CN106872408B - Plankton imaging detection device - Google Patents
Plankton imaging detection device Download PDFInfo
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- CN106872408B CN106872408B CN201710280533.3A CN201710280533A CN106872408B CN 106872408 B CN106872408 B CN 106872408B CN 201710280533 A CN201710280533 A CN 201710280533A CN 106872408 B CN106872408 B CN 106872408B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
- G01N21/45—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods
- G01N21/453—Holographic interferometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8405—Application to two-phase or mixed materials, e.g. gas dissolved in liquids
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a plankton imaging detection device. The device comprises: the device comprises a first laser, a second laser and a color camera, wherein light emitted by the first laser is divided into reference light and object light by a beam splitter prism, the reference light enters the color camera, the object light enters the color camera after passing through a plankton imaging detection area, and the reference light and the object light interfere with each other in the color camera; light emitted by the second laser passes through the plankton imaging detection area and then enters the color camera; the color of the light emitted by the first laser is different from that of the light emitted by the second laser, and the reference light, the object light and the light emitted by the second laser and passing through the plankton imaging detection area can be collected by the color camera in one exposure. The plankton imaging detection device provided by the invention can realize high-resolution in-situ imaging detection of plankton under the condition of turbid water quality.
Description
Technical Field
The invention relates to the field of imaging detection of plankton in water, in particular to an imaging detection device of plankton.
Background
Currently, plankton detection methods generally collect plankton by using a sampling net and carry the plankton back to a laboratory for detection, but some unavoidable damage to the plankton is generated. Water samples containing plankton are also brought back to a laboratory for detection by a water sample collector, but the sampling workload is particularly large. At present, a general underwater camera has no microscopic imaging function, so that the camera can only be used for imaging large-scale aquatic organisms. For plankton with the body size below 1mm, microscopic amplification is generally required to carry out imaging detection on the plankton, but the depth of field of a microscope is small, and the method is not suitable for carrying out imaging detection on plankton swimming in a 3-dimensional space of a water body.
The current technology is to use on-axis digital holography or off-axis digital holography to image and detect plankton. But the coaxial digital hologram is only suitable for imaging sparse objects on a transparent background, and cannot be suitable for the condition that the water quality is turbid. Off-axis digital holography can separate a reproduction image of an object from a conjugate image, but requires a larger distance between the object to be measured and the image sensor, and the numerical aperture of the system is smaller without adding an optical magnifying lens, resulting in lower imaging resolution, and the imaging field of view is smaller if an optical magnifying lens is added. Therefore, the prior art can not solve the problem of high-resolution in-situ imaging of plankton under the condition of turbid water quality.
Disclosure of Invention
The invention aims to provide a plankton imaging detection device which can realize high-resolution in-situ imaging detection of plankton under the condition of turbid water quality.
In order to achieve the above object, the present invention provides the following solutions:
an plankton imaging detection device, the device comprising: the device comprises a first laser, a second laser and a color camera, wherein light emitted by the first laser is divided into reference light and object light by a beam splitter prism, the reference light enters the color camera, the object light enters the color camera after passing through a plankton imaging detection area, and the reference light and the object light interfere with each other in the color camera; the light emitted by the second laser passes through the plankton imaging detection area and then enters the color camera; the color camera can collect the reference light, the object light and the light emitted by the second laser and passing through the plankton imaging detection area in one exposure.
Optionally, the plankton imaging detection device is externally wrapped with a sealing shell, and a first optical window and a second optical window are arranged on the sealing shell.
