CN106916836B - Biosynthetic gene cluster of compounds and application thereof - Google Patents

Biosynthetic gene cluster of compounds and application thereof Download PDF

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CN106916836B
CN106916836B CN201511000174.9A CN201511000174A CN106916836B CN 106916836 B CN106916836 B CN 106916836B CN 201511000174 A CN201511000174 A CN 201511000174A CN 106916836 B CN106916836 B CN 106916836B
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刘然
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Wuhan Hesheng Technology Co ltd
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Abstract

The invention discloses a biosynthetic gene cluster of a compound and application thereof. Wherein, a biosynthetic gene cluster of a compound, said biosynthetic gene cluster comprising: the I type linear polyketide synthase gene module comprises: 3a1 gene, 3a2 gene, 3A3 gene, 3a4 gene, 3a5 gene, 3a6 gene, 3a7 gene, 3A8 gene, 3a9 gene, 3a10 gene and 3a11 gene; a glycosyl synthesis-associated gene module comprising: 3G1 gene, 3G2 gene, 3G3 gene, 3G4 gene, 3G5 gene, 3G6 gene, 3G7 gene, 3G8 gene, 3M gene, and 3G9 gene; and a modifier gene module comprising: 3O gene, 3I gene, 3E gene, 3P1 gene, 3P2 gene and 3P3 gene.

Description

Biosynthetic gene cluster of compounds and application thereof
Technical Field
The invention relates to the field of bioengineering, in particular to a biosynthetic gene cluster of a J1001-3 compound and application thereof, and more particularly relates to a biosynthetic gene cluster of the J1001-3 compound, a microorganism and a method for preparing the J1001-3 compound.
Background
Polyketides are one of the most abundant groups of natural products, and are widely applied to the fields of medical use, veterinary use, agriculture and the like due to the great medicinal value of the polyketides. Such as the antibiotic erythromycin, the antiparasitic antibiotic abamectin, etc. Polyketides are formed under the catalysis of polyketide synthase (PKS), and gene clusters assembled in a modular form determine the structure and thus the biological activity of the polyketides, so that the gene cluster of a new compound has great significance for the subsequent research.
With the increasing severity of the drug resistance problem of pathogenic bacteria, the clinical need for new antibiotics is more urgent. In order to deal with the situation, people improve the existing antibiotics on one hand and accelerate the step of screening novel antibiotics on the other hand. The J1001-3 compound is a recently discovered antibiotic, encoding good bacteriostatic activity.
To date, no study related to the biosynthesis gene cluster of the J1001-3 compound has been reported.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art. To this end, it is an object of the present invention to propose a biosynthetic gene cluster encoding antibiotic-J1001-3 compounds with antibacterial activity.
According to one aspect of the present invention, the present invention provides a biosynthetic gene cluster of a J1001-3 compound, wherein the J1001-3 compound has the following structure,
Figure BDA0000889460040000011
according to an embodiment of the invention, the biosynthetic gene cluster comprises:
the I type linear polyketide synthase gene module comprises: 3a1 gene, 3a2 gene, 3A3 gene, 3a4 gene, 3a5 gene, 3a6 gene, 3a7 gene, 3A8 gene, 3a9 gene, 3a10 gene and 3a11 gene;
a glycosyl synthesis-associated gene module comprising: 3G1 gene, 3G2 gene, 3G3 gene, 3G4 gene, 3G5 gene, 3G6 gene, 3G7 gene, 3G8 gene, 3M gene, and 3G9 gene; and
a modifier gene module comprising: 3O gene, 3I gene, 3E3E gene, 3P1 gene, 3P2 gene and 3P3 gene.
The inventors have surprisingly found that the biosynthetic gene cluster of the J1001-3 compounds of the examples of the invention can control the biosynthesis of the J1001-3 compounds.
According to an embodiment of the invention, the 3a1 gene encodes SEQ ID NO: 1; the 3A2 gene encodes SEQ ID NO: 2; the 3A3 gene encodes SEQ ID NO: 3; the 3A4 gene encodes SEQ ID NO: 4; the 3A5 gene encodes SEQ ID NO: 5; the 3A6 gene encodes SEQ ID NO: 6; the 3A7 gene encodes SEQ ID NO: 7; the 3A8 gene encodes SEQ ID NO: 8; the 3A9 gene encodes SEQ ID NO: 9; the 3A10 gene encodes SEQ ID NO: 10; the 3A11 gene encodes SEQ ID NO: 11, or a pharmaceutically acceptable salt thereof.
According to an embodiment of the invention, the 3G1 gene encodes SEQ ID NO: 12; the 3G2 gene encodes SEQ ID NO: 13; the 3G3 gene encodes SEQ ID NO: 14; the 3G4 gene encodes SEQ ID NO: 15; the 3G5 gene encodes SEQ ID NO: 16; the 3G6 gene encodes SEQ ID NO: 17; the 3G7 gene encodes SEQ ID NO: 18; the 3G8 gene encodes SEQ ID NO: 19; the 3M gene encodes SEQ ID NO: 20; the 3G9 gene encodes SEQ ID NO: 21, or a pharmaceutically acceptable salt thereof.
According to an embodiment of the invention, the 3O gene encodes SEQ ID NO: 22; the 3I gene encodes SEQ ID NO: 23; the 3E gene encodes SEQ ID NO: 24; the 3P1 gene encodes SEQ ID NO: 25; the 3P2 gene encodes SEQ ID NO: 26; the 3P3 gene encodes SEQ ID NO: 27, or a pharmaceutically acceptable salt thereof.
According to an embodiment of the present invention, the biosynthetic gene cluster further comprises: a regulatory gene module comprising: 3R1 gene, 3R2 gene, 3R4 gene, 3T1 gene, 3T2 gene, 3T3 gene, 3T4 gene and 3T5 gene.
According to an embodiment of the invention, the 3R1 gene encodes SEQ ID NO: 28; the 3R2 gene encodes SEQ ID NO: 29; the 3R4 gene encodes SEQ ID NO: 30; the 3T1 gene encodes SEQ ID NO: 31; the 3T2 gene encodes SEQ ID NO: 32; the 3T3 gene encodes SEQ ID NO: 33; the 3T4 gene encodes SEQ ID NO: 34; the 3T5 gene encodes SEQ ID NO: 35.
According to the embodiment of the present invention, along the 5 '-3' end of the gene, said 3T3 gene, said 3T4 gene, said 3T5 gene, said 3R1 gene, said 3R2 gene, said 3A1 gene, said 3A2 gene, said 3A3 gene, said 3A4 gene, said 3A5 gene, said 3A6 gene, said 3P1 gene, said 3G5 gene, said 3M gene, said 3G4 gene, said 3G1 gene, said 3G9 gene, said 3A7 gene, said 3E gene, said 3A10 gene, said 3O gene, said 3I gene, said 3P2 gene, said 3A9 gene, said 3A11 gene, said 3A8 gene, said 3P3 gene, said 3R4 gene, said 3G6 gene, said 3G7 gene, said 3G8 gene, said 3T1 gene, and said T2 gene are connected in series.
According to an embodiment of the invention, the biosynthetic gene cluster encodes SEQ ID NO: 36.
According to another aspect of the present invention, there is provided a microorganism. According to an embodiment of the invention, the microorganism encodes the aforementioned biosynthetic gene cluster. The inventors have surprisingly found that the J1001-3 compound can be synthesized efficiently, simply and at low cost by performing a fermentation treatment using the microorganism.
According to an embodiment of the invention, the microorganism is streptomyces.
According to yet another aspect of the present invention, there is provided a process for preparing a compound of J1001-3. According to an embodiment of the invention, the method comprises: carrying out fermentation treatment by using the microorganism so as to obtain the fermented microorganism; carrying out wall breaking treatment on the fermented microorganisms to obtain a solution containing the J1001-3 compound; and purifying the J1001-3 compound from the solution containing the J1001-3 compound so as to obtain a pure product of the J1001-3 compound.
According to the method for preparing the J1001-3 compound of the embodiment of the invention, the J1001-3 compound can be efficiently synthesized by performing fermentation treatment using the aforementioned microorganism. Then, the pure J1001-3 compound can be obtained efficiently, simply and at low cost through microbial wall breaking treatment and separation and purification treatment.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
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The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 shows a schematic diagram of the sequence of biosynthetic gene cluster for the J1001-3 compound according to one embodiment of the present invention;
FIG. 2 shows a schematic flow diagram of a process for preparing a J1001-3 compound according to one embodiment of the present invention;
FIG. 3 shows a schematic flow diagram of the biosynthetic pathway of the J1001-3 compound according to one embodiment of the present invention;
FIG. 4 shows a schematic map of a J1001-3 compound according to one embodiment of the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements throughout, or elements encoding the same or similar functions. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, which are for convenience of description of the present invention only and do not require that the present invention must be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
It should be noted that the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to implicitly indicate the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. Further, in the description of the present invention, "a plurality" means two or more unless otherwise specified.
According to one aspect of the present invention, there is provided a biosynthetic gene cluster of the J1001-3 compound. According to an embodiment of the present invention, the biosynthetic gene cluster includes:
the I type linear polyketide synthase gene module comprises: 3a1 gene, 3a2 gene, 3A3 gene, 3a4 gene, 3a5 gene, 3a6 gene, 3a7 gene, 3A8 gene, 3a9 gene, 3a10 gene and 3a11 gene;
a gene module associated with glycosyl synthesis, the gene module comprising: 3G1 gene, 3G2 gene, 3G3 gene, 3G4 gene, 3G5 gene, 3G6 gene, 3G7 gene, 3G8 gene, 3M gene, and 3G9 gene; and
a modifier gene module comprising: 3O gene, 3I gene, 3E gene, 3P1 gene, 3P2 gene and 3P3 gene.
The inventors have surprisingly found that the biosynthetic gene cluster of the J1001-3 compounds of the examples of the invention can control the biosynthesis of the J1001-3 compounds.
According to an embodiment of the present invention, the biosynthetic gene cluster (also referred to as simply "gene cluster" in the present invention) is a biosynthetic gene cluster of streptomyces, encoding the amino acid sequence of SEQ ID NO: 36, specifically as follows:
GTGGTGCGCGCGGGCATCTCGGCGTGGTGTGCCGCCGCCGACGCGCCGATCAAGGTGGTGGCTGAGGGGGCGAACGTGTCGGTGGCGCTCCACGGGCCGGGGCGGGGGGCGGATGTCGTGGTCATGGATCTGCTGTTGCAGAACGGGCGGCCCGCGTACGACGAGCTGCAGGAGCTCGTGGCCCAGGAGCGCAAGGTCGTGGTGTATACCATGCGGGACAGCCAGGAGGCGGCGTTGACCTGCATGGATCTGGGGTCGGCCACCTACATCACCAAGGCGGAGGGGCAGCGGCACCTGGTCAAGGCGATCCGGGCCGCCGCTGAGGACATCCCCTATACGCCCCCGTCGCTGGCCGGCGCCTTCGGCAGCGACACCCGGCAGAGCCGGCCCGTGCTGTCCGTACGGGAGGTGGAGGTGCTGGTGGAGTGGTTCCAGTCGGAGTCGAAGGCCGTGGTCGCCCAGAGCCTGGGGATCTCCGAGCGCACGGTGAACACCTACCTGGACCGGGTGCGCATCAAGTACGCGAACGCCGGCCGCCCGGCGACCACGAAGGCCAAACTGGTGGCCAGGGCGGTTCAGGACGGGCTGATAGCTCTGGACGAGCTGTGATCCGGCACGCGGCCTCCACGAGGAGACGGTCCTCGGCGTGGACCGTGCGCACCCGGATCCCGTTGGCCGCGGGCGCGGGCGGCCGGTCCTGCAGCGCGTCCACCACGATGCCCACCCGCACCTGATCCCCGCCGCGGACGACGGTGACGCGCGCCGTCTGCCCGGCGGCGGCGAGGGCGGCGATGACCGGGTCCAGGAGCGCGCGGCGCAGGGGCAGCGGCAGTTCGAGGGGGTCGCCGCGGACGGCGAGTTGCACGGTGGTGCCGTTGCGCTCGGCGACGTCGATGCAGGCGCGCATCTCGTGGACCAGCGGGTCGAACGTACAGTCGCTCTCCGCGAACAGCCGCCTCAGCCGGGCGGCTTCCAACGCGCAGGCGCGCCGCACCTCGTCGTCCTCCGGGTCGAGGGAGCCGCTGGCGAGGCCGGTGAGCAGGGGGAGCACGGTGGTGTTCAGCTCGGCGTACCGGCGGCGCTGGTCCGCCTGGATCTGCCGGGCGACGGCGATACGGGTGCGCACCCGCTCCTGGGCCCGCAGGGCCTCGCCGATGGCTCTGCTGCTGTCGAGGAGGAGCTTCGCGCCGGCGGCGATGATCATCTGGAAGCAGCAGGCGGAGAGGACGCTCAGGGCCATGCTCGCTCCGGTGTGCCGCGAGGGCACCTGGACCGTCAGCAACAGGGCGCCGGTGACGGCCAGATGGCAGCCGAGGAAGACGCCGGAGACCACCGGCCCCCGGTCGGCGAGCAGGACGACGGCGAACCAGCCCGCGAGGGAGTAGGACCAGTGGGAGGTGGTGAGGAAGTAATGGTCCGCCGGGACCTGGGTGGTGGTGGCCACCGAGGCCGCGAAGACGCCGGTCAGCGCCGCCGGCACCCAGCCGCGCGGCCATGGGCGCCCGTCGAGGCCGAGGACGACGCTGCCGCCGAGGACCAGCGTGAGCAGTCCGTAGGCGGCGAGGGGGACAAAGGGGTGCCGGTAGTCGTCCAGAGCGCCGAGCAGGCCGGGCAGGGCGGCCCCGATGTGGAGGGTGACCAGGATGAGCAGCCCCCCGACGCGGATGCCGCGGAGCTGATGACGGGTGATCAGCTGACGGAAGTCCTCACCCACGCGGCCACTCCAGCCGCACCGTCGTACCGTGTCCGGGCCGGGACTCCACCGTGGCGCGGCCCCCCACGTTCCACATCCGTCCGCGGATCGAACGCTTGATCCCGATCCGCTGGGTGGGGATGCGCTCGGGGTCGAACCCCACCCCGTGGTCGGCGATGGTCACGACGATCACCTCCCCCGCGCGGTGCGCCGTCACCCGTACGACCTCGACGCCCGCGTAGCGGGCCACGTTGCGCAGCGCCTCGCCGAGACCGCCGCGCAGGGCCGCCGCGACGGGGCGCCAGGCCGTGCCCAGGTCCTCGTCGAATCTGCTGATGACGCGGAGGGGGTGCGCGACGATCTCGTCCAGCAGTTCGGCGGCCAGGTCCACCTCCCCCGCCACCGGCTGCTCGGCGCGCAGCCGCACGAGGTCTCTGGCGGCCTGGGCCCGCAGTGTCTCGGTCCGCATCGACGCCCACGGCGCCGACGCGATCAGCAGGGTCGCGCAGGCGGTGTCATGGAGCGCGGCCAGGTGCTCCCGCTCCGCGGCGCAGCGGGCCTCCGCCGCCTCCCCGTGCCGCCGTGCCCGCGCGGCCCGGTCGGCGGCGTCGTCGGCCGCCCGGCTCTCGCTGAACACCAGCCGGACCACGGCCCGGGCCAGGACGGTGTTCACCACCACCCAGATGACACTGCGCAGCACGCCGGGCCACTGGAGGTGCGGGTGCAGCGCGGCGCCCACCACATCCGCGCAGCACAGCCACAGCGTCGCCAGCATCGCGGTGCGCAGCGGCTGCATCAGCTGGTACGCCACGGCCGTGAAGTTGACGCACACCATGACCCATGTGCCCCCGTGCGTGGTCTGCGTCTCGGGGATGGTGAACCGCTGGCTCAGACACAGCGCCGTCATGACCGCCAGGTCCAGGGTGAGCAGCGACCTCGGCGGCATCGCCGGGGTGGTGGCCAGCCGCAGGTGGATCCAGCTCCAGGCGAGCACGGCCGCCGCGACCACCGCCGTGGGCGCCATCTCGTCCACCGGCATGGCCACCACGCCCAGCACGGCGCACGCGCTCATCACCCCGGCGCGCCCGTAGAGCGCCAACCGCCGGTTGAACGCGACGTATTCACGCGCCCAGGGCCCTCCCGAGCCGGGAAGGCGCGCCGCGGCCGGCGGGTCGATACCGGGCGACAGTGAGCGAATCGGCATGGGCATCCATCCTCGGCGGCCATCGATGTGGCGGGGCGGTAGAGGTGACCTCACGATCCGTGAGGAAATCGAAGCCTTTTGTCGGTCGAACGACCCTCACACCGGAGGACCCGGCGCGTCTAGGCTGGACCGAGCGCAGTGGCTTGGGGGCGCAGATCGGGATGATCT GGAAGCCCGCCCACGTCGGGCCCCCACAAGCCCTTTGCGAGCAAGAAGTATGCCGCCCATCGGCGCGCTTTCCGCCTCATGCCAACCTTCTCTTGGGTGTATAGCGAAGGGAATGCAGCCTTTTATGTCCGCATCTGCGCCCAATGTCATCCTGCTCGGCGGCCCCACGGAGCTGGCCGATCCGCACACCCGGTACGTCGATGACACCGAGTCGACCCTGAAGCTCCTCATCGGCAACGCGTACGAACACTTCGAGCCCACAGGCCAGTTCATGGAGCACCACGGGCGGCATCTGCGGGTCTTCGAGTGGATCCGCCGGACGTATGTCGCCGAGTAATCAGCCACGCGGCCAGGGCCCGGTCTTCGCCGGCGGGTTCACTCGCAGATGTGCGAGTCGAACCCCCGGCGAGCTCGGGCGGTGGCCTGCAGAACGAGTCCGCCAGGCGCGGTCCGTCAGGTCGAGGCGAGAACCGTGTGCCGGAGCTCCCTGAGTTTCCGGCCGGGTTCAAGCCCCAGTTCCCTGTCGAGAACACGTCTGGCGGACTCGTATATGCCAAGCGCCTCGGACTTCCGGTCGGAGCGGTAGAGGGCCAGCATCAGCTGTCGGTAGAACGTCTCCCGGAGCGGGAACTCGGCGACGAGGGAGTGCAGCCGGCCGACGAGTTCCCGATGGCGGCCGAGTTCGAGATACGCCTCGGCCGACATCTCAGCACATTCAATACGCATCTCGTCCAGCCGCTTAAGAAAACCGGTCACGATCGGTCCCGTCTGGACATTCCCGAACGCCGGGCCCCGCCACAGCGCCAGGGCCTGTTCGAAACACAGCGCCGCCTGCGCGGGCTCCTCGTATCTGACGTACTTCCGCCCGTCGGTGACCAGCTGCTGGAAGGCGTCGAAATCGCATTCGCTCGACGCCCTGCGCAGCATATATCCACCCGGGCGGGTGATGATGGGATTCTGCTGGCCCGAACGGCTCAGGAACTTGCGCAGCCGCGAAATGCAGACGTAGATACTCGCGGTGTCGCGCCGAGGCGGCAGCTCGCCCCAGATCTCTCTGGTGAGCTGTTCCGCCATCACGACCTGATCCGAGCGGATCAGCAGGACGGCGAGAACGGTAGCGAGTTTCTGCGCTCTGAGCGGCGAACCGCCCTTTTCGTCGACTACACGGAGAGGTCCCAAAATCTCGTACCGCACGAATTACCCCCTGTTCTGCTGTTCCTGGATGCGTGACGAGTCGGCGGTGCGCGCGATTCGGCTAGGCGGCTTCGAGTTCCGGGGCGTCGATGAGCACGGCATGGTGCAACCGCTTGAGGGCGGCGGACGGCTCAAGCCCGAGTTCCTCGCTCAGCACGGTCCGCGCCGACTGGTAGACACTCAGGGCGTCGGCCCTGCAGCCCGAACGGTAGAGCGCCAGCATGAGCTGCCGGTAGAACGCCTCGTTCAGCGGAAGCTCCTCTATCCGGGCGTAGAGGTAGCTGACGAACTCACGGTGGCGGCCGAGCTCCAGGCCGGCGTCCACGAACGACTCGACGCATTCCAGCCGGGCCTGCTCGGCCCAGCACACGAAGCCGTTGACGATCGCCCCGTCGCGCAGGTCCTCGAGGACCGGGCCGCGCCAGAGGTCAAGGCCCTCTTCGTAAGCCTCGCAGGCCTCATGGGTGCGCCCGGCCCGCTGTGCGGCCCGCCCCTGGCGCACCAGCCCCTGGAAGACATGGAAGTCCAGCTCGTCCGCGCCGAGACGCAGCACATACCCGCTGCGAGCGGTCACGATGGGGCTCTCGGTGCGGTTGGGGCGCCTGAGGAACTTGCGGAGTTGGGACACATAGACGTGGAGCGCCGCCACGGCCTGCCGCGGCGCCCGGGTGCCCCAGATCTCCTTCACCAGATCTTCGCTGCTCACCACCTCGTCGGCCCGCACGAGTAATGCCGCCAGCAGCACCTCGATCTTGCGGGCACTGATTGAGGTGACCGAAGATCCGTCAACCACGCGCAACGGGCCCAGTATCTCGTACCTCATGATACGCGAACTCCCCCCATCGCCGGCGTCGTGTCGAACCAGGAGTGCGCTCCATTGTGATCGAATTCGGCCAGCATCCGAAAAATTCACCCCGATTTTGGAAGCCTTTATGGTCGCGCTTCAGACCTCAGCGTCGATGCCTACCCACGCCACCGACAGCTTAGCCAGATCGCAGCCACCTCCGCGCATTTCACATGGCTGCAACATCGCCCCCAGTGAGGTTTTCGGTCAGATGGGAGGTGGTCTTCTGGCTACGGAGATCGACGGCGTTGCTCTGCGATCGACGGCTGCGCCGACTCATGGTGCCGAACTGCGCCCGGCATCACGGGCCGCGGTCCTCTCCGAAGGGCGAACAACCAGCAGCAGTGATCGCAGAGAGGGCAACATCAACGCATCCTAACTGTGCGGCGCGCGCCAGTGTGAGGGTCAATAGACCCACAACCAAATCGGCACATAGGGTGATTCGTCGATCGGTGACCGTCACCGTACCCTGCCGTTTCGGTGGCGGCCCCACGCAGGTCACCGCTCTGATCCGAAGCATCAGCGGGTCAAGTTTCGCCCGGCGTATACAAGGAGCTCCCCAGACATTCCCGGTTAGATCAACTTTAGAAAACATTCGGAGAATGAGCGCTGTTCGGGGCATCTCGTAAGCCATAGCCAGCGGTTTCGCGATTCAGGTATGGGGGTGTCATGGCCGGGAGCAGCCCGAGCCACGCGCAGCAGGCGGCGTCTCCCGTCGCGATCGTGGGGCTGGCCTGCCGGCTGCCCGGCGCACCCGATCCCGAGGCGTTCTGGCGCCTGCTGCGCGCGGGCGAGAACGCCGTCGTCCCGGTCCCGGACGACCGCCTGTCCGCCGAGCCCGGTTCCGCGCCGTACTTCGCCGGGCTACTGGAGCACATCGACACCTTCGACGCCGGATTCTTCGGCATCTCGCCGCGCGAGGCCACGGCGATGGATCCGCAGCAGCGGCTGATGCTCGAACTGGCCTGGGAGGCCATGGAGGACGCGGGGCTCGGCCCCGGGAACCTTGCGGGGTGCCGGACCGCGGTGTTCACCGGCGCGATCTGGGACGACTACGCCACGCTCCTCCACCGGCACGGGCCCAGCGACATCGCCATCACCCGGCACACGATGGCGGGCCTGCACCGCGGCATCATCGCCAACCGGATATCCCACCTCCTGGGCCTGCGCGGCCCGAGCCTCACGGTGGACGCGGCGCAGTCCTCCGGGCTCGTCGCCGTACACCTGGCCTGCGAGAGTCTGCGCCGCAGGGAGGCGGATCTGGCGCTCGCGGGCGGGGTGAACCTGATCCTGGCCGAGGAGAGCATGCGGATGGCGAAGGCCCAGTTCCAGGGCCTGTCGCCGGACGGCCGGTGCTACACCTTCGACGCGCGCGCCAACGGCTTCGTACGCGGCGAGGGCGGCGGAATGGTGCTGCTCAAGCCGCTGGCCGCGGCCGTCGACGACGGCGACCCGGTGTACTGCGTGATCGAGGGCAGCGCGGTCAACAACGACGGCGCCACCGACGGCCTGACCCGGC