CN109443535A - A kind of underwater microspectrum imaging device and method based on fast illuminated spectrum camera - Google Patents
A kind of underwater microspectrum imaging device and method based on fast illuminated spectrum camera Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/2823—Imaging spectrometer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
- G01J2003/102—Plural sources
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Abstract
The invention discloses a kind of underwater microspectrum imaging devices and method based on fast illuminated spectrum camera, which includes lighting module, imaging unit, microcontroller, sealed compartment, microcomputer, power module, seal case, bracket, human-computer interaction module;Wherein, the imaging unit includes fast illuminated spectrum camera, extension, the liquid lens, imaging microscope group for being sequentially connected and being arranged in same optical axis, the lighting module, imaging unit, microcontroller are mounted in sealed compartment internal stent, and the liquid lens and lighting module are connected with microcontroller;The microcomputer, power module, human-computer interaction module are mounted in seal case;The fast illuminated spectrum camera, microcontroller, human-computer interaction module are connected with microcomputer;Structure of the invention rationally, it is compact, easy to operate, can be optional with multiple-working mode by being observed operation under diver under water dynamic environment.
Description
Technical field
The present invention relates to a kind of microspectrum technical field of imaging more particularly to a kind of water based on fast illuminated spectrum camera
Lower microspectrum imaging device and method.
Background technique
Optical microscopy can be by the unresolvable small items amplification imaging of human eye, to extract fine structure information, quilt
It is widely used in the fields such as modern life science, drug medicine, test sensitivity.Spectral imaging technology ties up Spatial Dimension and spectrum
Degree combines, and can obtain intuitive, abundant, accurate optical characteristic of substance, leads in satellite remote sensing, material classification, military affairs etc.
Domain is widely used.Microspectrum imaging technique organically combines micro-imaging technique and spectral imaging technology one
It rises, becomes a kind of novel optical imaging technique.Microspectrum imager based on this technical research is mostly to push away the type of sweeping, that is, utilizes rib
Mirror light splitting or linear gradient filter array obtain the spectral information of subject, and imaging mode is pushed away for line and swept, then carries out image
Splicing;Or sweep type, such as divided based on rotation optical filter, acousto-optic tunable filter or liquid crystal tunable optical filter
Light acquires spectral cube data by spectral band sequence scanning.
However, correlative study and application mainly still concentrate on land, and for studying under water and using relatively fewer.With
The mankind deepen continuously to ocean research, people begin to focus on occur in the marine ecologies such as seaweed, sea grass bed, coral reef it is micro-
Meter level scale biological activity, to study its influence to marine ecology health status and long-term dynamics evolutionary process, such as coral
Albefaction, coral polyp growth, substrate structure etc., therefore, microspectrum imaging technique has wide application demand under water.The U.S.
Patent (patent publication No.: 2011/0205536 A1 of US), which proposes one kind, can be loaded into underwater hiding-machine bottom, for observing
The high light spectrum image-forming equipment of object in water, the device are commonly used to carry out water biological species, the underwater environment etc. in underwater environment
Imaging, but it is not directed to microscopic fields.
Summary of the invention
It is multiple that environment is complicated and changeable under water, physical and chemical biological activity intercouples, water body is decayed and scattered to optical transport etc.
Under interference, how to solve small, micron order depth of field of conventional micro-imaging operating distance etc. limitation, to underwater micron order scale activity into
Long-term, in situ, the glitch-free dynamic observation of row is still that there are many technological difficulties, such as fine focusing, indirect illumination, farm labourer
Make distance, laid without invasive.Aiming at the shortcomings in the prior art, the present invention provides a kind of based on fast illuminated spectrum camera
Underwater microspectrum imaging device and method.