Optionally, the plankton imaging detection device further includes: the optical fiber system comprises a first optical fiber, a second optical fiber, a first collimating lens, a second collimating lens, a first beam splitter prism, a second beam splitter prism, a third beam splitter prism, a first reflecting mirror and a second reflecting mirror; the light emitted by the first laser is output through a first optical fiber, is changed into parallel light through the first collimating lens, is divided into two beams by the first beam splitting prism, wherein one beam is the reference light, the other beam is the object light, the reference light is reflected by the first reflecting mirror and then is reflected to an image sensor of the color camera through the second beam splitting prism, the object light is reflected by the third beam splitting prism and then enters the plankton imaging detection area outside the sealed shell through the first optical window, enters the sealed shell through the second optical window, and then is irradiated to the image sensor of the color camera through the second beam splitting prism to interfere with the reference light; the light emitted by the second laser is output through the second optical fiber, is changed into parallel light after being reflected by the second collimating lens, passes through the third beam splitting prism, passes through the first optical window and enters the plankton imaging detection area outside the sealing shell, passes through the second optical window and enters the sealing shell, and finally passes through the second beam splitting prism and irradiates onto the image sensor of the color camera, and the color camera can collect the reference light irradiated onto the color camera, the object light and the light emitted by the second laser and passing through the plankton imaging detection area at the same time in one exposure.
Optionally, the plankton imaging detection device further comprises a controller, wherein the controller is used for controlling the brightness of the first laser and the second laser and the action of the color camera.
Optionally, the plankton imaging detection device further comprises a battery power supply, wherein the battery power supply is used for supplying power to the first laser, the second laser and the color camera.
Optionally, the plankton imaging detection device further comprises a control monitor, and the control monitor is respectively connected with the controller and the color camera through cables.
Optionally, the control monitor comprises a monitor and a central processor, and the monitor is used for displaying the image acquired by the color camera and parameters of the color camera, the first laser and the second laser; the central processing unit is used for setting parameters of the first laser and the second laser, controlling the color camera to shoot a holographic picture or video through the controller, and completing imaging of plankton in the plankton imaging detection area according to the holographic picture or video.
Optionally, the control monitor is further provided with a charging interface and an image video deriving interface, the charging interface is used for charging a power battery, and the image video deriving interface is used for deriving pictures or videos shot by the color camera.
According to the specific embodiment provided by the invention, the invention discloses the following technical effects: the plankton imaging detection device provided by the invention adopts a dual-wavelength coaxial off-axis composite digital holographic technology to realize in-situ imaging detection of plankton in a water body, utilizes two laser light sources with different wavelengths to respectively form an off-axis digital hologram and a coaxial digital hologram, utilizes a color camera to realize simultaneous recording of the off-axis digital hologram and the coaxial digital hologram by single exposure (one-time exposure), and completes high-resolution imaging of plankton under the condition of more turbid water quality according to the simultaneously recorded off-axis digital hologram and the coaxial digital hologram.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions of the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of an imaging detection device for underwater plankton according to an embodiment of the present invention;
fig. 2 is a schematic diagram of a control monitor according to an embodiment of the invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention aims to provide a plankton imaging detection device which can realize high-resolution in-situ imaging detection of plankton under the condition of turbid water quality.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
Fig. 1 is a schematic structural diagram of an underwater plankton imaging detection device according to an embodiment of the present invention, as shown in fig. 1, the plankton imaging detection device includes: the light emitted by the first laser 101 is divided into reference light and object light by a beam splitter prism, the reference light enters the color camera 107, the object light enters the color camera 107 after passing through a plankton imaging detection area 110, and the reference light and the object light interfere with each other in the color camera; the light emitted by the second laser 112 passes through the plankton imaging detection area 110 and then enters the color camera 107; the color camera 107 can collect the reference light, the object light and the light emitted from the second laser 112 after passing through the plankton imaging detection area 111, which are irradiated to the color camera, at the same time in one exposure, when the color of the light emitted from the first laser 101 is different from the color of the light emitted from the second laser 112 (two of the three colors of red, green and blue). The plankton imaging detection device is externally wrapped with a sealing shell 116, and a first optical window 109 and a second optical window 111 are arranged on the sealing shell 116.