CGAGCGCCGAGGCCCAGACGGATGTGGTGCGGCAGGCGTGGCGGCGGGCCGCGGTGTCGCCCGCCGAACTCCAGTACGTCGAACTGCACGGCACCGGCACACCGGTCGGCGATCCGACCGAGGCCGCGGCCCTCGGCGCCGCGCTCGGCGGGCACCACCAGGACCAGCGTCGGGCCGCCCCCCTGCGGGTCGGCTCGGTCAAGACCAACGTGGGCCACCTGGAGGCCGCCGCCGGGATCGCGGGCCTGCTCAAGACGGCCCTGAGCATCCACCACCGGCGGCTCCCGCCGAGTCTCAACTTCGCCACCCCCAACCCCGCCATCCCGCTGGCCGAGCTCGGGCTGCGCGTCCAGACCGCGTTCGAGCCGTGGCCCGACGAGCGGCGGCGGCTCACGGCCGGGGTCAGCTCCTTCGGCATGGGCGGCACCAACTGCCATGTCGTCCTGGCCGAACCGCCCGCCGCCGCCACACCGTCGGACCGGCCCACGCCGCCCGGGGACGTGCCGAAGCGCACCGTCCCGCCGGTCACGCCGTGGGTCGTCTCCGCGGCGAGCCCGACGGCGCTCGCGGCCCAGGCCGCCGCGCTGTGCGAACACCTGCGCGCACACCCCGGACTCCACCCGGTGGACGTCGGCCACGCGCTCGCCACGACCCGGACGGCGTTCCCCCACCGAGCCGTCGTCCTGGGCACGGGGGTGGCCGAACTGCACGGCACTCTGGACGCGTTGGCGTCCGGGACACAGACGTCGGGGGTGATCCGCGGCCGCGCGGGCGGGGGCCGGGTCGCGTTCCTGTTCAGCGGCCAGGGCAGCCAGCGCCCCGGCATGGGCCGCGAGCTGTACACGGCGTACCCCGTCTTCGCCGACGCGCTGCGTGAGGTGTGCGGGCACCTGGACCCCTTGCTCGACACCGACACCCCGCTGCTGGACCTCATGTTCGCCGAGGCTCCCGCGGACGGTGAGCCGCCGCTGAACCGGACGGCGTACACCCAGCCCGCGCTGTTCGCCGTCGAGGTGGCCCTCTACCGCCTGGTGACGTCCTGGGGCCTGACCCCCGACCACCTGATGGGCCACTCGGTCGGCGAGATCACGGCCGCGCATGTGGCCGGGGTGCTGTCGCTGCCCGACGCCTGCACGCTGGTGGCGGCCCGGGGCCGCCTCATGCAGTCCATCACCACCCCCGGGGCGATGGCCGCCTGGCAGGCCACCGCCGAGGAGGCCGAGCAGGCGCTGGAGGCGCACGGCGGGCGGGTGGGCCTCGCGGCCGTCAACGCACCCGCCTCCGTGGTGATCTCCGGTGACCGCGAGGCCGTGGCGGAGGCGACCGCCGGCTGGCGGGCGCGGGGCCGCAAGGCCACCGTGCTGAAGGTCAGCCACGCCTTCCACTCCCCGCACCTGGACGGCATCCTCGGCGAGCTGCGCGCGGTCGCGGCCGGGCTGACGTTCGCCGCGCCCGCCATCCCCGTCGTCTCCAATGTGACCGGCGGGCCGGCCACCGAGGCCCAGTTGCGCTCCCCCGACTACTGGGCCGACCACGCGCGGCAGGCCGTACGGTTCGACGCCGGTGTGCGGCACCTGTGCGACGCGGGGGTCGACACCTTCCTCGAACTCGGCCCCGACGCCTCGTTGACCGGCATGGCGCGGGAGAGCGCCGCCGCATGGGCCGGGGACGCGCCTCGTCCGACGGCGGTCGCGGTGCAGCGCCGCGGCCGGCCGGAGGCGCACAGCTTCGTGTCGGCGATGGCCCAGGCACACGTACGGGGCGCGGCCGTCGACTGGGCGGCCGCCTTCGCGGGCCACGAGCCCCGGCGGGTACCCCTGCCGACGTACGCCTTCCAGCGCGAACGCCACTGGCCCGACGGGCTGGACGGGCGAGGGGCGGGGCGTACCCGGATCTCACCGGTCGCGCCGCCGGCCGAGGCGTCGCCGCGGGCCGATGCCGCGCCGCGGGCCGATGCTGCGCCGCCCGTCGATCGCGCGGCCACGGCCTCCGTGGGCGAACTGCTGGCGCTCGTCCGTACCCACGCCGCCCTCGTCCTGGGGCACGACAGCCCCGACGGCATCGACCCGGCCCTGACCTTCAAACAGCTCGGCTTCGACTCGCTGGCCGCGACGGAGCTGAGCGAACGCCTCGGCGCGGCCACGGACACCGCACTGCCCGCCACCCTCACCTTCGACCATCCGACGCCGAACGCCGTGGCGGCGTGGCTGCGCGCGGCGCACGAGGAGCAGCCGGCCACCGCCCCGACGGCGGCGGTCGGGGCGGCCACGGCCGATGACCCGGTCGCCGTGGTGGCCGTCAGCTGCCGCTACCCGGGCGGGGCCGACTCCGGCGAGGCGCTGTGGCGCCTGGTGGACGAAGGCGTGGACGCCGTGGGCGAGTTCCCCGGCGACCGGGGCTGGGATCTGGCCGAACTGTTCGGCCGGGCACCGGACGGGAGCGGCGGCAGCGCGACGGGCCGGGGCGGTTTTGTGTACGACGCGGGGGACTTCGACGCGGAGTTCTTCGGGATCAGCCCGCGTGAGGCGCTGGCCATGGATCCGCAGCAGCGCCTTCTGCTCGAACTCTCCTGGGAGTTGCTGGAACGGGCCCAGATCCCGCCGGCCTCGCTGGCCGGCAGCGCGACCGGCGTCTTCGTGGGCGCGACGGCGGCGGACTACGGGCCGAGGCTGCACGAGGCCGCCGCCGAGCTCGACGGACATCTGCTGACCGGTTCCACGCCGAGCGTGGCGTCCGGCCGGGTGGCCTACACCCTCGGCCTGGAGGGACCGGCGCTCACCGTGGACACGGCGTGTTCGTCGTCGCTGGTGGCGATGCACCTGGCCGCGCAGGCGCTGCGGCAGGGCGAATGCGATCTGGCGCTCGCGGGCGGTGTGACGGTGATGGCCACGCCGGGCATGTTCACTTCGTTCTCCCGGCAGCGCGGCCTGGCCCCGGACGGGCGGTGCAAGCCGTTCGCGGCCGCCGCCGACGGCACGGGATGGAGCGAGGGCGCCGGGCTCGTTCTGCTCGAACGCCTGTCCGACGCGCGCCGCAACGGCCACCAGGTGCTCGCCGTGATCCGCGGCTCGGCGGTCAACCAGGACGGCGCGAGCAACGGCCTGTCCGCCCCCAACGGCCCCTCCCAGCAGCGCGTCATCCGCCAGGCCCTGGCCAACGCCCGCCTCGGCCCCGCGGACGTGGACGCGGTCGAGGCCCACGGCACCGGGACGACGCTGGGCGACCCCATCGAGGCCCAGGCCCTCCTGGCCACCTACGGCGGGCACCGCCCCGACGACCGGCCACTGTGGCTGGGGTCCATCAAGTCGAACATCGGCCACACCCAGGCCGCCGCCGGTGCCGCGGGCGTCATCAAGATGGTCATGGCCCTGCGCCACGGCCGCCTCCCGGCCAGCCTCCACATCGACGCCCCCAGCCCGCACATCGACTGGTCCGGCGGCACGGTCAGGCTCCTCACCGAGCCCGTCGACTGGCCCGGGGCCGACCGGCCCCGCCGCGCCGCCGTCTCCTCCTTCGGCATCTCCGGCACC AACGCCCACCTCATCCTCGAACAAGCACCCGACCAGCCCGAACCGGAGCCCGCCACCGCCGCGGGCGGCGTGGTGCCCTGGGTGCTGTCGGCCCGTACCGCCGACGCGCTGCGTGCCCAGGCCGGGCGCCTGGCCGAGTGGGTCACCGCCAAAGCCCCGCCCTCCCCGGCGGACGTGGGGTGGTCGCTGGTCACCACGCGGTCGGCGCACCGCCACCGGGCCGTGGTCAGCGGTACGGACCGGGACGAGCTGCTGTCCGGGCTGCGCGCGGTGGCGGACGGCCCCGCGCCCGCCGCCGTCCCGGCGGGGTCCGCCCCCGGCCCGGTCATGGTGTTCCCCGGGCAGGGGTCGCAGTGGCGTGGCATGGGCGTGGAACTGCTGGACTCCTCCCCCGCCTTCGCGGCCCGCGTCGCCGCGTGCGAGGCCGCGCTCGGCGAGTTCGTCGACTGGTCCCTCACCGCCGTGCTGCGCGGCGCGCCGGGCGCGCCCGAGCCCTCCCGGGTCGATGTACTCCAGCCGTGTCTGTGGGCGGTGATGGTGTCGCTCGCCGCCGTCTGGGAGAGCTTCGGGGTGAAGCCCACCGCGGTCGTCGGACACTCACAGGGCGAGATCGCCGCCGCATGCGTCGCCGGGGGCCTCTCGCTCCGGGACGGCGCCAGGGTCGTGGCGCTGCGCTCCCGGGCCCTGCGCGCCCTCGCCGGCCACGGCACCATGGCCTCGCTCGCGCTGAGCGGCGAGGAGGCCGAGCGCTTCCTCGCGGACCTGGGCGCGGCGGCCGCGCGGGTGACGGTGGCGGTGTTCAACGGGCCGTTCTCCACGGTGGTGTCGGGGCCGACCGACCAGGTCACCGCGGTGGTGGCGGCGTGTGAGGCGGCGGGCCACCGGGCCCGCGCGATCGACGTGGACTACGCCTCGCACGGCCCGCAGGTCGACCGGCTCGCGGACACCATCCGCGCCGACTTGGCGGACCTCGCCCCCGGTGGATCGGGCGCGGTGTTCTACTCCTCCGTCACCGGCGCCCGGCAGGCGACGGAGGAGCTGGACGCCGACTACTGGTTCACCAATCTGCGCCGGCCGGTCCGGTTCGCCCCGGTGATCGACACCCTGCTGGCCGCCGGACACCGCGTGTTCATCGAGGTCAGCCCGCACCCGGTGCTGATCCCCGCGCTGCGGGAGTGCTTCGAGGCGGCCGATGTGGCGGCGGCCACCGTGCCGACGCTGCGCCGGGACCAGGGCGGCCCGGATCAAGTGGCGCGCGCCCTGGGGGACGGCTTCGCCGCGGGGCTCGCGGTCGACTGGAGCCGCTGGTTCGCGGGCGACGAGGGCGGCAGGGGCGACGAGGGCCACCGGCCGCGTACGGTCGAGCTGCCCACCTACCCGTTCCAGCGGCGGCGGTACTGGCTGGCGCCTGACCAGGGCCGCCGCGAGGGGCGTGCGGCCGGAGCCGGAACCCGGCCGGCCGGGCACGCCCTGCTGTCCTCGGCGGTCGAACTGGCCGACGGCGGCCTGGTGCTCAGCGGCCGGCTCCCCGGCGACGCGGCCTGGCTGGGCGCGCACACCGTGGCGGGCGTCCGGTTGGTGCCCGGCGCGGTCCTGGTGGACTGGGCGCTGCTGGCCGCCGACGAGGCGGGCGGGGCGTCGCTGGAGGAACTGCTGCTGCGCGCTCCGCTGGAGCTGCCCGGGGCATCCGGTGCGCCCGGAGCGCCCGGAGCGCCCGGAGGTGTGCTGGCGCAGGTCGCGGTCGGGGCCCCGGACGGGTCGGGCCGCCGTGAGCTGCGGATCTCCTCCCGGCCAGCCGAGGCCACCGCGGGCGGGGGGTGGACGTGTCACGCGGTCGGGTCGCTGGTCCCGGGCGGCCCGCCCGCTCCGGCGGACACCGGGGCGCCGGTCGCCCCCTGGCCGCCGGCGGGCGCCGAGGCGCTGGAGCCGGCCGGGCTGTACGAGCGGGCCGAGCGGCGCGGCTACGGCTACGGGCCCGCGCTGCGGGGAGTGGCGGCGCTGTGGCGGGACGGGACCGAGCTGGTCGCCGACCTGGTGCTGCCCGAGGAGGCCGGTGACGTGGGTGGCGAAGACGGCGGCGGTTCGGCCGGATTCGGTCTGCACCCGGTCCTGCTGGACGCCGCGTTGCAGCCCGCCCTGCTGACCGAGCCGGACGACGCGGGGGGCGAGGGAGAGGGGCCGGAGGCCCGGTTGTGGCTGCCGTTCGCGTGGAGCGGCGTCCGTCTGTGGGCGACCGGGGCGCGCGCCGCGCGGGTCCGGCTGACGCCGCTGGACGGGGACGGCGGGGACGATCGCGAGGTGCGGATCGAGGTGTCCGACCCGGCCGGGGCCCCCGTGCTGAGCGTGGCGTCGGTGGTGCTGCGCCCGCGCACCGTACGGCAGGTGCGCGGGGCGAGCGGGGCCGCGGCGGGTGGTCTCTTCGTCCTCGACTGGACTCCTGTGACGCCTCGGGAGCCGTCCGGCACCGAGGACGACGCCGGGTGTGTGGCCGTGCTGGGCGAGGCGCCGACGGAGCCGGGCGCGGACGGGTGCCGGGACACGTATGCGGACCTCCCGGCGCTGCTGGCCGCCCTGGACGCGGGCGTGCCCGCGCCCTCCGTGGTGGTGTGGCGGCCACCGACCGCCGACCCCGGCGCCGCGCCGGAGGACGCCGCCCTGTTCGCCGTACGGGGCGTGGCGGCGGCGCTGCGCACCTGGATCGGCGAACCGCGGCTGGCGGGCAGCCGTCTCGCGGTGGTCACTGGGTGTGCGGTCGCGGCGGGCGGCGCCGAGGGCGAGCCGGTGGACACGGCCGCGGCGGCTGCCTGGGGCTATGTGCGTGGTGTCCAGGCGGAACACCCCGGCCGCTTCGTCCTCGTCGATGTGGACACGGACACCGACATCGGCACCGACGTCGGCACGGTGGGGGCTCGGGCGGCCGCGCTCGGTGAGCCGACAGTCGCGGTGCGCGGAGACACGCTGCTCGCGCCACGCCTGGCGCGTTTCGCCGCCGCTTCGGGCGGCGTGGCGTTCGACCCGGAGGGCACGATCCTGGTCACGGACGCCGGGGGCCCGCTCGCCGGTGCGGTCGTCGAGCAGCTGGTGCGCGCGGAGGGCGTCCGCCACCTGCTGCTCGTACGGTTCGGCGGCACGGACGACACCGACGACGCGTACGGCACGCAAGACGCCCACGTACGCGTGGTCATGGTCGATCCTGGCGACACCGCCGCGCTCGAACAGGTCGTGGCGCAGGTCGATCCGGCCCATCCGCTGACCGGCGTCATCCATGTCGCGGGGCTGTCCGCCGACATCGAGACACGTGGCGCGGCGCGGGGGTGGGCCGTGGCGGCCGGGGCGGTGCGGGCGCTGCACCATGCCACCGCCGGGCTGCCGTCCGTCCGGTTCGTGACGCTCTCCGACGCCGCCACGGCCTGGGACGGCCCGGTCGCCCCCGAAAGGGCCGCGGCCGGTGCGTTCTGCGAGGCCGTGATGGACGTACGGCGCCGGGCCGGACTGCCGGGCCTCGCGGTGGCGTTCGGGCCCTGGG CCGCAGCCGATGCCGCAGCCGATGCCGACGGCGGCGACGACGACGGCGACGACGGCGGGCGCTGGACGGGGGTCCTCGGCGCGGACCGCGGGTTCGCACTGCTGCGCGCCGCCTGCCGCGCCGACGGACCGGGGCTGGTGGCGGCCGACATCCGTACCCGGGCCCTGACCGCCCACCCGGTCCATGAGCTGCCGGCCGCGCTGCGCGCGCTCGTCGCGGGGTCGGCCGGCCGGGCGCCGGTCCGGCCGGTGGCCGCCGCCGCGCCCGGCCGCGCCACCGACTGGGCGAGCCGGCTGGTGGGCCTCGGGCCGACCGAACGGCGCCGCGCCGTCCTGGAGCTGGTGCGCGATCACGCCGCCGCCGTGCTCGGGCAGCCCGACCCCAAGGCCGTACGGGCCGACGCCTCGTTCAAGGAGCTGGGCTTCGACTCGGTGACCGCCGTCGAGCTGCGCGACCGGCTGGTGGCGGCCGGCGGGCTGCGGCTGCCCGCCGCCGTCGTCTTCCGCCATCCGACGCCGGAGGCGCTGGCCCGCCGGATCGAGCAGCAGCTCGCGCCCGACGGCACGAACAACACCGACGGTGCGAACAACGCCGACGGTGCGGACAACGCCACAAAGAACGCTGGGAACAACAGCAACGGCCCGGCGCTCGACGCGGCGGACAAGCTCGCGTCGGCCACGGCCGACGAAATCCTCGACTTCATCGACAACGAGCTCGGCGTGCTCTCCGAGGCGCGTCCGCGCCCCTCCAACTGAAGGCAGGTGACGACATGGTCAGCGAGGAGAAACTGGTCGAGTACCTGCGCCGGGTCACCACCGAGCTGCACGACGCCAGGACCCGGCTGCGGGAGCTGGAGGAGGGTGAGCAGGAGCCGGTCGCGGTGGTCGGCATGGCCTGCCGTTTCCCCGGCGGGGTGCGGTCCCCCGAAGACCTGCGCCGGCTCGTCCTGTCGGGCGGCGACGCGATCGGTGACTTCCCCACCGACCGGGGCTGGGACCTGGACGGCCTCTTCCACCCGGATCCGGCGCACTTCGGCACCAGCTATGTGAGGCAGGGCGGCTTCCTGTACGACGTCGACCGGTTCGACGCGGGGTTCTTCGGCATCAGCCCGCGCGAGGCGCTGGCGATGGACCCGCAGCAGCGGCTGCTCCTGGAGCTGTCGTGGGAGGCGCTGGAGTCCGCCGGGGTGGTGCCCGGCGCGCTGCGCGCCAGCCGGACCGGTGTGTATGTGGGGGTGTCCAGCGAGGACTACATCTCCGGACTGCCGCGGATCCCGGAGGGCTTCGAGGGCTACGCCACCACCGGCAGCCTGACCAGCGTCATCTCCGGCCGCGTCGCGTACACCTTCGGCTTCGAGGGCCCCGCGGTCACCGTGGACACCGCGTGCTCGTCGTCGATGGTGGCCATCCATCTGGCGGGGCAGGCGCTGCGGCAGGGCGAGTGCTCCCTGGCGCTGGCGGGCGGCGTCACGGTGCTGTCCACGCCGCTGATGTTCACCGAGTTCTGCCGGCAGCGGGCGCTCACACCCGACGCCCGGTGCAAGCCGTTCGCCGCCGCCGCAGACGGCACCGGTTTCTCGGAGGGGGCCGGCCTGCTGCTGCTGGAGCGGCTGTCCGACGCGCAGCGCAACGGCCACGAGGTGCTGGCCGTCCTGCGGGGCTCGGCCATCAACCAGGACGGCGCGAGCAACGGGCTGACCGCGCCCAACGACGTCGCCCAGGAGAACGTGATCCGCGACGCGCTGGCGAGGGCCGGGCTGTCCGGCGCGGACGTGGACATGGTCGAGGCGCACGGGACGGGCACCCGGCTGGGCGACCCCATCGAGGCGGAGGCGCTGATCGCCACGTACGGGGCGGACCGCCCGGCGGAACGGCCGCTGTACCTCGGCTCGATCAAGTCGAACATCGGCCATACGCACGCGGCGGCCGGGGTCGCGGGCGCGATCAACACCGTGATGGCGCTGCGGGACGGCAAGCTGGCCAGGACCCTGCACATCGACGAGCCGACCCGCCACGTGGACTGGAGCTCCGGCACGGTGCGGCTGCTGACGGACCCGCACGACTGGCCGGTGACCGACCGGCCGCGCCGGGCGGCGGTGTCGTCGTTCGGCGTGTCCGGCACCAATGCCCATGTGATCCTCGAACAGGCCCCGGACCAGCCCGAACCGGCCCCGTCCGGCACCGCGGACGGCGGCCGCCACGGCGTGGTGCCCTGGCCCGTCTCGGGGCGTACCGAGGCGGCGCTGCGGGACCAGGCCGCGCGGCTGGGCGCCTTTCTGACGGCGGACGGTGCGGCGAGTGGCGCCGCGGACGTGGGCTGGTCGCTGGCGACGCGCCGCTCGGCGTTCGAGCACCGGGCCGTCGTGGTGGGCCGCGAGCGGTCGGACCTCCTCGCCGCGCTCGACGGTCTCGCGGCCGACGAGCCGGGCCCCGCGGTGGTGCGCGGGGTGGCGGCGGACGTGGGCGCGGGCCCGGTCATGGTGTTCCCCGGGCAGGGGTCGCAGTGGCTCGGCATGGGCGTGGAACTGCTGGACACCTCCCCCGTCTTCGCGGCGCGCCTCGCGGCGTGCGAACGGGCCCTGGCCGCGCATGTCGACTGGTCGCTGACCGACGTGCTGCGCGGCGCGCCGGGGGCGGCCGACATCGGCCGGGTCGATGTGGTGCAGCCGGTGTTGTGGGCGGTGATGGTGTCGCTGGCCGCGGTGTGGCAGGCGCACGGCGTACGGCCGGCCGCCGTGGTCGGCCACTCGCAGGGGGAGATCGCGGCGGCCTGTGTCGCGGGCGCGATGACGCTGGAGGACGGCGCCCGTGTGGTGGCGCTGCGGGCGCGGGCGCTGCGGGCGCTGGCCGGGCAGGGCGCCATGGCCTCGCTGGGCTGCGGTGTCGAGGGGACGGAGCGGCTGACCGCCGCACACGCGCCGGACGTGGCGGTCGCGGCGGTCAACGGCCCGTCGTCCACGGTGGTCTCCGGGCCGCCCGAGCAGGTCGAGAAGCTGGTGGCCGCCGCGCGCGCCGACGGGCTGCGGGCCCGCGCGATCGACGTGGACTACGCCTCGCACGGCCCGCAGGTGGACGGCATCGCGGACGAGCTGGCCGAGGTGCTGGCCGGGGTGTCCGGCGCCTCCTCCGACACCGCCTTCTACTCGACCGTGACCGGCGCCCGTATGGACACCTCCGGTCTCGACGCCGGCTACTGGTTCACCAATCTGCGCCGGCCGGTGCGTTTCGCCGAGGCGGTCCAGGCCCTGCTCGACGCCGACTACCGGGTGTTCATCGAGGTCAGCGCGCATCCCGTGCTGCTGCTCGGCCTGCAGGAGTGCTTCGAGGCGGCAGGCGTCCCGGCCGTGGCGATCGGCACGCTCCGCCGGGACGAGGGCGGCCCCGAGAGGCTGTGCCGGGCGCTGGCCGAGGCGCATGTCGCGGGCGTGGCGGTGGACTGGGCGAGCTGGTACGCCGAGGGGCCCGCGCCCGCGGCGGTGCCGCTTCCGGTGTACGCCTTCCAGCGGGAGCGGTACTGGCTGCCGG CCGGTGGGAGCTCCGGTCCCGGCGATGTCGCTGGCGCAGGGCTCACCGCGGTCGGACACGCGCTGCTCCCGGTGTCCGTGCGCCTGGCCGACGGGAGCCTGGTGCTCAGCGGGCGGCTGCCGGAGGCGGCGCGGGGCGGCTGGCTGGCCGAACACCTGGTCGCGGACCTGCCGTTGCTGCCCGGCGCGGTGCTGGTCGAATGGGTGCTGCGGGCGGCCGACGAGGCCGGCTGCGGCGGTGTCGAGGAGCTGGCGCTCCAGGTCCCGGTGGCGCTTCCCGCATCGGGCGGGCTGGTGATCCAGGTGGTCGTGGACGCCGCCGAGGGCGACGGGCGCCGTCCGGTGCGGGTCTACTCCCGGCCCGAGGAGGACACAGCGGCGCCGGACACCTGGGTCTGCCATGTCTCGGGCACGCTGCTCCCCGGCGCGGCCGGGCCGGTTCCGCCGCCCGGTCTCGGCGGGGCGTGGCCGCCGCCGGGGGCGCGGCCGGCGGCGATCGAGGGGTTCTACGAGCGGGCCGGGGCAGCTGGTTACGGCTACGGCGCGTTCTTCCGGGGCCTCGCCCACGTATGGCACGACGGCGAGGACACGCTGGCGGAGGTGGTGCTGCCCGAGGAGGCGGCGGAGCAGGCGGGTGGCTTCGGTATCCATCCCGCCCTGCTCGACGCGGCGATGCAGCCCGTACTGCTGGCCGGTCAGCTCGCCGCCGCGCCGGGTGTCGACGCGGCGCCCGGGACGGGGACGGTGCTGCTCCCGTTCACCTGGAGCGGGGTGCGGCTGTGGGCGGGTGGCGCCACCCGGCTCAGGGTCCGGCTGTCGCCGCGGCCGGAGGGGCTGCGGGTGCTGCTGGCCGACGCCACGGGCGCCCCCGTGCTGACGGCGGACGCGGTCGCGCTCCGTGAGACGGGCGTCCGGCAGTTGCGCGCGTCGAGCCGGGTGCGGGGGTCGCACGGGCTGTTCGCCGTCGAATGGATGCCGCCGCTGTCCGCCCCGGCGGGCGGGACGGCGTCCGGGACGGCCCCCGCGTCGCTCGCGGTGCTGGGCGACGAGGCCCCGGACCTGGCCGGCGCCGACCGTTATCCCGACCTCGACGCGCTGTTCCGCGCCGTGGACGGCGGAGCCACCGCACCGGATGTCGTCGTCGCCCCGGTGCGTACGGACCCGGCGGAGGCGGACACCGGCTTGGCGGCCGCGCGGCGGACGCTGGCGCTGGCCCAGGAGTGGCTGGCCGGCAGCGGGGTCGAGGGGGCGCGGCTCGCCGTGGTCACCCGGTCGGCGGTGCGGACCGGGGACGGCGACCCGGAGCGGGTGGACCCCGCGGCGGCCGCGGTGTGGGGGCTGGTGCGCAGCGCCCAGACGGAACACCCGGGGCGTTTCCTCCTGATCGACATCGACACCGACACCGACACCGACGCCGACGCCGACACCGGCCTGGAGGCATTGCGGGACGCGTTGCGGGAGGCGGTGCGTACGGACGAGCCGCAGCTCGCGCTGCGTGGCGGGCGCGCGTTGGTGCCCCGGATGGCCCGGGTGGACGCCGAGCGGGAGCTGGTGGCCCCGGCGGGTGAGCGGGCGTGGCGCGTGGTGGCCGGCAAGACCGGCCCCGACGACCTCACGGCGGTGCCCAGCCCCCGGGCCTCGGCTCCCCTCGCGCCCGGCCAGGTGCGTATCGCCGTACGGGCCGCGGGGCTCAACTTCCGCGATGCGCTGATCGCCCTGGACATGTACCCGGACGCGGCGGCGTCGATCGGCAGCGAGGGCGCCGGTGTCGTGCTGGAGGTGTCCGAGGGGGTGGCCGGGATCGCCGTCGGCGACCGCGTGATGGGCCTGTTCGACGACGCGTTCGGCCCGGTGGCGGTGGCGGACGCCCGGATGGTCGCCCCGGTGCCGGACGGCTGGAGTTTCCGGGAGGCCGCGGCGGCCCCGGTGGCGTTCCTGACCGCGTGGTACGGCCTGGTGGAGCTGGGCGGGCTGCGCTCCGGCGAGACCGTGGTGGTCCACGGTGCGGCCGGCGGCGTGGGCATGGCCGCCGTCCAGGTCGCCCGGCACCTGGGCGCGGAGGTGTTCGCGACGGCGAGCCCGGCGAAGCACCCGGTGCTGGAGGGCATGGGTGTGGACGCCGCCCACCGGGCGTCCTCGCGGGATCTGGGCTTCGAGGCGGCGTTCTCGTCGGCGACCGGCGGCCGGGGCGTCGACGTCGTCCTCAACAGCCTGGCCGGGGAGTTCACGGACGCCTCGCTGAGGCTTCTCGCACCCGGCGGGCGGCTGATCGAGATGGGCAAGACGGACGTACGCGACCCCGATCAGGTCGCCCACGAACACTCGGTCGCCTACCGGGCGTTCGACCTGATCGCGGACGCCGGTCCGGAGCGCATCGGGCGGCTGCTGGCGGCCCTGGGCGAGCGGTTCGCCGACGGCGCGTTCACGCCCCTGCCCGTCACCGGGTGGCGCCTCGGCCAGGCCAGGCAGGCGCTGCGGCGGCTCAGCCAGGCCCGGCACACCGGGAAACTGGTGCTTGACGTGGATCCGGCGCCGGACCCGGACGGCACGGTGCTGATCACGGGCGGCACCGGCACCCTCGGCGGCCTGATCGCCGAACACCTGGTGCGCTCGCACGGGGTACGGCATCTGCTGCTGCTCAGCCGGCGCGGCCTGAGCGCCCCGGGCGCCGAGGAGCTGACGGCCCGCCTCACCGAACTGGGCGCGCGGGTGCGGGTGGCCGCGGTGGACGTCGGGGACGCCACCGCCCTGGACGAGGCGGTCGCGGGCGTCGACCCGGCGCATCCGCTCACGGGCGTCGTCCACGCCGCGGGCGTGGTGGCCGACGCGATGCTGCCCTCGCAGGACGAGGAGCGCCTGGCGGAGGTCTGGTCGGCCAAGGCCGCGGCGGCGGACCGTCTGCACCGCGCGACCGCCGGGCTGCCGCTGGCCATGTTCGTGCTGTTCTCGTCGTTCGCGTCCACCCTGGGCACCCCGGGGCAGGCCAACTACGCGGCCGCCAACGCGTACTGCGACGCCCTGGTCGAGCGGCGGCACGCCGAGGGGCTGCCGGGCGTGTCGGTGAGCTGGGGTCTGTGGTCCGCCGCGAGCGGGCTCACCGGAGGGCTGACCGAGGCCGATGTGGCGCGCATCGCCCGGCAGGGCATCGTCCCGAACAGCACCGAACAGGGACAGGACCTGTTCGACGCGGCGCTCGGGCACGGGCGGCCGGCGCTGCTCGCGTGGAACCTGGACACCCGGGCGCTGGCCGCGCAGCCCGTCGCGGCCCTGCCCGCGCCGCTGCGCGCCCTGGCGGCGGACGCCCAGGCGGGCGCCCGCCTGGCCGGCCCGGCGGCGCGGCCGACCGCGGCGGCGGCCGAGGAGCCCGGGGACTGGGCGGCCCGGCTGGGCGCCCTCGCCCCGGCCGAACAGCGGCGCCTGCTCACCGACCTGGTGCGCAGGCACGCCGCCACCGTGCTCGGCCACGCCGACCCCGAGGCCGTACCGGCCGACGCGGCGTTCAAGGAGCTCGGGTTCGACTCGCT GACCGCCGTCGAGCTGCGCAACCGCGTCACCGCCGCCACCGGGCTGCGGCTGCCCGCCACGGTCATCTTCGACTACCCGGAGCCGGGGGCGCTGGCCGAGCACCTGCGCGCCCAACTCGCCCCCGAGGCGGGGGCGACGACGGCGCCGGACCTCTACGCGCCCGTACTCAGCCGGCTGACGGGCCTGGAGGAGACCCTGACGGCCCTCACCGCGTCCGGGGTGAACGGCGGTACGAGCACAGCGGCCGACCCCGGCGCGGTGACGGCGCGTCTGGAGTCGCTGCTGGCCACGTGGAAGGCGGCGCACGCCCCGCACCGGAACGGCGGCACCGCGGCCGAACGGCTCGAAGCCGCGACGACCGACCAGGTCCTGGACTTCATCGACAAGGAACTCGGAGTGCAATGAGCCCGGTCTGCCAGGAACGCCGAGAATCCCGAGAAACCTCGAAGGCGAAGTGACGCGCCGTGACCACTGAGACCAACGAAGAGCGGCTGGTCGACTATCTCAAGCGGGTCGCCGCCGATCTGCACGACACCCGGGCGCGGCTGCGCGAGGTGGAGGACGGGCAGCGGGAGCCGGTGGCCATCGTGGCGATGGCCTGCCGCTACCCCGGCGACGTCGCCTCCCCCGAGGCCCTGTGGGACCTCGTCGCCGCGCGCCGGCACGCGATGACCGCGTTTCCGGACAACCGCGGCTGGGACCTGGAGCGGCTGTTCCACCCGGACCCCGACCACCCGGGCACCAGCTACGCCCGCGAGGGCGGATTCCTGCACGACGCCGACCGGTTCGACCCGGAGTTCTTCGGCATCAGCCCCCGCGAGGCGGCGGCGGTCGACCCGCAGCAGCGGCTGCTGCTCGAAGTGGCCTGGGAGGCGCTGGAGCGGGCGGGCATCGCGCCCGGGTCGCTCAAGGGCGCGCCCGTCGGCGTGTACGCGGGCACCGCCCTGCCCGGCTTCGGCACCCCGCACATCGACCGGGCCGCGGAGGGTTATCTGGTCACCGGCAACGCGCCGAGCGTGCTGTCCGGCCGGGTGGCGTACACCCTGGGTCTTGAGGGGCCCGCGGTCACGGTGGACACGGCGTGTTCGTCGTCGCTGGTGGCCATGCACCTGGCCGCTCAGGCGCTGCGGCAGGGCGAGTGCGAGCTGGCGCTGGCGGGCGGCGTGACGGTCATGACCACGCCTTACGTGTTCACGGAGTTCGCGCGGCAGCGCGGCCTGGCGGCCGACAGCCGGTGCAAGGCCTTCTCCGAGGCGGCCGACGGCACGGCCTTCGCCGAGGGTGCGGGGCTGCTGGTGCTGGAGCGGCTGTCGGACGCCCAGCGCAACGGGCACCCGGTGCTGGCCGTGGTGCGCGGTTCGGCGATCAACCAGGACGGCGCGAGCAACGGGCTGACCGCGCCCAACGGCCTGTCGCAGCAGCGCGTCATCCGGCAGGCGCTGGCAAGCGCCCGGCTCTCCCCGGCGGATGTGGACGCCGTCGAGGCACACGGCACGGGCACCACGCTCGGCGACCCGATCGAGGCCCAGGCGCTGCTGGCCACCTACGGTCAGGAGCGGCCCGAGGGGCGGCCGCTGTGGCTGGGCGCGATCAAGCCGAACCTCGGGCACACGCAGGGCGCGGCGGGCGTCGCCGGCGTGATCAAGATGGTGATGGCGCTGCGGAACGCCTCGCTGCCCGCCCTGCTGCACGCCGACCGGCCCACGCCCGTGGTCGACTGGGACGGCGGCGCGGTGCGGTTGCTGGCCGAGCCGGTCTCGTGGCCGGCCGGGGACCGGCGGCGCCGGGCCGGTGTCTCGTCCTTCGGCATCAGCGGCACCAACGCGCATCTGATCCTGGAGGAGGCCCCGCCACGGCCGGACACCGAGCTGCCCGAGAACGGGCCTCAGCGGCCGGAGGGCACCGCCGTGCCGTGGGTGGTGTCCGCGCGCGGCGCGGCGTCGCTGCGGACGCAGGCCGCGGCGCTCGCCGAGCACGTGGCGGCGCACCCGGACGTCCCGGCCCGCGACATCGGCTGGTCGCTGGCCACCACGCGCTCGCCGCTCGACCACCGCGCGGTCGTGCTGGGCGCGGACCGCGAGGAGTTGTCGGCCCGGCTCGCCGACCTCGCCGAAGGGCGGACACACCCCGATGTGACGCGGGCGTCCGCTCCGGCGCGGCTCGGTGGCAGCGCGTTCCTCTTCACCGGGCAGGGCAGCCAGCGCCCGGGCATGGGCGCCCAGCTGTACCGGGCCCACCCGGTGTTCGCCGCCGCGTTCGACGAGGCGTGTGCGGCCCTGGACCCCCAGCTTGACCCCCATCTGGGGCGGTCGCTGCGGGAGTTGGTGTTCGCACCGGCCGACGGAGACGGGGCCGGGGCGGGCGCGCTGGACGCGACGGAGGTGACCCAGGCCGCGCTGTTCGCGGTCGAGGTCGCCCTGTACCGGCTGGTCGAGTCGTTCGGTGTGGTCCCCGGCTATCTGGCGGGACACTCGGTCGGCGAACTGGTCGCCGCGCATGTCGCCGGGGTGCTGTCGCTGCCGGACGCGGCGCGGCTGGTGGCTGCCCGGGGGCGGCTGATGCAGGCGCTGCCCGAGGGCGGCGCGATGGTCGCGCTGGAGGCCGCCGAGGAGGAGGTGGCGCCGCTGCTCGCCGGACGGGCGGACCGGGTGGCGCTGGCGGCCGTCAACGCGCCGACGTCGGTGGTGGTCTCGGGCGACGAGGAGGTGGTCGAGGAGATCGCCCGTACGATCCGGGAGCGGGGACAGCGCACCAGGCGGCTGCGGGTCGGCCACGCCTTCCACTCGCCGCGGATCGACCCGATGCTGGAGGAGTTCCGGCGGGTCGCCGCCTCGCTCACGTACGCCCAGCCGCGGATCGCCGTCGTCTCGAACGTCACCGGCGCCCTGGCCGGGGCCGAGCAGCTGTGCGACCCGGACTACTGGGTGCGGCACGCGCGGCAGCCGGTGCGGTTCCGCGACGGCGTCGCCGCGCTGCGCGCGGAGGGCGTCACCCGCTTCCTGGAGCTGGGCCCGGACGCCGTGCTCACCACCATGGCGCGTGACTGCCTGACCGCGGAGGGGGCCCCCGGGGCAGCCGCCGGGGAATCGGCTCAGGGTGCGGGTCCGGGGTCCGGTCCCGGCGCGCCGGTGTTCGCCACGGTGCTGCGCAAGGGCCGCGACGACTCCCGTACGCTGCTGACCGCGCTCGCCCGGCTCCATGTGGACGGCGAGAGCGTCGACTTCTCCGCCGCCTTCCCGCCCGGCGCCCGCGCCACCGACCTGCCCACCTACCGCTTCGACCGGCGCCGCTACTGGCGCGACGCCCCGCAGGCGGAGGCGGACGTACGGGCCGCGGGGCTCGAGGCGAGCGACCACCCGCTGCTGGGCGCAGCCCTCGAACCGGCGGACGGCGGGCTGCTGCTGACCGGCCGGCTGTCGCTGCGGAGCCAGCCCTGGCTGGCCGACCACGCCATCGTGGACGCCGTGCCACTGCCCGGCACGCTCTTCGTGGAGCTGGCCCTCCAGGCCGGCGAGCGGA