In order to solve the above-mentioned technical problem, the present invention is achieved by the following technical solutions: one kind being based on fast illuminated light
Compose the underwater microspectrum imaging device of camera, including lighting module, imaging unit, microcontroller, sealed compartment, miniature calculating
Mechanical, electrical source module, seal case, bracket, human-computer interaction module;Wherein, it is aobvious to carry out micron order to object for the imaging unit
Micro- light spectrum image-forming, it include be sequentially connected and be arranged in fast illuminated spectrum camera in same optical axis, extension, liquid lens,
Microscope group is imaged, the lighting module illuminates object;The lighting module, imaging unit, microcontroller are mounted in sealed compartment
On portion's bracket, the liquid lens and lighting module are connected with microcontroller, by microcontroller control lighting module open and
It closes;The microcomputer, power module, human-computer interaction module are mounted in seal case, are shown under power module water supply
Micro- optical spectrum imaging device provides operating voltage;The fast illuminated spectrum camera, microcontroller, human-computer interaction module are and microcomputer
Calculation machine is connected;On the sealed compartment and seal case mounting bracket.
Further, the imaging microscope group includes Zoom optic lens or long reach image-forming objective lens, and the optics becomes
The optical magnification of zoom lens or long reach object lens is greater than 2 times;Microcontroller controls liquid lens by voltage signal
Lens shape, response speed is Millisecond, change imaging unit equivalent focal length, be greater than 5cm micro-imaging operating distance
Lower realization focusing, and there is grade adjustable working distance range.
Further, the Polaroid spectrum picture that can be obtained target complete wave band of fast illuminated spectrum camera, can
For acquiring Frame projection spectrum picture or spectrum video.
Further, the lighting module includes ring support and LED combination array;The ring support and imaging microscope group
Coaxially;The LED combination array includes multiple LED array units, and each LED array unit includes each and wave band pair interested
The narrow spectrum LED combination answered;The LED array unit in conjunction with snoot, installed by even circumferential by the LED array unit
In ring support;Microcontroller by open or close in LED array unit the narrow spectrum LED of a certain service band come
Change illumination spectrum.
Further, the drive end bearing bracket of the sealed compartment is provided with optical glass window, the imaging microscope group front end face close to
Optical glass;Underwater electrical connector is installed on the rear end cap of the sealed compartment, as real-time connection imaging end interface.
Further, 3 underwater electrical connectors are installed in the seal case side, respectively as real-time connection manipulation termination
Mouth, external power source interface, data output interface;The real-time connection manipulation end interface is connected with imaging end interface is connect in real time;
The external power source interface charges for providing external power supply to power module;The data output interface is used for microcomputer
It is connect with outer computer, carries out data output preservation, program updates modification.
Further, the human-computer interaction module includes display screen and underwater key module;Display screen and underwater key mould
Block is connected with microcomputer, the underwater key module include auto-focusing control button, manual focus control button, from
Dynamic spectrum assignment key, Manual exposure control button, control button of taking pictures, video recording control button, light illumination mode control button.
It is a further object of the present invention to provide a kind of underwater microspectrum imaging methods based on fast illuminated spectrum camera, should
Method realizes that this method includes following step in the above-mentioned underwater microspectrum imaging device based on fast illuminated spectrum camera
It is rapid:
(1) sealed compartment and seal box are mounted on the bracket of adjustable-angle, imaging unit alignment target object, and close
Batten down optical glass and object keep the micro-imaging operating distance of 5cm or more;
(2) by clicking auto-focusing control button, microcomputer notice microcontroller is driven by scanning voltage signal
Dynamic liquid lens are constantly changing shape, to change the equivalent focal length of imaging unit, microcomputer calculates fast illuminated in real time
Spectrum camera acquires the clarity of image, clearest at image focus to be automatically found;
(3) by manual focus control button, the voltage signal that microcontroller is applied on liquid lens can be altered in steps,
To manually change imaging unit equivalent focal length, change focal position;
(4) by auto-exposure control key, microcomputer presses time for exposure initial value to fast illuminated spectrum camera automatically
The image of acquisition is calculated, and the time for exposure for being suitble to all working wave band is calculated;
(5) by Manual exposure control button, current exposure time can be manually adjusted;
(6) by control button of taking pictures, the fast illuminated spectrum picture of a width can be acquired, image had both included Spatial Dimension information,
It again include spectral Dimensions, therefore microcomputer is automatically extracted using Data Analysis Services algorithm and rebuilds spectral cube data;
(7) by video recording control button, the fast illuminated spectrum video in a period of time is acquired, then video both includes space dimension
Spend information, spectral Dimensions, and include time dimension, so as to observed object object time dimension activity change, while
It can use frames fusion algorithm continuous multiple frames in video permeate the spectrum picture of vertical frame dimension dynamic range;
(8) by light illumination mode control button, control switch different narrow spectrum LED can change illumination according to actual needs
The spatial distribution of module outgoing;
(9) if object three-dimensional surface height changes obviously, since the micron order depth of field limits, there was only focus position on image
The part set be clearly, remaining beyond the depth of field part be it is fuzzy, pass through double-click auto-focusing control button, miniature calculating
Machine controls microcontroller to scan back and forth the focal length of switching liquid lens, so that a series of image of different focal point positions is obtained,
By microcomputer (on frames fusion algorithm, obtain target surface different parts all clearly images.
