CN106916834A - The biological synthesis gene cluster of compound and its application - Google Patents

The biological synthesis gene cluster of compound and its application Download PDF

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CN106916834A
CN106916834A CN201510992001.3A CN201510992001A CN106916834A CN 106916834 A CN106916834 A CN 106916834A CN 201510992001 A CN201510992001 A CN 201510992001A CN 106916834 A CN106916834 A CN 106916834A
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genes
amino acid
acid sequence
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CN106916834B (en
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刘然
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Wuhan Hesheng Technology Co ltd
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Wuhan Zhenzhi Biological Science & Technology Co Ltd
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Abstract

Biological synthesis gene cluster and its application the invention discloses compound.Wherein, the biological synthesis gene cluster of compound includes：I Linear polyketide synthases netic module, the I Linear polyketide synthases netic module includes：2A1 genes, 2A2 genes, 2A3 genes, 2A4 genes, 2A5 genes, 2A6 genes, 2A7 genes, 2A8 genes, 2A9 genes, 2A10 genes and 2A11 genes；Glycosyl synthesis related gene module, the glycosyl synthesis related gene module includes：2G1 genes, 2G2 genes, 2G3 genes, 2G4 genes, 2G5 genes, 2G6 genes, 2G7 genes, 2G8 genes and 2M genes；And modifier module, the modifier module includes：2O genes, 2I genes, 2E genes, 2P1 genes and 2P2 genes.

Description

The biological synthesis gene cluster of compound and its application
Technical field
The present invention relates to bioengineering field, in particular it relates to the biological synthesis gene cluster of J1001-2 compounds and its should With biological synthesis gene cluster, microorganism more particularly, to J1001-2 compounds and preparing the side of J1001-2 compounds Method.
Background technology
Polyketide is one of most monoid of natural products, and doctor is widely used in because of its huge medical value With, the field such as animals and agricultural.Such as antibiotic erythromycin, anti parasitic antibiotic AVM etc..Polyketide is that have Polyketide synthase (PKS) catalysis is formed, and the gene cluster being assembled into modular form determines polyketide structure so as to determine Its bioactivity, therefore a gene cluster for noval chemical compound has particularly important meaning to its follow-up study.
Increasingly serious with pathogenic bacteria drug resistance problems, clinically the demand to antibiotics also seems more urgent. In order to tackle this situation, on the one hand people are transformed existing antibiotic, on the other hand also accelerate new antibiotic sieve The paces of choosing.J1001-2 compounds are a kind of nearest antibiotic for finding, with good bacteriostatic activity.
So far, also without the report of J1001-2 compound biological synthesis gene cluster correlative studys.
The content of the invention
It is contemplated that at least solving one of technical problem present in prior art.Therefore, one object of the present invention It is to propose a kind of biological synthesis gene cluster of the antibiotic-J1001-2 compounds with antibacterial activity.
According to an aspect of the present invention, the invention provides a kind of biological synthesis gene cluster of J1001-2 compounds, its In, the J1001-2 compound structures are as follows,
Embodiments in accordance with the present invention, the biological synthesis gene cluster includes：
I Linear polyketide synthases netic module, the I Linear polyketide synthases netic module includes：2A1 genes, 2A2 genes, 2A3 genes, 2A4 genes, 2A5 genes, 2A6 genes, 2A7 genes, 2A8 genes, 2A9 genes, 2A10 genes and 2A11 genes；
Glycosyl synthesis related gene module, the glycosyl synthesis related gene module includes：2G1 genes, 2G2 genes, 2G3 Gene, 2G4 genes, 2G5 genes, 2G6 genes, 2G7 genes, 2G8 genes and 2M genes；And
Modifier module, the modifier module includes：2O genes, 2I genes, 2E genes, 2P1 genes and 2P2 bases Cause.
Inventor has surprisingly found that the biological synthesis gene cluster of the J1001-2 compounds of the embodiment of the present invention can be controlled The biosynthesis of J1001-2 compounds.
Embodiments in accordance with the present invention, the 2A1 gene codes SEQ ID NO：Amino acid sequence shown in 1；The 2A2 Gene code SEQ ID NO：Amino acid sequence shown in 2；The 2A3 gene codes SEQ ID NO：Amino acid sequence shown in 3 Row；The 2A4 gene codes SEQ ID NO：Amino acid sequence shown in 4；The 2A5 gene codes SEQ ID NO：Shown in 5 Amino acid sequence；The 2A6 gene codes SEQ ID NO：Amino acid sequence shown in 6；The 2A7 gene codes SEQ ID NO：Amino acid sequence shown in 7；The 2A8 gene codes SEQ ID NO：Amino acid sequence shown in 8；The 2A9 genes are compiled Code SEQ ID NO：Amino acid sequence shown in 9；The 2A10 gene codes SEQ ID NO：Amino acid sequence shown in 10；Institute State 2A11 gene code SEQ ID NO：Amino acid sequence shown in 11.
Embodiments in accordance with the present invention, the 2G1 gene codes SEQ ID NO：Amino acid sequence shown in 12；It is described 2G2 gene code SEQ ID NO：Amino acid sequence shown in 13；The 2G3 gene codes SEQ ID NO：Amino shown in 14 Acid sequence；The 2G4 gene codes SEQ ID NO：Amino acid sequence shown in 15；The 2G5 gene codes SEQ ID NO： Amino acid sequence shown in 16；The 2G6 gene codes SEQ ID NO：Amino acid sequence shown in 17；The 2G7 genes are compiled Code SEQ ID NO：Amino acid sequence shown in 18；The 2G8 gene codes SEQ ID NO：Amino acid sequence shown in 19；Institute State 2M gene code SEQ ID NO：Amino acid sequence shown in 20.
Embodiments in accordance with the present invention, the 2O gene codes SEQ ID NO：Amino acid sequence shown in 21；The 2I Gene code SEQ ID NO：Amino acid sequence shown in 22；The 2E gene codes SEQ ID NO：Amino acid sequence shown in 23 Row；The 2P1 gene codes SEQ ID NO：Amino acid sequence shown in 24；The 2P2 gene codes SEQ ID NO：25 institutes The amino acid sequence for showing.
Embodiments in accordance with the present invention, the biological synthesis gene cluster is further included：Regulatory gene module, the regulation base Because module includes：2R1 genes, 2R2 genes, 2R3 genes, 2R4 genes, 2T1 genes, 2T2 genes, 2T3 genes, 2T4 genes and 2T5 genes.
Embodiments in accordance with the present invention, the 2R1 gene codes SEQ ID NO：Amino acid sequence shown in 26；It is described 2R2 gene code SEQ ID NO：Amino acid sequence shown in 27；The 2R3 gene codes SEQ ID NO：Amino shown in 28 Acid sequence；The 2R4 gene codes SEQ ID NO：Amino acid sequence shown in 29；The 2T1 gene codes SEQ ID NO： Amino acid sequence shown in 30；The 2T2 gene codes SEQ ID NO：Amino acid sequence shown in 31；The 2T3 genes are compiled Code SEQ ID NO：Amino acid sequence shown in 32；The 2T4 gene codes SEQ ID NO：Amino acid sequence shown in 33；Institute State 2T5 gene code SEQ ID NO：Amino acid sequence shown in 34.
Embodiments in accordance with the present invention, along 3 ' -5 ' ends of gene, the 2T2 genes, the 2T1 genes, the 2G8 Gene, the 2G7 genes, the 2G6 genes, the 2R4 genes, the 2R3 genes, the 2P1 genes, the 2A8 genes, It is the 2A11 genes, the 2A9 genes, the 2P2 genes, the 2I genes, the 2O genes, the 2A10 genes, described 2E genes, the 2A7 genes, the 2G5 genes, the 2G1 genes, the 2G2 genes, the 2G3 genes, the 2G4 bases Cause, the 2M genes, the 2A6 genes, the 2A5 genes, the 2A4 genes, the 2A3 genes, the 2A2 genes, institute 2A1 genes, the 2R2 genes, the 2R1 genes, the 2T5 genes, the 2T4 genes and the 2T3 genes is stated to go here and there successively Connection.
Embodiments in accordance with the present invention, the biological synthesis gene cluster has SEQ ID NO：Nucleotides sequence shown in 35 Row.
According to another aspect of the present invention, the invention provides a kind of microorganism.Embodiments in accordance with the present invention, it is described micro- Biology has foregoing biological synthesis gene cluster.Inventor, can be with height it has surprisingly been found that carry out fermentation process using the microorganism Imitate, simply and at low cost synthesize J1001-2 compounds.
Embodiments in accordance with the present invention, the microorganism is streptomycete.
According to another aspect of the invention, the invention provides a kind of method for preparing J1001-2 compounds.According to this hair Bright embodiment, the method includes：Fermentation process is carried out using foregoing microorganism, so as to the microorganism after being fermented；Will Microorganism after the fermentation carries out broken wall treatment, to obtain the solution containing the J1001-2 compounds；And from described The J1001-2 compounds are purified in solution containing the J1001-2 compounds, to obtain the J1001-2 compounds Sterling.
The method for preparing J1001-2 compounds according to embodiments of the present invention, is carried out at fermentation using foregoing microorganism Reason, can be efficiently synthesized J1001-2 compounds.Then, by microorganism broken wall treatment and separation and purification treatment, can be with height Imitate, simply and at low cost obtain J1001-2 pure compounds.
Additional aspect of the invention and advantage will be set forth in part in the description, and will partly become from the following description Obtain substantially, or recognized by practice of the invention.
Brief description of the drawings
Of the invention above-mentioned and/or additional aspect and advantage will become from description of the accompanying drawings below to embodiment is combined Substantially and be readily appreciated that, wherein：
Fig. 1 shows the biological synthesis gene cluster sequential structure of J1001-2 compounds according to an embodiment of the invention Schematic diagram；
Fig. 2 shows the schematic flow sheet of the method for preparing J1001-2 compounds according to an embodiment of the invention；
Fig. 3 shows that the flow of the biosynthesis pathway of J1001-2 compounds according to an embodiment of the invention is illustrated Figure；
Fig. 4 shows the collection of illustrative plates schematic diagram of J1001-2 compounds according to an embodiment of the invention.
Specific embodiment
Embodiments of the invention are described below in detail, the example of the embodiment is shown in the drawings, wherein from start to finish Same or similar label represents same or similar element or the element with same or like function.Below with reference to attached It is exemplary to scheme the embodiment of description, is only used for explaining the present invention, and is not considered as limiting the invention.
In the description of the invention, term " longitudinal direction ", " transverse direction ", " on ", it is D score, "front", "rear", "left", "right", " perpendicular Directly ", the orientation or position relationship of the instruction such as " level ", " top ", " bottom " are, based on orientation shown in the drawings or position relationship, to be only For the ease of the description present invention rather than requiring that the present invention must be with specific azimuth configuration and operation therefore it is not intended that right Limitation of the invention.
It should be noted that term " first ", " second " are only used for describing purpose, and it is not intended that indicating or implying phase To importance or the implicit quantity for indicating indicated technical characteristic.Thus, " first " is defined, the feature of " second " can be with Express or implicitly include one or more this feature.Further, in the description of the invention, unless otherwise saying Bright, " multiple " is meant that two or more.
According to an aspect of the present invention, the invention provides a kind of biological synthesis gene cluster of J1001-2 compounds.Root According to embodiments of the invention, the biological synthesis gene cluster includes：
I Linear polyketide synthases netic module, the I Linear polyketide synthases netic module includes：2A1 genes, 2A2 genes, 2A3 genes, 2A4 genes, 2A5 genes, 2A6 genes, 2A7 genes, 2A8 genes, 2A9 genes, 2A10 genes and 2A11 genes；
Glycosyl synthesis related gene module, the glycosyl synthesis related gene module includes：2G1 genes, 2G2 genes, 2G3 bases Cause, 2G4 genes, 2G5 genes, 2G6 genes, 2G7 genes, 2G8 genes and 2M genes；And
Modifier module, the modifier module includes：2O genes, 2I genes, 2E genes, 2P1 genes and 2P2 bases Cause.
Inventor has surprisingly found that the biological synthesis gene cluster of the J1001-2 compounds of the embodiment of the present invention can be controlled The biosynthesis of J1001-2 compounds.
Embodiments in accordance with the present invention, the biological synthesis gene cluster (in the present invention, also referred to as " gene cluster ") is chain The biological synthesis gene cluster of mould, with SEQID NO：Nucleotide sequence shown in 35, it is specific as follows：
ATGGCCGTGAAAGGTCTCAGAAAAGCGATCGCACGATACGGGGCACCGACCGCGGGATGCGTCGTCGCGCTGCTCGC GCTCACCGGATGTCTCGGCTCGCCCGGCGCGCCGGAACGGCGGGGCGGGCTGTGGGTGAACCCGGACAACCCGGCGG CCCGGCACGCCCGTGACCAGCGGCGCGCCGGCCGGTACGCGGACGCGCGGCTCATGCGGCGGATCGCCGACGAGCCG CTGGCCGAATGGCTCGGCCCGAATCCGCGCGACCGGGTCAGGCTCGTCACCGAATCCGCCGCGCGGGAACACCGTAC GCCGGTGCTGGTCGCTTATCACATTCCGTACCGGGACTGCGGGCATTACTCCGCGGGAGGGGCGAGGAACGACAGGG AATACCTTGACTGGATCCGGGAAATCGTCTCCGGAATCGGCGATCGAAAGGCGATTGTCGTATTGGAGCCCGACGCG GTGGCCCAGGTGGTCGACGGCTGTGTCCCCGAGCGGCTGCGGGGCCCGCGGCTGGCCCTGCTGCGCGACGCCGTCGC CATGCTCTCCGCGCCGCCCGGCACCCAGGTGTATCTCGACGCGGGGAACCCGGGGTGGATCCGGAACACCCAGCGGC TGGTGGAGCCGCTCTGGGAGAGCGGCATCGAGCAGGCGGACGGCTTCTCGCTGAACGTCTCCAACTTCCAGCCGACG CAATTGACCCGCGCCTACGGCCACCGGCTGTCGACCCAGCTCGGCGGCGCCCACTTCATCATCGACACCAGCCGCAA CGGCAACGGCCCGCTCACCCGCGGCGGGGAGGGCGGGGCCGAAGACGCCAAGGGCGGCGCGCGGGACGACGCCAAGG GCGGCGGCGGAAGCGCGGGACATCCCGGCACCGGCGCGGGCACCCGCCATCGCGTCGTCGGCGGCGAGGAGAACTGG TGCAACCCCCCGGGCCGGGCGCTGGGCGAGCCCCCGAGCACCCGCACCGGCGACCCCTCCATCGACGCCTATCTGTG GATCAAGCGCCCCGGCGAGTCCGACGGGACCTGCAACGGCGGCCCCCCGGCCGGCCGTTGGTGGACGGAGTACGCCC TCGACCTGGCCCGCAACGCCAAGGCGCGCCACAGGTGACGGCGGGCGGGAGCGGGTGACGGCAGACGGGCGTGGATG AGGGCACGGGCCGTCAGCCCGCCCCGGCGTGGGCCTCGGCGCGCGCCTCCGCGGGCGCCTCGGCGCGGGCCGTCGGC ACCGCCGCGGCGGTGCGCCAGGTGCGCCGCGCCAGCAACAGCCCGCCCACCCAGATGGCCATCGGCGCCAGGCACAG CCCCACCGCCAGCAGCGCCCAGGTGCGCATCGCCGCGTGGGTGTGCCCCAGGAACAGCGAGGCGACCAGCGAGCCGC CGAACACCGCGGCCCACACCAGATGGATACGGAAGGTGGACAGCTGGTCGGCGCTGGAGGAGTCGCCCTTCGGCGTG ACCACGAACCGGCTTCTGCGCCGCAGCAGCGCGTCGCACAGCGACTTGGCGTAGATCGGGGCGGACAGCGCGGACAG CAGCATCCCCGCCACCCCGCCGGAGCCCTCCGGCTCGTGAGGGGAGACGTTGTGCCGCCGGTTCCACACGTACAGCC CGATCTGGAGCGCCGCGGCGTCGCTGTAGAGCATCATCCACATCTCCGCCGGGATGTGGATCCCGGAGGCGCCCAGC ACCAGGAACAGCGCGCAGGACAGCGCCGACAGCATCCAGTTGAGCGCGGTCATCGGGTAGTACGCGATCATCAGCGA GTAGTTGAGCAGCTTCCCCGGCGACAGTGTGAACGGCGCCTTCCAGAACTGCTTGAGCACCGTCTCGTAGGTGCCCC GGCTCCAGCGCAGCTGCTGGGTGAAGAAGTCCGTCCAGGTGCTCGGGCCCTCCCCGACGGCCAGCACGTCCGGGGTG TAGACCGAGCGCCACTTCTTCCTGGTGACCGGGTTGCGGCGGCGGTGCAGCTCGAAGCCGGTGGCCATGTCCTCGGT GATCGAGTCGTACAGCCCGCCGATGCTCCGCAGCGCGGCGATGCGCACCGCGTTGTTGGTCCCGACGAACATGGGGC CGCCATACGCGTTTCCCGCCCGCTGGATCAGCGCGTGGAACAGGAACTGCTGGCTCTCGGCCGCTTTGGTGACCGCC GTGTCGTAATTGCCGTACACCTGCGGGCCGACCACAAAGGCGATATCCGGGTCGCGGAAGTAACCCATCATCCGCTC GAGGAAGTTGGGCAGCGGAACGTGATCGGTGTCCACGCAGGCCATGAATTCATAGTCGTCGCCATGGGCGTCCAGCC AGGCGTTGTAATTCCCGTGCTTGGTCTTCGCACGAAAGGGCCCCTTCGGCTGATTCCACTTCTCGACACCCTTGCGG GAGAAATGCCGGACGCCGAGCCGCGCGCACAGCTCCTTGACCTCGTCGTCGTTCCCCTCGTCCAGCAGCCATACGTC GAGCCACCCGGCGTGCCTGACCCGCATCGCCCCCTCCAGCGTCGCCCGGACCATCTCGATCGGCTCCTTGCCGGGCA CGAAGCTGGTCAGAAACGCCACCCGGGTCCCCGGCTCGGCGGTCACCGGCACCGGGTCCCGGGCCACCAGCGTGGCG TGCGCGTTGGAGACCACGGTGAACAGGCGGAACGCCTCGATCAGCGCGATCGAGAGCAGCATCGTGGTGTCCGCCGC CGTCTCCCAGGCCGCCACATAGTCGCGGTGCACCCAGTGCTGCGGCTGGAGCAGCCACAGCAGCAGCAGCCCCGAAC ACACCGGCGCCGCGCACAGCAGCAGCGCCGCGCGGACGCGGTGCGGCTCCTGCGCCAG CAGGCTGCGGCAGCGCACCCGGTACGGGCGGCCGGGCGGCGGCTGGGTCAGCGGGCCCGCCAGTTGGCTGTACCGCT TGTACTCGTACGCCGCCGCGGGGCCCCGGCGGCGGGAGGTCGTCCCATGGTGCGCGGGCTGTGCGGGGCGCGGCGCG GCGCGGGGCGTGGCACCCGGCTCGGAAGCGTCTCTGACGGGCGTTGGTGCAGACATCTGGGCACTCCCGCGGGTCGC CTGGGACAAAATATCGCGCGGCGATGACCCGGACGGCTTACATTCGTCAGGTCATCGCGCGAGATGAGAAGGTAACA ATCCCATGGGGAATGACTGTGCAAATCGGATTTTCGGCGCGCCTTGGCGCGCCCCGGTGCTCCCCGTCGCTTCCCGG CGCTGCCCGTCGTATTGGGCGGACCGCCACTCCGACGGCCTATAGCCGCGCGTCTTACGTGATTTACATCCCGACGT CTGTCATGGTCGGATGCGATTCGGCGTGCATGTTTCGACCGCGCGATCCGGGCTGCGTTTCGGCCGCGCGTTTCGGC TGCCGGGTTCGGCATGTGTGAGGAAGCCCGCGCCATGGGGCGGGCGCGACAGGAAAGATGGGGCCCGGTCGCCCGAT GAGAGTGCTGCACATCATCTCGCGCCTCGGCGCTGGTGGCGCCGAGCAGCAACTGCGGCTGCTGCTGCGCCACCTGC CCGCCCGCTGTGACGTCCTCACCCTGTCCGCCCCCGGGGCCGCCGGCCACGGCATCCGGGCGGACGGGGTGCCCGTC ACCCACCTGCCCCTGCGCGGCCGCGACCCGATGGCTTCGGCCCGGCTGGCGCGGTTCATCCGCGGCGGCGGCTACGA CCTGGTCCACACCCATCTCCACCGGGCCTGCGTATACGGCCGGATCGCCGCCCGGCTGGCGGGCGTCGGCGCCGTGA TCGCCACCGAACACAGCCTGGGCGACCGCCACATCGACGGCCGCCCGCACACCGTCGGCACCCGTGCCCGCTACCTC GCCGCCGAGCGCCTGGGCTCGGCGACCGTCGCCGTCTCGGCCGCCGTCGCCGACCGGCTCCGCGCCTGGGGCGTCGC CAACCACCGCATCCACCTGGTGCCCAACGGCCTGGACGCCCGCCACTTCCACTACACCCCGGCCGCCCGCGCCGCCG CCCGCGCCCGCCTCGGCATCCCCGCACATGCCTTCGTCGTCGGCGGGGTGGGCCGGCTGATGGCGGCCAAGCGCTTC GACGTGGCGCTGCGGGCCGTCGCCGAGGTGCCCGGGGCCCGGCTGCTGCTGGTCGGCGACGGTCCCGAGCGCACCGC GCTGCACGACCTGGCCGCCTGGCTCGGGGTGGCCGACCGGGTCCGGCTGCTCGGCGAGCGCGACGGGGCGGTGGCCG GGACGGGGGACCGGCCCGCCGACATCCCCGGGCTGCTGGCCGCCATGGACGTCTTCGTCGCGCCATCCGCGGAGGAG GCGTTCGGCATCGCCGCCGTGGAGGCCCTCGCGGCCGGGCTGCCGGTGCTCTACACCGTCTGCCCCGCCCTCGACGA