGGCCGGTGCCGCCGGGGGCGGCGTTGCCGGAGGTGTGGGGGGCGATCCAGTACGGCCGGCGCTGGAAGCGGTAGGTGGGCAGCGCGACCGTCTCGGGGGCCGGGTCGGCGGGATACCAGGGCGTCCAGTCCAGGGGGGCGTGGGCGATGTGCAGCGACGCCAGGCTGTGGGCCAGGGCCCGCACATCGGGCTGCTTGCCGGTGAGGGTGGCCAGCGCGGTGGCGTTGGTCTCCTCGGGCAGGGTGTGGTGGACGGCGTGGGTCAGCACGGGAGCCGGGCCCAGCTCGAGGAAGACGGCGGTGTCCTCGGCGAGGTGGCTGACGCTCTGGTGGAAGTGGACCGGCCGGCGGATGTGCTGGGCCCAGTACTCGGGGGTGGTGATCTCCTCGCCCGCGGGCCGGCCGGTGAGGTTGCTGATCAGCGGGGTACGGGGCGGGTGGAAGGTCAGATGGCCGATGGCCTCGGTGAAGGGGGCGAGGATGGGCTCCATCAGGGGTGAGTGGAAGGCGTGGGAGACCGGGAGGGAGCGGGTCCTGCGGCCTTGTTCCCGCCACTTCGCGGTGGCGGCGGCCACCTGGTCGGCGGGGCCCGAGATGACCGTGTGGCCCGGGGTGTTCATCGCGGCGATGGCCAGGCCGGGGTGGTCGTCCAGGGTGGCGGTGATCTCCTCTTCGCTCGCGTGGACGGTGGCCATGGCGCCGCCGGGCGGGAGACCGCCCATCAGGGTGGCGCGGACGGCGACCAGGTGGGCGGCGTCCTCCAGGGAGAGCACGCCCGCGAGGTGGGCGGCGGAGATCTCGCCGATGGAGTGGCCCGCGACGGCGTCGGGGGCCAGGCCCATGCTCGTCAGGAGGCGGGCGAGGGCGAGGTGGAGGGCGAAGAGACCGGCCTGGGTGTAGGTGGTGTGGTGGATCAGGTCGCCGTGTCCGGGGTCGGTGGCGAAGACCACCTCGCGCAGCGGATGGTCGAGGTGGGGGTCCAGGAGCGCGCAGACCTCGTCGAAGGCGTCGGCGAAGACCGGGAAGCGCCGGTAGAGGTCGGCGCCCATGCCCGGCCGCTGGCTGCCCTGCCCGGAGAACAGCCACACCACGTTCCCCGGCGCCGTTGACCCGGCGCCGGTGGGGGTGGACAGGCCGGGGTGCGGATCGCCGGCGGCCAGCGCCTCCAGCGCGGCGGTCATTTCCTCCAGGCCTTCGCCGACGACCACGGCCCGCCGTTCGAACACCGCCCGCGTCGTGGCCAGGGACCACCCCACGGCCGCCACCGACGCGCTGCCGTCGGCCCGCAGCTGCTCGGCGAGCGCGGCGGCCTGCTCGCGCAGGGCCTGGTCGTCGCGCGCCGACAGTGGCCAGGGGACGGTGCCGGGCGGGGGAGTGCCGGCCACCGGCCGGGGTTCTGGCCGGTGGGGGGCTTGTTCGAGGATGAGGTGGGCGTTGGTGCCGGAGGCGCCGAAGGAGGAGACGGCGGCGCGGCGGGGGCGGCCGGTCTCGGGCCAGTCGGTGGCCTCGGTGAGGAGCTGGACGTTTCCGCTGGTCCAGTCGATGTGCGGGCTGGGTTCGTCGATGTGGAGGCTGGCGGGCAGCCGTCCGTGGCGCATCGCCATGATCATCTTGATGACGCCGACGATGCCGCTGGCGGCCTGGGTGTGGCCGATGTTGGACTTGACCGAGCCGAGCCACAGGGGCCGGTCCTCGGGGCGGTCCTGGCCGTAGGTGGCCAGGAGGGCCTGGGCCTCGATGGGGTCGCCCAGCGTCGTACCCGTGCCATGGGCCTCCACGGCGTCGATGTCGGCGGGGCCCAGGCCGGCGTTGGCGAGGGCCTGGCCGATGACGCGTTGCTGGGAGGGGCCGTTGGGGGCCGTCAGCCCGTTGGACGCGCCGTCCTGGTTGATCGCCGAGCCGCGCACCACCGCGAGCACCTGGTGGCCGTTGCGGCGGGCGTCGGAGAGGCGTTCGAGCAGGACGAGACCGGCTCCTTCGCTCAGGCTGGTGCCGTCGGCGGCGGCGGCGAACGGCTTGCACCGCCCGTCGGGGGCCAGCCCGCGCTGCCGGGAGAACCCGGTGAACGGGTCAGGGGTGGCCATGACCGTCACCCCGCCCGCGAGCGCCAGATCGCATTCGCCCTGACGGAGGGCCTGGGCGGCGAGGTGCATGGCGACCAGGGAGGAGGAACAGGCGGTGTCCACGGTGACGGCCGGGCCCTGGAGGCCGAGGGTGTACGCCACCCGGCCGGAGATGATGCTGCCGATGCTGGACAGCATGGTGTAGCCGTCCAGCCGGGGGTCGCCCGCGGGCAGACCGGCGGCGTAGTCGTGGTAGATGACACCGGTGTAGACCCCGGTCAGGCTCTCCTTGAGGGTGTGCGGGTCGATACCGGCGCGTTCCAGGGCCTCCCAGGCGGTTTCCAGGATCAGACGCTGCTGGGGGTCGATGGCCAGCGCCTCGCGGGGGTTGATGCCGAAGAACGCGGCGTCGAAGCCGTCGGCGTCGTAGACGAACGCGCCCTGGGAGGCGTAGGTCGTGCCCGGGTGTTCCGGGTCGGGGTGGAACAGGTTGGCCAGGTCCCAGCCGCGGTTGGTGGGGAAGGGCCCCAGGATGTGCTCTCCCGCCGCGACCAGGTCCCACAGCCCCTCGGGCGTGGTCACATCGCCCGGGAAGCGGCACGCCATCGCCACCACGGCGATGGGCTCGTCGTCCGCGCTCGCGGCCCGGGCCGGCCCCACCGGCACCGCCGTGGCGGGCGCGGTGCTGGTCAGGTGCTGCAGGAGGTGGTCGGCCAGGGCCCGGGGGGTCGGATAGTCGAAGACGAGGGTCGAGGGCAGCCGCAGCCCGGTGGCGTCGACCAGGCGGTTGC GCAGCCCGACGGCCGTGAGGGAGTCGAAGCCCAGCTCCTTGAAGGCGGCCTCGGCCCGGATCGCCGCGTCGGAGGAGTGGCCCAGCACCACGGCGGCCATCCGGCGCACCACATCGAGCAGCAGTTCGCGCTGTCCGGAGGCGTCGAGACCGTCGAGGCGCGAGGCCAGGGCGAGGACCGCCGACTGCCGTTCCGCGGCGCGGCGCCGGCCGGGCCGGACCAGGGCGCGCAGCAGCGCGGGAGCCGCGGGCCCCAGCCGGGCGACGTCCAGGTCCGCCGCCACGACCAGGGGGTGTACGGCGTGCAACGCCGCGTCGAGCAGGTGCGAGGCGTCGGGAGTGGCCTCTTCCCCCAGGGACCAGGCCAGGCCCGTACCCGGGAGACCCGCTGCGCGCCGGTGGGCGATCAGCGCGTCGGTGTGGGCGTGAGCCGCGGCGTGTCCGGCCCGGCCGGGGCCGCCCGTGACATCGGCGGCGGAGGAGAACACCACGAACATCCGCAGCGGCAGGCCATGGGTGAGGGCGTGCAGGTTCCACACGCCGGTGGCCCCCGGCGCCCACGCCCGCGACACTCGCCCGTCGTCCCAGGACTCCACCGGGGCGCCGTCGGACGCCCCGGCCAGATGCACCACGCCCACCAGCGGGTGGCTGTCGGGCACGGAGGCCAGAACCTCCGCCAGAGCGGAGCGGTCGGCGACATCGACCGCCGCCACCTCCGCCCGGGCGCCCAGCGCCGTCAGCCGCTCGGCCAGGTCGGCCGCGCCGGGGGCCCCGCTGTCCGGGTGGGAGAGGAGCAGCAGACGCTCGGCGCCGTACGCGCGCACCAGTTGCTCGGCGACCTGGCCGCCGGCCGGGTGGTCCGCCCCGCCGGACACCACCACCGTGCCGCCGGACAGTTCGACCGCCGTGTCCTCATCGGGGCGGGTGACGGCGCGTACCAGGCGGGGGACCGACACCGCGCCCGAACGGACTGCGATCTGCGGCTCGTCCGCGGCGACGGCCCGGCGCACCGCCTCGACCAACGTCTCTTCCGCCCCCGAGCCCGAGCCCGTGCCCGAGTCCGTGCCCGAGTCCGAGCCCGCGCCCGCTTCGGCGCCCAGGTCGACCAGCGTGAAGCGGTCCGGGTGCTCGGCCTGGACCGCCCGGACGAGGCCGAGCACGGCCGCGCTCCCCGGATCCACCGGCCCGTCGTCCTCGCCGGCCGAGGCCGCGCCGGAGGTGATCAGCACCAGCCGGGAGTCCCGCAACCTCGGCTCCGCCAGCCAGCCCTGGACCAGCTCCAACGTGTGCCGGACGGTGACCGGAGCCGTGTCGGCCCCGGAGCCGGCCGCAGGTGGCACGGCGGCGAGGACCACGGGCGGAGCGGGCTCTGCCTCGTCCACCGCCGACAGCAGCGCGGCCAGGTCGGCGTAGTGGCGCCCGGGGCCCAGGTCGCCGAGCGTCGCCCAGCGCTCCTCGGCCTCACCCGGCTCGGGCCGGCTCTCCGGCGGCGGCAGCGGTGTCCACTCCACCGTGAACAGGCCGCCGGCGGAGCGGCGTTCCGCGGCCTTCAGCCAGTCTCCGTCGGCGGGCCGCAGCGCGACCGAATCCACGGTGAGCACCGGGGCGCCGGCCGGGTCCGCGACGACGACGGTCAGCTCACGGCCTTCGGCGTGCTCCCGCTGTCCGATCCGGACCCGTACGCCGGTGGCGCCGCCCGCGTGCAGGGACACGCCGCTCCAGGAGAACGGCAGCCACAGCGGGCCGTCGGTGTCCGCGTCGTCCAGCGCCACCGGGTGCAGGGCGCATTCGAGCAGCGCGGGATGGATGCCGAACTCGTCGCCGCCCTCCTCGGCCCCCTGGTCCAGGGCCACGTCGGCGAGGAGGTCGGCGCCGTGCCGCCACACCGCCCGGACGCCCTGGAACGTCGGCCCGTACCGGTAGCCGGCGGCCTCGGCGCGCGCGTACAGGCCGGTGAGGTCCACCGGTTCGGCACCGGCCGGTGGCCAGGCGCCCGCCAGCTCAGTCCCGGCGGCTGGGGAGACCGTGAGGGTGCCCGTCGCATGGCAGGTCCAGGTGTCCGGCCCGTCGGCTCCGTCCGGTGTGTCGACCGCGGGCTGGGTGGACATGCGTACGGCCCGCCGGCCGTCCTCGTCGGGGGCGTCCACCACGACCTGGACGCGCAGCTCGGAGGCGGCGGACAGGGTCAGGGGGACCTCAAGGGTCAGCTCCTCGACACCGCCGCAGCCCGCCTCGTCGGCCGCCCGCAGCGCCCACTCCACCAGGGCCGTGCTGGGCACCAGGACCGCATCGGCGAACTCGTGCTCGCCCAGCCAGCCGGCGTCCCCGGCCGCCGGCAGCCGCCCGGTGAGCAGGAGCCCGCCGTCGGCGAGGCCCGTCGCGGCCGACAGCTGCGGGTGGTCCACCGGCCGCAGCCCGGCCGCGCTGACGTCACCGGCCCGGCGGCCCGCCGCCGACCAGTAGCGCTGCCGCTGGAAGGGGTAGGTGGGCAGATCGGTGGTGGCGGGCGGGGTGGCGGCGTACCAGGGCCGCCAGTCGACGGCGAGGCCGGTGGTGTGGGCGAGCGCGAGGGCGTCCGCGAGGTCGTGGGGGGTGCCGTGGTCGCGGCGGAGGGTGGGGATCGTGGTGGCGGGGAGGTCGGCATCGTCGATGGCGTCCTGGATGCCGGGGGTGAGGACGGGGTGGGGGCTCGCCTCGATGAAGTGGCGGTGGTGGTGGTCCAGGAGGGCGGTGAGGGTGTCGGTGAAGCGGACCGGGCGGCGGAGGTTGGTGAACCAGTACTCGGCGTCGAGCGGGGTGCCGGTCAGCTGCTCGCCGGTGACGGTGGAGTAGAAGGGGATGGTGGGCGTGGTGGGGGCCAGGTCGGCGAGTTGCGCGAGGATGGTGTCGCGGATGCGGTCGACGTGCGGGCTGTGGGAGGCGTAGTCCACGTCGATGGTGCGGGTCCTGGCGCCCTGTGCCTCGGCGGCCGCCAGGACCGTGGCGATCTGGTCCGGCGGTCCGGAGACGACGGTGGAGCGGGGGCCGTTGTGGGCGGCGACCGCGACGTCCTCGGCCGCCTGGCCGAGTCCGGCCAGGACGCGGGCGGTGTCCTCGGCGCCGAGGGTGAGGGAGGCCATGGCCCCGTGGCCGGCGAGGGCGCGCAGCGCCCGGCTGCGCAGGGCCACCACCTTGGCGGCGTCGTCGAGGGTGAGGGCCCCGGCCACACAGGCGGCGGCGATCTCGCCCTGGGAGTGGCCGATCACGGCGGCGGGGGTGACGCCGAAGTCCTGCCACAGGCCCGCCAGGGACACCATGACGGCCCACAGGGTCGGCTGGATGACATCCACGCGGTGCGGGTCGGTGGTGGTGTCGGTGGAGCGCAGCACGGCGGTGAGCGACCAGTCGACGTAGGGCGCCAGGGCCCGTTCGCAGGCGGCGATCCGGGTGGCGAAGGCGGGCGAGGAGTCCAGCAGTCCGACGGCCATGCCGGGCCACTGGGAGCCCTGGCCGGGGAAGACGAAGACCGGGCCC