Compared with prior art, the beneficial effects of the present invention are:
The present invention uses fast illuminated spectrum camera as image-forming block, can the Polaroid light for obtaining full service band data
Spectrogram picture avoids mechanical structure light splitting limitation, without putting down platform, it can be achieved that high dynamic high speed acquisition spectrum video by pushing away,
It is suitble to underwater bumpy weather;
The present invention is combined using the Zoom optic lens or long reach object lens of high magnification with liquid lens
Mode realizes the micron order micro-imaging of large free working distance, avoids conventional short distance micro-imaging mode under natural environment
The movable influence of object, and there is millimetre-sized operating distance adjustable extent, realize objective object different distance level
Shape library;
The present invention provides Uniform Illumination using ring-shaped LED array, and illumination light field focuses on imaging region, and illuminates light field
Spatial distribution it is adjustable, meet the specific demands such as microscopic imaging fluorescence, also can avoid specific band illumination to sensitive target object
It influences;
Compact of the present invention, conveniently lays, can in situ under water, long-term, showing without invasive acquisition object
Micro- spectrum picture or video, and there is man-machine interactive interface, can real time inspection imaging effect under water, and convenient grasped manually
Work is arranged, and meets a variety of actual demands.
Detailed description of the invention
It, below will be to required use in embodiment description in order to more clearly illustrate the technical solution of the embodiment of the present invention
Attached drawing be briefly described.Obviously, following attached drawings are only some embodiments of the present invention, for those skilled in the art
For, without creative efforts, other embodiments attached drawing can be obtained according to these attached drawings.
Fig. 1 is overall structure diagram of the invention;
Fig. 2 is schematic diagram of internal structure of the invention;
Fig. 3 is main hardware connection block diagram of the invention;
In figure, 1. auto-focusing control buttons, 2. manual focus control buttons, 3. auto-exposure control keys, 4. manually
Spectrum assignment key, 5. take pictures control button, 6. video recording control buttons, 7. light illumination mode control buttons, 8. seal cases, 9. electricity
Source module, 10. display screens, 11. microcomputers, 12. end interfaces of connection manipulation in real time, 13. external power source interfaces, 14. data
Output interface, 15. end interfaces of connection imaging in real time, 16. sealed compartment rear end caps, 17. sealed compartment cylinders, 18. sealed compartment front ends
Lid, 19. microcontrollers, 20. fast illuminated spectrum cameras, 21. extensions, 22. liquid lens, 23. screw thread adapter rings, 24. imagings
Microscope group, 25. lighting modules, 26. first support plates, 27. second support plates, 28. third support plates, 29. the 4th support plates, 30.
5th support plate.
Specific embodiment
The embodiment of the present invention is described in further detail below with reference to attached drawing.