ACTGCCGCCCGGCGCCGCCCCCGGCGCCCACCGCATCGGCCCCGGCGCCGCCGCCCTGGCCGGCGCCCTGCGCGAAT CCGCCGCCGCCGGCCCCGTACGGTTCCCGGTGCCCGCCGCCGTCGAGCGCTACGACATCGTCCGCACCGCCCGCCGG CTGGCGCTGCTGTACGAACGCTCCGTCAGCTGCGGGCCCGACGGCTGCGCCCGCGGCCCCGCCGTCACCCTGCGCTG GCCGCGGAGCGGTGCTCCTGCGGGCTGACCCCGCGCACCCGCTTGAAGGCCGAGCTGAGCGCGTACGGGCTGCCGTA GCCGACCCGCCGGGCCACCGCCCCGAGGGTCGCGTCCGGCTCCCGCAGCAGATCGGCGGCCAGCGTCAGCCGCCAGC CGGTCAGATACGCCATGGGCGGCTCGCCGACCAGCTCGGTGAAGCGCCGGGCCAGCGCCGCCCGGGAGACCCCGGTC CGCTCGGCCAGCAGGGCCACCGTCCACGGATGGGCCGGATGGTTCTGAAGCAGCCGCAGGGCGCGGCCCACCACCGG GTCGCCCAGCGCCCGATACCAGGCCGGGGCCCGCGCCTCCGGCCGGGCGAACCACGCCCGCAGCACGGAGATCAGCA CCAGGTCGAGCAGCCGGTCCAGCACGGCCGTCTGGCCCGCCTCGTCCTTGACGATCTCCTCGCCCAGCCACGACAAC AACGGTGAGCCGCACTCCTCGCCCGGCAGCACCAGCAGCGGCGGCAGCGCGTCCAGCAGCCGCCGGCTGATCTCCCC GTCCATCCGGTACGTACCGACCAGCATCGTCGCCGAGCCGTGCTCGCTGTTGCCCCAGGTGCGCACCCCCAGCGCCA TCCGCTCGGCCAGATCCTCGCCCTCGGCGGTGCGGCACACCTCGCCGGGCAGGATCCAGGCCTGCGGCCTGGTGGCC GGATCATCGGCCACCGTGTACGGGTCGGGGCCGCGCACGATCGCGACGTCCCCCGGAACCAGCCGCACCCCTTTTTC CGGGTCGCCACCCTCCGGGACCACCCATGCCTCGCCGCGCGCCACGGTCATCACACACAGCGGGGCCCGGTCCTCGA TCCGCACGGACCAGGGCGGTTCCATGACCATACGGAGCAGAAACGCCCCCCGGGCTCTCGGCCCGTCCAGCAGGCCG GCCAGTGGATCCATGGCGCCCGCTACCGCCGTTCGAGGGCGGCCGTGGAGCCCCGTACCACCAGATGCGTCGGCAAC ACCCGCTGCGGGGGCTGCTCGCCCTCGGCCGCGGGGGTGGCCAGCATGCGGAACAGCAGATCGACGGCGGCCCGCGC GCCGTGCGCCTTGGGCACCGAGACCGTGGTCAGCGGCGGCGCCGCCATCCCGGCCAGGGCGATGTCGTCGCAGCCCA CCACGCTCATCTCGTCCGGCACCCGCACCCCCCGCACCGCGAACCTGCTCATCAGACCGAACGCGACGATGTCGTCG AACGCCAGCACCGCGCTCGCCCCGCTGGCCAGCACCACATCGGCGGCCCCGGTGACCCCGCCGGGGGTGCCGATGTG ATGCGCCACGCTGCCCACCTGCACGATCTCCACCAGCCCGGACTCGGCCACCGCCGCCACCCCGGCCGCCCGCTCCC GCGCGGCCCACGAGCTGCGCGGCCCTCCGACATAGGCGATCCTGCGGTGGCCCAGCGCGTGCAGATGGGTCAGCGCG TCGGTGATCCCCTCCACCAGGTCGGCCGTGACGCATCCGAACCCCGGCTCCTTTCGGTGCAGCAGCACGATCGGCTT GTCGGTGAGCTCGCCCAGCGACCCCAACTCCTCGCCGCTCAGATGCGGGGAACACAGCACGATCCCGTCCACCTGCG GGGCCAGGGTGCGGATCGCGTCCAGCTCCAGGCCCTCGTCCCGCTCGGCGTCGCTCAAGAACACCGTGTGGCCGAGC CCCCGGGCCCGCGCCTGCACCCCCTTGCAGATGTCCGCGAAGAAGGGGTTCAGCAGATCCGGGAC GATCACGCCCAGGTGGCCCGTGCGCCCGGTGATCAGCCCGCGCGCGGCCTGGTTGGGCTGATAGCCGAGCCGGTCGG CGGCGGCCCGGACCCGCTCGCGCGTCACGGGGCTCACCGTCCCGCCGGGCGTCAGGGCCCGCGACACGGTGGACGGG GACACCCCCGACGCCTTCGCGACGTCCCGAATCGTCACCTGCACGCCCCGAGGTTGACACCCCGTTCCACCCGCGTC AAACATCGTCCCCCCAGGCCACCACCGGCTTCACGGTGTTAGGAACTCTTAAGACGCCCTTGTGAGACTTGCGCGGA CCGGTTTTCGTACCTGTCCACGCTTGAATCGAGGCACCGTGTCTCCTTCGGAAGCCACGTCCACTTCGTCCACCGCA GCCGCGTCTCCCGACTTCCTCGATCCGTCCTTCTGGGCCCGGCCGCTCGATGAGCGTGCGGCGGTGTTCGAGAGGCT GCGTGGGCTGCCGCACCCCGAATTCGTGTCGCCGCGGCTGCCCTGGGGGCCGATGGCATCTGGCTATTACGCGCTGG CGAAACACGCGGACATCGCCGAGGTGAGCCGGCGCCCTCAGGACTTTTCGTCCGAGGGCGCCACCACGATGCTGACG CCGGAGATGGAAGAGTTCTACGGCTCCATGATCACCATGGACAATCCGGAGCACGCCAGGTTACGGCGCATCGTGGC GCGGTCGTTCGGCCGCAACATGGCGCCCAGGTTCGATGCCATTGCCCGTCAGGTGGCCCGGCGGATCGTGGACGAGC TCATCGAGCGCGGGCCCGGCGACTTCGTCCGCCCGGCGGCGGAGATGCCCATCGCCGTGCTCAGCACCATGATGGGC ATTCCCAGTGAGGACTACGAATTCCTCTTCGAGCGGACGAACATCATCATGGGCGGGCTCGACCCCGAGGTCGCGGC CAGCCCCGAGGACGCGGCGGCCGCCGTGCTGGGCGCCCTGCGGGACCTCGGGGACTACATCGGACGGCTCCGCGAGG ACCGGCTCGCCCGCCCCAGCAACGATGTCATCACCAAGCTGGTCCAGGTACAGGACGACGGAGAACAACTCACCCGC CAGGAACTGGTCTCCTTCTTCATCCTCCTCGTCAACGCCGGCATGGAGACCACCCGCAACTCCATCGCCCAGGCCCT GGTGCTGCTCACCGCCCACCCGGACCAGCGGCAACTTCTGCTGTCGGACTTCGAACGCCATGCGAAGGGCGCCGTCG AGGAGATCCTCCGGGTGGGCACCCCCATCAACTGGATGCGCCGGACCGCCACCGGCGACATCGAGATGAACGGCCAT CGGTTCCGCAAGGGCGACCAGGTTTTCCTCCTCTACTGGTCCGCGAACCACGACGGACACGTCTTCGAGGATCCGTA CCGCTTCGACATCACCCGGGACCCCAATCCGCATCTGTCATTCGGCGCGGTGGGGCCGCACTTCTGTCTCGGCGCCC ATCTCGCCCGGATCGAGATCATCGCCATGTACCGAGAACTCCTGGCCTCACTCCCGGACATCCGCTCGGAGGGCGAG CCCATCCGCCTGGATTCCAGCTTCGTCGAGGGCTTCAAAGGTCTGAACTGCACGTTCTGACGCTAAAAGAGAGGAGG CGCCAGCGATGGCGAAGGACGAGGCGAAGCTTCTCGACCACCTCAAGTGGGTGACGGCAGAACTGCGTGACACTCGG CAGCGTCTGCGCGAAGCGGAGTCCGCCGAACCCGAGCCGATCGCGATCGTCGGAATGGCCTGCCGGTTTCCCGGCGG GGTTGAGAACCCGGACGACTTGTGGAATCTGGTGGCGGCCGGAGGCGAAGGGCTGGTCGATTTCCCGGAGGACCGCG GCTGGGATCTGGAGAACCTGTTCGATCCCGACCCGGACCGGCCGGGGACGAGTTATGCGCGCCAGGGCACCTTTCTG CGCGGCGCCGGAGGCTTTGACGCCGAATTCTTCGGGATATCGCCGCGTGAGGCGGTGGTGATGGACCCCCAGCAGCG CGTGTTGCTGGAGGTCGCCTGGGAGACCTTCGAACACGCCGGCATCGACCCGCACGGCCTCGCGGGCAGCAGCACGG GCGTCTACGCCGGGGTGACCTCACAGGACTACATGGTGCTCACCGCCATGGCCGCCAATGACGTGGAGGGATACGCC GCCACCGGAAACCTCGCCTGTGTCCTGTCCGGGCGGGTGTCCTATGAGTTGGGGCTCGAGGGTCCTGCGGTGACGGT GGACACGGGGTGTTCGTCGTCATTGGTGGCGCTGCATTCCGCCGTGCAGGCGCTGCGCGGTGGCGAGTGTTCGCTGG CGTTGGCGGGTGGCGTGACGGTGATGGCGACGCCGGGTGCGTTTGTGGAGTTTTCGCGGCAGCGGGGGTTGGCGGCG GACGGGCGGTGCAAGTCGTTCGCGGCGGCGGCGGATGGTACGGGGTGGGGCGAGGGTGTCGGGCTGTTGCTGCTGGA GCGGCTGTCGGACGCGCGGCGGAACGGCCACAAGGTGTGGGGGGTGGTGCGGGGTTCGGCGATCAACCAGGACGGTG CCAGCAATGGGCTGACGGCGCCGAACGGTCCGTCGCAGCAGCGGGTGATCCGGCAGGCGCTCGCGAACGCGCGGCTG TCGCCCGCCGATGTGGACGCGGTCGAGGCGCATGGGACGGGCACCACGCTTGGCGATCCGATCGAGGCTCAGGCGTT GCTGGCCACCTACGGTCAGGGGCGGGCGGCCGAGCGGCCGTTGCTGCTGGGGTCGGTCAAGTCCAACATCGGTCACA CCCAGGCGGCTGCCGGAGTGGCGGGCATCATCAAGATGGTCATGGCGATGCGGCACGGGCTGTTGCCCAAGACGCTG CACGTGGACGAGCCGACGCCGCATGTGGACTGGAGCGGCGGAGCGGTCGAACTGCTCAGGGAGGCGACGGCCTGGCC GGACGCGGAGCGCCCCCGGCGGGCGGCTGTCTCGTCGTTCGGCATCTCCGGCACCAACGCCCATGTGATTCTGGAAG AGGCCACAGCACCGGCGGCGGACGTGGCGACCACCGAGCCGACGGTCCCCGGTGGCGTGGTGCCGTGGGTGGTCTCG GGGCGGTCGGCGGCGGCGCTGCGGGCCCAGGCGGAGCGGTTGGCGGAGTTCGCCGAGCAGACCTTCGCCGACCCGCC CGAGGTGGGCTGGTCGCTGGCGGCGGGCCGGGCGATGCTCGACCACCGGGCGGTGGTCGTCAGCGACGACCGGGAGG AGTTGCTGGCCGGTCTGAGGGCGCTGGCCGTGGGGGCGCCGTTCGGTGGTGTGGTGACCGCGGAGCCGGTGGCCGGG GGTGCCGGGCCGGTGCTGGTGTTCCCGGGCCAGGGCGGTCAGTGGCGGGGGATGGGGGTGGAGTTGCTGGACTTCTC CCCGGTGTTCGCGGCGCGGATCGCGGAGTGCGAGGCGGCGCTGTCGCCGTTTGTGGACTGGTCGCTGACGGCGGTGC TGCGGGGCGAGGGCGGGGTGGACGCGTCGCGGGTGGATGTGGTGCAGCCGGCGTTGTGG GCGGTGATGGTCTCGCTGGCGGAGGTGTGGCGTTCCCATGGCGTGGCGCCCGCGGCGGTGGTGGGGCATTCGCAGGG CGAGATCGCGGCGGCCGTGGTGGCCGGGGCGCTGTCGCTGGAGGACGGGGCGCGGGTTGTCGCTGTGCGCTCGCAGG CGTTGGGGGTGTTGTCGGGCCGGGGTGCGATGGCCTCGCTCGCGGTGGGTGCCGGGGAGGCCGAGGAGGCGATCGGC GGGCGTGAGGGTGTGGTCGTGGCGGCGGTGAACGGGCCGGGGTCGACCGTGATCTCGGGTCCGCCCGGGGCGGTGGC CGAGGTGGTGGCGGCGGTGGAGTCGGCCGGTGGGCGGGCGCGGCTCATCGACGTCGATTACGCCTCGCACGGCCCGC AGGTCGACGAGATCGCCGAGGATCTCGTGGCGCTGCTGGGGGAGATCACTCCGGTGGAAACGGCCACGGCGTTCTAC TCGACGGTGACCGGTGGCCGGGTGGAGACCACGGCGCTGGACGCGGCGTACTGGGTGGAGAACCTGCGCCGTCAGGT CCGGTTCGGCACCGCGGTGGAGGCGGTCCTGGGCGAGGGGTACCGGGTGCTGGTGGAGGCCGGCCCGCATCCGGTAC TGAGCGTGGGCATCCAGGAGACCGCCCAGGCATGCGATATCGCGGTGGCCACGGTGCCCACCCTCCAGCGTGACCAG GGCGGCCCGGCGCAGCTGGCCCGGGCCCTGGCCCAGGCCTTCGCCGCCGGGCTGCCCGTGGACTGGAAGACCTGGTA CGGCGTGACCGCAGCCGCGCCACCCGTCCTCGACCTGCCCACCTACCGCTTCCAACACCAGCACTACTGGCTGAAGC CGGGTACCTGGGCGGGGGCCGAGGGAAAGGGCTCCGCAGAGGACGAGCGCTTCTGGAGCGCCGTCGAGAGCGGCGAT CTCGCCGGGCTCGAGGCATCGCTGCGCGTGGGGGAGGACGCCGCGCGACGCGTGCTGGCTCCGGCGCTGCCGGTGCT GGCGGAGTGGCGTCGGCGTACCCGGGATCAGGCCCGGATCGACGCATGGCGCTACCGGACCGCCTGGTCCGCCGTCA CCGGCCCGGACGCGGCTCCCCGGCTGAGCGGCACATGGTTGCTGGTCGTCCCCGAGGAGTGGGCCGGGGACGCCGCG GTCGAGACGGTCACCCAGGCGCTCGAAGGCCATGGGGCGGAGTGCGTGCTCCTCCCGCTGGCCCCGGGGGACCAGGC GCGCGGCCCCCTCGCCGAGCGGCTGCGGCAGTTGGAGGGCCGGCCCGAGATCGTCGGTGTGATCAGCGCCTTGAGCC TCGACGAGGCCGTACACCCGGAGCACCCGCATCTGCCGATGGGACTGGCCGGGACGCTCGCCCTGGTGCAGGCGCTG GACGAGGTGGACTTCGGCGGGCGCCTGTGGTGTGTGACGCGCGGCGGTGTGTCCGCCGCCGGGGACGGCGCCGTAAG CCCGGTGCAGGCCCAGGTGTGGGGTCTGGGGCGCGTGGTGGCGCTGGAGTATCCGAAGCGGTGGGGTGGCCTGATCG ATCTTCCGGCGGTGGCGGAGGAGGGCACGGCGGGCCGGCTGGCCGCGGTGGTGGCCGAGGGCGCGGAGGACCAGGTC GCGCTGCGTGCGGAGGGCGTGCTGGGGCGTCGCCTGCGTGCGGCGTCGGCGGGTGCGCCGGGTGCGGAGTGGCGCAT CGACGGCTCCGTTGTGGTGACCGGCGGGACGGGCGGGGTGGGCGGCCGGGTCGCCCGGTGGGTCGTGGAGCGGGGCG CGCGCCATGTGATCCTGGCAGGCCGCCGGGGCTCCGCCGCGCCGGGGACCGAGGCGCTGACGGCGGAGCTGGAGGCG CTGGGCGCCTCGGTGGACGTGGTGGCCTGCGATGTCGCGGACCGCGACGAGGTGGCCGCGCTGCTGGCGCGGGCGCC GGAAGAGCATCCGCTCCGTGGGGTCTTCCACGCCGCGGGGGTCGGCGACTACACCCCGGTCAGCGACCTGGACCCGG ACCGGGTGGCGCAGGTGGCGGCGGCGAAGGCGGGGGGTGCGCGATGGCTCGATGAGCTGACGCGCGACATGGACGTG TCGGCCTTTGTGCTGTTCTCCTCCGGCGCGGCCAGTTGGGGTAGCGGGCAGCAGGGTGCGTACGCGGCGGCCAACGC GTACCTGGACGCGCTGGCCGAACGCCGCCGCGCCGAGGGCCTGCCGGGTCTGTCGGTGGCATGGGGCCCGTGGGGCG AGGTCGGCATGACCGCCGGCGACGCGGTGGCGGCCTTCTTCCGCGAGCGGGGGCTGACCGCCATGGCTCCCGAGTTG GCGCTGCGGGTACTGGGCGATGCCCTGGGCCGCGGCGAGACGGCGTTGACGGTGGCCGACTTCGACTGGCGGCGCTT CGCCGCGGCGTTCGCCGGGCAGCGCGTCAGCAGGCTGCTCGCCGAGATTCCCCAGGCCGTCGAACTGCTGGAGAAGG AGACGCCGTCCGAGGACAGCCCGCTGCGCAAGCAACTGGCCGCGGCCGTCCCGGAGCAGCGGCACCAGATGCTCACG CAGCACATCCGCGCCCTGGCGGCCGGGGTGCTGGGCCACTCGGGCCCGGACGCGATCTCCGCGACCAAGCCGTTCTT CGAGATGGGCTTTGACTCGCTGACCGCTGTCGAGCTGCGCAACCGGCTCAGCGCCAGCGCCGGGATGCCGCTGCCCA CCACACTGATCTTCGACTACCCCACCGCGGACGACCTGGCCCGTTACGTCCTCGCGGAAATCTCCGGCCTCCAGACG GCCGTGACCGGCACGGTCACGCCGGTCGGGGAATCGGACGAGCCGATCGCGATCGTCGGTATGGCCTGCCGGTTCCC GGGCGGGGTGAGCAGCCCCGAACAGCTCTGGGACCTCGTGGCCACGGGCCGGGACGCGATGTCGCCCTTCCCGGCCG ACCGAGGCTGGCGTCTCGACACGCTGTACGACCTGGACCCGGACCGCCCCGGCACCAGCTATGTGCGCGAGGGGGGA TTCCTCCACGACGCGGGGGGTTTCGACGCGGAGTTCTTCGGGATCTCGCCGCGTGAGGCGGTGGGGATGGACCCTCA GCAGCGGCTGTTGCTGGAGACCGCCTGGGAGACGTTCGAGCGCGCCGGGATCGACCACGGCTCGCTGCGCGGCAGCG ACACCGGTGTCTATGTCGGCGCGACCCTCTTCGACTATCTGTCGATCATCGGGATCTCCAGCATGGACATGGAGGGC TACACCGGCACGGGCAACCTCGGCTGTGTGCTGTCGGGGCGGGTGTCCTATGTGCTGGGGCTCGAGGGGCCGGCGGT GACGGTGGACACGGGGTGTTCGTCGTCGTTGGTGGCGCTGCACTCGGCGGTGCAGGGGTTGCGTGGCGGTGAGTGTT CGCTGGCGTTGGCGGGCGGTGTGACGGTGATGGCGACGCCGGGTGCGTTTGTGGAGTTTTCGCGGCAGCGGGGGTTG GCGGCGGACGGGCGGTGCAAGTCGTTCGCGGCGGCGGCGGATGGTACGG GGTGGGGCGAGGGTGTCGGGCTGTTGCTGCTGGAGCGGCTGTCGGACGCGCGGCGGAACGGCCACAAGGTGTGGGGG GTGGTGCGGGGTTCGGCGATCAACCAGGACGGTGCGAGTAACGGCCTGACGGCGCCGAACGGCCCGTCGCAGCAGCG GGTGATCCGGCAGGCGCTCGCGAACGCGGGGTTGTCGACGGCGGACGTGGATGTCGTCGAGGCGCACGGGACGGGCA CCACGCTTGGCGATCCGATCGAGGCTCAGGCGTTGCTGGCCACCTACGGTCAGGGGCGGTCGGCCGAGCGGCCGTTG CTGCTGGGGTCGGTCAAGTCCAACATCGGTCACACCCAGGCGGCTGCCGGAGTGGCGGGCATCATCAAGATGGTCAT GGCGATGCGGCACGGGGTGGTGCCCAAGACGCTGCACGTGGACGAGGCGACACCGCATGTGGACTGGGGCGGCGGGG CGGTCGAACTGCTCAGGGAGGCGACGGCCTGGCCGGAGGTGGAGCGCCCCCGGCAGGCGGGCGTGTCGGCGTTCGGT ATCTCCGGGACGAACGCGCACGTGATCGTGGAAGAGGCTCCCAAGGAGCCGGAGGCTCCGACCGAGTCCGGCGCGGT GCCGGTGGGTGGTGTGGTGCCGTGGGTGGTCTCGGGACGGTCGGCGGCGGGGCTGCGGGCCCAGGCCGCGCGGCTGG CGGAGTTCGCCGAGCGGACCGCCGCCGATGAGGCGGAGGTCGGCTGGTCGCTGGTGGCCGGCCGAGCCACGCTGGAA CACCGCGCGGTGGTCGTCGGCCAGGACCGGAAGGAGCTGCTGGCCGGGTTGACGGCGCTGGCCGAGGGCGAGCCGTC CGGTGGTGTGGTGACCGCCGAGCCGGTGGCCGGGGGTGCCGGGCCGGTGCTGGTGTTCCCGGGCCAGGGCGGTCAGT GGCGGGGGATGGGCGTGCAACTGCTCGACGCCTCGCCGGTGTTCGCGGCGCGGATCGCCGAGTGCGAGGCCGCGCTG TCGCCCTTCGTGGACTGGTCGCTCACGGCGGTGCTGCGGGGCGTCGACGAGACGGCGGACATCGACCGGGTGGATGT GGTGCAGCCGGCGTTGTGGGCGGTGATGGTCTCGCTGGCGGAGGTGTGGCGCTCCCATGGCGTGGCGCCCGCGGCGG TGGTGGGGCATTCGCAGGGCGAGATCGCGGCGGCCGTGGTGGCCGGGGCGCTGTCGCTGGAGGACGGGGCGCGGGTT GTCGCTGTGCGCTCGCAGGCGTTGGGGGTGTTGTCGGGCCGGGGTGCGATGGCCTCGCTCGCGGTGGGTGCCGGGGA GGCCGAGGAGGCGATCGGCGGGCGTGAGGGTGTGGTCGTGGCGGCGGTGAACGGGCCGGGGTCGACCGTGATCTCGG GTCCGCCCGGGGCGGTGGCCGAGGTGGTGGCGGCGGTGGAGTCGGCCGGTGGGCGGGCGCGGCTGGTCGATGTGGAC TACGCCTCGCACGGCCCGCAGGTCGACGAGATCGCCGAGGAGCTGGTGGCGTCGCTGGGAGACGTCAAGCCCGCCGG CACCGGGGTGGCGTTCTATTCGACGGTGACCGGTGGCCGGGTGGAGAGCACGGTGCTGGACGCGGCGTACTGGGTGG AGAACCTGCGCCGTCAGGTCCGGTTCGGCACCGCGGTGGAGGCGGTGCTGGCCGACGGATACCGGGTGCTGGTGGAG GCCGGTCCGCATCCGGTGCTGAGCGTGGGCATCCAGGAGACCGCCCAGACGCTGGACCTTCCCGTGGCCACGGTGCC CACTCTCCAGCGTGACCAGGGTGGCCCGGCGCAGCTGGCCCGGGCCCTGGCCCAGGCCTTCGCCGCCGGGCTGCCCG TGGACTGGAAGACCTGGTACGGCGTGACCGCAGCCGCGCCACCCGTCCTCGACCTGCCCACCTACCCCTTCCGACAC CAGCACTACTGGATCGACCCCGCCACCATCGGCGGCCAGGGCGATCCCCACAGCCTCGGCCTGACCTCCGCCGACCA CCCGCTGCTCGGCGCCGCCGTGCGGGTCGCCGAGAGCGAGGAGATCGTGCTGACGGGCCGGGTCTCCACCGCCGGAC ACCCCTGGGTGGCCGACCACGCCGTGGCCGGTTCGGTGTTCCTGCCCGGCACGGCGTTCGTCGACCTCGCCGTGCGC GCGGGGGACGAGGCAGGCTGTGACCGCGTCGAGGAACTGGTCCTGGAAACCCCCCTGATCCTCCCCGAAGAGGGGGC GATCCAGCTCCAGCTCGTCGTCTCCGCGCCAGGCCAGGACGGCGGTCGAGCCTTCACCGTCCACGCGCGCGCCGAGG GCGACGACGACGCCGACCGGCCCTGGACCCGGCACGCGAGCGGCACCCTCACCACAGGCGCCCGGCCGGACGACTTC GACTTCGCCCAGTGGCCGCCGACCGGTGCCGAGCCGGTGCCGCTGGACGGTCTCTACGCGGGCCTGGCCGAGGCGGG CTACGGCTACGGCCCGGCGTTCCGCGGCCTGAAGGCGGCCTGGCGCCGCGGTGACGAGGTGTTCGCCGAGGCCGCGC TCGCCGAGGAACTGCGCGGCGACGCGGGGCGGTTCGGTCTGCACCCCGCCCTGCTGGACGCAGCGCTCCAGGCCGGC GGCATCGGGAAGGGCGGTGCGCTCGACGGGCAGCTGCTGCTGCCGTTCACCTGGACCGGAGTGTCGCTGTACGCCAC CGGCGCCGCCGCGCTGCGGGTCCGGATGGTTCCAGGCGGCGACGGGGTGGCCCTGAGCGTCGCGGACCCCTCGGGCC GCCCGGTCGCGTCCGCCGAGGCGGTGGTCTCCCGCCCGGTCTCCATCCGGCAGTTGCGCCGCGGCGGTGCCGAGCTC GACTCGCTGTACCGCGTCGAGTGGGTCTCCACCGAGTACGAGCCCGAGCCCGGGGTGTTCGCCGGCCGTACGTGGGA CGTGGTGGGTGCCGGCGGCCTCGGCATCGAGGACGCACTGGAACGCGCAGGACACACCGTCCGCACCACCCGGGACC TCGCGGAGCTGGCGCGGTCCGTCGACGACGGGACACCCCTGGCCGACCTGGTGGTACTGGACTGCGCCCCGCTCGCC GGTGGCGGAACCGAAGCCGACCCCGGACTCGCCGACGCGGTCCACGACGAGACCGAACGGCTGCTCGGCGTCGTCCA ACGCTGGCTCGGGGACGAGCGGTTCGGCGACTCCCGGCTGGCGCTGGTGACCCGCGGCGCCCAGTCGACCAGCGGCG ACGAGCGCGTCGCCGACCTGGTGCACGCACCGCTGTGGGGCCTGGTGCGCTCGGCGCAGATCGAGAGCTACGACCGG CTCGTGCTGGTGGACCTGGAGGACGGCGACCAGGACGCGGCCGGCCTGCTGCCCCGCGCCCTCGCCGTGGCGCTCGG GGCGGGCGAGCCGCAGGTGGCGGTGCGCCGCGGCCGTCTGGTCGTCCCGCGCCTGGCCCGTATCGGCAAGGACACCG CCCTCACCCCGCCGGACGTGCCCGGCTGGCGCCTCGACACAGC CCGTCCCGGCACTCCCGGCACTCCCGGCACTCCCATCGCTCCGGACCAGTTGGCCCTGGTGCCTCACCCCGAGGCGG CCGGGCCGCTGGGGGAGGGCCAGGTCCGCATCGCCGTACGGGCCGCGGGCCTCAACCCCCACGACGTCGTCGCGGGC CCGGACCCGCTTCCCGACCAGCAGCCCATGGGCGGTGAGGGGGCCGGGGTCGTCGTCGAGACGGGGCCCGGTGTCAT CGGACTCGCCCCCGGCGACCGTGTGATGGGCGTCTTCCCTCGCGCGTTCGGCCCGCTGGCCGTCGCCGACCAGGGGA GCCTCGTACCAGTCCCCGCCGGCTGGTCCTACGCCCGGGCCGCCGCCGTGCCCCTGGCGTTCCTCACCGCCTACTGC TGCCTCGCCGAGCCCGCCGAGGTCCGTGAGGGCGACGTGGTGCTCGTCCACGGGGCCGCCGGTGGCGTCGGCATGGC CGCCGTGCAACTGGCCCGGCATCTCGGCGCCGAGGTCTATGCCACCGCCGATGAGAGCGAGTGGGACGCCCTGCGCG CCCTCGGCCTGGACGACGACCACCTGGCCTCCTCCCGGACCCTCGACTTCGAGGACCGGGTGCGCGCCGCCACCGGC GGCCGGGGCGTGGACGTGGTGCTGAACTCCCTCGCCCAGGAGTTCACGGACGCCTCGCTGCGCCTGCTGCGCGACGG CGGCCGGTTCGTGGAGCCGGGCCGGACCGACGTCCGCGATGGCGCGCAGCTCCATGAACCAGCCACCGCCCACCCGG GCATCGGCTATCGGGTGTTCACGGGGGCCGACCCGCGGCGGATCCGCGCGATCCTCACCGAGGTCGTGGGCCTCATG GAGCGGGGCGTGCTGCGTCATCTGCCCCTACGGACCTGGGATCTGCGCCTCGCCCCCGACGCGTTCCGGTATCTGAG CCGGGCCCGGCACACCGGCAAGGCCGTGCTGACACTCCCGGCGCCCCTCGACCCCGAGGGCACCGTGCTGATCGTCG GCGGCACCGGCACGATCGGCGGCATGCTCGCCCGCCACCTGGTCACCACCCACGGCGTTCGCCACCTCCTGGTGACC GGCCGGCGGGGTGCCGAGGCGCCCGGCGCGGACGCGCTGCGCGAGGAACTGTCCGCTCTGGGGGCGGAGGTGACCAT CGCCGCCTGCGATGCCGCCGACCGCGACGCCCTGGCGGCGCTGCTCGCGGACATCCCCGCCGAGCACCCGCTGACCG GCGTGATCCACGCCGCCGGTGTGGTGGACGACGGCACCGTCCCCTCCCTCACGCCCGAGCAACTGCACACCGTGCTG CGGCCCAAGGTGGACGCGGCGGTCAACCTGCACCAGCTGATCCGTGAAGACGATCTCGCGGCGTTCGTGATGTTCTC CTCGACGGGCGGCCCGGGCGGCGCCGCGGGCCAGGGCAACTACGCGGCGTCGAACGCCTTCCTCGACGCCCTCGCCC AGGACCGCCGGGCCGCCGGGCTGCCCGGTCAGTCGCTGGCCTGGGGCCACTGGGAGCAGAGCAGCGGCATCACCGGC ACCCTGGGCGAGCGCGACATCGCCCGCATGGAGCGCTCCGGTGTGCGTGCGCTCTCCTCCGACCAGGGTCTCGCCCT GTTCGACGCGGCCGGGCGCCGGCCCGAGAGCCTGCTGATGCCCGCCCGCCTCGACCCGGAGGTGCTGCGGGACCTCG CCGGCGCCCAGGTCCTGCCCCGCGTCCTCAGCGGCCTGGTGCGGGCGGCACCCGCCCGGCGCGCCACCGCGGCGGCG CAGTACGCGGCCGGGGACGCCCAGCCGATGGCGGAGCGCCTGGCGGGCATGTCCGCCGCCGAGCAGGGCCGCACCCT GCTGGAACTGGTGCGCCGCAACGTGGCGGCGGTGCTCGGGCTCGGCGACGTCCTCGCCGTCGACCCGGCGCGGCCCT TCAAGGAGATCGGCTTCGATTCGCTGACCGCGGTCGAGCTGCGCAACCGGCTCAGCCAGGCCACCGACACCAAGCTG CCCTCCACTCTCGTCTTCGACATCCCCACCCCGGCGCTGCTCGCCGAGCACCTGCGCGAACAGCTCGTCTCCGAGGG GCTGAGCGGGGCGGAGGCCCTCGTTCAGGAACTGGACCGGCTGGAGGAACACGTCGACCTGCTCGCGGACGACACGG AACGGGACACCGTGACAGCCCGGCTCGAGGCGCTGCTGACACGGTGCCGCCAGAAACCCGCGGCGGAGAGCAACGGC GTCGCGGAGCGGCTCCAGGACGCCTCGGCCGACGAAGTCCTGCAATTCATCGACTCCCACCTGGGAAGGGCCTGAAA GAGGACCGCCATGGCGAACCAGGAACAGCTCGTCGACTACCTCAAACGAGTGGCGACCGATCTGCATGACGCTCAGC AGCGCCTCCGCGAGGTGGAGGCCCGGGACCGCCAGCCGATCGCGATCGTCGGCATGGCCTGCCGGTTCCCTGGTGGA GCGGACACTCCGGAGGCCCTGTGGGAGCTGGTGTCCGCGGGCCGTGACGCGGTCTCCGCCATGCCGGATGACCGGGG CTGGGACCCCGGCATGTTCAACGACTCCGGCGAGACGGGCACCAGCTATGTGCGCGAGGGCGGATTCGTCCGCGGCA TGGCCGACTTCGACGCCGACTTCTTCGACATCTCGCCGCGCGAGGCGCTCGCGATGGACCCGCAGCAGCGCCTGCTG CTGGAGACCGCCTGGGAAGCCATCGAGCGCGCCCGCATCGACGTGACCTCCCTGCGCGCCACCAGGACCGGCGTCTT CATCGGCGGATCCACCATCTACTACGCCGGCACCGGTGCCGGCGCGCCGCAGGACGTCGCCGGGTACATGGCCACCG GGCTGGCCGCGAGCTCGATGTCGGGCCGCATCTCCTACACCTTCGGCTTCGAGGGCCCCTCCTTCACGGTGGACACC GCCTGCTCGTCGTCCGGGGTGGCCCTGCATCTGGCGGTGCAGTCGCTGCGCAAGGGTGAGAGCTCGCTGGCGCTGGC CGGAGGGGTGTGCGTGATGGCGACACCCGGCACCTTCCTGGAGTTCAGCAAGTTGAACGGACTGGCCGCCGACGGCC GGTGCAAGGCGTTCGCGGCGTCGGCCGACGGCTTCGGGCCCGGCGAGGGCGTGGGCGTCCTGCTGGTGGAGCGGCTG GCGGACGCCCAGCGCCTCGGACACCCGGTGCTCGCCGTGATCCGCGGCTCCGCGATCAACCAGGACGGCGCATCCAA CGGCCTGACCGCGCCCCACGGCCCCGCCCAGGAACGCGTCATCCGCGCCGCGCTGGCCGACGCCCAGCTGTCCGCCC GGGACATCGACGTGGTGGAGGCGCACGGCACCGGCACCTCGCTGGGCGACCCCATCGAGGCACAGGCGCTGATCGCC GCGTACGGCAGAAGGCGCGGCGAAGGCGCTCCGCTGTGGCTCGGCTCGGTCAAGTCCAACATCGGGCACACCCAGGC CGCCGCCGGCATGGCCGGGGTGATCAAGATGGTGCAGGCGCTGCGGCACGGCGTCCTGCCGA GCACCCTGCACGTGGACGAACCCACCCATCAGGTGGACTGGTCCGAGGGCACCGTGAAGGTGCTGACCGAGGCGCGG CCGTGGCCCGGGACCGGCGGCCCGCGCCGGGCCGGGGTCTCCTCCTTCGGCATGAGCGGCACCAACGCGCATGTCAT CCTCGAAGAAGCCCCCGAGGCCTCCAACGCGGCGGAGCCCGCCCCCGCCACCGAGCTGCCGGTGGTCCCGTGGCTGG TGTCCGGGCGCGGCGAGGCGGCGCTGCGCGCCCAGGCCGCCCGGCTGCGCGACTTCCTCACCGAGCGGCCGGAAGCC GGCCCGACCCGCGTCGGGCTCGCCCTGGCCGGTTCGCGCGCCGCCCAGTCGCACCGGGCCGCCGTGGTCGCCGCCGA CCGCGACGCCCTGCTGGCCGGGCTGGCCTCCCTGGCCGACGGGGTCCCCGCCGGCCATGTGGTCACGGGTGCGCCCA GCCCCGGCGAGGCGGTGTTCGTCTTCCCCGGCCAGGGCTCGCAGTGGGCCGGCATGGCCCTCGACCTGGCCGACGCC TCCCCGGTGTTCGCCGAACACTTCCGCCGCTGCGCGCAGGCCGTGGAGCGGCACACCGAGTACACCGTGGAGTCGGT GCTCAGAGGCGAGCCGGGAGCCCCCTCCCTGGAGCGGGTGGACGTGGTCCAGCCCGTGCTGTGGGTGGTGATGGTGG CGCTGGCCGAGCTGTGGCGGTCCTACGGCGTGGAGCCCGCGGCCGTGGTGGGCCACTCCCAGGGCGAGATCGCGGCG GCCTGCGTCGCCGGCGTGCTGTCCGTCGAGGACGCGGCCCGCGTCGTGGCGCTGCGCTCCCAGGCGCTGCCCGAGCT GTCCGGCCGCGGCGGCATGGCCTCCGTGGCGCTCCCCGTCCAGCAGGTCGGGAAACACCTGGAGGGCTGGGACGGCC GGCTGGGCGTGGCCGCCGTCAACGGCCCCTCCTCGACCGTGGTCTCGGGAGACGCCGACGCACTGGAGGAGCTGCTG GAGACCTGTGAGGCCGACGGGGTGCGCGCCCGGCGCGTCCCCGTGGACTACGCCTCGCACTGCGCCCACGTCGACGC GCTGCGCGACCGGCTGGCCGAGGACCTCGCGGGCATCGCGCCCAAGCCGGGCTCCGTCCCGCTGTACTCGACCGTCA CCGGGCAGCGCATCGACGGCACCGCCATGGACGCCGGGTACTGGTTCACCAACCTCCGTCAGCAGGTGAGGTTCGAA