CN106856973A - A kind of breeding method of efficient enhancing alfalfa-leaf function - Google Patents
A kind of breeding method of efficient enhancing alfalfa-leaf function Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses a kind of breeding method of efficient enhancing alfalfa-leaf function, including step one：Soil treatment and environmental selection；Step 2：Seeds preprocess；Step 3：Seeding and seedling raising；Step 4：Fertilising growth promoting；It is pre-buried by soil carrying out base fertilizer, improve the fertility of soil and be accurate to the nutrient soil depth of planting depth, control the intensity of illumination of planting environment, temperature, humidity, cut nutrition immersion treatment is carried out to seed, strengthen the developmental potency of seed, leaf growth to clover provides good nutrient environment, interval division is carried out during sowing, leaf portion growth to alfalfa retains sufficient space, it is regular in alfalfa nursery growth course to apply fertilizer and hormone, it is effectively promoted the leaf function of clover, improve the increment of cloverleaf, the effective biomass using cloverleaf, with good market value.
Description
Technical field
The invention belongs to clover Cultivating techniques field, specifically it is related to a kind of efficient enhancing alfalfa-leaf function
Breeding method.
Background technology
Alfalfa, original name：Alfalfa, also known as clover, Rosales, pulse family, clover belong to perennial herb, and root is sturdy, deep
Underground layer, root neck is flourishing.Stem is upright, grow thickly so that prostration, four prismatics, without hair or micro- by pubescence, branches and leaves are luxuriant.Seed is avette,
It is long 1-2.5 millimeters, smooth, yellow or brown.The month at florescence 5-7, the fruiting period 6-8 months.Originate in Asia Minor, Iran, Transcaucasia
One band.There is cultivation all over the world or in semi-wild state.It is born in the ground such as field side, roadside, wilderness, grassland, riverbank and cheuch.It is purple
Russian fenugreek herb is rich in high-quality dietary fiber, edible protein, multivitamin (including vitamin B, vitamin C, vitamin E etc.), many
Plant the bioactive ingredients such as beneficial mineral matter and saponin(e, flavonoids, carotenoid, phenolic acid.Eurasia and the world are each
Plantation is feed and herbage extensively for state.
Clover functional trait is by observation or measures the biological characteristics for obtaining, and clover adapts to specific environment and table
Now certain functional trait is that clover continues coefficient result in particular growth stage various processes and environmental condition, because
This, clover also has time frame coefficient to the adaptability of environment.At present, scholars from time-variance angle to the photosynthetic spy of clover
Property, the physiological and biochemical procedure such as element circular, material accumulation studied, it is found that clover Net Photosynthetic Rate Daily variation is in " bimodal "
Curve, the reabsorption efficiency of alfalfa-leaves nitrogen is first raised with the increase at clover age and reduced afterwards, and the reabsorption of phosphorus and potassium element
Efficiency is in rising trend.And studying cloverleaf function helps to understand the herbaceous plant adaptive strategy taken to environment change.
Alfalfa is a kind of excellent Perennial legume forages, studies clover Bomass allocation, morphological feature under different cultivating processes
And growth rhythm, inquire into the functional trait change that clover environment is adapted to, Longdong alfalfa biomass point from the angle of time frame coefficient
With with the change of leaf functional trait, not only inquired into the Adaptive mechanism of Longdong alfalfa from time dynamic angle, and with plant by
Gradually reach maturity, Longdong alfalfa root weight, stem weight, Ye Chong, hat weight, gross weight, root biomass ratio, root/shoot ratio, leaf area, leaf area refer to
The data such as number have critically important Research Significance for its trophism and yield.
The content of the invention
Present invention solves the technical problem that there is provided a kind of comprehensive by soil regulation, Seed Treatment, plantation and fertilising
Effect, efficiently to strengthen the breeding method of alfalfa-leaf function.
