CN106857682A - A kind of biological control method of dense planting jujube garden black spot - Google Patents

A kind of biological control method of dense planting jujube garden black spot Download PDF

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Publication number
CN106857682A
CN106857682A CN201710061423.8A CN201710061423A CN106857682A CN 106857682 A CN106857682 A CN 106857682A CN 201710061423 A CN201710061423 A CN 201710061423A CN 106857682 A CN106857682 A CN 106857682A
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water
weight portions
black spot
taking
acetum
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陈奇凌
郑强卿
王晶晶
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Xinjiang Academy of Agricultural and Reclamation Sciences
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Xinjiang Academy of Agricultural and Reclamation Sciences
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Priority to CN201710061423.8A priority Critical patent/CN106857682A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of biological control method of dense planting jujube garden black spot, biological agent is mainly configured to by shitosan, trichoderma viride, methyl jasmonate, acetic acid, organosilicone polyoxyethylene ether and ethanol jujube fruit is sprayed.Compared with prior art, the invention has the advantages that:1st, the present invention contains various bacteriostatic agents simultaneously, and the prevention effect to fungal diseases of plants is obvious;2nd, shitosan forms envelope on jujube fruit surface, and fruit surface area water is prevented while fungal infection is mitigated, and changes fungal infection condition, and effect is obvious;3rd, trichoderma viride can form the competition with black spot pathomycete, at the same wound with it, penetrated, it is parasitic, reach the effect for preventing pathogen from invading;4th, the resistance of plant itself can be improved using methyl jasmonate.