As shown in Figure 1-3, the present invention provides a kind of underwater microspectrum imaging device based on fast illuminated spectrum camera, packet
Include lighting module 25, imaging unit, microcontroller 19, cylinder-shaped sealed compartment, microcomputer 11, underwater key module, display
Screen 10, power module 9, seal case 8, bracket etc.;Wherein, the imaging unit includes being sequentially connected and being arranged in same optical axis
On fast illuminated spectrum camera 20, extension 21, liquid lens 22, imaging microscope group 24, it is unified different to pass through screw thread adapter ring 23
The connecting interface of size, the imaging unit carry out microspectrum imaging to object under longer operating distance;The photograph
Bright 25 uniform illumination object of module;The lighting module 25, imaging unit, microcontroller 19 are mounted in cylinder-shaped sealed compartment
On portion's bracket;The microcomputer 11, display screen 10, power module 9 are mounted on inside seal case 8, the display screen 10,
Underwater key module is connect with microcomputer 11;The cylindrical shape sealed compartment and seal case is mountable on tripod or pacifies
On the bracket for the design of specific underwater environment.
The imaging microscope group 24 includes Zoom optic lens or long reach image-forming objective lens, and front end can add increment
Mirror, it is lower under the premise of operating distance is greater than 5cm to realize that object for the first time amplify by imaging;The Zoom optic lens or farm labourer
The optical magnification for making range Imaging object lens is greater than 2 times, and Zoom optic lens have the optical magnification of multi-gear adjustable, long
Operating distance image-forming objective lens then need to change optical magnification by replacement different model;The liquid lens 22 and micro-control
Device 19 processed is connected, and microcontroller 19 controls lens shape by voltage signal, is combined with imaging microscope group 24, is appointed in a certain range
Meaning changes imaging unit equivalent focal length, and response speed is Millisecond, the realization pair under the micro-imaging operating distance greater than 5cm
Coke, and there is grade adjustable working distance range;The extension 21 has simultaneously for guaranteeing that focus falls in imaging plane
Certain optical magnification;The fast illuminated spectrum camera 20 is in imaging sensor process directly by wave interested
The narrow band filter of section is integrated into pixel unit surface, and optical filter is distributed by mosaic array, Polaroid to can be obtained entirely
The spectrum picture of portion's target wave band;The fast illuminated spectrum camera 20 is connected with microcomputer 11, is controlled by microcomputer 11
It makes fast illuminated spectrum camera 20 and carries out data acquisition, data transmission, microcomputer 11 can Operational Data Analysis Processing Algorithm general
The Spatial Dimension of fast illuminated spectral image data is separated with spectral Dimensions, rebuilds spectral cube data.
The lighting module 25 includes ring support and LED combination array;The ring support can pass through at least 3 pieces of radial directions
Screw and imaging 24 outer surface of microscope group connect, and realize coaxial;The LED combination array includes multiple LED array units,
Each LED array unit is formed by each narrow spectrum LED combination corresponding with wave band interested;The LED array unit
In conjunction with snoot, so that beam angle is shunk, emergent light energy accumulating;The LED array unit is mounted on by even circumferential
In ring support, and to imaging 24 inclined of microscope group, LED illumination light field is focused in imaging region;The lighting module
25 are connected with microcontroller 19, and microcontroller 19, which can be uniformly controlled, opens or closes a certain operation wavelength in LED array unit
Narrow spectrum LED.
The cylindrical shape sealed compartment is made of metallic cylinder 17, drive end bearing bracket 18, rear end cap 16, internal stent etc., by more
Axially and radially O-ring realizes static seal in road;The internal stent includes the first support plate 26, the second support plate 27, third branch
Fagging 28, the 4th support plate 29, the 5th support plate 30;First support plate 26 is affixed by screw with rear end cap 16;It is described
Second support plate 27 is affixed by copper post or stud with the first support plate 26, has multiple holes for cloth in second support plate 27
Line;The third support plate 28 and the second support plate 27 are affixed by copper post or stud, the third support plate 28 simultaneously with photograph
The ring support of bright module 25 is affixed;4th support plate 29 is installed between the second support plate 27 and the first support plate 26,
For installing fixed fast illuminated spectrum camera 20, extension 21;5th support plate 30 is installed on the second support plate 27 and
Between one support plate 26, for installing fixed microcontroller 19;The drive end bearing bracket 18 is provided with optical glass window, makes exterior light
Line can enter imaging unit, and 24 front end face of imaging microscope group is close to optical glass;Watertight is installed to connect on the rear end cap 16
End interface 15 is imaged as connection in real time in plug-in unit;The seal case 8 is made of metal cavity, glass window, upper end cover etc., is led to
It crosses seal washer and realizes static seal, 3 underwater electrical connectors are installed in metal cavity side, respectively as real-time connection manipulation end interface
12, external power source interface 13, data output interface 14;The external power source interface 13 is filled for external power supply to power module 9
Electricity, the power module 9 is for being the power supply such as microcomputer 11, microcontroller 19, lighting module 25 during underwater operation;
The data output interface 14 is connect for microcomputer 11 with outer computer, carries out data output preservation, program updates
Modification;The real-time connection manipulation end interface 12 is connected with imaging end interface 15 is connect in real time, for meeting fast illuminated spectrum phase
Machine 20, microcontroller 19 are connected with microcomputer 11 and a variety of demands such as electrical transfer.
