CN106889539A - A kind of method that utilization aqueous extraction-alcohol precipitation technology extracts honeycomb element from honeycomb - Google Patents
A kind of method that utilization aqueous extraction-alcohol precipitation technology extracts honeycomb element from honeycomb Download PDFInfo
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- CN106889539A CN106889539A CN201710098716.3A CN201710098716A CN106889539A CN 106889539 A CN106889539 A CN 106889539A CN 201710098716 A CN201710098716 A CN 201710098716A CN 106889539 A CN106889539 A CN 106889539A
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Abstract
The invention discloses a kind of method that utilization aqueous extraction-alcohol precipitation technology extracts honeycomb element from honeycomb, belong to honeycomb element preparation field, it is desirable to provide process is simple, the honeycomb element extracting method of low cost, step are as follows：Step one:Pulverization process is carried out to honeycomb；Step 2：Add water and boil, filter, decocting liquid is obtained, adjust decocting liquid concentration, just cream is obtained, and at 20 DEG C, precipitate more than 12h, remove sediment；Step 3:Ethanol, impurity screening are added in filtrate to step B；Step 4:Ethanol is removed by rotary evaporation, honeycomb element semifinished product is obtained after concentration.Step 5：Follow-up treatment is carried out to honeycomb element semifinished product.
Description
Technical field
It is that one kind utilizes aqueous extraction-alcohol precipitation technology from honeycomb specifically the present invention relates to a kind of extracting method of honeycomb element
The method for extracting honeycomb element.
Background technology
Honeycomb is that honeybee breeds the place that offspring stores grain, and honeybee husks, propolis, beeswax, Bee Pollen are maintain in honeycomb
Etc. various particular matters, thus honeycomb element refines and forms, and main component has aliphatic acid, tannin, glucoside, protein, amino acid, ox
Sulfonic acid, enzyme and trace element etc., the unknown material of also many compositions.
Number of patent application：201410467247.4th, the patent of invention of a kind of entitled honeycomb element and its process of preparing
A kind of honeycomb element and its process of preparing are disclosed, with honeycomb extract solution dried object, honey, beeswax, honeybee afterbirth, propolis, honeybee
Pollen is made by constituting, by weight percentage, honeycomb extract solution dried object 1-3%, honey 80-85%, beeswax 1-3%, honeybee tire
Clothing 1-2%, propolis 3-5%, Bee Pollen 1-1.5%.
The usual preparation method of existing method is complicated, and effective component extraction rate in honeycomb is relatively low, the honeycomb element of preparation
Middle impurity is also more, and mouthfeel is not enough.
The content of the invention
It is an object of the invention to：For above-mentioned problem, there is provided one kind is carried using aqueous extraction-alcohol precipitation technology from honeycomb
The method for taking honeycomb element, step is as follows：
Step one:Pulverization process is carried out to honeycomb；
Step 2：Add water and boil, filter, decocting liquid is obtained, adjust decocting liquid concentration, just cream is obtained, and at 20 DEG C, precipitate 12h
More than, remove sediment；
Step 3:To adding ethanol, impurity screening in the filtrate of step 2；
Step 4:Ethanol is removed by rotary evaporation, honeycomb element semifinished product is obtained after concentration.
Step 5：Follow-up treatment is carried out to honeycomb element semifinished product.
In preparation, just cream concentration is excessive, then liquid viscosity is higher, and ethanol is difficult to be fully contacted with cream liquid, is produced
Raw precipitation easily wraps up liquid, causes the loss of active ingredient；First cream concentration is too low, then cream liquid measure is larger, need to expend a large amount of second
Alcohol, extends activity time, wastes time and energy.Therefore, select suitable first cream concentration extremely important to aqueous extraction-alcohol precipitation technology,
On the basis of experiment, the concentration of the first cream of present invention selection is 1:1-1:4.
Used as improvement, the concentration of the ethanol of described step three is 60%-95%.
Individual in the middle of the process for preparing, the concentration of ethanol can use conventional concentration, but if alcohol content is few,
The magazine in just cream can not be fully removed, ethanol can be wasted again if ethanol is more, increase old of extraction, so appropriate ethanol
Concentration is most important, and on the basis of many experiments, in detection on 87%, removal of impurity does not have very big change to the present invention,
And under 80%, with increasing for ethanol content, removal of impurity can be raised comparatively fast, so present invention selection ethanol is optimal
Concentration range is 80%-87%.