The technical scheme is that：A kind of breeding method of efficient enhancing alfalfa-leaf function, comprises the following steps：
Step one：Soil treatment and environmental selection：The impurity such as the weeds in soil, stone are removed totally, in soil table
Face carries out basal dressing, and described base manure is Diammonium phosphate (DAP), and dose is 100-120kg/hm2, is then ploughed, and depth of ploughing is 5-
8cm, thin rake makes soil surfacing, and soil is soft, adjusts P in soil H values, then sprays so that the water content of soil is 20-
30%；Planted in the greenhouse of temperature-controllable, it is 20-30 DEG C to control environment temperature, and daylight degree is 7000-11000lx, light
It is 12-16h according to the time；
Step 2：Seeds preprocess：The alfalfa seed of full grains N/D is selected, is cleaned with clear water, remove table
Face impurity, is soaked seed 3-5 hours in 20-25 DEG C of water temperature, and the seed of alfalfa then is carried out into broken skin treatment, and cut length is
0.3-0.5 times of seed sizes, is put into nutrient solution, and 8-12h is soaked under the conditions of 23-25 DEG C, standby；
Step 3：Seeding and seedling raising：Described soil is carried out into interval division, described interval is square, and each is interval
Size be 20x20cm, retain sufficient space to the leaf portion growth of alfalfa, effectively control weeds produce, and make herbicide
Can sufficiently be contacted with weeds, facilitate field management and irrigation, can in good time carried out draining, return the work such as soil, supplement base manure,
Each interval topsoil is loose, then sowed, application rate 15-25g/m2, earthing is after planting answered, make alfalfa
Seed contacted with soil, thickness of earth covering is 2-3mm, be keep ground moistening, at the nursery initial stage, carry out sprinkling irrigation and water, every mu
Irrigation amount is 1200-1500L, keeps upper soll layer ground moistening, to ensure to emerge, unglazed cultivation is carried out in 5 days after planting,
Dim light cultivation is carried out within 5-10 days, normal illumination cultivation is carried out after 10 days, at 20-25 DEG C, humid control is in 60- for temperature control
80%.
Step 4：Fertilising growth promoting：When alfalfa nursery grows into 10-15cm, applied fertilizer, dose is every mu
10-12 kilograms of two ammoniums and 30-40 kilograms of calcium superphosphate, after the completion of fertilising, the sprinkling growth to nursery promotes fertilizer, and usage amount is every
6-8 kilograms of mu, after alfalfa is cradled and when growing into 15-20 centimeters, is applied fertilizer again, uses bacterium solution organic fertilizer
Often wheel applies 5-20kg/ mus.
Further, the preparation method of described nutrient solution is：30-50 parts of fern root is taken, the clear of 8-12 times of quality is soaked in
3-5 days in water, then water is outwelled, fern root is put into juice extractor, the water for rejoining 3-5 times of quality is squeezed the juice, heated
Mixture after squeezing the juice extracts 1-2h to seething with excitement, and then filters, and filtrate is collected, when liquid temperature degree to be filtered is down to room temperature, toward filtrate
It is separately added into 25mg/L NH4NO3、28mg/L KNO3、6.5mg/L CaCl2.2H2O、3.6mg/L MgSO4.7H2O、2.5mg/L
KH2PO4With 6-12g/L NaCl, after being well mixed, that is, described nutrient solution is obtained.
Further, the light index in described dim light area is 50-60 luxs, and the light index of highlights is 80-100
Lux, the intensity of illumination of effective regulation different time sections has good facilitation for the nursery growth of clover.
Further, it is concentrated liquid fertilizer that described growth promotes fertilizer, and component content is：6- benzylaminopurines 45g/L,
Nitrogen 35g/L, phosphoric anhydride 18g/L, potassium oxide 15.6g/L, magnesia 1.0g/L, sulphur 1.5g/L.