The microcomputer 11 is for device manipulation, data transmission, processing, analysis and storage;The fast illuminated spectrum
Camera 20, microcontroller 19, display screen 10, underwater key module are connected with microcomputer 11;The liquid lens 22 and photograph
Bright module 25 is connected with microcontroller 19;The display screen 10 and underwater key module form human-computer interaction module;The display
Screen 10 is interacted for microspectrum imaging real-time display with key controlled response;The underwater key module includes auto-focusing
Control button 1, manual focus control button 2, auto-exposure control key 3, Manual exposure control button 4, control button of taking pictures
5, video recording control button 6, light illumination mode control button 7, key have single keys presses operation, rapid double tap button operation different
Response is to realize different manipulation demands.
Fast illuminated spectrum camera can use the production of Ximea company XISPEC-KIT-SM4X4-VIS model in the present embodiment
Product, but be not limited to from;Zoom optic lens can be using the product of the MVL6X12Z model of Thorlabs company, but is not limited to
From;Liquid lens can use Optotune company EL-16-40-TC-VIS model product, but be not limited to from;Miniature calculating
Machine can use COMMELL company LP-175 model product, but be not limited to from.
As shown in figure 3, the underwater microspectrum imaging method based on fast illuminated spectrum camera, comprising the following steps:
(1) underwater microspectrum imaging device is mounted on the bracket of adjustable-angle, imaging unit alignment target object,
And cylinder-shaped sealed compartment optical glass and object keep the micro-imaging operating distance of 5cm or more;
(2) by clicking auto-focusing control button 1, microcomputer 11 notifies microcontroller 19 to believe by scanning voltage
Number driving liquid lens 22 constantly quickly change shape, to change the equivalent focal length of imaging unit, microcomputer 11 is real
When calculate the clarity that fast illuminated spectrum camera 20 acquires image, it is clearest at image focus to be automatically found;
(3) by manual focus control button 2, the telecommunications that microcontroller 19 is applied on liquid lens 22 can be altered in steps
Number, to manually change imaging unit equivalent focal length, change focal position;
(4) by auto-exposure control key 3, microcomputer 11 presses the time for exposure to fast illuminated spectrum camera 20 automatically
The image of initial value acquisition is calculated, and the time for exposure of suitable all working wave band is calculated;
(5) by Manual exposure control button 4, current exposure time can be manually adjusted;
(6) by control button 5 of taking pictures, the fast illuminated spectrum picture of a width can be acquired, image had both included Spatial Dimension information,
It again include spectral Dimensions, therefore microcomputer 11 can use Data Analysis Services algorithm and automatically extract reconstruction spectral cube number
According to;
(7) by video recording control button 6, the fast illuminated spectrum video in a period of time can be acquired, then video both includes sky
Between dimensional information, spectral Dimensions, and include time dimension, so as to observed object object time dimension activity change, together
When also can use frames fusion algorithm continuous multiple frames in video permeate the spectrum picture of vertical frame dimension dynamic range;
(8) by light illumination mode control button 7, controllable switch different narrow spectrum LED can change photograph according to actual needs
The spatial distribution that bright module 25 is emitted;
(9) if object three-dimensional surface height changes obviously, since the depth of field limits, there was only the portion of focal position on image
Divide is that clearly, remaining part beyond the depth of field is fuzzy, therefore by double-clicking auto-focusing control button 1, miniature calculating
Machine 11 is controlled microcontroller and is switched fast the focal length of liquid lens 22 back and forth with certain frequency, to obtain a series of differences
The image of focal position obtains target surface different parts all by the frames fusion algorithm on microcomputer 11
Clearly image.