The time of decocting directly influences recovery rate, if the time passes section, effective component extraction rate is inadequate, preferably,
It is 2 h -20h that the time of decocting waits.
The clearance alcohol precipitation temperature that alcohol precipitation temperature directly influences impurity is 3 DEG C -10 DEG C, and quiescent time is 12 h -36h.
Gradation alcohol precipitation steps up concentration of alcohol with gradient incremental manner, be conducive to remove impurity, with reduce effectively into
The loss for dividing.As improvement, by the way of gradation plus alcohol, it is divided into 2-5 times.
Relative to it is existing extraction honeycomb element method, process is simple of the invention, cost it is relatively low and prepare honeycomb
The impurity content of element is few, and effective component extraction rate is high in honeycomb.
Specific embodiment
Specific embodiment 1：Pulverization process is carried out to honeycomb first, add water pan-fried 2h, filtered, decocting liquid is obtained, adjust decocting
Liquid concentration, is obtained just cream, just cream concentration 1:1, and at 5 DEG C, 12h is precipitated, remove sediment；Secondly, it is divided into 2 addition ethanol,
Equal per secondary amounts, ethanol content is 80%, while stirring, is placed on static 12 h, impurity screening under 3 DEG C of environment after stirring；Finally,
Ethanol is removed by rotary evaporation, honeycomb element semifinished product is obtained after concentration and follow-up treatment is carried out.
Specific embodiment 2：Pulverization process is carried out to honeycomb first, add water pan-fried 20h, filtered, decocting liquid is obtained, adjust decocting
Liquid concentration, is obtained just cream, just cream concentration 1:4, and at 15 DEG C, 24 h are precipitated, remove sediment；Secondly, it is divided into 3 addition second
Alcohol, equal per secondary amounts, ethanol content is 87%, while stirring, is placed on static 36h, impurity screening under 3 DEG C of environment after stirring；Most
Afterwards, ethanol is removed by rotary evaporation, honeycomb element semifinished product is obtained after concentration and follow-up treatment is carried out.
Specific embodiment 3：Pulverization process is carried out to honeycomb first, add water pan-fried 10h, filtered, decocting liquid is obtained, adjust decocting
Liquid concentration, is obtained just cream, just cream concentration 1:2, and at 15 DEG C, 12h is precipitated, remove sediment；Secondly, it is divided into 2 addition second
Alcohol, equal per secondary amounts, ethanol content is 87%, while stirring, is placed on static 12 h, impurity screening under 3 DEG C of environment after stirring；Most
Afterwards, ethanol is removed by rotary evaporation, honeycomb element semifinished product is obtained after concentration and follow-up treatment is carried out.
Specific embodiment 4：Pulverization process is carried out to honeycomb first, add water pan-fried 20h, filtered, decocting liquid is obtained, adjust decocting
Liquid concentration, is obtained just cream, just cream concentration 1：3, and at 5 DEG C, 12 h are precipitated, remove sediment；Secondly, it is divided into 2-5 addition second
Alcohol, equal per secondary amounts, ethanol content is 88%, while stirring, is placed on static 24h, impurity screening under 10 DEG C of environment after stirring；Most
Afterwards, ethanol is removed by rotary evaporation, honeycomb element semifinished product is obtained after concentration and follow-up treatment is carried out.
Specific embodiment 5：Pulverization process is carried out to honeycomb first, add water pan-fried 20h, filtered, decocting liquid is obtained, adjust decocting
Liquid concentration, is obtained just cream, just cream concentration 1：1, and at 10 DEG C, 12h-36 h are precipitated, remove sediment；Secondly, it is divided into 5 additions
Ethanol, equal per secondary amounts, ethanol content is 85%, while stirring, is placed on static 36h, impurity screening under 5 DEG C of environment after stirring；Most
Afterwards, ethanol is removed by rotary evaporation, honeycomb element semifinished product is obtained after concentration and follow-up treatment is carried out.
Claims (7)
1. a kind of method that utilization aqueous extraction-alcohol precipitation technology extracts honeycomb element from honeycomb, it is characterised in that step is as follows：
Step one:Pulverization process is carried out to honeycomb；
Step 2：Add water and boil, filter, decocting liquid is obtained, adjust decocting liquid concentration, just cream is obtained, and at 20 DEG C, precipitate 12h
More than, remove sediment；
Step 3:To adding ethanol, impurity screening in the filtrate of step 2；
Step 4:Ethanol is removed by rotary evaporation, honeycomb element semifinished product is obtained after concentration；
Step 5：Follow-up treatment is carried out to honeycomb element semifinished product.