GAGGCCACCCGGGCGCTCCTCGACGACGGACACAGCGTGTTCATCGAGTGCAGCCCGCACCCGGTCCTCGTCATGGG CGCGCAGGAGACCGTGGACGAGGCGGGCGTCAACGCCACGGCCCTCGGCACCCTGCGCCGGGACGAGGGCGGCTGGG ACCGCTTCCTCCTCGCGCTGGCCCAGGCCCATGTCCATGGCGTCGAGGTGGCCTGGGGCCGGGTGTTCCCGGACGGC ACCGGCCCCGCCGACCTGCCCACCTACGCGTTCCAGCGGCGGCGCTACTGGCTGGACGCCACCGACGGCGGAGCCGG TGACCCCGACCGGCTCGGCCTCGCCGCGGCCGGCCATCCGCTGCTGGGGGCCGTCACCGTCCTCGCCGAAGGGGACG AGGTGGTGCTGACCGGCCGGCTCGGCCTGGACACCCATCCGTGGCTGGCCGACCACGCGGTGGCCGGCGCGGTGCTC GTGCCCGGCGCCCTGTTCGTGGAGCTGGCCGTCAGGGCCGGTGACGAGGTGGGCTGCGACTGGCTCGAGGAACTGGT GCTGGCGAGCCCGCTGGTCCTGCCCGACCAGGGCGGCACCCACCTTCAGCTCGTGGTGGGCCGCCCCGACGGTGAAG GCCGCCGCACCCTGAACGTGTACGCACGCCCGGCCGACGAGTCCGGCTCGCCATGGCTGCGCCACGCCACCGGCACC CTGGCGCCCGGCCCGGACGCCGAGCCGTACGACCTCGCCCAGTGGCCGCCGGAAGGCACCGAGCCCGTGCCCGTGGA CGGCTGTTACGACCAGCTGGCCGAGGCCGGCTACCGCTACGGCCCCGCCTTCCGCTGCCTGCGCGCCGTGTACCGCC GCGGGACGGAGGTCTTCGCCGAGGTCGTCCTCCCGGAGGAGCAGCGCGGGCAGGCCGCCGCGTTCGGTGTCCACCCC GCGCTGCTGGACGGTGCGCTCCAGGCCAGTGGGTTGCCCGAAGTACGCGGTGGCCAGGGCCGGATGGCGCTCCCGTT CGCCTGGAACGGAGTGTCCCTCGCCGCCACCGGCGCCGAGCTGCTGCGCGTCAGGCTCGCGCCGGTCGGCGACGACG GCATGTCCGTCCACGCCACCGACGCCTCGGGCCGCCCGGTGATCTCGATCGACGCGCTGGTGACCCGGCCGTTCACC CCCGAGCAACTCCAGGGCGGCGGCGAGGACATCGGCGACGCGCTCTTCCGCGTGACGTACACCCCCCTGACCGGTGC CCCGGCGGGCGGCGCCATCGACGGCTGGGCCGCCGTCGCCACCGGCACCACACCCACCTGCTACGGAGCGCCCCGCT ACGACGATCTGGACGCGCTCGCCGCCGCGGTGGACGGCGGCCTGCGCGTCCCGCCGGTGACACTGCTGCCCTGCGAG CCGCGGCCGGACGACACCGACCTCCCCGGCGCGGTGCGCCACCGGCTGGGCGAGGTGCTGCACTCCGTGCAGCGGTG GCTGGCCGACGAGCGGTTCGCCGACTCCCGGCTGGTGGTGGTCACCCGGGGCGCGGTCGCCGCCTCCGACGGCGAGG ACGTCACCGACCTCGCGCACGCCCCGGTGTGGGGCCTGATCAGGTCCGCGCAGTCCGAACACCCGGACCGGCTGGTC CTGGTGGACCTGGACGGACCCGCGCTGCCCGGGGCCCTTGCCCCGGCGGTCGCCGCGGGCGAGCAGCGGATCGTCGT CCGGAACGGGACGGTACGGGTCTCCGCCCTGGTCCCCGCGGCGTCCGGCGCCGCCCTGACCCTGCCGGAGGACCCCC ACTGGCGGCTGGACATCACCGGCCCCGCCACCCTGGACAACCTGGCCCTGGTGGCGGCGGAGGAGCCCCCGCCGCTC GCCCCGGGCCGGATCCGGGTCCGGGTCCGCGCCGCGGGCGTCAACTTCCGGGACGTGCTGATCGCGCTCGGGATGTA CCCGGGCGACGCGGTGATCCGGGGCAGCGAGGGCGCGGGCGTGGTGGTCGAGGTGGCCCCGGACGTGACGTCGGTGG CCGTCGGCGATCGCGTGATGGGCATGTTCCAGGGGGCGTTCGGCTCGACCGCCGTGGCGGACGCCCGCTCCGCCGTG CCCGTCCCGCCCGGCTGGACCGACGAGCAGGCGGCGGCCGTTCCCATCGCGTTCCTGACCGCCTGGCACGGGCTGGT GGACCTGGCCGGTCTGAAGGCGGGCGAGTCGGTCCTCGTCCACGCGGCGGCGGGCGGCGTGGGCATGGCCGCCGTGC AGATCGCCCGCCACCTGGGCGCCGAGGTGTACGCCACCGCGAGCCCCGCCAAACACCACG TACTGGAGGCCATGGGCATCGACGCCGCCCACCGCGCCTCCTCTCGCGACCTGGACTTCGAGGGCGCCTTCCCGAAG GTCGACGTGGTCCTCAACAGCCTGGCCGGCGAGTTCACGGACGCCTCGCTGCGCCTGCTGGCCGACGGCGGCCGGTT CGTGGAGCTGGGCCTGACCGACGTCCGCGACCCCGAGCAGCTCGCCGAAACGCACCGCGGCGCCCGCTACCGCGTCC TCGACCTCGGGGCACCGAGAGAGGAAGGACTCGGCCGGATCCTGACCGAGGTGCTCGCCCGGTTCGCGCGCGACGAG CTGACCCACCCTCCGGTGCGCGTTTTCGACATCCGCCGGGCGCGCGACGCGTTCCGCCTGATGAGCCGGGCCGCACA CATCGGGAAGATCGTGCTGACCCTGCCCCGGCCCCTCGAACCGGAGGGCACGGTCCTGGTCACCGGTGGCACCGGTA CCCTGGGCGGCCTGATCGCCCGCCATCTGGTCACCCGACACGGGATACGCCACGTGCTGCTGACCGGCCGCCAGGGG CCGCAGGCGCCCGGCGCCGCCGAACTGCGCGAGGAGCTCACCGCGCTGGGCGCCACCGTGACCGTCGAGGTCTGCGA CATCGGCGACCGCGACGCCGTCGCCGGGCTGCTGGCCGGCGTGCCCGACGCACACCCGCTGACCGGTGTGGTGCACT GCGCCGGCGTCCTCGACGACGGGATGGTCGACTCGCTGACCCCCGAGCGGCTGGCCCGGGTCCTGCGGCCGAAGGCC GACGGCGCGGCGCACCTGCACGAGCTCACCAAGGACGCCGATCTGGCGATGTTCGTCATGTTCTCCTCCATCGTCGG GGTGAACGGCAACCCCTCGCAGGCCAACTACGCCGCCGCGAGCGCCTTCCTGGACGCCCTGGCCCACCACCGCCGGG CCCACGGCCTGCCCGGTCAGTCCCTGGCCTGGGGCATGTGGGAGGAGACCAGCCGGCTCACCGGCCGGCTGGACGAC GGGGACCTGGAGCGCATCCGCCAGGCCGGCATGGACCCGCTGCCCACCGAGCAGGCCCTGGCGCTGTTCGACCGGTC GTACGCCGCGGGTGACGCGCTGCTGGTTCCGATCCGGCAGGCCACGGCGGCCTCCGGCAGCCGCCCGGCCGCCGGCC GTGCCAGGGCGCGGCGCGTGGCCGACAGCGGGGCGGCGGGCACCGGCGGCCCGTCGCTGGCGGAGCGGGTCACCGCA CTGCCGGAGGCGGAACGCGAGGACTACGTCCTGGAGGCGCTGTGCGCGCACATGGCGGCCGTCCTCGGCCACGGCTC GGCCGACGAGATCGCCCACGACCGCACGTTCAAGGAGCTGGGCTTCGACTCGCTGACCGCGGTCGAACTGCGCAACC GGCTCTCGGCCGCGACCGGCCTGCGCCTGCCCGCCACGCTCATCTTCGACTTCGCCCACCCACTGGCCCTCGCCCAG CACCTGCGCGAGCGGATCGCCGCGCCCGCCGACCCGGCCGGGAGCGCACCCACCGACCCGGCCGGGAGCCCGCCTCC GGACCCGCGTGAGAGCCGGGTGCGCGAGGTGCTGGCCGCCGTCCCGCTGCGGCGGCTGGAGGAGTCCGGCCTGCTCG ACGCGCTGCTGCGACTGGGCGAGGAACCCGATCCCACCGACAGCACCGACACCGCCGACACCACCGAAAGCGCAGAC AGTGCGGACAGCGCCGATTTCGCCTCGATGGAGCTGGAAGAACTGGTGGACATGGCCCTGCGCAACGGCGATTCCTG ACGTGTGCCGTCCCCTCGGAGGAATGTCATGACGGTGGCCAACGACAAGATCGTCGAGGCCCTGCGCGCCTCGCTGC GGGAGGCCGAGCGGCTGCGCAAGGAGAACGACCGACTGCGCGCGGCAGCCCGGGAACCCGTCGCGATCATCGGGATG GCCTGCCGCTACCCCGGTGGCGTCGAGTCCCCGGACGACCTGTGGACCCTGCTGACGGAGGAGCGGGACGCGATCAG CGCCTTCCCGCGCGACCGGGGCTGGGACCTGGACGGCCTGTGCGGTCCCGACGCCCACCCGGCGGTGCGCAGCGCGG TCGCGGAGGGGGGATTCCTCGGCGGCGCCGCCGAGTTCGACCCCGGGCCCTTCGGGATCTCGCCGGACGAGGCGTCG GTGATGGACCCCCAGCAGCGGCTGCTGCTGGAGCTTGCGTGGGAGGTGTTCGAACGGGCCGGGATCGACCCGCGGTC GGCCGGCGGCCGGCGCTACGGCGTCTTCGTGGGCACCACCGGACAGGACTACACCCCCCACCTGAAGGACGTCCCCG ACGAGCTGCTCGGCCACCTCGCCTCCGGCGGTTCGAGCGCCGTGCTCTCCGGCCGTATCGCCTCCGTCTTCGGCCTC GAAGGGCCCACGGCCACCCTCGACACCGCCTGCTCCGGCTCTCTGGTGGCGCTGCACCTGGCCTGCCGGGCGCTGCG CGGCGGCGAGTGTTCCATGGCCCTGGCCGGCGGTGTCACCGTGATGTCCAGCCCCGACGCGTTCATCGGCAGCGGCC GGGGGATAGGGCTGCCCGTCGCGGCCCGCTGCCGCTCGTTCGCCGACGGGGCCGACGGGATCGCGTTCGCCGAGGGG GCGGGCGTGGTGCTCCTGGAGCGGCTCTCCGCCGCCCGCGCGCACGGCCACCCGGTGCTCGCCGTCGTCCGTGGCAG CGCGATCGGTCAGGAGGGCGCCACCAACGGCGCGAGCGCCTCCAACGGCCCCGCCCAGCGAAGGCTGATCAGGCAGG CGCTGGCCGACTCGGAGCTCGAGCCGCACGAGGTCGACGCGGTCGAGGGCCAGGGCACCGGCGGCCTGCTCAGTGAC GCGGTCGAGGCGCGGGCGCTGGCGTCCGTCTACGGCGAGGGCCGCCCCGCCGACCGGCCGTTGCCGCTGGGGTCGGT GAAGTCCAACCTCGGGCACACGCAGGGGGCCAGCGGTGTGGCCGGGGTGATCAAAATGGTCCAGGCCATGCGCCATG GCGTGCTGCCGCGCTCCCTGCACACCGAGGTGCCGTCACCGCACATCTCCTGGGAAGAGGGCCGGATCCGGCTGCTC ACCGAGGCCACCCCCTGGCCCGCGACGGACCGGCCGCGGCGGGCCGGGGTGTCCGCGTTCGGATTCGGAGGCACGGA CGCACACGTGATCCTGGAACAGGCACCACCTCCGGACGATCCCCCGCCCCCGCTGCCGGAGGCGGGACGCGGGCCGG CGGCCGCCGGTGTGGTGCTTTGGCCGGTCTCCGGCTGCGACCCCGACGCGCTGCGCGGGCAGGCGACCCGGCTGCTC GCCCACCTCGACCGGCGGCCCGAACTCCGGCCGGCCGACGTCGGCCTGTCGCTCGCGACGTCCCGGGCCGCGCTGAC ACACCGGGCCGTGATCGTCGGCGAGAGCCGCCAGGAGCTGCTGGACGGCCTGCGGGCGCTGGCCGGGGGAAGGGGCT CGCCCCGCGTCGTCCAGGGCTCGGCCGGACGCCGCCACCGGCTCGTCGTCCTGCTCAC CGGCCACGCCCTCGCGCCCGGCACCGGCAAGCAGCTGTACGACGCCTTCCCGGCGTTCGCCGACGCGTTCGACGCGG TGTGTGCGGAGCTGGACGCCCCGGTGGGATCCGAGGCCCGTGACGCGATGCTCACCGGCCCGGCCAGGGACTTCGCG ATCCAGGTGGCGCTGTTCCGGCTGGTCGAGTCGTGGGGGGTACGGCCCGGCGTCGTACGAGGCCACGGCGTCGGCGG GCCGGCCGCCGACCACCTCGGCGGGCGGCTGACACTGCCCGAGGCGTGCGCGGCGCTGACCGGCTCGCCTCACCCGG CGGACACCCCGCGGGACTTCGCCGCCGCGGCCCGCACCCTGGCCCGGGAGGGCACGGCCTATCTCGAACTGGGCGGC CATGAGGTGGCCGAACTCCTCGACGGCGAGGGGCTGCCCGCGGTGGCCGCGCTGCGGCCCGGAGCGTGGGAGGTGGC CGCCGCGGCCGCCGCCCTCGCCCATGTGCACGCCCACGGCACCGCCGTGGACTGGCAGGCGGTGTTCGCGGGCGCCG ACGCCCGCCGGGTCGACCTGCCCACGTACGCCTTCCGGCGCGCACGCTACTGGCGCGCGGCGTTCGACCTGAGCCGG GTCAACGGGGCGCGGTGAGACCGCCCGGAGCGGGGTGAGGGGCCGCACCGACCGGTACGGCCCCTCACCCCGCTCGG ACGGCTCTCAGGCTTCGGCGCCCAGCTTCACCGGCAGCCGACCGAGCTGGCGAGTGCCCGGAACGGGGCGCCACGTC AGCTCCTTCTCCGGCACGGCCAGGGTCAGCTCCGGGAAGCGGCCGAAGAGCGTCTCCAGCGCCAGCTCCGCCTCCTG GTTGGCCAGCATGGCTCCCAGGCAGTAGTGCGGTCCGCGTGAATAGGCGACGTGCTGCGCGCCCCCACCGCCGGAAG AGCGGGTGATGTCCAGCCGCTCGGGGTCGGGGAAGCGCCGCGGATCGTGGTTGGCGGCGCCCTGCACGGGCAGGACG ACCTCGCCCTTGCCGATCGGCGCGCCCGCGACCACGACATCCTCCGTGGCGTACCGCGGCTTGCCGAAGACGATCGG ACTGCACCGGCGCAGCAGTTCCTGCACGGCTCCGGGCAGCAGCTCCGGCCGGCTCCGCAGCAGCGCCCGCTGGTCGG GGTGGGTCAGCAGCGCGAGGACGCCGTTGCCGATGAGACCGGCGGTCGTCTCGTGCCCGGCGACCACCAGGTGGAAC AGCATGGTGACCAGTTCCGTGTCCGACAGCCGCCCGCTGTCCTCGCTCCGCACCTGAATGAGCGCGGTGATCAGGTC GCCGGCCGGCTCGGCGCCGCGCCGCTCGACCAGCGAGCGGAGGTAGGCGGTCGACTCCGAGAGCACGCGGCTCAGCG AGAGGGCGTCGGTGTAACCGTGGCTCCACTCGCGCCACATCGCCCTGTCCTCGGCCGGCACGCCGATCAGCTCGCAG ATGACCGCGATGGGCAGACGGTGGGCGAAGTGCTCCATCAGGTCGACCGTGCCGTCCACGGCGCGGCCCGGCAGCCC GTCGACCAGCTCGTCCGTCAACGCCCGTATCCGCGGCTTCAAGTCGGCTACCCGCCGCGCCGAGAACGCCCGCGCGA CGAGCGTGCGCAGCCGCGTGTGATGCGGCGGATCGAGCTGTGTGATGGCCTCGGCGAGATGGGGAACCAGGTCCTTG GACAACCCCAGCCAGCGCAGCGACTCGGCGTGGTGATCCGTCGTACATCCCGGAATCGACCGCGAGTTGCTGACGAA GCGCTCGTCGGTGAGCACGGAGACCACGTCCTCGTAGCGGGTGATCACCCACGCGTTCCGGCCGTCCGCCACGCGCG CCAGGGCGACCGGCGCCTCCTCCCTGATCCGCCCGAAGCCGCCGTACGGATCGGCGGTCAGCTCGGCCGCGTTGATG GCGAACGGGCAGCGGTCCGGCTCACCGGGCGTCTCGGCGTCACCTGCTGGTGTCATCTTCGGGCCTTCCGTCGTCGG CCATCCCTGCGGGTGATGATCGCCCATCCCGCTTAAGCAGTCCTGTCACCGGGGCGGGACCCGGGGCGGGACCCGAG GCGGGGCGCGGGCCGGTTCGGTAGGTCCGCTTAAGAGCGCTCGGCGAGCATGAGCCCTTGCCATTCCCGTTCCGAAA TCACCGCTCCGTTGGTCTCAGCGATGCGGGGAAGCAGGGAGAACACGGTGATCAACGAGCGCAACCGCAAGTCGATC GCACGCGAACACAGCCGGCGCCTGAGTGCGGGCGATGTCGACGCGCTCCTGGAGCTGTACGCGAAGGACGCCACGTT CGAGGACCCCGTGGGGGCCGGCGGCCGGGCCGGACACGAGGCGCTGCGCGCGCACTTCGAGAGCGCGGTGGCCGGGA ACGTCGCCGAGACCGTCGAGGATACGGTGGTCGGGCAGGACGGCGCGCATGTGCTGTCCCGGATCACCGCGGTCATG GACTACCGGCCGCGCGGCCCGGTGTACGCCGACCGCGGCTGGCTGCCCGCGCCGCAGGCCGCGGAGCCGACCGGACT GCGCTGCCACTACGCGCTGCTGCTCCGCGTCGGCGAGTCCGGCCTGATCGAGGACATGCGGGCGTTCTGGGGCCGGC CCGACATCGAGACATCGGCCGACGGCGCGGCGGAGTCCTTCCGCGGTCCCGAGTCCATCACCCCCGACGAGGCGGCT CTGCGGCAGCTGCCGTACACCTACGCCCGGCGGCTGGAGTCGGGTGATGTGTCCGGGACCGTCGCCCTGTTCTCGGA CGATGTCGTCTTCGAGGACCCGGTGGGGCGCATGAAGCTGTGCGGCAAGGAAGCCCTGGCCAAGCACGTGGCCCACG GCTCGGCGGGCAAGGTGAACGAGATCCTGGGACGGCCCGTGTCCTCGATGGACGGACGGTTCGTGGTGATCCGGGGG GACGCCCGGATCTTTGTGCCGGAGAAGATGCGCATGCGCATGATCACCGTCTGTGAACTGAACAAGGACGGCCTGGG CGTGCACATCCAGGGCTTCTGGGGGATCACGGACACCACCTTCGGGGACATGGGGCCGGACACGGACACGGACACCG ACCGCTGACCGGCGCCCGACGCCCGGCGCCGAGGAGCGGCCGGAAACGGCAAGACGCACTTAAGCCGCTTGGGACAC CCTCGTGACGGTGTCCGAGGCGAAAGACGAATGCCACATCAGGCGAGCCCGTTTCGGAGGGACTTCCGCGCCATGAC CACACCGACACGTGCCGTCGTCCTGGGCGGCGGATGGGCCGGCATGCTGACCGCCCATGTGCTGGCCCGCCATCTGG AATCCGTGACCGTGGTGGAGCGTGACCTCCTCCCCGACGGACCCCTCCACCGTAAGGGTCAACCACAGGCCCGCCAC GTCCACATACTCTGGTCCAGCGGCGCGGGCATCGTCGAGAACCTGCTGCCGGGCACCATCGAGCGGCTGCTGGCGGC CGGCGCCCGCCAGATCGGCTGGAACCGCGACCTGGTGACCCTGACCGCCTCG GGCTGGCAGTACCGCTTCCCGGCCACCACCTACGCCATGATGTGCACGCGTCCCCTGCTCGACTGGGTGGTGCGTGA CGCGATCCTCTCCGGTGGCCGCATCGAGGTGAAGCAGGGCACCGAGGTCGTCGGCCTGGTCGGAGACCGCTCCAAGG TCTCCGGAGTGCTGGTGCGTGACGTGGCCGGCGGCGAGCCCCGGCCGCTGGAGGCGGACCTGGTGGTGGACGCCACC GGGCGGGCCTCGCGCCTGGGCCGCTGGCTGGCGGGGCTCGGCCTGTCGGCCGTCGAGCAGGACGTGGTCGACGCCGG CGTCGGCTACGCGACCCGCATGTTCAAGGCGCCCGAAGGCGTGAGCGGCACTTTCCCCGCGGTCATGGTCGCCGCCG ACCACCGCACCCGCAGCCCCGGCCGCTTCGGCGTGGTCTACCCCCAGGAGGGCGGCCGCTGGCTGGTCACCCTCACC AGCACGCGTGGCGCCCCGCTGCCCACCCACGAGGACGAGTTCACGGGCTTCGCCAAGATGCTGCGGCACCCGATCGT CAGCGACCTCATCGGCGTGGCCGAGCCCATCAGCCCGATCTTCCCCTCGCACTCGGGAGCCAACCAGCGCCTCTACC CCGAGCGCATGGCGCAGTGGCCGCAGGGCCTGCTGGTCATCGGCGACTCGCTGGCGGCGTTCAACCCCGTCTACGGC CACGGCATGAGCACGGCCGCGCGCGCCGCCGAGGCCCTGGACAGGCAGCTGGCGGGCGACGACTTCGGCGAGGGCGG CACCCAGCGGGTCCAGCGGGCGCTGAGCGAGGTGGTCGACGACCCGTGGATCATGGCCGGGCTGAAGGACATCCAGT ACGTCAACTGCCGCATCCGCACCGACGACCCGCGCCTGACCGACCCGGACACCCCCGAGCGGCTGAGGTTCTCCGAC TACGTCGCCGACCGGTCGATCCGCTCGCCGAAGGTCAGCGAGGTCGCCACCAGCGTGCTCAGCCTGAACACGCCGCA GAAGGCCCTGGGGGACTCCCACTTCCTGTCGCTGCTGCGGACCGACACCAAGTACCCGGAGCTCGTCGAACCGCCGT TCCACCCGGACGAACTGGAGATGGTGGGGCTCAAGCCGCCCGGCAACGCCGCGAACGGCGTGCTCGGATAACCCACC CCGCAGGCCCCACATCGAGAGAGGTACAGCCATGGAACCCATCGAAGAGTCGACCGGCGACAGCCGACTGGCCGAGC GGCTCGCGACCGCGACGCCGGAGGAGCGCGAGCAGCTCCTGCGCGGGCTGGTGCTCCGCCACGCCGCCGAGATCCTC GAAGCTCCGGAACTTGACGAGGAGAGCAACTTCGTGGAGAACGGCCTGACCTCGCTGCGGGCCCTCCAGCTGTCCAA GGCCCTGATGAACGACACCGGGCTCGAAGCCCCCCTCGTCGCGATCGTCGAGCACCCCACGCCCACCCTGCTCGGCA AGTTCCTGTCGGAGACCTACGAAGCGGACGCGGCCTAGGCCTGCACCCGTCTTGTCCTATCTCCAGGAGGCAGGGGA TTTCCGCCATGAAGCTGTCCGTTGCCGTGGACCACGGAGTGACGTTGCGGGTGCGGCACCGGCCGGGCGGCGACGGC CGGCCGTTCCTGCTGCTCCACGGGCTGAGGTCCAACGCCCGGATGTGGGACGACGTGGCCGACCGGCTGGTGGCCGC CGGCCATCCCGTCTACGCGCTCGACATGCGCGGGCATGGTGACTCCGACCTGCCGGAACACGGATACGACAACGCGA CCGCCACGGCGGATCTGGTCGCCGTCTGCCGCGAGCTGCGGCTGACCGGGGCGCTGCTCGCCGGTCACTCCTGGGGC GGGAACCTCGCGGTGCGGCTCGTGGCCGAACACCCCGAGGTGGCCGCCGGTCTCGCGCTGGTGGACGGCGGCTGGAT CAGCATGGTGGACACCTCTCAGGGCGGCCCCGCCCGGGAGAAGGTCGTGGAGATGGTCGGGCGGGCACACTCCCAGG TTTCCGGGATGACCGCCCAGACCCTGCGGGAAACCCTGCGCGGCCTCCACCCGACCTGGTCAGCTGCCGCCGTCGAG GCGACCCTCGCCGACCTGGCCGAAGGGCCGGACGGGCTGCTCGTCCCGCGGCTGCCGCTGGAGAAGCTGCTGTCGAT CCTGGAGAGCATGTGGGAGGACCCGCCCGCCCGCTGGTATCCCGCCATCACGGTGCCCGTGATGCTGTGCAGCACCC TGCCCGTGCAGGTCCCCATGCTGAGCGACCTGGCGCGGAAGTGGGTGGCGGAGGTCGAGGCGGCCCTCCCGCAGGCG GACCCCCGCTGGTACGTGGGCGCCGACCACAACGTGCACGCGGATCACCCCGCGCGGGTGGCCGGCGACCTGCTCGA CCTGGCGGCCACCGTGGACAAGCCGGCATCGGAAAGGCCCTGACATGGCGAATGACGAGACGAAGCTCCTCGGCTAC CTCAAGCGCGTGACGGCCGACCTGCGCGAGGCCCAGCGCCGGCTCAAGGACGTCGAGTACGCCCAGCACGAGCCCGT CGCGATCATCGGCATCGGCTGCCGGTTCCCCGGCGGGGTGAGCTCCCCCGACGACCTGTGGGACCTGGTCGCGGCCG GCCGCGAGGGCATCGGGGGCCTGCCCACCGACCGCGGCTGGGATCTCGAAGCGCTCTACGATCCCGACCCCGACCAC GCGGGCACCAGCTATGTGCGCGGGGGCGGATTTCTCTACGGCGCCGACCGGTTCGACGCCGGATTCTTCGGGATCGG TCCGCGCGAGGCGGTCACGATGGCCCCGCAGCAGCGGCTGGCGCTGGAGGTGGCCTGGGAGGCCATCGAGCACGCCC GCATCGACCCGCACACCTTGCAGTCCAGCGCCACCGGTACCTACCTCGGCTGCGACGGCCTCGACTACTTCCTCAAC TCCCTCCAGGTGCCTGAGGGCTCGGCGGCCCATCTGACGACCGGCAACTCGCCGAGCGTGGTGGCCGGTCGGGTCGC GTACACCCTCGGCCTGGAAGGCGCCGCGGTCACGCTGGACACCGCATGTTCCTCGTCCCTGGTCGCGATCCACCTGG CGTGCCAGGCGCTCCGGCAGGGGGAGGTGACGCTGGCGCTGGCCGGCGGGGTGTGCGTGATGTCCTCACCGGCACCG CTGGTCGGCTTCAGCGAGATGCGCGCCGTGGCGCCGGACGGGCGCTCGAAGCCCTTCTCCGCGGACGCCGACGGCAT GACCCTCGCGGAGGGGGCCGGGGTCGTCCTGCTGGAGCGCCTGTCGGACGCCCGCCGCAACGGGCACCGGGTGCTGG CGGTCGTCCGCGGCACCGCCGTCAACGAGGACGGCGCCAGCAACGGTCTGACGGCGCCGAACGGCCCGTCGCAGCAG CGCGTCATCCAGCAGGCGCTGGCCAACGCCCGCCTCGCGGCGGCGGATGTGGACGCGGTCGAGGCGCACGGGACGGG CACCAGCCTCGGCGACCCCATCGAGGCCCAGGCGTTGCTGGCCACCTATGGGC ACGACCGGCCGGAGGGGCGGCCGCTGCGGCTGGGGTCGATCAAGTCCAACATCGGCCACACCCAGATGGCCGCCGGT GCCGCGGGCGTGATCAAGATGGTCATGGCGCTGCGCCACGAGCTGCTGCCGCGCTCCCTGCACATCGGTGCGCCCAA CCCCCATGTGGACTGGGGCAACGGCGCGCTGAGCCTGCTGCGGGAGGCCGTCGCGTGGCCGCGCGACGCGCGGCCCC GGAGGGCCGGCGTGTCCTCCTTCGGATTCTCGGGGACCAACGCGCACCTGATCCTGGAGGAGGCGCCCGAACCTCCC CCTGGGGACCCCGGCGAGCCCGGCGAGCCCGGCGAGGCCGCCGATCGGCCCGAGGGCCGGGTCACGCCGTGGGTGGT GTCGGCACGCGGCGCGAAGGCGCTGCGCGGCCAGGCCCGGCGGCTGCTCGACGCGACGGCCGTCGTCGGCGGCGAGC CGGAGGCGGTCGGCTGGTCGCTGGCCACCACGCGCGCGACGCTCGCCGACCGGGCGGTGATCACGGGCCGGGACGCC GACACCCTGCGGGCGGGCCTGGCCGCGCTGGCGGCCGGGGAGGACCATCCGGCACTGGTACGGACCGCCGCCGCGGC GCCCGCCCCCGGGTGGCGGGTGTTCCTCTTCTCCGGGCAGGGAAGCCAGCGGCCGGGTATGGGGGCCGGACTGTACG AGCGTTTCCCGGTGTTCGCCGCCGCCTTCGACGAGGTGTGCGCGCTGCTCGATCCGCACCTGGACCATCCGCTGCGC GACATCGTCCTCGCCACCGACGAGGAAACCGCCGGCCTCGTCCACCACACCACCTATACGCAGACCGGGCTGTTCGC GGTGCAGGTGGCGCTGGCACGGCTGCTGCACGACTGCGGTGTGCGGCCCGACGCCGTGCTCGGCCACTCCATCGGCG AGATCGCCGCCGCACACGTGGCCGGAGTGTTCTCCCTGGAGGACGCCTGCCGTCTGGTCGCCGCCCGCGCCACCGCG CTCGGCGCCCTGCCGCCCGGCGGCGCCATGACCGCCCTGGAAGCCACCGCCGAGGAGGCCGCCGAAGCCCTCGCCGG CTACGACGGCCAGGTCGGCATCGCCGCCCTCAACGCCCCCACCAGCACGGTCATCTCGGGGCCGGCGCAGCTGGTGG CCGAGGTCGGCGCGGCCTGGAAGAAGCGCGGCCGGAAGGCCAAGGCGCTCACCGTCAGCCACGCCTTCCACTCCTCC CTCATGGAACCGGCCCTCGACGGCTTCCGCGACGCCATCGCCGACCTGGCCTTCCACCCACCGCTCATCCCCCTGAT CAGCAATCTGACCGGGCAGCCGGCCGGCGAGGACATCGCCACCCCCGACTACTGGGTGCGCCACATCCGCCAGCCCG TCCACTTCCACCCCGCCGTCGCCCACGCCGCCCCGCAGACCGCCGTCTACCTCGAAATCGGCCCCGACCCTGTCCTC ATCCCTCCCGCCCAGGACACCCTCGACACCCTGCGGGACGCCCCTGGGGCCACCGCGCCGCTGCTGATCCCGACGCT CACCCGCACCCAGCCCGACACCCAGGCCCTGGCCCAAGCCCTGGCCCGGCTCCACACCCACACCCCCGTCGACTGGA GCCCCTGGTTCACCCACCCGGCCCCGCCGCCCACCGTCGACCTGCCCACCTACTCCTTCCAGCGCGACCGCTACTGG CTGGCCCCGGCCCGTCCGACCGGTGGCCACGGCGTGCAGGATCCGGCCGAGGCCGAGCTGTGGGAAGCCATCGAGGA CCACGACGTCGAGACTCTCGCGCAGGTGCTGGAGCCCGACGGCGAAGCCGAAGACATCGAGGCGCTGCGGCCGGCCC TGCCCGTGCTGTCCGCCTGGCGCCGCCGCCACCGCGAGCGCGCCACCCTGGCGTCGTGGCTCCACCGGATCCGGTGG ACGCGGCTGCCCGAGCCCGACGCGCCGGCGCTGAGCGGCACCTGGCTGCTGCTCGTCCCCGCCGGCCACGAGAACCA CCCGGCCGTACGGACGGCGCTCCAGGCCCTGACCGAACACGGCGCCGACGCCACCACCTGTCCCGTCGATACCGCCA CCAGCCGTCAGGCGCTCGCCGAACGCCTCACCGCCCTGCGCTCCGAAGCCACGCCGTCGACCGCCCCCGCCGGCGTG CTCAGCCTGCTGGCCCTCGACGAAGCCCCGCACCCCGATCATCCGGCCGTGCCCGCCGGGCTCGCCGCCACCCTGAC CGCCCTCCAGGCGCTCGGTGACTCGGACATCACCGCGCCGCTGTGGTGCCTCACCCAGGGGGCGGTGTCCACCGGGC AGGACGACCCACTGCCCCATCCCCTCCAGGCGCAGAGCTGGGGCCTGGGCCGGGTCGCCGCGCTCGAACACTCCCAG CGCTGGGGTGGCCTGGTGGACCTGCCGGTCGACGTCGACCAGCGGACCCCGGCCCGGCTGGCGGCCGTGCTGGCCGG CGCGCCGGCGGGCGCCGAGCCCGAGGACCAGGCGGCGATCCGCACCACCGGGGTGCTGGCGCGCCGCCTCGTCCACA GCACCACCGGGCACGCCGGGCCCGGCCGCGACTGGCGGCCCCGGGGCGCCGTGCTGATCACCGGCGGCACCGGTGGC ATCGGCGCACTGCTGGCCCGCTGGGCGGCCGAACACGGGGCCGCCCACCTGGTCCTCATCAGCCGCCGCGGCCCCGA CGCGCCCGGCGCCGGGGAACTCGCCGCCGAACTCAGCGGCCTGGGCGCCACGGTGACCCTGGCCGCATGCGACGCGG CCGACCGCGAAGCCCTGCGCGCCGTCCTGGACGCGATCCCCGCCGAGCACCCGCTGTCCACCGTCATCCACGCCGCG GGCGTCAGCGAGCACGACGCCGTCGCCGACGCGGACACCGGACACCTGAACCGGCTGCTCGAACCGAAGGCGCTCGC GGCCCGGCACCTCCACGAACTGACCCAGGACCTGGACCTCTCCGCGTTCATCCTGTTCTCCTCGGTCTCCGCGGCCT GGGGCAGCGGGGGGCAGAGCGGCTACGCCGCCGCCAACGCCTACCTGGACGCGCTCGCCGACCACCGCCGCGGACTC GGCCTGCCCGCCACCTCGGTGGCCTGGGGGCTCTGGGGCGAGGTGGGCATGGCCACCGCGTCCGACGACGTCGACTA CTTCCGCCGCCGCGGCCTCCACCCCCTCGACCCCCGGCTGGCCGTCGCCGCCCTGGAGCAGGTGGCCGGAGGCAGGG AGACCAGTGCCGTGGTCGCCGCCGTCGACTGGCGGCGCTTCCTGGAGAGTTTCACCGCGCTGCGCCCCAGCCCGCTG CTGGGCGATCTCCCCGAGGCCGCCGAGGCGCGCACGGAGGAACCGCGACCGGCGGCCGAGGAGACGGACCAGCTCAA GCGGAAGCTCTCCGGCAGCGCGCCGGCCGAGCAACACCACATCCTGGTGCGGCACGTACAGGCACATGCCGCCGGTG TCCTCGGCCACGCCGCCGCGGACGACGTCCCGCCCACCAAGCCCTTCCAGGAGCTGGGCT TCGACTCGCTCAACGCCGTCCAGCTGCGCGACCGGCTCAACGCCAGCACCGCGCTGCACCTGCCCACGACCGTGCTG TTCGACTACCCGTCGGCCGACGAACTCGCCCGCCATCTGTACGGCCTGCTCGTCACCGACGGGGCCTCGGGCGAACA GCGGGTCATGTCGGAACTCGACGGCTGGGACGTCGCCCACGCACCGGACGCCGTGGACGAGGCCGCCCGCTCCCGGG TCGCCGCACGGCTGCGGCTGCTGGCCGACAAGTGGGCCGACCCGGGCCGCGCCACCGGGACCCACGACGACCTGGAG ACGGCGACGGCGGAAGAGATCTTCGACCTGATCACCCAGGAGTTCGGGAAATCCTGAGCGGGGCGCGGAAGAAGGGA GGGGTTCGTCAGCGGACTGACGAACCCCTCCCGAGGCTTTGGGCCCAGGGCCTCAGCGGGAGCCGTACTCGGCCGCC AGCTTCTCCAGCTCGGGGACCACGTCATTGGGGGACGGCTGAGCCGCCATCTCCTGACGGAGCCGCTCGGCCCCGGA CCGGAACGACGGGTCCTCCAGCAGCCGGACCAGGTCCTCCCGGATCCTCGGCCCGGTCATGTCCTGGGGCGGAATCC ACAGCCCGGCCCGGGCCTCCTCAAGACGTTGGCCCCGTATCTGGGCGTCCTGAGCGGTGAGGCTGACCATGAGCTGC GGGACTCCGTGCGCCAGTGCGCCGCAGAACGCCGGGACCCCACCGTGGTGCATCACAGCGGAACATGAGGAGATCAC GGCGTGCAGGGGCACGAAATTGACGATTCTCGTGTTCTCGGGGACCCGCTTGAGTTTCCGCTGAATACTTTCGGAGA GCGTCAGCATCAGCTCGATGTCCAGGTCGGCCACCGAGTCCAGCATCTCCTGGATCTGGCCGACGGGAACGGGCTGG