CCGGCTGCGACCGGATCGACGATCTCACCCTGGAGGCGCCGCTGCTGCTGCCTCCGGTGGGTGCGGTGGACCTCCAGGTGGCCGTCGGGGCCGCCGACGCGGCCGGCCGCCGGGCCGTCACCGTCTATTCGCGCCCGTCCGGCGACGGCTCGGCGGACCCTGGTGTCCCGGAGGAGTCGGGCGACGGCCCGTACAGCCCGTGGCGCCGGCATGCCACCGCCACGCTCGGCACCGCGCCGGCCGGCGCGGGGACCGGCGTCACCGAGCCCGCGGCGGAGCCGGCCCAGTGGCCGCCCGCCGGGGCGGAGGCCATCGACGTGGCCGGGCTGTACGAGCGGCTGGCCGCCGAGGGCTACCGGTACGGGCCCGCGTTCACCGGGCTGCGCGCCGCGTGGCGGGTCGGCGAGGAGATGTTCGCCGAGGTGAGCCTCGCCCCGGACCGGCGGGGCGACGGCGCGGCGTACGCCGTCCACCCCGCGCTCCTGGACGCCGCGCTGCATCCGATCGGCGCGCTGTTCACCGGCGAGGACCAGGCGGGCGGCGCACCGGGCACGGTCCGGCTGCCGTTCTCCTTCGGCGGCGTACGGCTGCTCGCGCGCGGCGCGTCCAGGCTGCGGGTCCGTATCACGCCGACCGGGCCGGACACCGTGACCATGCGGCTGTCCGACGACGCCGGTGCCGAGGTCGTGGCCGTCGACTCGCTCACGCTGCGCGCCGTTTCCGCGCGGCGGTGGCGGTCCGGCGCGATCCCGGCGGACAGGCCGCTGTACCGGCTGGACTGGGAGGCCTTCGCGCCGCCCACCCCCGCGGTACCGGCGCCGGACCGCTGGGCCGTGGTCGCCACGGACGCCGTGAACGGGCCCGACAGCGCTGGCCGTACGGGCGGGACGTTCGCCACCGACGCCGCCACGGCCTCGCTGCCCGGGGAGCACGTCACGCGCCACCCGGACCTGGCCGCGCTGAGCGCGTCGGTCGCCGCCGGCGCCCCGGCCCCCGACCTGGCCGTCCTGGCCTGCCTCGGCGCCCCGTACGACACGTCGGGCGGCGACGAACCGCCCTCCCGGGTACGCGCCGCCACCCGCCACGTCCTCGCGCGGCTGCGGGAGTGGCTGGCGGACGACGCCCTCGGCGCCTGCCGGCTGGTGGTCCTCACCGCCGGAGCCGTGGCCGCCGGCCCGGACGACGCGCCGGCCGACCTGGCGGGCGCGGCGGTCCTGGGCCTGCTGCGCGCCGCCCAGGCCGAACACCCGGGGCGGCTCGTGCTGGTGGACACCGACGACGCCCCGGCGTCCCGGGACGCGCTGGCGGCGGCGGTGGCCGCCGCCGTGGCCGCCGGCGAGGGGCAGCTGGCGCTGCGCGAGGGGCGCGCGCTGGTGCCCCGGCTGGTGCCGGCGCACCCCGACCCCGACGCGGAGCCGGCGGCGCTCGACCCCGACGGCACCGTGCTGGTGACCGGCGGCACCGGCTCGCTCGGCCGGCTGCTCGCCCGCCACCTCGTCGAGGCGCATGGCGCCCGGCATCTGCTGCTGGTGAGCCGGCGCGGGCCGGCCGCCGAGGGCATCGAGGCGTTCGCGGCCGGGCTCGCGGCCGACGTACGGATCGTCAGCTGCGACACCTCCGACCCCGAGGCGCTGGCCGCCCTGCTCGCCACCGTCCCCGAGGAGCACCCGCTGACCGCGGTCGTGCACACCGCGGGCGTACTCGACGACGGCGTGGTCACCTCGCTGACGCCCGAACAGCTCGACGCCGTGCTGGCGCCGAAGGTGGACGCGGCCTGGCATCTGCACCGGCTGACCCGGGGCGCGGACCTCGCCTCCTTCGTCCTGTTCTCGTCCGCCGCCTCCGTGCTGGGCAGCGGCGGACAGGGCAACTACGGCGCGGCGAACGCGTTCCTCAACGCCCTCGCCGAGCACATCCGCGCCGCGGGCGGCCCGGCCACCTCACTGGCCTGGGGCCTGTGGGCGGTGGACGAGGGGATGACGGAACACCTCGCCACCGCGGATCTGGCCCGGATGGCGCGCTCGGGCACGGCCGCGATGTCCGGGGATGCGGGCCTGGCCCGGTTCGACGCGGCGCTCACCTCCGCGCCGCCCGTGCTGATCCCGGCCAGGTTCGACCTCGCCGTCCTGCGCGAGCAGGCGGCCGCGGGCACGCTGCCCCCGCTGCTGCGCCGCCTGGTGCGCCTGCCCGTCCGTACGGCCGCGGCTACCGAGGCGTCACCCTCCTGGGCCGGGCGGCTCGCCGGGCTGCCGGAGAGCGAACAGGACCGGGTGATCGGGGAGCTGATACGCGACCGGATCGCCGCCGTCCTGGCGCACCCCGACCCGGAAACCCTCGAACTGGGCCGGACGTTCTCGCAGCTCGGTCTCGACTCGCTGACCGCGCTGGAGCTGCGCAACGCGATCCATGAGGCCACGGGTGTCCGCCTCCCGGCCACGGCCGTCTTCGACTACCCGACCCCCGAGACCCTGGTGAGCCATCTGCGCACCGAACTGCTCGGCGCCACCGCGGCCACCGCGCCGCTGCCGCCGGGGGCCGGAGCGCCGGCCCGGTCGGAGAGCGCGGAGGACCCCGTCGTGATCGTCGGCATGGCCTGCCACTACCCGGGCGATGTGCACTCCCCGGACGAGCTGTGGCGGCTGGTGGCCGACGGGGTGGACGCGATCGGGCCGTTCCCCGAGGACCGCGGCTGGGACGTGGCCGGGCTGTACGACCCCGACCCCGAGCGCACCGGCAAGAGCTACGCCAGGGAGGGCGGCTTCCTGCACCAGGCGGCCCTGTTCGACGCGGAGTTCTTCGGGATCAGCCCCCGGGAGGCGCTGGCCACCGACCCGCAGCAGCGGCTGCTGCTGGAGACGGCGTGGCAGGCGTTCGAGCACGCCCGGATCGATCCGGCGGCGCTGCGCGGCAGCCGGACGGCGGTGGTCACCGGCATCATGTACGACGACTACGGCGCGCGGTTCCTCGGCCGGATCCCGGAGGGGTACGAGGGCCAGATCATGACCGGTTCCACGCCGAGCGTGGCGTCCGGCCGGGTGGCCTACACCTTCGGCCTGGAGGGGCCGACGCTGACCGTGGACACGGCGTGTTCGTCGTCGCTGGTGGCGATGCACCTGGCCGCGCAGGCGCTGCGGCAGGGCGAATGCGAGCTGGCGCTCGCGGGCGGTGTGACGGTGATGGCCACGCCGAACACGTTCGTCGAGTTCTCCCGCCAGCGCGGCCTGGCGCCGGACGGGCGGTGCAAGCCGTTCGCGGCCGCCGCCGACGGCACGGGATGGAGCGAGGGCGCCGGGCTGCTGGTGCTCGAGCGGCTGTCGGACGCGCGCCGCAACGGGCACCGGGTGCTCGCGATCCTGCGGGGTTCGGCGGTCAACCAGGACGGCGCGAGCAACGGCCTGACCGCGCCCAACGGCCCCTCCCAGCAGCGGGTGATCGGCCAGGCCCTGGCCGCGGCGGGGGTGGATCCCGCCGGGGTCGATGTGGTGGAGGCGCACGGCACGGGTACGACGCTGGGCGACCCCATCGAGG CCCAGGCCCTGCTGGCCGCCTACGGCCGGCACCGCCCGGACGACCGGCCGCTGTGGCTGGGCTCTGTGAAATCGAACATCGGGCACACCCAGGCCGCCGCCGGTGCGGCGGGCGTCATCAAGATGGTCATGGCCCTGCGCCACGGCCGGCTGCCCGCCACCTTGCACGTCGACGAGCCCAGCCCGCACGTGGACTGGGCGGGCGGAAACGTCCGGCTGCTCACCGAGGCCACCGACTGGCCCGAGACCGGCCGGCCCCGCCGCGCCGCGGTCTCCTCCTTCGGTATCTCCGGCACCAACGCCCATCTCATCCTGGAACAGGCGCCCGACCAGCCCGGACAACCACGGCCGGAGCAGCCGGAGTCCGCCGCCGCGGGCGGCATCGTGCCCTTCGTGCTCTCCGCCCGCACCGCCGAGGCGCTGCGCGCACAGGCCGCGAACCTGGCGGGGTGGCTCCCCTCGGCCGAGGTGGCGGAGGTGGGCTGGTCGCTGGCGACCACCCGCTCGGCCTTCGAGCACCGGGCGGTGGTCGTGGGCGAGGACCGGGACGCGCTGCTGGCCGGTCTGGAGAAGCTGGCCGCGGACGAGCCCGATCCGGCGGTGGTGGCGGGAGCGGCGACCGCCGCGGCCGCGGGCCCGGTGTTCGTCTTCCCGGGCCAGGGGTCGCAGTGGCGCGGCATGGGCGTGGAACTGCTGGACACCTCGCCGGTGTTCGCGGCCCGGATCGCGGAGTGCGAGCGGGCCCTGGCGCCGTACGTGGACTGGTCGCTCACCGCCGTACTGCGCTCCACCGAGGCCGACACCGGCACCGGTACCGGCACCGGCACCGAAGCCGCCGACCTCCAGCGCGTCGATGTCGTGCAGCCCACCCTCTGGGCGGTGATGGTGTCCCTGGCCGCCCTGTGGCAGCACCAGGGCATCACCCCGGCCGCCGTGATCGGCCACTCCCAGGGCGAGATCGCCGCCGCCTGTGTGGCCGGGGCCCTCACCCTCGACGACGCCGCCAAGGTGGTGGCCCTGCGCAGCCAGGCGCTGCGCGCCCTGGCCGGCCACGGCACCATGGCCTCGCTGACCCTCGGCGCCGACGACACCGCCCAGCTCCTGGACGAGCTGGGCGACCAGGCCGACGACGTCACCCTGGCCGCCGCCAACGGGCCCGCCACCACCGTCGTCTCCGGCGCGGTGGAGCAGATCGCCACCGTCCTGGCGGCCGCCGAGGCGCGCGGCGCGCGGACCCGCACCATCGATGTGGACTACGCCTCGCACGGGCCCCACGTCGACCGCATCCGCGAGGACATCGTCTCCGTCCTGGGCGACCTCGCGCCCACCGCCTCCGACATCGCCTTCTACTCCACGGTGACCGCCGAACGCCTGGACACCGCAGGGCTCGACGCCGACTACTGGTTCACCAACCTCCGCCGGCCGGTCCGCTTCGCCGACACCCTCACCGCCCTCCTCGCCCATGGACACCGCCACTTCATCGAGGTCAGCCCGCATCCCGTGCTGATCCCCGGGATGCAGGACGGCTTCGAGGCGGCCGGCGCCCCCGCCACCGCCGTCCCCACCCTCCGCCGCGACCAGGGCGGGCCCCACCAGCTCGCCCAGGCCGTGGCCCGCGCGTACACCACCGGTCTGGCCATCGACTGGGCGCCGTGGTACCCGGCCGGGCCGCGCACCACCGATCTGCCCACCTACCCCTTCCAGCGCCGCCGCTACTGGCTCGGCATGGACGGCGGACCGGGCGATCTGCGCGGCGCCGGACTCACCTCGCTGGCCCACGCCCAGCTCGGCGCCGCGGTCGAACTGGCCGACGGGGGGCTGGTGATGACCGGCCGGCTGCCCGCCGCCGGGAACGGCGGCTGGCTCGCCGACCACGTCGTCGCCGACACCCCCCTGGTGCCGGGCACGGCCCTGGTGGAGTGGGTGCTGCGGGCGGCCGACGAGGCGGGGTGCGGCGGGATCGAGGAGCTGGCGCTCCATGTTCCGATGGCGCTGCCCGCGTCCGGTGGGCTGCGGGTCCAGGTCGTGGCGTACGCGCCCGACGGGGACGGCCGCCGCGAGGTGCGGGTGCACTCCCGCCCCGAAACGGAGGACGGCTCGTCGCCCTGGACCTGCCACGCGACCGGACACCTCTCCCCGACCGCCCCGGGCGCCGCGGACCCGGCGCCGGCGGGGGTGTGGCCGCCGCGGGACGCCGAGCCGCTGGACGTGGCCGGCTTCTACGGCCGCGCCGAGGCGATCGGCTACGGCTACGGCCCGGCCTTCCGGGGCCTGACCGCCGCCTGGCGGCAGGGCGCCGACCTGCTCGCCGAGGTGGTGCTGCCGGAGGCGGCCCACGAGGGGGCCGACGGGTTCTCCCTGCACCCCGCGCTGCTCGACGCGGCCCTGCACCCTCTGGCCCTGGACGGCCGGGGTGAGGACGGGCGGATGCGGCTGCCGTTCGCGTGGAGCGGTGTGTCGCTGTGGGCCACCGGGGCACGCGCGGCCCGGGTCCGGATGTCCCCGCTGGAGCACGGCTTCCGGCTGGTGGTGGCCGATACGGCGGGCCAGCCGGTGCTCAGCGCCGAGTCCGTGGTCGTACGCCCGACGAGCGCGCGGCAGTTGCGCGAGGCGGGCGCGCGGCGGGTGGACGGGCTGTACGCGGTGGCGTGGGTGCCCCTGCCGACACCGTCGGACACCACGGCGCAGGTGGAGGCGGGGGCCGCCGAGGGGTGGGCGCTGCTGGACGGCGGCCCGCTCCCGTTCGACCCGTCAGAGGCCGGATCGCCGCCCCGTCACGCCGACATCGACGCCCTGCTCGCCGCCCCCGCCAGGCCTCACACGGTGCTCGTCGGCGTTCCGGCCGCCGAGGGCGACCGGGAATCGGCCGACGGCGCGTTGGCCGTGACCACCGGTGTGCTGACGCTGGCGCGTCGGTGGCTGGACACGCCGGAGCCGGCCGACGCCCGGCTGGTGCTGGTCACCCGGGGCGCGGTGGCGGCTGCGGAAACCGACAACGGCCCCGACCCGGCGGCGGCCGCCGTGTGGGGGCTGCTGCGCAGCGCACAGGCGGAGAACCCGGGCCGTTTCCTGCTGTGCGACATCGACGACGGCGCCGGGCCCGACGATGTGCTCGGCGCCGTCACCCGGGCGGTGGCCCTGGACGAGCCACAGGTGGCGGTGCGCGGTGGCCGCGTGCTGGCGCCCCGGCTGGAGCGGGCGGGCGCACCGGAGCTGATGCCGCCGCCGGACGAACCGGCATGGCGGCTGGCGGCCGGTGACACCGGCACCATCGACAGCGTTTCGGTCGTGGCCTGCCCGGAGGTGCTGGAGCCGCTGGCGCCCGGCCAGGTGCGGATCGGGGTGCGGGCCGCGGGCATCAACTTCCGCGATGTGCTGATCGTGCTCGGGATGTATCCGGACGAGGGGGTGTTCCGGGGCAGCGAGGGCGCGGGTGTGGTGCTGGAGGTGGCCGGGGACGTGACGTCGGTGGCCGTCGGCGACCACGTCTTCGGGCTGTTCGAGGGCGCCTTCGGGCCCGTGGCCGTCGCC GACGCGCGCGCGGTCGTGCCCATCCCGCCGGGCTGGACGGACCAGCAGGCCGCGGCCGTCCCGACGACGTTCCTGACCGCCTGGTACGGGCTGGTGGACCTCGCGGGCCTCAGGGCGGGCGAGTCGGTGCTCATCCATGCCGCCACGGGCGGCGTGGGCACGGCGGCCGTGCAGATCGCCCGCCACCTGGGCGCCGAGGTGTACGCGACGGCCAGCCCCGGCAAACACGCCGTACTGGAGGCCATGGGCATCGACGAGGCCCACCGCGCCTCCTCCCGCGACCTCGACTTCGAGGAAGCCTTCCGCACCGCCACCGGCGGCCGCGGCGTCGACGTGGTCCTCAACAGCCTCGCCGGCGAGTACACCGACGCGTCCTTGCGCCTGCTGTCCGAAGGTGGGCGCTTCGTCGAAATGGGCAAGACCGACAAGCGCGACGCCGAGCAGATCGCCGAGGCGTACCCCGGTGTCCGCTACCGCTTCTACGACCTGGTGCCGGACGCCGGTTTGGACCGGGTCGCGGAGATGCTGGACACGCTCGCCGGCCACTTCGCGCGGGGCGTCCTGGCGCCGCCGCCGGTGCGGGCGTGGCCGCTGGCCGAGGCCCGCCAGGCGCTGCGCCACATGAGCCAGGCCAAGCACACCGGCAAATACGTGCTGGACATTCCCCGCACGCTCGACCCCGACGGCACCGTCCTGATCACCGGCGGCACCGGCACCCTCGGCGCCCTGGTCGCCGAACACCTCGTCACCACCCACCACATCACCCACCTCCACCTGCTCAGCCGCCGGGGCCCGGACGCTCCCGGCGCCGCCGATCTGACGGCCCGGCTCACCGAGCTGGGCGCCACCGTCCGTATCACCGCCACCGACACCACCGACCCCCAGGCCCTGCGCCAGGCCCTCGACACCGTCGAGCCCGACCACCCGCTCACCGGTGTCATTCATGCCGCGGGGGCCCTGGACGACGCGGTGCTGACCGCGCAGACCCCGGAGCGGCTGGCGTCGGTCTGGGCGGCGAAGGCGACGGCCGCGGCCAATCTCCACCGGGCCACCCAGGGGCTCCCCCTGGCCATGTTCGTGATCTTCTCCTCGGCCGCCGGCACCCTCGGCACCCCGGGGCAGGCCAACTACGCCGCCGCCAACGCCTACTGCGACGCCCTCGCCACCCGGCGACACCGCGCGGGACTGCCCGCCACCTCCATCGCCTGGGGACTGTGGGCCGCCACCAGCGAGATGACCGGCCATCTGGCGGAGGGCGACCTGGCCCGGATGAGCCGCACCGGCTTCACCCCGCTGTCCACCCCCAAGGCGCTGGCGCTGTTCGACGCCGCCCGGCGCCAGGGCGGCGCGACCCCGCTGGCGCTCGACCTCGATCCGCGGACGCTCGGCGCCCAGCCGTCCGACGCGGTGCCCGCCGTGCTGCGTACCGTCGCCGCCGCGGGAGCACCGGTCAGGCGTACCGCAGCCGTCGCAGGGTCCACCGACTGGGCCGGCCGCCTGGCCGCGCTCTCCGACGCCGAGCGCCACCGCGAGCTGGTCAACCTGGTGCGGACGCACGCGGCGACGGTGCTCGGCCACAGCGACCCCGCGGCGCTCCGCGCGGACACCTCCTTCAAGGAGCTGGGGTTCGACTCGCTGACCGCGGTGGAGCTGCGCAACCGGCTCTCGGCCGCGACCGGGCTGCGGCTGCCCGCCGCGCTCGTCTTCGACTATCCCGACGCGGAGACCATGGCCCGCTTCCTGGACCAGAAGCTCGCCCCCGCGGACCGGACGGATGCGGCCGTCGACCGACTCGCGCCCGTCCTGAACGACTTGGCCCGGCTGGAGTCCACTTTGGGCAGCCACGACGTGGACGGGAAGGCGCGCGAGACGATCGCGGGCCGGCTCCACGCCCTGCTGTCCAGGCTGGAGGGGTCCACGGCCAGTGCGGCGGACATCGACGGCGAGGCGCTGGAGTCCGCCTCGGACGACGAGATGTTCGCGCTCATCGACCAGCAACTGGGCTCGTCCTGACGAGAGGTGGGGGAGGACCAATGCCGTCCACGGAAGACAAGCTGCGCCAGTACCTCAAGCGGGTCACCGTCGACCTGGGCGAGGCCCGGCAGCGCCTGCGCAAAGCGGAGCAACGCCACCACGAGCCCATCGCCATCACCTCCATGGCCTGCCGCTACCCCGGCGGCGTCACCTCCCCGGAAGCCCTGTGGGAGCTGGTCGACAGCCGGACCGACGCCATCGGCCCCTTCCCGGCCAACCGCGGCTGGAACCTGGCCGCCCTCTACCACCCCGACCCCGACCACCCCGGCACCAGCTATGTCCGGGAAGGCGGCTTCGTCCACGACGCCGACGAATTCGACGCCTCCTTCTTCAACATCTCCCCGCGCGAAGCCCTCGCCATGGACCCGCAGCAGCGGCTCCTCCTCGAAACCGCCTGGGAACTCCTCGAACGCGCCCACCTCGACCCCACGGCCCTCAAAGGCACCCCCACCGGCGTCTACACGGGCTGTGGTGTGCCCGGCTTCGGCACACCGCACATCGAGCGCAGCGCCGAGGGGTTCCTGCTGACGGGCAACGCGCTCAGCGTCGTGTCCGGCCGTATCGCCTTCACCCTCGGCCTGGAGGGGCCCGCGGTCACGCTGGACACGGCGTGCTCGTCCTCGTTGGTGGCGATGCACCTGGCGGTCCAGGCGCTGCGGCAGGGCGAATGCGATCTGGCCCTGGCCGGCGGGGTGACGGTCATGGCGACCCCGAACGTGATCACGGAGTTCTCCCGGCAGCGGGGCCTTGCCCCGGACGGCCGGTGCAAGCCGTTCGCCACGGCGGCCGACGGCACGGGCTTCTCCGAGGGCGCGGGCCTGGTGCTGCTGGAGCGGCTGTCGGACGCCGAGCGCAACGGGCACCAGGTGCTCGCCGTCATCCGGGGTACGGCCGTCAACCAGGACGGCGCGAGCAACGGGCTGAGCGCCCCCAACGGGCCCTCGCAGCAGCGGGTGATCCGCCAGGCGCTGGCCAACGCCGGGCTGGCCACGGTCGAGGTGGACGCGGTGGAGGCCCATGGCACGGGCACCACGCTCGGCGACCCGATCGAGGCCGAGGCGCTGCTGGCCACCTACGGCCAGGAGCGGGATGAGGACCGCCCGCTGTGGCTGGGGTCGATCAAGTCCAACATCGGCCATACGCAGGGCGCCGCCGGGATCGCGGGCGTCATCAAGATGGTGATGGCCCTGCGCCACGGCTCGCTGCCCGCCACCCTCCACGTCGACGAGCCCACCTCGCATGTGGACTGGAGCCGCGGCACGGTGCGGCTGCTGACCGAGCCGGTCGACTGGCCGGCGGCCCCCGACCGCCCGCGCCGGGCCGGCGTCTCGGCGTTCGGCATCTCCGGCACCAACGCCCACGTCATCCTGGAGGAGGCGGCCCGGCCCACGGCGGTGGAGGCACAGGGCGCCGCGCCGGAGGCCGAGGCGGAGACAGCGGAGGAGGAGGCCGCCGAGCCTGCCGTCCTGGGCGGCGTGGCGCCGTGGCTGCTCTCGGCCC GTACCCCACAGGCCCTGGCCGACCAGGCGCGGGGGCTGGCCCGGGCCGTCACCGGCACCGGCGCCCCGGGCGCCGCGCCCGCCGAGGTGGCCTGGTCGCTGGCCACCACCCGCGCCACGTTCGACCACCGCGCGGTGGTCACCGGCACCGAACTCGCCGACCTGACCGCCGCGCTCGATGCCCTGGCCACCGGCGGCGAACACCCCCGCCTCGTCCGCGGCACCGCCCTCGACCCACAGGCGGGCCCGGTCTTCGTCTTCCCCGGCCAGGGCTCCCAGTGGCCCGGCATGGCCGTCGGACTGCTGGACTCCTCGCCCGCCTTCGCCACCCGGATCGCCGCCTGCGAACGGGCCCTGGCGCCCTACGTCGACTGGTCGCTCACCGCCGTGCTGCGCTCCACCGACACCACCACCGACCCGCACCGCGTGGATGTCATCCAGCCGACCCTGTGGGCCGTCATGGTGTCCCTGGCGGGCCTGTGGCAGGACTTCGGCGTCACCCCCGCCGCCGTGATCGGCCACTCCCAGGGCGAGATCGCCGCCGCCTGTGTGGCCGGGGCCCTCACCCTCGACGACGCCGCCAAGGTGGTGGCCCTGCGCAGCCGGGCGCTGCGCGCCCTCGCCGGCCACGGGGCCATGGCCTCCCTCACCCTCGGCGCCGAGGACACCGCCCGCGTCCTGGCCGGACTCGGCCAGGCGGCCGAGGACGTCGCGGTCGCCGCCCACAACGGCCCCCGCTCCACCGTCGTCTCCGGACCGCCGGACCAGATCGCCACGGTCCTGGCGGCCGCCGAGGCACAGGGCGCCAGGACCCGCACCATCGACGTGGACTACGCCTCCCACAGCCCGCACGTCGACCGCATCCGCGACACCATCCTCGCGCAACTCGCCGACCTGGCCCCCACCACGCCCACCATCCCCTTCTACTCCACCGTCACCGGCGAGCAGCTGACCGGCACCCCGCTCGACGCCGAGTACTGGTTCACCAACCTCCGCCGCCCGGTCCGCTTCACCGACACCCTCACCGCCCTCCTGGACCACCACCACCGCCACTTCATCGAGGCGAGCCCCCACCCCGTCCTCACCCCCGGCATCCAGGACGCCATCGACGATGCCGACCTCCCCGCCACCACGATCCCCACCCTCCGCCGCGACCACGGCACCCCCCACGACCTCGCGGACGCCCTCGCGCTCGCCCACACCACCGGCCTCGCCGTCGACTGGCGGCCCTGGTACGCCGCCACCCCGCCCGCCACCACCGATCTGCCCACCTACCCCTTCCAGCGGCAGCGCTACTGGTCGGCGGCGGGCCGCCGGGCCGGTGACGTCAGCGCGGCCGGGCTGCGGCCGGTGGACCACCCGCAGCTGTCGGCCGCGACGGGCCTCGCCGACGGCGGGCTCCTGCTCACCGGGCGGCTGCCGGCGGCCGGGGACGCCGGCTGGCTGGGCGAGCACGAGTTCGCCGATGCGGTCCTGGTGCCCAGCACGGCCCTGGTGGAGTGGGCGCTGCGGGCGGCCGACGAGGCGGGCTGCGGCGGTGTCGAGGAGCTGACCCTTGAGGTCCCCCTGACCCTGTCCGCCGCCTCCGAGCTGCGCGTCCAGGTCGTGGTGGACGCCCCCGACGAGGACGGCCGGCGGGCCGTACGCATGTCCACCCAGCCCGCGGTCGACACACCGGACGGAGCCGACGGGCCGGACACCTGGACCTGCCATGCGACGGGCACCCTCACGGTCTCCCCAGCCGCCGGGACTGAGCTGGCGGGCGCCTGGCCACCGGCCGGTGCCGAACCGGTGGACCTCACCGGCCTGTACGCGCGCGCCGAGGCCGCCGGCTACCGGTACGGGCCGACGTTCCAGGGCGTCCGGGCGGTGTGGCGGCACGGCGCCGACCTCCTCGCCGACGTGGCCCTGGACCAGGGGGCCGAGGAGGGCGGCGACGAGTTCGGCATCCATCCCGCGCTGCTCGAATGCGCCCTGCACCCGGTGGCGCTGGACGACGCGGACACCGACGGCCCGCTGTGGCTGCCGTTCTCCTGGAGCGGCGTGTCCCTGCACGCGGGCGGCGCCACCGGCGTACGGGTCCGGATCGGACAGCGGGAGCACGCCGAAGGCCGTGAGCTGACCGTCGTCGTCGCGGACCCGGCCGGCGCCCCGGTGCTCACCGTGGATTCGGTCGCGCTGCGGCCCGCCGACGGAGACTGGCTGAAGGCCGCGGAACGCCGCTCCGCCGGCGGCCTGTTCACGGTGGAGTGGACACCGCTGCCGCCGCCGGAGAGCCGGCCCGAGCCGGGTGAGGCCGAGGAGCGCTGGGCGACGCTCGGCGACCTGGGCCCCGGGCGCCACTACGCCGACCTGGCCGCGCTGCTGTCGGCGGTGGACGAGGCAGAGCCCGCTCCGCCCGTGGTCCTCGCCGCCGTGCCACCTGCGGCCGGCTCCGGGGCCGACACGGCTCCGGTCACCGTCCGGCACACGTTGGAGCTGGTCCAGGGCTGGCTGGCGGAGCCGAGGTTGCGGGACTCCCGGCTGGTGCTGATCACCTCCGGCGCGGCCTCGGCCGGCGAGGACGACGGGCCGGTGGATCCGGGGAGCGCGGCCGTGCTCGGCCTCGTCCGGGCGGTCCAGGCCGAGCACCCGGACCGCTTCACGCTGGTCGACCTGGGCGCCGAAGCGGGCGCGGGCTCGGACTCGGGCACGGACTCGGGCACGGGCTCGGGCTCGGGGGCGGAAGAGACGTTGGTCGAGGCGGTGCGCCGGGCCGTCGCCGCGGACGAGCCGCAGATCGCAGTCCGTTCGGGCGCGGTGTCGGTCCCCCGCCTGGTACGCGCCGTCACCCGCCCCGATGAGGACACGGCGGTCGAACTGTCCGGCGGCACGGTGGTGGTGTCCGGCGGGGCGGACCACCCGGCCGGCGGCCAGGTCGCCGAGCAACTGGTGCGCGCGTACGGCGCCGAGCGTCTGCTGCTCCTCTCCCACCCGGACAGCGGGGCCCCCGGCGCGGCCGACCTGGCCGAGCGGCTGACGGCGCTGGGCGCCCGGGCGGAGGTGGCGGCGGTCGATGTCGCCGACCGCTCCGCTCTGGCGGAGGTTCTGGCCTCCGTGCCCGACAGCCACCCGCTGGTGGGCGTGGTGCATCTGGCCGGGGCGTCCGACGGCGCCCCGGTGGAGTCCTGGGACGACGGGCGAGTGTCGCGGGCGTGGGCGCCGGGGGCCACCGGCGTGTGGAACCTGCACGCCCTCACCCATGGCCTGCCGCTGCGGATGTTCGTGGTGTTCTCCTCCGCCGCCGATGTCACGGGCGGCCCCGGCCGGGCCGGACACGCCGCGGCTCACGCCCACACCGACGCGCTGATCGCCCACCGGCGCGCAGCGGGTCTCCCGGGTACGGGCCTGGCCTGGTCCCTGGGGGAAGAGGCCACTCCCGACGCCTCGCACCTGCTCGACGCGGCGTTGCACGCCGTACACCCCCTGGTCGTGGCGGCGGACCTGGACGTCGCCCGGCTGGGGCCCGCGGCTCCCGCGCTGCTGCGCGCCCTGG TCCGGCCCGGCCGGCGCCGCGCCGCGGAACGGCAGTCGGCGGTCCTCGCCCTGGCCTCGCGCCTCGACGGTCTCGACGCCTCCGGACAGCGCGAACTGCTGCTCGATGTGGTGCGCCGGATGGCCGCCGTGGTGCTGGGCCACTCCTCCGACGCGGCGATCCGGGCCGAGGCCGCCTTCAAGGAGCTGGGCTTCGACTCCCTCACGGCCGTCGGGCTGCGCAACCGCCTGGTCGACGCCACCGGGCTGCGGCTGCCCTCGACCCTCGTCTTCGACTATCCGACCCCCCGGGCCCTGGCCGACCACCTCCTGCAGCACCTGACCAGCACCGCGCCCGCCACGGCGGTGCCGGTGGGGCCGGCCCGGGCCGCGAGCGCGGACGACGAGCCCATCGCCGTGGTGGCGATGGCGTGCCGCTTCCCGGGCGATGTGACCACGCCCGAGGGGCTGTGGGACCTGGTCGCGGCGGGAGAGCACATCCTGGGGCCCTTCCCCACCAACCGCGGCTGGGACCTGGCCAACCTGTTCCACCCCGACCCGGAACACCCGGGCACGACCTACGCCTCCCAGGGCGCGTTCGTCTACGACGCCGACGGCTTCGACGCCGCGTTCTTCGGCATCAACCCCCGCGAGGCGCTGGCCATCGACCCCCAGCAGCGTCTGATCCTGGAAACCGCCTGGGAGGCCCTGGAACGCGCCGGTATCGACCCGCACACCCTCAAGGAGAGCCTGACCGGGGTCTACACCGGTGTCATCTACCACGACTACGCCGCCGGTCTGCCCGCGGGCGACCCCCGGCTGGACGGCTACACCATGCTGTCCAGCATCGGCAGCATCATCTCCGGCCGGGTGGCGTACACCCTCGGCCTCCAGGGCCCGGCCGTCACCGTGGACACCGCCTGTTCCTCCTCCCTGGTCGCCATGCACCTCGCCGCCCAGGCCCTCCGTCAGGGCGAATGCGATCTGGCGCTCGCGGGCGGGGTGACGGTCATGGCCACCCCTGACCCGTTCACCGGGTTCTCCCGGCAGCGCGGGCTGGCCCCCGACGGGCGGTGCAAGCCGTTCGCCGCCGCCGCCGACGGCACCAGCCTGAGCGAAGGAGCCGGTCTCGTCCTGCTCGAACGCCTCTCCGACGCCCGCCGCAACGGCCACCAGGTGCTCGCGGTGGTGCGCGGCTCGGCGATCAACCAGGACGGCGCGTCCAACGGGCTGACGGCCCCCAACGGCCCCTCCCAGCAACGCGTCATCGGCCAGGCCCTCGCCAACGCCGGCCTGGGCCCCGCCGACATCGACGCCGTGGAGGCCCATGGCACGGGTACGACGCTGGGCGACCCCATCGAGGCCCAGGCCCTCCTGGCCACCTACGGCCAGGACCGCCCCGAGGACCGGCCCCTGTGGCTCGGCTCGGTCAAGTCCAACATCGGCCACACCCAGGCCGCCAGCGGCATCGTCGGCGTCATCAAGATGATCATGGCGATGCGCCACGGACGGCTGCCCGCCAGCCTCCACATCGACGAACCCAGCCCGCACATCGACTGGACCAGCGGAAACGTCCAGCTCCTCACCGAGGCCACCGACTGGCCCGAGACCGGCCGCCCCCGCCGCGCCGCCGTCTCCTCCTTCGGCGCCTCCGGCACCAACGCCCACCTCATCCTCGAACAAGCCCCCCACCGGCCAGAACCCCGGCCGGTGGCCGGCACTCCCCCGCCCGGCACCGTCCCCTGGCCACTGTCGGCGCGCGACGACCAGGCCCTGCGCGAGCAGGCCGCCGCGCTCGCCGAGCAGCTGCGGGCCGACGGCAGCGCGTCGGTGGCGGCCGTGGGGTGGTCCCTGGCCACGACGCGGGCGGTGTTCGAACGGCGGGCCGTGGTCGTCGGCGAAGGCCTGGAGGAAATGACCGCCGCGCTGGAGGCGCTGGCCGCCGGCGATCCGCACCCCGGCCTGTCCACCCCCACCGGCGCCGGGTCAACGGCGCCGGGGAACGTGGTGTGGCTGTTCTCCGGGCAGGGCAGCCAGCGGCCGGGCATGGGCGCCGACCTCTACCGGCGCTTCCCGGTCTTCGCCGACGCCTTCGACGAGGTCTGCGCGCTCCTGGACCCCCACCTCGACCATCCGCTGCGCGAGGTGGTCTTCGCCACCGACCCCGGACACGGCGACCTGATCCACCACACCACCTACACCCAGGCCGGTCTCTTCGCCCTCCACCTCGCCCTCGCCCGCCTCCTGACGAGCATGGGCCTGGCCCCCGACGCCGTCGCGGGCCACTCCATCGGCGAGATCTCCGCCGCCCACCTCGCGGGCGTGCTCTCCCTGGAGGACGCCGCCCACCTGGTCGCCGTCCGCGCCACCCTGATGGGCGGTCTCCCGCCCGGCGGCGCCATGGCCACCGTCCACGCGAGCGAAGAGGAGATCACCGCCACCCTGGACGACCACCCCGGCCTGGCCATCGCCGCGATGAACACCCCGGGCCACACGGTCATCTCGGGCCCCGCCGACCAGGTGGCCGCCGCCACCGCGAAGTGGCGGGAACAAGGCCGCAGGACCCGCTCCCTCCCGGTCTCCCACGCCTTCCACTCACCCCTGATGGAGCCCATCCTCGCCCCCTTCACCGAGGCCATCGGCCATCTGACCTTCCACCCGCCCCGTACCCCGCTGATCAGCAACCTCACCGGCCGGCCCGCGGGCGAGGAGATCACCACCCCCGAGTACTGGGCCCAGCACATCCGCCGGCCGGTCCACTTCCACCAGAGCGTCAGCCACCTCGCCGAGGACACCGCCGTCTTCCTCGAGCTGGGCCCGGCTCCCGTGCTGACCCACGCCGTCCACCACACCCTGCCCGAGGAGACCAACGCCACCGCGCTGGCCACCCTCACCGGCAAGCAGCCCGATGTGCGGGCCCTGGCCCACAGCCTGGCGTCGCTGCACATCGCCCACGCCCCCCTGGACTGGACGCCCTGGTATCCCGCCGACCCGGCCCCCGAGACGGTCGCGCTGCCCACCTACCGCTTCCAGCGCCGGCCGTACTGGATCGCCCCCCACACCTCCGGCAACGCCGCCCCCGGCGGCACCGGCCTTGACCATCCGCTGCTGGACGCGGCGGCGCCCCTGGCGGACGGCGGCGTGGTGCTCACCGGGCACGTCCCACCGGCCGACCACGACAGCTGGCTCACCGAGCACACCATCGCCGAGACCGTCCTGCTGCCCGGCACCGCGCTCCTCGAACTCGCGCTGTGCTCCGCCGAGCACACCGACAGCCCCCACGTCGAGGAACTTCTCCTCCACCACCCGCTCACCCTCCACCCGACCTCCCCCCTCGATCTGCAGATCGTCGTCGGCACCGCCGATGACGCCGACCGCCGGGTCCTGCACATCTACACCCGCCCCCAGTCGGCCCCCGCGGCCGAGTGGACCCGTCACGCCACCGCCACCCTCGCCGCCGAGCCCGCCACCGGCCATCCGGCGCAGGTGCGGGAGGCGGCATGGCCGCCCGCCGGGGCCGAACCCGTCGACATCACGGGCTTCTACGACCGCGCGGCGGCGAACGGCTAT