GCCTGTGGGTCGAGGGCGGTGCCGCGGACGAGGCGGGGGTGTTCGCCGCCGGTGGCCAGGGCATCGAGCGCGGCGGTCAGGTCGGCGAGTTCGGTGCCGGTGACCACCGCGCGGTGGTCGAACGTGGCGCGGGTGGTGGCCAGCGACCAGGCCACCTCGGCGGGCGCGGCGCCCGGGGCGCCGGTGCCGGTGACGGCCCGGGCCAGCCCCCGCGCCTGGTCGGCCAGGGCCTGTGGGGTACGGGCCGAGAGCAGCCACGGCGCCACGCCGCCCAGGACGGCAGGCTCGGCGGCCTCCTCCTCCGCTGTCTCCGCCTCGGCCTCCGGCGCGGCGCCCTGTGCCTCCACCGCCGTGGGCCGGGCCGCCTCCTCCAGGATGACGTGGGCGTTGGTGCCGGAGATGCCGAACGCCGAGACGCCGGCCCGGCGCGGGCGGTCGGGGGCCGCCGGCCAGTCGACCGGCTCGGTCAGCAGCCGCACCGTGCCGCGGCTCCAGTCCACATGCGAGGTGGGCTCGTCGACGTGGAGGGTGGCGGGCAGCGAGCCGTGGCGCAGGGCCATCACCATCTTGATGACGCCCGCGATCCCGGCGGCGCCCTGCGTATGGCCGATGTTGGACTTGATCGACCCCAGCCACAGCGGGCGGTCCTCATCCCGCTCCTGGCCGTAGGTGGCCAGCAGCGCCTCGGCCTCGATCGGGTCGCCGAGCGTGGTGCCCGTGCCATGGGCCTCCACCGCGTCCACCTCGACCGTGGCCAGCCCGGCGTTGGCCAGCGCCTGGCGGATCACCCGCTGCTGCGAGGGCCCGTTGGGGGCGCTCAGCCCGTTGCTCGCGCCGTCCTGGTTGACGGCCGTACCCCGGATGACGGCGAGCACCTGGTGCCCGTTGCGCTCGGCGTCCGACAGCCGCTCCAGCAGCACCAGGCCCGCGCCCTCGGAGAAGCCCGTGCCGTCGGCCGCCGTGGCGAACGGCTTGCACCGGCCGTCCGGGGCAAGGCCCCGCTGCCGGGAGAACTCCGTGATCACGTTCGGGGTCGCCATGACCGTCACCCCGCCGGCCAGGGCCAGATCGCATTCGCCCTGCCGCAGCGCCTGGACCGCCAGGTGCATCGCCACCAACGAGGACGAGCACGCCGTGTCCAGCGTGACCGCGGGCCCCTCCAGGCCGAGGGTGAAGGCGATACGGCCGGACACGACGCTGAGCGCGTTGCCCGTCAGCAGGAACCCCTCGGCGCTGCGCTCGATGTGCGGTGTGCCGAAGCCGGGCACACCACAGCCCGTGTAGACGCCGGTGGGGGTGCCTTTGAGGGCCGTGGGGTCGAGGTGGGCGCGTTCGAGGAGTTCCCAGGCGGTTTCGAGGAGGAGCCGCTGCTGCGGGTCCATGGCGAGGGCTTCGCGCGGGGAGATGTTGAAGAAGGAGGCGTCGAATTCGTCGGCGTCGTGGACGAAGCCGCCTTCCCGGACATAGCTGGTGCCGGGGTGGTCGGGGTCGGGGTGGTAGAGGGCGGCCAGGTTCCAGCCGCGGTTGGCCGGGAAGGGGCCGATGGCGTCGGTCCGGCTGTCGACCAGCTCCCACAGGGCTTCCGGGGAGGTGACGCCGCCGGGGTAGCGGCAGGCCATGGAGGTGATGGCGATGGGCTCGTGGTGGCGTTGCTCCGCTTTGCGCAGGCGCTGCCGGGCCTCGCCCAGGTCGACGGTGACCCGCTTGAGGTACTGGCGCAGCTTGTCTTCCGTGGACGGCATTGGTCCTCCCCCACCTCTCGTCAGGACGAGCCCAGTTGCTGGTCGATGAGCGCGAACATCTCGTCGTCCGAGGCGGACTCCAGCGCCTCGCCGTCGATGTCCGCCGCACTGGCCGTGGACCCCTCCAGCCTGGACAGCAGGGCGTGGAGCCGGCCCGCGATCGTCTCGCGCGCCTTCCCGTCCACGTCGTGGCTGCCCAAAGTGGACTCCAGCCGGGCCAAGTCGTTCAGGACGGGCGCGAGTCGGTCGACGGCCGCATCCGTCCGGTCCGCGGGGGCGAGCTTCTGGTCCAGGAAGCGGGCCATGGTCTCCGCGTCGGGATAGTCGAAGACGAGCGCGGCGGGCAGCCGCAGCCCGGTCGCGGCCGAGAGCCGGTTGCGCAGCTCCACCGCGGTCAGCGAGTCGAACCCCAGCTCCTTGAAGGAGGTGTCCGCGCGGAGCGCCGCGGGGTCGCTGTGGCCGAGCACCGTCGCCGCGTGCGTCCGCACCAGGTTGACCAGCTCGCGGTGGCGCTCGGCGTCGGAGAGCGCGGCCAGGCGGCCGGCCCAGTCGGTGGACCCTGCGACGGCTGCGGTACGCCTGACCGGTGCTCCCGCGGCGGCGACGGTACGCAGCACGGCGGGCACCGCGTCGGACGGCTGGGCGCCGAGCGTCCGCGGATCGAGGTCGAGCGCCAGCGGGGTCGCGCCGCCCTGGCGCCGGGCGGCGTCGAACAGCGCCAGCGCCTTGGGGGTGGACAGCGGGGTGAAGCCGGTGCGGCTCATCCGGGCCAGGTCGCCCTCCGCCAGATGGCCGGTCATCTCGCTGGTGGCGGCCCACAGTCCCCAGGCGATGGAGGTGGCGGGCAGTCCCGCGCGGTGTCGCCGGGTGGCGAGGGCGTCGCAGTAGGCGTTGGCGGCGGCGTAGTTGGCCTGCCCCGGGGTGCCGAGGGTGCCGGCGGCCGAGGAGAAGATCACGAACATGGCCAGGGGGAGCCCCTGGGTGGCCCGGTGGAGATTGGCCGCGGCCGTCGCCTTCGCCGCCCAGACCGACGCCAGCCGCTCCGGGGTCTGCGCGGTCAGCACCGCGTCGTCCAGGGCCCCCGCGGCATGAATGACACCGGTGAGCGGGTGGTCGGGCTCGACGGTGTCGAGGGCCTGGCGCAGGGCCTGGGGGTCGGTGGTGTCGGTGGCGGTGATACGGACGGTGGCGCCCAGCTCGGTGAGCCGGGCCGTCAGATCGGCGGCGCCGGGAGCGTCCGGGCCCCGGCGGCTGAGCAGGTGGAGGTGGGTGATGTGGTGGGTGGTGACGAGGTGTTCGGCGACCAGGGCGCCGAGGGTGCCGGTGCCGCCGGTGATCAGGACGGTGCCGTCGGGGTCGAGCGTGCGGGGAATGTCCAGCACGTATTTGCCGGTGTGCTTGGCCTGGCTCATGTGGCGCAGCGCCTGGCGGGCCTCGGCCAGCGGCCACGCCCGCACCGGCGGCGGCGCCAGGACGCCCCGCGCGAAGTGGCCGGCGAGCGTGTCCAGCATCTCCGCGACCCGGTCCAAACCGGCGTCCGGCACCAGGTCGTAGAAGCGGTAGCGGACACCGGGGTACGCCTCGGCGATCTGCTCGGCGTCGCGCTTGTCGGTCTTGCCCATTTCGACGAAGCGCCCACCTTCGGACAGCAGGCGCAAGGACGCGTCGGTGTACTCGCCGGCGAGGCTGTTGAGGACCACGTCGACGCCGCGGCCGCCGGTGGCGGTGCGGAAGGCTTCCTCGAAGTCGAGGTCGCGGGAGGAGGCGCGGTGGGCCTCGTCG ATGCCCATGGCCTCCAGTACGGCGTGTTTGCCGGGGCTGGCCGTCGCGTACACCTCGGCGCCCAGGTGGCGGGCGATCTGCACGGCCGCCGTGCCCACGCCGCCCGTGGCGGCATGGATGAGCACCGACTCGCCCGCCCTGAGGCCCGCGAGGTCCACCAGCCCGTACCAGGCGGTCAGGAACGTCGTCGGGACGGCCGCGGCCTGCTGGTCCGTCCAGCCCGGCGGGATGGGCACGACCGCGCGCGCGTCGGCGACGGCCACGGGCCCGAAGGCGCCCTCGAACAGCCCGAAGACGTGGTCGCCGACGGCCACCGACGTCACGTCCCCGGCCACCTCCAGCACCACACCCGCGCCCTCGCTGCCCCGGAACACCCCCTCGTCCGGATACATCCCGAGCACGATCAGCACATCGCGGAAGTTGATGCCCGCGGCCCGCACCCCGATCCGCACCTGGCCGGGCGCCAGCGGCTCCAGCACCTCCGGGCAGGCCACGACCGAAACGCTGTCGATGGTGCCGGTGTCACCGGCCGCCAGCCGCCATGCCGGTTCGTCCGGCGGCGGCATCAGCTCCGGTGCGCCCGCCCGCTCCAGCCGGGGCGCCAGCACGCGGCCACCGCGCACCGCCACCTGTGGCTCGTCCAGGGCCACCGCCCGGGTGACGGCGCCGAGCACATCGTCGGGCCCGGCGCCGTCGTCGATGTCGCACAGCAGGAAACGGCCCGGGTTCTCCGCCTGTGCGCTGCGCAGCAGCCCCCACACGGCGGCCGCCGCCGGGTCGGGGCCGTTGTCGGTTTCCGCAGCCGCCACCGCGCCCCGGGTGACCAGCACCAGCCGGGCGTCGGCCGGCTCCGGCGTGTCCAGCCACCGACGCGCCAGCGTCAGCACACCGGTGGTCACGGCCAACGCGCCGTCGGCCGATTCCCGGTCGCCCTCGGCGGCCGGAACGCCGACGAGCACCGTGTGAGGCCTGGCGGGGGCGGCGAGCAGGGCGTCGATGTCGGCGTGACGGGGCGGCGATCCGGCCTCTGACGGGTCGAACGGGAGCGGGCCGCCGTCCAGCAGCGCCCACCCCTCGGCGGCCCCCGCCTCCACCTGCGCCGTGGTGTCCGACGGTGTCGGCAGGGGCACCCACGCCACCGCGTACAGCCCGTCCACCCGCCGCGCGCCCGCCTCGCGCAACTGCCGCGCGCTCGTCGGGCGTACGACCACGGACTCGGCGCTGAGCACCGGCTGGCCCGCCGTATCGGCCACCACCAGCCGGAAGCCGTGCTCCAGCGGGGACATCCGGACCCGGGCCGCGCGTGCCCCGGTGGCCCACAGCGACACACCGCTCCACGCGAACGGCAGCCGCATCCGCCCGTCCTCACCCCGGCCGTCCAGGGCCAGAGGGTGCAGGGCCGCGTCGAGCAGCGCGGGGTGCAGGGAGAACCCGTCGGCCCCCTCGTGGGCCGCCTCCGGCAGCACCACCTCGGCGAGCAGGTCGGCGCCCTGCCGCCAGGCGGCGGTCAGGCCCCGGAAGGCCGGGCCGTAGCCGTAGCCGATCGCCTCGGCGCGGCCGTAGAAGCCGGCCACGTCCAGCGGCTCGGCGTCCCGCGGCGGCCACACCCCCGCCGGCGCCGGGTCCGCGGCGCCCGGGGCGGTCGGGGAGAGGTGTCCGGTCGCGTGGCAGGTCCAGGGCGACGAGCCGTCCTCCGTTTCGGGGCGGGAGTGCACCCGCACCTCGCGGCGGCCGTCCCCGTCGGGCGCGTACGCCACGACCTGGACCCGCAGCCCACCGGACGCGGGCAGCGCCATCGGAACATGGAGCGCCAGCTCCTCGATCCCGCCGCACCCCGCCTCGTCGGCCGCCCGCAGCACCCACTCCACCAGGGCCGTGCCCGGCACCAGGGGGGTGTCGGCGACGACGTGGTCGGCGAGCCAGCCGCCGTTCCCGGCGGCGGGCAGCCGGCCGGTCATCACCAGCCCCCCGTCGGCCAGTTCGACCGCGGCGCCGAGCTGGGCGTGGGCCAGCGAGGTGAGTCCGGCGCCGCGCAGATCGCCCGGTCCGCCGTCCATGCCGAGCCAGTAGCGGCGGCGCTGGAAGGGGTAGGTGGGCAGATCGGTGGTGCGCGGCCCGGCCGGGTACCACGGCGCCCAGTCGATGGCCAGACCGGTGGTGTACGCGCGGGCCACGGCCTGGGCGAGCTGGTGGGGCCCGCCCTGGTCGCGGCGGAGGGTGGGGACGGCGGTGGCGGGGGCGCCGGCCGCCTCGAAGCCGTCCTGCATCCCGGGGATCAGCACGGGATGCGGGCTGACCTCGATGAAGTGGCGGTGTCCATGGGCGAGGAGGGCGGTGAGGGTGTCGGCGAAGCGGACCGGCCGGCGGAGGTTGGTGAACCAGTAGTCGGCGTCGAGCCCTGCGGTGTCCAGGCGTTCGGCGGTCACCGTGGAGTAGAAGGCGATGTCGGAGGCGGTGGGCGCGAGGTCGCCCAGGACGGAGACGATGTCCTCGCGGATGCGGTCGACGTGGGGCCCGTGCGAGGCGTAGTCCACATCGATGGTGCGGGTCCGCGCGCCGCGCGCCTCGGCGGCCGCCAGGACGGTGGCGATCTGCTCCACCGCGCCGGAGACGACGGTGGTGGCGGGCCCGTTGGCGGCGGCCAGGGTGACGTCGTCGGCCTGGTCGCCCAGCTCGTCCAGGAGCTGGGCGGTGTCGTCGGCGCCGAGGGTCAGCGAGGCCATGGTGCCGTGGCCGGCCAGGGCGCGCAGCGCCTGGCTGCGCAGGGCCACCACCTTGGCGGCGTCGTCGAGGGTGAGGGCCCCGGCCACACAGGCGGCGGCGATCTCGCCCTGGGAGTGGCCGATCACGGCGGCCGGGGTGATGCCCTGGTGCTGCCACAGGGCGGCCAGGGACACCATCACCGCCCAGAGGGTGGGCTGCACGACATCGACGCGCTGGAGGTCGGCGGCTTCGGTGCCGGTGCCGGTACCGGTGCCGGTGTCGGCCTCGGTGGAGCGCAGTACGGCGGTGAGCGACCAGTCCACGTACGGCGCCAGGGCCCGCTCGCACTCCGCGATCCGGGCCGCGAACACCGGCGAGGTGTCCAGCAGTTCCACGCCCATGCCGCGCCACTGCGACCCCTGGCCCGGGAAGACGAACACCGGGCCCGCGGCCGCGGCGGTCGCCGCTCCCGCCACCACCGCCGGATCGGGCTCGTCCGCGGCCAGCTTCTCCAGACCGGCCAGCAGCGCGTCCCGGTCCTCGCCCACGACCACCGCCCGGTGCTCGAAGGCCGAGCGGGTGGTCGCCAGCGACCAGCCCACCTCCGCCACCTCGGCCGAGGGGAGCCACCCCGCCAGGTTCGCGGCCTGTGCGCGCAGCGCCTCGGCGGTGCGGGCGGAGAGCACGAAGGGCACGATGCCGCCCGCGGCGGCGGACTCCGGCTGCTCCGGCCGTGGTTGTCCGGGCTGGTCGGGCGCCTGTTCCAGGATGAGATGGGCGTTGGTGCCGGAGATACCGAAGGAGGAGACCGCGGCGCGGCGG