Description

A kind of biological control method of dense planting jujube garden black spot
Technical field
The present invention relates to a kind of biological control method of dense planting jujube garden black spot, belong to jujube tree disease control field.
Background technology
Jujube tree is the peculiar economic tree of China, and current area, up to 1,500,000 hectares, is the big dry fruit tree variety of China first.Closely Year Xinjiang turns into emerging jujube tree artificial growth area because of its natural environment regional superiority, produces that jujube quality is high, and red dates industry is As Xinjiang it is with fastest developing speed in recent years, covering is most wide, benefit is most prominent, the most significant forest and fruit of Huimin effect.Xinjiang Jujube garden is generally dense planting intensive culture, and fruit black spot occurs generally, to cause serious loss to peasants and workers, serious shadow in recent years Ring the sound development to red dates industry.The preventing and treating of jujube garden fruit black spot turned into produce in pressing issues the most.
, there is dark brown to black circle scab, more at fruit surface area water in early days in jujube fruit infection black spot.Disease Tissue is in dark brown hemispherical, and the strong junction of disease is easily peelable, above there is black mustiness thing.From in terms of Pathogenicity result, rod method Bacterium can also cause a disease mainly by wound infection without wound inoculation, illustrate also be infected by nature aperture, and high humility can cause disease Feelings are aggravated, therefore reduce jujube fruit superficial cut, and it is one of the key for preventing and treating the disease to reduce jujube fruit humidity environment.
In existing known technology, the preventing and treating to jujube black spot is mainly controlled using chemical method, chemical agriculture Because it has virulence strong, instant effect, the generation and the advantage for spreading that can rapidly suppress pest and disease damage turns into human social development to medicine Mark, be effectively protected and developed social production, Mancozeb, thiram, Propineb, Bravo etc. are commonly used in production and are entered Row preventing and treating.But asked because the use of chemical agent has residues of pesticides, environmental pollution, the agricultural chemicals resistance to the action of a drug and toxic hazard etc. Topic, and in recent years because climate change, jujube production later stage rainfall increases and humidity increase, and the state of an illness is developed rapidly, chemical prevention effect Fruit can not be satisfactory.
Therefore, a kind of biological control method of dense planting jujube garden black spot is suggested, and realizes that progressively substituted chemistry medicament makes With, and reach the purpose of sustainable development.
The content of the invention
The technical problems to be solved by the invention be jujube garden black spot preventing and treating during remain chemical agent to human body Harm and the pollution to environment, it is therefore an objective to a kind of biological control method of dense planting jujube garden black spot is provided.
To achieve these goals, the present invention uses following technical scheme:
A kind of biological control method of dense planting jujube garden black spot, at least comprises the following steps:
1) preparation of biological agent:
A, the acetic acid for taking 10~30 weight portions add water, and are configured to the acetum of concentration≤10%;
B, the shitosan for taking 30~50 weight portions add the acetum obtained by step a;
C, take 0.2~2 weight portion methyl jasmonate add 5~20 weight portions ethanol in, obtain the second of methyl jasmonate Alcoholic solution;
D, take 5~10 times of water dissolves of the trichoderma viride of 5~20 weight portions;
5~10 times of water described in step d are >=water that fully dissolves respective components;The water of 5~10 times of weight is advisable.
E, by obtained by step b, c, d solution mix, be subsequently adding the organosilicone polyoxyethylene ether of 0.5~2 weight portion;
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 3~5% be standard;
2) use of biological agent:
Spraying time be late August to early September, with high pressure atomizing utensil with jujube fruit be that object is uniformly slowly sprayed, make Liquid on jujube fruit is in the state to be dripped, and mu consumption is sprayed every time for 20-30Kg.
Spray and be preferably carried out continuously 2-3 times, every minor tick 7-10 days;
Spray selection in the morning or carried out the dusk time-division, be most with before at 9 points in the morning of local time and/or after at 8 points in afternoon It is good.
A kind of biological agent for preventing and treating dense planting jujube garden black spot, it is characterised in that prepared by procedure below:
A, the acetic acid for taking 10~30 weight portions add water, and are configured to the acetum of concentration≤10%;
B, the shitosan for taking 30~50 weight portions add the acetum obtained by step a;
C, take 0.2~2 weight portion methyl jasmonate add 5~20 parts by weight of ethanol in, obtain the ethanol of methyl jasmonate Solution;
D, the appropriate water dissolves of trichoderma viride for taking 5~20 weight portions;
Suitable quantity of water described in step d is >=water that fully dissolves respective components;The water of 5~10 times of weight is advisable.
E, by obtained by step b, c, d solution mix, be subsequently adding the organosilicone polyoxyethylene ether of 0.5~2 weight portion.
Shown by contrast test, test data is shown in table 1 in accompanying drawing, biological agent of the invention has very to jujube fruit black spot Obvious effect, diseased fruit rate is very low, and preventive effect is good, and biological agent is to human body and environmentally friendly, it is possible to use safely.Cause This, from the point of view of application effect, biological agent of the invention has a extensive future.
Compared with prior art, the invention has the advantages that:
1. the present invention contains various bacteriostatic agents simultaneously, and the prevention effect to fungal diseases of plants is obvious.
2. shitosan forms envelope on jujube fruit surface, and fruit surface area water is prevented while fungal infection is mitigated, and changes true Bacterium infection condition, effect is obvious.
3. trichoderma viride can form the competition with black spot pathomycete, at the same wound with it, penetrated, it is parasitic, reach The effect for preventing pathogen from invading.
4. the resistance of plant itself can be improved using methyl jasmonate.
Brief description of the drawings
Figure is the using effect contrast table of biological agent and chemical agent.