CTCCCTGCCACGCTCCACATGCTCACTCCGAAGGTGGCAAGGACGCGCGGCGCCGGCGGCTTCTCGTGAATCCAGTT CGGAATGACGGAAGGGCCGTTGTGCGGGACGTAGCGAACGGAAACGTTCCGCACATCGCTTTCCGACCTTATCGAGT CCGGCATCTGATCGATCGTGAACTGGCCGGTCAGCAGTTCTTCCGAGGCATCAAAGCCGTATTTCGACGCCCACTTG CTCATCCATTCTTCCACCGGGTCCTCGCGGTCCCCCGGCGGCTGCTGCGCCCGCACCGTCAGAAAGCGCTGACGCAA AGGCGTTCCCACGTCGATGGCGAGGTGCATCCGCGCATGCGGCACTCCCGCCGCGGCGGCGGCGACCGAACCGGCGT AGCTCATCGATTCCCAGACCACCAGATCGGGCTTCCACGAGCGGCAGTACGCCACCAGGTCGTCGACCATCGAGTCG TTCGCGGCCTCCATGATCATGGCGAAGACGGTGTGGAACGCCTTCAGGTTCTCCCAGGTGAGCTGCTCCTCGCGGGC GTAGTCGACCTTGGGCAGCACCATGCCGCTCCGCGCTGCCCGCTGGATGATCAAGTCCCCTTTTTCCTGGAGGGACG GACCCGATCCCGCGGCTACCGCCGTCAGCCCTGTTCCGGTGATGACGTCGGTGAATTCGGGGCTGCTCGCGACATGG ACGTCATGGCCGGCGGTGCGCAGCGCCCAGGCCATGGGCACGGTGAGATAGAGGTGGGTTTTCCAGGGGAATGTGAC GAACAGGACGCGCATGGCTACCGTCTCTCCTCACCAGTCGCCGTGGGCAACCGATCTCGCTCGCCTGATCCCAATAT GCTATCGCCGCCCCTTAGGCGGATCTTACGGTCCGCTGTTAGCCGAACTTTAGGCAGGTGAGTGAGGATATGACGGA ACCACAATGAGGTGGTGATATGTCGGCGTCGTCAGGATTCCATGATGAAGGGTATTGTTCTCGCGGGAGGAAATGGG ACACGGCTGCACCCTCTTACCCTGGTGGGGTCGAAGCAGTTGGTTCCCGTTTACGACAAGCCCATGATCTACTATCC GCTGTCCGTTCTCATGTTCGCGGACATCCGGGAGATCCTCGTCATCACCCGCCCCACCGAAATGCCGCTGTTCCAGG AACTCCTCGGAGACGGTGAGCATCTGGGACTGAGCATCAGCTATGCCTCCCAGGAGAAACCGCGGGGGATCGCCGAT GCCTTCCGGGTCGGCGCGGACCACATTCGCGGTGAGGACTGCGCGCTGATTCTGGGCGACAACCTCTTCCACGGCGC GAAACTGCCCACGCTGCTCCGCCGCAGCGCGGAGCGGCTGCGCGGGTGTGTGATGTTCGGGCACGAGGTCGCCGACC CCCGCCACTTCGGCGTCGCCGAGGTCGACGACCGGGGCCGGCTAGTGTCCATCGAGGAGAAGCCGGAGCGGCCCCGG TCGAACCTGGCCATCCCCGGCCTGTACTTCTTCGACGGCGGCGTCGTCGACGTCGCCAAGCGCCTGGTGCCCTCGGC CCGGGGCGAGCTGGAGATCACGGACGTCCTGCGCGCCTACCTGGAGGACGGCCTGGCCGACCTGGTGTGGCTCGGCA GGGGCGTCACCTGGCTGGACACCGGCTCGCACGAGACCCTCCTGGACGCCGGGCGCTTCGTACGCGACCTTCAGCAC CGTCAGGGCACACGGCTCGGCTGCGTCGAGGAGATCGCCATGTACATGGGATTCATCGGGGCAGCCGAGTGCCACGC GCTCGGCGCCAGGATGAGCACATCGCCCTACGGCCAGTACGTCATGGACCGTGCGCTCGCATACCGGCAGGGGCTGC CGCCCGCGCGTTTCCTGGAGGAGCTGTGAACCTCCTCGTCACCGGGGCCGCGGGGTTCATCGGCTCCACCTATGTGC GCGGTCTCCTCGCGCGGGGCGGCGCGGAGCCGGACCCCGACGTGTCCAGCGTCACCGTGCTGGACAAGCTCACCTAC GCCGGGACGCTGAGCAACCTCGAACTCGCCGATCCCCGGCTGACGTTCGTCCACGGCGACATCTGCGACGCCGATCT GGTCGACACGCTGATGGCACGGGCCGGCCAGGTGGTCCACTTCGCCGCCGAATCGCATGTGGACCGCTCCATCGACG GCGCCGACCCGTTCGTACGCACCAACGTCGAGGGCACCCACACGCTGCTTCAGGCCGCCCTGCGCCACCGGGTGGAG CGCTTCGTCCATGTGTCCACCGACGAGGTCTACGGCTCGGTCGAGACCGGCACCAGCCCCGAGACCGCGCCCCTGGA CCCCAACTCTCCCTACGCCGCGTCCAAGGCCGCCTCCGACCTGATCGCCCTCGCCTACCACCGCACCCACGGCCTTG ATGTGCGCATCACCCGGTGCAGCAACAACTACGGCCCCCATCAGTTCCCCGAGAAGATCATCCCGCTGTTCATCACC CATCTGCTCGACGGCGAGGACGTGCCGCTGTACGGCGACGGGCTGAACGTGCGCGACTGGCTGCATGTGGAAGACCA CTGTGGCGGGGTGGAACTGGTGCGTACGAAGGGGCGTCCCGGCGAGATCTACAACATCGGCGGTGGCA CCGCGCTGACCAACCGGGAGCTGACCGGGCGCCTGCTGGACGCCTGCGGAGCCGACTGGAGCCGCGTACGCCACGTC GCGGACCGCAAGGGGCACGACCGGCGCTACGCGGTCGACGACGACAAGGCAAGGAACGAGCTCGGCTACCGCCCGCG CCACGACTTCGTGGCCGGGCTGGCCGAGACGGTCGCCTGGTACCGGCAGCACCGCACGTGGTGGGAGCCGCTCAAGC GCTCCGCCCAGCCCGCACACCGCTGACCTCAACCGACACACCGATGACCGCGCGGACCCCGCGCGGTCAACGGTGTG CGGTGGCGACGCTCAGCGGTGGCCTCCGACGAACTCGGCGACGGAGGCCACGACATAGTCGATCATCTCGTCGGTCA GCCCCGGATAGACCCCGACCCAGAACGTGTGGTCGGTGATGATGTCGCTGTTGGCCAGCTCGCCGCACACCCGGTGC GGCGCCTCGGCGTGCGCGGGATGGCGCGTCAGATTCCCGGCGAACAGCCGGCGGGTGCCGATCTTGCGGGACTCCAG GAAGTCGACGAGCTGGGCGCGGCTGAACGGGGCCCGCGGGTCGACGGTCAGGACGAAACCGAACCAGCTCGGATCGG AGCGCGGCGTCGCCTCCGGCAGGATCAGCCACGGCAGCCCGTGAAGACCCTCGCGCAGCCGCCGCCAGTTGCGCCGC CGGGCCGAGCAGAACGCGTCGAGCTTGTCGAGCTGGGTCAGCCCCAGCGCCGCCTGGAGGTCGGTCGACTTCAGGTT GTAGCCGATGTGGGAGAAGATGTACTTGTGGTCGTAGCCGGCCGGCAGCGTGCCCATCTGGTAGCCGAACCGCTTGC GGCAGGTGTCGCTCTCGCCCGGCTCGCACCAGCAGTCCCGGCCCCAGTCCCGGAGCGACTCCACGATCCGCGCCAGC ACCAGGTTCGAGGTCAGCACGCAACCGCCCTCTCCCGTGGTCAGATGGTGCGCGGGATAGAAGCTGACGGTCGTCAG ATCGCCGAACGTCCCCGCCAGTCGCCCGTCGTAGGTGGACCCCACGGCGTCGCAGGTGTCCTCGATCAGCACCAGGT CGTGGTCCTGCGCGAGCCGCGCCATCTCGGTGGCCTCGAAGGGGTTGCCCAGCGTGTGCGCCATCATGATCGCCCGG GTGCGCGGGCCGATCGCCTCCGCCACCCGCTCGGCCGTGGCGTTGTACGTCCCCAGCTCGACGTCCACATAGACCGG GACCATGCCGTTCTGGAGGATCGGGTTGACGGTGGTCGGGAAGGACGCCGCCACCGTGATGACCTCGTCGCCCGGGC GCAGCCGGCGGTCGCCCAGCAGATGGGAGGTCAGCGCCGAGAGCGCCAGGAGATTCGCGGACGACCCGGAGTTCGTC AGATGCGCCTTGCGCCGCTTCATCCGGCGCGCGAAGGCCGACTCGAACTTCCGCGAGGCCTTGCCCGCGGCGATCCG CATCTCCAGCGCGGCCTCCACCAGCGCGACCCGGTCGTCCTCGTCCAGCACCGCGCCCGAGGGCCAGATCTCGGTGA CCCCGGGGATGAAGCCCCGCCCCTCCTGGCTGTCCTGGTGATACTTGCGCACCTCGTCGAGGATGCGCGCGACTCGT TCGCTCATCTGCTGTCCTTCCCATGGCTGTTCGGGGGTCCGCAGGCCGTCGCCGCCCGCACCGTCGCGGGCGGCGGC ACGCCGGCGGCCGCGAGCAGATCGCGCACCGACTCCCGCAGGGCTCGCCGCGGCGACCAGCCCAGCAGGCGGCGCGC CCGGCCGATGTCCAGTCGTTGCCAGGCCGCGTCCGAACGTGTCCGGTGCAGGGCCGGGTCCTCGACGACGCGCACCG GCAGCCCGCTGAGGGAGATCATCAGGTCGACCAGCGCACGCACCGGCACCGCCCGGCCGCTGCCGATGTTCACGACA CGGCCGGCCACCGCGTGGGACGGTGCCAGGGCCGCCGCGACCACCGCGTCGGCCACGTCCCGGACGTCCACCAGGTC GCGGTGGTCGTGCAGGGGCGCGAGCCGCAACTCGTCGCGCCCGGCCGCCAGGTGCGCGGCCACCACCCCGGCGAGGC TGTTGCCCGGGGCGCCCGGGCCGCAGGCCACGCCGACCCGCAGCACGATCCCGTCCACCCCCAGCTCGGCGGCGGCC CGCAGCACCGCCTGCGACCCGCGCAGCTTGGTGCGGCCGTACACGGTGGTCGGGGCGGTCGGCCAGTCCTCGCCGAT CGACGTGCCCGGGTCGGCCGGGCCGTACTCGTAGGCGCTGCCCAGATGGATCAGACGCGGCCGGTCGGGCAGCCCGG CGACGGCCCCGGCCAGCCGGCCGACCAGTTCGGTGTTGGCCTCGGCCATCCGCCGTTCGCCACCGCCCCACACCGCG CCCGAGGCGTTCACCAGGACCCGCGCCCGGGTGTCGGCGCAGAGCCTGGCCAGCTCGGCCGGCTCGGCCTCGGTCAG GTCGAGACGCACCGAGCGGCAGCCGTCGCCGTCCACGCCGCCGTCCACGCCGCTGCGGGAGACCCGCACCACCCGCG CGCCCGCGGCGCCGAACGCCGAGCAGATGTGCCGTCCCAGATACCCGGACCCGCCCAGGACCAGGACCGGCGTCGTC ATGCCGCTTTTCTGCTGCTTGTTTTCCTGAAGACCGCCTGCCGGAAGTAGCTCCAGGGAACGCCCCGCGCGTCGGCG GTACACGCCCATCCCGCACCGGGCCGATAGGCCTCGATGAAGGCGGGCACGGCCGCGTCCACGGCCTCGCTGTCGTG GATGTCGAGCACCTGCCGCAGCGGCCCGGTCTCCATGGCCAGCCGCACGGCCTCCTCGCCCTTCTGATGGCCGTCCA TTGCCTTGTCCCGGAACTTCCCGACCGGATTCTTGACGAAGAACGCCTCGCACCTGTCGTCGATCAGACGCAGATAG GCCTGCACGGTGTCGGGGGTCATCTCCGTGAAGGAGTGCACATTGACGCACAGCTCGAAGCGGTCCGACCCCAGGGC GGCGTCGATGTCCTCGTCCTCGACCTGGATGAACCTCATCTTGGCGAACTGCTTGTCGTCCAGGACCTCGCGAAGAT ACGCCCTGCTCAGGGAGAGGGTGTTCTTCAGATCGACAATGGTGTAGGAGTCGAGGTCGTAGTTGGACAGCATCGCG TGGCAGGTACGGCCGTATCCCGCGCCGATCTCCAGCACGCGGGCGCCGTCCAGGGTCAGTTCCCTCTCGATGAATCC GAGTTCGAGCGCCGCCTGGAGATAGTCCAGGCAGATGCTCTTGCCCTCGTAGCGAACCGTGACCGGATCTCCCACGT CACGGTTGTGGACATTCTCGATTTTCGACCATTCGTCGTCACTCAGCCGCGTGGCCAGCTCGTAGACGAGGGCCCTC AGATACCTGACCCCGTTGGCCGTCGGGTCCCAAAGAGCGATATTGAAATTCCGTCGGTCCGACTTGAATCCCGTCAA GTCGGCGGCCGCGTCCTCAGTGACCCAGAGCTCGTTGATGCGCTCCCACTGTCGGCTGG CCTGAAAACCCTTCGCCATCCCGCCCTCCTACTCCATCGGCGATGGTCACATCACGCGGTACGAAGTCTGTCTCCCG TGTCTAATGGCGGGCTAACACTTCCGGGTGCCGTGGTCAGGACTCCCCGAATTCCCGGTCGATAAGGGCGAACACCT CATCCGCGGAAGCCGTTTCCAGGTCGTCCCCGCCCGTCTCCTCGCCAGGTTCGGACGCGCTCTCCCCGTCCGTGTAA CGCCACAGCAGCACCTGTAGCCGGTGCGTCAGCCCGGAACGCCGCGGGTCGTCCGCGTCCAGCGCGGCGAGCGCCGC CTCGATCCCGTCGAGGTGCTCCGCGGCCGGTACCTCCGCCGGCTCGGCCGGCAGCACCCGCGACACCAGATGGCCCG CCAGCGCGGTCGGCGTCGGGTAGTCGAAGACCAGCGTGGGCGACAGGGTCAGGCCGGTGGCGTCCCGGAGCCGGTTA CGGAACTCGACGGCCGTGAGGGAGTCGAACCCCAACTCGTTGAAGGGCTGGTCCGGTTCGACCAGCTCCGCCGCCGA ATAGCGCAGCGCGCTCGCCGCGTGGGCGCGGACCAGCGCCAGCAGCGTGCCGGTGCGCGCCTCGGGCTTCTGGGCCG CGAGGCGGTCCCGCAGGGCCGTCAGGCTCCCGGAGTCGTCGGACTCGGGGGAGCCCGGCGCCGCCCGGCCCGCCCGC CGGGCCGCCGGGATCTCGTCGAAGAGGCGGCTGGCCCGCCCCAGGGTGAACAGCGGGGCGAAGCGCTCCCAGTCCAC GTCCGCGAACACCGCGTGGGCCTCGTCGTGGCCGGCCTGATCGAGCGTCCGCCGCAGCGCGGTCAGCGCCAGCGCGG GGTCGAGGGCCGGGAGGCCATGGCGCCCGGAGCGTTCGACCGCCGCCCGCTCCGCCATGTCGTCCCACACGCCCCAC GCCACCCGGACGACGGGCCCACCGGCCGCCTCGCGCCGCCGCGCCACCGCGTCCAGGTATGCGTCGGCCGCGGCCCG GGCGCCGTGCTCGCCGGCCCCCCAGACCGCGACGACGGACGAGAAGACCACCACGGGATCGTCGGGCTCCAGGCCGC ACAGCTCCGGGAGCCGATCGGCGCCGCCCACGGCGGCCGACAAGGACTCGGCCAGCCGGTCCGGGGTGAGTTCGGCC AGCGGTGTCCGGTCACCCGGCGCCCCGGTGTGGATGATCGTGCGGACCCGCAGCCCCGAGGCCGCGAGCCGGTCCGC CAGTTCGTGCCGGCCCGACGGCTCGGACGGCACACCGTCCACCACCGTCGCCTCCGCGCCCAGCTCCGCCAGCTCGG CCACCAGCTCCGCGCCACCGGGCGCGGCCTGGCCGCCCGGCGCCAGCAGCACGATGCGCTTCGTGCCACCCGTGGCC AGCCACCGGGCCACATGGGCGGCGGCCCCGGCGACGCCGCCGGTGACCAGGACGCCCTCCCGCGGCGTCCACTCACG CGTTGCCGCCTCCGCGTCCAGCGGCGCGGGCAGCAGCCGGCGGGCGAAGGCTCCGGCCGGCCGCAGTGCCACCTGGT CCTCGCCGTCCCGCCCCGCCAGCGTGGCGCACAGAAGGCCGGGGACGAGGTCCTCGACCGAGGCGGGCAGGTCCACC AGCCCGCCCCAGGCCGTCGGATGCTCCAGCGCGGCCACCCGGCCGAGCCCCCATACCTGGGCCTGCGCCGGGGCCTG CGGGCGTTCGTCGTCGGCGACAGCCACCGCGCCTCGCGTGGCGCACCACAGGGGTGCGTCGAGCTCCGCCTCCAGCA GGGCTCGCAGCAGGACGAGGGTGCCCGTCACGCCGCCGATCACCGCGGGGTACGTTTCGTGCGGGCGTTCGTCGAGC GGCAGGAGGGACAGCACCCCGGCCAGGGACGGGCCCGCGGCGCACACCGCCCGCAGCGACGCGGCCAGCTCGGCCGG ATCGGCCCGCCCGTCCGCCACCAGCCGCCGCACCTCGCCGCCGCCCGCCGTGAGGGCCTGCTCACAGGCGTCGGCCC ATGCCCCGGCGCCTTCCGGCCCCACCACCAGCAGCCACGAACCCGCCACGGCCGCGGGGCCGGGGCCGGGCAGGGGG CGCCAGCCGATCCGGTAGCGCCAGGAGTTCACGGTCGTCCGCTCGTGGCGTTCCCGGTGCCAGCGCGACAGCGTCGG CAGCACCGCACCGAGCGACGAGCGCCGCCCGTCCTCCTCACCGAGGCCCAGCGTCTCGGACAGAGCGGGGAGATCCA GCCCGTCGACCGCGCCCCAGAACCGGACCTCCTCCTCGTCCGCCTCCGGCGGCGGCGCGTCCGTCAGCTCGTCCGGC AGCCAGAAACGCTCGCGTTGGAAGGGGTAGGTGGGGAGGTCGGTGGTGGTGGGTTTGGTGGCCGGGTACCAGGGTCG CCAGTTGACGGTGAGGCCGGTGGTGTGGGCGTGGGCGAGGGCGTCGGCGAGGTCGTGGGGGGTGCCGTGGTCGCGGC GGAGGGTGGGGATGGTGGTGGCGGGGATGTCGGCGTCGTCGATGGTGTCCTGGATGCCGGGGGTGAGGACGGGGTGG GGGCCGGCTTCGATGAAGTGGCGGTGTCCGCGGTCCAGGAGGGTGGTGAGGGTGTTGGTGAAGCGGACGGGTTGGCG GAGGTTGGTGAACCAGTAGTCCGCGTCCAGTGGGGTGGTGGTGAGGCGCTCGGTGGTGACGGTGGAGTAGAAGGGGA TGGTGGGTGTGGTGGGGGTGAGGTCGGCGAGTTGGTCGAGGATGGTGTCGCGGATGCGGTCGACGTGGGGGCTGTGG GAGGCGTAGTCGACGTCGATGGTGCGGGTTCGGGCGCCCTGGGTCTCGGCGGCGGTCAGGACGGTGGCGATCTGGTC GGGGGGTCCGGAGATGACGGTGGAGCGGGGGCCGTTGTGGGCGGCGATCGCGACGCCCTCGGCAGTCTCACCCAGGT CGGCCAGGATGCGTGCGGTGTCGTCGGCGGTGAGGGTGAGGGAGGCCATGGCGCCGTGTCCGGCGAGGGCGCGGAGG GCCTGGCTGCGCAGGGCCACGACTTTGGCGGCGTCCTCAAGCGTAAGGGCTCCGGCCACGCAGGCGGCGGCGATCTC ACCCTGGGAGTGGCCGATGACGGCGGCCGGGGTGACACCGAAGTCCTGCCACATGCCGGCCAGGGAGACCATCATGG CCCAGAGGGTGGGCTGGATGACATCGACGCGCTGGGGGTCGGTGGTGGTGTCGGTGCCGCGCAGCACGGCGGTGAGT GACCAGTCGACGTAGGGCGCGAGGGCTTGTTCGCAGGCGGCGATCCTGCTGGCGAAGGCGGGGGAGGAGTCCAGGAG TCCGACGGCCATGCCGGCCCATTGGGAGCCCTGGCCGGGAAAGACGAACACCGGGCCCTGTTGGGGGTCGAGGGCGG TGCCGCGGGTGAGGTGCGGGTGTTCGCCACCGTCGGCCAGGGCGTCGAGCGCGGCGAGCAGTTCGGCGGGATCGGTG CCGGTGACGACGGCACGGCGATCGAAGGTCGCGCGCGTGGTGGCCA GGGACCAGGCCACCTCGGTGGGCGAGACGCCGGGGTTGTCGGTGATGGTCCGGGTCAGCCGGCGCGCCTGCTCGGCC AGGGCCTCGTGGGTACGGGCCGAGAGCACCCAGGGCGCGACACCGCCCAGCACGCCCGGCTCGTCAGCGTCGGGTTC CGGTGCCTCAGGGGCCTCTTCGAGGATGACGTGGGCGTTGGTGCCGGAGATGCCGAACGCCGAGACGCCCGCGCCAC GGGGGCGTTCGGCGGTCGGCCAGTCGACGGGCACGGTGAGCAGACGTACGGCGCCGCTGCTCCAGTCCACGTGTGAG GTGGGCTCGTCGACGTGGAGGGTGGCGGGCAGGGTGCCGTGGCGGAGGGCCATGACCATCTTGATGACGCCCGCGAT CCCGGCGGCGGCCTGGGTGTGGCCGATGTTGGACTTGATGGAGCCCAGCCACAGCGGGCGGTCCTCCGGCCGTTCCT GTCCGTAGGTGGCCAGCAGTGCCTCGGCCTCGATCGGGTCGCCGAGGGTGGTGCCCGTGCCGTGCGCCTCGACCGCG TCCACCTGGTCGGCGGGGAGCCGGGCATCGGCCAGCGCCTGCCGGATGACGCGTTCCTGCGCGGCATCGTTGGGCGC GGCCAGCCCGTTGCTGGCGCCGTCCTGGTTGACGGCCGAGCCCCGGATGACGGCCAGCACGTGGTGTCCGTTGCGGC GGGCGTCGGAGAGCCGTTCCAGCAGGACCAGGCCCGCGCCTTCGGAGAAGCCCGTGCCGTCGGCCGCCGCGGCGAAC GCCTTGCAGCGGGCGTCCGGCGCGAACCCCCGCTGCTTGGAGAACTCCACAAACGCCGTGGCCGCGGTGAACACCGT CACGCCGCCGGCCAGCGCCAGGGTGCACTCGCCCTGGCGCAGCGCCCGGACCGCCAGATGCGTGGCCACGAGAGACG ACGAGCACGCCGTGTCCAGCGTGACCGCCGGACCCTCAAGACCCAGCGCGTACGCCACTCGGCCGGACACCACGCTC GTGAGCCCTCCCGTGGCGGTGTAGCCCTCGAAGCCCTCGGGAATCCGCGGTATCCGCGACAGGTAGTCCTGGCTCGA CACCCCCGCGTAGACGCCGGTGGGCGTGCCCTTGAGGGTGGTGGGGTCGATGCGGGCACGTTCGAGGAGTTCCCAGG CGACCTCCAGGAGGAGCCGCTGCTGGGGGTCCATGGCGAGGGCTTCACGAGGGCTGATGTTGAAGAAGGAGGCGTCG AATTCGTCGGCGTCGTGGAGGAAGCCGCCCTCCTGGACATAGCTGGTGCCGGGGTGGTCCGGGTCGGGGTGGAAGAG GTGGTCGAGGTCCCAGCCGCGGTTGGCGGGAAAGGGGCCGAGGGCGTCGGCGCCCGAGGCTACGACGTCCCACAGGC CCTCCGGAGAGGTGACCCCGCCCGGGTAACGGCAGGCCATGGCGATGATGGCGATGGGTTCCCGGTGGCGTTCTTCC ACTTCGCGCAGGCGCTGTCTGGCCTGGCCGAGGTCGAGGGTGACGCGCTTGAGGTACTGGCGCAGCTTGTCTTCGGT GGTGGACATGATTCCTCCGTCACTCCCCGTCAGGACGAGCCCAGTTGCTGGTCGATGAGCGCGAACATCTCGTCGTC CGACGCCGACTCCAACGCCTCGCCGTCGACGGTGTGATCGACCGCCTCGGCACCGTCACCCAGCCGCCACAGCAGGT TCTGCAACCGCTTGACGAGCTGGGCACCGGTCTCGCCCTCCGGCCGCACCTCGCCCACCGCCGCCTCGAGCCGCTCC AGCTCCCGCAGTACGGGCGCCGCCGCGCCGTCCCCCGCCGGGCACAGCTCGCCCAGCAGGTGCTCGGCCATGGCGGA CGGCGTCGGGTGGCGGAACACCGACGCCGCGGACAGCCGCAGCCCCGTCACCATGGTGAGCCGGTTGCGCAGCTCGA CCGCCGTCAGCGAGTCGAACCCGAGATCCTTGAAGGGCGTGTCCATCGACACCGCGTCGTGGTCCGTGTGCCCCAGC ACGGTGGCCGCGTGGACCCTGACGAGGTCCAGCAGGAGCGCCCGCTGCTCGGCGGGGCCCATCGACGCCAGCCGGCC GGACAGATCGGCCACCGGCGCCACCGCACTCCGTCGGACGGCCCGGGCGCGGGTGCCCGCGAGCGAGCGGACCAGCG GCGGCAGCGCCTCGGCGGGCTGGGCGGACAGCGCGGCCGCGTCGAGCCGGGCGGCGAGCAGCTGCGCCGCACCGTGT CCGCGCGCCGCGTCGAACAGCGCCAGGGCATGCTCCGAGGACAACGGCACCATCCCGGAACGGCTCATCCTGGCGTG GTCCGCCTCGCTGAGCCCGCCGGTCATCCCACTGGTGGCGGCCCACAGGCCCCAGGCGATGGACACGCCGGTGAGAC CCGCATGTTGCCGCCGGGTGGCGAGGGCGTCGCAGTAGGCGTTGGCGGCGGCGTAGTTGGCCTGCCCGGGGGAGCCC AGGGTGCCCGCGGCGGAGGAGAAGACGACGAACATGGCGAGGGGGAGGTGTTCGGTGGCGCGGTGGAGGTTGGTGCC GCTCGTGGCCTTGGCGGCCCAGACGCGGCGGAGGGTGTCGGGGGTCTGGGAGGTGAGGACGGCGTCCTCGACGATGC CCGCGGTGTGGATGACGCCGGTGAGGGGGTGGGCGGGGTCGATGGTGTCGACGATCTGCCGCAGGGCGTGGGGGTCG GTGGTGTCGGTGGCGGTGATGTGGACGGTGGCGCCGAGTTCGGTGAGTTTGTCGGTGAGTTGTTGGGCGCCGGGTGC TTGGGGTCCGCGTCGGCTGAGGAGGTGGAGGTGGGTGATGTGGTGGGTGGTGACGAGGTGTTCGGCGATGAGGGCGC CGAGGGTGCCGGTGCCGCCGGTGATCAGGACCGTGCCGTTGGGGTCGAGGGTGCGGGGGATGTCCAGGACGAGTTTG CCGGTGTGTTTGGCCTGGCTCATGTGGCGCAGCGCTTGGCGGGCCCTGGCGAGTGGCCATGCCTGCACCGGGGGTGG GGTCAGGGTCCCCTGGGTGAAGAGGCCGGCGAGCGTACGCAGCATCTCCGCGACGCGGTCGAAACCGGCGTCGGAGA CGAGGTCGTAGGCGCGGTAGCGGACGCCGGGGTGCGTCGCGGCGATCTCATCCGGTTCGCGGATGTCGGTCTTGCCC ATCTCCAGGAACCGGCCGCCGTCGCCCAGCAGGCGCAGAGACGCGTCGGTGAACTCGCCGGAGAGGCTGTTGAGGAC CACCTCGACCTTCGGGAAGGCGTGCTCGAAGTCCAGGTCGCGGGAGGAGGCGCGGTGGGCTGCGTCGATGCCCATGG CCTCCAGGACGTGGTGTTTGGCGGGGCTCGCGGTGGTGTAGACCTCGGCGCCCAGGTGGCGGGCGATCTGCACGGCC GCCGTACCCACGCCGCCGGTCGCCGCGTGGATGAGGACCGATTCGCCCGGTGTGAGTCCAGCGAGGTCCACCAGCCC GTACCAGGCGGTGAGGTACGCGATGGGTACAGCGGCGGCCTGCTGAT CGGTCCAGCCGGACGGGATCGGCACCAGGGCGCGGGCGTCGGCGACGGCGACGGGGCCGAAGGCGCCCTCGAACAGG CCCATGACACGGTCGCCGACGGCCACCGACGTCACATCCGGAGCGACCTCCAGCACCTCGCCCGCGCCCTCGCCGCC CAGGCCCCGCCGGTCGGGCACGACGCCGAGGGTCACCATCACATCGCGGAAATTGACGCCCGCGGCGCGCACGGCGA TCCGCACCTGACCCGGCGCCAACGGCTCCAGTACGTGCGCGCTCTCCTCGGGCACCAGGCTGTCCACCGTGCCCGCG CTGTCCTCGGCCACGACCAGTTGCCAGGCGGGCGCGCCGACGGGCAGGGCGATGCCGGCCGTCGCGTCCACGGCCGT CAGCCTCGGTACGAATGACCTCCCGTCGCGCACCGCCACCTGCCATTCGTCGGCGTCGAGGGCCGCGCGCGCCACTC CCGCGATGTCGGGTGCCGTGCCGGCGTCGCGTCCGGCGAGGTCGAGCAGCGTGAACCGGCCGGGGTTCTCCGACTGG GCGCTGCGGATCAGACCCCAGACGGCCGCCGCGACCGGGTCGACATCCGTCTCCTCGTCCACGGCGGCGGCGTCGCG GGTGACGATCACCAGCCGGGCCCCGTCGAGCCGCGGCTCGGCCAGCCATTCCTGCGCCGTCGCCAGGACCCGCGCCA CCACCGCGTCGCCGTCCCCGCCGTCGGACTCCGGTGAGCTGGTCAGGACGACCGGTGGCACGGGTGCGCCGTCGTCA AGGGCCGCGGCGAGCGCGGCGACATCCGGGTAGCGGGTGAGCGAGGGCACGGCCTCGGTGGGTCCGCCGTCGCCGAG CACCGCCCAGTCGCCCGCCGCGGCCCTCTCCGGTCCCGCCGGGGTGGGCAGGGCGGTCCAACGCACCTCGTACAGAC CGTCGTTCGGGTCCTCGCTCCGCAGCCGCGCGGTGTCGAGCGGCCGGGTGGTGATGGTGTCGGCGGTGAGGACGAGC GCGCCCGTGGTGTCGGTGAGGGTGAGCCGGTACTCGTTGCCGCCTCGGGGAGTGATGCGGGCCCGCGCGTGGGTGGC GCCGGTCGCGTGGAGGGTGATGCCGTTCCAGGTGAAGGGGAGCCAGATCTCACCGGCCGGGTTGTCGGCGGTGGCGA TGAGGGGGTGGAGCGCGGCGTCGAGGAGTGCGGGGTGGACGGTGAGGGTGTCGGGGGTGTGATCGGCGGTGTCGGAG GTGGGGAGGGCGATGTCGGCGAGGAGTTCGTCGCCGTGTCGCCAGGCGGCTCGCAGCCCTTGGTAGGTGGTTCCGTA GCGATAGCCGCGCGCGGCGGCCCGCTCGTAGAAGCCGTCGATGTCGACGGGTTCGGCTCCCGGGGGCGGCCAGGCCG CCCCCGCTTCGGCGGCCGGCGGCTCGGCCGTGGGCTCGCCGGTGAGGGTGGCGGTGACGTGGTGGGTCCACTCCGCC GTGGGGTCGGACTCCGGGCGGGTGTAGACGTGGACGGTGCGGCGGTCGGTGTCGTCCGGCGGGTTGACGATGACCTG GAGCGCGAGCGGGCGGGCGGGGTCGAGCCTGAGGGGCTGCTGGAGGAGGAGCTCGTCGATGTGGGGTGTGGCGGTGT GGTCCGCGGCGTGGAGGGTGAGTTCGAGGAGCGCGGTGGCGGGCAGCAGGACGGTCTCGGCGATGGTGTGCTCGGTG GGCCATCCGGCGTGGTCGGCGGGAGGGACGTGGCCGGTGACCACCATGCCGCCGTCGGCCAGCGGCGCGGCGGTCCG CAGCAGCGGGTGGTCCAGGCTCGTGGAACCGGCCGCGGCCGGGCCGGAGGCCCCGCCGGTGAGCCAGTAGGGCCGGT GCTGGAAGGGGTAGGTGGGCAGGTCGACGGTGTCCGGGGCGGGCCGGCCGGTGAACCAGGGGCGCCAGTCGACTGGG GTGTGGGTGTGCAGCCGGGCCAGGGCGTGCGCCAGACCCTGGACGTCGGGCTGGTTACGGGTCAGCGCCGGGACGAG CAGAGGAGCGTGCGCGGGCGCGTCATCGAGCGCGTCGAGGGTGGCCTGGGCGGCGGGGATCAGTACGGCGTCCGGGC CGACCTCGAGGAACACCGCCGCGCTCGGCGCGATGTGTGCGACGGCGGGGTGGAAGCGTACGGGCCTGCGGATGTGC CGTACCCAGTAGTCCGGGGTGGCGATGTCCTCGCCCGCGGGTTCGCCGGTGAGGTTGCTGATCAGCGGGGTGTTCGG CTGGGCGAACTCCAGGTCGGCGATGGCGCGGCGGAAGTCTTCGAGAGCGGGTTCCATGAGCGGGGAGTGGAACGCGT GGCTGACGGTCAGGGCCTTGGTCCTGCGGCCGCGTTCCTTCCAGGCCGCGCCCGCCTCGGCCACCAGTTCCGCGGGG CCCGAGATGACGGTGCTGGTGGGGGCGTTGAGGGCGGCGATGGTGACCCGGCCGTCGTACGGGGCGAGGTGCTCGGT GATCTCGTCGGCGGTGGCCTCGACGGCGGTCATGGCGCCGCCGGGCGGGAGGTTGCCGAGGAGGGTGGCACGGGCGG CGACCAGACGGCAGGCGTCTTCCAGGGAGAGGACTCCGGCCACCTGCGCGGCGGTGATTTCGCCGATGGAGTGGCCG ATCACGGTGTCCGGCCGTACGCCGAAGGTCTCCAGCAGCCGTGCCAGGGCCACTTGGACCGCGAAGAGTCCGGCCTG GGTGTAGGTGGTGTGGTCGAGCAGGGTGGCGTCGGGCCGGCCGTGGAGGACGACGTCCCGCAGGGGGTGGTCCAGGT GGGGGTCGAGCAGCGCGCAGACCTCGTCGAACGCGTCGGCGAAGACCGGGAAGCGCGCGTGCAGTTCGGCGCCCATG CCGGGGCGCTGACTGCCCTGCCCGGAGAACAGCCACACGGTCTGCCCCGCCGCGGGCGCGGCGTAGGGCGGGGTGAT CAGGTCCGGGTGGGGACGGCCCTGGGCCAGGGCGTCGAGAGCGGCGGCCATCTCCCGGCGGTCGCCGCCGACGATCA CGGCCCGCCGCTCCAACACCGCGCGCGCCGTGGCCAGCGACCGGCCCACGTCCGCCACCGGCGTGGTGTCGTCGGCT CCCAGCCGGTCGGCGAGGGAGGCGGCCAGGGCGCGCAGGGCCTGGTCGCCGCGGGCGGACAGCGGCCAGGGCACGGG GCCGGGCGGGGGAGCGTCGGTCGCGGGCCGCGGCTCGGGCAGGTCCGGGCCCTGTTCGAGGATGAGGTGGGCGTTGG TGCCGGAGGCGCCGAAGGAGGAGACGGCGGCGCGGCGCGGGCGGCCGGTCTCGGGCCAGTCGGCCGCTTCGGTGAGG AGCCTGACGTTCCCGTCGGCCCAGTCGACGTGCGGGCTGGGCTCGTCGATGTGCAGGGTGGCGGGCAGCGTACGGTG GCGCAGGGCCATCACCATCTTGATGACACCGGCGACACCGGCGGCGCCCTGCGTGTGCCCGATGTTCGACTTGATCG AGCCGAGCCGGAGCGGGTGGCCGCCGGGGCGGTCCTGGCCGTA GGTGGCCAGCAGGGCCTGCGCCTCGATCGGGTCGCCCAGCGTCGTGCCCGTGCCATGGGCCTCGACGGCGTCCACGT CCGCCGGGCCGAGTCCGGCGGTGGTGAGGGCCTGGCGGATCACCCGCTGCTGTGACGGGCCGTTGGGCGCGGTCAGC CCGTTGCTCGCGCCGTCCTGGTTGACCGCCGAACCGCGGACGACGGCCAGAATCCGGTGTCCGTTGCGCCGGGCGTC CGACAGACGCTCCAGGAGGACCAGGCCGACGCCGTCGCCGAAGCCGGTGCCGTCGGCGGCGGCCGCGAACGGCTTGC ACCGTCCGTCGGGGGCCAGACCGCGCTGCCGGGAGAAGCCCGCGAACACGTCAGGGGTGGCCATGACCGTGACGCCG CCCGCGAGCGCCAGGTCGCACTCGCCGTGGCGGAGGGCCTGGGCGGCGAGGTGGATGGAGACCAGGGACGACGAGCA GGCGGTGTCCACGGTGACGGCGGGGCCCTGGAGGCCCAGGGTGTAGGCCACGCGTCCGGCGATGATGCTGCTGGTGC CGGAGAGACCGGCGTAGCCGTCCAGGCGGGGGTCGCCCGGGGGCAGTCCGGCTCCGTAGTCGTGGTACATGACGCCC