GGCCGGCCGGTCTCGGGCCAGTCGGTGGCCTCGGTGAGCAGCCGGACGTTTCCGCCCGCCCAGTCCACGTGCGGGCTGGGCTCGTCGACGTGCAAGGTGGCGGGCAGCCGGCCGTGGCGCAGGGCCATGACCATCTTGATGACGCCCGCCGCACCGGCGGCGGCCTGGGTGTGCCCGATGTTCGATTTCACAGAGCCCAGCCACAGCGGCCGGTCGTCCGGGCGGTGCCGGCCGTAGGCGGCCAGCAGGGCCTGGGCCTCGATGGGGTCGCCCAGCGTCGTACCCGTGCCGTGCGCCTCCACCACATCGACCCCGGCGGGATCCACCCCCGCCGCGGCCAGGGCCTGGCCGATCACCCGCTGCTGGGAGGGGCCGTTGGGCGCGGTCAGGCCGTTGCTCGCGCCGTCCTGGTTGACCGCCGAACCCCGCAGGATCGCGAGCACCCGGTGCCCGTTGCGGCGCGCGTCCGACAGCCGCTCGAGCACCAGCAGCCCGGCGCCCTCGCTCCATCCCGTGCCGTCGGCGGCGGCCGCGAACGGCTTGCACCGCCCGTCCGGCGCCAGGCCGCGCTGGCGGGAGAACTCGACGAACGTGTTCGGCGTGGCCATCACCGTCACACCGCCCGCGAGCGCCAGCTCGCATTCGCCCTGCCGCAGCGCCTGCGCGGCCAGGTGCATCGCCACCAGCGACGACGAACACGCCGTGTCCACGGTCAGCGTCGGCCCCTCCAGGCCGAAGGTGTAGGCCACCCGGCCGGACGCCACGCTCGGCGTGGAACCGGTCATGATCTGGCCCTCGTACCCCTCCGGGATCCGGCCGAGGAACCGCGCGCCGTAGTCGTCGTACATGATGCCGGTGACCACCGCCGTCCGGCTGCCGCGCAGCGCCGCCGGATCGATCCGGGCGTGCTCGAACGCCTGCCACGCCGTCTCCAGCAGCAGCCGCTGCTGCGGGTCGGTGGCCAGCGCCTCCCGGGGGCTGATCCCGAAGAACTCCGCGTCGAACAGGGCCGCCTGGTGCAGGAAGCCGCCCTCCCTGGCGTAGCTCTTGCCGGTGCGCTCGGGGTCGGGGTCGTACAGCCCGGCCACGTCCCAGCCGCGGTCCTCGGGGAACGGCCCGATCGCGTCCACCCCGTCGGCCACCAGCCGCCACAGCTCGTCCGGGGAGTGCACATCGCCCGGGTAGTGGCAGGCCATGCCGACGATCACGACGGGGTCCTCCGCGCTCTCCGACCGGGCCGGCGCTCCGGCCCCCGGCGGCAGCGGCGCGGTGGCCGCGGTGGCGCCGAGCAGTTCGGTGCGCAGATGGCTCACCAGGGTCTCGGGGGTCGGGTAGTCGAAGACGGCCGTGGCCGGGAGGCGGACACCCGTGGCCTCATGGATCGCGTTGCGCAGCTCCAGCGCGGTCAGCGAGTCGAGACCGAGCTGCGAGAACGTCCGGCCCAGTTCGAGGGTTTCCGGGTCGGGGTGCGCCAGGACGGCGGCGATCCGGTCGCGTATCAGCTCCCCGATCACCCGGTCCTGTTCGCTCTCCGGCAGCCCGGCGAGCCGCCCGGCCCAGGAGGGTGACGCCTCGGTAGCCGCGGCCGTACGGACGGGCAGGCGCACCAGGCGGCGCAGCAGCGGGGGCAGCGTGCCCGCGGCCGCCTGCTCGCGCAGGACGGCGAGGTCGAACCTGGCCGGGATCAGCACGGGCGGCGCGGAGGTGAGCGCCGCGTCGAACCGGGCCAGGCCCGCATCCCCGGACATCGCGGCCGTGCCCGAGCGCGCCATCCGGGCCAGATCCGCGGTGGCGAGGTGTTCCGTCATCCCCTCGTCCACCGCCCACAGGCCCCAGGCCAGTGAGGTGGCCGGGCCGCCCGCGGCGCGGATGTGCTCGGCGAGGGCGTTGAGGAACGCGTTCGCCGCGCCGTAGTTGCCCTGTCCGCCGCTGCCCAGCACGGAGGCGGCGGACGAGAACAGGACGAAGGAGGCGAGGTCCGCGCCCCGGGTCAGCCGGTGCAGATGCCAGGCCGCGTCCACCTTCGGCGCCAGCACGGCGTCGAGCTGTTCGGGCGTCAGCGAGGTGACCACGCCGTCGTCGAGTACGCCCGCGGTGTGCACGACCGCGGTCAGCGGGTGCTCCTCGGGGACGGTGGCGAGCAGGGCGGCCAGCGCCTCGGGGTCGGAGGTGTCGCAGCTGACGATCCGTACGTCGGCCGCGAGCCCGGCCGCGAACGCCTCGATGCCCTCGGCGGCCGGCCCGCGCCGGCTCACCAGCAGCAGATGCCGGGCGCCATGCGCCTCGACGAGGTGGCGGGCGAGCAGCCGGCCGAGCGAGCCGGTGCCGCCGGTCACCAGCACGGTGCCGTCGGGGTCGAGCGCCGCCGGCTCCGCGTCGGGGTCGGGGTGCGCCGGCACCAGCCGGGGCACCAGCGCGCGCCCCTCGCGCAGCGCCAGCTGCCCCTCGCCGGCGGCCACGGCGGCGGCCACCGCCGCCGCCAGCGCGTCCCGGGACGCCGGGGCGTCGTCGGTGTCCACCAGCACGAGCCGCCCCGGGTGTTCGGCCTGGGCGGCGCGCAGCAGGCCCAGGACCGCCGCGCCCGCCAGGTCGGCCGGCGCGTCGTCCGGGCCGGCGGCCACGGCTCCGGCGGTGAGGACCACCAGCCGGCAGGCGCCGAGGGCGTCGTCCGCCAGCCACTCCCGCAGCCGCGCGAGGACGTGGCGGGTGGCGGCGCGTACCCGGGAGGGCGGTTCGTCGCCGCCCGACGTGTCGTACGGGGCGCCGAGGCAGGCCAGGACGGCCAGGTCGGGGGCCGGGGCGCCGGCGGCGACCGACGCGCTCAGCGCGGCCAGGTCCGGGTGGCGCGTGACGTGCTCCCCGGGCAGCGAGGCCGTGGCGGCGTCGGTGGCGAACGTCCCGCCCGTACGGCCAGCGCTGTCGGGCCCGTTCACGGCGTCCGTGGCGACCACGGCCCAGCGGTCCGGCGCCGGTACCGCGGGGGTGGGCGGCGCGAAGGCCTCCCAGTCCAGCCGGTACAGCGGCCTGTCCGCCGGGATCGCGCCGGACCGCCACCGCCGCGCGGAAACGGCGCGCAGCGTGAGCGAGTCGACGGCCACGACCTCGGCACCGGCGTCGTCGGACAGCCGCATGGTCACGGTGTCCGGCCCGGTCGGCGTGATACGGACCCGCAGCCTGGACGCGCCGCGCGCGAGCAGCCGTACGCCGCCGAAGGAGAACGGCAGCCGGACCGTGCCCGGTGCGCCGCCCGCCTGGTCCTCGCCGGTGAACAGCGCGCCGATCGGATGCAGCGCGGCGTCCAGGAGCGCGGGGTGGACGGCGTACGCCGCGCCGTCGCCCCGCCGGTCCGGGGCGAGGCTCACCTCGGCGAACATCTCCTCGCCGACCCGCCACGCGGCGCGCAGCCCGGTGAACGCGGGCCCGTACCGGTAGCCCTCGGCGGCCAGCCGCTCGTACAGCCCGGCCACGTCGATGGCCTCCGCCCCGGCGGGCGGCCACTGGGCCGGCTCCGCCGCGGGCTCG GTGACGCCGGTCCCCGCGCCGGCCGGCGCGGTGCCGAGCGTGGCGGTGGCATGCCGGCGCCACGGGCTGTACGGGCCGTCGCCCGACTCCTCCGGGACACCAGGGTCCGCCGAGCCGTCGCCGGACGGGCGCGAATAGACGGTGACGGCCCGGCGGCCGGCCGCGTCGGCGGCCCCGACGGCCACCTGGAGGTCCACCGCACCCACCGGAGGCAGCAGCAGCGGCGCCTCCAGGGTGAGATCGTCGATCCGGTCGCAGCCGGTCCGCTCGCCGGCCTGGAGGGCCAGCTCCACGAAGAGCGTGCCGGGCAGTGGCACGGCGTCCACGATGGCGTGGTCGGCCAGCCAGGGCTGGCTCCGCAGCGACAGCCGGCCGGTCAGCAGCAGCCCGCCGTCCGCCGGTTCGAGGGCTGCGCCCAGCAGCGGGTGGTCGCTCGCCTCGAGCCCCGCGGCCCGTACGTCCGCCTCCGCCTGCGGGGCGTCGCGCCAGTAGCGGCGCCGGTCGAAGCGGTAGGTGGGCAGGTCGGTGGCGCGGGCGCCGGGCGGGAAGGCGGCGGAGAAGTCGACGCTCTCGCCGTCCACATGGAGCCGGGCGAGCGCGGTCAGCAGCGTACGGGAGTCGTCGCGGCCCTTGCGCAGCACCGTGGCGAACACCGGCGCGCCGGGACCGGACCCCGGACCCGCACCCTGAGCCGATTCCCCGGCGGCTGCCCCGGGGGCCCCCTCCGCGGTCAGGCAGTCACGCGCCATGGTGGTGAGCACGGCGTCCGGGCCCAGCTCCAGGAAGCGGGTGACGCCCTCCGCGCGCAGCGCGGCGACGCCGTCGCGGAACCGCACCGGCTGCCGCGCGTGCCGCACCCAGTAGTCCGGGTCGCACAGCTGCTCGGCCCCGGCCAGGGCGCCGGTGACGTTCGAGACGACGGCGATCCGCGGCTGGGCGTACGTGAGCGAGGCGGCGACCCGCCGGAACTCCTCCAGCATCGGGTCGATCCGCGGCGAGTGGAAGGCGTGGCCGACCCGCAGCCGCCTGGTGCGCTGTCCCCGCTCCCGGATCGTACGGGCGATCTCCTCGACCACCTCCTCGTCGCCCGAGACCACCACCGACGTCGGCGCGTTGACGGCCGCCAGCGCCACCCGGTCCGCCCGTCCGGCGAGCAGCGGCGCCACCTCCTCCTCGGCGGCCTCCAGCGCGACCATCGCGCCGCCCTCGGGCAGCGCCTGCATCAGCCGCCCCCGGGCAGCCACCAGCCGCGCCGCGTCCGGCAGCGACAGCACCCCGGCGACATGCGCGGCGACCAGTTCGCCGACCGAGTGTCCCGCCAGATAGCCGGGGACCACACCGAACGACTCGACCAGCCGGTACAGGGCGACCTCGACCGCGAACAGCGCGGCCTGGGTCACCTCCGTCGCGTCCAGCGCGCCCGCCCCGGCCCCGTCTCCGTCGGCCGGTGCGAACACCAACTCCCGCAGCGACCGCCCCAGATGGGGGTCAAGCTGGGGGTCCAGGGCCGCACACGCCTCGTCGAACGCGGCGGCGAACACCGGGTGGGCCCGGTACAGCTGGGCGCCCATGCCCGGGCGCTGGCTGCCCTGCCCGGTGAAGAGGAACGCGCTGCCACCGAGCCGCGCCGGAGCGGACGCCCGCGTCACATCGGGGTGTGTCCGCCCTTCGGCGAGGTCGGCGAGCCGGGCCGACAACTCCTCGCGGTCCGCGCCCAGCACGACCGCGCGGTGGTCGAGCGGCGAGCGCGTGGTGGCCAGCGACCAGCCGATGTCGCGGGCCGGGACGTCCGGGTGCGCCGCCACGTGCTCGGCGAGCGCCGCGGCCTGCGTCCGCAGCGACGCCGCGCCGCGCGCGGACACCACCCACGGCACGGCGGTGCCCTCCGGCCGCTGAGGCCCGTTCTCGGGCAGCTCGGTGTCCGGCCGTGGCGGGGCCTCCTCCAGGATCAGATGCGCGTTGGTGCCGCTGATGCCGAAGGACGAGACACCGGCCCGGCGCCGCCGGTCCCCGGCCGGCCACGAGACCGGCTCGGCCAGCAACCGCACCGCGCCGCCGTCCCAGTCGACCACGGGCGTGGGCCGGTCGGCGTGCAGCAGGGCGGGCAGCGAGGCGTTCCGCAGCGCCATCACCATCTTGATCACGCCGGCGACGCCCGCCGCGCCCTGCGTGTGCCCGAGGTTCGGCTTGATCGCGCCCAGCCACAGCGGCCGCCCCTCGGGCCGCTCCTGACCGTAGGTGGCCAGCAGCGCCTGGGCCTCGATCGGGTCGCCGAGCGTGGTGCCCGTGCCGTGTGCCTCGACGGCGTCCACATCCGCCGGGGAGAGCCGGGCGCTTGCCAGCGCCTGCCGGATGACGCGCTGCTGCGACAGGCCGTTGGGCGCGGTCAGCCCGTTGCTCGCGCCGTCCTGGTTGATCGCCGAACCGCGCACCACGGCCAGCACCGGGTGCCCGTTGCGCTGGGCGTCCGACAGCCGCTCCAGCACCAGCAGCCCCGCACCCTCGGCGAAGGCCGTGCCGTCGGCCGCCTCGGAGAAGGCCTTGCACCGGCTGTCGGCCGCCAGGCCGCGCTGCCGCGCGAACTCCGTGAACACGTAAGGCGTGGTCATGACCGTCACGCCGCCCGCCAGCGCCAGCTCGCACTCGCCCTGCCGCAGCGCCTGAGCGGCCAGGTGCATGGCCACCAGCGACGACGAACACGCCGTGTCCACCGTGACCGCGGGCCCCTCAAGACCCAGGGTGTACGCCACCCGGCCGGACAGCACGCTCGGCGCGTTGCCGGTGACCAGATAACCCTCCGCGGCCCGGTCGATGTGCGGGGTGCCGAAGCCGGGCAGGGCGGTGCCCGCGTACACGCCGACGGGCGCGCCCTTGAGCGACCCGGGCGCGATGCCCGCCCGCTCCAGCGCCTCCCAGGCCACTTCGAGCAGCAGCCGCTGCTGCGGGTCGACCGCCGCCGCCTCGCGGGGGCTGATGCCGAAGAACTCCGGGTCGAACCGGTCGGCGTCGTGCAGGAATCCGCCCTCGCGGGCGTAGCTGGTGCCCGGGTGGTCGGGGTCCGGGTGGAACAGCCGCTCCAGGTCCCAGCCGCGGTTGTCCGGAAACGCGGTCATCGCGTGCCGGCGCGCGGCGACGAGGTCCCACAGGGCCTCGGGGGAGGCGACGTCGCCGGGGTAGCGGCAGGCCATCGCCACGATGGCCACCGGCTCCCGCTGCCCGTCCTCCACCTCGCGCAGCCGCGCCCGGGTGTCGTGCAGATCGGCGGCGACCCGCTTGAGATAGTCGACCAGCCGCTCTTCGTTGGTCTCAGTGGTCACGGCGCGTCACTTCGCCTTCGAGGTTTCTCGGGATTCTCGGCGTTCCTGGCAGACCGGGCTCATTGCACTCCGAGTTCCTTGTCGATGAAGTCCAGGACCTGGTCGGTCGTCGCGGCTTCGAGCCGTTCGGCCGCGGTGCCGCCGTTCCGGTGCGGGGCGTGCGCCGCCTTCCACGTGGCCAGCAGCGACTCCAGACGCGCCGTCACC