Specific embodiment
Embodiment 1:The preparation of biological agent:
A, the acetic acid for taking 10 weight portions add water, and are configured to the acetum that concentration is 10%;B, take 46 weight portions shell gather Sugar is added in the acetum obtained by step a;C, take 2 weight portions methyl jasmonate add 20 weight portions ethanol in, obtain jasmine The ethanol solution of jasmine acid methyl esters;D, take 5 times of water dissolves of weight of the trichoderma viride of 7 weight portions;E, by step b, c, d gained Solution mixing, be subsequently adding the organosilicone polyoxyethylene ether of 0.5 weight portion.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 5% be standard.
Embodiment 2:The preparation of biological agent:
A, the acetic acid for taking 12 weight portions add water, and are configured to the acetum that concentration is 3%;B, the shitosan for taking 32 weight portions In acetum obtained by addition step a;C, take 1.9 weight portions methyl jasmonate add 19 weight portions ethanol in, obtain jasmine The ethanol solution of jasmine acid methyl esters;D, take 7 times of water dissolves of weight of the trichoderma viride of 8 weight portions;E, by step b, c, d gained Solution mixing, be subsequently adding the organosilicone polyoxyethylene ether of 0.6 weight portion.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 3% be standard.
Embodiment 3:The preparation of biological agent:
A, the acetic acid for taking 14 weight portions add water, and are configured to the acetum that concentration is 4%;B, the shitosan for taking 36 weight portions In acetum obtained by addition step a;C, take 0.2 weight portion methyl jasmonate add 18 weight portions ethanol in, obtain jasmine The ethanol solution of jasmine acid methyl esters;D, take 8 times of water dissolves of weight of the trichoderma viride of 9 weight portions;E, by step b, c, d gained Solution mixing, be subsequently adding the organosilicone polyoxyethylene ether of 0.7 weight portion.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 5% be standard.
Embodiment 4:The preparation of biological agent:
A, the acetic acid for taking 16 weight portions add water, and are configured to the acetum that concentration is 9%;B, the shitosan for taking 40 weight portions In acetum obtained by addition step a;C, take 0.3 weight portion methyl jasmonate add 17 weight portions ethanol in, obtain jasmine The ethanol solution of jasmine acid methyl esters;D, take 9 times of water dissolves of weight of the trichoderma viride of 10 weight portions;E, by step b, c, d institutes The solution mixing for obtaining, is subsequently adding the organosilicone polyoxyethylene ether of 0.8 weight portion.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 4% be standard.
Embodiment 5:The preparation of biological agent:
A, the acetic acid for taking 18 weight portions add water, and are configured to the acetum that concentration is 8%;B, the shitosan for taking 50 weight portions In acetum obtained by addition step a;C, take 0.4 weight portion methyl jasmonate add 16 weight portions ethanol in, obtain jasmine The ethanol solution of jasmine acid methyl esters;D, take 10 times of water dissolves of weight of the trichoderma viride of 11 weight portions;E, by step b, c, d institutes The solution mixing for obtaining, is subsequently adding the organosilicone polyoxyethylene ether of 0.9 weight portion.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 3% be standard.
Embodiment 6:The preparation of biological agent:
A, the acetic acid for taking 20 weight portions add water, and are configured to the acetum that concentration is 7%;B, the shitosan for taking 48 weight portions In acetum obtained by addition step a;C, take 0.5 weight portion methyl jasmonate add 15 weight portions ethanol in, obtain jasmine The ethanol solution of jasmine acid methyl esters;D, take 6 times of water dissolves of weight of the trichoderma viride of 12 weight portions;E, by step b, c, d institutes The solution mixing for obtaining, is subsequently adding the organosilicone polyoxyethylene ether of 1.0 weight portions.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 5% be standard.
Embodiment 7:The preparation of biological agent:
A, the acetic acid for taking 22 weight portions add water, and are configured to the acetum that concentration is 6%;B, the shitosan for taking 46 weight portions In acetum obtained by addition step a;C, take 0.7 weight portion methyl jasmonate add 14 weight portions ethanol in, obtain jasmine The ethanol solution of jasmine acid methyl esters;D, take 5 times of water dissolves of weight of the trichoderma viride of 13 weight portions;E, by step b, c, d institutes The solution mixing for obtaining, is subsequently adding the organosilicone polyoxyethylene ether of 1.1 weight portions.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 4% be standard.
Embodiment 8:The preparation of biological agent:
A, the acetic acid for taking 24 weight portions add water, and are configured to the acetum that concentration is 5%;B, the shitosan for taking 44 weight portions In acetum obtained by addition step a;C, take 0.8 weight portion methyl jasmonate add 13 weight portions ethanol in, obtain jasmine The ethanol solution of jasmine acid methyl esters;D, take 10 times of water dissolves of weight of the trichoderma viride of 6 weight portions;E, by step b, c, d institutes The solution mixing for obtaining, is subsequently adding the organosilicone polyoxyethylene ether of 1.2 weight portions.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 3% be standard.
Embodiment 9:The preparation of biological agent:
A, the acetic acid for taking 26 weight portions add water, and are configured to the acetum that concentration is 10%;B, take 42 weight portions shell gather Sugar is added in the acetum obtained by step a;C, take 1.0 weight portions methyl jasmonate add 12 weight portions ethanol in, obtain The ethanol solution of methyl jasmonate;D, take 9 times of water dissolves of weight of the trichoderma viride of 5 weight portions;E, by step b, c, d institutes The solution mixing for obtaining, is subsequently adding the organosilicone polyoxyethylene ether of 1.3 weight portions.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 5% be standard.
Embodiment 10:The preparation of biological agent:
A, the acetic acid for taking 28 weight portions add water, and are configured to the acetum that concentration is 9%;B, the shitosan for taking 40 weight portions In acetum obtained by addition step a;C, take 1.2 weight portions methyl jasmonate add 11 weight portions ethanol in, obtain jasmine The ethanol solution of jasmine acid methyl esters;D, take 8 times of water dissolves of weight of the trichoderma viride of 14 weight portions;E, by step b, c, d institutes The solution mixing for obtaining, is subsequently adding the organosilicone polyoxyethylene ether of 1.4 weight portions.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 4% be standard.