The above, the only present invention preferably specific implementation citing, it is clear that described embodiment is only the present invention one
Section Example, instead of all the embodiments.Based on the embodiment of the present invention, those skilled in the art are not doing
Every other embodiment obtained under the premise of creative work out, belongs to protection scope of the present invention.It is all in the present invention
Within spirit and principle, any modification, equivalent replacement, improvement and so on are covered by the protection scope of the present invention,
The scope of protection of the invention shall be subject to the scope of protection specified in the patent claim.
Claims (8)
1. a kind of underwater microspectrum imaging device based on fast illuminated spectrum camera, which is characterized in that including lighting module
(25), imaging unit, microcontroller (19), sealed compartment, microcomputer (11), power module (9), seal case (8), branch
Frame, human-computer interaction module etc.;Wherein, the imaging unit to object carry out microspectrum imaging, it include be sequentially connected and
It is arranged in fast illuminated spectrum camera (20) in same optical axis, extension (21), liquid lens (22), imaging microscope group (24), institute
It states lighting module (25) and illuminates object;The lighting module (25), imaging unit, microcontroller (19) are mounted on sealed compartment
In internal stent, the liquid lens (22) and lighting module (25) are connected with microcontroller (19), by microcontroller (19)
Control the on and off of lighting module (25);The microcomputer (11), power module (9), human-computer interaction module are mounted on close
In joint sealing body (8), microspectrum imaging device provides operating voltage under power module (9) water supply;The fast illuminated spectrum
Camera (20), microcontroller (19), human-computer interaction module are connected with microcomputer (11);The sealed compartment and seal case
In mounting bracket.
2. the underwater microspectrum imaging device according to claim 1 based on fast illuminated spectrum camera, which is characterized in that
The imaging microscope group (24) includes Zoom optic lens or long reach image-forming objective lens, the Zoom optic lens or long work
Optical magnification apart from object lens is greater than 2 times;The lens that microcontroller (19) passes through voltage signal control liquid lens (22)
Shape, response speed are Millisecond, change imaging unit equivalent focal length, are realized under the micro-imaging operating distance greater than 5cm
Focusing, and there is grade adjustable working distance range.
3. the underwater microspectrum imaging device according to claim 1 based on fast illuminated spectrum camera, which is characterized in that
The fast Polaroid spectrum picture that can be obtained target complete wave band of illuminated spectrum camera (20), can be used for acquiring Frame projection
Spectrum picture or spectrum video.
4. the underwater microspectrum imaging device according to claim 1 based on fast illuminated spectrum camera, which is characterized in that
The lighting module (25) includes ring support and LED combination array;The ring support and imaging microscope group (24) are coaxial;It is described
LED combination array includes multiple LED array units, and each LED array unit includes each narrow light corresponding with wave band interested
Compose LED combination;For the LED array unit in conjunction with snoot, the LED array unit is mounted on annular branch by even circumferential
In frame;Microcontroller changes illumination light by opening or closing the narrow spectrum LED of a certain service band in LED array unit
Spectrum.
5. the underwater microspectrum imaging device according to claim 1 based on fast illuminated spectrum camera, which is characterized in that
The drive end bearing bracket (18) of the sealed compartment is provided with optical glass window, and imaging microscope group (24) front end face is close to optical glass;
Underwater electrical connector is installed on the rear end cap (16) of the sealed compartment, as real-time connection imaging end interface (15).