GCGTACGGGCCCGCTTTCCAGGGGCTCCAGGCGCTGTGGCGGCGCGGCGACGACCTCTTCGCCGAGGTCACCCTGTCTACGGGCGAGGCCACCGACGACACGGCCGAGGCCCTCACCGTCCATCCCGCGTTCCTCGACAGCGCGCTCCACCCGCTGGTCGCCGTCACGGACACCTCGGGCGGCGAGGTCTGGCTGCCGTTCTCCTGGAGCGGCGTCACCCTCCACGCCACCGGCGCCACCCACGCCCGGGTCAGGATCACCTCCCGCAACGAGAGCGAGCACAGCGTCACCCTCACCGACGCCACCGGGCAGCTCGTCCTCACCGCCGACGCCGTCACCACCCGGCCCATGCGCCTGGACGCGCCCCAACCGGCCACCACCGAGGGCCTGTTCACGCTGGAGTGGACGCCCGCGGCTCCCCTCGCCGAACGGGCGGAGGAGCCCGCACCGCCCGAGGTGGTCTTCGCCGAGGCGCCGGTCGCAGACGCTGACGGCGGCGAGCTGGAGGCCGTCGAGCGGGCGTTGGCGCTGGTGCGGAACTGGCTGGCCGAACCACGGCCCGACGACGCCCGGCTGGTGGTGCTGACCCGCAAGGCCGTGGCCGTGGACGAGGAGACGGACCTCGACCCCGTCGCGGCGGCCGTCTGGGGCCTGGTCCGCAGCGCCCAGTCGGAGAACCCCGGCCGCTTCGTCCTGCTGGACACCGACCTGCCCACGGACAAGGACACCGCTCCGGCGTCCGACGGCCGGGTGGCCGACGCGGTCCGCTGCGCGCTCGACCTCGACGAACCGCAGCTGGCGCTGCGCGGCGGCCGGGTCCTGGTGCCCCGGCTCGTCCGCGCCACCGCCTCGGCCGCGCTCGTACCCCCCGTCGGCCAGGGGGCGTGGCGGCTGGACGCCGCGGGCACGACGGGCTCCGCGACGATCGACTCCGTGGCGCCGGTGCCGTTCCCCGAGGCCGAGGAGGAGCTGGCGGCCGGGCGCGTACGCATCGAGGTGCGCGCGGCCGGTGTCAACTTCCGCGATGTCCTGATCAGCCTGGGCATGGTGCCCGACCAGACCCGCATCGGCGGCGAAGGCGCGGGCGTCGTCCTCGAAGTGGCCCCCGACGTCACCACGGTGGCGCCCGGCGACCGCGTCCTGGGCGTCTTCGACGGGGCGTTCGGCCAGGTCACCGTGGCCGATGCGCGGATGGTGACCAGGATGCCGGCAACCTGGGACTTCCCCCGGGCGGCGGCCGTCCCCGTGGCGTTCCTGACCGCGTGGTACGGGCTGGTGGAGCTCGGCCGCCTGAAGGCGGGCGAGTCGGTGCTCATCCACGCGGCGGCCGGCGGTGTGGGCATGGCCGCCGTGCAGATCGCCCGCCACCTGGGCGCCGAGGTGTACGGCACCGCTAGCCCCGGCAAACACGCCGCGCTGGAGGCCATGGGCATCGACGAGACCCACCGCGCCTCCTCCCGCGACCTCGACTTCGAGACCGCCTTCCGCGCCGCCACCGGCGGCCGCGGCGTCGATGTGGTCCTCAACAGCCTGGCGGGCGACTTCATCGACGCCTCGCTGCGTCTGCTGGCCAAGGGCGGCCGGTTCCTGGAGATGGGCAAGACCGACCTCCGCGACCCGGAGCAGGTGGCCGAGGACTTCGCCGGGGTGGACTACCGGGTGTACGACCTGGTCTCGGACGCCGGGGCGGAACGCGTCGCGCTGATGCTGGCGACCCTGGTGGAGCTGTTCGACGAGGGCGCTCTGACGCCGCCCCCGGTGCAGGCGTGGCCGCTGGCCAGGGCCCGCCAGGCGCTGCGCCACATGAGCCAGGCCAAACACACCGGCAAGATCGTCCTGACCGTCCCCCCTGCTCTCGACCCCGACGGCACGGCCCTGATCACCGGCGGCACCGGCACCCTCGGCGCCCTGGTCGCCGAACACCTCGTCACCACCCACCACATCACCCACCTCCACCTCCTCAGCCGCCGCGGCCCGGACGCCCCCGGCGCAGCCGACCTGACCGCCCGGCTCACCGAGCTCGGCGCCACCGTCCGCATCACCGCCGCCGACACTTCGGACCTGCGGGCCGTGCGGCAGGCCGTCGACGCGATCGACCCCGACCACCCGCTCACCGCCGTCATCCACACCGCCGGTGTCGTCGACGACGCGATGGTCACCTCCCAGACCCCCGACACCCTCCGCCGCGTCTGGGCGGCCAAGGCGGCGGGCGCGGCCAACCTCCACCGGGCCACCCGGCACCTCCCGCTGGCCATGTTCGCCCTCTACTCCTCCGTCTCCGGCACCCTGGGCAACCCAGGACAGGCCAACTACGCCGCGGCCAACGCCTACTGCGACGCCCTGGCCTCCCGGCGACACCACGCGGGACTGCCCGCCACCTCCATCGCCTGGGGGCTGTGGGACGCCACCAGCGACATCACGGGAGCGCTCACCGAGGCCGATGTGGCGCGGATGGGCCGCACCGGCGTCACGGCGCTGGCCACCGACCACGCGCTGGCCCTCTTCGACGCGGCCCACCGGCACGGGGACCCGCAGCTCGTGGCGCTCGACCTCGACGTCCACGCGCTGGCCGCACAGCCGGCGGCCACCCTCCCGGCCGCGCTGCGCGCCCTGGCCGCCCGCCCGAGCGGGGCCACGCGGCGCGCCGCCGCGGCCGCGCAGCGCCCCGACGACTGGACGCGCCGGCTCGCCGGACTCCCGGAGGCCGAGCAGCGGCAGCAGCTGCTCACACTGGTGCGCACGAACGCCGCGACGGTGCTCGGCCACGCCGACGCCGAGAGCGTACGGGAGGAGGCGCCGTTCACCGATCTGGGCTTCGACTCGCTGACCGGTGTCGAGCTGCGCAACCGGCTCTCGGCGGCGACCGGGCTGCGGCTGCCCGCCGCGCTGGTCTTCGACTTCCCCTCGGCCCGGGCGCTGGCCGACCATCTGCGCGGCCAGCTGGTGGCGGACGGGGGAGCCGGCGCCCAGGGCGGGGTGGACCCGGTGCTCGGCGAGCTGGCGCGGCTGGAGTCCACACTGTCGGCCCTCGACCTGCCCGAGGCGGACGCGCGGGCGGTGACAGATCGGCTTGAGGGTCTGCTGGCCCAATGGAAGGCGGCGTCCGCGCCGCCGGCCGAGGACAACGCGACCGACCGGCTCACGCTCGCCACCGCCGACGAAGTGCTCGCGTTCATCGACAACGAACTCGGCACCTCGTGAAGGCTCAGTTGCTCGCTTCGACGCGTATGAAGGTCGTGACTGCGGATGCCGGATGAAGAGAGGCTTGTCGACTACCTCAAGCGTGTGGCGACCGATCTGCACGACACGCGGCGGCGCCTGCGCGAGGTGGAGGAGCGGGCCCAGGAGCCCATTGCCATCACCGCGATGACCTGCCGGTTCCCCGGCGGGGTGGATTCGCCCGAGGCGTTGTGGGACCTGGTGGCCTCCGGTGTGGACGCCGTCGGGCCGTTCCCCGCCGACCGCGGCTGGGACCTGGACGGCCTGTACCACCCCGACCCCGACCACCCGGGCACCACGTATACGCGCG AGGGCGGGTTTCTGCGCGACGCCGACAAGTTCGACGCGGGCTTCTTCGAGATCAGCCCCCGCGAGGCGCTGGCCACCGACCCACAGCAGCGCGTACTGCTGGAGGTGGCCTGGGAGCTGTTCGAGCGGGCCGGTATCGACGCCACGGGCCTGCGGTCCAGCCGGACCGGTGTCTTCGTCGGGGCCGCCACCATCGGCTCGGCCACATCGGGTGTCCCCGTCCGCAAGGAGACCGAGGGGTACGCGGGCGTCGCCCCCAGCATGCTGTCGGGCCGGCTGTCGTACACCTTCGGCCTCGAGGGCCCCGCGCTGACGGTGGAGACGGCCTGCTCCGCGTCGCTGGTGGCGATGCATCTGGGCATTCAGGCGCTGCGGCAGGGCGAGTGCGAACTCGCGGTGGCCGGTGGCGTGACCATCATGGCCTCGCCCGCGGTGTTCACCGGCTTCGCCCGCCAGCGCGGTCTGGCGCCGAACGGACGCTGCAAGCCGTTCGCCGCGGCCGCCGACGGCACGGGCTGGGGCGAGGGCGCCGGTCTGATCCTGCTGGAGCGGCTGTCGGACGCCCGCCGCAACGGCCATCAGGTGCTCGCCGTCATCCGGGGCTCGGCCGTCAACCAGGACGGCGCGAGCAATGGCATCACCGCGCCCAACGGCCCTTCCCAGCAGCGCGTCATCCGCCAGGCGCTGGTCAACGCCCGCCTGTCGTCCGCCGAGGTGGACGTGGTGGAGGCACACGGGACGGGCACCCGGCTCGGCGACCCGATCGAGGCGGACGCGCTGCACGCGACGTACGGGCGGCAGCGGCCGGCCGACCGGCCGCTGCTGCTGGGCTCGCTCAAGTCGAACATCGGCCACACCCAGGCCGCGGCGGGGGCGGCGGGCGTCATCAAGACGGTGATGGCGATGCGCCACGGGACGATCCCCGCGACGCTGCACATCGACGAGCCGACGCCACATGTGGACTGGAGCTCGGGGGCCATCCGCCTGGTGACCGAGCCGGTCGACTGGCCGGAGACGGACCACCCGCGCCGGGCCGGGGTGTCCTCGTTCGGCGTGTCCGGGACCAACGCACATGTGATCATCGAGCAGGCACCCGACGCGGCCGAAACCACCCCGGCCGGCCCCGCCCCGGAGAGCCAGGAGGGCGCCCCGGAGGCGAACCGAGCCGCAATAAGAGGCACCGTGGTGCCGTGGGTGCTGTCCGCGCGGGGCGAGGCGGCGCTGGCGGAGCAGGCGCGGCGGCTGGCCGAGGCGGTGGAGCGCGATCCGGCCGCCGAGGTGGCGGACGTGGGCTGGTCGCTGGCGACGGCGCGGGCCCGGTTCGAGCACCGGGCCGTGGTGGTCGGCACGGACCGGTCGGCGCTGCTGGGCGGTCTGGCCAAGCTGGCCGCCGACGAGCCCGATCCCGCGGTGGTCACCGGCGCGGCGGGCCCGACGGGCGTCGGCCCGGTGTTCGTCTTCCCCGGCCAGGGGGCGCAGTGGCCGGGCATGGCGCGTGAACTCCTCGACTGCTCCCCGGTCTTCGCCGCCCGGATCGCCGAGTGCGAGCGGGCCCTGGCGCCGTACGTGGACTGGTCGCTGACCGCCGTGCTGCGCGGCACGGACGGTGCGACGGACCCCAAGCGGGACGATGTCGTCCAGCCCGTGCTGTGGGCCGTACACGTCTCGCTGGCCACGCTGTGGCAGAGCTTCGGCATCACCCCGGCGGCCGTCGTCGGGCACTCCCAGGGCGAGGTCGCCGCCGCGTGTGTCGCCGGGGCCCTCACCCTGGACGACGCCGCCAGGATCATCGCCCTGCGGGTCCAGGCCCTGCGCCCGCTGATCGGCCACGGCGCCATGGCCTCCCTCACGCTGAGCGCCGAGGACACCGCCCGGCTCATCGGCGACCTCGGCCCGGCGGCCGGGGACGTGGCCGTCGCCGCCGTCAACGGCCCGCGGGCCACGGTCGTCTCCGGCTCCCCGCGGCCTCTGGCCGCCGTGCTGGCGGCGGCGGAGGAGCGCGGCGCGCGCACTCGGACCATCGACGTGGCGTACGCCTCCCACGGCCCGCACGTGGACCGCATCCACGACGACGTCGTCTCGGCGCTGGGTGAGGTGTCCCCGGTCGAGTCCGAGATCGCGTTCTACTCCACCATGACCGCGGAACGCCTGAACACCGCGGGGCTCGACACCGAGTACTGGTTCGACAACCTCCGCCGGCCGGTGCGTTTCGCCGAGGCCGTGGACCGGCTGCTGGCCGACGGATACCGGGTGTTCATCCAGTGCAATCCGCACCCCATCCTGTCCACGAGCCTGCAGGAGACCTTCGAGGAGTCCGGCGTCCCCGCCACCTCCCTCGCCACCCTCCGCCGCGACCACGGCGGCGCGCACCAGCTGGCCCTCGCGCTCGCCGAGGCGCACGCCGCGGGTCTCGGGGTCGACTGGCGCCCCTGGTTCGCGGGCGACGGCGTCCCCCGCACGGTCGAGCTGCCCACCTATCCCTTCCAGCGCCGCCGTTACTGGATACCGTTCGGCGGAAAGGGCACCGGCGACATGTCCGCCGCCGGTCTGCGCGCGGTGGAACATCCGCTGCTGGCCGCCGCGGTGAGCCTGCCCGACGGCGGCATGGTGCTGACCGGGCGCCTTCCGGCCACGGGCGACGGCTGGCTCGCCGACCATGTCGTCGGCGGCACCGCGCTGCTGCCCGGGGCGGCCCTGGTCGAGTGGGCGCTGCAGGCCGCGCACGAGGCGGGCTGCGCCGCGGTGGAGGAGCTGGCCCTGCGGATCCCGCTCGTGCTGCCCGCCTCGGGGGCCCTGCGCATTCGTGTCGCCGTGGGGCCCGCCGACGACGACGGCAGGCGGACCGTGGATGTGCACTCGCGCCCCGACGAGCTGGACACGGAGGCGCCCGACGGGTGGGTGTGCCACGCCATGGGCACGCTCGCGCCCGAGGACGCCGCGGACCCGGCCGCCCCGCCGGACACCGCGGCGGCGCCCTGGCCACCGCGGGCGGCGGAGCCGCTGGACGTCGCGGACTTCTACGAACGGGCCGCGGACGGCGGCTACGGCTACGGCCCCGCGTTCCGCGGTCTGACCGCGGCGTGGCGGGACGGCGCCGATCTGCTCGCCGAGATCGCGCTGCCCGAGGCGGCCGGCGAAGGCCCCGACCGGTTCGGCATCCACCCGGCGCTGCTGGACGCGGCCCTGCACCCGACGCTCCTGGACGGCGGCCGTCAGGGCGCGTCGCGGGACGGTCAGGTGTGGCTGCCGTTCGCCTGGAGCGGCGTGTCGCTGTGGGCCACGGGGGCGCGCCGCGTACGGGTGCGCGTATCCCCGCTGGAAGGCGGGCGGCGGATCTCGCTGACCGACGAGACCGGCGCGCCGGTCCTGGACGCCGAGTCGGTCTCCGTACGCCCCACCGGCGTCGCCGAGCTGCGCGCCATCGGCGAGCGCGCGGCCGAGGGCCTGTTCGTCGTGGACTGGGTGCCCGCGCGTGGCGGGACGGGCGACGCGCCGGCGCTGCCGGACGACGGCTGGGCGATCATGGGCGACGGCT GGGGAGCAGCGGGCGACGGTGGCGTACTCGCCGGGGTGGCGGCCCACGCCGACCTCGGCGCGCTGCTCGCCGCGGTGGACGACGGGACCCCGGTGCCGACGGTCGTCCTCCACCCGGTGACAGCGCCTGCCGCCCCCGACGGCGGCCTGGCCGCCGTCGGGGGCGTGCTGAGCCTCGTCCAGGGGTGGCTGGCGGAGCCGCGGTTGGCGGACTCCCGGCTGGTCCTGGTCACCTCGGGCGCCGTGTCGGCCGGCGACGACGAGGGGTCGGTGGATCCGGGCGGCGCGGCCGTGTGGGGTCTGGTCCGTTCGGTCCAGGCCGAGCACCCGGGCCGGTTCACCCTGGTCGACACGGCCGGTGCGGCGGACGGGGACCGGCTCCTGGCCGAGGCGGTGCGCCGGTCTCTCGACGCCGACGAGCCGCAGGTGGTCGTGCGCGCGGCCGGAGCGCTGGTGCCGCGCCTGGTGCGCACCTCTCCGTCGGCCGAGGACGAGGCCCCGGAGCTGTCCGGTGGCACGGTGGTGGTGTCGGGCGGGACGGGGGTGCTGGGCGGTGAGGTCGCCGAACACCTGGTGCGTACGCATGGGGTGGAGCGTGTGCTGCTCCTGTCCCGGCGCGGCGCCCAGGCGCCCGGCGCGGCCGAACTCGTGCGGCGGCTGGTCGACTTGGGGGCCGAGGCGGAGGTGGCGGCGGTCGACGTGGCCGACCGCGCCGCGCTGGCCGAGGTGCTGGCGGCGGTCCCCGAGGACCACCCGCTGGTGGGTGTGGTGCACGCCGCCGGTGTGATCGACGACGCCCTGGTGGAGTCCTGGGACACCGGCCGGCTCGCCCGCGTCTGGACCCCCAAGGCCCTCGGCGCGTGGAACCTGCACACTCTCACCCGTGATCTGCCGCTGCGGCTGTTCATGGTGTTCTCCTCGGCCGCCGGCGTCGTCGGCAACTCCGGCCAGGCCGGATACGCCGCCGCCAACGCGTACACCGACGCCCTGATCGCCGACCGCCGCGCGGCCGGGCTGCCCGGCACCTCGATCGCGTGGACCCTGTGGCAGCAGACCTCGGCCATGACCGAGCACCTGACCGAGGCCGATCTGACCCGTCTGGGCACCCTCGGCATGCGTCCCCTCGCCACGTCCCGTGGCCTCGGGCTCCTCGACGCGGCGCTCCATGTCGCCCACCCGCTGGTGGTGGCCGCCGACCTCGACGCCACCCGGCTCGGCCCCGACGCCCCCGCCATGCTGCGCGCCCTGGCCAGGCCGGCCCGCCGCCGCGCCATGGAACACCACGCCACCGGCCCCAAGCTGGCGGGCCGGCTGGCCGGTCTCGACGACACCGCCCGGCGCGATCTGGTGCTCCATACGGTGCGCCAGATGGTCATGGTGGTGCTGGGCCACTCCTCGGACGCGGCGATCCGCGCCGAGGCCGCGTTCAAGGAGCTGGGCTTCGACTCGCTGACCGCCGTCGAGCTGCGCAACCGCCTGGCAGGCGCCACCGGGCTGCGGCTGCCCGCCACCCTGGTCTTCGACTATCCCACCCCCCAGGCCCTCGCCGACCACCTGTTGGAGCGGCTGACCGCCACCGCCGCGCCCGCGCCCCGCGGTGCCGCGCCGTCCAGGGCCCGGGCCGCCGACGAGCCGGTCGCGGTGGTGGCGATGGCGTGCCGCTTCCCCGGTGGCGTGAGCTCGCCCGAGGGGCTGTGGGACCTGGTCGCGGCGGGACAGCACGTACTCGGGCCCTTCCCCACCAACCGCGGCTGGGACCTGGCCAACCTGTTCCACCCCGACCCGGAACACCCGGGCACGACCTACGCCTCCCAGGGCGCGTTCGTCTACGACGCCGACGGCTTCGACGCCGCGTTCTTCGGCATCAACCCCCGCGAGGCCCAGGCCATGGACCCCCAGCAGCGCGTCCTGCTGGAGACCGCCTGGGAGCTCCTGGAGCGCGGGGGTATCGACCCGCACACCCTCAAGGACAGCCTGACCGGCGTCTACACCGGCGTCATGTACCACGACTACGGCAGTGGTCTGCCCGCCGGGGACCCCCGGCTGGACGGCTACGGCAATCTCGCCGGCACCAGCAGCATCATCTCCGGCCGGGTGGCCTACACCCTCGGCCTCCAGGGCCCGGCGGTCACCGTGGACACCGCCTGCTCGTCGTCGCTGGTCACCATGCATCTGGCCGCCCAGGCGCTGCGGCAGGGCGAATGCGATCTGGCGCTGGCGGGCGGGGTGACGGTACTGGCCACCCCCGATGTGTTCACCGGCTTCTCGCGGCAGCGCGGGCTGGCCCCCGACGGGCGGTGCAAGCCGTTCGCCGCCGCCGCCGACGGCACGGGCTTCGGTGAGGGCGTCGGCCTGGTGCTGCTGGAGCGGCTGTCGGACGCCCGGCGCAACGGGCGGGTCATCCTGGCCGTCATCCGGGGTTCGGCGGTCAACCAGGACGGCGCGAGCAATGGGCTGACCGCCCCCAACGGCCCCTCCCAGCAGCGGGTCATCCGCCACGCCCTGGCCGGCGCGGGGCTCACCCCGGCGGATGTGGACGCCGTGGAAGCCCATGGCACGGGTACGACGCTGGGCGACCCCATCGAGGCCCAGGCGCTGCTGGCCACCTACGGCCAGGACCGCCCCGACGACCGGCCACTGTGGCTGGGTTCTGTGAAATCGAACATCGGGCACACCCAGGCCGCCGCCGGTGTCGCGGGCGCCATCAAGATGATCATGGCGATGCGCCACGGACGGCTGCCCGCCAGCCTCCACATCGACGAACCCAGCCCGCACATCGACTGGACCAGCGGAAACGTCCAACTCCTCACCGAGGCCACCGACTGGCCCGAGACCGGCCGCCCCCGCCGCGCCGCCGTCTCCTCCTTCGGCGCCTCCGGCACCAACGCCCACCTCATCCTCGAACAAGCGCCGCACACACACCAGCCGGTGGCCGACACTCCCCCGCCCGGCATCGTCCCCTGGCCGCTGTCCGCCCGGAGCGATGACGCCCTGCGCGCCCAGGCCACGGCGCTCGCCGAGCGGCTACGAGCCGATGGCCGGCCCGCCGAGCCCCTGTCGGTCCCGGGCGTCGGGCGGGCCTTGGCCGCGACGCGTTCGGTGTTCGAACGACGGGCGGTCATCGTCGGCAGCGACCAGCAGGAGATGACGGCCGCCCTGGACGCCCTGGCCGAGGGGCGCCCCCACCCCGGGCTGATCACCGCCGCGCCAGGCGCCACGCCCACATCAGGCCGGACCGTATGGCTGTTCTCCGGACAGGGCAGCCAGCGCCCCGGCATGGGCGCCGACCTGCACGGGCGATTCCCGGTCTTCGCCGAGGCCTTCGACGAAGTATGCGCGCTGCTCGATCCGCACCTGGACCACCCGCTGCGGGACATCGTCTTCGCCACCGGCGACGAGACCGCCGACCTCATCCACCACACCACCTACACCCAGGCCGGTCTTTTCGCGGTGCAGGTGGCACTGGCCCGGCTGCTGAAGGAGTGCGGTGTGCGGCCGGATGCGGTGATCGGCCACTCGATCGGCGAGATCACCGCCGCC CATATCGCCGGAGTCTTCTCCCTCGAAGACGCCTGCCGCCTGGTCGCCGCCCGCGCCACCCTCCTGGGCAGTCTGCCCCCCGGCGGCGCCATGACCGCCCTCGAAGCCACCGCCGAGGAGGCCGCCGAAGCCCTCGCCCCCTACGACGGCCAGGTCGCCATCGCCGCCCTCAACGCCCCCACCAGCACCGTCATCTCCGGCCCACTGGACCTGGTGACCAAGATCGGCACGGCGTGGAAGGAACGCGGACGCAGGACCAAGGCGCTGACCGTCAGCCACGCCTTCCACTCCCCCCTGGTCGAACCGGTCCTGGACGACTTCCGCGACGCCGTCGCCGAGCTGACGTACCACCAGCCCAGCGTCTCCCTCATCAGCAACGTCACCGGCGAACCGGCCGACGAGACCATCACCACCCCCGACTACTGGGTGCGCCACATCCGCCAACCCGTCCGCTTCCACCCCGCCATCACACACATCGCCCCGCACACCGCCGCCTTCCTCGAAGTCGGCCCCGACGCCGTCCTCGTCCCCGCAGCCCAGGACGCCCTCGACGCGCTGGACGACCTGCCCGCACACCCACCCCTGCTGATCCCGACCCTGACCCGCGGCCAGCCCGACATCCGCGCCCTGGCCCATGCCCTGGCCCGGCTGCACACCCACGCCCCCGTCGACTGGGCCCCCTGGTACCCCGCCGGCCCCACGCCCCCTGGCGTCGACCTGCCCACCTACCCCTTCCAGCGCCGGCCCTACTGGCTGACCCCGACCCACGCGGGGCCGGCCACCCCCGGCGCCGCCTCGCTCACCCACCCCCTCCTCGCCGCCACCGCGCCGCTCGCCGACGGCGGCATCCTCCTGACCGGGCAGGTGCCCGCCGCCGGCCACGAGGGCTGGCTCAGCGAGCACACCATCGCCGGCGCGGTCCTCCTGCCCGCCACCGCCCTCCTCGACATCGCCCTGCACGCCGCGGACCACGCCGCCACCCCCCACATCGACGAACTCCTCCTCCAACAGCCCCTCACGCTCGACCCCACGCACCCGCTCTCACTCCAGGTCATCGTCAGTCCACCCGACGACACCGGTCACCGCACCCTGGACATCTACGCCCGCTCGCAGTCCGGCCCCACGGCGGAGTGGACCCACCACGCCGCCGCCACCCTCACCGACGAGCCCACGGCCGCGCCGCCCCGGGACGGAGCGGAGGCGGCCTGGCCACCCCCGGGAGCCGAACCCGTCGACATCACCGGCTTCTACGACCGCGCCGCCGCGGGCGGCTACCACTACGGACCCAGCTACCAGGGCCTGCGGGCCGTCTGGCGCCACGGCGACGACCTCCTCGCGGACATCGAGCTCCCCGCCGCCGACGCCACCGACCCCACCGCCGATCCCCTCGCCCTCCACCCCGCCCTCCTCGACGCCGCGCTCCACCCGCTCCTCGCCACCGCCGACAACCCGGCCGGCGAGATCTGGCTCCCCTTCAGCTGGGGCGGTGTCACCCTGCACGCCACCGGCGCCACGCGGGCCCGCGTCCGGATCACCCGCCGTGGCGACAGCGAGCTCGGCCTGGCCCTCCTGGACACCACCGGGCGGCCCGTTCTGACCGCCGACACCATCACCACCCGGCCCATCGACACCGCGCGCCTGCGGACCAGCGGGCCGAACGACGGTCTGTACCAGGTGCGGTGGATCGCGCTCCCGATCCCGGCGGAGCCGCCGACGGCCGAAGCGGACGGCTGGGCGGTGCTCGGGGACGGCCGGCTCGACGAGGCGGTGCCGTCGCTGTCCCGGTATCCGGATGTCGCCGCGCTGATCGCGGCCATGGACGACGGCGCGCCCGTGCCGCAGACCGTCCTGACCGGCCCGGCACCCGCCGAGGGCGGCAGCGACGGCGACGGCTCCGATGTGGTGGCGCGGGTTCTGACGACGGCGCAGGAGTGGCTGGCCGAGCCGCGGCTCGCCGGCTCCCGGCTGGTGGTCGTCACCCATGACGCCGCCGCCGTGGCGCAGGACACCCCCGCCGGCCCGGAGGGCGGCGCCGTGGATCCGGTGGCCGCGGGCGTATGGGGGCTGGTCCGCAGCGCCGAGGCGGAGCACCCCGGCCGGTTCGCGCTGCTCGACCTCAGCGGCCGCGACGCCGGTCCGGCACCGGATGTCGTGGGGGTGCTGGGCGCGGCCCTCGCCGCGGACGAGTGGCAGGTGGCCGTGCGCGGCGGGGCGGCGTGGGTGCCGCGGCTGACGGCCGCGGACGCGACCGCCGACATCGTGCTGCCCGTGACCTCGTCCGCCTGGCAACTCGTCATGGCGGACGAGCACGCCGGAACGGTCGACGGGGTGGCGCCGGAGGAGTGCCCCGAGGTGCTGGAGCCGCTGGCGCCCGGGCAGGTGCGCATCGCGGTCCGCGCCGCGGGCGTCAACTTCCGCGATGTGATGGTGACCCTGGGCGTCGTGCCCGACCGGCGCGGGCTGGGCGGCGAGGGCGCGGGCGTGGTGCTGGAGGTGGCCGGGGACGTCACGTCGGTGGCCGTCGGCGACCGCGTCATGGGCCTGTTCCAGGGCTCGTTCGGGCCCGTGGCCCTCGCCGACGCGCGCGCCGTCGTCCCGATCCCGCCGGGCTGGACCGACCGGCAGGCCGCGGCCGTGCCCATCGCGTATCTGACCGCCTGGTACGGACTGGTGGACCTCGCGGGCCTCAGGGCGGGCGAGTCGGTGCTCATCCATGCCGCCACGGGCGGCGTGGGCACGGCGGCCGTGCAGATCGCCCGCCACCTGGGTGCCGAGGTGTACGCCACGGCCAGCCCCGGCAAACACGCCGCGCTGGGGGCCATGGGCATCGACGAGCCCCACCGCGCCTCCTCCCGCGACCTCGACTTCGAGGAAGCCTTCCGCACCGCCACCGGGCCCGAGGGCATGGACGTGGTGCTTGACTGCCTGGCGGGCGAGTTCGTGGACGCCTCGCTGCGGCTGCTGGGCCCCGGTGGCCGGTTCATCGAGATGGGCAAGACCGACATCCGCGACCCCGAGCAGATCGCCGACGCCCATCCCGGCGTCCACTACCGCGCGTACGACCTGGTCTCCGACGCCGGCCCCGACCGGATCTCGGAGATGCTGGACACGCTCGCCGACCTCTTCGCGCGGGGCGTCCTGGCGCCGCCCCCGGTGCAGGCGTGGCCGCTGGCCAGGGCCCGCCAGGCGCTGCGCCACATGAGCCAGGCCAAACACACCGGCAAGATCGTCCTGGACATTCCCCCCGCCCTCGACCCCGACGGCACCGTCCTGATCACCGGCGGCACCGGCACCCTCGGCGCCCTGACAGCCGAACACCTCGTCACCACCCACCACATCACCCACCTCCACCTCCTCAGTCGCCGGGGCCCGGACGCCCCCGGCGCAGCCGACCTGACCGCCCGGCTCACCGAGCTCGGCGCCACCGTCCGCATCACCGCCACCGACACCACCGATCCCCAGGCCCTTGGGCAGGCCCTCGACACCATCGACCCCGAGCACCCCCTCACCGCCGTCGTCCACACCGCCGGCACCGTCGAGGA CGCCGTGCTCACCTCTCAGACCCCCGACACCCTCCGCCGCGTCTGGGCGGCCAAGGCGACGAGCGCGGCCAACCTCCACCGGGCCACCCAGGACCTCCCCCTGGCCATGTTCGTGATCTTCTCCTCGGCCGCCGGCACCCTCGGCAGCCCCGGGCAGGCCAACTACGCCGCCGCCAACGCCTACTGCGACGCTCTCGCCACCCAAAGGCACCACGCGGGACTGCCCGCCACCTCCATCGCCTGGGGACTGTGGGCCGCCACCAGCGGCATGACCGGAGCGCTCACCGAGGTCGACCACGCCAGGATGAGCCGGTCCGGGATGGTGCCGCTGGCCACCGACCACGCCCTGGCGCTGTTCGACGCGGCACACGCACACGGCATGGCGCGGCTGCTGGCCGCCCAGCTCGACCTGGCGGCGCTGTCCGCCCAGCCCGCCGAGGCGCTGCCGCCGCTGGTCCGTTCGCTCACGAGCGCCGGGGCCCGGGTCGTACGGCGGTCCGTGGCGGCGCCGGTGGCCGACCTGTCCGGCCGACTGGGGTCGATGGGCGCCGACGAACAGCTGGCGCTCCTGCTCGATCTGGTCCGCACCCACGCGGCCACCGTGCTGGGGCACGCGGACCCCGGGGCGGTGGCGATGGACACGCCGTTCAAGGATCTCGGGTTCGACTCGCTGACGGCGGTCGAACTGCGCAACCGGCTCACCATGGCGACGGGGCTGCGGCTGTCCGCCGCGTCGGTGTTCCGCCACCCGACGCCCGCCGCCATGGCCGAGCACCTGCGGGGCGAGCTGTGCCCGGCGGAGGACGACGCGCTGCGGCCCGTGCTGCGGGAGCTGGAGCGGCTGGAGGCGGCGGTGGGCGAGGTGAAGCCGGAGGGCGAGACCGGTGCCCAGCTCGTCAAACGGCTGCAGTCGCTGTTGTGGCGGCTGGGCGACGGCGCCTTGGGGGTCGATCACACCGTCGACGGCGAGGCGCTGGAGTCGGCCTCGGACGACGAGATGTTCGCGCTCATCGACCAGCAACTGGGCTCGTCCTGACCGGCAGCGAGGGAGAAGCCATGTCCACCACCGAAGACAAGCTGCGCCAGTACCTCAAGCGCGTCACCTTCGACCTCGGCCAGGCCAAACAGCGGCTGCGCGAAGCGGAAGAGCGCCACCAGGAGCCCGTCGCGATCACCGCCATGGCCTGCCGCTACCCGGGTGGGGTCACCTCCCCGGAAGCCCTGTGGGAGCTGGTGGCCGACGGCACCGACGCCATCGGCCCCTTCCCCACCAACCGGGTCTGGGACCTCGACCGCCTGTACCACCCCGACCCCGACCACTACGGCACCAGCTATGTGACCGAGGGCGGCTTCCTGCACGACGCCGACCGGTTCGACGCCTCCTTCTTCAACATCAGCCCCCGCGAGGCCCTGGCCATGGACCCCCAGCAGCGCGTCCTGCTGGAGACCGCCTGGGAGCTGCTGGAGCGCGCGCGGATCGACCCCACCAGCCTCAAGGGCTCCGCCACCGGCGTCTACACGGGGGTGTCGAGCCAGGACTACCTGTCGCGGATACCGCGGATCCCCGAGGGGTTCGAGGGCTATACGGCCACGGGCGGACTGACCAGCGTGGTGTCCGGCCGCGTGGCGTACACGCTGGGTCTGGAGGGCCCGGCGGTCACCCTGGACACGGCGTGCTCGGCGTCCCTGGTGGCGATGCACCTCGCCGGGCAGGCGCTGCGGCAGGGCGAGTGCGACCTGGCCCTGGCCGGCGGTGTGACCGTGTTCAGCACTCCCACCGCGTATGTGGAGTTCTCCCGGCAGCGGGGGTTCGCGCCGGACGCCCGCTGCAAGGCGTTCGCCGCGGCCGCGGACGGCACCGGTTTCTCCGAGGGCGTGGGCCTGGTGCTGCTGGAGCGGCTGTCGGACGCCCGGCGCAACGGCCATCGCGTGCTCGCCGTGCTACGCGGCTCGGCGGTCAACCAGGACGGCGCGAGCAACGGGCTGTCCGCGCCCAACGACGCCGCCCAGGAACGTGTGATCCGGCAGGCGCTGGACAGCGCCCGGCTCACCGCCGACCAGGTGGACGCGGTGGAGGCGCACGGCACGGGCACCACGCTCGGCGACCCGATCGAGGCCCAGGCGCTGCTTGCCACCTACGGCCAGGACCGCCCGGCGGACCGGCCGCTGTGGCTGGGGTCGGTGAAGTCGAACATCGGGCACACCCACGCGGCGGCGGGCGTGGCGGGCGTGATCAAGATGGTGATGGCCCTGCGCCACGGCCGGCTGCCCGCCACCTTGCACATCGACGAGCCGACCTCCCATGTGGATTGGGACTCCGGCGCCGTACGGCTGCTGACCGAGCCGGTCGACTGGCCGCGGGGGGACCGTCCGCGCCGGGCCGGTGTGTCCTCGTTCGGCATCTCCGGCACCAACGCCCACGTCATCCTGGAGGAGAACACCCAGCCCGCCGGCACTCCCCCGGACGCGGCCGGCGAACGGCCCGAGGACCGGGTCACGCCGTGGGTGGTCTCGGCGCGCGGCGCGGACGCGCTGCGCGCCCAGGCCGGGCGGCTGCTGGACGCGGCGGCTCTCGACGGCGATCCGGACGCGGTGGGCTGGTCGCTGGCCACCTCGCGCACGGTCTTCGACCACCGGGCCGTGGTCATGGGCCGGGACACCGCGGCGCTGCGGGCGGGCCTGGCCGCGCTGGCGGCCGGGGAGGACCATCCGGCGCTGGTACGGCGCGAGGCGGGGGTGCGGGGCTCCGGGTGGCGGGTGTTCCTCTTCTCCGGGCAGGGCAGCCAGCGGCCGGGCATGGGCGCCGACCTCTACCGGCGCTTCCCGGTCTTCGCCGAGGCCTTCGACGAGGTGTGCGCGCTGCTCGATCCGCAGCTGGCCCACCCGCTGGCCGATGTCGTACTGCGCGGCCGGCCGCGAGCGGACCTGCTGAACCACACCACCTACACCCAGGCCGGGCTGTTCGCCGTGCAGGTCGCACTGGCCCGGCTGCTGAAGACCTGCGGCACCCGCCCGGACGCGGTGATCGGCCACTCCATCGGCGAGATCGCCGCAGCCCACATCGCCGGAGTCTTCTCCCTCCAAGACGCCTGCCACCTCGTCGCCGCCCGAGCCACCCTCCTCGGCAGCCTGCCCCCGGGCGGCGCCATGACCGCTCTCGAAGCCACAGAAGAAGAAACCACCGAAGCCCTCGCCCCGTACGACGGCCAGGTCACCATCGCCGCCCTCAACGCCCCCACCAACACCGTCATCTCCGGACCGGCCGACCTCGTCACCGAAATCGGCACGGCCTGGAAGGAACGCGGACGCCGAACCAAAGCCCTCACCGTCAGCCACGCCTTCCACTCCCCCCTGGTCGAACCGATCCTGGACGACTTCCGCGACGCCATCGCCGGCCTGACCTACCACCAGCCCACCATCCCCCTCATCAGCAACCTCACCGGCGAACCAGCCGGCCAGGACATCACCACCCCCGACTACTGGGTGCGCCACATCCGCCAACCCGTCCACTTCCACCCCGCCGTCACACACATC