GCGCCGGGGTCGGCCGCTGTGCTCGTACCGCCGTTCACCCCGGACGCGGTGAGGGCCGTCAGGGTCTCCTCCAGGCCCGTCAGCCGGCTGAGTACGGGCGCGTAGAGGTCCGGCGCCGTCGTCGCCCCCGCCTCGGGGGCGAGTTGGGCGCGCAGGTGCTCGGCCAGCGCCCCCGGCTCCGGGTAGTCGAAGATGACCGTGGCGGGCAGCCGCAGCCCGGTGGCGGCGGTGACGCGGTTGCGCAGCTCGACGGCGGTCAGCGAGTCGAACCCGAGCTCCTTGAACGCCGCGTCGGCCGGTACGGCCTCGGGGTCGGCGTGGCCGAGCACGGTGGCGGCGTGCCTGCGCACCAGGTCGGTGAGCAGGCGCCGCTGTTCGGCCGGGGCGAGGGCGCCCAGCCGGGCCGCCCAGTCCCCGGGCTCCTCGGCCGCCGCCGCGGTCGGCCGCGCCGCCGGGCCGGCCAGGCGGGCGCCCGCCTGGGCGTCCGCCGCCAGGGCGCGCAGCGGCGCGGGCAGGGCCGCGACGGGCTGCGCGGCCAGCGCCCGGGTGTCCAGGTTCCACGCGAGCAGCGCCGGCCGCCCGTGCCCGAGCGCCGCGTCGAACAGGTCCTGTCCCTGTTCGGTGCTGTTCGGGACGATGCCCTGCCGGGCGATGCGCGCCACATCGGCCTCGGTCAGCCCTCCGGTGAGCCCGCTCGCGGCGGACCACAGACCCCAGCTCACCGACACGCCCGGCAGCCCCTCGGCGTGCCGCCGCTCGACCAGGGCGTCGCAGTACGCGTTGGCGGCCGCGTAGTTGGCCTGCCCCGGGGTGCCCAGGGTGGACGCGAACGACGAGAACAGCACGAACATGGCCAGCGGCAGCCCGGCGGTCGCGCGGTGCAGACGGTCCGCCGCCGCGGCCTTGGCCGACCAGACCTCCGCCAGGCGCTCCTCGTCCTGCGAGGGCAGCATCGCGTCGGCCACCACGCCCGCGGCGTGGACGACGCCCGTGAGCGGATGCGCCGGGTCGACGCCCGCGACCGCCTCGTCCAGGGCGGTGGCGTCCCCGACGTCCACCGCGGCCACCCGCACCCGCGCGCCCAGTTCGGTGAGGCGGGCCGTCAGCTCCTCGGCGCCCGGGGCGCTCAGGCCGCGCCGGCTGAGCAGCAGCAGATGCCGTACCCCGTGCGAGCGCACCAGGTGTTCGGCGATCAGGCCGCCGAGGGTGCCGGTGCCGCCCGTGATCAGCACCGTGCCGTCCGGGTCCGGCGCCGGATCCACGTCAAGCACCAGTTTCCCGGTGTGCCGGGCCTGGCTGAGCCGCCGCAGCGCCTGCCTGGCCTGGCCGAGGCGCCACCCGGTGACGGGCAGGGGCGTGAACGCGCCGTCGGCGAACCGCTCGCCCAGGGCCGCCAGCAGCCGCCCGATGCGCTCCGGACCGGCGTCCGCGATCAGGTCGAACGCCCGGTAGGCGACCGAGTGTTCGTGGGCGACCTGATCGGGGTCGCGTACGTCCGTCTTGCCCATCTCGATCAGCCGCCCGCCGGGTGCGAGAAGCCTCAGCGAGGCGTCCGTGAACTCCCCGGCCAGGCTGTTGAGGACGACGTCGACGCCCCGGCCGCCGGTCGCCGACGAGAACGCCGCCTCGAAGCCCAGATCCCGCGAGGACGCCCGGTGGGCGGCGTCCACACCCATGCCCTCCAGCACCGGGTGCTTCGCCGGGCTCGCCGTCGCGAACACCTCCGCGCCCAGGTGCCGGGCGACCTGGACGGCGGCCATGCCCACGCCGCCGGCCGCACCGTGGACCACCACGGTCTCGCCGGAGCGCAGCCCGCCCAGCTCCACCAGGCCGTACCACGCGGTCAGGAACGCCACCGGGGCCGCCGCGGCCTCCCGGAAACTCCAGCCGTCCGGCACCGGGGCGACCATCCGGGCGTCCGCCACCGCCACCGGGCCGAACGCGTCGTCGAACAGGCCCATCACGCGGTCGCCGACGGCGATCCCGGCCACCCCCTCGGACACCTCCAGCACGACACCGGCGCCCTCGCTGCCGATCGACGCCGCCGCGTCCGGGTACATGTCCAGGGCGATCAGCGCATCGCGGAAGTTGAGCCCCGCGGCCCGTACGGCGATACGCACCTGGCCGGGCGCGAGGGGAGCCGAGGCCCGGGGGCTGGGCACCGCCGTGAGGTCGTCGGGGCCGGTCTTGCCGGCCACCACGCGCCACGCCCGCTCACCCGCCGGGGCCACCAGCTCCCGCTCGGCGTCCACCCGGGCCATCCGGGGCACCAACGCGCGCCCGCCACGCAGCGCGAGCTGCGGCTCGTCCGTACGCACCGCCTCCCGCAACGCGTCCCGCAATGCCTCCAGGCCGGTGTCGGCGTCGGCGTCGGTGTCGGTGTCGGTGTCGATGTCGATCAGGAGGAAACGCCCCGGGTGTTCCGTCTGGGCGCTGCGCACCAGCCCCCACACCGCGGCCGCCGCGGGGTCCACCCGCTCCGGGTCGCCGTCCCCGGTCCGCACCGCCGACCGGGTGACCACGGCGAGCCGCGCCCCCTCGACCCCGCTGCCGGCCAGCCACTCCTGGGCCAGCGCCAGCGTCCGCCGCGCGGCCGCCAAGCCGGTGTCCGCCTCCGCCGGGTCCGTACGCACCGGGGCGACGACGACATCCGGTGCGGTGGCTCCGCCGTCCACGGCGCGGAACAGCGCGTCGAGGTCGGGATAACGGTCGGCGCCGGCCAGGTCCGGGGCCTCGTCGCCCAGCACCGCGAGCGACGCGGGGGCCGTCCCGGACGCCGTCCCGCCCGCCGGGGCGGACAGCGGCGGCATCCATTCGACGGCGAACAGCCCGTGCGACCCCCGCACCCGGCTCGACGCGCGCAACTGCCGGACGCCCGTCTCACGGAGCGCGACCGCGTCCGCCGTCAGCACGGGGGCGCCCGTGGCGTCGGCCAGCAGCACCCGCAGCCCCTCCGGCCGCGGCGACAGCCGGACCCTGAGCCGGGTGGCGCCACCCGCCCACAGCCGCACCCCGCTCCAGGTGAACGGGAGCAGCACCGTCCCCGTCCCGGGCGCCGCGTCGACACCCGGCGCGGCGGCGAGCTGACCGGCCAGCAGTACGGGCTGCATCGCCGCGTCGAGCAGGGCGGGATGGATACCGAAGCCACCCGCCTGCTCCGCCGCCTCCTCGGGCAGCACCACCTCCGCCAGCGTGTCCTCGCCGTCGTGCCATACGTGGGCGAGGCCCCGGAAGAACGCGCCGTAGCCGTAACCAGCTGCCCCGGCCCGCTCGTAGAACCCCTCGATCGCCGCCGGCCGCGCCCCCGGCGGCGGCCACGCCCCGCCGAGACCGGGCGGCGGAACCGGCCCGGCCGCGCCGGGGAGCAGCGTGCCCGAGACATGGCAGACCCAGGTGTCCGGCGCCGCTGTGTCCTCCTCGGGCCGGGAGTAGACCCGCACCGGACGGCGCCCGTCGCCCTCGGCGGCGTCCACGACCACCTGGATCACCAGCCCGCCCGATGCGGGAAGCGCCACCGGGACCTGGAGCG CCAGCTCCTCGACACCGCCGCAGCCGGCCTCGTCGGCCGCCCGCAGCACCCATTCGACCAGCACCGCGCCGGGCAGCAACGGCAGGTCCGCGACCAGGTGTTCGGCCAGCCAGCCGCCCCGCGCCGCCTCCGGCAGCCGCCCGCTGAGCACCAGGCTCCCGTCGGCCAGGCGCACGGACACCGGGAGCAGCGCGTGTCCGACCGCGGTGAGCCCTGCGCCAGCGACATCGCCGGGACCGGAGCTCCCACCGGCCGGCAGCCAGTACCGCTCCCGCTGGAAGGCGTACACCGGAAGCGGCACCGCCGCGGGCGCGGGCCCCTCGGCGTACCAGCTCGCCCAGTCCACCGCCACGCCCGCGACATGCGCCTCGGCCAGCGCCCGGCACAGCCTCTCGGGGCCGCCCTCGTCCCGGCGGAGCGTGCCGATCGCCACGGCCGGGACGCCTGCCGCCTCGAAGCACTCCTGCAGGCCGAGCAGCAGCACGGGATGCGCGCTGACCTCGATGAACACCCGGTAGTCGGCGTCGAGCAGGGCCTGGACCGCCTCGGCGAAACGCACCGGCCGGCGCAGATTGGTGAACCAGTAGCCGGCGTCGAGACCGGAGGTGTCCATACGGGCGCCGGTCACGGTCGAGTAGAAGGCGGTGTCGGAGGAGGCGCCGGACACCCCGGCCAGCACCTCGGCCAGCTCGTCCGCGATGCCGTCCACCTGCGGGCCGTGCGAGGCGTAGTCCACGTCGATCGCGCGGGCCCGCAGCCCGTCGGCGCGCGCGGCGGCCACCAGCTTCTCGACCTGCTCGGGCGGCCCGGAGACCACCGTGGACGACGGGCCGTTGACCGCCGCGACCGCCACGTCCGGCGCGTGTGCGGCGGTCAGCCGCTCCGTCCCCTCGACACCGCAGCCCAGCGAGGCCATGGCGCCCTGCCCGGCCAGCGCCCGCAGCGCCCGCGCCCGCAGCGCCACCACACGGGCGCCGTCCTCCAGCGTCATCGCGCCCGCGACACAGGCCGCCGCGATCTCCCCCTGCGAGTGGCCGACCACGGCGGCCGGCCGTACGCCGTGCGCCTGCCACACCGCGGCCAGCGACACCATCACCGCCCACAACACCGGCTGCACCACATCGACCCGGCCGATGTCGGCCGCCCCCGGCGCGCCGCGCAGCACGTCGGTCAGCGACCAGTCGACATGCGCGGCCAGGGCCCGTTCGCACGCCGCGAGGCGCGCCGCGAAGACGGGGGAGGTGTCCAGCAGTTCCACGCCCATGCCGAGCCACTGCGACCCCTGCCCGGGGAACACCATGACCGGGCCCGCGCCCACGTCCGCCGCCACCCCGCGCACCACCGCGGGGCCCGGCTCGTCGGCCGCGAGACCGTCGAGCGCGGCGAGGAGGTCCGACCGCTCGCGGCCCACCACGACGGCCCGGTGCTCGAACGCCGAGCGGCGCGTCGCCAGCGACCAGCCCACGTCCGCGGCGCCACTCGCCGCACCGTCCGCCGTCAGAAAGGCGCCCAGCCGCGCGGCCTGGTCCCGCAGCGCCGCCTCGGTACGCCCCGAGACGGGCCAGGGCACCACGCCGTGGCGGCCGCCGTCCGCGGTGCCGGACGGGGCCGGTTCGGGCTGGTCCGGGGCCTGTTCGAGGATCACATGGGCATTGGTGCCGGACACGCCGAACGACGACACCGCCGCCCGGCGCGGCCGGTCGGTCACCGGCCAGTCGTGCGGGTCCGTCAGCAGCCGCACCGTGCCGGAGCTCCAGTCCACGTGGCGGGTCGGCTCGTCGATGTGCAGGGTCCTGGCCAGCTTGCCGTCCCGCAGCGCCATCACGGTGTTGATCGCGCCCGCGACCCCGGCCGCCGCGTGCGTATGGCCGATGTTCGACTTGATCGAGCCGAGGTACAGCGGCCGTTCCGCCGGGCGGTCCGCCCCGTACGTGGCGATCAGCGCCTCCGCCTCGATGGGGTCGCCCAGCCGGGTGCCCGTCCCGTGCGCCTCGACCATGTCCACGTCCGCGCCGGACAGCCCGGCCCTCGCCAGCGCGTCGCGGATCACGTTCTCCTGGGCGACGTCGTTGGGCGCGGTCAGCCCGTTGCTCGCGCCGTCCTGGTTGATGGCCGAGCCCCGCAGGACGGCCAGCACCTCGTGGCCGTTGCGCTGCGCGTCGGACAGCCGCTCCAGCAGCAGCAGGCCGGCCCCCTCCGAGAAACCGGTGCCGTCTGCGGCGGCGGCGAACGGCTTGCACCGGGCGTCGGGTGTGAGCGCCCGCTGCCGGCAGAACTCGGTGAACATCAGCGGCGTGGACAGCACCGTGACGCCGCCCGCCAGCGCCAGGGAGCACTCGCCCTGCCGCAGCGCCTGCCCCGCCAGATGGATGGCCACCATCGACGACGAGCACGCGGTGTCCACGGTGACCGCGGGGCCCTCGAAGCCGAAGGTGTACGCGACGCGGCCGGAGATGACGCTGGTCAGGCTGCCGGTGGTGGCGTAGCCCTCGAAGCCCTCCGGGATCCGCGGCAGTCCGGAGATGTAGTCCTCGCTGGACACCCCCACATACACACCGGTCCGGCTGGCGCGCAGCGCGCCGGGCACCACCCCGGCGGACTCCAGCGCCTCCCACGACAGCTCCAGGAGCAGCCGCTGCTGCGGGTCCATCGCCAGCGCCTCGCGCGGGCTGATGCCGAAGAACCCCGCGTCGAACCGGTCGACGTCGTACAGGAAGCCGCCCTGCCTCACATAGCTGGTGCCGAAGTGCGCCGGATCCGGGTGGAAGAGGCCGTCCAGGTCCCAGCCCCGGTCGGTGGGGAAGTCACCGATCGCGTCGCCGCCCGACAGGACGAGCCGGCGCAGGTCTTCGGGGGACCGCACCCCGCCGGGGAAACGGCAGGCCATGCCGACCACCGCGACCGGCTCCTGCTCACCCTCCTCCAGCTCCCGCAGCCGGGTCCTGGCGTCGTGCAGCTCGGTGGTGACCCGGCGCAGGTACTCGACCAGTTTCTCCTCGCTGACCATGTCGTCACCTGCCTTCAGTTGGAGGGGCGCGGACGCGCCTCGGAGAGCACGCCGAGCTCGTTGTCGATGAAGTCGAGGATTTCGTCGGCCGTGGCCGACGCGAGCTTGTCCGCCGCGTCGAGCGCCGGGCCGTTGCTGTTGTTCCCAGCGTTCTTTGTGGCGTTGTCCGCACCGTCGGCGTTGTTCGCACCGTCGGTGTTGTTCGTGCCGTCGGGCGCGAGCTGCTGCTCGATCCGGCGGGCCAGCGCCTCCGGCGTCGGATGGCGGAAGACGACGGCGGCGGGCAGCCGCAGCCCGCCGGCCGCCACCAGCCGGTCGCGCAGCTCGACGGCGGTCACCGAGTCGAAGCCCAGCTCCTTGAACGAGGCGTCGGCCCGTACGGCCTTGGGGTCGGGCTGCCCGAGCACGGCGGCGGCGTGATCGCGCACCAGCTCCAGGACGGCGCGGCGCCGTTCGGTCGGCCCGAGGCCCACCAGCCGGCTCGCCCAGTCGGTGGCGCGGCCGGGCGCGGCGGCGGCCACC