2. the method that utilization aqueous extraction-alcohol precipitation technology according to claim 1 extracts honeycomb element from honeycomb, it is characterised in that institute
The concentration of the first cream of the step of stating two is 1:1-1:4.
3. the method that utilization aqueous extraction-alcohol precipitation technology according to claim 1 extracts honeycomb element from honeycomb, it is characterised in that institute
The concentration of the ethanol of the step of stating three is 60%-95%.
4. the method that utilization aqueous extraction-alcohol precipitation technology according to claim 3 extracts honeycomb element from honeycomb, it is characterised in that institute
The concentration of the ethanol stated is 80%-87%.
5. the method that utilization aqueous extraction-alcohol precipitation technology according to claim 1 extracts honeycomb element from honeycomb, it is characterised in that institute
The time for stating decocting in step 2 is 2 h -20h.
6. the method that utilization aqueous extraction-alcohol precipitation technology according to claim 1 extracts honeycomb element from honeycomb, it is characterised in that institute
Alcohol content is 40%-80% in stating step 3, and alcohol precipitation temperature is 3 DEG C -10 DEG C, and quiescent time is 12 h -36h.
7. the method that utilization aqueous extraction-alcohol precipitation technology according to claim 6 extracts honeycomb element from honeycomb, it is characterised in that adopt
With gradation plus the mode of alcohol, it is divided into 2-5 times.
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| CN201710098716.3A CN106889539A (en) | 2017-02-23 | 2017-02-23 | A kind of method that utilization aqueous extraction-alcohol precipitation technology extracts honeycomb element from honeycomb |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111789870A (en) * | 2020-08-11 | 2020-10-20 | 江西省农业科学院畜牧兽医研究所 | Method for extracting honeycomb flavone with response surface under assistance of ultrasonic waves, honeycomb flavone and application |
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| CN102423032A (en) * | 2011-10-14 | 2012-04-25 | 安徽省宿松县刘氏蜂产品有限责任公司 | Processing technology of honeycomb element |
| CN102511705A (en) * | 2011-12-05 | 2012-06-27 | 芜湖蜂联有限公司 | Honeycomb element cream and preparing method thereof |
| CN103230012A (en) * | 2013-05-08 | 2013-08-07 | 福建新之源生物制品有限公司 | Honeycomb essence buccal tablet and preparation process thereof |
| CN103766688A (en) * | 2013-06-29 | 2014-05-07 | 明光市昊昊蜂业有限公司 | Honeycomb element chewable tablets and preparation method thereof |
| CN104273411A (en) * | 2014-09-15 | 2015-01-14 | 安徽蜂献蜂业有限公司 | Honeycomb extract and preparation process method thereof |
| KR20160043167A (en) * | 2014-10-10 | 2016-04-21 | 대한민국(농촌진흥청장) | Method for seperating bee venom containing active amines and food composition thereof |
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2017
- 2017-02-23 CN CN201710098716.3A patent/CN106889539A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102423032A (en) * | 2011-10-14 | 2012-04-25 | 安徽省宿松县刘氏蜂产品有限责任公司 | Processing technology of honeycomb element |
| CN102511705A (en) * | 2011-12-05 | 2012-06-27 | 芜湖蜂联有限公司 | Honeycomb element cream and preparing method thereof |
| CN103230012A (en) * | 2013-05-08 | 2013-08-07 | 福建新之源生物制品有限公司 | Honeycomb essence buccal tablet and preparation process thereof |
| CN103766688A (en) * | 2013-06-29 | 2014-05-07 | 明光市昊昊蜂业有限公司 | Honeycomb element chewable tablets and preparation method thereof |
| CN104273411A (en) * | 2014-09-15 | 2015-01-14 | 安徽蜂献蜂业有限公司 | Honeycomb extract and preparation process method thereof |
| KR20160043167A (en) * | 2014-10-10 | 2016-04-21 | 대한민국(농촌진흥청장) | Method for seperating bee venom containing active amines and food composition thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111789870A (en) * | 2020-08-11 | 2020-10-20 | 江西省农业科学院畜牧兽医研究所 | Method for extracting honeycomb flavone with response surface under assistance of ultrasonic waves, honeycomb flavone and application |
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