GCCCCGCACACCGCGGCCTTCCTCGAAGTCGGCCCCGACGCCGTACTGATCCCGGCCGCGCAGAACACCCTCGACGCGCTGGACGGCCTGCCCGCGCACCCTCCCCTGCTGGTCCCGGCCCTGACCGGCAAGCAGCCCGACGTCGAGGGCCTGGCCCACGCGCTCGCCCGGCTGCACACCCTCACCCCCGTCGACTGGCGCCCGTGGTTCACCGACGGTGCCCCGCCGCCGACCGTGGACCTGCCCACCTACCCCTTCCAGGGCGAGCGGTACTGGCTGCCGGACGCCCTGGCGGACGCGCCGCCGGCGGAGGCGGACGAGGAGGAGGTCCGGTTCTGGAGCGCGGTGGAGGCGCAGGACCTCCCGGCGCTGTCCGAGGCGCTGGGCATCGGCGAGGAGGACGGGCGGCGCTCGTCGCTCGGCGCGGTGCTGCCGACGCTGTCGCGCTGGCACCGGGAGCGCCATGAGCGGTCGACCCTGAGCTCCTGGCGCTACCGGGTCGGCTGGCGCCGGCTGCCGGAGCTCGGCCCGGCGGCCACGGCGGGCCCGTGGCTGCTGGTCGTACCCCCCCGGGGCGCCGGGGAGGCCGAGGAGTGGGCCGACGCCTGTGAGCGGGCCCTCACGGCGGACGGCGGTGCGGTGTGGCGGCTGGTGACGGACGGCCGGGCGGAGGTGGCCGGGCTCGCCGCGTCGCTGCGGGTGCTGTGCGACGAGGGCGGGGCCCCGGCGGGGGTGCTGTCCCTGCTGGCGCTGGACGAACGCCCACACGAAGCGTTCCCGGCCGTGATCGGGGGTGTGACGGGCACCCATGTGCTGCTGCGGGCCCTGCTGGAGGCGGGGCTCGACGCGCCCCTGTGGTGCGCCACGCGGGGCGCGGTGGCCGTCGCGGACGGCGAAGGGTCACAGGCGCCCGCGCAGGCCCAGGTGTGGGGGCTCGGCCGGGTGGCCGCGCTGGAGCATCCGGCGGCGTGGGGCGGGCTGGTCGACCTGCCCGCCTCGCCCGAGGACCTCGCCCCCGATCTGCTGCGCGCCGTGCTGGCGGGGCGGGCCGGGGAGGACCAGGTGGCGCTGCGGCCGGCCGGGGCCTTCGGGCGGCGGCTGCTGCCCGCACCACTGAGCGGCCTCGGGGAGGCGCCGGCGCGTGAGTGGACTTCCCGGGACGGTGTGCTGGTCAGCGGCGGGGTCGCCGGGACCGCGGCTCTCGTGGCGCGGTGGCTGGCCGCGGGCGGCACCAAGCGCCTCGTCCTGCTGGCGCCGGACGGCCCGGCCACGCCCGGTGGCGCGGAGCTGGTGGCCGAACTGGCGGAGCTGGGCGCGGAGGCGACGGTGGTGGACGGCGTGCCGTCCGAACCCATGGCGCGGCGGCAGCTGGCCGACCGGCTCGCGGCCTCGGGGCTGCGGGTCCGTACGGTCGTCCACACCGGGGCGCCGAGCGACTGGGCGCCGCTGGCGGAGTTCACGCCGGACGGGCTGGCCGAGGCGGTGTCGAACGCCATGGGGGGAGCCGACCGGCTCGCGGAGCTGTGCGGCCTGGAGCCCGACGATCCGGTGGTGGTCTTCTCGTCCATCGCCGCGGTCTGGGGCGGTGGCGGGCATGGCGCCCGGGCTGCGGCCGACGCGTATCTGGACGCGGTGGCGCGGCGCCGTCAGGCGGCCGGCGGCCATGTCGTCCGGCTGGCGTGGGGCGTGTGGGACGGTTCGGAGGGCCCGGAGGCGGCCGAGCGCGCCGAACGGCTGGGGCTGCCGGCCCTCCCGCCCGAGCTGGCGCTCGCCGCGCTGCGGCGCACCCTCGACGAGGGCGGTCACGGCGGCGAGCAGTGCGACGCCGAGGGCACCCGTGAAGGGCGGGGCGACGCGCACGCGGTGGTCGCGGACATCAACTGGGAGCGTTTCGTCCCGCTGTTCACCATGGCGCGCCCCAGCCGTCTCTTCGACGAGATACCGGCGGCCCGGCGGGCCTGGCAGGCGGCGCTCGACGCCCCGGACGAGGAGGGTTCCGAGAGCCTGACGGCCCTGCGGGAGCGGCTGGCGGCCCAGGCGCCCGAGGCCCGCGCCGGCACGCTGCTGGCGCTGGTGCGCTCCCATGTGGCGGGTGCGCTGCGCTACCCGGCCGCGGAGTCGGTCGACCCGGACCAGCCCTTCAAGGAGCTGGGTTTCGACTCCCTGGCGGCCGTCGAGTTCAAGAACCGGCTGCGCGGCGCCATCGGCCTGAGCCTGCCCGCCACGCTGGTCTTCGACTATCCGACGCCGACCGCGCTGGCCGGCCATCTGGTCACGCAGGTGCTGCCCGAGGAGCCGGAGGCGGAGCCGGTCGCGGCGCACCTGGACGAGATCGAGGCGGCGCTCGCCGCCCTGGACGCGGACGATCCGCGCCGCTCCGGGCTGACGCACCGGCTGCGGGTGCTGCTGTGGCGATACGACGAGGGGCAGGGCGCCGGGGACGGTGGGCCCGAGCCTGCCGAGGCGACGGGCGGGGACGACCTGGAAGCGGCGTCCGCGGACGAGATGTTCGCCCTGATCGACCGGGAGTTCGGGGAGTCCTGAGCAGTGGCCGAGCCCGTCGCCCGCAGTTTCCCGCGCCGCCGGGGCGCGCCCTGGTGGACAGTTCCGCTTTAGGCGGTTCGCCGATCATCTACGGAAAGGGACCTGTTGCGGGCGAAAGGGAGAGTTTCGTGATGACCGCGCGACAATTCTGAGGAGGAGTCATGCAGAACACCCCGATCAACTTTTCCCGGCAGGGCCGCCCCCGTCCCTTCGACCCGCCCGATGAGCTCAAGCGCATGCGGGAGGAGCGGCCGATCTCGCCCATGCGATTCGCGGACGGCCATTGGGGCTGGCTGGCCACCGGGCACTCGGCGGTGCGGGCCATTCTGGGCGACGCCCGGTTCAGCGCCCGGCAGGAGCTGGACACCCGGACGTTCATCCCCTCGGCCTCCGAGTTCATCTCCGGCATGGGAATCAGCAGTCCCGGCATGCTCCCGCTGTTGGACCCCCCGGAACACACCCGGTACCGGCGTCTTTTGATGGCCGAGTTCTCCACTCGGCGGATGAAACGGCTTGAGACGGCGATCGCCGCGATCACGCACGGACGTCTCGACGCGATGGAGGCTTCCGGCCCGCCGGCGGACCTCATGGCCGAGTTCGCCGCGCCCATTCCGTATCTGATGATCTGTGAACTGCTGGGCATCCCCGAGGAAGGCCGGGAGGAACTCCGGCAGGCGATCGACACCCTGTTCGCCCTGGACACCGAGCCGAAGAAGGCGGAGTCGGCCCTGACCGTCACGCTCAGCTTCTTCCCGCCGCTCATCGAGCGCCGCCGCGCGGAACCCACCGACGACCTCTTCAGCGGTCTGGTCAACGGCGGTCAGCTGACGGAGGGGGAGCTGGCCACCATCGGCCTCCTCCTGTTCGCGAGCAGCCTCCACACCACCGCGAACACGCTGTCGCTGGGCACCTTCGCCCTGCTGTGCCACCCCGAACAGATGGAGGCGCTGCGCCGCGACCCGGGTCTTGTCGACACCGCGGT GGAAGAGCTCCTGCGCTATGCGACGGTCGGCCAGTTCGGTCCCGTGCGCACGGCATTGGAGGATGTCGAGGTCGAGGGGACGATGATCAGGAAGGGTCAGTCGGTGACGGTGTCGCTGCCGACGGCCAACCGTGACCCGGCGCGCTTCGAGGACCCCGACACCCTCGACATCACCAGGCCCGTCGGCGGCCATGTCACCTTCGGCCACGGCTTCCACCAGTGCCTGGGCCAGCACTTGGCCCGCACCGAGCTGCGGCAGGCCTTCCCGGCGCTGCTGAACCGCTTCCCGGGCCTGCGGCTGGCCGTTCCGCCGGAGCAGGTCCCGACCAGGGAAGACCTGTACCTCAACGGTGTCCATCAGCTGCCGGTCGCGTGGTGAGCGGGCGGGCGGCGCCGCGACGGTGACACCGCCCCGGGAGGGCGCGCGGGTTCGGACGCGCGCGCCCTCCCGGGGCGGCCGCGGTCGGCTGACCGACCAGGGGTGTTCAAGGGGGGATCAAGCGGAGCGGTATTCGGCGGCCAGCCGCTCGAGTTCGGGCACCAGCTGGTTGGGCGACGGCTGGGCCAGTACGTCCTGACGGAGCCGTTCGGCGCCGGACCGCAGCGAAGGGTCTTCCAGCATCCGCGCCAGGTATTTCCCGATTTCCTTGCCCGTCGTGCGGTGGGGCGGAAGCCACTGACCTGCCTGGGCCTCTTCGAGGCGCGCGCCGCGCAGCGCGGCGTCCGGGGTGCTGCGGCTGACCATGAGCTGGGGCAGCCCATGCGCCAGCGAGCTGCAGAAGGTGGGAAATCCACCGTGATGGATCACGGCCGAGCAGGACGAGATGATGGCGTGCAGGGGAACGAACTCCACGAGCCTGGTGTTCCGCGGTACGCGCTCCAGCTCCTTCTGGATTCCGGCCGGCAGGGTCAGGACCAGTTCGATGTCCAGGCCGGCCACGGAGTCCAGCATCTTCTGCAGCTGGGCGCTGGAGAGCAGCGGCTTGGTCATGTCCCACATGCTCGTCCCGAATGTGGCGAGCACCCGGGGCGCCTTCCCCTCATGGTGGACCCAGTCGGGCACGAGGGACCGGCCGCTGTAGGGAGCTCGGCCGTTGTACGGCACATAGCGGGTCGAGAGATGGGGCACACCGGTCTCCAGCCTCATGGAATCCGGCCACTGTTCGATGGTGAACTGCCCGGTCAGCAGCTCTTCGGAGAAATCGAAGCCGTATTTCTCCCCCCATCCGCCGAGCCATTCGCGCACGGGGTCCACCCGCTCCCCCGGGTCCTGCCGCGCCATCGTCTTCAGGAAGTGCCGGCGGAGCCGTACGTCCAGTCCGAGGCCGATCGGCATCCGCGCGTGTGCCGCGCCGACCGCCGAGGCGGCGACCGCGCCCGCGTTGGTCAGCCACTCCCAGACGACCAGATCGGGTTTCCACCAGCGGCAGTACGCGACCAGATCGTCGATCATCGCGTCGTTGACGATCCTGGATCTCGCGACGGCACCGGAGCACAGCGCCTTCAGATAGCTCCACGGAAGCAGCTCTTCGCGGTTCTCGCCCAGATCGATGGGGTCGACGATTCCCTTCCCGTCCCCGAACGCCTCCTTCTGCTCTCGGTACGCCCTTTGCAGGATGGGTTCCTTCGATCCCACCGGGACCGCCGTCAGCCCCGCTTCGGTGACATTGACGGCGAATTCCCGGCTGCTCGCGACATGGACCTCGTGGCCGGCCGTGCGCAGCGCCCAGGCCATCGGCACGGTGAACCAGAAATGAGTTCGCCAGGGAAATGCGACGAACAGGACGCGCATGGGCGCCATCCTCTCTCTCATGGGAGGGACCGCCCTTATTGTCGCCGCCTCCACTAAAGCGGATCTTGCGCACCGGCAGCCTCGTTTTCCGGGCCGGCGCTCCATGTCAGTCCGCCTTTAGCGGCGTACGGGAAGCTGCGTACCGGCAGTCACCCGAGCAACATCGACGAGTGGGAGAGCGAGATGGTGCAGGGTTTCCAGGCCAGTCGGCAGTGGGAGCGCATCAGCGAACTCTGGGTCACCGAGGACGCGGCCACCGATCTGACCGGTTTCAAGTCGGATCGGCGGAATTTCAATATCGCGCTCTGGGATCCGACGGCCAATGGGGTCAGGTATTTGAGGGCCCTTGTGTACGAGCTGGCCACGCGGCTGAGCGAAGACGACTGGTCGAAGATCGAGAATGTCCGCAACCGTGAGGTGGGAGACCCCGTCACGGTCCGCTACGAGGGCAGGAGCATCTGTCTGGACTATCTCCAGGCGGCGCTCGAGCTCGGATTCATCGAGAAGGAGCTGGACCTGGGCGGCGCCCGTGTCCTGGAAATCGGCGCGGGGTACGGCCGCACCTGCCACGCGATGCTGTCCAACTACGACCTCGCCTCCTACACCATCATCGATCTGAAGAACACGCTCGCGCTGAGCAGGGCGTATCTGCGCGCCGTCCTGGATGAGAAGCAGTTCGCCAAGATGAAGTTCGTGCAGGTGGAGGACATCGACACCGCGCTGGGCGCGGACCGCTTCGAGCTGTGCGTCAATGTGCACTCCTTCACGGAGATGGCCCCCGACACCGTCGCCGCCTATCTGCGTCTGATCGACGAGAGGTGCGGGGCGTTCTTCGTGAAGAACCCGGTCGGGAAGTTCCGGGACAAGAGCATGGACGGCCACCAGAAGGGCGAGGAGGCCGTGCGGCTGGCCATGGAGAGCGGACCGCTGCGGCAGGTGCTCGACATCCACGACAGCGAGGCCGTGGCGGCGGCCGTGCCCGCCTTCGTCGACGCGTACCGGCCCGGCGACAAATGGACCTGCGCCGCCGATACGCGGGGCCTTCCCTGGAGCTACTTCTGGCAGGCTGTCTTCCGGAAAACAGGCGGCGAGAAGACCGGCAGCGACCTTCCGTAGCCCCGTCCTCCCGGGAGGGAACCATGGCCGCCTCTCCGGCGCCCGCGCTCCGCGCTGACGACGCTCCGGCCGGCACGCCGGTCCTGGTGCTGGGCGGCTCCGGATACCTGGGGCGGCACATCTGCGCGGCGTTCGCCGCCACGGGCGCCCGGGTGTCACGGGTCTGCCGCGGCACGCGCGGTGGCGGCGCGGACGGGGACGGCGCGGACGGGGACGACTGCGGCTCCATACGCCTGGACCTGAGCGCGGCCGGCCCCGCCGAGCTGGCCCGGCTGTGCTCCGACACGCGGGCGCGGGTGGTGGTGAACGCCTCGGGCGCGGTCTGGGGCGGCGGCGAACGGCAGATGACGGAGGCCAACTCCGCGTTCGTGTCCCGGCTCGCCACCGTCGTCGCCGGGCTGCCGGACCGGCCGCGGCTGATCCATCTGGGCAGCGCCTACGAGTACGGCCCGGCCGCGCCCGGGACCTCGATCGGCGAGGACTGGCCGACCGACCCGGCCACCGTGTACGGCCGCACCAAACTGCGCGGCTCGCAGGCCGTGCTGCGGGCCGCGGCCGAGCGCGGCGTGCCCGGGACCGTGCTGCGCATCTCGGTCGCCTGCGGTCCTGGCGCCCCGGGCAACAGCCTCGCCGGGGTCG TGGCCGCCCATCTGGCCTCCGGGCGCGGCGAGTTGCGGCTGGCGCCGCTGCGCGACCACCGGGACCTGGTGGACGTCCGGGACGTGGCCGACGCGGTGGTCGCGGCGGCCGTGGCGCCGGTGCGCGCGGTCGCCGGAACCGTCGTCAACATCGGCAGCGGCCAGGCGGTGCCCGTGCGCCGGCTGGTCGACCTGATGATCTCGCTCAGCGGGCTGCCGGTGCGCGTCGTCGAGGACCCGGCCCTGCAGCGGACGCGCTCCGACGCGGCCTGGCAGCGACTGGACATCGGACGGGCGCGGCGCCTGCTGGGCTGGGCGCCGCGCCGAACCCTGCGCGAGTCACTGCGTGATCTGCTCGCGGCCGCCGGCTCGCCGCCCGCGGCGGCGCGTACGGCGACGGCATGCGGGCCCGGGAACAGCCATGGGAAGGACAGCAGATGAGCGAACGGGTCGCGCGCATCCTCGACGAGGTGCGCAAGTACCACCAGGACACTCAGGAGGGGCGGGGCTTCATCCCCGGGGTCACCGAGATCTGGCCCTCGGGTGCGGTGCTGGACGAGGAGGACCGGGTCGCGCTGGTGTCGGCCGCGCTGGAGATGCGGATCGCGGCGGGCGCGTTATCCCGGAAGTTCGAGTCGGCCTTCGCGCGCAGGATGAAGCGGCGCAAGGCCCATCTGACGAACTCCGGGTCGTCCGCGAACCTCCTGGCGCTCTCCTCGCTCACCTCCCATCTGCTGGGCGACCGCCGGCTGCGCCCCGGCGACGAGGTCATCACGGTGGCGGCGAGCTTCCCGACCACCGTCAACCCGATCCTGCAGAACGGGCTGGTCCCCGTCTATGTGGACGTCGAGCTGGGGACGTACAACGCCACGGCCGAGCGGGTGGCGCAGGCGATCGGGCCGCGCACCCGGGCCATCATGATGGCCCATACGCTGGGCAACCCCTTCGAGGCCGCGGAGCTGGCCCAGCTCGCGCAGGACCACGATCTGGTCCTGATCGAGGACACCTGCGACGCCGTGGGCTCCACCTACGACGGGCAGCTCGCCGGCACCTTCGGCGATCTGACGACCGTCAGCTTCTATCCCGCACACCATCTGACGATGGGCGAGGGCGGCTGCGTGCTGACCTCGAACCTGGCGCTGGCGCGGATCGTGGAGTCGCTGCGGGACTGGGGCCGGGACTGCTGGTGCGAGCCGGGGGAGAGCGATACGTGCCGCAAGCGGTTCGGCTACCAGATGGGCACGCTGCCGGCCGGCTACGACCACAAGTACATCTTCTCCCACATCGGATACAACCTGAAGTCGACGGATCTGCAGGCGGCGCTGGGGCTGGCCCAGCTCGACAAGCTCGACGCATTCTGCTCGGCACGGCGGCACAACTGGCGCCGGCTGCGCGAGGGTCTTGACGGGCTGCCGTGGCTGATCCTGCCGGAGGCGACGCCGCGCTCCGATCCGAGCTGGTTCGGGTTCGTCCTGACCGTCGACCCGCGGGCCCCGTTCAGCCGCGCCGAGCTGGTCGACTTCCTGGAGTCCCGCAAGATCGGCACCCGTCGGCTGTTCGCCGGGAACCTGACCCGTCACCCCGCGCACGCCGAGGCGCCGCACCGGGTGTCCGGCGAGCTGACCAACAGCGACATCATCACCGAGCACACGTTCTGGGTCGGCGTCTACCCTGGGCTGACCGAGGAGATGATCGACTACATGGTCTCGTCCATCACCGAGTTCGTCGGGGACCGCGGCTGAGCGGCGCCACGGCACGCCGATGGCCGCGCGGGCGCCGCGCGGTCATCGGCGTATCGGCTGGGCGGAGTGCTTGAGCGGTTCCCACCAGCCGCGGTTGTCCCGGTACCAGGCGACCGTCTCGGCCAGCCCGGCGGCGAAGTCGTGGCGCGGGCGGTAGCCGAGTTCGGCCCTGGCCTTGTCGTCCCGGATCGCGTAGCGCCGGTCGTGTCCCTTGCGGTCCGCGACGTACCGTACGCGGCCCCAGTCGGCGCCGCAGGCCTCCAGCAGCCGGCCGGTCAGCTCCCGGTTGGTCAGCGCGGTGCCGCCGCCGATGTTGTAGATCTCGCCGGGGCGCCCCTTGGCGCGCACCAGTTCCACGCCGCGGCAGTGGTCCTCCACGTGCAGCCAGTCGCGCACGTTCAGCCCGTCGCCGTACAGCGGCACGTCCTCGCCGTCGAGCAGGTTGGTGATGAACAGCGGGATGATCTTTTCGGGGAACTGGTGGGGACCGTAGTTGTTGCTGCACCGCGTGACGCGCACGTCCAGGCCATGGGTGCGGTGGTAGGCGAGGGCGATCAGGTCCGACGCGGCCTTGGAGGCGGCGTAGGGCGAGTTGGGGTCCAGTACGGCGGTCTCGGGGCTGGAGCCGGTCTCGACGGAGCCGTAGACCTCGTCCGTGGACACGTGGACGAAGCGCTCCACGCGGTGCCGCAGGGCCGCCTGCAGCAGCGTGTGGGTGCCCTGGACGTTGGTGCGCACGAACGCGTCGGCGCCGTCGATGGAGCGGTCGACGTGGGACTCGGCGGCGAAGTGGACCACCTGCCCGGACCGCGCCATCAGGGTGTCGACCAGCTCGGCGTCGCAGATGTCGCCGTGGACGAACGTCAGCTGTGGGTCGGCGAGGTCGAGGTTGCGCCGCGTCCCGGCGTAGGTGAGCTTGTCCAGCACCGTGACATGGGAGACCTCGGGGCCCCACGGGTCACCGTCCCGCGCGAGGAGCCCGCGTACGTAGGTGGAGCCGATGAACCCCGCGGCCCCGGTGACGAGGAGTCTCATATTTCCTCCAGGAACCGCGCGGGCGGCAGGCCCTGCCGGTACGAGCGGGCCCGGTCCATGACGTACTGGCCGTAGGGCGAATTGCTCATCTCCGCGCCGAGCGCATGACAGTCGTCGGGTCCGATGAATCCCATGTACATCGCGATCTCCTCGACACAGCCGAGCCGGGTGCCCTGGTAGTGCTGTACGTCGCGCACCATGCGGCCCGCGTCCAGGAGGGTTTCGTGGGTGCCGGTGTCCAGCCAGGTGACGCCCCGGCCGAGCCACACCAGGTCGGCCCTGCCCTCCTCCAGATAGGCCCGCAGAACGTCGGTGATCTCCAGTTCGCCCCGGGCCGAGGGGACCAGGCGCTTGGCGATGTCGACGACGCTGCCGTCGAAGAAGTACAGGCCGGGAATGGCCAGGTTGGACCGGGGCCGCTCCGGCTTCTCCTCGATGGACAGCAGCTCGCCCCGGTCGTCGATCTCGGCGACGCCGAAGTGCCGGGGATCGGCGACCTCGTGCCCGAACAGCACACATCCGCGCAGCCGCTGGGCGCTCCTGCGGAGCAGCGCGGGCAGGTTCGCGCCGTGGAAGAGGTTGTCGCCCAGGATCAGGGCGCACTCCTCACCGCGAATGTGATCCGCGCCGACCCGGAAGGCGTCGGCGATTCCCCGCGGTTTCTCCTGGGAGGCGTACGAGATGTTCAGCCCCAGATGGCCGCCGTCCCCGAAAAGCCGGCGGAACTGCGGCAATTCGGAGGGGCGGCTGATGATCAGAATGTCCCGG ATACCGGCGAACATGAGGACGGACAGCGGATAGTAGATCATTGGCTTGTCGTAAACCGGAACCAACTGTTTGGACCCCGCCAGGGTGAGCGGTTGCAGCCGTGTTCCGTTCCCGCCCGCGAGAACGATGCCTTTCATCATATGCGACATCGTCACCCACCGGTCTAAAGCATGACTAACCGCGAATGGGCGCCGGCGCGGGCTCGCAAGAACCGCTTTAGAGGTGTCGGGACGATAGCGGGTGAGAGATGCGGCGTGGTCGACGGCGAGGAGATGGCAGCCATGCGGGTCCTGTTCGTCACTTTTCCCGGGCGGAGCCACTTCCAGCTCAATGTGCCGTTGGCCTGGGCACTGCGGACCGCGGGACATGAGGTTCATGTCGCGAGCGCCCCCGAATTCGCCGACGTCGTCACCGGAGCGGGGCTGGCCGCGGTCGCTGTCCAGCCGAGCGCCGAGGACCTGGTGGAATACTGCCGCTGGTGGCGGCCCGATCTGGTGATCGGGGAATCGGCGAGTCAGGCGGGCGCGGTCGCCGCCGCCGCGGTGGGCGCACCGCACGCACGGATGCGACCGGTCTTCGGCGAGGAATACGGCGACCCGCCGGAGGAGCCGCTGACGGGCCGTTTCACCATCGATCAGACACCGGGATCCCTGCGGCCCGAGAGCGGTCCAAGGACCGTGTCCGTGCGCTACGTCCCGTACGACGGCCCTTCCGTCGTACCGGAGTGGTCCCGCCGCGATGCGGAGCTGCCGCGCGTCCTCGCGGCCTTCGGGGTGAGCAAGTGGAACGGGGGCCGGCCCGTTTCCGCCGAGGAGGCCCAGGAGATGCTGGACGCGACGGCCGACCTCGACATCGAGCTGGTGCTGACGCTCCCCGCGGGAATTCGGCGGACGCTCCAGCGCGTGCCCGCGCACGCGAGAATCGTCGACTTCGCACCGCTGCACGTCCTCCTCCCGTCCTGCTCGGCGGTGGTGCACCGCGGGGGTCTCCCGGCCTTCTGCGGCGCGCCGGCGTACGGAGTTCCGCAGCTCATGGTCGGCCCCGGCGGTCCGGAATCCCGGGCCCGGGGCGGGCGTCTTGCGGAGGTCCGGGCCGGGCTGTGGATTCCGCCCGAGGCCATGAGCGGGCCGAGGTTCCGGGAGGATCTGGTCCGGCTGCTGGACGATCCGTCGTTCCGGTCCGGGGCGGAAGGGCTGCGTCAGGAGATGCTGGCTCAGCCGTCCCCCAATGACGTGGTTCCCGAACTGGAGAAGCGGGTGGCCGAGTACGGCTCCCGTTGACGCATCGACGCTGTTTTCCCCGAATTCCCCCGAGGGGTTCGCCGGACCGCCGGCGAACCCCTCTTTTCTTCGGCCCTTCTTCGGGCCCCGCTCAGGACTTTCCGAATTCGGTGGTGATCAGGTCGAAGATCTCCTCCGCCGTGGCCGTCTCCAGATCGTGGTGGGACCCGGCGGCGCCGGCCGTACGGGCCGGGTCTGCCCACTTGTCGGCCAGCAGCCGCAGCCTGGCGGCGATCCGGGCGCGGGCGGCCTCGTCCACGGCGTCCGGCGCGTGGCCGGCGTCCCAGCCGTCGAGTTCCGACATGAGCTGCTGTTCGCCCGAGGCCGCCTCGGCGCCGAGCAGCCCGTGCAGATGACGGGCCAGTTCCTCGGCCGATGGGTAGTCGAACAGGACCGTCGTGGGCAGTCGCAGCCCGGTGCCGGCGTTGAGCCGGTCGCGCAGCTGGACGGCGGTGATCGAGTCGAAGCCCAGCTCCTGGAAGGGCTTGGTGGGCGGGACGGCGTCCGCGTCGTCGTGCCCCAGGGTGCTGGCCGCGTGCGCCTGCACATGGCGTACCAGGATGTGGTGGCGCTCGGCCGGCGGACTGCCGGCGAGCTTCCGCCGGAGCGGGTCCGCCTCCTCCTCCGACGCCCGGGACGGCTCCTCGGTCCGCGCCGTCGCGGCCTCGGGGAGGTCGGACAGGAGGGGGCTGGGGCGCAGCGCGGTGAAGCTCGCCAGGAAGCGGCGCCAGTCGACGGCGGCGATCACGGCGCCGGCCTCCCGGCGCTCGACCGCCTGCTGGAGCGAGGCGACGGCCAGGTCGGGGTCGAGGGGGTGGATACCGCGGCGGCGGAAGAAGTCGATCTCCTCGGCGGAGGTGGCCATGCCCACCGCGCCCCACAGCCCCCAGGCCACGGAGGTGGCGGGCAGGCCGAGGCCGCGGCGGTGTTCGGCCAGGGCGTCCAGGTAGGCGTTGGCGGCGGCGTAGGCAGCCTGCCCGCCGCTGCCCCAGGACGCGGTGACCGAGGAGAACATGATGAACGCCGAGAGGTCCAGGTGCTGGGTCAGCTCGTGCAGGTGCCGGGCCGCGAGCGCCTTCGGGGCGAGCATACGGTCCAGATGCGCGTCGTCCACGTCCGCGATGAGCTCCTGCTCGCTGACGCCGGCGGCGTGGATGACGGTGGACAGCGGGTGCTCGTCGGGGATCGCCTCCAGGACGCAGCGCATGGCCTCGCGGTCGGCGGCGTCGCAGGCGGCGACGGTCACCGTGGCGCCCAGGGCGCGGAGTTCGGCGGCGAGTTCCCCGGCGCCGGGGGCGTCGGGGCCGCGGCGGCTGGTGAGCAGCAGATGGGCCGCCCCGTGTCCGGCCGCCCAGCGGGCCAGCAGCGCGCCGACCCCTCCGGTGCCACCGGTGATCAGCACGCTGCCCCGCGGCTGCCACGGCCGGCGCGCGGTGGAGCGGTCCGAGGTGGCGTGCACGATGCGGCGCGCCAGCACGCCGGTGGTACGGATCGCCGTCTGGTCCTCGCCCCGGCCATGCCCCGGGGCGCCCGACAGCAGGGCCGCCAGCCGGGGCGCGGTCCGCTCGTCGGGGTCGGCGGGCAGGTCCACGAGGCCGCCCCACCGGTCGGGGTGTTCCAGCGCGGCCACGAGGCCGAAGGCCCAGGTCTGCGCCTGGAGCGGATGCGTCAGCGGGTCGTCGTGGCCGGTGGACACCGCGCCCTGGGTCAGGCACCACAGCGGCGCGGTGATCTTCGCGTCGCCGAGTGCCTGGACGGCGGCGACGGTGGCGGCGAGCCCGGCCGGCAGGGCCGGATGTGCGGGGTGCGGGCGCTCGTCGAGGGCGAGCAGGCTGAGCACCCCGGCGAGGGGGGTGTCGGGGCCTTGGGCGGCCAGCCGTTCGGCGAGCGCGTGGCGGCCGGCCGCCACGGGGTCGAGGGGCCATGCCGTGGCGTCGGCGCCGTGCTCGGCCAGGGCCCGGGTCGCGGTCCGCACCGCCGGGTGGTCCTCGTGGCCGGCGGGGACGAGTACCAGCCAGGTGCCGCTCAGCGCGGGCGGCTCGGGCTCGGACAGCGGTGTCCACCGGACCCGGTGGCGCCAGGAGGCCAGCGTGACGCGTTCGCGGTGGCGGCGGCGCCAGGCGGACAGCACGGGCAGGGCGGGCCGCAGCGCCTCGATGTCCCCGGGGGCGGTGGCGTCGCCGTCCGGCTCCAGCACCCGGCCCAGCGCCTCCATGTCGCGGTCCTCGATGGCGTCCCAGAGTTCGGCCTCG