GGCCGGACCGGCGCCCGGCCGGCCGACCCCGCGACGAGCGCGCGCAGCGCGGCCGGCAGCTCATGGACCGGGTGGGCGGTCAGGGCCCGGGTACGGATGTCGGCCGCCACCAGCCCCGGTCCGTCGGCGCGGCAGGCGGCGCGCAGCAGTGCGAACCCGCGGTCCGCGCCGAGGACCCCCGTCCAGCGCCCGCCGTCGTCGCCGTCGTCGTCGCCGCCGTCGGCATCGGCTGCGGCATCGGCTGCGGCCCAGGGCCCGAACGCCACCGCGAGGCCCGGCAGTCCGGCCCGGCGCCGTACGTCCATCACGGCCTCGCAGAACGCACCGGCCGCGGCCCTTTCGGGGGCGACCGGGCCGTCCCAGGCCGTGGCGGCGTCGGAGAGCGTCACGAACCGGACGGACGGCAGCCCGGCGGTGGCATGGTGCAGCGCCCGCACCGCCCCGGCCGCCACGGCCCACCCCCGCGCCGCGCCACGTGTCTCGATGTCGGCGGACAGCCCCGCGACATGGATGACGCCGGTCAGCGGATGGGCCGGATCGACCTGCGCCACGACCTGTTCGAGCGCGGCGGTGTCGCCAGGATCGACCATGACCACGCGTACGTGGGCGTCTTGCGTGCCGTACGCGTCGTCGGTGTCGTCCGTGCCGCCGAACCGTACGAGCAGCAGGTGGCGGACGCCCTCCGCGCGCACCAGCTGCTCGACGACCGCACCGGCGAGCGGGCCCCCGGCGTCCGTGACCAGGATCGTGCCCTCCGGGTCGAACGCCACGCCGCCCGAAGCGGCGGCGAAACGCGCCAGGCGTGGCGCGAGCAGCGTGTCTCCGCGCACCGCGACTGTCGGCTCACCGAGCGCGGCCGCCCGAGCCCCCACCGTGCCGACGTCGGTGCCGATGTCGGTGTCCGTGTCCACATCGACGAGGACGAAGCGGCCGGGGTGTTCCGCCTGGACACCACGCACATAGCCCCAGGCAGCCGCCGCGGCCGTGTCCACCGGCTCGCCCTCGGCGCCGCCCGCCGCGACCGCACACCCAGTGACCACCGCGAGACGGCTGCCCGCCAGCCGCGGTTCGCCGATCCAGGTGCGCAGCGCCGCCGCCACGCCCCGTACGGCGAACAGGGCGGCGTCCTCCGGCGCGGCGCCGGGGTCGGCGGTCGGTGGCCGCCACACCACCACGGAGGGCGCGGGCACGCCCGCGTCCAGGGCGGCCAGCAGCGCCGGGAGGTCCGCATACGTGTCCCGGCACCCGTCCGCGCCCGGCTCCGTCGGCGCCTCGCCCAGCACGGCCACACACCCGGCGTCGTCCTCGGTGCCGGACGGCTCCCGAGGCGTCACAGGAGTCCAGTCGAGGACGAAGAGACCACCCGCCGCGGCCCCGCTCGCCCCGCGCACCTGCCGTACGGTGCGCGGGCGCAGCACCACCGACGCCACGCTCAGCACGGGGGCCCCGGCCGGGTCGGACACCTCGATCCGCACCTCGCGATCGTCCCCGCCGTCCCCGTCCAGCGGCGTCAGCCGGACCCGCGCGGCGCGCGCCCCGGTCGCCCACAGACGGACGCCGCTCCACGCGAACGGCAGCCACAACCGGGCCTCCGGCCCCTCTCCCTCGCCCCCCGCGTCGTCCGGCTCGGTCAGCAGGGCGGGCTGCAACGCGGCGTCCAGCAGGACCGGGTGCAGACCGAATCCGGCCGAACCGCCGCCGTCTTCGCCACCCACGTCACCGGCCTCCTCGGGCAGCACCAGGTCGGCGACCAGCTCGGTCCCGTCCCGCCACAGCGCCGCCACTCCCCGCAGCGCGGGCCCGTAGCCGTAGCCGCGCCGCTCGGCCCGCTCGTACAGCCCGGCCGGCTCCAGCGCCTCGGCGCCCGCCGGCGGCCAGGGGGCGACCGGCGCCCCGGTGTCCGCCGGAGCGGGCGGGCCGCCCGGGACCAGCGACCCGACCGCGTGACACGTCCACCCCCCGCCCGCGGTGGCCTCGGCTGGCCGGGAGGAGATCCGCAGCTCACGGCGGCCCGACCCGTCCGGGGCCCCGACCGCGACCTGCGCCAGCACACCTCCGGGCGCTCCGGGCGCTCCGGGCGCACCGGATGCCCCGGGCAGCTCCAGCGGAGCGCGCAGCAGCAGTTCCTCCAGCGACGCCCCGCCCGCCTCGTCGGCGGCCAGCAGCGCCCAGTCCACCAGGACCGCGCCGGGCACCAACCGGACGCCCGCCACGGTGTGCGCGCCCAGCCAGGCCGCGTCGCCGGGGAGCCGGCCGCTGAGCACCAGGCCGCCGTCGGCCAGTTCGACCGCCGAGGACAGCAGGGCGTGCCCGGCCGGCCGGGTTCCGGCTCCGGCCGCACGCCCCTCGCGGCGGCCCTGGTCAGGCGCCAGCCAGTACCGCCGCCGCTGGAACGGGTAGGTGGGCAGCTCGACCGTACGCGGCCGGTGGCCCTCGTCGCCCCTGCCGCCCTCGTCGCCCGCGAACCAGCGGCTCCAGTCGACCGCGAGCCCCGCGGCGAAGCCGTCCCCCAGGGCGCGCGCCACTTGATCCGGGCCGCCCTGGTCCCGGCGCAGCGTCGGCACGGTGGCCGCCGCCACATCGGCCGCCTCGAAGCACTCCCGCAGCGCGGGGATCAGCACCGGGTGCGGGCTGACCTCGATGAACACGCGGTGTCCGGCGGCCAGCAGGGTGTCGATCACCGGGGCGAACCGGACCGGCCGGCGCAGATTGGTGAACCAGTAGTCGGCGTCCAGCTCCTCCGTCGCCTGCCGGGCGCCGGTGACGGAGGAGTAGAACACCGCGCCCGATCCACCGGGGGCGAGGTCCGCCAAGTCGGCGCGGATGGTGTCCGCGAGCCGGTCGACCTGCGGGCCGTGCGAGGCGTAGTCCACGTCGATCGCGCGGGCCCGGTGGCCCGCCGCCTCACACGCCGCCACCACCGCGGTGACCTGGTCGGTCGGCCCCGACACCACCGTGGAGAACGGCCCGTTGAACACCGCCACCGTCACCCGCGCGGCCGCCGCGCCCAGGTCCGCGAGGAAGCGCTCGGCCTCCTCGCCGCTCAGCGCGAGCGAGGCCATGGTGCCGTGGCCGGCGAGGGCGCGCAGGGCCCGGGAGCGCAGCGCCACGACCCTGGCGCCGTCCCGGAGCGAGAGGCCCCCGGCGACGCATGCGGCGGCGATCTCGCCCTGTGAGTGTCCGACGACCGCGGTGGGCTTCACCCCGAAGCTCTCCCAGACGGCGGCGAGCGACACCATCACCGCCCACAGACACGGCTGGAGTACATCGACCCGGGAGGGCTCGGGCGCGCCCGGCGCGCCGCGCAGCACGGCGGTGAGGGACCAGTCGACGAACTCGCCGAGCGCGGCCTCGCACGCGGCGACGCGGGCCGCGAAGGCGGGGGAGGAGTCCAGCAGTTCCACGCCCATGCCACGCCACTGCGACCCCTGCCCGGGGAACACCATGACCGGGCCGGGGGCGGACCCCGCCGGGACGGCGGCGGGCGCGGGGCCGTCCGCCACCGCGCGCAGCC CGGACAGCAGCTCGTCCCGGTCCGTACCGCTGACCACGGCCCGGTGGCGGTGCGCCGACCGCGTGGTGACCAGCGACCACCCCACGTCCGCCGGGGAGGGCGGGGCTTTGGCGGTGACCCACTCGGCCAGGCGCCCGGCCTGGGCACGCAGCGCGTCGGCGGTACGGGCCGACAGCACCCAGGGCACCACGCCGCCCGCGGCGGTGGCGGGCTCCGGTTCGGGCTGGTCGGGTGCTTGTTCGAGGATGAGGTGGGCGTTGGTGCCGGAGATGCCGAAGGAGGAGACGGCGGCGCGGCGGGGCCGGTCGGCCCCGGGCCAGTCGACGGGCTCGGTGAGGAGCCTGACCGTGCCGCCGGACCAGTCGATGTGCGGGCTGGGGGCGTCGATGTGGAGGCTGGCCGGGAGGCGGCCGTGGCGCAGGGCCATGACCATCTTGATGACGCCCGCGGCACCGGCGGCGGCCTGGGTGTGGCCGATGTTCGACTTGATGGACCCCAGCCACAGTGGCCGGTCGTCGGGGCGGTGCCCGCCGTAGGTGGCCAGGAGGGCCTGGGCCTCGATGGGGTCGCCCAGCGTCGTCCCGGTGCCGTGGGCCTCGACCGCGTCCACGTCCGCGGGGCCGAGGCGGGCGTTGGCCAGGGCCTGGCGGATGACGCGCTGCTGGGAGGGGCCGTTGGGGGCGGACAGGCCGTTGCTCGCGCCGTCCTGGTTGACCGCCGAGCCGCGGATCACGGCGAGCACCTGGTGGCCGTTGCGGCGCGCGTCGGACAGGCGTTCGAGCAGAACGAGCCCGGCGCCCTCGCTCCATCCCGTGCCGTCGGCGGCGGCCGCGAACGGCTTGCACCGCCCGTCCGGGGCCAGGCCGCGCTGCCGGGAGAACGAAGTGAACATGCCCGGCGTGGCCATCACCGTCACACCGCCCGCGAGCGCCAGATCGCATTCGCCCTGCCGCAGCGCCTGCGCGGCCAGGTGCATCGCCACCAGCGACGACGAACACGCCGTGTCCACGGTGAGCGCCGGTCCCTCCAGGCCGAGGGTGTAGGCCACCCGGCCGGACGCCACGCTCGGCGTGGAACCGGTCAGCAGATGTCCGTCGAGCTCGGCGGCGGCCTCGTGCAGCCTCGGCCCGTAGTCCGCCGCCGTCGCGCCCACGAAGACGCCGGTCGCGCTGCCGGCCAGCGAGGCCGGCGGGATCTGGGCCCGTTCCAGCAACTCCCAGGAGAGTTCGAGCAGAAGGCGCTGCTGCGGATCCATGGCCAGCGCCTCACGCGGGCTGATCCCGAAGAACTCCGCGTCGAAGTCCCCCGCGTCGTACACAAAACCGCCCCGGCCCGTCGCGCTGCCGCCGCTCCCGTCCGGTGCCCGGCCGAACAGTTCGGCCAGATCCCAGCCCCGGTCGCCGGGGAACTCGCCCACGGCGTCCACGCCTTCGTCCACCAGGCGCCACAGCGCCTCGCCGGAGTCGGCCCCGCCCGGGTAGCGGCAGCTGACGGCCACCACGGCGACCGGGTCATCGGCCGTGGCCGCCCCGACCGCCGCCGTCGGGGCGGTGGCCGGCTGCTCCTCGTGCGCCGCGCGCAGCCACGCCGCCACGGCGTTCGGCGTCGGATGGTCGAAGGTGAGGGTGGCGGGCAGTGCGGTGTCCGTGGCCGCGCCGAGGCGTTCGCTCAGCTCCGTCGCGGCCAGCGAGTCGAAGCCGAGCTGTTTGAAGGTCAGGGCCGGGTCGATGCCGTCGGGGCTGTCGTGCCCCAGGACGAGGGCGGCGTGGGTACGGACGAGCGCCAGCAGTTCGCCCACGGAGGCCGTGGCCGCGCGATCGACGGGCGGCGCAGCATCGGCCCGCGGCGCGGCATCGGCCCGCGGCGACGCCTCGGCCGGCGGCGCGACCGGTGAGATCCGGGTACGCCCCGCCCCTCGCCCGTCCAGCCCGTCGGGCCAGTGGCGTTCGCGCTGGAAGGCGTACGTCGGCAGGGGTACCCGCCGGGGCTCGTGGCCCGCGAAGGCGGCCGCCCAGTCGACGGCCGCGCCCCGTACGTGTGCCTGGGCCATCGCCGACACGAAGCTGTGCGCCTCCGGCCGGCCGCGGCGCTGCACCGCGACCGCCGTCGGACGAGGCGCGTCCCCGGCCCATGCGGCGGCGCTCTCCCGCGCCATGCCGGTCAACGAGGCGTCGGGGCCGAGTTCGAGGAAGGTGTCGACCCCCGCGTCGCACAGGTGCCGCACACCGGCGTCGAACCGTACGGCCTGCCGCGCGTGGTCGGCCCAGTAGTCGGGGGAGCGCAACTGGGCCTCGGTGGCCGGCCCGCCGGTCACATTGGAGACGACGGGGATGGCGGGCGCGGCGAACGTCAGCCCGGCCGCGACCGCGCGCAGCTCGCCGAGGATGCCGTCCAGGTGCGGGGAGTGGAAGGCGTGGCTGACCTTCAGCACGGTGGCCTTGCGGCCCCGCGCCCGCCAGCCGGCGGTCGCCTCCGCCACGGCCTCGCGGTCACCGGAGATCACCACGGAGGCGGGTGCGTTGACGGCCGCGAGGCCCACCCGCCCGCCGTGCGCCTCCAGCGCCTGCTCGGCCTCCTCGGCGGTGGCCTGCCAGGCGGCCATCGCCCCGGGGGTGGTGATGGACTGCATGAGGCGGCCCCGGGCCGCCACCAGCGTGCAGGCGTCGGGCAGCGACAGCACCCCGGCCACATGCGCGGCCGTGATCTCGCCGACCGAGTGGCCCATCAGGTGGTCGGGGGTCAGGCCCCAGGACGTCACCAGGCGGTAGAGGGCCACCTCGACGGCGAACAGCGCGGGCTGGGTGTACGCCGTCCGGTTCAGCGGCGGCTCACCGTCCGCGGGAGCCTCGGCGAACATGAGGTCCAGCAGCGGGGTGTCGGTGTCGAGCAAGGGGTCCAGGTGCCCGCACACCTCACGCAGCGCGTCGGCGAAGACGGGGTACGCCGTGTACAGCTCGCGGCCCATGCCGGGGCGCTGGCTGCCCTGGCCGCTGAACAGGAACGCGACCCGGCCCCCGCCCGCGCGGCCGCGGATCACCCCCGACGTCTGTGTCCCGGACGCCAACGCGTCCAGAGTGCCGTGCAGTTCGGCCACCCCCGTGCCCAGGACGACGGCTCGGTGGGGGAACGCCGTCCGGGTCGTGGCGAGCGCGTGGCCGACGTCCACCGGGTGGAGTCCGGGGTGTGCGCGCAGGTGTTCGCACAGCGCGGCGGCCTGGGCCGCGAGCGCCGTCGGGCTCGCCGCGGAGACGACCCACGGCGTGACCGGCGGGACGGTGCGCTTCGGCACGTCCCCGGGCGGCGTGGGCCGGTCCGACGGTGTGGCGGCGGCGGGCGGTTCGGCCAGGACGACATGGCAGTTGGTGCCGCCCATGCCGAAGGAGCTGACCCCGGCCGTGAGCCGCCGCCGCTCGTCGGGCCACGGCTCGAACGCGGTCTGGACGCGCAGCCCGAGCTCGGCCAGCGGGATGGCGGGGTTGGGGGTGGCGAAGTTGAGACTCGGCGGGAGCCGCCGGTGGTGGA