Specifically, the light emitted by the first laser 101 is output through the first optical fiber 102, then is changed into parallel light through the first collimating lens 103, and is then split into two beams by the first beam splitter prism 104, wherein one beam is the reference light, the other beam is the object light, the reference light is reflected by the first reflecting mirror 105 and then is reflected by the second beam splitter prism 106 to the image sensor of the color camera 107, the object light is reflected by the third beam splitter prism 108 and then enters the plankton imaging detection area 110 outside the sealed shell through the first optical window 109, then enters the sealed shell through the second optical window 111, and then is irradiated onto the image sensor of the color camera 107 through the second beam splitter prism 106, and mutually interferes with the reference light to form an off-axis digital hologram; the light emitted by the second laser 112 is output through the second optical fiber 113, is changed into parallel light through the second collimating lens 114, is reflected by the second reflecting mirror 115, passes through the third beam splitting prism 108, passes through the first optical window 109, enters the plankton imaging detection area 110 outside the sealed shell, passes through the second optical window 111, enters the sealed shell, and finally passes through the second beam splitting prism 106 to be irradiated onto the image sensor of the color camera 107, so as to form a coaxial digital hologram; the color camera 107 can collect the reference light, the object light, and the light emitted from the second laser 112 after passing through the plankton imaging detection area 110, which are irradiated to the color camera, at the same time in one exposure.
The plankton imaging detection apparatus further comprises a controller 117, the controller 117 being configured to control the brightness of the first laser 101 and the second laser 112 and the actions of the color camera 107, the actions comprising: such as controlling the exposure of the color camera 107, taking holograms, video, etc.
The plankton imaging detection device further comprises a battery power supply 118, wherein the battery power supply 118 is used for supplying power to the first laser 101, the second laser 112 and the color 107 camera.
The plankton imaging detection device further comprises a control monitor 119, wherein the control monitor 119 is respectively connected with the controller 117 and the color camera 107 through a cable 120.
Fig. 2 is a schematic diagram of a control monitor according to an embodiment of the present invention, as shown in fig. 2, the control monitor 119 includes a monitor 201 and a central processor, where the monitor 201 is configured to display an image acquired by the color camera 107 and parameters of the color camera 107, the first laser 101, and the second laser 112; the central processing unit is used for setting parameters of the first laser 101 and the second laser 112, controlling the color camera 107 to shoot a hologram or video through the controller 117, and completing imaging of plankton in the plankton imaging detection area 110 according to the hologram or video.
The control monitor 119 is also provided with an adjustment keypad 203 which can be used for setting parameters of the color camera 107 and the laser. The picture recording key 202 and the video recording key 204 are used to operate to take a hologram or a hologram video. The control monitor 119 is further provided with a charging interface for charging the power battery 118 and an image video export interface for exporting pictures or videos taken by the color camera 107.
When the imaging detection device is used, the underwater plankton imaging detection device is put into water by using the bearing cable, a digital hologram or a digital holographic video is recorded by controlling the monitor to operate, the hologram or the video is reproduced by using a double-wavelength coaxial off-axis composite digital holographic reproduction algorithm after the computer is led into the computer, the reproduction distance in the program can be automatically changed from Z1 to Z2 (Z1 is the distance between the color camera image sensor and the second optical window, and Z2 is the distance between the color camera image sensor and the first optical window), and imaging detection of plankton in the imaging detection area can be realized.