GCGTAGACGCCGGTCAGGCTGTCCCGGAGGGTGTGCGGGTCGATGCCGGCGCGCTCCAGCGTCTCCCAGGCGGTCTC CAGAATGAGACGCTGCTGGGGGTCCATGGCCAGGGCCTCGCGGGGGTTGATGCCGAAGAAGGCGGCGTCGAATCCGT CGGCGTCGTAGATGAACGCGCCCTGCGAGGCGTAGCTGGTGCCGGGGTGGTCGGGGTCGGGGTGGAACAGATTGGCC AGGTCCCAGCCGCGGTTGGTGGGGAACGGGCCGAGCACATGCCGCCCGGACGCCACCAGGTCCCACAGCCCCTCGGG CGAGGTCACGTCGCCGGGGAAGCGGCACGCCATCGCCACCACCGCGACCGGCTCGTCGGCGGCGCCGGTCCCGGACC GGCCGGCACGGCGCGGCGCGGGGGAGCCGGTGGCGGTCAGGCGCTCCAGGACGTGGTCGGCCAGGGCCCGGGGGGTG GGGTAGTCGAAGACGAGGGTGGCGGGCAGGCGCAGCCCGGTGGCGGCGGCCAGGCGGTTGCGCAGCTCGACGGCGGT CAGGGAGTCGAAGCCCAGCTCCTTGAAGGCGGCCTCGGCCCGGATCGACGCGTCGGAGGAGTGGCCCAGCACCACCG AGACCAGCTGCCGCACCGTCTGGAGCAGCAGTTCGCGCCGTGCGGGCTCGTCGAGACCGGCCAGCCGGCCGGCCAGT GCGGGGCCGGTGGCGCGGTGTTCCATCGCGCGGCGCCGGGCCGGCCTGGCCAGGGCGCGCAGCATGGCGGGGCTGTC GGGGCCGAGCCTGGTGGCGTCGAGGTCGGCGGCCACCACCAGGGGGTGGGTGACATGGAGTGCCGCGTCGAGCAGCC CCAGGCCACGAGCCGTGGCGAGGGGGCGCATGCCGAGGGCGCCGAGGCGGGACAGGTCGGCCTCGGTGAGGTGCTCG GTCATGGCGGAGGTCTGCTGCCACAGGGTCCAGGCGACCGAGGTGCCGGGCAGTCCGTCGGCGCGCCGACGCGCGAT CAGGGCGTCGGTCCAGGCGTTGGCCGCCGCGTATCCGGCCTGGCCGGAGTTGCCGACCGTGCCGGCGGCGGAGGAGA ACACCACGAACATCCGCAGCGGCAACTCCCGGGTGAGGGTGTGCAGATGCCATGCGCCGACGGCCTTGGGCGCCCAT ACGCGGCCGAGTCGGCCGGTGTCCCAGGACTCCACCAGGGCGTCCTCGGTCACCCCGGCGGAGTGAACCACGCCCAG CAGCGGGTGGCTGTCGGGGATCGCCGCCAGCGCCCCGGCGAGCGCGTCGCGGTCGGCCACGTCGACCGCCGCCACCT CCGCGTGGGCGCCCAGCGCGGCGAGCCGCTCGACCAGTTCGGCCGCTTCGGGGCTCTCGGGGCCGCTGCGGGAAAGG AGCAGCACCCGCTCCACGCCATGCGCGCGCACCAGATGTTCGGCGACCGCGCCGCCCAGCACCCCCGTACCGCCCGA CACCAGCACCGTGCCACCGGACAGCTCCGGGGCTCCGGCCCCTGACGTCGGAGAGGCGCGGACCAGACGTGGCGCCA GCACCCCGGCCGCGCGCACGGCGACCTGCGGCTCGTCCGCGTCGAAGGAGTGGAGTACGGCCCCGACGAGGCCCGCT TCACCGTCCGCGTCGCCATCGCCCACGTCGACGAGGGTGAACCGGCCCGGGTGCTCGGCCTGGACCGAACGGACCAG ACCCCACACGGCCGCGCCACCCGGGTCCACCGCCCCCTCGTCCCTGCCCGCGGTCACGGCACCGGAAGTGACCAGGA CCAGCCGGGAGTCCAGCAACCGCGGCTCGGCCAGCCACTCCTGGAGCAGAGCCAGCACGCCCCCGACGGCGGCGAGG CCGTCGTCGGGGGTGGCCGTCGCTGTCACCGGGTGGAGGACAACCGTCGGCACCGGGGTCCCGTCGTCCACCGCGGC GAGCAGCGCGCCGAGGCCGGCGTGTTCCGCCACCCCGGCGAGCGGTGCGCCACCATTGCCCACCGCCGCCCAATCGG CGTCCGGCGGGGCCGAATCGTCGCCGGTCCCGCCCCGCGCGGGCACCCAGTCCACGACGAACAGGCCCTCGGCCGCG CGCTTCCCAAGGGCCCGCAGCTCGGCGACGCCGGTGGGGCGCGCGGCGACCGACGCGGCGTCGAGGACCGGAACGCC CGTCTCGTCGGTCAGGGAGATCCGCCGCCCGTTGCCCTCCGGGGAGATACGGACCCGTACGCGGCGCGCCCCCGTGG CCCACAGCGACACACCGCTCCAGGCGAACGGCAGCCACACCTGTCCGGTGTCCGAGGCGTCCTGACGGCCGCCGTCG AGGAGCGTCGGGTGCAGGGCGGCGTCCAGCAGCGCCGGGTGGACGCCGAACCGGTCGGCGCCCTCACCGGCCGCCTC GGGCAGCGCGACCTCGGCGAGCAGATCGGCGCCGTCCCGCCACGCCGCGGTCAGGCCGCGGAACGCGGGCCCGTAGC CGTAGCCGCCGGCCACGGCCTGTTCGTAGAAGTCCGCGACGTCCAGGGGTTCCGCGCCGGGCGGCGGCCAGGGAGCC GCCACGGCGTCCGACGGGGCCTCACCGCCCTCGGGCGCGAGGACGCCCATGGCGTGGCACACCCACCCGTCGGGGGC CTCCGTGTCGAGCTCGTCGGGGCGGGAGTGCACATCCACCGTCCGCCTGCCCTCGTCGTCGGCGGGCCCCACCGCGA CACGTACGCGCAGCGCCCCCGAGGCGGGCAGGACGAACGGGACCTGGAGGGCCAGCTCCTCCACCGCGGCGCAGCCC GCCTCGTGGGCGGCCTGAAGCGCCCACTCGACCATGGCCGCCCCGGGCAGCAGCGCGGTGTCGCCGACGATGTGGTC GGCGAGCCAGCCCCCGCCCGCGGCGGCCGAGAGGCGTCCGG TCAGCACCATGCCGCCGTCGGGCAGGCTCACCGCGGCGGCCAGCAGCGGATGGTCGACGGCGCGCAGCCCGGCGGCG GACACATCCCCTGTGCCCTTGCCGTCGGCGGGTATCCAGTACCGCCGCCCCTGGAAGGGGTAGGTGGGCAGCTCGAC GGCGCGGGGCGCGGGCCCGGCCGGGAACCACGGGCGCCAGTCGACCTCGAGACCCGCGGTGTGGGCATCGGCGAGCG CGAGGGCGAGCTGGTGTGCGCCGCCGTGGTCGCGGCGCAGCGTGGCGAGAGAGGCGGCGGGGGTGCCGGACTCCTCG AAGATGTCCTGGAGGCTCGTGGACAGGATGGGGTGCGGGTTGCACTGGATGAAGGCCCGGTATCCGTCGCCCAGCAG ACGGCCCACCGCGTCGGCGAAACGGACCGGCCGGCGGAGGTTCTCGAACCAGTACTCGGCGTCCAACCGCGCGGTGT TCAGCCGCTCCGCGGTGACGGTGGAGTAGAACGCGATCTCGGACTCGGCCGGTGTCACCTCGCGCAGGGCGGACACC ACATCCTCGTGGATGCGGTCCACATGAGGGCCGTGGGAGGCGTATTCGACGTCGATGGCGCGAGTGCGCGCGCCACG CTCCCGCGCCGTGTCCAGGACGGCGGCCACCCGGTCCGCGGACCCGGAGACCACCGTGGCGTGCGGGCCGTTGACGG CGGCGACGGCCACGTCCCCGGCGTCGGGGCCCAGCTCGGCGAGGAGCCCGGCGGTGTCCTCGGCGCCGAGGGAGACC GAGGCCATGGCGCCGTGTCCGATCAGGGGACGGAGAGCCTGTACGCGCAGGGCGATGACCTTGGCGGCGTCGTCGAG GGAGAGGGCCCCGGCCACGCACGCCGCGGCCACCTCGCCCTGGGAGTGCCCGACGACGGCCGCCGGGACGATCCCGA AGCTCCGCCACAGGGCGGCCAGCGAGACGTGGATGGCCCACAGCACCGGCTGCACCACATCGTCCCGGCCGGCGTCC GTCGCCCGGTCCGTGCCGCGCAGCACGGCGGTCAGCGACCAGTCCACATACGGCGCCAGAGCCCGCTCGCACTCGGC GATCCGGGCGGCGAAGACCGGCGAGGAGTCCAGGAGTTCGCGCGCCATGCCCGGCCACTGGGCGCCCTGGCCGGGAA ACACGAACACCGGGCCCGCGCCGACCGGGCCCGCCGTACCGGTGACGACCGCCGGATCCGGCTCGTCGGCGGCCAGC TTCGCCAGCCCGCCCAGCAGCGCCGCACGGTCCGCGCCCACCACCACGGCCCGGTGCTCGAACCGGGCCCGCGCCGT CGCCAGAGACCACCCCACCTCCGCCGCACCGGCGGCCGGATCGCGCTCCGCCGCCTCGGCCAGACGCCGCGCCTGTT GCGCCAGCGCCGCCTCGTCCCGCGCGGACAGCAACCACGGCACCACGCCGCCGTTCACCACGGCCCGCGGCGCCACC TCCGCCTCTCCCGTGCCTTCCGGGTCGCTTTCGCCCTGGACCGCCTCGGCGGGTTCGGGCGGCTGCTCGATGATCAG GTGGGCGTTGGTCCCGGAGACGCCGAACGACGACACCCCGGCCCGGCGCGGGTGGTCGGTCTCCGGCCAGTCCACCG GCTCGGTCACCAGACGGATGGCGCCCGATCCCCAGTCCACGTGCGGCGTCGGCTCGTCGATGTGCAACGTCGCGGGG ATCACCCCGTGCCGCATCGCCATCACCATCTTGATCACGCCCGCCGTCCCGGCGGCCGCCTGGGTGTGACCGAGGTT CGACTTGACCGAGCCCAGCAGCAGCGGCCGGTCCGCCGGGCGCTGGTGGCCGTACGTGGCGTGCAGCGCGTCGGCCT CGATCGGGTCGCCGAGCCGGGTGCCCGTGCCATGCGCCTCCACCGCGTCCACCTCGGCGGACGACAGGCGGGCGTTG ACGAGCGCCTGACGGATGACCCGCTGCTGGGAGGGCCCGTTGGGCGCGGTGATGCCATTGCTCGCGCCGTCCTGGTT GACGGCCGAACCCCGGATGACGGCCAGCAGCTGGTGTCCGTTTCGGCGGGCGTCGGAGAGCCGTTCGAGCAGGACCA GCCCGACGCCCTCGCCCCAGCCCGTGCCGTCGGCGGCGGCCGCGAACGGCTTGCAACGCCCGTTCGGCGCCAGGCCG CGCTGACGGGCGAAGCCGGTGAACACCGCGGGCGAGGCCATCAGCGTCACACCACCGGCCACCGCCAGCCGGCACTC GCCCCGCCGCAGTGCGTGCATCCCCAGATGCATGGCCACCAGCGACGCCGAGCAGGCCGTCTCCACCGTCAGCGCGG GACCCTCCAGGCCGAAGGCGTACGACAAACGGCCCGACAGCATGCTGGGCGCGACGCCCGCGTACCCCTCGGTCTCC TTGCGGACGGGAACGCCCGACGTGGCGGAACCGATGGTCGCCGCGCCCACGAAGACACCGGTCTGGCTGCCCCGAAG GCTCGTCGCGTCGAGACCGGCGCGCTCGAACAGCTCCCAGGCCACTTCGAGCAGCTTGCGCTGCTGCGGGTCCATGG CCAGTGCCTCGCGCGGGCTGATCTCGAAGAAGCCCGCGTCGAACGTGTCGGCGTCGCGCAGGAACCCGCCCTCGCGC GTGTACGTCGTGCCGGGGTGGTCGGGGTCCGGGTGATACAGACCGTCCAGGTCCCAGCCGCGGTCCGTGGGGAACGG CCCGACGGCGTCCACCCCCGAGGCCACCAGGTCCCACAACGCCTCGGGCGAATCCACCCCGCCGGCGAACCGGCACG TCATCGCGGTGATGGCGATGGGCTCCTGGTGCCGCTCCTCCACCTCGCGCAGGCGCCGCCGCGTGTCGTGCAGGTCC GTCGCCACACGCTTGAGGTAGTCGACAAGCCTGTCTTCATCCGGCATGTGAAGTCACGACCTTCATACGTGTCGGGG CGGGCAACTGAGCTGTCACGAGGTGCCGAGCTCGTTGTCGATGAACGCGAGCACCTCGTCGGCGGTGGCGAGCGTGA GCCGGTCGGCCGCGCTGTCCTCGGCCGGCGGCGCGGACGCCGCCTTCCATTGGGCCAGCAGACCCTCAAGCCGATCT GTCACCGCCCGCGCGTCCGCCTCGGGCAGCTCGAGCGCCGACAGCGTGGACTCCAGCTTCGCCAGCTCGCCCAGCAC GGGGTCCACCCCGCCCCGGGCCGCCGACCCCCCGTCCGGCACGAGCAGGCCGCGCAGGTGGTCGGCCAGCGCCCTGG CCGAGGGGAAGTCGAAGACCAGCGCGGCCGGCAGCCGGAGCCCGGTCGCCGCCGAGAGCCGGTTCCGCAGCTCGACG CCGGTCAGCGAGTCGAAGCCCAGATCCGTGAACGGCGCGTCCTCCCGTACGCCTTCGGCGTCGGCGTGGCCGAGCAC GGTCGCGGCATGGCCGCGCACCAGCGTGAGCAGCTGCTGCCGCTGCTCGGCCTCCGGGAGCCCGGCGAGCCGGCGCG TCCAGTCGTCGGGGCGCTGGGTGGCCGCGGCGGCGCGCCGCGTGGCCCCGCTCG GGCGGGCGGTCAGGGCCCGTAGCGCGGGCGGCAGACCGGCGATCGGCTGCGCGGCCAGCGCGCGTACGTCGAGGTTG AGCGCCACGAGCTGCGGGTCGCCGTGTCGGTGTGCCGCGTCGAAGAGCGCCAGCGCGTGGTCGGTGGCCAGCGCCGT GACGCCGGTGCGGCCGATCCGCGCCACATCGGCCTCGCTGAGCTGCCCGGTGATATCGCTGGTGGCGGCCCACAGGC CCCAGGCGATGGAGGCGGCGGGCAGACCGGCGTGCTGCCGCCGGGAGGCGAGGGCGTCGCAGTAGGCGTTGGCGGCG GCGTAGTTGGCCTGCCCCGGGTTGCCGAGGGTGCCCGAGACGGATGAGAAGAGGGCGAACATGGCGAGGGGGAGGTG CTCGGTGGCCCGGTGGAGGTTGGCGGCGCCGGTCGCCTTGGCGGCCCAGACACGGTGGAGCGTGTCGGGGGTCTGGG AGGTGATCATCGCGTCGTCCACGGTGCCGGCGGTGTGGATGACGCCGGTGAGGGGGTGGGCGGGGTCGATGGTGTCG ATGATCTGCCGCAGGGCGGGGAGGTCCGAGGTGTCGGTGGCGGTGATGTGGACGGTGGCGCCGAGGGCGGTGAGTTT GTCGGTGAGTTGTTGGGCGCCGGGGGCATCAGGGCCGCGTCGGCTGAGGAGGTGGAGGTGGGTGATGTGGTGGGTGG TGACGAGGTGTTCGGCGATGAGGGCGCCGAGGGTGCCGGTGCCGCCGGTGATCAGGACGGTGCCGTTGGGGTCGAGG GCCGGCGGGATGGTCAGGACGACTTTGCCGGTGTGCTTGGCCTGGCTCATGTGGCGCAGCGCCTGACGCGCCTTGGG CAGCGGCCACGTCTGGACCGGGGGCGGGGTCAGGGCCCCCGCGTCGAACAGGGCCACCAGGGTCGCCAGCATCCGCG CGACGCGCTCCGGCCCGGCGTCCGAGACCAGGTCGTAGACGCGGTAGTCCACCCCGGCGAAGTCCTTGGCCACCTGC TCGGGGTCGCGGAGGTCGGTCTTGCCCATCTCCAGGAAGCGGCCGCCGTCGGCCAGCAGGCGCAGGGAGGCGTCGAT GAAGTCACCGGCGAGGCTGTTGAGGACCACGTCGACCTTCGGGAAGGCGTTCTCGAAGTCGAGGTCGCGGGAGGAGG CGCGGTGGGTCTCGTCGATGCCCATGGCCTCCAGGACATGGTGTTTGCCGGGGCTGGCCGTGGCGTACACCTCGGCG CCCAGGTGGCGGGCGATCTGCACGGCGGCCATGCCCACGCCGCCCGCCGCCGCGTGGACGAGGACCGACTCGCCCGC CCTCAGGCGGCCGAGCTCCACCAGGCCGTACCAGGCGGTCAGAAACGCCACGGGGACGGCCGCCGCCCGGGAGAAGT CCCAGGCCTCCGGCATCCGGGTCACCATCCGCGCGTCGGCCACGGTGACCGGGGCGAACGCGCCGTCGAAGACCCCC AGGACGCGATCGCCCGGGACCACCGTGGTGACGTCCGGGGCCACTTCGAGGACGACGCCCGCGCCCTCGCCGCCGAT GCGGGTCTGGTCGGGCACCATGCCGAGGCTGATCAGGACATCGCGGAAGTTGACACCGGCCGCCCGCACCTCGATGC GCACACGTCCGGGCGCCAGCTCCTCCTCCGCCTCGGGGAAGGGCACCGGCGCCACGGAGTCGAGCGTCGCGGTGCCC GTGGTGCCTGCGGCGTCCAGCCGCCAGGCCCCCTGGCCGACGGGGGGTACGAGCGTGGCCGAGGCGGTGGCGCGGAC GAGCCGGGGCACCAGAATCCGGCCGCCGCGGAGCGCCACCTGCGGTTCGTCGAGGTCGACGGCGCAGCGGACCGCGT CGGCCACCTGGCTGTCCGACTCCGGATCCGTGTCTGTGTCCGTGTCCAGGAGGACGAAGCGGCCGGGGTTCTCCGTC TGGGCGCTGCGGACCAGACCCCAGACGGCCGCCGCGACGGGGTCGACGTCCGTCTCCTCGTCCACGGCGACGGCGTT ACGGGTCACCATCACCAGACGGGCGTCGTCGGGGCGCGGCTCGGCCAGCCACTCCTGTAGCTGCGTCAACGCCGCCT GTATGGGCTCCAGTTCACCGTCGCCCTCCGCGACCGGTGCCTCGGCGAAGACCGCCCGCGAGGCCGGCGTGGCCTCG TCGGCCAGGGGCGAGGCGGGCGTCCACTCCAGGGTGAACAGGCCTTCGGACGAGGGCTTTTGCGGAGCGTCGAGGGC CATGGGCCGGGTGGTGACGGCCTCGGCGGTGAGGATGAGTCGGCCGGTGGCGTCGGTGAGGGTCAGGCTGTGCTCGC TGTCGCGGGAGGTGATGCGGACGCGGGCGTGGGTGGCGCCGGTGGCGTGGAGGGTGACGCCGTTCCAGGAGAACGGC AGCCAGACCTTGCCGTCCGAGGTGTCCGTGACGGCGACGAGGGGATGGAGCGCGCTGTCGAGGAAGGCGGGGTGGAC GGCGAGGGCTTCGGCGGGGTGGCCCGTGGCGTCCGTGGCGTTTGCGGTGGGCAGGGCGATCTCGGCGAGGAGGTCGT CGCCATGGCGCCACAGCGTCTGAAGCCCCTGGAAGGCGGGTCCGTAGTCGTAGCCGTCCGCCGCGGCGTGGTCGTAG AAGCCGGTGATGTCGACGGGCTCGGCGCCCGGGGGCGGCCAGGCCGTCTCAGCTTCGGGCGCCGCCCGGTCGACCGC GGGTTCGCCGGAGAGCGTGGCGGTGGCGTGCCGGGTCCACTCGGCCGCCGGGCCGGACTGCGGGCGGGTGTAGACGG CGAGGCTCCGGCGGTCGTCGTCGTCGGCGCCGACGACGACCTGGAGGTCGAGCGGGCCCGTGGGGTGGAGGACGAGC GGGTGCTGGAGGAGAAGCTCCTCGACGTGCGGGCTGTCGGTGTGCTCGGCCGCGCTGAGGGCGAGTTCGAGGAGTGC GGTGCCGGGCAGCAGCACGGTCCCGGCGATGGTGTGTTCGGTGAGCCAGCCGTCGTGGTCCGCCGCCGGGATCCGGC CGCCCAGAACTAGTCCGCCGTCCGCCAGCGGCGCCGCCGCGTCCAGCAGCGGATGGTCCAGGCCGGTGGCGGCGACG GAGGAGATGCGGGGAGCGATCCAGTACGGCCTGCGCTGGAAGCGGTAGGTGGGCAGCTCGACCACTCGGGGGGCCGG GTCGGTGGGATACCAGGGCGTCCAGTCCAGCGGGGCCCGGGTGGTGTGCAGGGACGCCAGGCCGTGGGCCAGCGCCC GGACGTCGGACTGCTTGCCGGTGAGCGTGGCGAGCGCCGTGGCGGTGGTCTCACCGGGGAGGGTGTGGTGGACGGCG TGGGTCAGCACCGGGGCCGGGCCCAGCTCCAGGAAGACGGCCGTGTCCGCGGCGAGATGGGTGATGGTCTGGTGGAA GCGGACGGGGCGGCGGATGTGCCGGGCCCAGTAGTCGGGGGTGGCGATGTCCTCGTCCGCGGGTTCGCCGGTCAGGT TGCTGATGAGGGGGATACGGGGCAGGCGGAAGTCCAGGTGGC CGATGGCCTCGGCGAACGGGGTGAGGATGGGCTCCATCTGGGGGGAGTGGAAGGCGTGGGAGACCGGGAGGGAGCGG GTCCTGCGGCCCCGGTCCCGCCAGACGGCGGTGACGGTGGCGACCTGGTCGGCGGGTCCGGAGATCACCGTGTGGCC GGGGGTGTTCATCGCCGCGATGGCCAGCTCCGGGAAGTCGGCCAGCGTGGCGGCGACCTCCTCCTCGTCGGCGTTGA CGGTGGCCATGGCGCCGCCGGCCGGCAGGTTGCCCATGAGCGTGGCGCGGGTGGCGACGAGGTGGGCGGCGTCCTCC AGGGAGAGGACGCCCGCGAGGTGGGCGGCGGAGATCTCGCCGATGGAGTGTCCGGCGACGGCGTCGGGGGCCAGGCC CATGGCCGTCAGAAGGCGGGCGAGGGCGATGTGGAGGGCGAACAGCCCGGCCTGGGTGTAGGTGGTGTGGTGGATCA GGTCGCCGAGTTCGGGGTCGGTGGCGAAGACCACGTCGCGCAGCGGGCGGTCGAGGTGGGGGTCCAGGAGCGCGCAC ACCTCGTCGAAGGCCCCGGCGAAGACGGGGAATCGCCGGTGGAGCTCGGCGCCCATGCCGGGGCGCTGGCTGCCCTG CCCGGAGAACAGCCACACCGTCTTGCCCGGGGTGTCCGGGGCTGCGCCGCCGGGGGTGGCGAGGCCGGGGTGGGGCT CCCCGCCGGCCAGCGCCTCCAGGGCGGCGGTCAGCTCCTCGCGGCCGTCCCCGACGATCACGGCCCGCCGCTCGAAC ACCGCCCGTGTCGTGGCCAGGGACCAGCCCACCTCGCCGACCGGGAGATCGGGGCGCGCGGCGAGGTGTTCGGCGAG CGCCGCGGCCTGTTCGCGCAGGGCCCGGTCGTCGCGGGCCGACAGCGGCCAGGGCACGGGGCCGGGCAGGGGAGTGT CGCTCGCCGGCCGGGGTTCGGGGAGGTCGGGGGCCTGTTCGAGGATGAGGTGGGCGTTGGTGCCGGAGGCGCCGAAG GAGGAGACGGCGGCCCGGCGCGGGCGGCCGGTGTCGGGCCAGCCGACGGGCTCGGTGAGGAGCCGGACGTTTCCGTC GGCCCAGTCGATGTGCGGGCTGGGCTCGTCGATGTGCAGGCTGGCGGGCAGTTGGCCGTGGCGCAGGGCGGAGATCA TCTTGATGACTCCGGCGATGCCGGAGGCCGCCTGGGTGTGGCCGATGTTGGACTTGACCGAGCCGAGCCACAGCGGC CGGTCGTCGGGGCGGTCCTGGCCGTAGGTGGCCAGGATGGCCTGTGCCTCGATGGGGTCGCCCAGCGTGGTGCCCGT GCCGTGGGCCTCGACGGCGTCGATGTCGGCGGTGGTCAGGCCGGCTCCGGCGAGGGCCTGGGCGATGACGCGCTGCT GGGAGGGGCCGTTGGGGGCGGTCAGCCCGTTCGACGCGCCGTCCTGGTTGATCGCCGAGCCGCGCACCACCGCGAGC ACCCGGTGGCCGTTGCGGCGCGCGTCGGAGAGGCGTTCCAGCAGGACGAGCCCGGCCCCCTCGCTCAGGCTGGTGCC GTCGGCCGCGGCGGCGAACGGCTTGCACCGTCCGTCGGGCGCCAGGCCGCGCTGCCGGGAGAAGCCGGTGAACGGAT CGGGGGTGGCCATGACGGTCACGCCGCCCGCGAGCGCCAGATCGCACTCGCCCTGCCGCAGCGCCTGGGCGGCCAGG TGCATGGACACCAGGGACGACGAGCACGCGGTGTCCACGGTGACGGCGGGGCCCTGAAGCCCCAGGGTGTAGGCCAC GCGGCCGGAGATGATGCTGCCGATGGTGGAGAGCATCGTGTAGCCGTCGAGACGCGGGTCGCCCGCGGGCAGGCCGG TGGCGTAGTCGTGGTAGATGACGCCCGTGTAGACGCCGGTCAGGCTGTCCTTGAGGGTGTGCGGGTCGATCCCGGCG CGCTCCAGGGCCTCCCAGGCGGTCTCCAGGACGAGCCGCTGCTGCGGGTCCATCGCCAGGGCCTCACGGGGGTTGAT GCCGAAGAAGGCCGCGTCGAAGCCCTCGACGTCGTAGATGAACGCGCCCTCGGAGGCGTAGCTGGTGCCGGGGTGGT CGGAGTCGGGGTGGAACAGATTGGCCAGATCCCAGCCGCGGTTGGTGGGGAACGGCCCGACCACATGCTCCCCCGCC GAGACCAGGTCCCACAGCCCCTCGGGCGAGGTCACGCCACCAGGGAAGCGGCACGCCATCGCCACCACCGCGATGGG GTCGTCGTCCGCGGCGCGAGCCGACCGGCCCCGCCCGATGAGGGGCGAGGCGGTGGCGGACGCGGTGGCGGTCAGCC GCTCCAGGAGATGGCCGGCGAGGGTCTGGGGGGTGGGGTAGTCGAAGACGAGGGTGGAGGGCAGGCGCAGCCCGGTG GCATCGGACAGCCGGTTGCGCAGCCCGACGGCCATGAGGGAGTCGAAGCCCAGCTCCTTGAAGGCGGCCTCGGGCCT GATGGACGTGTCGGAGGAGTGGCCCAGGACCACGGCGGCCATCTGACGCACCATCTCCAGCAGCAGATCGCGCTGTC CCGAGGCGTCGAGACCGGCGAGGCGTGCGGCCAGGGCGGGGGACGCCGACTGGCGTTCCGCGGCGCGGCGCCGGCCG GACCGAACGGGCGCGCGCAGCAGCGCGGGGACCACGGTGGCCGAGATGGCCGCGGCGGTCGACCGGGCGGTGTCGAG GTCGGCGGCCACGACCGCGGGGTGTGGGAGGGAGAACGCCGCGTCGAGCAGGCGCGGGGCGTGGGACGCGGCCCGCG CCTCCAGGGCCCAGGCCACGCTCAGGCCGGGGAGCCCGGCGGCTCGCCGATGGGAGATCAGGGCGTCGGCGCGGGCG TTGGCGGCGGCGTGACCGGCCCGGCCGGGGCCGCCTACGAGGTCGGCGGCCGAGGAGACCACCACGAACAGCCGCAG CGGCAGATCGCGGGTGAGGGTGTGCAGATGCCACGCCTCGGTGGCGGGCGCCCAGGCCCGCGTCAGCCGCTCGTCGT CCCAGGACTCCACCGGCGGCGCGCCGTCGGCCGCCCCGGCGAGATGCACCACGCCCACCAGCGGACGGCTTTCGGGT ACGGAGGCCAGAACCTCCGCCAGCGCGGAGCGGTCGGCCACATCGACGGCGGCGGTCTTCGCCTCCGCCCCCAGCGC CGTCAACCGCTCGGCCGGCGCGGCCGCTTCGGGATCCTGGCCGCCCCCGGGGGAGAGGAGCAGCACACGCGCCACGC CGTACGCCCGCACCAGGTGTTCGGCGACCGCGCCGCCCAGCGGGTCCGTCCCGCCCGACACCACCACCGTGCCACCG GACAGATCGACCTCGGCGGCCGCGTCGGTCGGTTCGACGGCGCGCCGCAGACGGGGGATCGACACCGCGCCCGAACG GACCGCGATCTGCGGCTCGTCGGCGTCGAGCGACCGGCGGACCGCCTCGGCCCACTTCCCGTCCCCGTCCGCGTCGG CATCGACCAGGGTGAACCGGCCCGGGTGTTCGTTCTGGACT GCCCGGACCAGGCCCCAGACGGCCGCGCCTGCCGGGTCCACCGGCCCCTCGCCGTCGCCGACCGCTACCGCGCCGGA GGTGATCAGCACCAGCCGGGAGTCCCGCAACTGAGGTTCCGCAAGCCAGCCCTGGACAAGGCCCAGCGTGTGCCGGA CCGCGGACAGGTCCGCGTCGGGGTCGGGATTCGGGGCCACGGTGGCGAGGACGACGGGCGGCGCGGGCTCTCCGCCC TCCACCGCGGCCAGCAGCGCGGCCAGGTCGGCGTACCGGCGCCCGCCGAAGGGGCGCGCCGCGGCAGGAAGGGCGTC GAGCGTGGCCCAGCCGGCCTCGGCCTCCTCCGGCTCGGGACGGTCCTCCGACGGCGGCAGCGGCGTCCACTCCACCG TGAACAGGCCGCCCGCGGCGTGTCGTTCGGCGGCTTTCAGCCAGTCGCCGTCGGCGGGACGCAGCGCGACCGTATCC ACGTCCAGCACCGGGGCGCCGGTCGCGTCCGCGACGACGACGCCCAACTCGCGGCCCCCGCTGTCCTCCCGCCCCAG CGACCGGATCCGGACCCGGACACTCCGTGCGCCGCCCGCGTGCAGGGACACACCGCGCCAGGCGAACGGCAGCCACC GCGGGCCGTCGGAGTGAAGGTCGTGCAGCGCCACCGGGTGCAGGGCGGCTTCGAGCAGCGCGGGATGGATGCCGAAC TCCTCGCCCCTCTCCTCGGCCCCGTCGGGCAGGGTCACCTCGGCGAAGAGATCGGAGCCGTGCCGCCACACCGCCTG GACGCCCTGGAACGCGGGGCCGAACCGATGACCTTCGGCCTCGGCGCGCGCGTACAGCTCGGCGATGTCGGCGGTGT CCACGGGCTCGGCCCCGGCCGGGAGCCAGGCGCCGGTCAGCTCCGTCCCGTTCGTTGTTTCGCGGGCGAGGGTGCCC GTCGCATGGCAGGTCCAGGCGTCCCGCCCCTCGGGCCCCTCCGGTGTGCCGGACGCGGGCTGGGAACAGACGCGTAT ACCGCGCCGCCCATCCCCGTCGGGCGCGTCAACCACGACCTGGACGCGCAGTTCAAAGGCGGGGGAGAGGACCAGGG GGACCTGGAGGGTCAGCTCCTCGACCGCGCCGCAGCCCGCCTCGTCGGCCGCCCGCAGGGCCCACTCCACGAGCGCC GTGCCGGGCAGCAGGACGGTGTCGCCGACCGTCTGCTCACCGAGCCGGCCCGCGTCACCGGCCGCCGGAAGCCGCCC GGTGAGCAGCAGCCCGCCGTCGGCGAGGCCCGTCGCGGCCGACAGCTGAGGGTGTGCCACCGGCCGCAGTCCGGCGG CGCTGACGTCGCCTGCCGGGCGGCCGGCCGCCATCCAGTGGCGCTGCCGCTGGAAGGGGTAGGTGGGCAGGTCGGCT GTGGTGGACGGGGTGACGGGATACCAGGGTCGCCAGTTGACGGCCAGGCCGGTGGTGTGGGCGTGGGCGAGGGCGTC GGCGAGGTCGTGGGGGGTGCCGTGGTCGCGGCGGAGGGTGGGGATGGTGGTGGCGGGGATGTCGGCGTCGTCGATGG TGTCCTGGATGCCGGGGGTGAGGACGGGGTGCGGGCTCACTTCCACGAAGTGGCGGTGGCGGTGGGCCAGGAGGGCG GTGAGGGTGTCGGCGAAGCGGACGGGGTGGCGGAGGTTGGTGAACCAGTACTCGGCGTCCAGCGGGGTGTCCGCCAG TGGCTCGGTGGTGACGGTGGAGTAGAAGGGGATGGTGGGTGTGGCGGGGGCGAGGCCGGAGAGCCGGTCGAGGATGG TGTCGCGGATGCGGTCGACGTGGGGGCTGTGGGAGGCGTAGTCGACGTCGATGGTGCGGGTTCGCGCGCCTTGGGTC TCGGCGGCGGTCAGGACGGTGGCGATCTGGTCGGGGGGTCCGGAGATGACGGTGGAGCGGGGGCCGTTGTGGGCGGC GATGGCGATGCCTTCGGCAGTCTCGCCCAGGTCGGCCAGGATGTGTGCGGTGTGGTCGGCGGTGAGGGTGAGGGAGG CCATGGCGCCGTGTCCGGCGAGGGCGCGGAGGGCCTGGCTGCGCAGGGCCACGACTTTGGCGGCGTCCTCAAGCGTA AGGGCTCCGGCCACGCAGGCGGCGGCGATCTCACCCTGGGAGTGGCCGATGACGGCGGCCGGAGTGACACCGAAGTC CTGCCACATACCGGCCAGAGACACCATCATGGCCCAGAGGGTCGGCTGGACGACATCGACGCGCTGGGGGTCGGTGG TGTCCGTGCCGCGCAGCACGGCGGTGAGTGACCAGTCGACGTACGGGGCCAAGGCCTTTTCACAGGCGGCGATCCTG CTGGCGAAGGCGGGGGAGGAGTCCAGGAGTCCGACGGCCATGCCGGCCCATTGGGAGCCCTGACCAGGGAAGACGAA CACCGGGCCGTCCTGGATGTCCAGGGCGGCCCCGCGCACGAGGTGGGGGTGTCCGCCGCCGGTGGCCAGGGCCTCCA GCGCGGCGGTGAGCCCCGGCAGGTCGGTTCCGGTGACCACGGCGCGGTGGTCGAAGGTGGCGCGGGTGGTGGCCAGC GACCAGGCCACCTCGGTCGGTGAGATACCCGGAGTGTCCGCGATGGCCCCCGCCAGTCGGCGCGCCTGCTCGGCCAG GGCCTCGTGGGTACGGGCCGAGAGCACCCAAGGCAACACGCCGCCCACGACGACCGGTTCGGCCGCATCCGTGTCGG GTTCCGGTGCCTCAGGGGCCTCTTCCAGGATGACGTGGGCATTGGTGCCGGAGATGCCGAATGCCGAGACGCCTGCC CGGCGCGGGCGTTCCGCGGTCGGCCAGTCGACCGGCTCGGTGAGCAGGCGCACCGCGCCGCTGCTCCAGTCCACATG CGAGGTGGGCTCGTCGACGTGGAGGGTGGCGGGCAGGGTGCCGTGGCGCAGTGCCATGACCATCTTGATGACACCCG CGATCCCGGCGGCCCCCTGGGTGTGGCCGATGTTCGACTTGATCGAGCCCAGCCACAGCGGGCGGTCCTCCGGCCGC TCCTGGCCATAGGTGGCCAGCAGTGCCTCCGCCTCGATCGGGTCGCCGAGCGTGGTGCCCGTGCCGTGCGCCTCCAC CGCGTCCACCTCGACCGCGGCCAGCCCGGCGTTGGCCAGGGCCTGGCGGATCACCCGCTGCTGCGAGGGCCCGTTGG GGGCGCTCAGCCCGTTGCTGGCGCCGTCCTGGTTGACGGCCGAGCCCCGGATGACGGCGAGCACGTGGTGTCCGTTG CGGCGGGCGTCGGAGAGCCGTTCCAGCAGGACCAGGCCCGCGCCTTCGGAGAAGCCCGTGCCGTCGGCCGCCGTGGC GAACGGCTTGCACCGCCCGTCCGGGGCCAGGCCGCGCTGCCGCGAGAACTCCGTGATCACGTTCGGGGTCGACATGA CCGTCACACCGCCGGCCAGCGCCAGATCGCACTCGCCCTGCCGTAGCGCCTGGACCGCCAGATGCATCGCCACCAGC GACGACGAGCACGCCGTGTCCAGCGTGACCGCCGGACCCTCCAGG CCCAGGGTGAAGGCGATCCGGCCGGACACCACGCTGAGCGCGTTGCCCGTCAGCAGAAAGCCCTCCGCGCTCTTCTC GATGTGCGGGGTGCCGAAGCCGGGCACGCCGCAGCCCGTGTAGACGCCGGTGGAAGAGCCCTTGAGGGCGGTGGGGT CGATATGGGCGCGTTCGAGGAGTTCCCAGGCGGTTTCCAGGAGGAGCCGCTGCTGGGGGTCCATGGCGAGGGCTTCA CGCGGGCTGATGTTGAAGAAGGAGGCGTCGAATTCATCCGCGTCGTGGACGAAGCCGCCCTCCTGGATGTAGCTGGT GCCCGGGTGGTCGGGGTCGGGGTGGTAGAGGGCGGCGAGGTCCCAGCCGCGGTTGGTGGGAAAGGGGCCGATGGCGT