ACCGGGTCCCGGGTGTCGTCGCCCAGGGCCGCACGGGCCGGGGGCAGCCAGTACCGCTCGCGGTCGAACGCATAGGTGGGCAGGGACACCGTCGCGGGTGCGGACGGGCCGGCGAACCAGGGCCGCCAGTCGACGGGCGCCCCGGCGGTGAACGCCTGCGCCAGCGCGGTGGCCAGCTGCGCCGGGCCGCCCTCCTGGCGGCGCAGCGTGCCCAGCGCCACCGCGTCGACGGGCGATCCGGCAGTTGCTGACTCGAAGCACTGCTGGAGCGCGGGGACGACCACGGGGTGCGTGCTGACCTCGATGAACAGCCGGTGTCCCGCGGCGAGCAGGGTGTCGACCGCCTCGGTGAGGCGGACGGGCTGCCGCAGGTTGGTCAGCCAGTAGTCCGCGTCCAGGGCGGTGGTGTCCATCAGGCCGCCGGTCACGGTCGAGCAGAACGCGACGTCCGTGGCCGCGCCGCCGACCCCGGCGAGGCGGGCCGTCACCTCGTCGGCGATCTCGTCCACCTGGGGGCCGTGGGAGGCGTAGTCCACGTCGATGGCGCGGGCCCGGGCGCCGGTGGCCTCGCAGGCGGCGACGACGGCGGCCACCGCGTCGGGCGGGCCGGAGACCACGGTGGAGGACGGGCTGTTGAGCGCCGCCACGCCCACGCCCGAGGCTGCCTCGCCGAGGTCGGAGACGATGCGCTCGGCCGCCTCGGGGGCCACGCCGAGGGAGGCCATCGCGCCCCGGCCGGCCAGCGCCCGCAGCGCCCGCGAGCGCAGCGCCACCACCCGGGCGCCCTCCTGGAGGGTGAGCGCGCCCGCGACGCACGCGGCGGCGATCTCGCCCTGGGAGTGTCCGACGACGGCCGCGGGCCGCACGCCGTGGTGGGACCACAGGGCGGCCAGGGAGACCATCAGGGCCCACAGCACCGGCTGGACCACATCGACGCGGCCGAGGTCGGCCGCGCCGTCGGCCCCGCGCAGCACATCCGTCAGGGACCAGTCCACGTACGGGGCGAGCGCCGCCTCGCACTCGGCGATCCGGGCGGCGAACACCGGTGAGGCGCCGAGCAGTTCCGCGCCCATCCCGGGCCACTGGGAGCCCTGCCCGGGGAACACCAGGACCGCTCCCGCGCCCGCCTGGCCCGGTGCTCCGGTCACCACTCCGGGCGCCGGCTCGTCCGCCGCCAGCGCGCCGAGCCGCGCCCGCAGTGTCTCGGTGTCCGGGCCGGTGACGACCGCTCGGTGCTCGAACACCGAGCGCGTGGTCACCAGCGACCAGCCGACGGCCCGCGGATCGGCGTCGAGCGTCGCGTCGAGCAGCCGCCGGGCCTGTGCGCGCAGCGCGGACGCGCTGCGCGCCGACAGCACCCAGGGGGTGACGGGCGCGGGCTCGGACGCGGACGGCGCGGGCTCGGACGCGGACGGCGCGGGCTCGGGTGCCGGAGGCCCGGGCTCGGGGGCCTCTTCCAGGATCAGGTGCGCGTTGGTCCCCGAGAAGCCGAAGGCGGACACCCCCGCCCGCCGGGGCCGCGCGGCGCGCGGCCACTCGACGGCCTCGTCCAGCAGCGCCACCGCGCCGGAGTCCCACGCCACATGCGGGCTCGGCTCCGCGATGTGCAAAGAGCGCGGCAGCGTCTCGTGGCGCAGCGCCATGACCATCTTGATCACACCCGCGGCGCCGGCGGTGATCTGTGTGTGCCCGATGTTGGACTTGATCGACCCCAGCCACAGCGGCCGGCCCTCCGGCCGGTCCTGGCCGTAGGTGGCCAGCAGCGCCTGGGCCTCGATGGGATCGCCGAGGCTGGTGCCGGTCCCGTGGGCCTCCACCGCGTCCACGTCGGCCGCGGAGAGCCGGGCGTTGGCCAGCGCCTGCTGGATGACGCGCTGCTGCGCCGGGCCGTTGGGCGCCGTCAGCCCGTTGCTCGCCCCGTCCTCGTTGACGGCGGAGCCGCGGATGACCGCCAGGACCCGGTGCCCGTTGCGGCGGGCGTCCGACAGCCGCTCCAGCAGGACGATCCCCGCCCCCTCGGCGAGGTTCATGCCGTCGGCGTCCGCCGAGAAGGGCTTGGCGCGTCCGTCGGGGGCCAGGGCGCGCAGTTCGCTGAACCCGACCAGCGGCGCGGGCGAGGACATGATGTAGACGCCGCCGGCCAGCGCCAGCGTCACCTCCCCCTCCCGCAGCGCCTGGCAGGCCAGGTGCATCGACACCAGCGCCGAGGAGCAGGCGGTGTCCAGGGTGACCGCGGCGCCCTCCAGGCCGAGGGCGTACGCGACGCGCCCGGCGACCACGCTCGGGGAGTTGCCGGTGGTCAGATAGCCGGCCGCGTTCTCCGGTACGTGGTAGGAGTTGAGGAAGTAGTCGAGGCCGTCGCAGCCGAGGTAGGTGCCGGTGGCGCTGGAGCGCAGGGTGTGCGGGTCGATGCGGGCGTGTTCGATGGCCTCCCAGGCCACCTCGAGCGCCAGCCGCTGCTGCGGGGCCATGGTGACGGCCTCGTTGGGGCTGATCCCGAAGAACGCGGCGTCGAAGCGGTCGGCGTCGTGGACGAAGCCGCCTTGGCGCACATAGCTGGTGCCCGGATGCCCGGGGTCGGGGTCGTAGAGCCCGTCGAGGTCCCAGCCGCGGCCGGTGGGCAGGCCGCCGGTGCCCTCGCGGCCGGCGGCGACCAGGTCCCACAGGTCCTGGGGGGAGTTCACGTCACCGGGGAACCGACAGCCGATGCCGATGATCGCGACGGGCTCGTGGTGGGCGTACTCGACGTCCTTGAGCCGGCGCTGCGCCTCGCGCAGATCGGCCGTCACGCGCTTGAGGTAGTCGAGGAGCTTCCTGTCGTCGTCTGCCATGTCAGGACCGCTCCAGGGCCGACCGGTCCGCGTTGCGGGCCAGTTCGAGCAGGTCGGCGGCCACCCGCTCGGGCTGGTCCGCGTGGAGGTTGTGGTCGGCGCCGACGTACCAGCGGGAGTCCACGTGTGCGATGGCCGACTCGGCCTCGGTCACCCACTTGCGCACCCAGTCCCCGAGCGCGGAGGCGTACGAGGGCAGGGCTATGAGCATGAGGACGGGCACCGCGATGCCGGGGTACCAGCGGGCGGGCGAGTCGTTCCACATGCTGTTGAGGATCGACAGGCGGTGTTTCAGCGGCAGCCGCTGCACGAGCAGCCCGTCCGGCCCTTCCACCGTGTCGGCGAGCATCGCCTCGACGGCGTCCTTCGACCAGTGCGGGTGGAGCGTTCGTATGCGCTCCCGCGCGGTGTGAACGGTGACTCCCGTGGTGCCGGGGACCCCGTTCGCGATCTTCTCCACGTCCTTCTGCCAGGTGGGGATCCGCAGCGGGGTGTCGGTGTGGTTGATCCACCCTCCGTCCACCAGCGCGAGGCCGGCGGCCAGGTCGGGGTGGACGGAGGCGAACCGCACGGCGACCATCCCGCCCCAGGAGTGGCCGGCGAGCAGTGCGCCCGTCAGCCGCAGCTCGCGGCAGACGGCCGCCAGATCCGCCACGGCGGCCTCGTTGTCGTACCCCTGCTCCGGTACGTCGGAG TCGCCGTGTCCGCGCGTGTCGATCGCGTAGACGGGGTGGCCGGCGTCCGCGAGCCGGCGGGCCACGTCGTCCCACATCCTGGCGTTGGACGCCAGCCCGTGGAGCAGCAGGAACGGCCGGCCGCCGCGCCCCGGCCGGTGCCGCACGTTCAACGTCACTTCGTGGTCAACCGGAACGGACAGCCTCATGACGGGGTTCCCCTGGCTCCTGGGAGTTGGGACGAGATGGTGATCGGCCGGGCGGGTCGCGGCGCCGGCGCCTAGGCGGCGTCGGCCGCGTAGGCCTCGGCGAGGTACTTGCCGAGCAGGGTGGAGGTGGGGTGTTCGACGATGGACACGAGGGGGACCTCGATCCCGGTGTCGTCCATCAGGAACTTCGAGAGCTTCACGGCGGTGAGCGAGCTCAGGCCGTTCTCCAGGAAGTTGCTCTCCTCGTCGAGCGACGGTACGTCGAGGATCTCGGCGGCCCGGCGGAGCACCAGCTCGCACAGCAGCTGCTGGCGTTCCTCCGGCGCCGCCGACGCGAGCCGATCGGCCAGCTGACCGTCGTCGGGGGATCCGTTGGTGGGTTCCATGGGTGCACCCTTCTCGGTGTGGTGTCGGGCTGACGGGTGTGTGCGGTGGGCTAGACGGTGGGCCGGCCGAGTACGCCTCTTGGGGCCGTGCCGGACGGCTTGAGCCCGACCATCTCCAGCTCCTCGGGCCGGAACGGCGGTTCCACGAGCTCCGGGTAGCTGGTGTCGGACTGCAGCAGCGACAGGAAGCGGGCGTCGCCCAGGGCCTTCTGCGGCGCGTTCAGGCTCAGCACGCCGGTGGCGACCTCGCAGACCTTCGGCGACCTGATCGACTTGCTGGAGATGTAGTCGGAGAACCTCAGCCGCTCCGCGGTGTCCGGGCCGGTCAGCCGCGGGTCGCGGGACAGGTTGCGGCAGTTGACGAACTGGATGTCGTTCAGCCCCGCCATGATCCACGGGTCGTCGACCACCTCGCTCAGGGCCCGCTGGATCTGCCGGCCGCGGTTCTCGCCGAACCCCTCGCGCGCCAGTTCCCGGTCCAGGGCCTCGGCGGCGCGGGCGGCCGAGCTCATGCCGTGTCCGTAGACCGGGTTGAACGCCGCCAGGGAGTCGCCGAGGATCAGCAGGCCCTCCGGCCACTGCGCCATGCGCTCGGGGTACAGCCGGCGGTTGGCCCCCGAGTGCGACTGGAAGATCTGGCTGATGGGTTCGGCGACGCCGATGAGGTCGCTGACGATCGGGTGCCGCAGCTCCTTGGCGTAGGCCGCGAACTCGTCCTCGCCGCCGGGCAGCGGAGCGCCGCGCGTGCTGGTCAGGGTGACCAGCCAGCGTCCGCCCTCCTGGGGGTAGACCACGCCGAAGCGGCCGGGCTGCCGGGTGAGCGGGTCGGCGGCGACCTGGATCGCGGGGAAGGCGCTGCCCGCGCCCTCGGGCGCCTTGAACATCCGGGTCGCGTACCCGATGCCGGCGTCGACCACGTCCTGCTCGACGGTCGGCAGGCCGAGGGCGGCCAGCCAGCGGCCCAGGCGCGAGGCCCGCCCGGTGGCGTCCACCACCAGGTCCGCCTCGATCAGCCTGGGTTCGCCACCGTCCGCGTCGCGCACCCGCACCCCGGTGACCCGGGAGCGGTCGCCGGCCAGGTCGACGGCCTCGGTGCCCTGCTCCACCCGGACCCGGCCGTCGGCGAGGATCGCCTCGCGCACCGTCCAGTCGAGCAGCGGGCGGGTGCACATCATCGCGTACGCGGTGGCCGGGAAGCGGTACTGCCAGCCCGCCGAGGTCAGGGTCACCAGGTCGCTGTGGAAGCCGATCCGGCGCGCACCGGCGGCCAGCAGCCGGTCGGTGGTGCCCGGCAGCAGGTTCTCGACGATGCCCGCGCCGCTGGACCACAGCACGTGGACATGGCGGGCCTGTGGCTGTCCCTTACGGTGGTGCGGCCCGTCGGGCAGGATGTCACGCTCCACCACGGTCACGGTTGCCAGGTGACGGGACAGCACATGGGCGGCCAGCATGCCGGCCCATCCGCCGCCCAGCACAACGGCGCGTGTCGGTGTGGTCATGGAATCAAGTCCCTTCGCAACGGGCTCGTCCGCGCGGGTGTCTCTCTGCGGCCTGCCAGGCCCATCACGAGGGTGTCCTGAACAGCTTAAGCGGGCCTTTCGGCTCCGGCGGGTCCGTGGCGCGGGGCGCGGGGCCGGCGCCCGTCCCGGGCTCGGAGTGCCCCAACGTGGTGTCCGTGAGGCCCCAGAACCCGCGGATGTGAACGCCCAGGCCGTCCTCGTCCAGTTCGCAGATGGTGATCATGCGCATGCGTATCTCGGCCGGTACAAACACCCGCGCGTCTCCGCGGATCACCACGAACCGTCCGTCCATCGACGACACGGGCCGCCCCAGCACCTCGTGCACCTTGCCCTCCGAGCCCCGGAACACGTGCTCGCGCAGGGCGTCCTTGCCGCGCAGCAGCAGGCCGCCCACCGGGTCCTCGAAGACGATGTCCTCCGCGAACATGGCGACGGTGCCTTCGATGTCCCCCTTCGCCAGCAGTCCGAGGTAGGTGCTCGGCAACCGCCTGAGGGCCGCCTCCTTCGGCGCGATGACGTCGGGGCCGCGGAACGTCCCGGGCGCGCCCGCCGCCGAGACCTCGAGGTCGGGCCGGCCCCAGTACGCCCGCATGTCCTCGATGAGTCCGGACTCGCCGACGCGGAGCAGCAGCGCGTAGTGGCAGCGCAGGCCGGCCGGTTCCGCGCCCTGGGGCGGGGGCAACCAGCCGCGGTCCGCGTACACCGGGCCGCGGGGCCGGTAGTCCATGTCGGCGGTGATCCTGGACAGGACGTGTGTGCCGTCCTGACCGACGACCGTGTCCTCCACGGTCTCCCGTACGTTTCCGGCGACGGCGCTTCGGAAGTGTGCGCGCAGCGCCTCGTGTCCCCTCAGGCCACCGGTCCCGACGGGGTCCTCGAACGTGGCGTCCGGCGCGTACAGGCCGATCAGCGCGTCGATGTCGCCGGCGCCGAGGAGCCGGCTGTGTTCGAGTGCGATCTTCTTGCGGGTGCGCTCGTTGAGCACCGTGTCCTCCCTGGCCTTAAGGACCTCGGCCAGTCACCCTTGCCGAACGGCCTTAAGCAGCCCTTTCCGCCGGTGCGCGGGCGGGCGGGGCCCGCCCGGGCGACAGGCCTGCTTTAGGCGGATCGGCGATCCTCCGCTGAGACCGGTGCGGATGGCCGACGACGGAAGGCCCGAAGATGACACCAACGAAGACCGAGCAGGTCATCATCGACAACGGCGGCAACTCGGTGGTGAACGGGTTCCGGTCCGCCGCGCGCTTCGTGCAGTGGATGACCGCCTCCCCCACCTCCCGCGTCAGCGCGCTGTACGAGCTCAACAGCCGCAGGCTCACCGGACGCAGCGCCTACCGCGGCTTCCTGAATGTGACGCGCAGCCAGGTCGAGATCGGGCGGCGGCGCGCCGCGGAGAAGAACCTGAGCGGCCGGGTGGACCTGCGCCTGGGCTCGGCGACGGACGTCCCC TTCGAGGCAGGTTCCTTCGACCGGGTGGTCGCCCTGGAGTCCGCGTGTCATTTCGACACCCGTCAGAAGTTCTTCGAGGAGGCGTTCCGGGTGCTGCGGCCCGGCGGTGTGCTCGCCACGGCCGACCTGGTGCCGCGCCCCGCGCCCCGCAGGAAGACCCTGGCGGCCCGCTTGGAGCGGTTCGGGTATGAAGGCCTACATCAAAACGATCACCGGGCAGGCGGTACGCCTGGAGCACATCATCGCGGCGGCGCGGAAGCCCGGTGAGGCGTGATGCCCACAGGCGAGACGCCGAAGACGCCGGTGCGGTGCCCGGTCTCGGTCAACGACCCGGAGCTGACCGCCGACCCCTACCGCGGCTTCAGCCGCATCCGGGAGGAGGCGCCGGTCACCCTGGGCACCCTGGCCGACGGCCGGAACATCTGGGTCGTCACCCGCTACGACGACGTCACGGGCGTGCTGACCGACAGCCGCTTCGTCAGCAACTCCCGGTCGGTGCCCGGCTCCACCACGGACCGCCACGCCGCGTCGCTGACCTGGCTCGGCCTGCCCGAGGAGCTGATTCCCTATCTCTCCCGCGCCATCACACAGCTCGACCCGCCCGACCACACCCGGCTGCGCAAGCTCGTCACCCGGGTGTTCTCGGCGCACCGGGTCGCCGCTCTGCGGCCGCGGATCCGGACGCTGACGCGGGAGCTCGTGGACGCGCTGCCGGGACACGCGGTGGACGGCACGGTCGACCTGGTCGAACACTTCGCCCAGCCCCTGCCCGTCACGATCATCTGTGAGCTGATCGGCGTGCCGGCCTGTGACCGGGCGATGTGGCTGCGGTGGAGCCACGACTACAGCGACGCCCTGCGGCTGAAGAACACGCTCATCCAGGCCACGGCCTATCTGCGGGAGCTGGTCGCCAAGCGCCGGGTAGAACCGGCGGACGACCTGCTCACGGCGCTCATCCAGGCCCGTGGCGAGGACGGCGAGGGCCTTTCGGAGACCGAGGTGGTCACGATGGCCTTCCACCTCGTGGTCTCCGGCCACGGAACCACCTCCGGCGTCATCGGCAACAGCGTCCTCGCCCTGCTGACCCACCCCGATCAGCTCGCCCTCCTCCGGAGCGATCCGGAGCTGATCCCGGGAGCCGTCCAGGAGCTGCTGCGCTGGCGCAGCTCGGTCGTCTTCGGCAAGGCGCGGTACGCCACGGAGGACGTCCGGGTCGCGGACACGCTGATCAGCCGGGGCGATGTCGTCCTGCCGGTGCAGGGCGCCGCCAACCACGATCCGCGGCACTTCCCCGACCCTGAGCGGCTGGACATCACCCGCCGCCCCGACGGCACGGGGGTGCAGCACGTCGCCTACTCGCGCGGGCCGCACTACTGCCTCGGCGCCATGCTCGCCAACCAGGAGGTGGAGCTGGCGCTGGAGACGCTCTTCGGCCGCTTCCCCGGGCTGGCGCTCGCCGTGCCCGAGAAGGAGCTGACGTGGCGCCCCGTCCCCGGGACCCGGCAGCTCGACCGGCTCCCCGCGGTGTTGGGCGCCGGCGCCTCCGAGGCCGCCTCTCGGTGACCCGGTATCCCGGGGCGCACACCGAGGCGACGCCACGAGGGGCCTCGCCCGTACGGGCGCGGCCCCTCGTGGCACCGCCTCGGTTCAGCGCCGGCCGGGCAGCTCCTCGGGGTGCCGGCTCCGCAGCGTGTCGAACGCCGCGCTCCAGTACCGGGCGCGCTGGAAGGCGTACGTGGGCAGCTCGACGCGCCGGGCGTCGGTCCCGGCGAACACCGCGTCCCAGTCCACCGCGGTGCCCTGCGCGTACACCCGGGCGAGGGCGGTGGCCACCGCGGTCGTCTCGGCCACGCCGGGCCGCAGCGCGGCCACCGCGTGCTGCCCCGCGCCGTCGAGGAGTTCGGCCACCTCGTGGCCGCCCAGTTCGAGGTGGACCGTTCCGTCCTTGGCGACGGCGCGGGCCTGGTCGGCGATGTCCCTCGGCGTCTGCCCCGGGTCGTGCGGTCCGGTCAGCGCCGCGCAGGCGTCGGGCAGTGCCAGCCGTCCGCCGATATGTTCCTCGGTGATGTGGCCGATGCCGTGGCCGTGTACGGCGCCGGGCCGCACGCCCCACGACTCGGCCAGGCGGAACAGCGCCACCTGGACCGCGAAGGCCCTGGCCGCTTCGAGGCCCGCGGCCGGGGCGGGGGCCGCGCCGGTGAGCATCGCGTCACGGGCGGGGAACCCCAGGTGGGCGTCGAGCGCCGCGCAGACCGTGTCGAACGCGCCGGCGAAAGCCGGGAAGGCGTCGTAGAGCTGCTTGCCGGTGCCGGGGGCGAGGGCGTGGCCGGTGAGCAGGACGACGAGCCGGTGGCGGCGGCCGGGCACCCCGTGGACCACGTGGGGCGCGGGTGTCGCGTCGGCCAGCGCCCGCAGGCCGTCCAGCAGGTCCTGGCGGCTCTCGCCGACCACCACGCCACGGTGTCCGAGCGCGGCCCGGGACGTCGCCAGCGACAGGCCGACGTCCGCCGGCCGGAGACCGGGCCGCCGCTCGACATGGGCGAGCAGCCGGGCCGCCTGCGCGCGCAGCGCGTCCGGGTCGCAGCCGGAGACCGGCCAGAGCACCACGCCGCCGCCCGCCGCCTCGGGGGCCGGGGGCGGGGGTTCGTCGTCGGGGGCCTGCTCCAGGATCACATGGGCGTCCGTACCGCCGAAGCCGAACGCGGACACCCCGGCCCGCCGTGGCCGGTCCGTCCCGGGCCACGGTGTGGCGTCGGTGACCAGCCGTATCCGGCCCTCGTCCCAGGGGATGTGCGGGGACGGCGCCTCGGTGTGCAGGGTGCGCGGCACGATGCCGTGGCGCATGGCCTGCACCATCTTGAGCACTCCGGCGATACCGCTGGCCCCCTGCGTATGGCCGAGGTTGGACTTCACCGCGCCCAGCAGCAGCGGCCGGTCGGCGGGGCGACCCTCGCCGTAGACGGCTGCCAGGGCCTGCGCCTCGACCGCGTCGCTGAGCAGTCCGCCGGTGCCCTGGCCCTCGACCGCGTCGACCTCGTGCGGCGCCAGCCCGGAGGCGTCCAGCGCCCTTCTGATCAGCCGCCGTTGGGCGGAACCGTTCGACGCGCTCGCGCCGTTGGTGGCGCCTTCCTGGCCGATCGCGCTGCCGCGGACGACGGCGAACACGGGGTGTCCGTGGGCGCGGGCGGCGGACAGGCGCTCCAGGAGCAGCACCCCGGCCCCCTCGGCGAAGCCGATCCCGTCGGCCCCGTCGGCGAACGAGCGGCAGCGGGCGGCGACAGGCAGCCCTATCCCCCGGGCCGACCCGATGAACGTGTCCGGGCCGCACATCACGGTGACACCGCCGGCCAGCGCCATGGAACACTCGCCGCCGCGCAGCGACTGGCAGGCCAGGTGGAGCGCCACCAGGGAGCCGGAACACGCGGTGTCGAGGGTGGCCGTGGGCCCTTCGAGGCCAAAAGTCAGGGCGATACGGCCGGAGAGCACGGCGCTGGAGCCGCCGGAGGCGACGTGGCCGAGCAGTTCCTCGGG GACGTTCCTCAGGTGCGGGGTGTAGTCCTGGCCGGTGGTGCCCATGAACACGCCGTAGCGCCGGCCGGCGACCGACCGCGGGTCGATCCCGGCCCGTTCGAACGCCTCCCACGAGGTCTCCAGCAGCAGCCGCTGCTGGGGATCCATCACCGACGCCTCGCCCGGGGAGATCCCGAAGGGCGCGGGGTCGAACGCGGCGGCGCCGCCGAGGAATCCGCCCTCGGACACCGCGCTGCGCGCCTGCGGGTGGGCGTCGGGCCCGCACATGCCGTCCAGGTCCCAGCCGCGGTCCCGGGGGAAGGGGCAGATGGCGTCGCGCTCCTCGGCCAGCAGGGTCCACAGGTCGTCCGGGGATTCGACACCGCCGGGGTAGCGGCAGGCCATGCCGACGATCGCGATCGGCTCCCGGGGGGCCGCGCGCAGCCGCTGGTTCTCCTTGCGCAGCCGCTCGGCCTCCTGCAGCGAGGCGCGCAGGGCTTCGACGATCTTGTCGTCGGCCACGGTCATGACATTCCTCCGACGGGACGGCACAGGGGGCGGCGGACAGGACGGCACACGTCAGGACTCGCCGTTGCGCAGGGCCATGTCCACCAGTTCGTCGAGGTCCATGGAGGCGATGTCGGCGGTGTCGGCGTCGCTTTCGTACGGGGCCGGGGCCGGCTCGGACACGGTGGCGTCCGCCTCCGCGTCCGTCTCCGTGTCCCCGCCCTCGCCCAGCCGCAGCAGCGCCTCCAGCAGTCCCGACTGCTCAAGACGCCGCAGCGGGATGGCGGCCAGCACCTGGCGGACCCGGCTCTCGAAGGGGTCGGTGGGCGGGGCGATCTGCTCGCGCAGGTGCTGGGCGAGCGCCAGCGGGTTGGCGAAGTCGAAGACGAGCGTGGCGGGCAGCCGCAGGCCGGTCGCGGCCGAGAGCCGGTTGCGCAGTTCGACGGCGGTGAGCGAGTCGAAGCCCAGCTCCTTGAACGTACGGTCGTGGGCGATCTCGTCGGCCGAGCCGTGGCCGAGTACGGCCGCCATGTGCACGCACACCGCCTCCAGTACACAGCCCTCGCGGTCCGCCTCCGGCAGCGCGGCGAGCCGTTCGGCCAGCGACGGGCCCGAGGTGTTCGCGGTGCCGCTGTCCGCCACCCGCCGCGCCCTGCTACGGCCGGCGGCCGCACCGCCGGCGGGGCCCTGCGCGGACTGCCGGATCGGGACCAGCAGCGCGTCGCCCGCCGCCTGTGAGCGGTCGAACAGCGCGAGGGCCCGCTCGGTGGGCAGCGGCTCCATACCGGCCTGCCGTATCCGCTGCAGGGCGCCGTCGCCCAGCTCACCGGTGAGGGTGCTGGTCTCCTCCCACATGCCCCAGGCCAGGGACTGGGCGGGCAGCCCGGCTGCCTGGCGGTGCTGGGCCAGAGCGTCCAGGAAGGTGCTGGCGGCCGCGTAGTTGGCCTGCGAGGGGTTGCCGTACACCCCGACGACGGAGGAGAAGAGGACGAACATCGCCAGGTCGGCGTCCTTGGTGAGCTCGTGCAGATGCAGTGCGGCATCCGCCTTCGGCCGCAGGACCCGGGCCAGTTGCCCGGGGGTCAGGGCGTCGACCATGCCGTCGTCGAGGACGCCGGCGCAGTGCACCACCCCGGTCAGGGGGTGTGCGTCGGGGATGCCGGCCAGTGCCTCGGCGAGCTGCGCGCGGTCGCCGACGTCGCAGGCCTCGACGGTCACGGTGGCGCCCAGCTCGGCGAGTTCGTCGCACAGGTCGGTGGCGCCGGGGGCCTGCGGGCCGCGGCGGCTGGTCAGCAGCAGCCGCCGTACGCCGTGGCGGGTGACCAGGTGGCGGGCGACCAGGCCGCCCAGGGTGCCGGTGCCGCCGGTGACCAGGACCGTGCCGTCCGGGTCGAGGGTCCGCGGCAGGGTCAGTACGACCTTCCCGACGTGGGCGGCGCGGCTGATGAGGCGGAAGGCGTCGCGCGCCCGCCGGATGTCATAGGCGCGCACCGGTGGATGGGCCAGCTCGTCGCGTTCGAACTGGCCGAGGATGTCGCTCAGCATGCGGCCGATGCCGTCCTCCCCCGGTTGCCCCAGGTCGAGGACGCGGTAGTGGACACCGGGGTGGACCGCGGCGAGCTGCCCGGGGTCGCGGACGTCGGTCAGGCCCAGCTCCACGAACCGGCCGTCCTTGGCCAGCAGGCGCAGCGAGGCGTCGGTGGCGGCGCCGGTGAGGCTGTTGAGGACCACGTCGACGCCGCGGCCGCCGGTGGCGGCGCGGAAGGCTTCCTCGAAGTCGAGGTCGCGGGAGGAGGCGCGGTGGGCCTCGTCGATGCCCATGGCCTCCAGTACGGCGTGTTTGCCGGGGCTGGCCGTGGCGTACACCTCGGCGCCCAGGTGGCGGGCGATCTGCACGGCGGCCATGCCCACCCCGCCGGCCGCCGCGTGGATGAGCACCGACTCGCCCGCCGTGAGGCCCGCGAGGTCCACCAGCCCGTACCAGGCGGTGACGAACGCGATGGGCACGGCCGCCGCCTGCTCGTCGGTCCAGCCCGGCGGGATCGGCACGACGGAGCGGGCGTCGGCCACGGCGGTCGCCCCGAAAGCGCCCTGGAACATGCCCATCACGCGGTCACCGACGGACACCGACGTCACGTCGTCGGCCACCTCGACCACCACGCCCGCGCCCTCGCTGCCCCGGAGGCCCGCCTCGCCCGGGTACATGCCGAGTGCGATCAGTACGTCCCGGAAGTTGACGCCCGCGGCACGGACCTGGACGCGGAGGTGGCCGGGGGCGAGCGGTTGGGGTTCGTCCGCCGCCACCAGGTCCAGGTTGTCCAGGGTGGCGGGGCCGGTGATGTCGAGCCGCCAGTGGGGGGTTTCCGGGAGGGTCAGGGCGCCGCCGGACGCGGCGGGGACCAGGCGGGACACCCGGACCGTCCCGTCCCGGACGACGATCTGCTTCTCCCCCGCGGCCACCGCGGCGGCGACGGCCTCCGGCAGCTCGTGTCCGGCCCCGTCCGCATCCACGTCCAGCAGGACCAGCCGGTCGGGGTGTTCGGACTGCGCGGAGCGGATCAGGCCCCACACCGGGGCGTGCACGAGGTCGGTGACGTCCTCGCCGTCGGTGGCGGCGACCGCGCCACGGGTGACCACTGCCAGCCGCACCCCGGCGAACCGCTCGTCGGCCAGCCACCGCTGCACGGTGTGCAGCACCTCGCCCAGCCGGCGCCGCACGGCCTCGGCCAGGTCCGGGTCGTGCGGCGCCGGATCGCAGGGCAGCACGATCACCGGCGGGAGGGGCAGACCGCCGTCCACCGCGGTGGCGAGCGCGTCGAAGTCGCCGTGGCGGCGCGCCCCGTAGCGGCTGTGCGAGGTGCCGGCGGCGACCGCGGCCCAGTCGTCGGTGGAGACGCGCGGGTCGGGCCCGGCGTCGGTCACAGGGACGTAGGCCACGCGGAAGAGTGCGTCGCGGGTGTCCTCCGCGGCGCCGCTGGGGAGTTGCCCGGCCGTGAACGGCCGGGTGACCAGGGACTCGATCGAGATCACCGGATGTCCC AAGGCGTCGGTGGCGTGGACGGACATGCCGTCGTCGCCGACCGGTGCGAGCCTCACGCGCAGCAACTCGGCGCCGGAGGCGGCCAGCGACACGCCGTTCCAGGCGAACGGCAGCGCCATCCGGCCCGCGGTGTCGCCGCGCACCGCGGTCAGCCCGCTGGCGTGCAGCGCGGCGTCCAGAAGAGCGGGGTGGATGCCGAACGCGGCGGCCTGCCCGCGCTGGTCCTCCGCCAGGACCACCTCGGCGAAGACCTCGCTCTCGCGCCGGTAGACGGCGCGCAGGCCGCGGAAGGCGGGCCCGTAGCGGTAGCCGCCCTCGGCGAGCTGGTCGTAGCAGCCGTCCACGGACACGGGCTCGGCGCCCTCCGGCGGCCACTGGGCGAGGTCGAACGGCTCGGCGGCCCCGCCGGTCGCCAGCGTGCCGCTGACGTGGCGCACCCACGGCTGGTCGGTCCCGGCCGGGCGGGCGTACGCGCTCAAGGGACGGCGGCCGTTCTCGTCGGCCCCGCCCACCACGAGCTGGAGGTCGGTGCCGCCCTCGTCGGCGAGCACCAGTGGGCTGGCCAGCACCATTTCCTCGACACGGTCGCAGCCCACCTCGTCGCCGGCCCGCACGGCCAGCTCCACGAACACGGCTCCGGGCACCAGCACCGCGCCGGCCACCGCGTGGTCGGCCAGCCACGGATGGGTGTCCAGGCCGAGCCGGCCGGTCAGCACCACCTCGTCGCCCTCGGCGAGGACGGTGACGGCGCCCAGCAGCGGATGGTCGGCGGGGGCGAGACCGAGGCGGGTGGGGTCGCCGGTCCCGGTGCCGCCGGCGTCCAGCCAGTAACGCCGGCGCTGGAACGCGTACGTGGGCAGGTCGAGGGGGATGGTCGGGTCCGGGAACACCCGGCTCCAGTCCACCGCGACACCGTGGACATGGGCCTGGGCCAGCGCGAGGAGGAAGCGGTCCCAGCCGCCCTCGTCGCGGCGCAGGGTGCCGAGCGCCGTGGCGGCCACACCTGCTTCGTCGATGGTCTCCTGGGTGCCGATGGCCAGGACCGGGTGCGGGCTGCACTCGATGAACGCACTGTGCCCGTCCTCGAGCAGCGCCTCGGTGGCCTGCTGAAACCGCACCTGCTGACGGAGGTTGGTGAACCAGTACGCGGCGTCCATGGCGGTGCCGTCGATGCGCCGCCCGGTGACGGTCGAGTACAGCGGGACGGAGCCCGCCTTCGGCTCGATGTCCGCGATGGCCTCGGCCAGCCGGTCGCGCAGCGCGTCGACGTGGGCGCAGTGCGAGGCATAGTCCACCGGGACGCGGCGGGCGCGCACCCCGTCGGCCTCGTAGCCCTCCAGGAGTTCGGTCAGGGCGTCGGCGTCGCCGGAGACCACGGTCGAGGAGGGGCCGTTGACGGCGGCCACGCTCAGCCGGCCGTCCCAGCGCTCCAGGTGTTTCCCGACCTGCTGGACGGGCTGGGGCACGGAGGCCATGCCGCCACGGCCGGACAGCTCGGGCAGCGCCTGGGAGCGCAGCACCACGACGCGGGCCGCGTCCTCCACCGACAGCACGCCCGCCACGCAGGCCGCCGCGATCTCGCCCTGGGAGTGGCCGACCACGGCTGCGGGCTCCACACCGTACGAACGCCACAGCTCGGCCAGGGCCACCATGACCACCCACAGCACGGGCTGGACCACGTCGACCCGCTCCAGGGAGGGGGCGTCCGGCTCGCCCCGCAGCACCGACTCCACGGTGTAGTCGGTGTGCCGCTCCACCGCGGCCGCGCAGCGGCGGAAGTGTTCGGCGAACACCGGGGAGGCGTCGGCCAGGGCGAGGGCCATGCCGAGCCACTGCGAGCCCTGGCCGGGGAAGACGAACACCGTCGCGCCGGGGCTCGGCACACCGGTGACCACCTGGCCGGAGGGGGTGCCCTCGGCGAGCGAGGCCAGCCCGGCCAGGAGGGCGTCGCGGTCGGTGGCGACCACGGCGGCGCGGTGCGACTGGGCGGCGCGCGCATGGGCGAGGGTGAAACCGACACGGGTCGGGGTCGCCTCCGGCCGCTCGGCGAGGAAGTCCCGCAGCCGGGCGGCCTGGGCGCGCAGCGCCGCCTCGCCACGCCCGGACACCAGCCAGGGGACGACCGGCAACTCTGCGGCTGGGGCGGGCTCATCGGCGGGCGGGGCCTGTTCGAGGATGACGTGCGTGTTGGTGCCGCTCATGCCGAACGACGACACACCGGCGCGGCGCGGGGCGCCGGTCTCGGGCCACGGCCGCGCCTCGGTCAGCACCTTCACGGTGCCCTCGGACCAGTCCACCTGGGTGGTGGGCTCGTCCACGTGCAGGGTGCGCGGCAGCAGGCCGTGGCGCAGGGCGTACACCATCTTGATCACCCCGGCCATGCCGGCGGCGGCCTGGGTGTGCCCGATGTTGGACTTGACCGAGCCCAGCCACAGCGGGCTGCCCTCGGCGCGCCTCCTGCCGTAGGCGGCGATCAGCGCCTGCGCCTCGATGGGGTCGCCCAGCGAGGTGCCGGTGCCGTGCCCCTCCACCACGTCGATGTCCCGGGCGGACAGCTGGGCGTCGGCCAGCGCGGCGCGGATGACGCGCTCCTGGGCGGGGCCGTGGGGCGCGGTCAGGCCGTTGGAGGCGCCGTCCTGGTTGATCGCGGAGCCGCGGATCACGGCCAGCACCGGATGGCCGAGTCGTTCGGCGTCCGCCAGCCGCTCCACCAGCAGCACACCCACGCCCTCGGCGGGCCCGAAGCCGTCGGCCGCCGCCGAGTACGCCTTGCACCGGCCGTCGCCTGCGAGCCCCTTCAACTTGCTGAACTCCAGGAACGTGCCGGGCGTGGCCATCACGCACACCCCGCCGGCCAGCGCCAGGGAGGACTCGCCCTTGCGCAGCGACTGCACCGCCAGGTGCAGCGCCACCCCGGACGAGGAGCAGGCGGTGTCCACCGTGAAGGAGGGGCCCTCCAGGCCGAGGGTGTACGAGATACGGCCCGACATCGAGCTCGCGGCCAGCCCGGTGGCCATGTACCCGGCGACGTCCTGCGGCGCGTCGGCGCCGCTGCCGGCGTAGTAGATGGTGCTGCCGCCGACGAAGACACCGGTCTTGCTGCCGCGCAGGGAGGTCGCGTCGATACGGGCGCGCTCGATGGCCTCCCAGGCGGTCTCCAGCAGCAGCCGCTGCTGCGGGTCCATCGCCAGGGCCTCGCGCGGCGAGATGTCGAAGAAGTCGGCGTCGAAGTCGGCGATGTCCCGGACGAATCCGCCCTCGCGCACATAGCTGGTGCCCGTCTCGCCCGAGTCGTTGAACAGGCCGGGGTCCCAGCCCCGGTCGTCCGGCATGGACGAGATCACATCGCGCCCGGTGGACACCAGCTCCCACAGGGCCTCCGGGGTGTCCGTCCCGCCGGGGAACCGGCAGGCCATACCGACGATCGCGATCGGCTGGCGGTCCCGGGCCTCCACCTCGCGGAGGCGCTGCTGAGTGTCATGCAGATCGGTCGCCACTCGCTTGAGGTAGTCGACCAGCTGTTC