Embodiment 11:The preparation of biological agent:
A, the acetic acid for taking 30 weight portions add water, and are configured to the acetum that concentration is 8%;B, the shitosan for taking 38 weight portions In acetum obtained by addition step a;C, take 0.3 weight portion methyl jasmonate add 10 weight portions ethanol in, obtain jasmine The ethanol solution of jasmine acid methyl esters;D, take 7 times of water dissolves of weight of the trichoderma viride of 15 weight portions;E, by step b, c, d institutes The solution mixing for obtaining, is subsequently adding the organosilicone polyoxyethylene ether of 1.5 weight portions.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 3% be standard.
Embodiment 12:The preparation of biological agent:
A, the acetic acid for taking 26 weight portions add water, and are configured to the acetum that concentration is 7%;B, the shitosan for taking 36 weight portions In acetum obtained by addition step a;C, take 1.4 weight portions methyl jasmonate add 9 weight portions ethanol in, obtain jasmine The ethanol solution of sour methyl esters;D, take the 5-10 times of water dissolves of weight of the trichoderma viride of 16 weight portions;E, by step b, c, d institutes The solution mixing for obtaining, is subsequently adding the organosilicone polyoxyethylene ether of 1.6 weight portions.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 5% be standard.
Embodiment 13:The preparation of biological agent:
A, the acetic acid for taking 22 weight portions add water, and are configured to the acetum that concentration is 6%;B, the shitosan for taking 34 weight portions In acetum obtained by addition step a;C, take 1.5 weight portions methyl jasmonate add 8 weight portions ethanol in, obtain jasmine The ethanol solution of sour methyl esters;D, take 6 times of water dissolves of weight of the trichoderma viride of 20 weight portions;E, by step b, c, d gained Solution mixing, be subsequently adding the organosilicone polyoxyethylene ether of 1.7 weight portions.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 4% be standard.
Embodiment 14:The preparation of biological agent:
A, the acetic acid for taking 18 weight portions add water, and are configured to the acetum that concentration is 5%;B, the shitosan for taking 32 weight portions In acetum obtained by addition step a;C, take 1.6 weight portions methyl jasmonate add 6 weight portions ethanol in, obtain jasmine The ethanol solution of sour methyl esters;D, take 7 times of water dissolves of weight of the trichoderma viride of 19 weight portions;E, by step b, c, d gained Solution mixing, be subsequently adding the organosilicone polyoxyethylene ether of 1.8 weight portions.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 5% be standard.
Embodiment 15:The preparation of biological agent:
A, the acetic acid for taking 14 weight portions add water, and are configured to the acetum that concentration is 4%;B, the shitosan for taking 30 weight portions In acetum obtained by addition step a;C, take 1.7 weight portions methyl jasmonate add 5 weight portions ethanol in, obtain jasmine The ethanol solution of sour methyl esters;D, take 8 times of water dissolves of weight of the trichoderma viride of 17 weight portions;E, by step b, c, d gained Solution mixing, be subsequently adding the organosilicone polyoxyethylene ether of 2 weight portions.
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water is with chitosan concentration For 3% be standard.
Embodiment 16:It is preventing and treating dense planting jujube garden black spot, jujube tree is sprayed with the biological agent described in embodiment 1~15 Apply, Southern Xinjiang late August, to early September, sprays and is carried out continuously 3 times, every minor tick 10 days, a mu consumption is sprayed every time is 20Kg, spray is carried out before at 9 points in the morning of local time;High pressure atomizing utensil is that object uniformly slowly enters with jujube fruit when spraying OK, the spray liquid on jujube fruit is made to occur being intended to drop-wise state.
Embodiment 17:It is preventing and treating dense planting jujube garden black spot, jujube tree is sprayed with the biological agent described in embodiment 1~15 Apply, Southern Xinjiang late August, to early September, sprays and is carried out continuously 2 times, every minor tick 9 days sprays mu consumption for 23Kg every time, Spray is carried out after at 8 points in the afternoon of local time;When spraying with high pressure atomizing utensil with jujube fruit be that object is uniformly slowly carried out, make Spray liquid on jujube fruit occurs being intended to drop-wise state.
Embodiment 18:It is preventing and treating dense planting jujube garden black spot, jujube tree is sprayed with the biological agent described in embodiment 1~15 Apply, Southern Xinjiang late August, to early September, sprays and is carried out continuously 3 times, every minor tick 8 days sprays mu consumption for 25Kg every time, Spray before at 9 points in the morning of local time and carried out after at 8 points in afternoon;High pressure atomizing utensil is uniform object with jujube fruit when spraying Slowly carry out, make the spray liquid on jujube fruit occur being intended to drop-wise state.
Embodiment 19:It is preventing and treating dense planting jujube garden black spot, jujube tree is sprayed with the biological agent described in embodiment 1~15 Apply, Southern Xinjiang late August, to early September, sprays and is carried out continuously 2 times, every minor tick 8 days sprays mu consumption for 27Kg every time, Spray is carried out before at 9 points in the morning of local time;When spraying with high pressure atomizing utensil with jujube fruit be that object is uniformly slowly carried out, make Spray liquid on jujube fruit occurs being intended to drop-wise state.
Embodiment 20:It is preventing and treating dense planting jujube garden black spot, jujube tree is sprayed with the biological agent described in embodiment 1~15 Apply, Southern Xinjiang late August, to early September, sprays and is carried out continuously 3 times, every minor tick 7 days sprays mu consumption for 30Kg every time, Spray is carried out after at 8 points in the afternoon of local time;When spraying with high pressure atomizing utensil with jujube fruit be that object is uniformly slowly carried out, make Spray liquid on jujube fruit occurs being intended to drop-wise state.
Above-described is only the preferred embodiment of the present invention, it is noted that for one of ordinary skill in the art For, without departing from the concept of the premise of the invention, some changes and improvements can also be made, these belong to the present invention Protection domain.