CGGCGCGGGTGTCGACCAGGTCCCACAGGGCCTCCGGGGATGCGATGCCACCGGGGTAGCGGCAGGCCATGGAGGTG ATGGCGATGGGCTCGTGGTGGCGTTGCTCCGCTTCGCGCAGGCGTTGTCTGGCCTCGCCCAGGTCGAGGGTGACCCG CTTGAGGTACTGGCGCAGCTTGTCTTCCGTGGACGGCATTGGTCCTCCCCCACTCCTCGTCAGGACGAGCCCAGTTG CTGGTCGATGAGCGCGAACATCTCGTCGTCCGAGGCCGACTCCAGCGCCTCGCCGTCGATGTCCTTGGCCCCGGCCG TGCCGCCCTCCAGCAGGGACAGCAGAGCGTGGAGCCGGCCCGCGACCGTCTCGCGGGCCTTCCCGTCCATGGCGTGG CCGGTCAAGGTGGACTCGAGCCTTGCCAAGTCGCCCAGCACGGAACCGAGACGGTCCACCGCCGCGCCGGGCGTCCG GTCCGCGGGGGCGAGCTTCTGGTCCAGATAGCGGGCCATGGTCTCCGCGTCGGGATAGTCGAAGACGAGGGCGGCGG GCAGCCGCAGCCCGGTCGCGGCCGAGAGCCGGTTGCGCAGCTCGACGGCGGTCAGCGAGTCGAACCCCAGCTCCTTG AACGAGGTGTCCGCCCGCACGGCAGCCGGGTCGCTGTGGCCCAGCACCGTCGCCGCGTGCGTACGCACCAGGTTCAC CAGCTCGCGGTGGCGCTCCGCGTCGGAGAGCGCCGCCAGACGGCCCGCCCAGTCTGTGGACCGGGCGGCGGCCGCGG CGCGCCGTACGGGCGCTCCGGCCGCCGCGATCGTACGGAGCAGGGCGGGCACGGCGTCGGACGGCTGGGCGCCGAGC GTACGCGGATCGAGGTCGAGCGCCAGCGGAGCCGCGCCGCCGTGTCGCAGGGCGGCGTCGAACAGCGCCAGCGCCAT CGGGGTGGACAGCGGGGTGAAGCCGGTGCGGCTCATCCGCGCGAGGTCGGCCTCGGTGAGCCGGCCCGTCATATCGC TGGTGGCGGCCCACAGGCCCCAGGCGATGGACACGCCGGTGAGACCGGCGTGGCGCCGCCGGGTGGCGAGGGCGTCG CAGTAGGCGTTGGCGGCGGCGTAGTTGGCCTGCCCGGCGGTGCCGAGGGTGCCTGCGGCGGAGGAGAAGACGACGAA CATGGCGAGGGGGAGGTGCTCGGTGGCCCGGTGCAGATGAGCCGCGGACGTCGCCTTCGCCGCCCAGACCGGCGCCA GCCGCTCCGCGGTCTGCGCGGTCAGCACCGCGTCGTCCAGAGCCCCCGCGGTGTGGATGACGCCGGTGAGGGGGTGG GCGGGGTCGATGGTGTCGATGATCTGCCGCAGGGCGGGGAGGTCCGAGGTGTCGGTGGCGGTGATGTGGACGGTGGC GCCGAGGGCGGTGAGTTTGTCGGTGAGTTGTTGGGCGCCGGGGGCATCAGGGCCGCGTCGGCTGAGGAGGTGGAGGT GGGTGATGTGGTGGGTGGTGACGAGGTGTTCGGCGATGAGGGCGCCGAGGGTGCCGGTGCCGCCGGTGATCAGGACG GTGCCGTTGGGGTCGAGGGTGCGGGGGATGTCCAGGACGAGTTTGCCGGTGTGTTTGGCCTGGCTCATATGGCGCAG CGCCTGGCGCGCCCTGGTCAGGGGCCATGCCTGCACCGGGGGCGGTTGGAGGACGCCCTGGGTGAAGAGGTCGGCCA GCGTGCCCAGCATCTCCGCGACCCGGTCCAGACCGGCGTCCGGAACCAGGTCGTAGAAGCGGTAGCGGACACCCGGG TACGTTGCGGCGATCTCCTCGGCGTCGCGCTTGTCGGTCTTGCCCATCTCGATGAACCGGCCGCCGTCGCCCAGCAG CCGCAGGGAGGCGTCGGTGAACTCGCCGGCCAGGCTGTTGAGGACCACGTCGACCTTCGGGAAGGTGTCCTCGAAGT CGAGGTCGCGGGAGGAGGCGCGGTGGGCGGCGTCGATGCCCATGGCCTCCAGGACGTGGTGTTTGGCGGGGCCGGCC GTGGCGTAGACCTCGGCGCCCAGGTGGCGGGCGATCTGCACGGCCGCCGTGCCCACGCCGCCGGTGGCGGCGTGGAT GAGGACCGATTCGCCCGGTGTGAGTCCAGCGAGGTCCACCAGCCCGTACCAGGCGGTCAGGAACGTCGTCGGGACGG CCGCGGCCTGCTGGTCCGTCCAGCCGGGCGGGATCGGCACCACGGCGCGCGCGTCGGCGACGGCGACCGGTCCGAAG GCGCCCTCGAACAGCCCGAACACCTGGTCGCCGACGGCCACCGACGTGACGTCCGGCGCCACGTCGAGCACCACGCC CGCGCCCTCGCTGCCGCGGAACACCCCTTCGTCCGGGTACATCCCGAGCACGATCAGCACATCGCGGAAGTTGATGC CCGCGGCGCGCACGCCGATGCGCACCTGACCCGGCGCCAGCGGCTCCAAGACCTCGGGGCAGGCCACGACCGACACG CTGTCGACGGTGCCGGTGCCGTCGGACGCCAACCGCCAGGCCGGTTCGCCCGGCGGCGGCGCCAACTCCGCTGCCGC CGCCCGTTCCAGCCGTGGCGCCAGCACGCCTCCTCCGCGCACCGCCACCTGCGGTTCGTCGAGCGCCACAGCCCGCG TGACGGCGCCCCGTACCTGCTCGGGCCCCGCGTCGTCGTCGATGTCGCACAGCACGAAGCGGCCCGGGTTCTCCGCC TGGGCGCTGCGCACCAGGCCCCACACGGCGGCCGCCGCCGGGTCGGGCCGGGCGGCCCCGGGGCCGTCGTCGACATC CCCGGCCGCCACCGCGCCACGGGTGACGAGCACCAGCCGAGCCTCGGCGAGCTCCGGCCTCTCCAGCCACCGCCGCG CCGACGCCAGCACACCCGTCACCACGGCCGGCGCGGCGGCGGACGGCTCGTCGCCGCCCTCGGCCGCGGGCACGCCG ACGAGCACCGTGTGGGGCGTGTCCCGGCCCGCGTCGTGGGCGGCGAACAGGGCGTCGAGGTCGGCGTAGCGGGGCAG CTGAGGATCTGCCGAGCCGAACGGGAGCGGGCCGCCTTCCAAGACCGCCCACCCCGGCGCCATCGTGTCGGACTCCG CTTCCAGGGGCGGCACAGGCGCCCACGCCACCTCGTACAGCCCGTCCAC CCGCCGCGCGCCCGTCTCGCGCAACTGCCGTGCGCTTGTCGGGCGTACGACCACGCAGTCCGCGCTGAGCACCGTAT CGCCGGCCGCGTCGGCCACCATGAGCCGGAAGCCGTGCTCGAGCGGCGACATACGGACCCGCACCGCGCGGGCGCCG GTGGCCCACAGCGACACGCCGCCCCACGCGAACGGCAGCCGCAGTTGCCCGTCGTCGCCCTGGCCGTCCAGCGCCAT GGGGTGCAGGACCGCGTCCAGCAGCGCGGGGTGGAGGGAGAACCCGTCGGCGCCCTCGTGGGCCGCCTCGGGCAGCA CGACCTCCGCGAGCAGGTCGGCGCCCTGCCGCCAGGCGGCGGTCAGGCCCCGGAAGGCCGGGCCGTAGCCGTAGCCG ATCGCCTCGGCGCGGCCGTAGAAGTCGGACACGTCCACCGGCTCGGCGTCGGGTGGCGGCCACGTCCCCGTAAACGC CGCGTCCGGCACGCCTGGCACACCCGGCGCCGTCGGAGTCGTCGGGGCGAGGTGTCCGGTGGCGTGGCAGGTCCAGG GCGACGCGCCGTCGTCCGCGTCCGGGCGGGAGTGCACCCGCACCTCGCGGCGGCCCTCCTCGCCGGGCGCGTCCACC ACGACCTGGACCCGCAGCCCGCCGGACGCGGGCAGCGCCATCGGGATGTGGAGAGCGAGCTCCTCGATTCCGCCGCA GCCCGCCTCGTCGGCCGCCCGCAGCACCCACTCCACCAGGGCCGCGCCCGGCACCAGGGGAGTGCCGGCGACGACGT GGTCGGCGAGCCAGCCGCCGCCCCCGGCGGCGGGGAGCCGCCCGGTCATCACCAGCCCGCCGTCGGCCAGTTCGACC GCCGCGCCGAGCTGGGCGTGCGCCACCGAGGTGAGCCCGGCGCTCCGCAGATCGCCCGGGCCGCCGTCCATGCCCAG CCAGTAGCACCGCCGTTGGAAGGCGTAGGTGGGCAGGTCGGTGGTGCGCGGTGGCGGGTCGGCGGGGTACCAGACGT CCCAGGCCACGGCGAGCCCGGCGGTGTGCGCGGCGGCGACGGCCTGGGCGAGCCGGCGGGGCCCGCCCTGGTCGCGG CGGAGGGTGGGGATGGTGGCGGCGGAGATGTCCGCCGCCTCGAAGGCGTCCTGCATCCCGGGGGCGAGGACGGGGTG CGGGCTCACCTCGACGAAGTGGCGGTGTCCGCGGTCCAGGAGGGCGGCGAGGGTGTCGGTGAAGCGGACCGGCCTGC GGAGGTTGGTGAACCAGTAGTCGGTGTCGAGCCCGGCGGTGTCGAGACGCTCGGCGGTCACCGTGGAGTAGAAGGCG ATGTCCGACGCGGCGGGCGTCAGCTCGCCCAGGGCGGAGACGATGTCCTCGCGGATGCGGTCGACATGGGGGCTGTG GGAGGCGTAGTCCACATCGATGGTGCGGGTCCGCGCGCCACGGGCCTCGGCGGCCGCCAGGACGGTGGCGATCTGCG CCACCGCGCCCGAGACGACCGTGGTGGCAGGGCCGTTGGCGGCGGCCAGGGTGACATCGTCGGCCTGGTCGCCCAGC TCCTCCAGGAGCCGGGTGGTGTCGTCGGCGCCGAGGGTGAGGGAGGCCATGGTGCCCTGGCCGGCCAGGGCGCGCAG GGCCCGGCTGCGCAGGGCCACGACCTTGGCGGCGTCGTCGAGGGTGAGGGCTCCGGCCACGCAGGCGGCGGCGATCT CGCCCTGGGAGTGGCCGATGACGGCGGCGGGGGTGACCCCGAGGTGTTGCCAGAGGGCGGCGAGGGAGACCATCACG GCCCAGAGGGTGGGCTGGACGACATCGACGCGCTGGGGGTCGGTGTCGGTGTCGGTGTCCGCGCCGCGCAGCACGGC GGTGAGTGACCAGTCCACGTACGGCGCCAGGGCTCGCTCGCACTCCGCGATCCGCGCCGCGAACACCGGTGAGCCGT CCAGCAGCTCCACGCCCATGCCCCGCCACTGCGACCCCTGGCCCGGGAAGACGAACACCGGGCCGGCGGGCGCCGCG ATGGCCGTTCCCGCCACGACCGCCGGGTCGGGCTCGTCGGCGGCCAGCCTCTCCAACCCGGCCAGCAGCGAGTCCCG GTCGTCGCCCACGATCACGGCCCGGTGCTCGAACGACGACCGCGTCGTCGCCAACGACCAGCCCACCTCGGCCGGCT CGGTGTGGGGGAGCCGCGTGGCCAGGTTCGAGGCCTGGGCGCGCAGCGCTTCGGCGGTGCGCGCGGAGAGCACGAGG GGCAGGACGCCGGGCCGGGAGGTGCGCTCCGGCTCGGGCTGTTCGGGGGCTTGTTCGAGGATGAGGTGGGCGTTGGT GCCGGAGATGCCGAAGGAGGAGACGGCGGCGCGGCGCGGTCGGTCGGTGTCGGGCCACTGGGCGGCCTCGGTGAGGA GGCGGACGTTTCCGGTGGCCCAGTCGACGTGCGGGCTGGGCTCGTCCACGTGCAGGGTGGCCGGGAGGTGGCCGTGG CGCAGGGCCATCACCATCTTGATGACAGCGGCGGCACCGGCGGCGGCCTGGGTGTGGCCGATGTTGGACTTGATCGA GCCCAGCCACAGGGGCCGGTCCTCGGGGCGGTTCTGTCCGTAGGTGGCGAGGAGGGCCTGGGCCTCGATCGGGTCGC CCAGGGTCGTGCCCGTGCCGTGGGCCTCGACGGCGTCCACGTCCGTCGGATCCAATCCGGCCGTGGCCAGGGCCTGG CGGATGACGCGCTGCTGCGAGGGGCCGTTGGGCGCGGTCAGGCCGTTGCTCGCGCCGTCCTGGTTGATCGCCGAGCC CCGCAGGATCGCGAGCACCCGGTGCCCGTTGCGCCGGGCGTCCGACAGCCGCTCCAGCACCAGCAGCCCGGCGCCCT CGCTCCAGCCGGTGCCGTCGGCGGCTGCGGCGAACGGCTTGCACCGCCCGTCCGGGGCCAGGCCGCGCTGCCGGGAG AACTCGACGAACGTGTTCGGCGTGGCCATCACCGTCACCCCGCCCGCGAGCGCCAGATCGCACTCACCCTGCCGCAG CGCCTGCGCGGCGAGGTGCATTGCCACCAGCGACGACGAACACGCCGTGTCCACCGTGAGCGTCGGCCCCTCCAGAC CGAAGGTGTAGGCCACCCGGCCGGACGCCACGCTCGGCGTGGAGCCCGTCATGATCTGGCCCTCGTACCCCTCCGGG ATCCGGCCGAGGAACCGCGCGCCGTAGTCGTCGTACATGATGCCGGTGACCACCGCCGTCCGGCTGCCGCGCAGCGC CACCGGATCGATGCGGGCGTGCTCGAACGCCTGCCACGCCGTCTCCAGCAGCAGCCGCTGCTGCGGGTCGGTGGCCA GCGCCTCCCGGGGGCTGATCCCGAAGAACTCCGCGTCGAAAAGGGCGGCTTGGTGCAGAAAGCCGCCCTCCCTGGCG TAGCTCTTGCCCGTCCGCTCGGGGTCGGGGTCGTACAGCCCGGCCACGTCCCAGCCGCGGTCCTCGGGGAACGGCCC GATCGCGTCCACCCCGTCGGCGACCAGCCGCCACAGCCCGTCCGGGGA GTGCACATCGCCCGGGTAGTGGCAGGCCATGCCGACGATCACGACGGGGTCGTCGGCGTTTCCGGCCCGGGTCGGCG CGCCCGCTCCCGGCGGCAGCGGCGCGGCGGCCGCGGTGGCGCCGAGCAGTTCGGTGCGCAGATGGCTCACCAGGGTC TCGGGCGTCGGGTAGTCGAAGACCGCGGTGGCCGGCAGGCGGACGCCCGTCGCGTCGTGGATCGCGTTGCGCAGCTC CAGCGCGGTCAGCGAGTCGAGGCCGAGCTGCTGGAAGGTCCGGCCCAGTTCGAGGGTTTCCGGCTCGGGGAGGGCCA GGACGGCGGCGATCCGGTCGCGTATCAGCTCGCCGATCACCTGGTCCTGCTCGGCCTCCGGCAGCCCGGCCAGCCGC CCGGCCCAGGAGGGGGACACGGCGTCCGGCGCGGCCGTACGCGCCGTACGCACCGGGACGCGCACGAGGCGGCGCAG CAGCGGGGGCAGCGTTCCGGCGGCGGCCTGCTCGCGCAGCACGGCGAGTTCGAACCTGGCCGGGACCAGCACGGGCG GCGCGGTGGCGAGCGCCGCGTCGAACCGGGCCAGGCCCTGCTCCCCGGACATCGTGGCGGTGCCCGAACGCGCCATC CGGGCCAGATCCGCGGTGGCGAGGTGTTCGGTCATCCCCTCGTCCACCCCCCACAGGCCCCAGGCCAGCGAGGTCGC CCGGCCGCCCGCCGCGCGCACGTGTTCGGCGAGGGCGTTGAGGAACGCGTTCGCCGCGCCGTAGTTGCCCTGCCCGC CGCTGCCGAGCACCGAGGCGGCGGAGGAGAACAGCACGAACGAGGCGAGGCCCGCGCCCTCGGTCAGCCGGTGCAGA TGCCAGGCCGCGTCCACCTTCGGCGCCAGGACGGCGTCGAGTTGGCCGGGCGTCAGCGAGGTGACCACGCCGTCGTC GAGCACGCCCGCGGTGTGCACGACCGACGTCAGCGGGTGCTCGGCGGGGACGGTGGCGAGCAGCGCGGCCAGCGCCT CGGGGTCGGAGGTGTCACAGCTCTCGACGCGTACGTCGGCCCCGAGCGCGGCCGCGAACGCGTCGATGCCCTCGGCG GCCGGACCGCTCCGGCTGACCAGCAGCAGATGCCGGACACCGTGGGTTTCGACGAGGTGCCGGGCGAGCAGCCGGCC GAGCGAGCCGGTGCCGCCGGTCACCAGTACGGTGCCCTCGGGGTCGAACGCCGTCGGCGCCGTGTCCTCGTCGGGGA GCGCCGCGACCAGCCGGGGCACCAGCACCTGCCCGTCGCGGAGCGCCAGTTGCGACTCGCCCGCGGCCGTGGCGGCC GCGACCGCCGCCGCCAGCGCGTCCCGGGACGCCGGGCTGTCATCCGTGTCCACCAGCACGATCCGCCCCGGGTGTTC GGCCTGCGCGGCGCGCAGCAGGCCCAGCATCGCGGCGCCCGCCAGGTCGGCCGGAGTGTCGGCCGGGTCGGCGGCGA TGGCTCCCGTAGTGAGCACCACCAGACGGCCGGTGGCGAGGGCGTCGTCCGCCAGCCACTCCCGCAGCCACGCGAGT ACGTGGTGCGTGGCTGCGCGCACCCGGGCGGGCGGCGCGTCGCCATCGGGGGCGCAGAGGCAGCCCATGGCGATCAG GTCGGGGGCCGGGGCCCCGGCGGCGACCGAGGCGCTCAGCGCGGCGAGGTCCGGATGGCGCGCCACGTGCTCATCGG GCAGTGCGGCGGTGGCGCCGTCGGTCCGGTCCGTGCCGTTCGCGTCGACCACGCCCCAGCGGTCCGCCGTCGCCGTC GGCACGGCGGCGGGCAGCGCGAAGGGCTCCCAGTCCAGCCGGTACAGCGAACGGTCGGCCTGCGCCGCCCCGGACCG CCACCGCTCGGCGGAGACGGTGCGCAGCGTGAGGGCGTCGACCGTGGCGACCTCTGCGCCGGCGTCGTCGGACAGCC GCATGGCCACGCTGTCCGGCCCGGTCGGCGTGATGCGGACCCGCAGCCGGGACGCGCCGCGCGCCGACAGCCGCACA CCGCCGAAGGAGAACGGCAGCCGGACCGTGCCCGGCGCGCCGCCCGCCTGGTCCTCGCCGGTGAACAGCGCGCCGAT CGGGTGCAGGGCGGCGTCGAGGAGGGCGGGGTGGACGGCGTACGCCCGGCCGTCGTCCCGCAGGCCGGGGTCCAGGC TCACCTCGGCGAACATCTCCTCGCCGACTCGCCACGCCGCGCGCAGCCCCGTGAACACAGGTCCGTAGCGGTACCCC TCGTCGGCCAGCCGCTCGTACAACCCGGCCACGTCGACCTCTTCCGCCCCGGCCGGTGGCCACGGGGCAGGCGGCGC CGCGGGCTCGGGAGTGGCACTGGTCCCCGCGGCGGTCAGCGTGGCGGTGGCGTGCCGGTGCCAGGGTCCGTACGGCT CATCGGCACCGTCCGACGCCTCGGCGCCGCCGCCGGACGGGCGCGAGTAGACGGTGACGGCCCGTCGGCCGGCCTGG TCGGCTGCCCCGACGGCGACCTGGAGATCCACCGCCCCCACCGGGGGCAGCAGCAGTGGCGCCTCCAGGGTGAGGTC CGCGATCCGGTCGCAGCCGGTCCGTTCGCCCGCCTGGAGCGCGAGTTCCACGAAGAGCGTGCCGGGCAGCGGCACCG CGTCCACGATGGCGTGGTCGGCCAGCCAGGGCTGACCGCGCAGCGACAGCCGGCCGGTCAGCAGCAACCCGCCGTCC GCCGGTTCGAGCGCGGCGCGCAGCAGAGGGTGGTCGCTCGCCTCGAGCCCGGCGGCCCGTACGTCCGCCTCCGCTTG CGGGGCGTCCCGCCAGTAGCGGCGGCGGTCGAAGCGGTAGCCGGGCAGGTCGGTGGCCGGGGTCCCCGGCGGGAAGG CGGCGGCGAAGTCGACGGTCTCGCCGTCCACATGGAGCCGCGCGAGCGCGGTCAGCAGCGTACGGGACTCCTCGCGG CCGCCGCGCAGCGCCGCGGCGAGCACCGGCGCGCCCGCGCCCCCCGATCCTCCGCCGGCACCTTCCGCTGCCAGGCA GTCGCGGGCCATGGTGGTGAGCACGGCGTCCGGGCCGAGTTCCAGGAAGCGGGTGACGCCCTCCGCGCGCAGGGCGG CGATGCCGTCGCGGAACCGCACCGGCTGCCGCGCGTGCCGCACCCAGTAGTCGGGGTCGCACAGCTGCTCGGCGCCG GCCAGCGCGCCGGTCACGTTCGAGACGACCGCGATCCGCGGCGGGGCGTACGTCAGCGAGGCGGCGACCCGCCGGAA CTCCTCCAGCATCGGATCGATCCGCGGCGAGTGGAAGGCGTGGCCGACCCGCAGCCGCCGGGTCCGGTGTCCGCGCT CCCGTATCGCGCGGGCGACCTCCTCGACCGCCTCCTCGTCGCCCGACACCACCACCGACGTCGGCGCGTTGACGGCC GCCAGCGCCACCCGGTCGGCCCGTCCGGCGAGCAGCGGCGCCACCTCTTCCTCGGCGGCCTCCAGCGCGATCATCGC GCCACCTTCCGGCAGCGCCTGCATCAGCCGCCCGCGGGCGGCC ACCAGCCGCGCCGCGTCCGGCAGCGACAGCACCCCGGCGATGTGCGCGGCGACCAGCTCGCCGACCGAGTGCCCCGC CAGATAGCCGGGCACCACGCCGAACGACTCCACCAGCCGGTACAGGGCGACCTCGACCGCGAACAGCGCGGCCTGCG TCACCCCCGTCGCGTCCAGCGGACCGGCCTCGTCCCCGTCCCCGTCGGCGGGCGCGAACACCAACTCCCGCAGCGAA CGCCCCAGGTGGGGGTCCAGCGCCGCACACGCCTCGTCGAACGCGGCGGCGAACACCGGATAGGTCCGGTACAGCCG GGCGCCCATGCCGGGGCGCTGGCTGCCCTGCCCGGTGAAGAGGAACGCGCTGCCACCGAGCCGCGCCGGGGCGGACG CCCGCGTCACATCGGGATGTGTACGCCCCTCGGCGAGCTCGGCGAGCCGGGCACACAGCTCCTCGCGGTCCGTGCCC AGGACGACGGCGCGGTGGTCGAGCGCGGACCGGGTGGCGGCCAGCGACCAGCCCACCGCGTCGACCGGGAGGTCCGG GCGCGCGGCGAGGTGTTCGGCGAGCGCTGCGGCCTGCGTGCCCAGCGGCGCCGCGCCGCGCGCGGAGAGCACCCACG GCACGGCGGCGCCCTCCGGGCGTGGCGGCGTACCCGCGGGCAGCTCCGTGTCCGTGTCCGGCAGGGGCGGGGCCTCT TCGAGAATCAGATGCGCGTTGGTGCCGCTGATGCCGAAGGACGACACACCGGCCCGGCGCCGCCGCTCCCCGGTCGG CCACGCGACCGGCTCGGCCAGCAGCCGCACCGCTCCGCCGTCCCAGTCGACCACGGGCGTGGGCCGGTCGGCGTTCA GGAGGGCGGGCAGCGAGGCGTTCCGCAGCGCCATGACCATCTTGATCACGCCCGCGACACCCGCCGCGCCCTGGGTG TGTCCGATGTTCGGCTTGACCGCGCCCAGCCACAGCGGCCGGTCCGCGGGCCGCTCCTGACCGTACGTGGCCAGCAG CGCCTGGGCCTCGATGGGATCGCCCAGTGTCGTGCCGGTGCCGTGTGCCTCGACCGCGTCCACGTCCGCCGGAGCGA GCCGGGCGCCGGCCAGCGCCTGCCGGATGACGCGCTGCTGCGACAGTCCGTTGGGCGCGGTCAGCCCGTTGCTCGCG CCGTCCTGGTTGATCGCCGAGCCGCGCACCACGGCCAGGACGCGGTGACCGTTGCGCCGGGCGTCCGACAGCCGCTC CAGCACCAGCAGCCCCACGCCCTCGGCGAACGCGGTGCCGTCGGCCCCCTCGGAGAAGGCCTTGCACCGGCCGTCCG CCGCCAGCCCCCGCTGCCGCGCGAACTCCGTGAACACGTAAGGAGTGGTCATGACCGTCACCCCGCCCGCCAGCGCC AGTTCGCACTCGCCCTGACGCAGCGCCTGCGCGGCGAGGTGCATCGCCACCAGCGACGAGGAACACGCCGTGTCCAC CGTGACCGCGGGCCCCTCCAGACCCAGGGTGTACGCCACCCGGCCGGACAGCACACTGGGCGCGTTGCCGGTGACCA GATAGCCCTCCGCGGCCCGGTCGATGTGCGGGGTACCGAAGCCGGGCAGGGCGGTGCCCGCGTACACGCCGACGGGC GCGCCCTTCAGCGACCCGGGCGCGATGCCCGCCCGCTCCAGCGCCTCCCAGGCCACCTCCAGCAGCAGCCGCTGCTG CGGGTCGATCGCGGCGGCCTCCCGGGGGCTGATGCCGAAGAACTCCGGGTCGAACAGGTCGGCGTCGTGCAGGAAGC CGCCCTCGCGGGCGTAGCTGGTGCCCGGGTGGTCGGGGTCAGGGTGGAACAGCCGCTCCAGGTCCCAGCCGCGGTTG TCCGGAAACGCGGTCATCGCGTGCCGGCGGGCGGCGACCAGGTCCCACAGGTCCTCGGGCGAGGCCACCCCGCCGGG GTAGCGGCAGGCCATCGCCACGATGGCCACCGGCTCCCGCTGCCCGTCCTCGACCTCGCGCAGCCGCGCCCGGGTGT CGTGCAGATCGGCGGCGACCCGCTTGAGGTAGTCGACCAGCCGCTCTTCGTTGGTCTCTGCGGTCACGGCATGTCCC TCCCCACCCGCTCAGTCTTGGCGCGCTTCAACGCGCGGGCTTCCTTCGTCACTGCTCGCTCCTTCGTCGCTGCGCGG CTCCTCAGTCCAGCCCGCACAGCGCGCCAAGACACGCTCACTTCGCCTGCCGCGCTCGTCGAGGCTTCTCGGGCTCT CTCGGCGTTCGTGCCAGACCGGGCTCATTGCACTCCGAGTTCCTTGTCGATGAAGTCGAGGACCTGGTCGGTCGTCG CGGCTTCGAGCCGCTCGGCCGCGCTGCCGCCGTTCCGGCCCGGGGCGTGGGCCGCCTTCCAGGCGGCCAGCAGCGAC TCCAGACGCGCCGTCACCGCGCCGGGGTCGGCCCCTCCGGCGCCGTTCACACCGCCGTTCGCGCCGCCGTTCGCACC GGACGCGGTGAGCGCCGTCAGCGTCTCCTCCAGACCCGTCAGCCGGCTGAGCACGGGCGCGTAGAGGTCGGGCGCGG TCGCCGCCGCCTCCTCGGGGGCCAGTCGGCCGCGCAGATGCTCGGCCAGCGCCACCGGCTCCGGGTAGTCGAAGACG ACGGTGGCGGGCAGCCGTACCCCGGTGGCGGCGGTGACGCGGTTGCGCAGCTCGACGGCGGTCAGCGAGTCGAACCC GAGCTCCTTGAACGCCGCGTCCGCCGGTACGGCGTCGGGGTCGGCGTGTCCGAGCACGGTGGCGGCGTGTTTGCGGA TCAGATCGGTGAGCAGGCGGCGCTGTTCGGCCGGGGCGAGACCGCCCAGCCGGGCCGCCCAGCCGCCGGGCTCCTCG CCCGCCGCCGCGGTCGGCCGCGCCGCGCCCCCCGGCCCGCCGGAGCGGGCCCCCGCCGCCGACGCCTCCGCTGCCAG GGCGCGCAGCGGCACGGGCAGGGCCGCGAGGGGCTGCGCGGCCAGCGCCCGGGTGTCCAGGTTCAGCGCGAGCAGCG CCGGCCGGCCGTGCTCCACCGCCGCGTCGAACAGCTCCTGCCCCTGCTCGGCGCTGTTCGGAACGATGCCCTGCCGG GCGATCCGCGCCACGTCCGACTCGGTCAGCCCTCCGGTGAGCCCGCTCGCGGTGGACCACAGGCCCCAGCTCACCGA CACGCCCGGCAGACCCTCGGCGTGGCGCCGCTCGACCAGGGCGTCGCAGAAGGCGTTGGCGGCCGCGTAGTTGGCCT GCCCAGGGGTGCCGAGGGTGGACGCGAACGAGGAGAACAACACGAACATGCCCAGCGGCAGCCCGGCCGTCGCGCGG TGCAGATGGGCCGCCGCCGCGGCCTTGGCCGCCCAGGCCTCGGCCATGCGCTGCTCGTCCTGCGCGGGCAGCATCGC GTCCGCCACCACACCCGCCGCGTGGACGACCCCGGTGAGCGGATGCGCCGGGTCGATCCCCGCGATTGCTTCACCCA GCGCGTCGGCGTCCCCGACGTCCACCGCGGCCACCCGCACTCGTGCGCCGAGTTCGG TGAGCCGGGCCGTCAGCTCCGCCGCGCCCGGCGCGTCGGGGCCGCGCCGGCCGAGCAGCAGCAGATGCCGAACCCCG CCCGAGCGCACCAGGTGTTCGGCGATCAGGCCGCCGAGGGTGCCGGTGCCGCCCGTGATCAGCACCGTGCCGTCCGG GTCCGGCGCGGGCGCCACGTCCAGCACCAGCTTGCCGGTGTGCCGGGCCTGGCTGAGCTGCCGAAGCGCCTGCCTGA CCTGGCCGAGCCGCCACGCGGTGACGGGCAGGGGCGTGAACGCGCCGGTGGCGAACCGCTCGCCCACGGCGGCCAGC AGCCGCCCGATGTGCTCCGGACCGGCGTCCGCGATCAGGTCGAACGCCCGGTACGCCACGGAGTGGTCCCGCGCGAC CCGCTCCGGGTCGCGTACGTCCGTCTTGCCCATCTCGATCAGCCGGCCGCCGGGCGCGAGCAGCCGCAGCGAGGCGT CGGTGAACTCCCCGGCCAGGCTGTTGAGGACGAGGTCGACGCCCCGGCCGCCGGTCGCCGCCGAGAACACCGCCTCG AACTCCAGGTCCCGAGAGGACGCCCGGTGTGCGGCGTCCACGCCCATGCCCTCCAGCACCGGGTGCTTCGCGGGGCT CGCCGTGGCGAACACCTCCGCGCCCAGGTGCCGGGCGACCTGGACCGCGGCCATGCCCACTCCGCCGGTCGCGCCGT GGATCAGCACGGTCTCGCCCGGGCGCAGCCCGCCCAGCTCCACCAGGCCGTACCAGGCGGTGAGGAAGGCCACCGGG GCGGCCGCGGCCTCCCGCAGGCTCCAGCCGTGCGGCACGCGGGCGACCATCCGGGCATCGGCCACCGTCACAGGGCC GAACGCACCGTCGAACAGGCCCATCACACGGTCGCCCACGGCCACGCCGGTCACCCCGTCGGACACCTCCACGACGA CCCCGGCGCCCTCGTTGCCGATCGACGCCTCGGCGTCCGGGTACATGTCCAGGGCGATCAGCGCGTCGCGGAAGTTG AGACCGGCGGCGCGCACGGCGATCCGCACCTCGCCGGGCGCGAGCAGGGCCGAGGCCTGAGGGCTTGAGGCCGTGGT GAGGTCGTCCGGGCCGGTCTTTCCGGCCACCACACGCCAGGCCCGCTCGCCCGGAGGGGCCGCCAGCTCCGGCTCGG TGTCCGCCCGGGCCAGCCGGGGCACCAGCGCCCGCCCGCCCCGCAGCGCGAGCTGCGGCTCGTCGGCGCGTACTGCC TCCCGTACCGCTTCGGCCAGTCCGGTGTCGGCGTCGCAGCCGGTGTCGCAGCCGGTGTCGATGAGGAGGAAACGCCC CGGGTGTTCCGTCTGGGCGCTGCGTACGAGCCCCCACACCGCTGCCGCGGCGGGGTCCACCGGCTCCTCGCCGGCGC CACCGGCCCGCACCCCAGAACGCGTGACCACGGCCAGCCGTGCGCCCTCGGTCACGCTCCCGGCGGCCAGCCACCGC TGAGCGAGCGCCAGTGTCCGGCGCGCGGTGGCCAGACCCGTGTCCGCCCCCGCCGGGTCCGGGCCGGTCCGCACCGG GGCGACGACGACATCCGGGACGGGGGCGCCGCCGTCCACGGCGTGGCACAGCGCGTCGAGATCGGCGTAACGGGCGG CATCGGCCAGGCCCGGGGGGCCCTGGGCGTCATTGCCCACCACCGCAAGTGTCACCGGAACAGCCCCGGCCTCTTCA ACCGCCGCAGCGGGAAGGGGCGTCCACTCCACCGCGAACAGCCCGTGCGGCCCCCGCGCACGGCTCGCCGCGCGCAG CTGACGGCCGTCGGTCTCGCGGAGCGCGACCGCGTCCGCGGTCAGCACCGGGGAGCCCGTGGCGTCGGCCAGCAGCA CCCGCAGCCCGCCCGGCCGCGCCGACAGCCGGACCCTGAGCCGCGTGGCGCCACCCGCCCACAGCCGCACCCCGCTC CAGGCGAACGGGAGCAGCACCGCCGCGCGCCCCGCGTCGGCGCCCGGCGCGGCGGTGTCCAGGTGACCGGTCAGCAG CACCGGCTGCATCGCCGCGTCGAGCAGGGCGGGGTGGATACCGAAGCTGCCCGCCTGGTCCGCCGCCTCCTCGGGCA GCACCACCTCCGCCAGCGTGTCCTCGCCGTCGCGCCATACGCTCCGCAGCCCCCGGAACACCGTCCCGTAGCCGTAA CCCGCCGCCGCGGCCCGCTCGTAGAAACCCTCGATCGCCACCGGGCGCGCCCCCGGCGGCGGCCACGCCCCGCCCGG CTCCGGCGCCGGGAGCGGCCCGTCCGCGCCGGGGAGCAGCGTGCCCACGGCATGGCAGATCCAGGTGTCCGGCCCGC CGGAGTCCTCCTCGGGCCGGGAGAAGACCCGCACCGGACGGCGCCCGTCGACCTCCGCGGCGTCCACGACCACCTGG ACCACCAGCCCGCCCGAGGCGGGAAGCGCCACCGGGACCTGGAGCGCCAGCTCCTCGACGCCGCCGCAGCCCGCCTC GTCGGCCGCCCGCAGCACCCACTCGACCAGCACCGCGCCGGGCAGCAACGGCAGGCCCGCGACAATGTGTTCGGCCG GCCAGCCGCCGCGCGCCGCCTCCGGCAGCCGCCCGCTGAGCAGCAGGCCGCCGTCGGCCAGACCCACGGCCACCGGG AGCAGCGCGTGTCCGATCGCGGTGAGCCCGGCCCCCGCCACATCGCCGGGACCGGTGCCGCCGCCGGTCGGCAGCCA GTACCGCTCCCGCTGGAAGGCGTACGACGGCAGCGGCACCGCCGTGGGCGCGGGCGCCCCGGCGTACCAGCTCGCCC AGTCGACCGCCACGCCCGCGACATGCGCCTCGGCCAGCGCCCGGCACAGCCGCTCCGGGCCGCCCTCGTCGCGGCGG AGCGTGCCGATGGCCGCGGCCGGGGAGCCCGCCGCCTCGATGCACTCCTGAAGGCCGAGCAGCAGCACGGGATGCGC GCTGACCTCGATGAACACCCGGTAGTCGTCCGCCAGCAGCGCCTGGACCGCCTCGGCGAAACGCACCGGCTGCCGCA GGTTGGTGAACCAGTAGCCGGCGTCGAGGCCGGAGGCGTCCATACGGGCACCGGTCACCGTCGAGTAGAAGGCGACA TCGGACGCGGTGTCGGACGCGGCGCCGGACACCCCGGCGAGCACCTCGGCCAACACGCCGGCGATGCCGTCCACCTG AGGGCCGTGCGAGGCGTAGTCCACGTCGATCGCGCGGGCCCGCAGCCCGTCGGCCCGGGCGGCGGCCACCAGCTTCG CGATCTGCTCCGGCGGCCCGGACACCACCGTGGACGACGGGCCGTTGACCGCCGCGACCGCCACGTCCGGCGCGTGC AGGGCGGTCAGCTCCTCGGTGCGCTCGACGCCACAGCCCATCGAGGCCATGGCGCCGTGCCCGGCCAGCGCCCGCAG CGCCCGCGCCCGCAGCGCCACCACCCGGGCGCCGTCCTCCAGCGTCATCGCGCCCGCGACACACGCCGCCGCGATCT CCCCCTGCGAGTGGCCCACCACGGCGGCCGGTACCACGCCGTGCGCCTC CCACACCGCGGCCAGCGACACCATGACCGCCCAGAGCACCGGCTGCACCACATCGACCCGGCCGATGTCGGCCGCGC CCTCCGCGCCGCGCAGCACATCGGTCAGCGACCAGTCGACATGCGCGGCCAGGGCCCGTTCGCACGCGGCGACGCGC