TGCTCAGGGCCGTCTTGAGCAGGCCCGCGATCCCGGCGGCGGCCTCCAGGTGGCCCACGTTGGTCTTGACCGAGCCGACCCGCAGGGGGGCGGCCCGACGCTGGTCCTGGTGGTGCCCGCCGAGCGCGGCGCCGAGGGCCGCGGCCTCGGTCGGATCGCCGACCGGTGTGCCGGTGCCGTGCAGTTCGACGTACTGGAGTTCGGCGGGCGACACCGCGGCCCGCCGCCACGCCTGCCGCACCACATCCGTCTGGGCCTCGGCGCTCGGCCGGGTCAGGCCGTCGGTGGCGCCGTCGTTGTTGACCGCGCTGCCCTCGATCACGCAGTACACCGGGTCGCCGTCGTCGACGGCCGCGGCCAGCGGCTTGAGCAGCACCATTCCGCCGCCCTCGCCGCGTACGAAGCCGTTGGCGCGCGCGTCGAAGGTGTAGCACCGGCCGTCCGGCGACAGGCCCTGGAACTGGGCCTTCGCCATCCGCATGCTCTCCTCGGCCAGGATCAGGTTCACCCCGCCCGCGAGCGCCAGATCCGCCTCCCTGCGGCGCAGACTCTCGCAGGCCAGGTGTACGGCGACGAGCCCGGAGGACTGCGCCGCGTCCACCGTGAGGCTCGGGCCGCGCAGGCCCAGGAGGTGGGATATCCGGTTGGCGATGATGCCGCGGTGCAGGCCCGCCATCGTGTGCCGGGTGATGGCGATGTCGCTGGGCCCGTGCCGGTGGAGGAGCGTGGCGTAGTCGTCCCAGATCGCGCCGGTGAACACCGCGGTCCGGCACCCCGCAAGGTTCCCGGGGCCGAGCCCCGCGTCCTCCATGGCCTCCCAGGCCAGTTCGAGCATCAGCCGCTGCTGCGGATCCATCGCCGTGGCCTCGCGCGGCGAGATGCCGAAGAATCCGGCGTCGAAGGTGTCGATGTGCTCCAGTAGCCCGGCGAAGTACGGCGCGGAACCGGGCTCGGCGGACAGGCGGTCGTCCGGGACCGGGACGACGGCGTTCTCGCCCGCGCGCAGCAGGCGCCAGAACGCCTCGGGATCGGGTGCGCCGGGCAGCCGGCAGGCCAGCCCCACGATCGCGACGGGAGACGCCGCCTGCTGCGCGTGGCTCGGGCTGCTCCCGGCCATGACACCCCCATACCTGAATCGCGAAACCGCTGGCTATGGCTTACGAGATGCCCCGAACAGCGCTCATTCTCCGAATGTTTTCTAAAGTTGATCTAACCGGGAATGTCTGGGGAGCTCCTTGTATACGCCGGGCGAAACTTGACCCGCTGATGCTTCGGATCAGAGCGGTGACCTGCGTGGGGCCGCCACCGAAACGGCAGGGTACGGTGACGGTCACCGATCGACGAATCACCCTATGTGCCGATTTGGTTGTGGGTCTATTGACCCTCACACTGGCGCGCGCCGCACAGTTAGGATGCGTTGATGTTGCCCTCTCTGCGATCACTGCTGCTGGTTGTTCGCCCTTCGGAGAGGACCGCGGCCCGTGATGCCGGGCGCAGTTCGGCACCATGAGTCGGCGCAGCCGTCGATCGCAGAGCAACGCCGTCGATCTCCGTAGCCAGAAGACCACCTCCCATCTGACCGAAAACCTCACTGGGGGCGATGTTGCAGCCATGTGAAATGCGCGGAGGTGGCTGCGATCTGGCTAAGCTGTCGGTGGCGTGGGTAGGCATCGACGCTGAGGTCTGAAGCGCGACCATAAAGGCTTCCAAAATCGGGGTGAATTTTTCGGATGCTGGCCGAATTCGATCACAATGGAGCGCACTCCTGGTTCGACACGACGCCGGCGATGGGGGGAGTTCGCGTATCATGAGGTACGAGATACTGGGCCCGTTGCGCGTGGTTGACGGATCTTCGGTCACCTCAATCAGTGCCCGCAAGATCGAGGTGCTGCTGGCGGCATTACTCGTGCGGGCCGACGAGGTGGTGAGCAGCGAAGATCTGGTGAAGGAGATCTGGGGCACCCGGGCGCCGCGGCAGGCCGTGGCGGCGCTCCACGTCTATGTGTCCCAACTCCGCAAGTTCCTCAGGCGCCCCAACCGCACCGAGAGCCCCATCGTGACCGCTCGCAGCGGGTATGTGCTGCGTCTCGGCGCGGACGAGCTGGACTTCCATGTCTTCCAGGGGCTGGTGCGCCAGGGGCGGGCCGCACAGCGGGCCGGGCGCACCCATGAGGCCTGCGAGGCTTACGAAGAGGGCCTTGACCTCTGGCGCGGCCCGGTCCTCGAGGACCTGCGCGACGGGGCGATCGTCAACGGCTTCGTGTGCTGGGCCGAGCAGGCCCGGCTGGAATGCGTCGAGTCGTTCGTGGACGCCGGCCTGGAGCTCGGCCGCCACCGTGAGTTCGTCAGCTACCTCTACGCCCGGATAGAGGAGCTTCCGCTGAACGAGGCGTTCTACCGGCAGCTCATGCTGGCGCTCTACCGTTCGGGCTGCAGGGCCGACGCCCTGAGTGTCTACCAGTCGGCGCGGACCGTGCTGAGCGAGGAACTCGGGCTTGAGCCGTCCGCCGCCCTCAAGCGGTTGCACCATGCCGTGCTCATCGACGCCCCGGAACTCGAAGCCGCCTAGCCGAATCGCGCGCACCGCCGACTCGTCACGCATCCAGGAACAGCAGAACAGGGGGTAATTCGTGCGGTACGAGATTTTGGGACCTCTCCGTGTAGTCGACGAAAAGGGCGGTTCGCCGCTCAGAGCGCAGAAACTCGCTACCGTTCTCGCCGTCCTGCTGATCCGCTCGGATCAGGTCGTGATGGCGGAACAGCTCACCAGAGAGATCTGGGGCGAGCTGCCGCCTCGGCGCGACACCGCGAGTATCTACGTCTGCATTTCGCGGCTGCGCAAGTTCCTGAGCCGTTCGGGCCAGCAGAATCCCATCATCACCCGCCCGGGTGGATATATGCTGCGCAGGGCGTCGAGCGAATGCGATTTCGACGCCTTCCAGCAGCTGGTCACCGACGGGCGGAAGTACGTCAGATACGAGGAGCCCGCGCAGGCGGCGCTGTGTTTCGAACAGGCCCTGGCGCTGTGGCGGGGCCCGGCGTTCGGGAATGTCCAGACGGGACCGATCGTGACCGGTTTTCTTAAGCGGCTGGACGAGATGCGTATTGAATGTGCTGAGATGTCGGCCGAGGCGTATCTCGAACTCGGCCGCCATCGGGAACTCGTCGGCCGGCTGCACTCCCTCGTCGCCGAGTTCCCGCTCCGGGAGACGTTCTACCGACAGCTGATGCTGGCCCTCTACCGCTCCGACCGGAAGTCCGAGGCGCTTGGCATATACGAGTCCGCCAGACGTGTTCTCGACAGGGAACTGGGGCTTGAACCCGGCCGGAAACTCAGGGAGCTCCGGCACACGGTTCTCGCCTCGACCTGACGGACCGCGCCTGGCGGACTCGTTCTGCAGGCCACCGCCCGAGCTCGCCGGGGGTTCGACTCGCACATCTGCGAGTGAACCCGCCGGCGAAGACCGGGCCCTGGCCGCGTGGCTGATTACTCGGCGACATACGTCCGGCGGATCCACTCGAAGACCCGCAGATGCCGCCCGTGGTGCTCCATGA ACTGGCCTGTGGGCTCGAAGTGTTCGTACGCGTTGCCGATGAGGAGCTTCAGGGTCGACTCGGTGTCATCGACGTACCGGGTGTGCGGATCGGCCAGCTCCGTGGGGCCGCCGAGCAGGATGACATTGGGCGCAGATGCGGACATAAAAGGCTGCATTCCCTTCGCTATACACCCAAGAGAAGGTTGGCATGAGGCGGAAAGCGCGCCGATGGGCGGCATACTTCTTGCTCGCAAAGGGCTTGTGGGGGCCCGACGTGGGCGGGCTTCCAGATCATCCCGATCTGCGCCCCCAAGCCACTGCGCTCGGTCCAGCCTAGACGCGCCGGGTCCTCCGGTGTGAGGGTCGTTCGACCGACAAAAGGCTTCGATTTCCTCACGGATCGTGAGGTCACCTCTACCGCCCCGCCACATCGATGGCCGCCGAGGATGGATGCCCATGCCGATTCGCTCACTGTCGCCCGGTATCGACCCGCCGGCCGCGGCGCGCCTTCCCGGCTCGGGAGGGCCCTGGGCGCGTGAATACGTCGCGTTCAACCGGCGGTTGGCGCTCTACGGGCGCGCCGGGGTGATGAGCGCGTGCGCCGTGCTGGGCGTGGTGGCCATGCCGGTGGACGAGATGGCGCCCACGGCGGTGGTCGCGGCGGCCGTGCTCGCCTGGAGCTGGATCCACCTGCGGCTGGCCACCACCCCGGCGATGCCGCCGAGGTCGCTGCTCACCCTGGACCTGGCGGTCATGACGGCGCTGTGTCTGAGCCAGCGGTTCACCATCCCCGAGACGCAGACCACGCACGGGGGCACATGGGTCATGGTGTGCGTCAACTTCACGGCCGTGGCGTACCAGCTGATGCAGCCGCTGCGCACCGCGATGCTGGCGACGCTGTGGCTGTGCTGCGCGGATGTGGTGGGCGCCGCGCTGCACCCGCACCTCCAGTGGCCCGGCGTGCTGCGCAGTGTCATCTGGGTGGTGGTGAACACCGTCCTGGCCCGGGCCGTGGTCCGGCTGGTGTTCAGCGAGAGCCGGGCGGCCGACGACGCCGCCGACCGGGCCGCGCGGGCACGGCGGCACGGGGAGGCGGCGGAGGCCCGCTGCGCCGCGGAGCGGGAGCACCTGGCCGCGCTCCATGACACCGCCTGCGCGACCCTGCTGATCGCGTCGGCGCCGTGGGCGTCGATGCGGACCGAGACACTGCGGGCCCAGGCCGCCAGAGACCTCGTGCGGCTGCGCGCCGAGCAGCCGGTGGCGGGGGAGGTGGACCTGGCCGCCGAACTGCTGGACGAGATCGTCGCGCACCCCCTCCGCGTCATCAGCAGATTCGACGAGGACCTGGGCACGGCCTGGCGCCCCGTCGCGGCGGCCCTGCGCGGCGGTCTCGGCGAGGCGCTGCGCAACGTGGCCCGCTACGCGGGCGTCGAGGTCGTACGGGTGACGGCGCACCGCGCGGGGGAGGTGATCGTCGTGACCATCGCCGACCACGGGGTGGGGTTCGACCCCGAGCGCATCCCCACCCAGCGGATCGGGATCAAGCGTTCGATCCGCGGACGGATGTGGAACGTGGGGGGCCGCGCCACGGTGGAGTCCCGGCCCGGACACGGTACGACGGTGCGGCTGGAGTGGCCGCGTGGGTGAGGACTTCCGTCAGCTGATCACCCGTCATCAGCTCCGCGGCATCCGCGTCGGGGGGCTGCTCATCCTGGTCACCCTCCACATCGGGGCCGCCCTGCCCGGCCTGCTCGGCGCTCTGGACGACTACCGGCACCCCTTTGTCCCCCTCGCCGCCTACGGACTGCTCACGCTGGTCCTCGGCGGCAGCGTCGTCCTCGGCCTCGACGGGCGCCCATGGCCGCGCGGCTGGGTGCCGGCGGCGCTGACCGGCGTCTTCGCGGCCTCGGTGGCCACCACCACCCAGGTCCCGGCGGACCATTACTTCCTCACCACCTCCCACTGGTCCTACTCCCTCGCGGGCTGGTTCGCCGTCGTCCTGCTCGCCGACCGGGGGCCGGTGGTCTCCGGCGTCTTCCTCGGCTGCCATCTGGCCGTCACCGGCGCCCTGTTGCTGACGGTCCAGGTGCCCTCGCGGCACACCGGAGCGAGCATGGCCCTGAGCGTCCTCTCCGCCTGCTGCTTCCAGATGATCATCGCCGCCGGCGCGAAGCTCCTCCTCGACAGCAGCAGAGCCATCGGCGAGGCCCTGCGGGCCCAGGAGCGGGTGCGCACCCGTATCGCCGTCGCCCGGCAGATCCAGGCGGACCAGCGCCGCCGGTACGCCGAGCTGAACACCACCGTGCTCCCCCTGCTCACCGGCCTCGCCAGCGGCTCCCTCGACCCGGAGGACGACGAGGTGCGGCGCGCCTGCGCGTTGGAAGCCGCCCGGCTGAGGCGGCTGTTCGCGGAGAGCGACTGTACGTTCGACCCGCTGGTCCACGAGATGCGCGCCTGCATCGACGTCGCCGAGCGCAACGGCACCACCGTGCAACTCGCCGTCCGCGGCGACCCCCTCGAACTGCCGCTGCCCCTGCGCCGCGCGCTCCTGGACCCGGTCATCGCCGCCCTCGCCGCCGCCGGGCAGACGGCGCGCGTCACCGTCGTCCGCGGCGGGGATCAGGTGCGGGTGGGCATCGTGGTGGACGCGCTGCAGGACCGGCCGCCCGCGCCCGCGGCCAACGGGATCCGGGTGCGCACGGTCCACGCCGAGGACCGTCTCCTCGTGGAGGCCGCGTGCCGGATCACAGCTCGTCCAGAGCTATCAGCCCGTCCTGAACCGCCCTGGCCACCAGTTTGGCCTTCGTGGTCGCCGGGCGGCCGGCGTTCGCGTACTTGATGCGCACCCGGTCCAGGTAGGTGTTCACCGTGCGCTCGGAGATCCCCAGGCTCTGGGCGACCACGGCCTTCGACTCCGACTGGAACCACTCCACCAGCACCTCCACCTCCCGTACGGACAGCACGGGCCGGCTCTGCCGGGTGTCGCTGCCGAAGGCGCCGGCCAGCGACGGGGGCGTATAGGGGATGTCCTCAGCGGCGGCCCGGATCGCCTTGACCAGGTGCCGCTGCCCCTCCGCCTTGGTGATGTAGGTGGCCGACCCCAGATCCATGCAGGTCAACGCCGCCTCCTGGCTGTCCCGCATGGTATACACCACGACCTTGCGCTCCTGGGCCACGAGCTCCTGCAGCTCGTCGTACGCGGGCCGCCCGTTCTGCAACAGCAGATCCATGACCACGACATCCGCCCCCCGCCCCGGCCCGTGGAGCGCCACCGACACGTTCGCCCCCTCAGCCACCACCTTGATCGGCGCGTCGGCGGCGGCACACCACGCCGAGATGCCCGCGCGCACCAC(SEQ ID NO：36)
the explanation of the biosynthetic gene cluster is given by taking the biosynthetic gene cluster of the streptomyces as an example, and the biosynthetic gene cluster comprises a linear polyketide synthase gene module I, a glycosyl synthesis related gene module and a modifying gene module. The modules of the biosynthetic gene cluster of Streptomyces are described in detail below, specifically as follows:
wherein, the I type linear polyketide synthase gene module comprises 11 genes of 4A1, 4A2, 4A3, 4A4, 4A5, 4A6, 4A7, 4A8, 4A9, 4A10 and 4A11, and the specific details are as follows:
according to an embodiment of the invention, the 4a1 gene encodes SEQ ID NO: 1, as shown below. According to the embodiment of the present invention, 4A1 is located at 94189 th and 102849 th bases of the gene cluster nucleotide sequence, is 8661 base pairs in length, encodes polyketide synthase, and is 2886 amino acids in length.