Further, described more than bacterium solution organic fertilizer N P and K total content 15wt%, more than content of organic matter 70wt%,
Effective total viable count 0.2 hundred million/gram more than.
Further, described P in soil H values are 6.5-6.7, make soil conservation faintly acid.
Further, described alfalfa is Longdong alfalfa.
Further, described greenhouse is light, temperature, the planting greenhouse of the closed Automated condtrol of wet intelligentized control method,
Described light intelligentized control method is realized by the Solar lamp being arranged at the top of greenhouse and intelligent diaphragm system, sensed by illumination
Device detects intensity of illumination, and the data transfer that will be detected to control of intelligent terminal is analyzed treatment, intelligent screening is then controlled again
Photosystem carries out intensity of illumination regulation；Described temperature and humidity intelligentized control method is to obtain data by sensor respectively to believe
Breath, is then communicated to control of intelligent terminal, and temperature adjustment realizes that humidity regulation is by spray by air-conditioning system and electric heating system
Mist humidification device realizes that described control of intelligent terminal can carry out remote control by wireless network.
Compared with prior art, beneficial effects of the present invention are embodied in：It is pre-buried by carrying out to soil base fertilizer, improve soil
Fertility and be accurate to the nutrient soil depth of planting depth, intensity of illumination, temperature, the humidity of planting environment are controlled, to seed
Cut nutrition immersion treatment is carried out, strengthens the developmental potency of seed, the leaf growth to clover provides good nutrient environment, sowing
Shi Jinhang interval divisions, sufficient space is retained to the leaf portion growth of alfalfa, and effectively control weeds produce, make herbicide and
Weeds can sufficiently contact, and facilitate field management and irrigation, regular in alfalfa nursery growth course to apply fertilizer
And hormone, the leaf function of clover is effectively promoted, improve the increment of cloverleaf, the effective biomass using cloverleaf, tool
There is good market value.
Specific embodiment
Embodiment 1：A kind of breeding method of efficient enhancing alfalfa-leaf function, comprises the following steps：
Step one：Soil treatment and environmental selection：The impurity such as the weeds in soil, stone are removed totally, in soil table
Face carries out basal dressing, and base manure is Diammonium phosphate (DAP), and dose is 100kg/hm2, then to plough, depth of ploughing is 5cm, and thin rake makes soil
Ground surface is smooth, and soil is soft, regulation P in soil H values are 6.5, make soil conservation faintly acid, are then sprayed so that soil contains
Water is 20%；Planted in the greenhouse of temperature-controllable, it is 20 DEG C to control environment temperature, and daylight degree is 7000lx, illumination
Time is 12h；
Step 2：Seeds preprocess：The alfalfa seed of full grains N/D is selected, alfalfa is Gansu Province east lucerne
Mu, is cleaned with clear water, removes surface impurity, is soaked seed 3 hours in 20 DEG C of water temperatures, and the seed of alfalfa then is carried out into broken skin
Treatment, cut length is 0.3 times of seed sizes, is put into nutrient solution, and 8h is soaked under the conditions of 23 DEG C, standby；
Step 3：Seeding and seedling raising：Soil is carried out into interval division, interval is square, and each interval size is
20x20cm, sufficient space is retained to the leaf portion growth of alfalfa, and effectively control weeds produce, and makes herbicide and the weeds can
Sufficiently to contact, facilitate field management and irrigation, can in good time carry out draining, the work such as soil, supplement base manure be returned, by each area
Between topsoil it is loose, then sowed, application rate 15g/m2, earthing is after planting answered, make the seed of alfalfa and soil
Earth is contacted, and thickness of earth covering is 2mm, to keep ground moistening, at the nursery initial stage, is carried out sprinkling irrigation and is watered, and every mu of irrigation amount is
1200L, keeps upper soll layer ground moistening, to ensure to emerge, unglazed cultivation is carried out in 5 days after planting, carries out dim light training within 5 days
Educate, normal illumination cultivation is carried out after 10 days, the light index in dim light area is 50 luxs, and the light index of highlights is 80 Le
Ke Si, the intensity of illumination of effective regulation different time sections has good facilitation, temperature control for the nursery growth of clover
, at 20 DEG C, humid control is 60% for system.