6. the underwater microspectrum imaging device according to claim 5 based on fast illuminated spectrum camera, which is characterized in that
3 underwater electrical connectors are installed in seal case (8) side, respectively as real-time connection manipulation end interface (12), external power supply
Interface (13), data output interface (14);Real-time connection manipulation end interface (12) connect imaging end interface (15) with real-time
It is connected;The external power source interface (13) is charged for providing external power supply to power module (9);The data output interface
(14) it is connect for microcomputer (11) with outer computer, carries out data output preservation, program updates modification.
7. the underwater microspectrum imaging device according to claim 1 based on fast illuminated spectrum camera, which is characterized in that
The human-computer interaction module includes display screen (10) and underwater key module;Display screen (10) and underwater key module with it is miniature
Computer (11) be connected, the underwater key module include auto-focusing control button (1), manual focus control button (2), from
Dynamic spectrum assignment key (3), Manual exposure control button (4), control button of taking pictures (5), video recording control button (6), illumination mould
Formula control button (7).
8. a kind of underwater microspectrum imaging method based on fast illuminated spectrum camera, which is characterized in that this method is wanted in right
It is realized in underwater microspectrum imaging device described in asking 7 based on fast illuminated spectrum camera, method includes the following steps:
(1) sealed compartment and seal box are mounted on the bracket of adjustable-angle, imaging unit alignment target object, and sealed compartment
Optical glass and object keep the micro-imaging operating distance of 5cm or more;
(2) by clicking auto-focusing control button (1), microcomputer (11) notice microcontroller (19) passes through scanning voltage
Signal driving liquid lens (22) is constantly changing shape, thus change the equivalent focal length of imaging unit, microcomputer (11)
The clarity of fast illuminated spectrum camera (20) acquisition image is calculated in real time, it is clearest at image focus to be automatically found;
(3) by manual focus control button (2), the electricity that microcontroller (19) is applied on liquid lens (22) can be altered in steps
Signal is pressed to change focal position to manually change imaging unit equivalent focal length;
(4) by auto-exposure control key (3), when microcomputer (11) is automatically to fast illuminated spectrum camera (20) by exposure
Between initial value acquire image calculated, calculate be suitble to all working wave band time for exposure;
(5) by Manual exposure control button (4), current exposure time can be manually adjusted;
(6) by control button of taking pictures (5), the fast illuminated spectrum picture of a width can be acquired, image had not only included Spatial Dimension information, but also
Comprising spectral Dimensions, therefore microcomputer (11) is automatically extracted using Data Analysis Services algorithm and rebuilds spectral cube data;
(7) by video recording control button (6), the fast illuminated spectrum video in a period of time is acquired, then video both includes space dimension
Spend information, spectral Dimensions, and include time dimension, so as to observed object object time dimension activity change, while
It can use frames fusion algorithm continuous multiple frames in video permeate the spectrum picture of vertical frame dimension dynamic range;
(8) by light illumination mode control button (7), control switch different narrow spectrum LED can change illumination mould according to actual needs
The spatial distribution of block (25) outgoing;
(9) if object three-dimensional surface height changes obviously, since the micron order depth of field limits, there was only focal position on image
Part be clearly, remaining beyond the depth of field part be it is fuzzy, pass through double-click auto-focusing control button (1), miniature calculating
Machine (11) controls microcontroller to scan back and forth the focal length of switching liquid lens (22), to obtain a series of different focal point positions