The invention is based on a dual-wavelength on-axis off-axis composite digital hologram technology, adopts low-precision phase distribution phi off of Object light waves in an image sensor plane (Recordingplane) reconstructed from an off-axis digital hologram (Ioff) as initial phase distribution of phase recovery iterative reconstruction of an on-axis digital hologram (Iin), and adds low-precision phase distribution phi 0-off of the Object light waves reconstructed from the off-axis digital hologram (Ioff) into an Object to be detected (Object) plane as constraint conditions of phase recovery iterative reconstruction of the on-axis digital hologram (Iin). Therefore, the invention is suitable for imaging detection of plankton with body size in the range of 10 micrometers to 1 millimeter in water under the condition that the water quality is relatively turbid or the concentration of plankton (such as algae) is relatively high. Moreover, the invention has the characteristics of higher resolution and larger view field and high detection efficiency. The resolution of the imaging field is about equal to the pixel size of the image sensor of the color camera, and the imaging field is equal to the photosensitive area of the image sensor. The volume of water which can be detected by shooting one hologram is V=S.d, wherein S is the photosensitive area of the image sensor, and d is the distance from the first optical window to the second optical window.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other. For the system disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant points refer to the description of the method section.
The principles and embodiments of the present invention have been described herein with reference to specific examples, the description of which is intended only to assist in understanding the methods of the present invention and the core ideas thereof; also, it is within the scope of the present invention to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the invention.
Claims (6)
1. An plankton imaging detection device, the device comprising: the device comprises a first laser, a second laser and a color camera, wherein light emitted by the first laser is divided into reference light and object light by a beam splitter prism, the reference light enters the color camera, the object light enters the color camera after passing through a plankton imaging detection area, and the reference light and the object light interfere with each other in the color camera; the light emitted by the second laser passes through the plankton imaging detection area and then enters the color camera; the color camera can collect the reference light, the object light and the light emitted by the second laser and passing through the plankton imaging detection area in one exposure;
the apparatus further comprises: a controller for controlling brightness of the first and second lasers and an action of the color camera; and the battery power supply is used for supplying power to the first laser, the second laser and the color camera.
2. The plankton imaging detection apparatus according to claim 1, wherein a sealed housing is wrapped outside the plankton imaging detection apparatus, and a first optical window and a second optical window are provided on the sealed housing.
3. The plankton imaging detection apparatus according to claim 2, wherein the plankton imaging detection apparatus further comprises: the optical fiber system comprises a first optical fiber, a second optical fiber, a first collimating lens, a second collimating lens, a first beam splitter prism, a second beam splitter prism, a third beam splitter prism, a first reflecting mirror and a second reflecting mirror; the light emitted by the first laser is output through a first optical fiber, is changed into parallel light through the first collimating lens, is divided into two beams by the first beam splitting prism, wherein one beam is the reference light, the other beam is the object light, the reference light is reflected by the first reflecting mirror and then is reflected to an image sensor of the color camera through the second beam splitting prism, the object light is reflected by the third beam splitting prism and then enters the plankton imaging detection area outside the sealed shell through the first optical window, enters the sealed shell through the second optical window, and then is irradiated to the image sensor of the color camera through the second beam splitting prism to interfere with the reference light; the light emitted by the second laser is output through the second optical fiber, is changed into parallel light after being reflected by the second collimating lens, passes through the third beam splitting prism, passes through the first optical window and enters the plankton imaging detection area outside the sealing shell, passes through the second optical window and enters the sealing shell, and finally passes through the second beam splitting prism and irradiates onto the image sensor of the color camera, and the color camera can collect the reference light irradiated onto the color camera, the object light and the light emitted by the second laser and passing through the plankton imaging detection area at the same time in one exposure.
4. The plankton imaging detection apparatus according to claim 1, further comprising a control monitor connected to the controller and the color camera via cables, respectively.
5. The plankton imaging detection apparatus according to claim 4, wherein the control monitor includes a monitor for displaying an image acquired by the color camera and parameters of the color camera, the first laser, and the second laser, and a central processor; the central processing unit is used for setting parameters of the first laser and the second laser, controlling the color camera to shoot a holographic picture or video through the controller, and completing imaging of plankton in the plankton imaging detection area according to the holographic picture or video.
6. The plankton imaging detection apparatus according to claim 4, wherein the control monitor is further provided with a charging interface for charging a power supply battery and an image video derivation interface for deriving a picture or video taken by the color camera.
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