Claims (5)

1. a kind of biological control method of dense planting jujube garden black spot, it is characterised in that at least comprise the following steps:
1) preparation of biological agent:
A, the acetic acid for taking 10~30 weight portions add water, and are configured to the acetum of concentration≤10%;
B, the shitosan for taking 30~50 weight portions add the acetum obtained by step a;
C, take 0.2~2 weight portion methyl jasmonate add 5~20 weight portions ethanol in, the ethanol for obtaining methyl jasmonate is molten Liquid;
D, take 5~10 times of water dissolves of the trichoderma viride of 5~20 weight portions;
5~10 times of water described in step d are >=water that fully dissolves respective components;
E, by obtained by step b, c, d solution mix, be subsequently adding the organosilicone polyoxyethylene ether of 0.5~2 weight portion;
When using, the solution obtained by step e is diluted with water to biological agent and is sprayed, amount of water with chitosan concentration be 3 ~5% is standard;
2) use of biological agent:
Spraying time is late August to early September, with jujube fruit for object is uniformly slowly sprayed, makes the liquid on jujube fruit be in be intended to drip State, mu consumption is sprayed every time for 20~30Kg.
2. the biological control method of dense planting jujube garden black spot as claimed in claim 1, it is characterised in that described spraying is continuous Carry out 2~3 times, every minor tick 7~10 days.
3. the biological control method of dense planting jujube garden black spot as claimed in claim 1 or 2, it is characterised in that described sprays Time is early morning or the dusk time-division.
4. the biological control method of dense planting jujube garden black spot as claimed in claim 1 or 2, it is characterised in that described sprays Time was before at 9 points in morning local time and/or after at 8 points in afternoon.
5. it is a kind of prevent and treat dense planting jujube garden black spot biological agent, it is characterised in that by procedure below prepare:
A, the acetic acid for taking 10~30 weight portions add water, and are configured to the acetum of concentration≤10%;
B, the shitosan for taking 30~50 weight portions add the acetum obtained by step a;
C, take 0.2~2 weight portion methyl jasmonate add 5~20 weight portions ethanol in, the ethanol for obtaining methyl jasmonate is molten Liquid;
D, the appropriate water dissolves of trichoderma viride for taking 5~20 weight portions;
Suitable quantity of water described in step d is >=water that fully dissolves respective components;The water of 5~10 times of weight is advisable.
E, by obtained by step b, c, d solution mix, be subsequently adding the organosilicone polyoxyethylene ether of 0.5~2 weight portion.
CN201710061423.8A 2017-01-26 2017-01-26 A kind of biological control method of dense planting jujube garden black spot Pending CN106857682A (en)

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CN113475531A (en) * 2021-06-30 2021-10-08 海南金雨丰生物工程有限公司 Actinomycete complex microbial inoculum and preparation method and application thereof

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CN102925367A (en) * 2012-10-24 2013-02-13 山东省林业科学研究院 Trichoderma viride strain and application thereof
CN105594751A (en) * 2016-01-28 2016-05-25 黄河三角洲京博化工研究院有限公司 Trichoderma spore preparation and preparation method thereof

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