Figure DEST_PATH_GDA0000919991810000341
Figure DEST_PATH_GDA0000919991810000351
According to an embodiment of the invention, the 4a2 gene encodes SEQ ID NO: 2, the amino acid sequence is specifically shown as follows. According to the embodiment of the present invention, 4A2 is located at 87491-94174 bases of the gene cluster nucleotide sequence, is 6684 base pairs in length, encodes polyketide synthase, and is 2227 amino acids in length.
Figure DEST_PATH_GDA0000919991810000352
Figure DEST_PATH_GDA0000919991810000361
According to an embodiment of the invention, the 4a3 gene encodes SEQ ID NO: 3, as shown below. According to the embodiment of the invention, 4A3 is located at 75306-87431 bases of the gene cluster nucleotide sequence, is 12126 base pairs in length, encodes polyketide synthase and is 4041 amino acids in length.
Figure DEST_PATH_GDA0000919991810000362
Figure DEST_PATH_GDA0000919991810000371
According to an embodiment of the invention, the 4a4 gene encodes SEQ ID NO: 4, as shown below. According to the embodiment of the invention, 4A4 is located at 63502-75285 bases of the gene cluster nucleotide sequence, has the length of 11784 base pairs, encodes polyketide synthase and has the length of 3927 amino acids.
Figure DEST_PATH_GDA0000919991810000372
Figure DEST_PATH_GDA0000919991810000381
Figure DEST_PATH_GDA0000919991810000391
According to an embodiment of the invention, the 4a5 gene encodes SEQ ID NO: 5, as shown below. According to the embodiment of the invention, 4A5 is located at 51523-63456 bases of the gene cluster nucleotide sequence, is 11934 base pairs in length, encodes polyketide synthase and is 3977 amino acids in length.
Figure DEST_PATH_GDA0000919991810000392
Figure DEST_PATH_GDA0000919991810000401
According to an embodiment of the invention, the 4a6 gene encodes SEQ ID NO: 6, as shown below. According to the embodiment of the invention, 4A6 is located at 46481-51502 bases of the gene cluster nucleotide sequence, has the length of 5022 base pairs, encodes polyketide synthase and has the length of 1673 amino acids.
Figure DEST_PATH_GDA0000919991810000402
Figure DEST_PATH_GDA0000919991810000411
According to an embodiment of the invention, the 4a7 gene encodes SEQ ID NO: 7, as shown below. According to the embodiment of the present invention, 4A7 is located at 32045-37036 bases of the gene cluster nucleotide sequence, is 4992 base pairs in length, encodes polyketide synthase, and is 1663 amino acids in length.
Figure DEST_PATH_GDA0000919991810000412
According to an embodiment of the invention, the 4A8 gene encodes SEQ ID NO: 8, as shown below. According to the embodiment of the invention, 4A8 is located at 6858-17204 bases of the gene cluster nucleotide sequence, has the length of 10347 base pairs, encodes polyketide synthase and has the length of 3448 amino acids.
Figure DEST_PATH_GDA0000919991810000413
Figure DEST_PATH_GDA0000919991810000421
Figure DEST_PATH_GDA0000919991810000431
According to an embodiment of the invention, the 4a9 gene encodes SEQ ID NO: 9, the amino acid sequence is specifically shown below. According to the embodiment of the present invention, 4A9 is located at base 23772-.
Figure DEST_PATH_GDA0000919991810000432
According to an embodiment of the invention, the 4a10 gene encodes SEQ ID NO: 10, as follows. According to the embodiment of the invention, 4A10 is located at 30776-31090 bases of the gene cluster nucleotide sequence and is 315 base pairs long, and the ACP protein of the coding polyketide synthase is 104 amino acids long.
Figure DEST_PATH_GDA0000919991810000433
According to an embodiment of the invention, the 4a11 gene encodes SEQ ID NO: 11, the amino acid sequence is specifically shown below. According to the embodiment of the invention, 4A11 is located at 17217-23720 base of the gene cluster nucleotide sequence, has the length of 6504 base pairs, codes polyketide synthase and has the length of 2167 amino acids.
Figure DEST_PATH_GDA0000919991810000434
Figure DEST_PATH_GDA0000919991810000441
The glycosyl synthesis related gene module comprises 9 genes of 4G1, 4G2, 4G3, 4G4, 4G5, 4G6, 4G7, 4G8, 4M and 4G9, and the specific steps are as follows:
according to an embodiment of the invention, the 4G1 gene encodes SEQ ID NO: 12, as shown below. According to the embodiment of the invention, 4G1 is located at 38367-39179 th base of the gene cluster nucleotide sequence, has a length of 813 base pairs, and encodes glucose-1-phosphate: TTP thymidine transferase, 270 amino acids in length.
Figure DEST_PATH_GDA0000919991810000442
According to an embodiment of the invention, the 4G2 gene encodes SEQ ID NO: 13, as shown below. According to the embodiment of the present invention, 4G2 is located at 39176-.
Figure DEST_PATH_GDA0000919991810000443
According to an embodiment of the invention, the 4G3 gene encodes SEQ ID NO: 14, as shown below. According to the embodiment of the present invention, 4G3 is located at 40211-41515 bases of the gene cluster nucleotide sequence, is 1305 base pairs in length, encodes NDP-D-glucose-3, 4-dehydratase, and is 434 amino acids in length.
Figure DEST_PATH_GDA0000919991810000444
Figure DEST_PATH_GDA0000919991810000451
According to an embodiment of the invention, the 4G4 gene encodes SEQ ID NO: 15, as shown below. According to the embodiment of the present invention, 4G4 is located at 41512-42567 bases of the gene cluster nucleotide sequence, has a length of 1056 base pairs, and encodes NDP-D-glucose-4, 6-dehydratase, NDP-D-glucose-4-isomerase, and NDP-D-glucose-4-reductase having a length of 351 amino acids.
Figure DEST_PATH_GDA0000919991810000452
According to an embodiment of the invention, the 4G5 gene encodes SEQ ID NO: 16, as follows. According to an embodiment of the present invention, 4G5 is located at 37128-38153 bases of the gene cluster nucleotide sequence, is 1026 base pairs long, encodes a glycosyltransferase, and is 341 amino acids long.
Figure DEST_PATH_GDA0000919991810000453
According to an embodiment of the invention, the 4G6 gene encodes SEQ ID NO: 17, as shown below. According to an embodiment of the present invention, 4G6 is located at 3271-4455 bases of the gene cluster nucleotide sequence, is 1185 base pairs in length, encodes a glycosyltransferase, and is 394 amino acids in length.
Figure DEST_PATH_GDA0000919991810000454
According to an embodiment of the invention, the 4G7 gene encodes SEQ ID NO: 18, as shown below. According to an embodiment of the present invention, 4G7 is located at 1084-2829 bases of the gene cluster nucleotide sequence, is 1746 base pairs in length, encodes a glycosyltransferase, and is 581 amino acids in length.
Figure DEST_PATH_GDA0000919991810000455
According to an embodiment of the invention, the 4G8 gene encodes SEQ ID NO: 19, as follows. According to the embodiment of the invention, 4G8 is located at 1 st to 1062 nd bases of a gene cluster nucleotide sequence, is 1062 base pairs in length, encodes endoglucanase and is 353 amino acids in length.
Figure DEST_PATH_GDA0000919991810000456
Figure DEST_PATH_GDA0000919991810000461
According to an embodiment of the invention, the 4M gene encodes SEQ ID NO: 20, as follows. According to the embodiment of the present invention, 4M is located at 42591-43523 bases of the gene cluster nucleotide sequence, is 933 base pairs in length, encodes methyltransferase and is 310 amino acids in length.
Figure DEST_PATH_GDA0000919991810000462
According to an embodiment of the invention, the 4G9 gene encodes SEQ ID NO: 21, as shown below. According to the embodiment of the present invention, 4G9 is located at 43764-45002 bp of the gene cluster nucleotide sequence, is 1239 bp in length, encodes endoglucanase, and is 412 amino acids in length.
Figure DEST_PATH_GDA0000919991810000463
Wherein, the modifying genes comprise 5 genes of 4O, 4I, 4E, 4P1,4P2 and 4P3, and the specific genes are as follows:
according to an embodiment of the invention, the 4O gene encodes SEQ ID NO: 22, as shown below. According to the embodiment of the invention, 4O is located at 29301-30725 bases of the gene cluster nucleotide sequence, has the length of 1425 base pairs, codes for epoxide oxidase and has the length of 474 amino acids.
Figure DEST_PATH_GDA0000919991810000464
According to an embodiment of the invention, the 4I gene encodes SEQ ID NO: 23, as shown below. According to the embodiment of the invention, 4I is located at 28271-29212 bases of the gene cluster nucleotide sequence, is 942 base pairs in length, encodes ketosteroid isomerase and is 313 amino acids in length.
Figure DEST_PATH_GDA0000919991810000465
According to an embodiment of the invention, the 4E gene encodes SEQ ID NO: 24, as shown below. According to the embodiment of the present invention, 4E is located at bases 31192-32043 of the gene cluster nucleotide sequence, is 852 base pairs in length, encodes epoxide hydrolase, and is 283 amino acids in length.
Figure DEST_PATH_GDA0000919991810000466
Figure DEST_PATH_GDA0000919991810000471
According to an embodiment of the invention, the 4P1 gene encodes SEQ ID NO: 25, as follows. According to the embodiment of the present invention, 4P1 is located at 5594-6790 bases of the gene cluster nucleotide sequence, is 1197 base pairs in length, encodes cytochrome P450 protein, and is 398 amino acids in length.
Figure DEST_PATH_GDA0000919991810000472
According to an embodiment of the invention, the 4P2 gene encodes SEQ ID NO: 26, as follows. According to the embodiment of the present invention, 4P2 is located at 26247-27536 bases of the gene cluster nucleotide sequence, is 1290 base pairs in length, encodes a cytochrome P450 protein, and is 429 amino acids in length.
Figure DEST_PATH_GDA0000919991810000473
According to an embodiment of the invention, the 4P3 gene encodes SEQ ID NO: 27, as shown below. According to the embodiment of the present invention, 4P3 is located at 45120-.
Figure DEST_PATH_GDA0000919991810000474
According to an embodiment of the present invention, the biosynthetic gene cluster further comprises: a regulatory gene module comprising: 4R1 gene, 4R2 gene, 4R4 gene, 4T1 gene, 4T2 gene, 4T3 gene, 4T4 gene and 4T5 gene, wherein the sequence information of the genes is as follows:
according to an embodiment of the invention, the 4R1 gene encodes SEQ ID NO: 28, as follows. According to the embodiment of the invention, 4R1 is located at 104363-105103 base of the gene cluster nucleotide sequence, is 741 base pairs in length, encodes a regulatory protein, and is 246 amino acids in length.
Figure DEST_PATH_GDA0000919991810000475
According to an embodiment of the invention, the 4R2 gene encodes SEQ ID NO: 29, as follows. According to the embodiment of the invention, 4R2 is located at 103540-104301 th base of the gene cluster nucleotide sequence, is 762 base pairs in length, encodes a regulatory protein and is 253 amino acids in length.
Figure DEST_PATH_GDA0000919991810000476
According to an embodiment of the invention, the 4R4 gene encodes SEQ ID NO: 30, as shown below. According to the embodiment of the present invention, 4R4 is located at 4415-5365 bases of the gene cluster nucleotide sequence, is 951 base pairs in length, encodes a transcription regulatory factor, and is 316 amino acids in length.
Figure DEST_PATH_GDA0000919991810000481
According to an embodiment of the invention, the 4T1 gene encodes SEQ ID NO: 31, as shown below. According to an embodiment of the invention, the 4T1 gene is located in SEQ ID NO: 36, the length of the gene sequence fragment is 1365 base pairs, and the gene sequence fragment encodes membrane integrated transfer protein and has the length of 454 amino acids.