GCCGCGAAGACGGGGGAGGAGTCCAGCAGCGCCACGCCCATACCGAGCCACTGCGACCCCTGCCCGGGGAACACCAT GACCGGGCCCGCGCCCACGTCCGCCGCCACGCCCCGCACCACCGCGGGGCCCGGCTCGCCCGCCGCCAGCCCTTCGA GCGCGGCCAGGAGGTCCGGCCGGCCACGGCCCACCACGACGGCCCGGTGCTCGAACGCCGTGCGGCGCGTCGCCAGC GACCACCCCACGTCGACGGCGCCACTCGCCACCCCGTCCGCCGTCAGGAAGGCGCCCAGACGCGCGGCCTGGTCCCT CAGCGCCGCCTCGGTGCGCCCCGAGACGAGCCAGGGCACCACGCCGCTCTCGGCGTTGACCCGCTGCTCATCTGTCT GACCGTCGGCGTCCGGGGCCTGTTCGAGGATCACATGCGCGTTGGTGCCGGAGACGCCGAACGACGACACCGCCGCC CGGCGCGGCCGCTCGGCCACCGGCCAGTCCCGGGGCTCCGTCAGCAGCCGCACCGTGCCACCGCTCCAGTCCACGTG CCGGGTCGGCTCGTCGATGTGCAGGGTCCGGGCCAGCTTGCCGTCCCGCAGCGCCATCACGGTGTTGATCGCGCCCG CGACGCCGGCCGCCGCGTGCGTATGGCCGATGTTCGACTTGATCGAGCCGAGGTGGAGCGGCCGGTCCGCCGGGCGG TCCGCCCCGTACGTGGCGATCAGCGCCTCCGCCTCGATGGGGTCGCCCAGCCGGGTGCCGGTCCCGTGCGCCTCGAC CATGTCCACGTCCGCGCCGGACAGGCCGGCTCGCGCCAACGCGTCCCGGATCACGTTCTCCTGGGCGACGTCGTTGG GCGCGGTGAGCCCGTTGCTCGCGCCGTCCTGGTTGATGGCCGAGCCGCGCACCACGGCCAGGACGCGGTGTCCGTTG CGCCGCGCGTCGGACAGCCGCTCCAGCAGCAGGACGCCGGCGCCCTCGGAGAAGCCGGTGCCGTCGGCGGCGGCCGC GAACGGCTTGCACCGGGCGTCGGGGGTCAGCGCACGCTGCCGGCAGAACTCGGTGAACATCAGCGGCGTGGACAGCA CGGTGACCCCGCCCGCGAGCGCCAGGGAGCACTCGCCCTGCCGCAGGGCCTGCCCCGCCAGATGGATGGCCACCATC GACGACGAGCACGCGGTGTCCACGGTGACCGCGGGTCCCTCGAAACCGAAGGTGTACGCCACGCGGCCGGAGATGAC GCTGGTGAGGCTGCCCGTGGTGGCGTAGCCCTCGAAGCCCTCCGGGATCCGCGGCAGGCCGGAGATGTAGTCCTCGC TCGACACCCCCACGTAGACGCCGGTCCGGCTGGCGCGCAGGGCGCCGGGCACCACCCCGGCGGACTCCAGCGCCTCC CACGACAGTTCGAGGAGCAGCCGCTGCTGCGGGTCCATCGCCAGCGCCTCGCGCGGGCTGATCCCGAAGAACCCCGC GTCGAAACGGTCGACGTCGTGCAGGAAGCCGCCCTGGCTCACATAGCTGGTGCCGAAGTTGGCGGGATCCGGGTGGA AGAGGCCGTCCAGGTCCCAGCCGCGGTCGGTGGGGAAGTCGCCGATCGCGTCGCCGCCCGACAGCACGAGGCGCCGC AGGTCCTCGGGGGACCGCACCCCGCCGGGGAAGCGGCAGGCCATGCCGACCACCGCGACCGGCTCCTGCTCCCCCTC CTCCAGCTCCCGCAGACGGGTCCTGGCGTCGTGCAACTCGGTGGTGACCCGGCGCAGGTACTCGACCAGTTTCTCTT CGCTGACCATGTCGTCACCTGCCTTCAGTGGGAGGGGCGCGGACGTGCGTCGGAGAGCACGCCGAGCTCGTTGTCGA TGAAGTCGAGGATTTCGGCGGCCGTGGCGGACGCCAGCTTGTCCGCGGCGTCGAGCGTGGAGCCGTTGCCGTTGCCG TTGTGCGGAGTGTCCTGTGCGCTGTTCGGGGTGTCGGGAGCGAGCTGCTGCTCGATACGCCGGGCCAGCGCCTCCGG CGTGGGGTGCCGGAAGATGACGGCGGCGGGCAGCCGCAGCCCGCTGGCCGCCACCAGCCGGTCGCGCAGCTCGACGG CGGTCATCGAGTCGAAGCCCAGCTCCTTGAACGACGAGTCGCCGCGCACGGCCTTGGGGTCGCTCTGCCCGAGCACC GCGGCGGCGTGGTCGCGCACCAGCTCCAGGACGGCGCGGCGCCGTTCGGCGGGCCCGAGGCCGGCCAGCCGGCTCGC CAAGTCGGCGGTGCGGCCCGGGGCCGCGGCCACCGGCCGGACCGGTGCCCGGCCACCCGCGCTCGCGGCGAGTGCGC GCAGCGCGGCCGGCAGCTCGTGGCCCGGACGGGCGGCCAGGGCCCGGACCCGGATGTCGGCCACCACCAGCCCGGGG CGGTCGGCGTGGCAGGCGGCCCGGAACAGCGCCGCCCCGCGGTCCGCGCCGAGCACCCCCGCCCAGCGCCCGCCGGA GCCAGCCCCGGAACCCGCCCCGGAGCCGGCATCGGCGCCTATGGCAGCGGCCCACGGCCCGAACGCCACCGCGAGGC CGGGCAGCCCGGCCCGGCGCCGCGCCGCCATCACGGCGTCGCAGAACGCACCGGCCGCGGCCCGTTCGGGCGACACC GGACCGCCCCAGGTCGCGGCGGCGTCCGACAGGGTGACAAACCGGACCGACGGCAGTCCGGCGGTGGCCCGTTGCAA CGCCGTCACCGCCCCGGCCACCGCGGCCCACCCTCGTACCGTGTCACCCGCCGACTCGACGGCGACCGGCTCGATGG CGGACGACAGCCCCGCGACGTGGACGACGCCGGTCAGTGGATGGGCCGGCTCGACCCGCGCCACCACCCGTTCCAGC GCGGCGGCGTTGAGCGGGTCGACCGTGACCACGCGCACCCGCGCGCCCCACCGCTCCTCACCGCTCCCGCCGTCGTC CGTACTCTCCGCGCCGCCGAAGCGGACCAGTAGCAGCCGCCGGACGCCCTCGGCGCGGGCCAGATGCTCGGCGACAG CCTCGGCGAGCGGGCTTCCGGCGTCCGTGACGAGGACCGTGCCCTCCGCGTCGAACGCCCCGTCGCCCGGGGCCGCG GCGGAGCGCGCCAGGCGTGGCGCGAGCAGTGTGTCCCCGCGCAGCGCGACCCTCGGCTCGTCGAGCGCGGCCACCCG CGCCACGGCCGCCGGACCCGCCTTGGCGCCGATGCCGGTGTCGGTGTCGGTGTCGATGAGCAGGAAGCGGCCGGGAT GTTCGGCCTGGACACCGCTCGCGTACCCCCAGGCCGCCGCCGCGGCCGGGTCCACCGGATCGCCCTCGGAGCCGCCG GGCGCCACCGCGCCCCGGGTGAGCACCGCGAGCCGGCTGACCGCCAGCCGC GGCTCGGCACACCAGCCGCGCAGCAGCGCCGCGACGCCCCGTACGGCGGACAGCGCCGCCTCGTCCGGCGCGGCCCC GGGGTGAGCGGCCGGTGTGCGCCACAGCACCAAGGACGGCGCGGGCGCTCCGGCGTCCAGCGCGGCCAGCAGCGCCG GAAGGTCCGCGTACCGATCGGCGTTCGCGTCGGCCGACTCCGTCTCCGACTCCGACTCCGTCAGCGCCTCGCCCAAC ACGGCCACCCGCCCCGTGTCGTCCTCGGCGCCGGAGGGCTCCGCGGGGGCCACCGCGGTCCAGTCGACGGCGAAGAG ACCACCCGCGGCGGCCCCGCTCGCCTCGCGTATCTGCCGTACGCCACGCGGGCGCAGCACCACCGACTCGATGCTCA GCACGGGCGCCCCGGCCGGGTCGGACACCTCAATCCGCAGCTCGCGTTCTTCGTCTGCTTCGTCCCACGGCGACAGC CGGACCCGCGCGGCGCGCGCCCCGGTCGCCCACAGCCGGACCCCGCTCCACGCGAACGGCAGCCATACCCGGGCCTC AGCGTCGTCGGGGGCGGTCAGCAGCGCGGGCTGCAACGCGGCGTCCAGCAGCACCGGGTGGATGCCGAACCCGGCCG TGTCCTCACCACCTTCACCGTTGTCACCGCTCTCACCGGCATCGTCGGGCAGCGCCACGTCGGCGACCAGGTCGGCG CCGTCCCGCCACAGCGCCCGTACGCCGCGCAGCGCGGGCCCGTAGCCGTAGCCGCGCCGCTCGGCCCGCTCGTACAG CTCCGCCGGATCCAGGGCCTCGGCGCCCGCGGGCGGCCACGGGACGACCGGCGTTCCGGCCGCCGCCGGGCCGGACC GGCCCCCGGGGACCAGCGACCCCACCGCGTGACACGTCCATTCCCCACCGGTGCCGACACCGCCACCGGCGTCGGCG GGCCGGGACGAGATCCGTATCCCACGGCGGCCGGACCCGTCCGGCGCCGCGATCGCGACCTGCGCCAACACGCCCCC GGGCGCACCCGACAGCTCCAGCGGCGCACGCAGCAGCAGCTCCTCCAGCGACGCCCCGCCCGCCTCGTCGGCGGCCA GCAGCGCCCAGTCGACCAGGACCGCGCCGGGCACCAGCCCGACACCCGCCACGGTGTGGCTGTTGAGCCAGGCCGTG TCGTCGGGCAGCCGCCCGTTGAGCACCAGGCCGCCGTCGGCCAGTTCGACCGCCGAGGACAGCAGCGGGTGTCCGGC CGGGCGGGTCCCGGCCCCGGCGGCGAGCCGCTCGCGACGGCCCTGGTCCGGCGTCAGCCAGTAACGCCGCCGCTGGA ACGGATAGGTGGGCAGCTCGACCGTACGCGGCCGGTGGCCCTCGCCATCGGCGAACCAGCGGCTCCAGTCGACCGAG AGTCCCGCCGCGAAGCCGTCCCCCAGCGCCCGCGCCACCTGATCCGGGCCGCCCTGGTCCCGGCGCAGCGTGGGCAC GGTGGCCGCCGCGACATCGGCCTCCTCGAAGCACTCCCGTAGCGCGGGAATCAGCACCGGGTGGGGGCTGACCTCGA TGAACACGCGGTATCCGGCGTCCAGCAGGGTGTCGATCGCCGGGGCGAACCGCACCGGCTGCCGCAGGTTGGTGAAC CAGTAGTCGGCGTCCAGCTCCTCCGTCGCCTGCCGGGCGGCGGTGACGGCGGAGTAGAAGGCGGCGCCCGAGGCGCG TGGGGCGAGGTCGGCCAGGTCGGCGCGGATCGTGTCGGCGAGCAGGTCGACCTGCTCGCCGTGCGAGGCGTAGTCCA CGTCGATCGTCCGGGCCCGGTGGCCGGCCGCCTCACACGCGGCCACCACCGCGGCGACCTGGTCGGCGGGCCCCGAC ACCACCGTGGAGTACGGCCCGTTGAACACCGCCACCGTCACCTCGGCGGCCGCCGCGCCCCGGTCCGCGAGGAAGTG CTCGGCCTCCTCGGCGCTCAGCGCGACCGAGGCCATGGTGCCGTGGCCGGCGAGCGCCCGCAGGGCCCGGGAGCGGA GCGCCACCACCCGGGCGCCGTCCCGCAGGGACAGGCCCCCGGCGACGCAGGCGGCGGCGATCTCGCCCTGCGAGTGT CCGACCACCGCGGCGGGCGTCACGCCGAAGCTCTCCCAGACAGCGGCGAGCGACACCATCACCGCCCACAGGCAGGG CTGGAGGACATCGACCCGGGACGGCTCCGGCGCCCCCGGCGCGCCGCGCAGCACGGCGGTGAGCGACCAGTCGACGA ACTCGCCGAGCGCCGCCTCGCACTCGGCGACGCGCGCGGCGAAGACGGGGGAGGAGTCAAGCAGTTCCACGCCCATG CCGCGCCACTGCGAACCCTGCCCGGGGAACACCATGACCGGGCCTGGCGCGGCCCCCGCGGAGACGGCGACGGCCGG CGGTCCGTCCGCCACCGCCCGCAGTGCGGACAGCAGCTCGTCCCGGTCCGCGCCGCTGACCACGGCGCGGTGCCGGT CCGCCGACCGCGTGGTGGCAAGCGACCACCCCACGTCCACCGGGGAGGGCGAGGCGCCGTCGGACACCCACTCGGCC AGGCGCCCGGCCTGGGCGCGCAGCGCCTCGGCGGTACGCGCGGACAGCACCCAGGGCACCACGCCACCCTCGGAAGA GGTCTCCGTAGCGGGCGATCCAGGCTGCTCAGGGGCTTGTTCGAGGATGAGGTGGGCGTTGGTGCCGGAGATGCCGA AGGAGGAGACGGCGGCGCGGCGCGGTCGGTCGGTGTCGGGCCACTGGGCGGCCTCGGTGAGGAGGCGGACGTTTCCG GTTGCCCAGTCGACGTGCGGGCTGGGCGCGTCGACATGCAGGGTGGCGGGCAGTCGGCCGTGGCGCAGGGCCATCAC CATCTTGATGACGCCCGCGACACCCGCCGCCGCCTGGGTGTGGCCAAGGTTGGACTTGATGGACCCGAGCCACAGGG GCCGGTCCTCGGGGCGGTTCTGTCCGTAGGTGGCGAGGAGGGCCTGGGCCTCGATCGGGTCGCCCAGCGTGGTGCCC GTGCCGTGGGCCTCGACCGCGTCCACGTCCGCCGGGTCCACCCCGGCCGTGGCCAGGGCCTGCCGGATGACGCGCTG CTGCGAGGGGCCGTTGGGGGCGGAGAGGCCGTTGCTCGCGCCGTCCTGGTTCACCGCCGAGCCACGGATGACGGCGA GCACCTGGTGGCCGTTGCGGCGGGCGTCGGAGAGGCGTTCCAGCAGGACGAGCCCGGCACCCTCGCTCCAGCCGGTG CCGTCGGCGGCGGCGGCGAACGGCTTGCACCGCCCGTCCGGGGCCAGGCCGCGCTGCCGGGAGAAGGAGGTGAACAT GCCAGGCGTGGCCATCACCGTGGCCCCGCCCGCGAGCGCCAGGTCGCACTCGCCCTGCCGCAGCGCATGCGCGGCGA GGTGCAGCGCCACCAGCGACGACGAACACGCCGTGTCCACCGTGAGCGCCGGTCCCTCCAGGCCGAGGGTGTAGGCC ACCCGGCCGGAGGCCACGCTCGGTGTGGAGCCGGTCAGCAGA TGCCCGTCGATCTCGGCGGTGGCCTCGTGCAGCCGTGGCCCGTAGTCCACGGCCGTCGCCCCCACGAAGACGCCGGT CGCGCTGGAGCCCAGCGAGGCCGGGGGGATGCCGGCCCGCTCCAGCAGCTCCCAGGAGAGTTCGAGCAGGATGCGCT GCTGCGGATCCATGGCCAGCGCCTCGCGCGGGCTGATCCCGAAGAACTCCGCGTCGAAGTCCCCCGCGTCGTGGAGA AAGCCGCCCCGGCCCGTCGCGCTGCCGCCGCTGCCGCCGCTGCTGTCCGGGTTTCGGCCGAACAGGTCGGCCAGGTC CCAGCCCCGGTCGCCCGGGAACTCACCCACGGCGTCGGTGCCTTCGTCCACCAGCCGCCACAGTGCCTCGCCGGAGT CGGCCCCGCCCGGGTAGCGGCAGCTGACGGCCACTACGGCGATGGGGTCGTCGGCCGTGGCCGACCGGCGCGTCGGG GCGGGGGCGGTGGCCGGCCGCCCGTCGCGGGCCGCGCGCAGCCAGGCGGCCACGGCGTTCGGCGTGGGGTGGTCGAA GGTGAGGGTGGCGGGCAGGGGGATCCGCGTGGCCGTGCTGAGGCGTTCGCTCAGCTCCGTGGCGGCCAGCGAGTCGA AGCCGAGCTGTTTGAAGGTCAGGCTCGGGTCGATGCCGTCGGGGCTGTCGTGTCCCAGCACCAGGGCCGCGTGCGTA CGGACGAGGGCCAGCGGCTCGCCGGGGGAGCCTCCATTGTCGGCGGAGGTGGCGGCCGGGGCGGCGGCCGGGGTGGC GGCCGGGGTGGCGGCCGGGCCCGATGACAGAGCCGGAGCTGCCCGCCGCCCGTCCGCGGCCTCGTCCAGGGCGTCGT TCAAGGCGTCGTCGGGCCAGTGGCGTTCGCGCTGGAAGGCGTAGGTCGGCAGGGGCACCCGCCGGGGCTCGTGCCCG GCGAAGCCCGCCGCCCAGTCGACGGCCACGCCTCGTACGTGCGCCTGTGCCATCGCCGCCACGAAGCTGTGCGCCTC CGGCCGTTCGCGGCGCTGCACCGCCACCGCCGCCGGGCGGGGCGCGTCGCCCGCCCAGGCCGTGGCGCTCTCCCGCG CCATGCCCGTCAGCGAGGCGTCGGGGCCGAGTTCCAGGAAGGTGGTGACCCCCGCGTCGCACAGGTGCCGCACACCG GAGTCGAACCGCACGGCCTGCCGCGCGTGGTCGGCCCAGTAGTCGGGCGAGCGCAGCTGGGCGTCGGTGGCCGGCCG GCCGGTCACGTTCGACACGACGGGGATGGCGGGCGCGGCGAAGGCGAGCCCGGCCGCGACCGCGCGCAGCTCCCCGA GCACGCCGTCCAGGTGCGGGGAGTGGAACGCGTGGCTGACCTTCAGCACGGTGGCCTTGCGGCCCTGCTCCCGCCAC GCGGCGGTGGCCGCCACCACGGCGTCGCGGTCGCCGGAGACCACCACGGAGGCGGGTGCGTTGACGGCCGCGAGGCC CACCCGTCCGCCGTGTGCCTCCAGCGCGTGCCCGGCCTCCTCGGCGGTCGCCTGCCAGGCGGCCATCGCCCCCGGGA TGGCGACCGACTGCATGAGGCGGCCCCGGGTCGCCACCAGCGCGCAGGCGTCGGGCAGCGAGAACACCCCGGCCACG TGCGCGGCCGTGATCTCGCCGACCGAGTGGCCCATCAGATGGTCGGGCGTGAGGCCCCACGACGTCACCAGGCGGTA GAGGGCCACCTCGAACGCGAACAGCGCGGGCTGGGTGTACTCGGTCCGGTCCAGCGGAGCCTCGGCACCCGGCCTCG CCTCGGCGAACATGACGTCCAGCAGCGGGGTGTCGAGCCAGGGGTCCAGATGCGCGCACACTTCGCGCAGCGCGGCG TCGAAGACCGGGTACGCCGCGCAGAGCTCGTGGCCCATGCCGGGGCGCTGGCTGCCCTGGCCGCTGAACAGGAACGC GACCCGGCCGCCGCCCGCGCTGCCACGGAACACCCCCGTCGCGTCCGCCCCGGCGGCCAGCGCGTCGAGACGACCGA GGAGTTCACCGCCGTCGGTGCCCGTGCCCAGGACGACGGCCCGGTGGGGGAACGCCGTCCGCGTCGTGGCGAGCGCG TGGCCGACGTCCACCGGGTGCAGCCCGGGGTGTGCGCGCAGGTGTTCGCGCAGCGCGGCGGCCTGGGCCGGCAGGGC TTCCGCGCTCGCCGCCGAGACGACCCACGGCGTGACGGGCGGGGCGGGGCGCGCGGACGCGTCCCCGGGCGGTGGCG GCGTCGGCCGCGCCGATGCCGTGGCCGTGGCCGTGGCGGGCGGCTCGGCCAGGACGACATGGCAGTTGGTGCCGCCC ATGCCGAAGGAGCTGACCCCGGCGGTGAGCCGCCGCCGCTCGTCCGGCCAGGGCCCGAGCTCGGTCTGGACGCGCAG GCCGAGCTCGTCCAGCGGGATGTCCGGGTTGGGGGTGGCGAAGTTGAGACTCGGCGGAAGCCGCCGGTGATGGATGC TCAGGGCCGTCTTCAGCAGCCCCGCGATGCCCGCGGCGGCCTCCAGGTGGCCCACGTTGGTCTTGACCGAGCCGACC CGCAGCGGGGTGTCCGCCGGGCGCCGCCCGCGGAGCGCGGCGCCGAGCGCGGCGGCCTCGATCGGGTCGCCGACGGG TGTGCCGGTGCCGTGGAGTTCGACGTACTGCGGTTCGGCGGGCGCCACTCCGGCCGCCTGCCACGCCTGTCGCACCA CATCCGTCTGCGCCTCGGCGCTCGGCCGGGTCAGCCCGTCGGTGGCGCCGTCGTTGTTGACGGCGCTGCTCTCGATC ACGCAGTACACCGGGTCGCCGTCCCTGACGGCCGCGGCCAGCGGTTTGAGGAGCAGCATGCCGCCGCCCTCGCCGCG TACGAAGCCGTTGGCGCGCGCGTCGAAGGTGTAGCAGCGGCCGTCCGGTGAGAGGCCCTGGAACTGGGCCTCGGCCA CCCGCATGCTCTCCTCGGCGAGGATCAGGTTCACCCCTCCGGCCAGCGCCAGATCGGCCTCCCCCCGGCGCAGGCTC TCGCAGGCCAGGTGCACGGCGACCAGCCCGGAGGACTGCGCCGCGTCCACCGTGAGACTCGGGCCGCGCAGGCCCAG GCGGTGGGAGATCCGGTTGGCGATGATGCCGCGGTGCAGACCCGTCATCGTGTGACGGGTGATGTCATGGGGCCGGT GCCGGTGGAGGAGCGTGGCGTAGTCGTCCCAGATGGCGCCGGTGAACACGGCGGTGCGGCCGTCCGCCAGGGTGTCG GGGCCGAAACCGGCGTCCTCCATGGCCTCCCAGGCCAGTTCGAGCATCAGCCGCTGCTGTGGATCCATCGCCACGGC CTCGCGCGGCGAGATGCCGAAGAACCGCGCGTCGAAGGTGTCGACGCGCTCCAGGAGCCCGGCGAAGTACGGCGCGG AGCCGGGCCGCGCGGGCAGGCGGCCGGCCGGTACGGGAACGACGGCGCTCCCACCCGTGCGCAGCAGATCCCAGAAC GCCTCCGGGTCGGGGGCGTTCGGCAGTCGACAGGCCAGGCCCACGATC GCGACGGGAGACTTCGCCTGCGGTGAGCGGGTCGGGCTGCTCCCGGCCATGAAACCCCCATACCTGAATTGCAAAAC CGCTGGTTATGGCTCGCGACATGCCCCGAACGGTGTTCATTCTGGGAATCCGGCCTAAAGTTGATCTAACCGAAAGC ACTCCGGGCGGGTTGACAACGGGGTTGACCGCGCCGGGGTAAAGATCGCGTCGATGATCTGCCGCACCGGGGCCCGA CACCCGGTGATCGTGGCCCGAGCAACCGGGGCGGCGACGGTCACCGGTCGATGAATCGCCGTATATGCCCATTTGGT TGTCGGTAGATCGACCCTCACACTGTCGCGCCGTGCGCCGTTAGGATGCGGCGATGCTGCTTTCTCTGCGAGCTCTG TTGCCGATTGTTCCGCCGTCAGCGCCAATCGGCGTCTCCGGCGCCGGCCGCAGTGGTGCACGATGATTCAGCGCACC CGCCGATCGCAAAGCGTCCGTCGATCTTCATAGCCGAGAAGCCCACTCTCACCCGACGCCAATATCGAACTGGGGGC AATGTTGTATCCGCGTGTAAAGTGCAGACGTGGCTTCGTTCTGGCTAAGCTGTCCACGGCGTGGGTAAGACGTCACC ACTGAGGTCCGAAGCGCTACCATAAAGGCTTCGGGCCGTCTTGTGTATGGTCGACAGGTTCGAACATAAAATCGGGG TGAATTTCGAATGCTGACCAAATTCGAACGCAATGGTGAGTGCCCCAGGGTCGGCATGGCGCCGACCACGGTGGGGG GTCGCGCGTCATGAGGTACGAGATACTCTGCCCGCTGCGCGTCGTGGACGGCACGTCCACGACCTCGCTCAGCGCCC GGAAGATCGAGGTGCTGCTGGCCGCTCTGCTGGTGCGTGCGGACGACGTGGTGAGCAGTGAGGATCTCGTGAAGGAG ATCTGGGGTACTCGCGCACCGCGTCAAGCCGTGGCCACGCTCCACGTCTACGTGTCCCAGCTCCGCAAGTTCCTCAG GCGCTCCGCCGACCGGACCGAGAGCCCCATCGTGACCGCTCGCAGCGGCTATGTGCTGCGTCTCGGCGCGGACGAAC TGGACTTCCACGTCTTCCAGGACCTGGTGCGCCAGGGCCGGTCCGCGCGGCAGGCCGGGCGCACCCAGGAGGCGTGC GAGGCGTACGAGGAGGGGCTCGACCTCTGGCGCGGCCCGGTGCTCGAGGACCTGCGTGACGGGGCCATCGTCAACGG CTTCGTGTGCTGGGCCGAGCAGGCCCGGCTGGAATGTGTCGAGTCGTTCGTGGACGCCGGCCTGGAAATCGGCCGCC ACCGTGAATTCGTCAGCTATCTCTATGCGCGGATCGAGGAGCTTCCGCTGCACGAGGCGTTCTACCGGCAGCTCATG CTGGCGCTCTACCGTTCGGGGTGCAGAGCGGACGCCCTGAGCGTCTATCAGTCGGCGCGGACGGTCCTGGGGGAGGA ACTCGGGCTGGAGCCGTCCGCCACTCTCAAGCGGCTGCATCACGCCGTGCTCGTCGATGCGCCGGAGCTCGAAGCCG CCTAGCCGGCGAAGTGGTGCGGAGTAGCGCCCAGGTAAATTCAGTGCCGACCGAGTGCAGGGGGAAATTCAGTGCGG TACGAGATTTTAGGACCTCTCCACGTCGTCGATGAAAAGGGCAGTTCACTGCTCAGAGCGCAGAAACTCGCCACGGT TCTCGCGGTTCTGCTGATCCGCTCGGATCAGGTTGTCGTGGCGGATCAGCTCACGAGGGAGATCTGGGGCGAGCTGC CCCCGCGGCGGACCACCGCGAGCATCTACGTCTGCATTTCGCGGCTCCGCAAGTTCCTGAGCCGGCCCGGCCAGCCG GATCCCATCGTCACGCGTCCCGGTGGATATATGCTGCGCCGGGCGTCGAGCGAATGTGATTTCGATGTCTTCCAGCG TTTGGTCGCGGACGGCCGCAAGTGCCTCAGGGGCGGGGAGCCCGCGGCGGCGGCCACCCGCTTCGAACAGGCCCTGG CGCTGTGGCGGGGCCCGGCGTTCGGCAATGTCCAGCCGGGACCGATCATGGCGGGCTTCCTCAAGCGGCTGGCCGAG ATGCGCATCGAGTGCGCGGAGATGTCCGCGGAGGCCCAGCTCGAACTGGGCCGCCACCGGGAGCTGGTCGGCCGGCT GCGGTCCCTCGTCGACGAGTTCCCGCTCCGGGAGACGTTCTACGGCCAGCTGATGCTGGCCCTCTACCGCTCCGACC GGAAGTCCGAGGCGCTCGGCATATACGAGTCCGCCCGGCGCGTCCTCGACCGCGAGCTGGGGCTCGAACCCGGGCGG AAGCTCAGAGAGCTCCGCCACACCGTGCTCGCCTCGACCTGACGGAGCGGGCCGACGGTGACCCGGGCCCCGGCCCC GTCCGTCCGTCTGTCGGTCCGTCTGTCCGGCCGGCCCGAACTCGCCGGGGGCCTCCCGCGCACGGCGCGGGCGAGCC CACCGGCGATGATTCCGGGCCCCGGGTCATTCGGCGACGTAGGTCCGGCGAATCCACTCGAAGACCCGCAGGTGCTG CCCCTGGTGCTCCTCGAACCGAGCTGTGGGCCGGAAGTGTTCGTAGGCGTTGCCGACGAGGAGCTTCAAGGTCGAGT CCGTGTCGTGGACATACCGCGCGCACGACTGATCGGTCAGCTGCGTGGGACCCCCGCGCAGGATGGCATTGGGTGCG GATGCGGACATAAGCGACTGCATTCCCTTCGCTATACATTTCAAGAGAAGGTTGGCATGAGGTGAAAGCGCGCCGAT GTGTGACAAACTCCTTGTTCACAAAGGTCTTGTGGGGGCCCGACTTGGGTGAGATGGCAGATCGCCCCGACCTGCTC CCCCGGACCCGCTGCGCCTGGTCCAGCGTAGACGCGCAGGGTCATGCGGTGTGAGGGTCGTTCGACCGACAAAACGA CGACGAAACGACCGACAAAGAGGTTCCCTCCCTCACGGATCGTGAGGTTCCCGCCATCGCCGTGTCACACAGACGGT CGCCGAGGATGGATGACTCATGCCGCTTCCCTCACTTCCGCTCCGCGTCCAGCTGCCGGGCCTGAGGCGCTTACCCG AGTCATCCGGGCCCTGGGCGCGCGAATACGTGGCGTTCAACCGGCAGTTGGCGCTCTACGGCCGCGCCGGGGTGATG AGCGCCTGCGCGGTGCTGGCCGTGGTGGGCATGCCGGCGGGGAAGATGCCGGTCGCGGCGGCGATCGCGTCCGCCGC GTTCGCCTGGAGCTGGATCCACCTGTGGCTCGCCAACGCCTCGACGTTACGGCCGGGGCGGCTGCTCGCCCTCGACC TGGCGGTCATGACCGCGTTGTGTCTCAGCCAGCGGCTCACCGTCCCCGACGCGCCGACCGGCCACGGCAGCACCTGG ATCCTGGTGTCCCTCAACTTCACGGCCGTGGCCTACCAGCTCATGCAGCCGCCCCTCGTCGCGATTCTGGCGACGCT CTGGCTGTGCTGCGCGGACCTGGCGGGCGCCGCGCTGCACCCGCGCCAGGAGTTGACCGACGTCGC GTACAGCCTGGGCTGGGTGCTGGTGAACATCGTCCTGGCCCAGGCCGTGGTCCGACTGGTGTTCGGCGAGAGCCGGA CGGCCGACGAGGCCGCCGACCGGGCCGCGCGGGCGCGCCGGCAGGGCGAGGCGGCGGAGGCCCGGTGCGCGGCGGAG CGGGAACACCTGGCCGCGCTCCACGACACCGCCTGCGCGACCCTGCTGATCGCCTCGGCGCCATGGGCGTCGATGCG GCCGGAGACGCTGCGCGCCCAGGCCGCGAGCGACCTCGTCCGACTGCGCACCGAGCAGTCCGCCGCGGGGGAGGTGG ACCTGACCGTCGAGCTGGCCGCCGAGATCGCCGCGCATCCCCTCCGGGCCACCACGCTGTTCGACCCGGATCTCGGC ACGGTCTGGCGCCCCGCCGCGGCGGCGCTGCGCGGCGGCCTCGGCGAGGCGCTGCGCAACGTGGAGCGCTACGCGGG GGTGGGCGCCGTGCGGGTCACGGCGGTGCGCACCCGCCACACGGTGGTCGTGACCATCACGGACAGCGGGGTGGGGT TCGACACCGGGCGCGTTCCCGCCCAGCGGATCGGGATCCAGCGTTCCATCCGCGGGCGGATGGCGAGCGTGGGGGGC CGCGGCACCGTGGAGTCCCGGCCGGGGTGCGGCACGACGGTACGTCTGGAGTGGCCGCGTGCCTGAGGACCTGCGTC AGCTGATCACCCGTCACCAGCTCCGCGGCATCCGCGCCGGGGCACTGGTCATCCTGGTCACCCTCCACTTCGCACTC GCGCTGCCCGGCCTCCTGAGCAGCCTGGAGGAGTACCGGCACCCCTGGGTCCCCGTGACCGCCTACGGACTGCTCAC CGTGATTCTCGGCGGCAGCGTCCTTCTCGGCCTCGACGGGCGCCCCTGGCCGCGCGGCTGGGTGCCGACGGCGCTGG CCGGCGCCTTCGCCGCCTCGGTGGCCGCCACCACCCAGGTGCCGGCGAGCCAGTACTTCGTCGGCGCCTCCCACTGG TCCTTCACCCTCGTGGGCTGGTTCGCCGTCGTCCTGCTCGGCCACCGGGGACCCATGGCGTTCGGCGCCTTCATCGG CTGCCATCTGGCCGTCAACACCGCCCAGTTGCTGACGGCCGACGTCCCCTCGCGGCCCATCGCCGCGGGCATGGCCG CGAGCGCCCTGTCCGCCTGCTGCTTCCAGATGACCATCGCCGCCGGCGCGAAGCTGCTGCTCAACAGCAGCACCGCC ATCGGGGAGGCCCTCCAGGTGCAGGAGCGGGTGCGCACCCGCATCGCCGTCGCCACCCAGATCCAGACGGACCAGCG CCGGCGCTACGCCGAGCTGAACGCGACCGTGCTGCCCCTGCTCACCGGCCTCGCCAACGGCTCCCTGGACCCACGGG ACGACGAGGTGCGGCGGGCCTGCGCGCTGGAAGCCGCCCGGCTGAGGCGGCTGTTCGCGGAGAGCGACTGTACGTTC GACCCGCTGGTCCACGAGATGCGGGCCTGTATCGACGTCGCCGAGCGCAACGGGACCACCGTGCAGCTCGCCGTCCG CGGCGACCCCCTCGAACTGCCGCTGCCGCTGCGCCGCGCGCTCCTGGACCCGGTCATCGCCGCACTGGCCGCCGCCC GGCAGACGGCGCGCGTCACCGTGGTGCGCGGCGAGAATCAGGTACGCGTGGGCGTGGTGGTGGACGCGCTGCGGGAT CGGCTGCCCGAGCCGGCCGCGAGCGGGATCCGGGTGCGCACGGTCCGCGCGGAGGACCGTCTCCTTGTGGAGGCCGC GTGCCGGATCACAGCTCGTCCAGCGCGATCAGACCGTCCTGAACCGCCCTGGCCACCAGCTTGGCCTTCGTGGTCGC CGGGCGGCCGGCGTTCGCGTACTTGATCCGCACCCGGTCCAGATAGGTGTTCACCGTGCGCTCCGAGATCCCCAGGC TCTTGGCCACCATGGCCTTCGACTCGGACTGGAACCACTCCACCAGCACCTCCACCTCCCGCACGGACAGCACCGGG CGGCTCTGCCGGGTGTCGCTGCCGAACGCGCCGGCCAGCGACGGGGGTGTGTAGGGGATGTCCGCGGCGGCGGCCTG GATCGCCTTGACCAGGTGCCGCTGCCCCTCCGCCTTGGTGATGTAGGTGGCCGAACCCAGGTCCATGCAGGTCAACG CCGCCTCCTGGCTGTCCCGCATCGTGTAGACGACGACCTTGCGCTCCTGCGCGACCAGCACCTGCAACTCCTCGTAC GCGGGTCGCCCGTTCTGCAACAGCAGGTCCATGACCACGACGTCCGCGCCCCGGCCCGGACCGCAGAGGGCCACCGA AAGCTCCGACCCCTCGGCCACCACCTTGATCGGCACGTGGGCGGCGGCACACCAGGCCGCGATGCCCGCGCGTACCA CGGGGTGATCATCGACCAACACGACGCTGAGGGCCAT(SEQ ID NO：35)
By taking the biological synthesis gene cluster of the streptomycete as an example, biological synthesis gene cluster is explained, the biology is closed Include I Linear polyketide synthases netic module, glycosyl synthesis related gene module and modifier module into gene cluster.Below Each module of biological synthesis gene cluster to the streptomycete is described in detail, specific as follows：
Wherein, I Linear polyketide synthases netic module includes 2A1,2A2,2A3,2A4,2A5,2A6,2A7,2A8, 2A9,2A10 and 2A11 totally 11 genes, it is specific as follows：
Embodiments in accordance with the present invention, 2A1 gene code SEQ ID NO：Amino acid sequence shown in 1.It is specific as follows：
Embodiments in accordance with the present invention, 2A1 genes are located at the gene cluster nucleotide sequence 92698- of the streptomycete At 101334 bases, length is 8637 base-pairs, encodes polyketide synthases, and length is 2878 amino acid.