Step 4：Fertilising growth promoting：When alfalfa nursery grows into 10cm, applied fertilizer, dose is every mu 10 public
Two ammoniums of jin and 30 kilograms of calcium superphosphate, after the completion of fertilising, the sprinkling growth to nursery promotes fertilizer, and usage amount is 6 kilograms every mu, raw
Long to promote fertilizer fertile for concentrated liquid, component content is：6- benzylaminopurines 45g/L, nitrogen 35g/L, phosphoric anhydride 18g/L, potassium oxide
15.6g/L, magnesia 1.0g/L, sulphur 1.5g/L, after alfalfa is cradled and when growing into 15 centimeters, are applied again
Fertilizer, is often taken turns using 5-20kg/ mus, more than bacterium solution organic fertilizer N P and K total content 15wt% using bacterium solution organic fertilizer, and organic matter contains
Amount more than 70wt%, effective total viable count 0.2 hundred million/gram more than.
Wherein, the preparation method of nutrient solution is：30 parts of fern root is taken, is soaked in 3 days in 8 times of clear water of quality, then by water
Outwell, fern root be put into juice extractor, the water for rejoining 3 times of quality is squeezed the juice, the mixture after heating is squeezed the juice extremely seethes with excitement,
1h is extracted, is then filtered, collect filtrate, when liquid temperature degree to be filtered is down to room temperature, 25mg/L NH are separately added into toward filtrate4NO3、
28mg/L KNO3、6.5mg/L CaCl2.2H2O、3.6mg/L MgSO4.7H2O、2.5mg/L KH2PO4With 6g/L NaCl, mix
After closing uniformly, that is, obtain nutrient solution.
Greenhouse is light, temperature, the planting greenhouse of the closed Automated condtrol of wet intelligentized control method, and light intelligentized control method passes through
The Solar lamp and intelligent diaphragm system being arranged at the top of greenhouse are realized, by optical sensor detection intensity of illumination, will detected
To data transfer be analyzed treatment to control of intelligent terminal, then control intelligent diaphragm system to carry out intensity of illumination tune again
Section；Temperature and humidity intelligentized control method is to obtain data message by sensor respectively, is then communicated to control of intelligent terminal, temperature
Degree regulation realizes that humidity regulation is realized by spraying humidifier, control of intelligent terminal by air-conditioning system and electric heating system
Remote control can be carried out by wireless network.
Embodiment 2：A kind of breeding method of efficient enhancing alfalfa-leaf function, comprises the following steps：
Step one：Soil treatment and environmental selection：The impurity such as the weeds in soil, stone are removed totally, in soil table
Face carries out basal dressing, and base manure is Diammonium phosphate (DAP), and dose is 110kg/hm2, then to plough, depth of ploughing is 6.5cm, and thin rake makes
Soil surfacing, soil is soft, and regulation P in soil H values are 6.6, make soil conservation faintly acid, are then sprayed so that soil
Water content is 25%；Planted in the greenhouse of temperature-controllable, it is 25 DEG C to control environment temperature, and daylight degree is 9000lx, light
It is 14h according to the time；
Step 2：Seeds preprocess：The alfalfa seed of full grains N/D is selected, alfalfa is Gansu Province east lucerne
Mu, is cleaned with clear water, removes surface impurity, is soaked seed 4 hours in 22.5 DEG C of water temperatures, is then broken the seed of alfalfa
Skin treatment, cut length is 0.4 times of seed sizes, is put into nutrient solution, and 10h is soaked under the conditions of 24 DEG C, standby；
Step 3：Seeding and seedling raising：Soil is carried out into interval division, interval is square, and each interval size is
20x20cm, sufficient space is retained to the leaf portion growth of alfalfa, and effectively control weeds produce, and makes herbicide and the weeds can
Sufficiently to contact, facilitate field management and irrigation, can in good time carry out draining, the work such as soil, supplement base manure be returned, by each area
Between topsoil it is loose, then sowed, application rate 20g/m2 after planting answers earthing, makes the seed of alfalfa and soil
Earth is contacted, and thickness of earth covering is 2.5mm, to keep ground moistening, at the nursery initial stage, is carried out sprinkling irrigation and is watered, and every mu of irrigation amount is
1350L, keeps upper soll layer ground moistening, to ensure to emerge, unglazed cultivation is carried out in 5 days after planting, carries out dim light training within 7 days
Educate, normal illumination cultivation is carried out after 10 days, the light index in dim light area is 55 luxs, and the light index of highlights is 90 Le
Ke Si, the intensity of illumination of effective regulation different time sections has good facilitation, temperature control for the nursery growth of clover
, at 22.5 DEG C, humid control is 70% for system.