Figure DEST_PATH_GDA0000919991810000482
According to an embodiment of the invention, the 4T2 gene encodes SEQ ID NO: 32, as shown below. According to an embodiment of the invention, the 4T2 gene is located in the Streptomyces strain SEQ ID NO: 37, the length of the gene sequence fragment is 687 base pairs, and the gene sequence fragment encodes ABC transfer factor and has the length of 228 amino acids.
Figure DEST_PATH_GDA0000919991810000483
According to an embodiment of the invention, the 4T3 gene encodes SEQ ID NO: 33, as follows. According to the embodiment of the present invention, 4T3 is located at 108013 and 108621 bases of the gene cluster nucleotide sequence, is 609 base pairs in length, encodes a two-component feedback regulator, and is 202 amino acids in length.
Figure DEST_PATH_GDA0000919991810000484
According to an embodiment of the invention, the 4T4 gene encodes SEQ ID NO: 34, as shown below. According to the embodiment of the invention, 4T4 is located at 106908-108107 bases of the gene cluster nucleotide sequence, is 1200 base pairs long, encodes a chemoreceptor, and is 399 amino acids long.
Figure DEST_PATH_GDA0000919991810000485
According to an embodiment of the invention, the 4T5 gene encodes SEQ ID NO: 35, as follows. According to the embodiment of the invention, 4T5 is located at 105725-106915 bases of the gene cluster nucleotide sequence and is 1191 base pairs in length, encodes a two-component sensor histidine kinase and is 396 amino acids in length.
Figure DEST_PATH_GDA0000919991810000486
Figure DEST_PATH_GDA0000919991810000491
Referring to FIG. 1, according to an embodiment of the present invention, along A3 '-5' end of a gene, the 4T3 gene, the 4T4 gene, the 4T5 gene, the 4R1 gene, the 4R2 gene, the 4A1 gene, the 4A2 gene, the 4A3 gene, the 4A4 gene, the 4A5 gene, the 4A6 gene, the 4P3 gene, the 4G9 gene, the 4M gene, the 4G9 gene, the 4A9 gene, the 4E gene, the 4A9 gene, the 4O gene, the 4I gene, the 4P 9 gene, the 4A9 gene, the 4P 9 gene, the 4R 9 gene, the 4G9 gene, the 4A9 gene, the 4G9 gene, the 4A9 gene, the 4G9 gene, the 4 gene, the 9 gene, and the 4 gene, The 4T1 gene and the 4T2 gene are sequentially connected in series.
In the present invention, "tandem" merely indicates the order of connection of genes, and adjacent genes may be directly connected to each other, or other gene fragments may be present or partial sequences of the connection sites may overlap with each other.
According to some embodiments of the invention, the 4T3 gene, the 4T5 gene, the 4a1 gene, the 4a2 gene, the 4A3 gene, the 4a4 gene, the 4a5 gene, the 4a6 gene, the 4P3 gene, the 4M gene, the 4G4 gene, the 4G3 gene, the 4G5 gene, the 4P2 gene, the 4R4 gene, the 4G7 gene, and the 4T2 gene are expressed in the same direction.
According to some embodiments of the invention, the expression direction of the 4T4 gene, the 4R1 gene, the 4R2 gene, the 4G9 gene, the 4G2 gene, the 4G1 gene, the 4a7 gene, the 4E gene, the 4a10 gene, the 4O gene, the 4I gene, the 4a9 gene, the 4a11 gene, the 4a8 gene, the 4P1 gene, the 4G6 gene, the 4G8 gene, and the 4T1 gene is the same.
Microorganisms
According to another aspect of the present invention, there is provided a microorganism. According to an embodiment of the invention, the microorganism encodes the biosynthetic gene cluster described above. The inventors have surprisingly found that the J1001-4 compound can be synthesized efficiently, simply and at low cost by performing a fermentation treatment using the microorganism.
According to an embodiment of the invention, the microorganism is streptomyces. According to a particular embodiment of the invention, the microorganism is Streptomyces endomyces subtype Streptomyces.
According to yet another aspect of the present invention, there is provided a process for preparing a compound of J1001-4. Referring to fig. 2, the method is explained according to an embodiment of the present invention, the method including:
s100 fermentation treatment
According to an embodiment of the present invention, the fermentation treatment is performed using the aforementioned microorganism to obtain a fermented microorganism.
According to a specific embodiment of the invention, the fermentation medium conditions are: soluble starch (30g/L), soybean cake powder (10g/L), yeast extract (2.5g/L) and calcium carbonate (3 g/L). Therefore, the fermentation effect is good, and the yield of the J1001-4 compound is high.
According to a particular embodiment of the invention, the fermentation treatment is carried out for a period of 7 to 8 days.
S200 wall breaking treatment
According to the embodiment of the invention, the fermented microorganism is subjected to wall breaking treatment to obtain a solution containing the J1001-4 compound.
S300 purification
According to an embodiment of the present invention, the J1001-4 compound is purified from a solution containing the J1001-4 compound to obtain the J1001-4 compound.
According to some embodiments of the invention, the solution containing the compound J1001-4 is spin dried, extracted with ethyl acetate, concentrated, and then separated by silica gel column and further purified by semi-preparative HPLC to give the desired product.
According to the method for producing the J1001-4 compound of the embodiment of the present invention, the J1001-4 compound can be efficiently synthesized by performing the fermentation treatment using the aforementioned microorganism. Then, the pure J1001-4 compound can be obtained efficiently, simply and at low cost through microbial wall breaking treatment and separation and purification treatment.
According to some embodiments of the invention, the biosynthetic pathway of the J1001-4 compound is predicted from genetic predictions as shown in fig. 3. On one hand, long-chain fatty acid chains are finally formed by catalyzing the extension synthesis of propionyl coenzyme A and methylmalonyl coenzyme A by a polyketide synthesis gene cluster, and glycosyl is obtained by the modification of 4O, 4I and 4E genes and obtaining an intermediate product of J1001-4, on the other hand, glycosyl is obtained by catalysis of genes such as 4G1, 4G2, 4G3, 4G4 and 4M, and the glycosyl is transferred onto the fatty acid chains by glycosyltransferases 4G5, 4G6, 4G7 and 4G9, and finally J1001-4 is formed by the action of P450 enzymes 4P1,4P2 and 4P 3. .
The present invention is described below with reference to specific examples, which are intended to be illustrative only and are not to be construed as limiting the invention.
The scheme of the invention will be explained with reference to the examples. It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention. The examples do not specify particular techniques or conditions, and are carried out according to techniques or conditions described in literature in the art (for example, refer to molecular cloning, a laboratory Manual, third edition, scientific Press, written by J. SammBruker et al, Huang Petang et al), or according to product specifications. The reagents or apparatus used are not indicated by the manufacturer, but are conventional products available commercially, for example from Illumina.
Example 1
Streptomyces endomyces subtype Streptomyces is inoculated to an SFM plate and cultured for about three days to four days. When colonies are formed in the SFM culture medium, selecting single colonies to a seed culture medium for culture for about three days to four days, inoculating the single colonies to a fermentation culture medium according to the amount of 1 percent (the components of the fermentation culture medium are 30g/L of soluble starch, 10g/L of soybean cake powder, 2.5g/L of yeast extract, CaCO 3 3g/L, pH 7.2). The culture conditions are as follows: 30 ℃ and 220 rpm, and culturing for 7 to 8 days.
And when the culture is carried out for 7 th or 8 th day, collecting fermentation liquor, separating the supernatant from the mycelium by high-speed centrifugation, adding the same amount of acetone into the mycelium to break the bacteria, carrying out ultrasonic treatment for 20min, carrying out rotary evaporation on the acetone, extracting the supernatant and the mycelium by using the same amount of ethyl acetate, and repeating the steps once. Concentrating to dryness by rotary evaporation, then carrying out crude separation by using a silica gel column to obtain a crude product detected by using HPLC, and considering that the HPLC result shows that the yield of the strain is higher, carrying out TLC plate spotting on a concentrated sample, developing in an iodine cylinder, removing a very obvious point, dissolving by using methanol, carrying out high-speed centrifugation, taking a supernatant, and carrying out mass spectrometry to determine the position of a target point, wherein the result is shown in figure 4, and the compound is a J1001-4 compound.
Example 2
The sequence information of the biosynthetic gene cluster of the J1001-4 compound is researched by taking Streptomyces endous subtype Streptomyces as a research object, and the specific method comprises the following steps:
1. extraction of DNA
(1) Streptomyces endomyces subtype Streptomyces is inoculated into a TSBY culture medium for 2 days, and after the culture, the strain is collected at 7000rpm × 5 min.
(2) The collected cells were discarded from the supernatant, and 30ml of SET buffer was added thereto, and the mixture was dispersed and centrifuged at 7000rpm for 5 minutes. The supernatant was discarded again, 10ml of SET buffer was added, the cells were disrupted, 300. mu.l of lysozyme (100mg/ml) was added, and the mixture was subjected to 37 ℃ water bath for 30 to 60 minutes, 100. mu.l of proteinase K (100mg/ml), 10. mu.l of RNase (10mg/ml) and 37 ℃ water bath for 30 minutes.
(3) To the mixture obtained in step (2), 1.2ml of 10% SDS was added, and the mixture was incubated at 55 ℃ for 2 hours while shaking gently every 15 minutes until the liquid became clear.
(4) 4ml of 5M NaCl was added to the liquid obtained in step (3), mixed well and cooled to 37 ℃. 10ml of chloroform was added, gently mixed for 30 minutes, and centrifuged at 12000rpm for 15 minutes.
(5) And (4) adding 10ml of chloroform into the centrifuged supernatant in the step (4), gently mixing for 30 minutes, and centrifuging at 12000rpm for 15 minutes.
(6) And (4) taking the supernatant centrifuged in the step (5), adding 0.8-time volume of isopropanol, and gently swirling until filamentous DNA appears.
(7) And (3) picking out the DNA in an EP tube, adding 70% ethanol for washing, then pouring out the ethanol, naturally drying, dissolving in a certain amount of double distilled water, and extracting to obtain the DNA of the streptomycete.
2. DNA sequence analysis
The genome was sent to a sequencing company to perform genome sequencing to obtain genome information, and the assembled genome was predicted for the gene encoding the protein using GeneMarkS. Then, an anti SMASH software is utilized to predict a region similar to a J1-001 compound synthetic gene cluster, and a J1001-4 synthetic related gene is excavated by combining the comparison result of proteins in the J1-001 compound synthetic gene cluster in the bacterial coding gene data set. The structure of the gene cluster is shown in FIG. 2, and is responsible for the biosynthesis of compound J1001-4, and the functional analysis of each gene is shown in Table 1.
Figure DEST_PATH_GDA0000919991810000501
Figure DEST_PATH_GDA0000919991810000511
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
While embodiments of the invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.
Figure IDA0000919991860000011
Figure IDA0000919991860000021
Figure IDA0000919991860000031
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Claims (4)

1. A biosynthetic gene cluster of a compound, wherein the compound has the structure,
Figure FDA0003295668660000011
wherein the biosynthetic gene cluster comprises:
the I type linear polyketide synthase gene module comprises: 4A1 gene, 4A2 gene, 4A3 gene, 4A4 gene, 4A5 gene, 4A6 gene, 4A7 gene, 4A8 gene, 4A9 gene, 4A10 gene and 4A11 gene,
wherein, the 4A1 gene codes SEQ ID NO: 1;
the 4A2 gene encodes SEQ ID NO: 2;
the 4A3 gene encodes SEQ ID NO: 3;
the 4A4 gene encodes SEQ ID NO: 4;
the 4A5 gene encodes SEQ ID NO: 5;
the 4A6 gene encodes SEQ ID NO: 6;
the 4A7 gene encodes SEQ ID NO: 7;
the 4A8 gene encodes SEQ ID NO: 8;
the 4A9 gene encodes SEQ ID NO: 9;
the 4A10 gene encodes SEQ ID NO: 10;
the 4A11 gene encodes SEQ ID NO: 11, and the amino acid sequence shown in the specification
A glycosyl synthesis-associated gene module comprising: 4G1 gene, 4G2 gene, 4G3 gene, 4G4 gene, 4G5 gene, 4G6 gene, 4G7 gene, 4G8 gene, 4M gene and 4G9 gene; and
a modifier gene module comprising: 4O gene, 4I gene, 4E gene, 4P1 gene, 4P2 gene and 4P3 gene,
wherein the 4G1 gene encodes the amino acid sequence of SEQ ID NO: 12;
the 4G2 gene encodes SEQ ID NO: 13;
the 4G3 gene encodes SEQ ID NO: 14;
the 4G4 gene encodes SEQ ID NO: 15;
the 4G5 gene encodes SEQ ID NO: 16;
the 4G6 gene encodes SEQ ID NO: 17;
the 4G7 gene encodes SEQ ID NO: 18;
the 4G8 gene encodes SEQ ID NO: 19;
the 4M gene encodes SEQ ID NO: 20;
the 4G9 gene encodes SEQ ID NO: 21, or a pharmaceutically acceptable salt thereof, wherein (A) is represented by the amino acid sequence shown in 21,
the 4O gene encodes SEQ ID NO: 22;
the 4I gene encodes SEQ ID NO: 23;
the 4E gene encodes SEQ ID NO: 24;
the 4P1 gene encodes SEQ ID NO: 25;
the 4P2 gene encodes SEQ ID NO: 26;
the 4P3 gene encodes SEQ ID NO: 27, or a pharmaceutically acceptable salt thereof.
2. The biosynthetic gene cluster of claim 1, further comprising:
a regulatory gene module comprising: 4R1 gene, 4R2 gene, 4R4 gene, 4T1 gene, 4T2 gene, 4T3 gene, 4T4 gene and 4T5 gene,
wherein, the 4R1 gene codes SEQ ID NO: 28;
the 4R2 gene encodes SEQ ID NO: 29;
the 4R4 gene encodes SEQ ID NO: 30;
the 4T1 gene encodes SEQ ID NO: 31;
the 4T2 gene encodes SEQ ID NO: 32;
the 4T3 gene encodes SEQ ID NO: 33;
the 4T4 gene encodes SEQ ID NO: 34;
the 4T5 gene encodes SEQ ID NO: 35.
3. The biosynthetic gene cluster of claim 2, wherein along the 3 '-5' end of a gene, the 4T3 gene, the 4T4 gene, the 4T5 gene, the 4R1 gene, the 4R2 gene, the 4A1 gene, the 4A2 gene, the 4A3 gene, the 4A4 gene, the 4A5 gene, the 4A6 gene, the 4P3 gene, the 4G9 gene, the 4M gene, the 4G4 gene, the 4G3 gene, the 4G2 gene, the 4G1 gene, the 4G5 gene, the 4A7 gene, the 4E gene, the 4A10 gene, the 4O gene, the 4I gene, the 4P2 gene, the 4A9 gene, the 4A11 gene, the 4A8 gene, the 4P1 gene, the 4R4 gene, the 4A 36 6 gene, the 4G 36 6 gene, the 4A2 gene, the 4G4 gene, the 4A4 gene, the 4P 4 gene, and the 4G 365952 gene, The 4G7 gene, the 4G8 gene, the 4T1 gene and the 4T2 gene are connected in series in sequence.
4. The biosynthetic gene cluster of any of claims 1 to 3, wherein the nucleotide sequence of the biosynthetic gene cluster is as set forth in SEQ ID NO: shown at 36.
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