Embodiments in accordance with the present invention, 2A2 gene code SEQ ID NO：Amino acid sequence shown in 2, it is specific as follows：
Embodiments in accordance with the present invention, 2A2 genes are located at the 85970-92683 base of gene cluster sequence of the streptomycete Place, length is 6714 base-pairs, encodes polyketide synthases, and length is 2237 amino acid.
Embodiments in accordance with the present invention, 2A3 gene code SEQ ID NO：Amino acid sequence shown in 3, it is specific as follows：
Embodiments in accordance with the present invention, 2A3 genes are located at the gene cluster nucleotide sequence 73776-85781 of the streptomycete At individual base, length is 12006 base-pairs, encodes polyketide synthases, and length is 4001 amino acid.
Embodiments in accordance with the present invention, 2A4 gene code SEQ ID NO：Amino acid sequence shown in 4, it is specific as follows：
Embodiments in accordance with the present invention, 2A4 genes are located at the gene cluster nucleotide sequence 62077-73755 of the streptomycete At individual base, length is 11679 base-pairs, encodes polyketide synthases, and length is 3892 amino acid.
Embodiments in accordance with the present invention, 2A5 gene code SEQ ID NO：Amino acid sequence shown in 5, it is specific as follows：
Embodiments in accordance with the present invention, 2A5 gene clusters are located at the gene cluster nucleotide sequence 50179- of the streptomycete At 62031 bases, length is 11853 base-pairs, encodes polyketide synthases, and length is 3950 amino acid.
Embodiments in accordance with the present invention, 2A6 gene code SEQ ID NO：Amino acid sequence shown in 6, it is specific as follows：
Embodiments in accordance with the present invention, 2A6 genes are located at the gene cluster nucleotide sequence 45212-50158 of the streptomycete At individual base, length is 4947 base-pairs, encodes polyketide synthases, and length is 1648 amino acid.
Embodiments in accordance with the present invention, 2A7 gene code SEQ ID NO：Amino acid sequence shown in 7, it is specific as follows：
Embodiments in accordance with the present invention, 2A7 genes are located at the gene cluster nucleotide sequence 33430-38406 of the streptomycete At individual base, length is 4977 base-pairs, encodes polyketide synthases, and length is 1658 amino acid.
Embodiments in accordance with the present invention, 2A8 gene code SEQ ID NO：Amino acid sequence shown in 8, it is specific as follows：
Embodiments in accordance with the present invention, 2A8 genes are located at the gene cluster nucleotide sequence 7986-19214 of the streptomycete At individual base, length is 11229 base-pairs, encodes polyketide synthases, and length is 3742 amino acid.
Embodiments in accordance with the present invention, 2A9 gene code SEQ ID NO：Amino acid sequence shown in 9, it is specific as follows：
Embodiments in accordance with the present invention, 2A9 genes are located at the gene cluster nucleotide sequence 25758-28115 of the streptomycete At individual base, length is 2358 base-pairs, encodes polyketide synthases, and length is 785 amino acid.
Embodiments in accordance with the present invention, 2A10 gene code SEQ ID NO：Amino acid sequence shown in 10, it is specific as follows：
MEPIEESTGD SRLAERLATA TPEEREQLLR GLVLRHAAEI LEAPELDEES NFVENGLTSL 60
RALQLSKALM NDTGLEAPLV AIVEHPTPTL LGKFLSETYE ADAA (SEQ ID NO：10)
Embodiments in accordance with the present invention, 2A10 genes are located at the gene cluster nucleotide sequence 32185- of the streptomycete At 32499 bases, length is 315 base-pairs, encodes the ACP albumen of polyketide synthases, and length is 104 amino acid.
Embodiments in accordance with the present invention, 2A11 gene code SEQ ID NO：Amino acid sequence shown in 11, it is specific as follows：
Embodiments in accordance with the present invention, 2A11 genes are located at the gene cluster nucleotide sequence 19227- of the streptomycete At 25730 bases, length is 6504 base-pairs, encodes polyketide synthases, and length is 2167 amino acid.
Wherein, glycosyl synthesis related gene module includes 2G1,2G2,2G3,2G4,2G5,2G6,2G7,2G8 and 2M totally 9 Gene, it is specific as follows：
Embodiments in accordance with the present invention, 2G1 gene code SEQ ID NO：Amino acid sequence shown in 12, it is specific as follows：
Embodiments in accordance with the present invention, 2G1 genes are located at the gene cluster nucleotide sequence 40030-40842 of the streptomycete At individual base, length is 813 base-pairs, encodes Cori ester：TTP thymidine based transferases, length is 270 amino Acid.
Embodiments in accordance with the present invention, 2G2 gene code SEQ ID NO：Amino acid sequence shown in 13, it is specific as follows：
Embodiments in accordance with the present invention, 2G2 genes are located at the gene cluster nucleotide sequence 40839-41831 of the streptomycete At individual base, length is 993 base-pairs, encodes dTDP glucose -4,6- dehydratases, and length is 330 amino acid.
Embodiments in accordance with the present invention, 2G3 gene code SEQ ID NO：Amino acid sequence shown in 14, it is specific as follows：
Embodiments in accordance with the present invention, 2G3 genes are located at the gene cluster nucleotide sequence 41895-43199 of the streptomycete At individual base, length is 1305 base-pairs, encodes NDP-D- glucose -3,4- dehydratases, and length is 434 amino acid.
Embodiments in accordance with the present invention, 2G4 gene code SEQ ID NO：Amino acid sequence shown in 15, it is specific as follows：
Embodiments in accordance with the present invention, 2G4 genes are located at the gene cluster nucleotide sequence 43196-44194 of the streptomycete At individual base, length is 999 base-pairs, coding NDP-D- glucose -4,6- dehydratases, NDP-D- glucose -4- isomerases, NDP-D- glucose -4- reductases length is 332 amino acid.
Embodiments in accordance with the present invention, 2G5 gene code SEQ ID NO：Amino acid sequence shown in 16, it is specific as follows：
Embodiments in accordance with the present invention, 2G5 genes are located at the gene cluster nucleotide sequence 38479-39750 of the streptomycete At individual base, length is 1272 base-pairs, and encoding glycosyltransferases, length is 423 amino acid.
Embodiments in accordance with the present invention, 2G6 gene code SEQ ID NO：Amino acid sequence shown in 17, it is specific as follows：
Embodiments in accordance with the present invention, 2G6 genes are located at gene cluster nucleotide sequence 3445-4629 of the streptomycete At base, length is 1185 base-pairs, and encoding glycosyltransferases, length is 394 amino acid.
Embodiments in accordance with the present invention, 2G7 gene code SEQ ID NO：Amino acid sequence shown in 18, it is specific as follows：
Embodiments in accordance with the present invention, 2G7 genes are located at gene cluster nucleotide sequence 1169-2848 of the streptomycete At base, length is 1680 base-pairs, and encoding glycosyltransferases, length is 559 amino acid.
Embodiments in accordance with the present invention, 2G8 gene code SEQ ID NO：Amino acid sequence shown in 19, it is specific as follows：
Embodiments in accordance with the present invention, 2G8 genes are located at the 1-1116 alkali of gene cluster nucleotide sequence of the streptomycete Ji Chu, length is 1116 base-pairs, encodes endoglucanase, and length is 371 amino acid.
Embodiments in accordance with the present invention, 2M gene code SEQ ID NO：Amino acid sequence shown in 20, it is specific as follows：
Embodiments in accordance with the present invention, 2M genes are located at the gene cluster nucleotide sequence 44191-45117 of the streptomycete At individual base, length is 927 base-pairs, encodes transmethylase, and length is 308 amino acid.
Wherein, modifier, including 2O, 2I, 2E, 2P1 and 2P2 totally 5 genes, it is specific as follows：
Embodiments in accordance with the present invention, 2O gene code SEQ ID NO：Amino acid sequence shown in 21, it is specific as follows：
Embodiments in accordance with the present invention, 2O genes are located at the gene cluster nucleotide sequence 30711-32147 of the streptomycete At individual base, length is 1437 base-pairs, encodes epoxide hydrolase, and length is 478 amino acid.
Embodiments in accordance with the present invention, 2I gene code SEQ ID NO：Amino acid sequence shown in 22, it is specific as follows：
Embodiments in accordance with the present invention, 2I genes are located at the gene cluster nucleotide sequence 29661-30569 of the streptomycete At individual base, length is 909 base-pairs, encodes ketone steroid isomerase, and length is 302 amino acid.
Embodiments in accordance with the present invention, 2E gene code SEQ ID NO：Amino acid sequence shown in 23, it is specific as follows：
Embodiments in accordance with the present invention, 2E genes are located at the gene cluster nucleotide sequence 32547-33428 of the streptomycete At individual base, length is 882 base-pairs, encodes EH, and length is 293 amino acid.
Embodiments in accordance with the present invention, 2P1 genes have SEQ ID NO：Amino acid sequence shown in 24, it is specific as follows：
Embodiments in accordance with the present invention, 2P1 genes are located at gene cluster amino acid sequence 6809-7960 of the streptomycete At base, length is 1152 base-pairs, and Codocyte cytochrome p 450 albumen, length is 383 amino acid.
Embodiments in accordance with the present invention, 2P2 gene code SEQ ID NO：Amino acid sequence shown in 25, it is specific as follows：
Embodiments in accordance with the present invention, 2P2 genes are located at the gene cluster nucleotide sequence 28182-29462 of the streptomycete At individual base, length is 1281 base-pairs, and Codocyte cytochrome p 450 albumen, length is 426 amino acid.
Embodiments in accordance with the present invention, the biological synthesis gene cluster is further included：Regulatory gene module, the regulatory gene Module includes：2R1 genes, 2R2 genes, 2R3 genes, 2R4 genes, 2T1 genes, 2T2 genes, 2T3 genes, 2T4 genes and 2T5 Gene, the sequence information of said gene is specific as follows：
Embodiments in accordance with the present invention, 2R1 gene code SEQ ID NO：Amino acid sequence shown in 26, it is specific as follows：
Embodiments in accordance with the present invention, 2R1 genes are located at the gene cluster nucleotide sequence 103016- of the streptomycete At 103636 bases, length is 621 base-pairs, and coding regulatory protein, length is 206 amino acid.
Embodiments in accordance with the present invention, 2R2 gene code SEQ ID NO：Amino acid sequence shown in 27, it is specific as follows：
Embodiments in accordance with the present invention, 2R2 genes are located at the gene cluster nucleotide sequence 102065- of the streptomycete At 102829 bases, length is 765 base-pairs, and coding regulatory protein, length is 254 amino acid.
Embodiments in accordance with the present invention, 2R3 gene code SEQ ID NO：Amino acid sequence shown in 28, it is specific as follows：
Embodiments in accordance with the present invention, 2R3 genes are located at gene cluster nucleotide sequence 5548-6558 of the streptomycete At base, length is 1011 base-pairs, and encoding transcription regulatory factor, length is 336 amino acid
Embodiments in accordance with the present invention, 2R4 gene code SEQ ID NO：Amino acid sequence shown in 29, it is specific as follows：
Embodiments in accordance with the present invention, 2R4 genes are located at gene cluster nucleotide sequence 4589-5539 of the streptomycete At base, length is 951 base-pairs, and encoding transcription regulatory factor, length is 316 amino acid.
Embodiments in accordance with the present invention, 2T1 gene code SEQ ID NO：Amino acid sequence shown in 30, it is specific as follows：
Embodiments in accordance with the present invention, 2T1 genes are located at the SEQ ID NO of the streptomycete：Gene order piece shown in 35 Section is outer, and length is 1428 base-pairs, encodes the integrated transfer protein of film, and length is 475 amino acid.
Embodiments in accordance with the present invention, 2T2 genes have SEQ ID NO：Amino acid sequence shown in 31, it is specific as follows：
Embodiments in accordance with the present invention, 2T2 genes are located at the SEQ ID NO of the streptomycete：Gene order piece shown in 35 Section is outer, with SEQ ID NO:Nucleotide sequence shown in 36, length is 687 base-pairs, encodes ABC transfer factor, length It is 228 amino acid.
Embodiments in accordance with the present invention, 2T3 gene code SEQ ID NO：Amino acid sequence shown in 32, it is specific as follows：
Embodiments in accordance with the present invention, 2T3 genes are located at the gene cluster nucleotide sequence 106595- of the streptomycete At 107239 bases, length is 645 base-pairs, encodes the bi-component feedback regulation factor, and length is 214 amino acid.
Embodiments in accordance with the present invention, 2T4 gene code SEQ ID NO：Amino acid sequence shown in 33, it is specific as follows：
Embodiments in accordance with the present invention, 2T4 genes are located at the gene cluster nucleotide sequence 105490- of the streptomycete At 106689 bases, length is 1200 base-pairs, and encoding chemical acceptor, length is 399 amino acid.
Embodiments in accordance with the present invention, 2T5 gene code SEQ ID NO：Amino acid sequence shown in 34, it is specific as follows：
Embodiments in accordance with the present invention, 2T5 genes are located at the gene cluster nucleotide sequence 104394- of the streptomycete At 105497 bases, length is 1104 base-pairs, encodes bi-component sensor group histidine kinase, and length is 367 amino Acid.
With reference to Fig. 1, embodiments in accordance with the present invention, along 5 ' -3 ' ends of gene, 2T2 genes, 2T1 genes, 2G8 genes, 2G7 genes, 2G6 genes, 2R4 genes, 2R3 genes, 2P1 genes, 2A8 genes, 2A11 genes, 2A9 genes, 2P2 genes, 2I bases Cause, 2O genes, 2A10 genes, 2E genes, 2A7 genes, 2G5 genes, 2G1 genes, 2G2 genes, 2G3 genes, 2G4 genes, 2M Gene, 2A6 genes, 2A5 genes, 2A4 genes, 2A3 genes, 2A2 genes, 2A1 genes, 2R2 genes, 2R1 genes, 2T5 genes, 2T4 genes and 2T3 genes are sequentially connected in series.
Furthermore, it is necessary to explanation, in the present invention, " series connection " is only represented between the order of connection of gene, neighboring gene Be directly connected to, or in the presence of other genetic fragments or junction partial sequence exist it is overlapped.
Some embodiments of the invention, 2T2 genes, 2G7 genes, 2R4 genes, 2R3 genes, 2P2 genes, 2G5 bases Cause, 2G3 genes, 2G4 genes, 2M genes, 2A6 genes, 2A5 genes, 2A4 genes, 2A3 genes, 2A2 genes, 2A1 genes, 2T5 Gene is identical with the expression direction of 2T3 genes.
Some embodiments of the invention, 2T1 genes, 2G8 genes, 2G6 genes, 2P1 genes, 2A8 genes, 2A11 bases Cause, 2A9 genes, 2I genes, 2O genes, 2A10 genes, 2E genes, 2A7 genes, 2G1 genes, 2G2 genes, 2R2 genes, 2R1 Gene, 2T4 genes expression direction it is identical.
Microorganism
According to another aspect of the present invention, the invention provides a kind of microorganism.Embodiments in accordance with the present invention, micro- life Thing has foregoing biological synthesis gene cluster.Inventor, can be with height it has surprisingly been found that carry out fermentation process using the microorganism Imitate, simply and at low cost synthesize J1001-2 compounds.
Embodiments in accordance with the present invention, the microorganism is streptomycete.According to a particular embodiment of the invention, microorganism is Streptomyces hygroscopicus hypotype streptomycetes.
According to another aspect of the invention, the invention provides a kind of method for preparing J1001-2 compounds.With reference to Fig. 2, Embodiments in accordance with the present invention, explain to the method, and the method includes：
S100 fermentation process
Embodiments in accordance with the present invention, fermentation process is carried out using foregoing microorganism, the microorganism after being fermented.
According to a particular embodiment of the invention, fermentation medium condition：Soluble starch (30g/L), soybean cake powder (10g/ L), yeast extract (2.5g/L), calcium carbonate (3g/L).Thus, ferment effect is good, and J1001-2 compound productions are high.
According to a particular embodiment of the invention, the time of fermentation process is 7-8 days.
S200 broken wall treatments
Embodiments in accordance with the present invention, broken wall treatment is carried out by the microorganism after fermentation, obtains containing J1001-2 compounds Solution.
S300 is purified
Embodiments in accordance with the present invention, the J1001-2 chemical combination is purified from the solution containing the J1001-2 compounds Thing, obtains J1001-2 compound products.
Some embodiments of the invention, will be spin-dried for after the solution containing J1001-2 compounds, be extracted using ethyl acetate Take, after concentration, use silicagel column crude separation, be further purified with half preparation HPLC obtain target product afterwards.
The method for preparing J1001-2 compounds according to embodiments of the present invention, is carried out at fermentation using foregoing microorganism Reason, can be efficiently synthesized J1001-2 compounds.Then, by microorganism broken wall treatment and separation and purification treatment, can be with height Imitate, simply and at low cost obtain J1001-2 pure compounds.
Some embodiments of the invention, by predictive genes, thus it is speculated that go out the biosynthesis pathway of J1001-2 compounds Such as Fig. 3.On the one hand synthesized by the extension of polyketone synthetic gene cluster catalysis propionyl coenzyme A and methylmalonyl CoA and ultimately formed LCFA chain, in the modification by 2O, 2I and 2E gene and obtains the intermediate product of J1001-2, on the other hand passes through The catalysis of the genes such as 2G1,2G2,2G3,2G4 and 2M obtains glycosyl, then glycosyl is transferred into fat by glycosyl transferase 2G5,2G6,2G7 On fat acid chain, finally acted on by P450 enzymes 2P1 and 2P2 and form J1001-2.
Below with reference to specific embodiment, the present invention will be described, it is necessary to explanation, these embodiments are only explanation Property, and be not considered as limiting the invention.
The solution of the present invention is explained below in conjunction with embodiment.It will be understood to those of skill in the art that following Embodiment is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.Unreceipted particular technique or bar in embodiment Part, (for example write with reference to J. Pehanorm Brookers etc., Huang Peitang etc. is translated according to the technology or condition described by document in the art 's《Molecular Cloning:A Laboratory guide》, the third edition, Science Press) or carried out according to product description.Agents useful for same or instrument Unreceipted production firm person, be can by city available from conventional products, can for example purchase from Illumina companies.
Embodiment 1
Streptomyces hygroscopicus hypotype streptomycetes are inoculated in SFM flat boards, three days to a four days left sides are cultivated It is right.When bacterium colony is formed in SFM culture mediums, choose during single bacterium drops down onto seed culture medium and cultivate three days to four days or so, according to 1% Amount connects bacterium, and to fermentation medium, (composition of fermentation medium is soluble starch 30g/L, soybean cake powder 10g/L, yeast extract 2.5g/L, CaCO33g/L, pH7.2) in.Condition of culture：30 degree, 220 rotating speeds cultivate 7 to 8 days time.
When culture was by the 7th or 8 day, zymotic fluid is collected, high speed centrifugation makes supernatant be separated with mycelium, mycelium is added etc. The broken bacterium of the acetone of amount, ultrasonic 20min rotates acetone, and supernatant is extracted using the ethyl acetate of equivalent with mycelium, is repeated once. Concentrated by rotary evaporation uses silicagel column crude separation afterwards to dry, crude product HPLC detection products is obtained, in view of HPLC results show the bacterium Yield comparison is high, therefore, our samples also to having concentrated have carried out TLC point plates, and are developed the color in iodine cylinder, it will be apparent that point Snap out, dissolved with methyl alcohol, high speed centrifugation takes supernatant to be done mass spectrum and determine the position result of impact point as shown in figure 4, showing the chemical combination Thing is J1001-2 compounds.
Embodiment 2
With Streptomyces hygroscopicus hypotype streptomycetes as research object, research J1001-2 compound lifes The sequence information of thing synthetic gene cluster, specific method is as follows：
1st, DNA is extracted
(1) Streptomyces hygroscopicus hypotype streptomycetes are inoculated in after being cultivated 2 days in TSBY culture mediums, 7000rpm × 5min collects thallines.
(2) thalline of collection is abandoned into supernatant, plus 30ml SET buffer, is broken up, 7000rpm, 5 minutes is centrifuged.Abandon again Supernatant, plus 10ml SET buffer, break up thalline, plus 300 μ l lysozymes (100mg/ml), 37 DEG C water-bath 30-60 minutes, then Plus 100 μ l Proteinase Ks (100mg/ml), 10 μ l RNase (10mg/ml), 37 DEG C of water-baths 30 minutes.
(3) 1.2ml10%SDS is added in the mixture for obtaining to step (2), 55 DEG C of water-baths 2 hours are light every 15 minutes Shake to liquid and gradually clarify.
(4) 4ml 5M Nacl are added in the liquid for obtaining to step (3), is mixed, be cooled to 37 DEG C.Plus 10ml chloroforms, gently It is light to mix 30 minutes, 12000rpm, 15 minutes is centrifuged.
(5) supernatant of step (4) centrifugation is taken, 10ml chloroforms is added again, gently mixed 30 minutes, 12000rpm is centrifuged, 15 minutes.
(6) supernatant of step (5) centrifugation is taken, 0.8 times of isopropanol of volume is added, gently DL is to there is thread DNA.
(7) choose DNA in EP pipes, the ethanol for plus 70% is washed, then outwell ethanol, natural air drying is dissolved in a certain amount of In distilled water, the DNA for obtaining streptomycete is extracted.
2nd, DNA sequence analysis
The genome is delivered into sequencing company carries out gene order-checking, obtains its genomic information, to the gene after assembling Group, the prediction of encoding egg white gene is carried out using GeneMarkS.Go out and J1-001ization followed by antiSMASH software predictions Albumen is in the bacterium encoding gene data in the similar region of compound synthetic gene cluster, and comprehensive J1-001 compound synthesis gene clusters Comparison result is concentrated, J1001-2 synthesis related genes are excavated.The structure of gene cluster is as shown in Fig. 2 be responsible for compound J1001- 2 biosynthesis, the functional analysis of each gene is shown in Table 1.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show The description of example " or " some examples " etc. means to combine specific features, structure, material or spy that the embodiment or example are described Point is contained at least one embodiment of the invention or example.In this manual, to the schematic representation of above-mentioned term not Necessarily refer to identical embodiment or example.And, the specific features of description, structure, material or feature can be any One or more embodiments or example in combine in an appropriate manner.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that：Not Can these embodiments be carried out with various changes, modification, replacement and modification in the case of departing from principle of the invention and objective, this The scope of invention is limited by claim and its equivalent.

Claims (12)

1. a kind of biological synthesis gene cluster of compound, wherein, the compound structure is as follows,
Characterized in that, the biological synthesis gene cluster includes：
I Linear polyketide synthases netic module, the I Linear polyketide synthases netic module includes：2A1 genes, 2A2 bases Cause, 2A3 genes, 2A4 genes, 2A5 genes, 2A6 genes, 2A7 genes, 2A8 genes, 2A9 genes, 2A10 genes and 2A11 bases Cause；
Glycosyl synthesis related gene module, the glycosyl synthesis related gene module includes：2G1 genes, 2G2 genes, 2G3 bases Cause, 2G4 genes, 2G5 genes, 2G6 genes, 2G7 genes, 2G8 genes and 2M genes；And
Modifier module, the modifier module includes：2O genes, 2I genes, 2E genes, 2P1 genes and 2P2 genes.
2. biological synthesis gene cluster according to claim 1, it is characterised in that
The 2A1 gene codes SEQ ID NO：Amino acid sequence shown in 1；
The 2A2 gene codes SEQ ID NO：Amino acid sequence shown in 2；
The 2A3 gene codes SEQ ID NO：Amino acid sequence shown in 3；
The 2A4 gene codes SEQ ID NO：Amino acid sequence shown in 4；
The 2A5 gene codes SEQ ID NO：Amino acid sequence shown in 5；
The 2A6 gene codes SEQ ID NO：Amino acid sequence shown in 6；
The 2A7 gene codes SEQ ID NO：Amino acid sequence shown in 7；
The 2A8 gene codes SEQ ID NO：Amino acid sequence shown in 8；
The 2A9 gene codes SEQ ID NO：Amino acid sequence shown in 9；
The 2A10 gene codes SEQ ID NO：Amino acid sequence shown in 10；
The 2A11 gene codes SEQ ID NO：Amino acid sequence shown in 11.
3. biological synthesis gene cluster according to claim 1, it is characterised in that
The 2G1 gene codes SEQ ID NO：Amino acid sequence shown in 12；
The 2G2 gene codes SEQ ID NO：Amino acid sequence shown in 13；
The 2G3 gene codes SEQ ID NO：Amino acid sequence shown in 14；
The 2G4 gene codes SEQ ID NO：Amino acid sequence shown in 15；
The 2G5 gene codes SEQ ID NO：Amino acid sequence shown in 16；
The 2G6 gene codes SEQ ID NO：Amino acid sequence shown in 17；
The 2G7 gene codes SEQ ID NO：Amino acid sequence shown in 18；
The 2G8 gene codes SEQ ID NO：Amino acid sequence shown in 19；
The 2M gene codes SEQ ID NO：Amino acid sequence shown in 20.
4. biological synthesis gene cluster according to claim 1, it is characterised in that
The 2O gene codes SEQ ID NO：Amino acid sequence shown in 21；
The 2I gene codes SEQ ID NO：Amino acid sequence shown in 22；
The 2E gene codes SEQ ID NO：Amino acid sequence shown in 23；
The 2P1 gene codes SEQ ID NO：Amino acid sequence shown in 24；
The 2P2 gene codes SEQ ID NO：Amino acid sequence shown in 25.
5. biological synthesis gene cluster according to claim 1, it is characterised in that further include：
Regulatory gene module, the regulatory gene module includes：2R1 genes, 2R2 genes, 2R3 genes, 2R4 genes, 2T1 bases Cause, 2T2 genes, 2T3 genes, 2T4 genes and 2T5 genes.
6. biological synthesis gene cluster according to claim 4, it is characterised in that
The 2R1 gene codes SEQ ID NO：Amino acid sequence shown in 26；
The 2R2 gene codes SEQ ID NO：Amino acid sequence shown in 27；
The 2R3 gene codes SEQ ID NO：Amino acid sequence shown in 28；
The 2R4 gene codes SEQ ID NO：Amino acid sequence shown in 29；
The 2T1 gene codes SEQ ID NO：Amino acid sequence shown in 30；
The 2T2 gene codes SEQ ID NO：Amino acid sequence shown in 31；
The 2T3 gene codes SEQ ID NO：Amino acid sequence shown in 32；
The 2T4 gene codes SEQ ID NO：Amino acid sequence shown in 33；
The 2T5 gene codes SEQ ID NO：Amino acid sequence shown in 34.
7. biological synthesis gene cluster according to claim 5, it is characterised in that along 5 ' -3 ' ends of gene, the 2T2 Gene, the 2T1 genes, the 2G8 genes, the 2G7 genes, the 2G6 genes, the 2R4 genes, the 2R3 genes, It is the 2P1 genes, the 2A8 genes, the 2A11 genes, the 2A9 genes, the 2P2 genes, the 2I genes, described 2O genes, the 2A10 genes, the 2E genes, the 2A7 genes, the 2G5 genes, the 2G1 genes, the 2G2 bases Cause, the 2G3 genes, the 2G4 genes, the 2M genes, the 2A6 genes, the 2A5 genes, the 2A4 genes, institute State 2A3 genes, the 2A2 genes, the 2A1 genes, the 2R2 genes, the 2R1 genes, the 2T5 genes, the 2T4 Gene and the 2T3 genes are sequentially connected in series.
8. the biological synthesis gene cluster according to claim any one of 1-7, it is characterised in that the biological synthesis gene cluster With SEQ ID NO：Nucleotide sequence shown in 35.
9. a kind of microorganism, it is characterised in that the microorganism has the biosynthesis gene described in claim any one of 1-8 Cluster.
10. microorganism according to claim 9, it is characterised in that the microorganism is streptomycete.
A kind of 11. methods of prepare compound, it is characterised in that including：
Fermentation process is carried out using the microorganism described in claim 9 or 10, so as to the microorganism after being fermented；
Microorganism after the fermentation is carried out into broken wall treatment, to obtain the solution containing the compound；And
The compound is purified from the solution containing the compound, to obtain the sterling of the compound.
12. methods according to claim 11, it is characterised in that the time of the fermentation process is 7-8 days.
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