CTGGTTCGCCATGGCGGTCCTCTTTCAGGCCCTTCCCAGCTGGGAGTCAATGAATTGCAGCACTTCATCGGCCGAGGCCGCCTGGAGCCGTTCCGCGACGCCGTTGCCCTCCGCCGCCGCGGGCTGCTGGCGGCACCGCGCCAGCAGCGCTTCGAGCCGGGCCGTCACGGCGTCCCGCTCCTCGTCGCCGGCGAGCAGATCGACGTGGTCCTCCAGCCGGTCCAGTTCCTGGATCAGGGCCTCCGACCCGCTCGGCCCCTCGGAGACGAGCTGGTCCCGCAGGTGCTCGGCGAGCAGCGCCGGGGTGGGGATGTCGAAGATGAGCGTGGAGGGCAGCTTGATGTCGGTGGCCTGGCTGAGCCGGTTGCGCAGCTCGACCGCGGTCAGCGAGTCGAAACCGATCTCCTTGAAGGGCCGCGCCGGGTCGACCGCGAGTACGTCGCCGATCCCGAGCACGGCCGCCACGTTGCGGCGCACCAGCTCCAGCAGCGTACGGCTCTGCTCGACCGCCGAGAGGCCCGCCAGGCGCTCCGCCATCGACGCGGCGTCCCAGCCGGCGGGTTGCCCCGCGGCCGCGCGGCGAACCGGTGCCCCCCGCACCAGGCCGCTGAGGATACGGGGCAGGACGTTGGCGCCCGCCAGGTCCCGTAGCGCCGCCGCGTCGAGGCGGACGGGCACCAGCAGGCTCTCGGGCCGCCGCACGGCCGCGTCGAACAGTGCCAGGCCCTGGTCGGAGGACATCGCCCGGATACCGGAACGCTCCATCCGGGCGATGTCGCGCTCGGCCAGGGCGCCGGTGATGCCGCTGCTCTGCTCCCAGTGGCCCCAGGAGAGCGACTGGCCGGGGAGCCCGGCGGCCCCGCGGTCCTGGGCGAGCGCGTCGAGGAAGGCGTTCGACGCCGCGTAGTTGCCCTGGCCCGCGACACCGGCCACGCCGCCGGTCGAGGAGAACAGCACGAACGCGGCGAGGTCCGCCTCACGGGTCAGCTGGTGCAGATGGACCGCCGCGTCCACCTTGGGCCGCAGCACCTTGTGGAGTTGCCCGGCCGTGAGGGACGGGATGGTGCCGTCGTCGGTCACTCCGGCGGCGTGCACGACGCCGGTCAGCGGGTGCGCGGCGGGGATGCCCGCCAGCAGCTCCGCCAGCGCGTCGCGGTCCGCCGTGTCGCAGGCGGCGATTGTGACCTCGGCGCCCAGAGCGGCCAGTTCGTCGCGGAGCTCGGCCGCGCCGGGCGCGTCGCCACCCCGGCGGCTCGTCAGCAGCAGGTGGCGGACGCCGTGGGCGGTGATCAGGTGGCGGGCGAGCATGCCGCCGATGGTGCCGGTGCCGCCGGTGATCAGGACGGTGCCCTCGGTGTCCAGGCGGCGGCCGGGCAGGTCCGGCTCGGTCAGGTCGGTGTCCTGGCCGATACGGGCCAGGCGCGGCACCACCACTCCGCCGTGGCGCACCGCCACCTGCGGCTCGCCCGCCCCGAGCGCGACGGCGAGGGCACTGGGCAGCAGGGCCGCCGCGCCCTGGTCGGCGGAGGTGTCCTGGCCGGCGGCGTCGAGGTCGACCAGCAGGAGCCGGTCGATGTTCTCGAGCTGCGCGGAGCGCACCAGGCCCCACAGGGCGGAGTGCACCAGGTCGGCGACCCTCTCGTCGCCGCCCGTCGACTGGGCGCCACGGGTCACCAGCACCAGCCGGGAGTCGGCGAACCGCTCGTCCGCGAGCCAGTGTTGGACGACCCCGAGAAGGCGCGCGGTCTCGTCGTGGACCGCGGCGGCGAGCCCGGGGTCGGCTTCGGTCACCGCGCCGGCGAGGGGCGCACAGGGCAGCACCACCAGCTCGGCGGGCGGTGTGCCGTCGTCGACGGAGCGCGCCACCTCCGCGAGGTCCGGCGCGGTACGGACGGAATGTCCGGCCCGTTCCAGCGCGTCGGCGACGCCGAGGCCGTCGTCGCCCACCACCTCCCAGGCGCTTTCGGCGAGCACTCCGGGCTTGGGTTCGGCGGAGATCCACTCGACGCGGTACAGCGAGTCGAGCCCGGCATCGTCGCTGCGGCGCAGCTGCTCCAGGGAGACCGGGCGGGAGACCACCGCCTCGGCGGACGCGACCTGACGGCCCGAGGGGTCCGCGACGCTCAACGACACGCCGTCGGCCGTCGCGTTCGGCACCATACGGACACGCAGCGCGGCGGCCCCGGTGGCGTACAGCGACACACCGCTCCAGGTGAACGGGAGCAGCATCTGCCCGTCGGGCGTACCGCCCTGGCCGATGCCACCGGCTTGCAGCGCGGCGTCCAGCAGGGCGGGGTGCAACCCGAAACGCCCGGCCTCCTCCCGAAGTTCCTCAGGCAGAGCGGCCTCGGCGAACACCTCGTCCCCGCGGCGCCAGGCGGCCTTCAGGCCGCGGAACGCCGGCCCGTAGCCGTAGCCCGCCTCGGCCAGGCCGGTGTAGAGACCGTCCAGGGACAGCGGCTCGGCGTCGGCCGGCGGCCACTGGGTGAAGTCGAAGTCGTCCGGCCGGGCGCCCGTGGTGAGGGTGCCGCTCGCATGCCGGGTCCAGGTCCGGCCGGCGTCGTCGTGGCCGTCGGCGCGGCCGTCGTCGCGTGCGTGGACGGTGAAGGCGCGGCTGCCGTCCTGGCCCGGCGCGGAGACGACGAGCTGGAGTTGCAGCGCTCCCTCCTCAGGGAGGATCAGTGGGGCTTCGAGGACCAACTCCTCGACGCGGTCACAGCCGACCTCGTCCCCCGCGCGCACGGCGAGGTCGACGAACGCCGTGCCGGGCAGGAACACCGCACCGGCCACGGCGTGGTCGGCCAGCCAGGAGTGGCCGCCGGTGGAGATCAGGCCCGTGAGCACGACCTCCCCGCTGTCGGCGACCCGGACGGCGGCGCCGAGCAGCGGGTGGCCGGCCGAGCTGAGGCCGAGGCTCCGGGCGTCGCCGGAGCCGTTGAGGGTGGCGGGATCGATCCAGAAGTGCCGGCGCTGGAAGGGGTAGGTGGGCAGGTCGAGGACGGCCGGGGCTCGGGTGGTGGCACCGGAGGCGGCGTCGGCGGTGGCGTTCACCCCGTACCAAGCCTTCCAGTCCACCGGCAGCCCGGCCGCGAAGGCATGGGCCAGCGCCCGGGCCAGCTGCGCCGGACCACCCTGATCCCGCTGAAGGGTCGGCACGGTGGCCACCGCGACCTCGCCCGCCTGCGCGGTCTCCTGGATGCCCACGCTGAGTACCGGGTGCGGACCGGCCTCGACGAGCACGCGGTATCCGTCGGCCAACACGGCCTCGATCGCGGTGGCGAACCGCACCTGACGGCGCAGATTCTCCACCCAGTAGGTCGCGTCCAGCGTGGTGGTCTCCACCCGGCGGCCGGTCATGGCCGAGTAGAACGCGACCCCCGTGCCGATCGGTGTGATCTCCCCGAGCAGCGTGACCAGATCCTCGGCGATCTCATCCACCTGCGGACCGTGCGAGGCATAGTCCACATCGATCAGCCGCGCCCGCGCGCCGGACGCCTCGACCCCCGCGACCACCTCGGCCACCG CCTCGGGCGGACCCGAGATCACGGTGGAACCCGGACCGTTCACCGCGGCCACCACGACCCCGTCACGCCCACCGATGGCCTTCTCGGCCTCCTCAGCCCCCACCGCAAGCGAGGCCATCGCCCCCCGCCCCGACAACACCCGCAACGCCTGCGAACGCAACGCCACCACCCGGGCACCGTCCTCCAGCGACAGCGCCCCGGCCACCACGGCAGCGGCGATCTCGCCCTGTGAATGCCCCACCACCGCCGCAGGCACCACCCCATGGAAACGCCACAGCTCCGCCAACGACACCATCACCGCCCACAACGCGGGCTGCACCACATCCACCCGCGCCACATCCACCCCGTCCTCGCCCCGCAACACACCCGTCAACGACCAGTCGACATACGGCGCCAACGCCGCCTCACACTCCGCGATCCGCCCCGCGAACACCGGCGAAGAATCCAGCAGCTCCACCCCCATCCCCCGCCATTGACCCCCCTGACCCGGGAAGACCAGCACCGGCCCGGCACCCCCCGCCACCGGCTCGGCAGTCACCACACCACCCGACGGCACACCCTCCGCCAACGCCCCCAACCCAGCCAGCAGCTCCTCCCGGTCCTGGCCCACCACCACCGCGCGCTGCTCGAGCGTCGGTCGGCTGGCCACCAGCGACCACCCCACCTGCGCGGGGTCGGCCTGCGCCTCCTCGGCGTACTCGGCCAGCCGCTCGGCCTGGGCCCGCAGCCCCGCGGCCGAGCGCGCGGACACCACCCACGGCACCACACCGCCCACCGGCATCGGGTCGGGCCCGGCCGGAGCGTCCGGCTCCTGGGGCGCCTCTTCCACGATCACATGCGCGTTGGTGCCGGAGATGCCGAACGCCGAGACACCCGCCCGCCGCGGACGCTCCACCTCCTGCGGCCAGGCGGTGGCCTCCGTGAGCAGTTCAACGGCCCCGCTGCCCCAGTCCACATGCGGCGTCGGCTCATCCACATGCAACGTCCTGGGCAACACACCGTGCCGCATCGCCATCACCATCTTGATGACACCGGCAGCTCCGGCGGCGGCCTGGGTGTGGCCGATGTTGGACTTCACCGAGCCGAGCCACAAGGGCCGTTCCCGCGGCCGCTCCTGACCGTAGGTGGCCAGCACGGCCTGCGCCTCGATGGGGTCGCCGAGCGTGGTGCCCGTGCCGTGCGCCTCGACCACATCGACATCCGCCGACGAGAGCCGGGCGTTGGCCAGCGCCTGCCGGATCACCCGCTGCTGCGACGGACCGTTCGGCGCCGTCAACCCATTCGACGCGCCATCCTGGTTGATGGCCGTTCCGCGGATCACGCCCCACACCTGATGACCGTTGCGCCGCGCGTCCGACAGCCGCTCCAACAACAGCAGCCCTACGCCCTCGCCCCACCCCGTACCGTCCGCCGCCCCCGCGAACGCCTTGCACCGCCCGTCAAGCGCCAGCCCCCGCTGCCGCGAGAACTCCACGAAGGCACCCGGCGTGGCCATCACCGTCACGCCACCCGCCAGCGCCAGCGAGCACTCCCCGCCGCGCAGCCCGCGCACCGCCGAGTGGAGCGCCACCAGGGACGACGAGCAGCCCGTGTCCACCGTCACGGCCGGGCCCTCGAGCCCCAGCACATACGACACCCGCCCGGACACCACACACCCGAGATTGCCCGTGCCCTTGTACCCCTCCATGTCCATGCTGGAGATGCCGATGATCGACAGATAGTCGAAGATGGTCGCGCCGACGTAGACGCCGGTGTCAGTGCCGCGCAGCGAGCCGTGGTCGATACCGGCCCGCTCGAACGTCTCCCAGGCGGTCTCCAGCAGCAGCCGCTGCTGGGGGTCCATCCCCACCGCCTCACGCGGGGAGATCCCGAAGAAGTCGGCGTCGAACTCCCCCGCGTCGTAGATGAATCCCCCCTCGCGCACATAGCTGGTGCCGGGGCGGTCCGGGTCCGGGTCGTACAGCGTGTCGAGGCGCCAGCCGCGGTCGGTCGGGAACGCCGACATCGCGTCCCGGCCCTCGGCCACGAGGTCCCAGAGCTGTTCGGGGCTGCTCACCCCGCCCGGGAAGCGGCAGGCCATGCCGACGATGGCGATCGGCTCGTCAGCCTCCCCAGCCGGTCCCGCGGCGGGTACGACGGCGCCGGTGGCGGCCGCCTGTGTGCCGGAGATCTCCCCGAGGAGGTGGCGGGCGAGGTCGTCCGCGGTGGGGTAGTCGAAGATCAGCGTGGTGGGCAGCGGCATCGCGGCGCTGGAGCTCAGCTTGTTGCGCAGCTCGACGGCGGTCAGCGAGTCGAAGCCCATCTCGAAGAAGGGCTTGGTCGCGGACACCGCGTCCGGGCCCGCGTGGCCCAGCACGCCGGCCGCGAGGGCGCGGATGTGCTGCGAGAGTATCTGGTGGCGCTGTTCCGGGGCAGCCGCGGCCAGTTGCCTGCGCAACGGGCTGTCCTCGGACGGCGTCTCCTTCTCCAGCAGTTCGACGGCCTCGGGGATGTCGGCGAGGAGTTGGCTGGCGCGCTGGCCGGCGAATGTCGCGGCGAACCGCCGCCAGTCGAAGTCGGCCACCGTCAGCGCCGTCTCGCCGCGGCCCAGGGCGTCGCCCAGCACCCGCAGCGCCAGCTCCGGCGCCATGGCGGTCAGACCCCGCTCGCGGAAGAAGGCCGCGACCGTGTCATCGGCGGCCATGCCCGCCTCGCCCCAGGGGCCCCACGCCACCGACAGGCCGGGCAGGCCCTCGGCGCCGCGGCGTTCGGCCAGCGCGTCCAGATACGCGTTGGCCGCCGCGTACGCGCCCTGCTGGCCACTGCCCCAGCTGGCGGCGCCGGAGGAGAACATCACGAACGTCGACAGGTCCATGTCGCGCGTCAGCTCGTCGAGCCACCGCGCGCCCCCCGCCTTCGCGGCCAGCACCTGTGCCACCCGGTCCGGGTCCAGGTCGCCGACCGGCGTGTAGTCGCCGACCCCGGCGGCGTGGAAGACCCCGCGCAGCGGCTGCCCTTCCGGCACCCGGGCCAGCAGCCCGGCCACCTCGTCGCGGTCCGCGACATCGCAGGCCACCACGGACACCGAGGCGCCAAGCCCCTCCAGTTCGGCCGTCAGCTCCTCGGTCCCCGGCGCCGTGGGGCCGCGCCGGCTGGCCACGATCACATGGCGGGCCCCGCGCTCCACCACCCACCGTGCGACGCGGCCACCCACTCCGCCCGTCCCGCCGGTCACCAGGACCGACCCCTCGATCCGCCACTCCTCATCCGGCGCGCCGCCCGACGCCGCACGCAACCGGCGTCCCAGCGCGCCCCCGGCGCGCAACGCCACCTGGTCCTCCGTGCCCGCGGCCAGCACCGCGGCCAGCCGGCTCGGCGTCCCCTCCTCCCCGGCCGCCGGCAGGTCGACCAGGCCGCCCCACCGCTTCGGGTACTCCAGGGCCACCACCCGCCCCAGGCCCCACACCTGCGCCTGCACCGGGCTCACCGAGCCGTCCTGGCCGACGGCCACGCCCCCGCGCGTGACACACCACAGGCGCCCGGCGAACTCCAGCTCGTCCAGC GCCTGCACCAGGGCGAGTGTCCCGGCCAGCCCCGCCGACAGGTGCGGATGCTCCGGGTGTCCGGCCTCGTCCAGGCTCAATACGCTGACCAAGCCGACGACTTCGGGCCAGCCCTCCACCTGCCGCAGACGCGTCGCGAGGAGGCCGCGCTCCTGGTCTTCCGGCGCCACCGCGAAGACCGCGCACCGCGCCCCATGGTCCGCGAGCGCCCCGGCCACCAGCTCGGCCACCGGGTCCCCGACCAGCTTCTCGGGCACCACCAGCAGCCATGTACCGCTCAGCCGGGGAGTCTCGTCCAGGCCGGTCACGGCGGACCAGGCGGTCAGGTAGCGCCAGGCGTCGATCCGGGCCTGGTCGCGCGTACGCCGGCGCCACTGCGCCAACACGGGCAGCGCCGGAGCCAGCACCTGCCGCGCGGTGTCCTCCCCCAGGCTCAGCGACGCCCCGAGTCCGGCGAGGTCCCCCGCCTCGATGGCGCTCCAGAAGCGCTCGTCCTCCTCGGAGCCCTTCTCCTGGGAGCCCGGCCATGTGCCCGGCTTCAGCCAGTAGTGCCGGCGCTGGAAGGGGTAGGTGGGCAGGTCGAGGACGGCCGGGGCCCGGGTGGTGGCGGCGTCGGCGTTCGTGCTCACCCCGTACCAGGCCCTCCAGTCCACCGGCAGCCCGGCGGCGAAGGCGTGCGCCAGGGCCCGGGCCAGCTGCGCCGGACCGCCCTGATCCCGCTGCAGGGTCGGCACGGTGGCCACCGCGACCTCACGCTCCTGAGCGGTCTCCTGGATGCCCACGCTCAACACCGGATGCGGGCCCGCTTCCACCAGCACCCGATAGCCCTCGGCCAGCACCGCCTCGATCGCGGTGGCGAAACGGACCTGACGGCGCAGGTTCTCCACCCAGTACCTGGCGTCCAGCGTCGTGGTCTCCACCCGGCCGCCGGTCACCGTCGAATAGAACCCCACCGTGGTGTCCACCGGTGTGATCTGCCCCAGCAGGGCCACCAGGTCCTCGGCGATCTCGTCCACCTGCGGACCGTGCGAGGCGTAGTCCACATCGATCAGCCGCGCCCGACCACCGGACGCCTCCACCACGGCGACCACCTCGGACACCGCCTGCGGCGGCCCGGAGACCACCGTGGAACCAGGCCCGTTCACCGCCGCCACCACAACACCCTCACGCCCCGCGATGGCCCTCTCCGCCTCCTCAGCCCCCACCGCAAGCGAGGCCATCGCACCCCGCCCCGACAACACCCGCAACGCCTGCGAACGCAACGCCACCACCCGGGCACCATCCTCCAGCGACAACGCCCCCGCCACCACCGCGGCAGCGATCTCGCCCTGTGAATGCCCCACCACCGCCGCAGGCACCACCCCATGGAAACGCCACACCTCCGCCAACGACACCATCACCGCCCACAACGCGGGCTGCACCACATCCACCCGCGCCACATCCACCCCGTCCTCGCCCCGCAACACACCCGTCAACGACCAGTCGACATACGGCGCCAAAGCCGCCTCACACTCCGCGATCCGCCCCGCGAACACCGGCGAAGAATCCAGCAGCTCCACCCCCATCCCCAGCCACTGACCCCCCTGCCCCGGGAAGACCAGCACCGGCCCGGCACCCCCCGCCACCGGCTCCGCCGACACCACCCCACCCGACGGCACACCCTCCGCCAACGCCCCCAACCCGGCCAGCAGCTCCTCCCGGTCGCCGCCGATGACCACGGCCCGGTGTTCGAGCATCGCCCGGCCGGCGGCCAGCGACCACCCCACCTCCTCCGCATCGGCGGTGGTCCGCTCGGCGTACTCGGCCAGCCGTCCGGCCTGGGCCCGCAGCCCCGCGGCCGACCGCCCGGAGACCACCCACGGCACCACACCGCCCGGAGCGGTCGGCTCGGTGGTTTCCCCCTCCGTGGGGGGCTCGGGCGCCTCCTCCAGAATCACATGCGCGTTGGTGCCGGAGATGCCGAACGCCGAGACACCCGCCCGCCGCGGACGCCCCGCCTCCTCCGGCCAGGCGGTGGCCTCCGTCAGCAGCTCGACCGCCCCGCCACCCCAGTCCACATGCGGCGTCGGCGCGTCCACATGCAACGTCTTGGGCAACACCCCATGCCGCATCGCCATCACCATCTTGATGACACCGGCGACACCGGCGGCGGCCTGGGTATGGCCGATATTGGACTTGATCGACCCCAGCCGCAGCGGCCGCTCCTCGGGCCGCGCCTGGCCATAGGTCGCCAACAGCGCCTGTGCCTCGATCGGGTCGCCGAGGGTGGTGCCCGTCCCGTGTGCCTCGACCACATCGACATCCGCCGGCGAGAGCCGGGAGTTGGCCAGCGCCTGCCGGATCACCCGCTGCTGCGACGGACCGTTCGGCGCCGTCAGCCCATTCGACGCACCGTCCTGATTGATCGCCGACCCACGCACCACCGCCCACACCCTGTGACCATTGCGCCGCGCATCCGACAACCGCTCAAGCAGCAGCAACCCCACCCCCTCACCCCACCCCGTACCATCCGCCGCCCCCGCGAACGCCTTGCACCGCCCGTCCAGCGCCAACCCCCGCTGCCGCGAGAACTCCACAAACGCACCCGGAGTGGCCATCACCGTCACCCCACCCGCCAACGCCAACGAGCACTCGCCCCCGCGCAGCGCCTGCACCGCGGAGTGCAGCGCCACCAGGGACGACGAGCAGCCCGTGTCCACCGTGACGGCCGGACCCTCAAGACCCAGCACATACGACACCCGGCCGGAGAGCACACATGCGAGGTTCCCGGTGGCGGCGTAGCCCTCCACCTCGCTGCCGGCCATGGCGGTGAGCACCATGTACTCCTGCGAGGTCACCCCGGCGTAGACGCCCGTGCTGCTGCCCACGAGGCCCTGGGGGTCGATGCCGGCGTGTTCGAAGGTCTCCCAGGCGACCTCCAGCAGCTGCCGCTGCTGGGGGTCCATCGCCAACGCCTCACGCGGTGAAATACCGAAGAATTCCGCGTCGAATTCCCCGGCCCCGCGCAGAAAGGTGCCCTCGCGCACATAACTCGTTCCCGGGCTGTCCGGGTCGGGGTCGAAAAGGTTCTCCAGATCCCAGCCGCGGTCCTGGGGAAATGCGGTCAGCCCCTCGCCTCCGGCCGCCACGAGTTCCCACAAGTCCTCCGGGTTCTCCACCCCGCCCGGAAAACGGCACGCCATACCGACGATCGCGATCGGCTCGGGTTCCGCGGACTCCGCTTCGCGCAGACGCTGCCGGGTGTCCCGCAGTTCTGCCGTCACCCACTTGAGGTGGTCGAGAAGCTTCGCCTCGTCCTTCGCCATCGCTGGCGCCTCCCCTCCTGGCGGTTTTCTTCTTGGCTGTCGGCGTCAGAACGTGGTCAGAGTGTGGTCAGAATGTGCAGCGCAGTTCTTTGAAGCCCTCGATGAAGCTCGATGCCACACGGACGGGTTCGCCGTCCACGCGGATGTCCGGCAGTGAGGTGAAGAGTTCGCGGCACATGGCGACGATCTCGATACGGGCGAGATGGGCGCCCAGACAGAAGTGCGGCCCCACCGCGCCGAACG CCAGATGAGGGTTGGGGTCGCGGGTGATGTCGAACCGGTACGGATCCTCGAAGACCTTCTCGTCGTGGTTCGCGGACCAGTAGAAGAGGAAGATCTCGTCGCCCTTGCGGAACCGGTGGCCGTTCATCTCGCAGTCGCCGGTCGCGGTGCGGCGCATCCAGTTGATGGGCGTGCCCACGCGAAGGATCTCCTCGACGGCGTTCTTCGCGTGGGTCTCGAAGTCCGAGAGCAGGAGTTCCCGCTGGTCGGGGTGGGTGGTGAGCAGTACCAGGGCCTGGGCGATGGCGTTGCGGGTGGTCTCCATGCCGGCGTTGACGAGGAGGATGAAGAACGAGACCAGTTCCTGGTGGGTCAGTTGTTCGCCGTCCTCCTGCACCTGGACCAGCTTGGTGATCACATCGCCGGTGGGGTGGGCGAGCCGGTCCTCGCGGAGCCGTCCGATGTAGTTCCCGAGGTCCCGCAGGGCGCCCAGCACGGCGGCCGCCGCGCCCTCGGGGCTGGCCGTGTGTTCCGGGTCGACCCCTCCCATGATGGTGTTGGTCCGCTCGAAGAGGAATTCGTAGTCCTCGCCGGGAATTCCCATCATGGTGCTGAGGACGGCGATCGGCATCTCCGCCGCCGGGCGGATGAAGTCGCCGGGTCCGCGCTCGATGAGCTCGTCCACGATGCGCCGGGCCACCTGCCGTGACATGGCGTCGAACCTGGGAGCCATACCGCGGCCGAACGACCGCGCCACGATGCGCCGCAATCGGGAGTGCTCCGGATTGTCCATGTTGATCATGGAGCCGTAGAACTCGTCCATCTCCGGCGGCAGGATCGAGGTGGCGCCCTCCGAGGAAAAGTCCTGCGGACGGCGGCTCACTTCCGCGATGTCGGCGTGTTTCACCATCGCGTAATAGCCGTTTGCCAACGGCCCCCAGGGCAGCCGCGGCGGAACGAATTCCGGGTGCGGCAGCCCGCGCAGCCTCTCGAACACCGCCGCCCGCTCCTCATGCGGCCGTAACCAGAAGGACGGATCCATGAAGTCGGGACACGTGGCGGCGGTGGTGGCGGAACGGGACGTGGCTTCCGAAGGAGACACAATGCCTCGATTCAAGCTTGGACAGGTACGAAAACCGGTCCGCGCAAGTCTCACAAGGCCCTCTAAAGAGTTCCTAACAGCCTGAGGGCCCAGGGGCGATTGACGTCGGGGGGACGGGGTGCCAGCCTCGGGGCGTGCAGGTGACGATGACCGGAGGTAGCGGGCGCGATGGATCCACTCGCCGGTCTCCTGGACGGGCCGAGGGCCCGGGGGGCGTTTCTGCTCCGGATGGTCATGGAACCGCCCTGGTCGGTGCGGATCGAGGACCGGGCCCCGCTGTGTGTGATGACCGTGGCGCGCGGTGAGGCGTGGGTGATCCCGGACCGGGGCGGGCCTGAGAAGGGGGTGCGGCTGGGCCCGGGGGATGTCGCGGTCGCGCGCGGCCCCGACCCGTACACGGTGGCCGGCGATCCGGCCACCGAGCCCCAGGCCTGGATCCTGCCGGGCGAGGTGTGCCGGACCGCCGCGGGCGAGGACCTGGCGGAGCGGATGGCGCTGGGCGTGCGGACGTGGGGCAACAGCGAGCACGGCTCGGCGACGATGCTGGTCGGCACGTACCGGATGGACGGCGAGATCAGCCGCCGGCTGCTGGACGCGCTGCCCCCGCTGCTGGTGGTGCCGGGTGAGGCGTGCGACTCGCCGTTGCTGCCGTTGCTCGGCGAGGAGATCGTCAAGGAGGAGGCGGGCCAGACGGCGGTCCTTGACCGGCTGCTGGATCTGGTGCTGATCTCCGTGCTGCGGACGTGGTTCTCCCGCCCGGAGGCGCGGGCCCCGGCCTGGTACCGGGCGCTGGGCGACCCCGTGGTCGGCCGCGCCCTGCGGCTGCTGCAGAACAACCCGGGCCATCCGTGGACGGTGGCCCTGCTGGCCGCGGAGACCGGGAGCTCCCGCGCGGCGCTGGCCCGCCGCTTCACGGAGCTGGTCGGCGAGCCGCCGATGGCGTATCTGACCGGCTGGCGGCTGGATCTGGCCGCCGATCTGCTGCGGGAGCCGGACGCCACCCTCGGGGCGGTGGCCCGCCGGGTCGGCTACGGCAGCTCGTTCGCGCTGAGCGCCGCCTTCAAGCGGGTGCGCGGCGTCAGCCCGCGGGAGCACCGCTCCGCGGCCAGCGCAGGGTGACGGCGGGACCGCGGGCACAGCCGTCCGGCCCGCAGCTGACGGAGCGTTCGTACAGCAGGGTCAGACGGCGGGCGGTGCGGATGATGTCGTAGCGCTCGACGGCGGCGGGCACCGGGTACCGTACGGGGCCGGCGGCGGCCCGCTGGCGCAGGGCGTCGGCCAGGGCGTCGGCACCGGGGCCGACGCGGCGGGCGCCGGGGGCGGCGCCGGGCGGCAGTTCGTCGAGGGCCGGGCAGACGCCGTGGAGCACGGGGAGCCCGGCCGCCAGGGCCTCCACGGCGGCGAGCCCGAACGCCTCCTCGGCGGACGGTGCCACGAACACGTCCATGGCGGCCAGCAGCCCGGGGATGTCCGCGGGCCGGTCCCCGGTCCCGGCCACCGCCCCGTCGCGCTCGCCGAGCAGCCGGATGCGGTCGGCCACCCCGAGCCAGGCGGCCAGGTCGTGCAGCGCGGTGCGCTCGGGCCCGTCCCCGACCAGCAGCAGCCGGGCCTGGGGCACCTCGGCCACGGCCCGCAGCGCCACATCGAAGCGCTTGGCCGCCACCAGCCGGCCGATCCCGCCGACGACGAAGGCGTGTGCGGGGATGCCGAGGCGGGCGCGGGCGGCGGCCCGGGCGGCGGGGGTGAAGCGGAAGTGGCCGGCGTCCAGGCCGTTGGGCATCAGGTGGATGCGGTGGTTGGCGACGCCCCAGCCGCGCAGCCGGTCGGCGACGGCGGCCGAGACGGCGACGGTCGCCGAGCCCAGGCGCTCGGTGGCGAGGTAGCGGGCGCGGGTGCCGACGGTGAGCGGGCGGCCGTCGATGTGGCGCTCGCCCAGGCTGTGCTCGGTGGCGATCACGGCGCCGACGCCCGCCAGCCGGGCGGCGATCCGGCCGTGCACGCAGGCCCGGTAGAGATGGGTGTGCACCAGGTCGTAGCCGCCGCCGCGGATGAACCGCGCCAGCCGGGCCGGTGCCATCGGGTCGCGGACGCCGATCGGCAGATGGGTGACGGGCACGCCGTCCGCCCGGATGCCGTGGCCGGCGGTGCCGGGCGCGGCCAGGGTGAGGACGTCACAGCGGGCGGGCAGGTGGCGCAGCAGCAGCCGTAGTTGCTGTTCGGCGCCACCCGCGCCGAGGCGCGAGATGATGTGCAGCACTCTCATCGGGCGACCGGGCCCCATCTTCCGTATGTCGCACGCCTTATGGCGCGGGTTTACTCGCGCATCCCGCACCCACAGCCCTCACGCGCGGTCGAAACATGCACGCTGAATCGCATCCGACCATGACAGATGGCGGGATGTAAATCACGTAAGACGCGCGGCGATAGGCCGTCGGAGTGGCGGCCCGCCCG GTGCGTCGGCGAAGCGCGGCAGGGCGCGCCGAAAATCCGATTTGCACAGCCATTCCCCAGCGGATTGTTACCTTCTCATCTCGCGCGATGACATGACGAATGTAGGCCGTCCGGGTCATCGCCGCGCGATATTGCGTCCCCGACGACCCGCGGGAGTGCCCAGATGTCCGCACCTACCCCCGTCAGAGACACGGCCGAGAAGGGTGCCGCGCCCAGTGTCGCTCCCCCGCCCGCGCGGCCCGCGCACCACGAGATGGCCTCCCGCCGCGGGGGCCCGGCCGGGCGTTATGAGTACAAGCGGTACAGCCAGCTGGCGGGCCCGCTGACGCAGCCCCCGACCGGCCGCCCGTACCGGGTGCGCTGCCGGAGCCTGCTGGCGCAGGAGCCGCACCGGGTCCGCGCGGCGCTGCTGCTGTGCGCGGCGCCGGTGTGCTCGGCGCTGCTGCTGCTGTGGCTGCTGCAGCCCCAGCACTGGGTGCACCGCGACGGTGTGGCGGGCTGGGAGACGGCCGCGGACCGGACGATGCTGTTCTCGATCGCGCTGATCGAGGCGTTCCGCCTGTTCACCGTGGTCTCCAACGCGCACGCCACACTGGTGGCCCGCGACCCGGTACCGGTGACCGCCGAGCCCGGGACCCGGGTGGCCTTTCTGACCAGCTTCGTCCCCGGCAAGGAGCCGATCGAGATGGTCCGGGCGACGCTGGAGGGGGCCAAGCAGGTCAGACACGCCGGGGGACTGGACGTATGGCTGCTCGACGAGGGAAATGACGAGGAGGCCAAGGAGCTGTGCGCCCGGCTGGGCGTACGGCATTTCTCCCGCAAGGGCGTCGAGAAGTGGAATCAGCCCAAGGGCTCCTTCCGCGCCAGGACCAAGCACGGGAATTACAACGCCTGGCTTGACGCCCATGGCGACGACTATGAATTCATGGCCTGTGTGGACACCGATCATGTGCCGCTGCCCAACTTCCTCGAGCGCATGATGGGTTACTTCCGCGATCCGGACGTCGCCTTCGTGGTCGGCCCGCAGGTGTACGGAAATTACGACACGGCGGTCACCAAGGCGGCCGAGAGCCAGCAGTTCCTCTTCCACGCGCTGATCCAGCGGGCGGGCAACGCGTACGGCGGCCCGATGTTCGTCGGGACCAACAACGCGGTGCGCATCGCCGCGCTGCGGAGCATCGGCGGTCTTTACGACTCGATCACCGAGGACATGGCCACCGGCTTCGAGCTGCACCGCCGCCGCAACCCCGCCACCCGGAAGAAGTGGCGCTCGGTCTACACCCCGGACGTACTGGCCGTCGGGGAGGGCCCGAGCACCTGGACGGACTTCTTCACCCAGCAGCTGCGCTGGAGCCGCGGCACCTACGAGACGGTGCTCAAGCAGTTCTGGAAGGCGCCGTTCACGCTCTCGCCGGGGAAGCTGCTCAACTACTCGCTGATGATCGCCTACTATCCGATGACCGCGCTCAACTGGATGCTGTCGGCGCTGTCCTGTGCGCTGTTCCTGGTGCTCGGGGCGTCCGGGATCCAGATCCCGGCGGAGATGTGGATGATGCTCTACAGCGACGCCGCGGCGCTGCAGATCGGCCTGTACATCTGGAACCGGCGGCACAACGTCTCCCCGCACGAACCGGAGGGCTCCGGCGGGGTGGCGGGGATGCTGCTCTCGGCGCTGTCCGCCCCGATCTACGCCAAGTCGCTGTGCGACGCGCTGCTGCGGCGCAAGAGCCGGTTCGTGGTCACGCCCAAGGGCGACTCCTCCAGCGCCGACCGGCTGTCCACCTTCCGTATCCATCTGGTGTGGGCCGTGGTCTTCGGCGGCTCGCTGGTCGCCTCGCTGTTCCTGGGCCACACCCACGCGGCGATGCGCACCTGGGCGCTGCTGGCGCTGGCGCTGTGCCTGGCGCCGATGGCTATCTGGTCGGGCGGGCTGCTCCTGGCGCGGCGCGGGCGGCGCACCGCCCCGGCGGTGCCGACGGCCCGCGCCGAGGCCCACTGACGGAGCGCGCCCGCCCCGTCCTCATCTGTCGCGCACCATGGCGTTGCGGGCCAGGTCGAGCGCGTACTCCGTCCACCAACGGCCGGCCGGGGGGCCGCCGTTGCAGGTCCCGTCGGACTCGCCGGGGCGCTTGATCCACAGATACGCGTCGATGGCGGGGTCGCCGGTGCGGGTGCTCGGGGGGTCCCCCAGGGCCCGCCCCGGGGGGTTGCACCAGTTCTCCTGGTCGTCGCTGGCGGAGCGTACGGCGCCGGTCTCACCGCTCTTGCCGTCGTCGCCCTCGCCCCTGGCGGGGCTGAGCGGGCCGTTGCCGTTGCGGCTGGTGTCGATGATGAAGTGGACCCTGCCGAGCTGGGTGGACAGGCGACGGCCGTAGGCGCGGGTCACCGAGGTCGGCTGGAAGTTCGAGACGTTCAGGGCGAAGCCGTCCGCCTGGTCGATGCCGGCCTTCATGAGCGGCTCCACCAGCCGCTCGGTGTCCCTGATCCACCCCGGGTTGCCGGCGTCCAGGTAGATCCGGGTGGCGGGCATCGCGGAGAGCATGGCGACCGCGTCGCGCAGCAGGGCCAGCCGTGGCGCCCGCAGCGGCTTGGGAACGCAGCCGTCGACCACCTGGGCCACCGCGTCGGGCTCCAATACGACAATCGCTTTTCGTTCGCCGATTCCGGAGACGATCTCACGGATCCAGTCCAGATATTCGGCGTCGTTCCTCGCCCCGCCCCGGGAGTATCTTCCGCAGTCCCGGTACGGAATGTGATAGGCGACCAGCACCGGCACCCTGTTCTGCCGCGCCGCGGATTCGGTGACGAACCGGACCCGCTCGCGCGGATGCGGGCCCAGCCATTCGGCGATCGGCTCGTCGGCGATCCGCCGCATGAGCTCCGCCTCCGCGTACCGGCCGGCGCGCCGCTGCTCACGGGCCTGCCGGGCGGCCGGGCTGTCCGGGTTCAC(SEQ ID NO：36)
the explanation of the biosynthetic gene cluster is given by taking the biosynthetic gene cluster of the streptomyces as an example, and the biosynthetic gene cluster comprises a linear polyketide synthase gene module I, a glycosyl synthesis related gene module and a modifying gene module. The modules of the biosynthetic gene cluster of Streptomyces are described in detail below, specifically as follows:
wherein, the I type linear polyketide synthase gene module comprises 11 genes of 3A1, 3A2, 3A3, 3A4, 3A5, 3A6, 3A7, 3A8, 3A9, 3A10 and 3A11, and the specific details are as follows:
according to an embodiment of the invention, the 3a1 gene encodes SEQ ID NO: 1, as shown below. According to the embodiment of the invention, 3A1 is located at 5773-14433 bases of the gene cluster nucleotide sequence, is 8661 base pairs in length, encodes polyketide synthase and is 2886 amino acids in length.