Image target surface different parts are obtained all clearly by the frames fusion algorithm on microcomputer (11)
Image.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109905585A (en) * | 2019-04-02 | 2019-06-18 | 浙江大学 | A kind of rotary-type underwater polarization camera |
CN109959452A (en) * | 2019-04-02 | 2019-07-02 | 浙江大学 | A kind of underwater fluorescence spectrum imager of pull-type and imaging method |
CN114442302A (en) * | 2020-11-03 | 2022-05-06 | 上海奕太智能科技有限公司 | Underwater microscope based on deformable lens and control method |
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Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102680100A (en) * | 2012-06-12 | 2012-09-19 | 青岛市光电工程技术研究院 | Portable type trace detection spectral imager |
CN103017901A (en) * | 2012-11-21 | 2013-04-03 | 南京邮电大学 | Spectral measurement method and micro spectrograph based on varifocus lens |
CN103411934A (en) * | 2013-07-18 | 2013-11-27 | 江苏大学 | Fluorescence microscopy hyperspectrum imaging system |
CN103940819A (en) * | 2014-04-23 | 2014-07-23 | 浙江工业大学之江学院 | Image acquisition processing device and method for surfaces of medium yarns of fabric under microscale |
CN103955051A (en) * | 2014-05-12 | 2014-07-30 | 广州博隆兴中信息科技有限公司 | Viable-tissue-oriented real-time automatic focusing microscopy imaging device and method |
CN205754956U (en) * | 2016-05-23 | 2016-11-30 | 浙江大学 | One spectrum adjustable illumination device under water |
CN106768333A (en) * | 2016-12-29 | 2017-05-31 | 中国科学院西安光学精密机械研究所 | Highly sensitive optical spectrum imaging device and imaging method under water |
CN107957295A (en) * | 2017-12-27 | 2018-04-24 | 中国科学院西安光学精密机械研究所 | A kind of snapshot-type high-space resolution spectrum imaging system for ocean undersea detection |
CN108225562A (en) * | 2016-12-13 | 2018-06-29 | 罗伯特·博世有限公司 | Fourier transform spectrometer, and the method for running Fourier transform spectrometer, |
CN209197900U (en) * | 2018-10-30 | 2019-08-02 | 浙江大学 | A kind of underwater microspectrum imaging device based on fast illuminated spectrum camera |
-
2018
- 2018-10-30 CN CN201811276566.1A patent/CN109443535A/en active Pending
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102680100A (en) * | 2012-06-12 | 2012-09-19 | 青岛市光电工程技术研究院 | Portable type trace detection spectral imager |
CN103017901A (en) * | 2012-11-21 | 2013-04-03 | 南京邮电大学 | Spectral measurement method and micro spectrograph based on varifocus lens |
CN103411934A (en) * | 2013-07-18 | 2013-11-27 | 江苏大学 | Fluorescence microscopy hyperspectrum imaging system |
CN103940819A (en) * | 2014-04-23 | 2014-07-23 | 浙江工业大学之江学院 | Image acquisition processing device and method for surfaces of medium yarns of fabric under microscale |
CN103955051A (en) * | 2014-05-12 | 2014-07-30 | 广州博隆兴中信息科技有限公司 | Viable-tissue-oriented real-time automatic focusing microscopy imaging device and method |
CN205754956U (en) * | 2016-05-23 | 2016-11-30 | 浙江大学 | One spectrum adjustable illumination device under water |
CN108225562A (en) * | 2016-12-13 | 2018-06-29 | 罗伯特·博世有限公司 | Fourier transform spectrometer, and the method for running Fourier transform spectrometer, |
CN106768333A (en) * | 2016-12-29 | 2017-05-31 | 中国科学院西安光学精密机械研究所 | Highly sensitive optical spectrum imaging device and imaging method under water |
CN107957295A (en) * | 2017-12-27 | 2018-04-24 | 中国科学院西安光学精密机械研究所 | A kind of snapshot-type high-space resolution spectrum imaging system for ocean undersea detection |
CN209197900U (en) * | 2018-10-30 | 2019-08-02 | 浙江大学 | A kind of underwater microspectrum imaging device based on fast illuminated spectrum camera |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109905585A (en) * | 2019-04-02 | 2019-06-18 | 浙江大学 | A kind of rotary-type underwater polarization camera |
CN109959452A (en) * | 2019-04-02 | 2019-07-02 | 浙江大学 | A kind of underwater fluorescence spectrum imager of pull-type and imaging method |
CN114442302A (en) * | 2020-11-03 | 2022-05-06 | 上海奕太智能科技有限公司 | Underwater microscope based on deformable lens and control method |
CN117589703A (en) * | 2023-11-29 | 2024-02-23 | 中国科学院武汉岩土力学研究所 | Penetration type hyperspectral imaging detection device and method |
CN117589703B (en) * | 2023-11-29 | 2024-05-10 | 中国科学院武汉岩土力学研究所 | Penetration type hyperspectral imaging detection device and method |
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