Figure BDA0000889460040000331
Figure BDA0000889460040000341
Figure BDA0000889460040000351
According to an embodiment of the invention, the 3a2 gene encodes SEQ ID NO: 2, as shown below. According to the embodiment of the present invention, 3A2 is located at 14448-21131 th base of the gene cluster nucleotide sequence, is 6684 base pairs in length, encodes polyketide synthase, and is 2227 amino acids in length.
Figure BDA0000889460040000352
According to an embodiment of the invention, the 3a3 gene encodes SEQ ID NO: 3, as shown below. According to the embodiment of the invention, 3A3 is located at 21191-33316 base of the gene cluster nucleotide sequence, has the length of 12126 base pairs, encodes polyketide synthase and has the length of 4041 amino acids.
Figure BDA0000889460040000353
Figure BDA0000889460040000361
Figure BDA0000889460040000371
According to an embodiment of the invention, the 3a4 gene encodes SEQ ID NO: 4, as shown below. According to the embodiment of the invention, 3A4 is located at 33337-45120 bases of the gene cluster nucleotide sequence, has a length of 11784 base pairs, encodes polyketide synthase and has a length of 3927 amino acids.
Figure BDA0000889460040000372
Figure BDA0000889460040000381
According to an embodiment of the invention, the 3a5 gene encodes SEQ ID NO: 5, as shown below. According to the embodiment of the present invention, 3A5 is located at 45166-57099 bases of the gene cluster nucleotide sequence, is 11934 base pairs in length, encodes polyketide synthase, and is 3977 amino acids in length.
Figure BDA0000889460040000382
Figure BDA0000889460040000391
Figure BDA0000889460040000401
According to an embodiment of the invention, the 3a6 gene encodes SEQ ID NO: 6, as shown below. According to the embodiment of the invention, 3A6 is located at 57120-62141 bases of the gene cluster nucleotide sequence, has the length of 5022 base pairs, encodes polyketide synthase and has the length of 1673 amino acids.
Figure BDA0000889460040000402
Figure BDA0000889460040000411
According to an embodiment of the invention, the 3a7 gene encodes SEQ ID NO: 7, specifically the following amino acid sequence. According to the embodiment of the invention, 3A7 is located at 71586-76577 bases of the gene cluster nucleotide sequence, has the length of 4992 base pairs, encodes polyketide synthase and has the length of 1663 amino acids.
Figure BDA0000889460040000412
According to an embodiment of the invention, the 3A8 gene encodes SEQ ID NO: 8, as shown below. According to the embodiment of the invention, 3A8 is located at 91418-101764 bases of the gene cluster nucleotide sequence, has a length of 10347 base pairs, encodes polyketide synthase and has a length of 3448 amino acids.
Figure BDA0000889460040000413
Figure BDA0000889460040000421
Figure BDA0000889460040000431
According to an embodiment of the invention, the 3a9 gene encodes SEQ ID NO: 9, as shown below. According to the embodiment of the present invention, 3A9 is located at 82460-84850 base of the gene cluster nucleotide sequence, is 2391 base pairs long, encodes polyketide synthase, and is 796 amino acids long.
Figure BDA0000889460040000432
According to an embodiment of the invention, the 3a10 gene encodes SEQ ID NO: 10, as shown below. According to the embodiment of the present invention, 3A10 is located at 77532-77846 base of the gene cluster nucleotide sequence, and is 315 base pairs long, and encodes ACP protein of polyketide synthase, and is 104 amino acids long.
MEPTNGSPDD GQLADRLASA APEERQQLLC ELVLRRAAEI LDVPSLDEES NFLENGLSSL 60
TAVKLSKFLM DDTGIEVPLV SIVEHPTSTL LGKYLAEAYA ADAA 104(SEQ ID NO：10)
According to an embodiment of the invention, the 3a11 gene encodes SEQ ID NO: 11, as shown below. According to the embodiment of the present invention, 3A11 is located at 84902-91405 bases of the gene cluster nucleotide sequence, has a length of 6504 base pairs, encodes polyketide synthase and has a length of 2167 amino acids.
Figure BDA0000889460040000433
Figure BDA0000889460040000441
The glycosyl synthesis related gene module comprises 9 genes of 3G1,3G2,3G3,3G4, 3G5,3G6,3G7,3G 8, 3M and 3G9, and the specific steps are as follows:
according to an embodiment of the invention, the 3G1 gene encodes SEQ ID NO: 12, as shown below. According to an embodiment of the present invention, 3G1 is located at 69443-70255 bases of the gene cluster nucleotide sequence, is 813 base pairs long, and encodes glucose-1-phosphate: TTP thymidine transferase, 270 amino acids in length.
Figure BDA0000889460040000442
According to an embodiment of the invention, the 3G2 gene encodes SEQ ID NO: 13, as shown below. According to the embodiment of the present invention, 3G2 is located at 68454-69446 bases of the gene cluster nucleotide sequence, is 993 base pairs long, encodes dTDP glucose-4, 6-dehydratase, and is 330 amino acids long.
Figure BDA0000889460040000443
According to an embodiment of the invention, the 3G3 gene encodes SEQ ID NO: 14, as shown below. According to the embodiment of the present invention, 3G3 is located at base 67107-68411 of the gene cluster nucleotide sequence and is 1305 base pairs in length, encodes NDP-D-glucose-3, 4-dehydratase and is 434 amino acids in length.
Figure BDA0000889460040000444
According to an embodiment of the invention, the 3G4 gene encodes SEQ ID NO: 15, as shown below. According to the embodiment of the present invention, 3G4 is located at 66055-67110 th base of the gene cluster nucleotide sequence, has a length of 1056 base pairs, and encodes NDP-D-glucose-4, 6-dehydratase, NDP-D-glucose-4-isomerase, and NDP-D-glucose-4-reductase having a length of 351 amino acids.
Figure BDA0000889460040000451
According to an embodiment of the invention, the 3G5 gene encodes SEQ ID NO: 16, as shown below. According to an embodiment of the present invention, 3G5 is located at 63620-64924 bases of the gene cluster nucleotide sequence, is 1305 base pairs in length, encodes a glycosyltransferase, and is 434 amino acids in length.
Figure BDA0000889460040000452
According to an embodiment of the invention, the 3G6 gene encodes SEQ ID NO: 17, as shown below. According to an embodiment of the present invention, 3G6 is located at 104167-.
Figure BDA0000889460040000453
According to an embodiment of the invention, the 3G7 gene encodes SEQ ID NO: 18, as shown below. According to the embodiment of the present invention, 3G7 is located at 105793-107538 bases of the gene cluster nucleotide sequence and has a length of 1746 base pairs and encodes glycosyltransferase and has a length of 581 amino acids.
Figure BDA0000889460040000454
According to an embodiment of the invention, the 3G8 gene encodes SEQ ID NO: 19, as shown below. According to the embodiment of the invention, 3G8 is located at 107560-108489 bases of the cluster nucleotide sequence, is 930 base pairs long, and encodes endoglucanase enzyme, and is 309 amino acids long.
Figure BDA0000889460040000455
According to an embodiment of the invention, the 3M gene encodes SEQ ID NO: 20, as shown below. According to the embodiment of the invention, 3M is located at 65099-66031 base of gene cluster nucleotide sequence, and is 933 base pairs in length, and encodes methyltransferase, and is 310 amino acids in length.
Figure BDA0000889460040000461
According to an embodiment of the invention, the 3G9 gene encodes SEQ ID NO: 21, as shown below. According to the embodiment of the present invention, 3G9 is located at 70469-71494 bp of the gene cluster nucleotide sequence, is 1026 bp in length, encodes glycosyltransferase, and is 341 amino acids in length.
Figure BDA0000889460040000462
Wherein, the modifying genes comprise 5 genes of 3O,3I, 3E, 3P1,3P2 and 3P3, and the specific genes are as follows:
according to an embodiment of the invention, the 3O gene encodes SEQ ID NO: 22, as shown below. According to the embodiment of the present invention, 3O is located at 77897-79321 bases of the gene cluster nucleotide sequence, has a length of 1425 base pairs, encodes cyclooxygenase, and has a length of 474 amino acids.
Figure BDA0000889460040000463
According to an embodiment of the invention, the 3I gene encodes SEQ ID NO: 23, as shown below. According to the embodiment of the invention, 3I is located at 79410-80351 bases of the gene cluster nucleotide sequence, is 942 base pairs in length, encodes ketosteroid isomerase and is 313 amino acids in length.
Figure BDA0000889460040000464
According to an embodiment of the invention, the 3E gene encodes SEQ ID NO: 24, as shown below. According to the embodiment of the invention, 3E is located at 76579-77424 bases of the gene cluster nucleotide sequence, is 846 base pairs in length, encodes epoxide hydrolase and is 281 amino acids in length.
Figure BDA0000889460040000465
According to an embodiment of the invention, the 3P1 gene encodes SEQ ID NO: 25, as shown below. According to the embodiment of the present invention, 3P1 is located at 62303-63502 bases of the gene cluster nucleotide sequence, is 1200 base pairs long, and encodes cytochrome P450 protein, and is 399 amino acids long.
Figure BDA0000889460040000466
Figure BDA0000889460040000471
According to an embodiment of the invention, the 3P2 gene encodes SEQ ID NO: 26, as follows. According to the embodiment of the invention, 3P2 is located at 81086-82375 bases of the gene cluster nucleotide sequence, is 1290 base pairs in length, encodes a cytochrome P450 protein, and is 429 amino acids in length.
Figure BDA0000889460040000472
According to an embodiment of the invention, the 3P3 gene encodes SEQ ID NO: 27, as shown below. According to the embodiment of the present invention, 3P3 is located at 101832-103028 bases of the gene cluster nucleotide sequence, is 1197 base pairs in length, encodes cytochrome P450 protein, and is 398 amino acids in length.
Figure BDA0000889460040000473
According to an embodiment of the present invention, the biosynthetic gene cluster further comprises: a regulatory gene module comprising: 3R1 gene, 3R2 gene, 3R4 gene, 3T1 gene, 3T2 gene, 3T3 gene, 3T4 gene and 3T5 gene, wherein the sequence information of the genes is as follows:
according to an embodiment of the invention, the 3R1 gene encodes SEQ ID NO: 28, as shown below. According to the embodiment of the invention, 3R1 is located at 3519-4259 bases of a gene cluster nucleotide sequence, is 741 base pairs in length, encodes a regulatory protein and is 246 amino acids in length.
Figure BDA0000889460040000474
According to an embodiment of the invention, the 3R2 gene encodes SEQ ID NO: 29, as shown below. According to the embodiment of the present invention, 3R2 is located at 4321-5082 bases of the gene cluster nucleotide sequence, is 762 base pairs in length, encodes a regulatory protein, and is 253 amino acids in length.
Figure BDA0000889460040000475
According to an embodiment of the invention, the 3R4 gene encodes SEQ ID NO: 30, as shown below. According to the embodiment of the present invention, 3R4 is located at 103257 and 104207 bases of the gene cluster nucleotide sequence, has a length of 951 base pairs, encodes a transcription regulatory factor, and has a length of 316 amino acids.
Figure BDA0000889460040000476
Figure BDA0000889460040000481
According to an embodiment of the invention, the 3T1 gene encodes SEQ ID NO: 31, as shown below. According to an embodiment of the invention, the 3T1 gene is located in the Streptomyces strain SEQ ID NO: 36, the length of the gene sequence fragment is 1365 base pairs, and the gene sequence fragment encodes membrane integrated transfer protein and has the length of 454 amino acids.
Figure BDA0000889460040000482
According to an embodiment of the invention, the 3T2 gene encodes SEQ ID NO: 32, as shown below. According to an embodiment of the invention, the 3T2 gene is located in the Streptomyces strain SEQ ID NO: 36, the length of 687 base pairs, codes ABC transfer factor, and the length is 228 amino acids.
Figure BDA0000889460040000483
According to an embodiment of the invention, the 3T3 gene encodes SEQ ID NO: 33, as shown below. According to the embodiment of the invention, 3T3 is located at 1 st to 609 nd bases of a gene cluster nucleotide sequence, is 609 base pairs in length, encodes a two-component feedback regulator and is 202 amino acids in length.
Figure BDA0000889460040000484
According to an embodiment of the invention, the 3T4 gene encodes SEQ ID NO: 34, as shown below. According to the embodiment of the invention, 3T4 is located at 1714 bases 515-th of the gene cluster nucleotide sequence, is 1200 base pairs long, codes a chemoreceptor and is 399 amino acids long.
Figure BDA0000889460040000485
According to an embodiment of the invention, the 3T5 gene encodes SEQ ID NO: 35, as shown below. According to the embodiment of the present invention, 3T5 is located at base 1707-2903 of the gene cluster nucleotide sequence and is 1197 base pairs in length, encodes a two-component sensor histidine kinase and is 398 amino acids in length.
Figure BDA0000889460040000486
Referring to fig. 1, along the 5 '-3' end of the genes, 3T3 gene, 3T4 gene, 3T5 gene, 3R1 gene, 3R2 gene, 3a1 gene, 3a2 gene, 3A3 gene, 3a4 gene, 3A5 gene, 3A6 gene, 3P1 gene, 3G5 gene, 3M gene, 3G4 gene, 3G1 gene, 3G9 gene, 3a7 gene, 3E gene, 3a10 gene, 3O gene, 3I gene, 3P2 gene, 3a9 gene, 3a11 gene, 3A8 gene, 3P3 gene, 3R4 gene, 3G6 gene, 3G7 gene, 3G8 gene, 3T1 gene, and 3T2 gene were sequentially connected in series according to the present invention.
In the present invention, "tandem" merely indicates the order of connection of genes, and adjacent genes may be directly connected to each other, or other gene fragments may be present or partial sequences of the connection sites may overlap with each other.
According to some embodiments of the invention, the 3T3 gene, 3T5 gene, 3a1 gene, 3a2 gene, 3A3 gene, 3a4 gene, 3a5 gene, 3a6 gene, 3P1 gene, 3M gene, 3G4 gene, 3G3 gene, 3G9 gene, 3P2 gene, 3R4 gene, 3G7 gene, and 3T2 gene are expressed in the same direction.
According to some embodiments of the invention, the expression direction of the 3T4 gene, the 3R1 gene, the 3R2 gene, the 3G5 gene, the 3G2 gene, the 3G1 gene, the 3a7 gene, the 3E gene, the 3a10 gene, the 3O gene, the 3I gene, the 3a9 gene, the 3a11 gene, the 3a8 gene, the 3P3 gene, the 3G6 gene, the 3G8 gene, and the 3T1 gene is the same.
Microorganisms
According to another aspect of the present invention, there is provided a microorganism. According to an embodiment of the invention, the microorganism encodes the biosynthetic gene cluster described above. The inventors have surprisingly found that the J1001-3 compound can be synthesized efficiently, simply and at low cost by performing a fermentation treatment using the microorganism.
According to an embodiment of the invention, the microorganism is streptomyces. According to a particular embodiment of the invention, the microorganism is Streptomyces endomyces subtype Streptomyces.
According to yet another aspect of the present invention, there is provided a process for preparing a compound of J1001-3. Referring to fig. 2, the method is explained according to an embodiment of the present invention, the method including:
s100 fermentation treatment
According to an embodiment of the present invention, the fermentation treatment is performed using the aforementioned microorganism to obtain a fermented microorganism.
According to a specific embodiment of the invention, the fermentation medium conditions are: soluble starch (30g/L), soybean cake powder (10g/L), yeast extract (2.5g/L) and calcium carbonate (3 g/L). Therefore, the fermentation effect is good, and the yield of the J1001-3 compound is high.
According to a particular embodiment of the invention, the fermentation treatment is carried out for a period of 7 to 8 days.
S200 wall breaking treatment
According to the embodiment of the invention, the fermented microorganism is subjected to wall breaking treatment to obtain a solution containing the J1001-3 compound.
S300 purification
According to an embodiment of the present invention, the J1001-3 compound is purified from a solution containing the J1001-3 compound to obtain the J1001-3 compound.
According to some embodiments of the invention, the solution containing the compound J1001-3 is spin dried, extracted with ethyl acetate, concentrated, and then separated by silica gel column and further purified by semi-preparative HPLC to give the desired product.
According to the method for producing the J1001-3 compound of the embodiment of the present invention, the J1001-3 compound can be efficiently synthesized by performing the fermentation treatment using the aforementioned microorganism. Then, the pure J1001-3 compound can be obtained efficiently, simply and at low cost through microbial wall breaking treatment and separation and purification treatment.
According to some embodiments of the invention, the biosynthetic pathway of the J1001-3 compound is predicted from genetic predictions as shown in fig. 3. On one hand, long-chain fatty acid chains are finally formed by catalyzing the extension synthesis of propionyl coenzyme A and methylmalonyl coenzyme A by a polyketide synthesis gene cluster, and an intermediate product of J1001-3 is obtained by modification of 3O,3I and 3E genes, on the other hand, glycosyl is obtained by catalysis of genes such as 3G1,3G2,3G3,3G4 and 3M, and then is transferred onto the fatty acid chains by glycosyltransferases 3G5,3G6,3G7 and 3G9, and finally J1001-3 is formed by the action of P450 enzymes 3P1,3P2 and 3P 3.
The present invention is described below with reference to specific examples, which are intended to be illustrative only and are not to be construed as limiting the invention.
The scheme of the invention will be explained with reference to the examples. It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention. The examples do not specify particular techniques or conditions, and are carried out according to techniques or conditions described in literature in the art (for example, refer to molecular cloning, a laboratory Manual, third edition, scientific Press, written by J. SammBruke et al, Huang Petang et al) or according to product instructions. The reagents or apparatus used are conventional products which are not indicated by the manufacturer and are commercially available, for example from sigma.
Example 1
Streptomyces endomyces subtype Streptomyces is inoculated to an SFM plate and cultured for about three days to four days. When colonies are formed in the SFM culture medium, selecting single colonies to a seed culture medium for culture for about three days to four days, inoculating the single colonies to a fermentation culture medium according to the amount of 1 percent (the components of the fermentation culture medium are 30g/L of soluble starch, 10g/L of soybean cake powder, 2.5g/L of yeast extract, CaCO 3 3g/L, pH 7.2). The culture conditions are as follows: 30 ℃ and 220 rpm, and culturing for 7 to 8 days.
And when the culture is carried out for 7 th or 8 th day, collecting fermentation liquor, separating the supernatant from the mycelium by high-speed centrifugation, adding the same amount of acetone into the mycelium to break the bacteria, carrying out ultrasonic treatment for 20min, carrying out rotary evaporation on the acetone, extracting the supernatant and the mycelium by using the same amount of ethyl acetate, and repeating the steps once. Concentrating to dryness by rotary evaporation, then carrying out crude separation by using a silica gel column to obtain a crude product detected by using HPLC, and considering that the HPLC result shows that the yield of the strain is higher, carrying out TLC plate spotting on a concentrated sample, developing in an iodine cylinder, removing a very obvious point, dissolving by using methanol, carrying out high-speed centrifugation, taking a supernatant, and carrying out mass spectrometry to determine the position of a target point, wherein the result is shown in figure 4, and the compound is a J1001-3 compound.
Example 2
The sequence information of the biosynthetic gene cluster of the J1001-3 compound is researched by taking Streptomyces endous subtype Streptomyces as a research object, and the specific method comprises the following steps:
1. extraction of DNA
(1) Streptomyces endomyces subtype Streptomyces is inoculated into TSBY culture medium and cultured for 2 days, and then thallus is collected at 7000rpm × 5 min.
(2) The collected cells were discarded from the supernatant, and 30ml of SET buffer was added thereto, and the mixture was dispersed and centrifuged at 7000rpm for 5 minutes. The supernatant was discarded again, 10ml of SET buffer was added, the cells were disrupted, 300. mu.l of lysozyme (100mg/ml) was added, and the mixture was subjected to 37 ℃ water bath for 30 to 60 minutes, 100. mu.l of proteinase K (100mg/ml), 10. mu.l of RNase (10mg/ml) and 37 ℃ water bath for 30 minutes.
(3) To the mixture obtained in step (2), 1.2ml of 10% SDS was added, and the mixture was incubated at 55 ℃ for 2 hours while shaking gently every 15 minutes until the liquid became clear.
(4) 4ml of 5M NaCl was added to the liquid obtained in step (3), mixed well and cooled to 37 ℃. 10ml of chloroform was added, gently mixed for 30 minutes, and centrifuged at 12000rpm for 15 minutes.
(5) And (4) adding 10ml of chloroform into the centrifuged supernatant in the step (4), gently mixing for 30 minutes, and centrifuging at 12000rpm for 15 minutes.
(6) And (4) taking the supernatant centrifuged in the step (5), adding 0.8-time volume of isopropanol, and gently swirling until filamentous DNA appears.
(7) And (3) picking out the DNA in an EP tube, adding 70% ethanol for washing, then pouring out the ethanol, naturally drying, dissolving in a certain amount of double distilled water, and extracting to obtain the DNA of the streptomycete.
2. DNA sequence analysis
The genome was sent to a sequencing company to perform genome sequencing to obtain genome information, and the assembled genome was predicted for the gene encoding the protein using GeneMarkS. Then, an anti SMASH software is utilized to predict a region similar to a J1-001 compound synthetic gene cluster, and a J1001-3 synthetic related gene is excavated by combining the comparison result of proteins in the J1-001 compound synthetic gene cluster in the bacterial coding gene data set. The structure of the gene cluster is shown in FIG. 2, and is responsible for the biosynthesis of compound J1001-3, and the functional analysis of each gene is shown in Table 1.
Figure BDA0000889460040000501
Figure BDA0000889460040000511
In the description of the specification, reference to the description of "one embodiment," "some embodiments," "an example," "a specific example," or "some examples" or the like means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
While embodiments of the invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.
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Figure IDA0000919950730000681
Figure IDA0000919950730000691
Figure IDA0000919950730000701
Figure IDA0000919950730000711
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Figure IDA0000919950730000771
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Figure IDA0000919950730000791
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Figure IDA0000919950730000841
Figure IDA0000919950730000851
Figure IDA0000919950730000861
Figure IDA0000919950730000871
Figure IDA0000919950730000881
Figure IDA0000919950730000891
Figure IDA0000919950730000901
Figure IDA0000919950730000911
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Figure IDA0000919950730000931
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Figure IDA0000919950730000951
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Figure IDA0000919950730000981
Figure IDA0000919950730000991
Figure IDA0000919950730001001
Figure IDA0000919950730001011
Figure IDA0000919950730001021
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Figure IDA0000919950730001041
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Figure IDA0000919950730001061
Figure IDA0000919950730001071
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Figure IDA0000919950730001091
Figure IDA0000919950730001101
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Figure IDA0000919950730001131
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Figure IDA0000919950730001151
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Figure IDA0000919950730001171
Figure IDA0000919950730001181
Figure IDA0000919950730001191
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Figure IDA0000919950730001221
Figure IDA0000919950730001231
Figure IDA0000919950730001241
Figure IDA0000919950730001251
Figure IDA0000919950730001261
Figure IDA0000919950730001271
Figure IDA0000919950730001281
Figure IDA0000919950730001291
Figure IDA0000919950730001301
Figure IDA0000919950730001311
Figure IDA0000919950730001321
Figure IDA0000919950730001331
Figure IDA0000919950730001341
Figure IDA0000919950730001351
Figure IDA0000919950730001361
Figure IDA0000919950730001371
Figure IDA0000919950730001381
Figure IDA0000919950730001391
Figure IDA0000919950730001401
Figure IDA0000919950730001411
Figure IDA0000919950730001421
Figure IDA0000919950730001431
Figure IDA0000919950730001441
Figure IDA0000919950730001451
Figure IDA0000919950730001461
Figure IDA0000919950730001471
Figure IDA0000919950730001481
Figure IDA0000919950730001491
Figure IDA0000919950730001501
Figure IDA0000919950730001511
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Figure IDA0000919950730001541
Figure IDA0000919950730001551
Figure IDA0000919950730001561
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Figure IDA0000919950730001601
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Figure IDA0000919950730001631
Figure IDA0000919950730001641
Figure IDA0000919950730001651
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Figure IDA0000919950730001731
Figure IDA0000919950730001741
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Figure IDA0000919950730001781
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Figure IDA0000919950730001811
Figure IDA0000919950730001821
Figure IDA0000919950730001831
Figure IDA0000919950730001841
Figure IDA0000919950730001851
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Figure IDA0000919950730001871
Figure IDA0000919950730001881
Figure IDA0000919950730001891
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Figure IDA0000919950730002171
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Figure IDA0000919950730002191
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Claims (4)

1. A biosynthetic gene cluster of a compound, wherein the compound has the structure,
Figure FDA0003296179250000011
wherein the biosynthetic gene cluster comprises:
the I type linear polyketide synthase gene module comprises: 3A1 gene, 3A2 gene, 3A3 gene, 3A4 gene, 3A5 gene, 3A6 gene, 3A7 gene, 3A8 gene, 3A9 gene, 3A10 gene and 3A11 gene,
wherein, the 3A1 gene codes SEQ ID NO: 1;
the 3A2 gene encodes SEQ ID NO: 2;
the 3A3 gene encodes SEQ ID NO: 3;
the 3A4 gene encodes SEQ ID NO: 4;
the 3A5 gene encodes SEQ ID NO: 5;
the 3A6 gene encodes SEQ ID NO: 6;
the 3A7 gene encodes SEQ ID NO: 7;
the 3A8 gene encodes SEQ ID NO: 8;
the 3A9 gene encodes SEQ ID NO: 9;
the 3A10 gene encodes SEQ ID NO: 10;
the 3A11 gene encodes SEQ ID NO: 11;
a glycosyl synthesis-associated gene module comprising: 3G1 gene, 3G2 gene, 3G3 gene, 3G4 gene, 3G5 gene, 3G6 gene, 3G7 gene, 3G8 gene, 3M gene, and 3G9 gene; and
a modifier gene module comprising: 3O gene, 3I gene, 3E gene, 3P1 gene, 3P2 gene and 3P3 gene,
wherein the 3G1 gene encodes the amino acid sequence of SEQ ID NO: 12;
the 3G2 gene encodes SEQ ID NO: 13;
the 3G3 gene encodes SEQ ID NO: 14;
the 3G4 gene encodes SEQ ID NO: 15;
the 3G5 gene encodes SEQ ID NO: 16;
the 3G6 gene encodes SEQ ID NO: 17;
the 3G7 gene encodes SEQ ID NO: 18;
the 3G8 gene encodes SEQ ID NO: 19;
the 3M gene encodes SEQ ID NO: 20;
the 3G9 gene encodes SEQ ID NO: 21;
the 3O gene encodes SEQ ID NO: 22;
the 3I gene encodes SEQ ID NO: 23;
the 3E gene encodes SEQ ID NO: 24;
the 3P1 gene encodes SEQ ID NO: 25;
the 3P2 gene encodes SEQ ID NO: 26;
the 3P3 gene encodes SEQ ID NO: 27, or a pharmaceutically acceptable salt thereof.
2. The biosynthetic gene cluster of claim 1, further comprising:
a regulatory gene module comprising: 3R1 gene, 3R2 gene, 3R4 gene, 3T1 gene, 3T2 gene, 3T3 gene, 3T4 gene and 3T5 gene,
wherein, the 3R1 gene codes SEQ ID NO: 28;
the 3R2 gene encodes SEQ ID NO: 29;
the 3R4 gene encodes SEQ ID NO: 30;
the 3T1 gene encodes SEQ ID NO: 31;
the 3T2 gene encodes SEQ ID NO: 32;
the 3T3 gene encodes SEQ ID NO: 33;
the 3T4 gene encodes SEQ ID NO: 34;
the 3T5 gene encodes SEQ ID NO: 35.
3. The biosynthetic gene cluster of claim 2, wherein along the 5 '-3' end of a gene the 3T3 gene, the 3T4 gene, the 3T5 gene, the 3R1 gene, the 3R2 gene, the 3A1 gene, the 3A2 gene, the 3A3 gene, the 3A4 gene, the 3A5 gene, the 3A6 gene, the 3P1 gene, the 3G5 gene, the 3M gene, the 3G4 gene, the 3G1 gene, the 3G9 gene, the 3A7 gene, the 3E gene, the 3A10 gene, the 3O gene, the 3I gene, the 3P2 gene, the 3A9 gene, the 3A11 gene, the 3A8 gene, the 3P3 gene, the 3R4 gene, the 3G6 gene, the 3G7 gene, the 3G8 gene, the 3G4 gene, the 3A2 gene, the 3A11 gene, the 3A9 gene, the 3A4 gene, the 3G4 gene, and the like, The 3T1 gene and the 3T2 gene are sequentially connected in series.
4. The biosynthetic gene cluster of any of claims 1 to 3, wherein the nucleotide sequence of the biosynthetic gene cluster is as set forth in SEQ ID NO: shown at 36.
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