Step 4：Fertilising growth promoting：When alfalfa nursery grows into 12.5cm, applied fertilizer, dose is every mu 11
Kilogram two ammoniums and 35 kilograms of calcium superphosphate, after the completion of fertilising, the sprinkling growth to nursery promotes fertilizer, and usage amount is 7 kilograms every mu,
It is concentrated liquid fertilizer that growth promotes fertilizer, and component content is：6- benzylaminopurines 45g/L, nitrogen 35g/L, phosphoric anhydride 18g/L, oxidation
Potassium 15.6g/L, magnesia 1.0g/L, sulphur 1.5g/L, after alfalfa is cradled and when growing into 18 centimeters, are carried out again
Fertilising, is often taken turns using 10kg/ mus, more than bacterium solution organic fertilizer N P and K total content 15wt% using bacterium solution organic fertilizer, and organic matter contains
Amount more than 70wt%, effective total viable count 0.2 hundred million/gram more than.
Wherein, the preparation method of nutrient solution is：40 parts of fern root is taken, is soaked in 4 days in 10 times of clear water of quality, then by water
Outwell, fern root be put into juice extractor, the water for rejoining 4 times of quality is squeezed the juice, the mixture after heating is squeezed the juice extremely seethes with excitement,
1.5h is extracted, is then filtered, collect filtrate, when liquid temperature degree to be filtered is down to room temperature, 25mg/L NH are separately added into toward filtrate4NO3、
28mg/L KNO3、6.5mg/L CaCl2.2H2O、3.6mg/L MgSO4.7H2O、2.5mg/L KH2PO4With 9g/L NaCl, mix
After closing uniformly, that is, obtain nutrient solution.
Greenhouse is light, temperature, the planting greenhouse of the closed Automated condtrol of wet intelligentized control method, and light intelligentized control method passes through
The Solar lamp and intelligent diaphragm system being arranged at the top of greenhouse are realized, by optical sensor detection intensity of illumination, will detected
To data transfer be analyzed treatment to control of intelligent terminal, then control intelligent diaphragm system to carry out intensity of illumination tune again
Section；Temperature and humidity intelligentized control method is to obtain data message by sensor respectively, is then communicated to control of intelligent terminal, temperature
Degree regulation realizes that humidity regulation is realized by spraying humidifier, control of intelligent terminal by air-conditioning system and electric heating system
Remote control can be carried out by wireless network.
Embodiment 3：A kind of breeding method of efficient enhancing alfalfa-leaf function, comprises the following steps：
Step one：Soil treatment and environmental selection：The impurity such as the weeds in soil, stone are removed totally, in soil table
Face carries out basal dressing, and base manure is Diammonium phosphate (DAP), and dose is 120kg/hm2, then to plough, depth of ploughing is 8cm, and thin rake makes soil
Ground surface is smooth, and soil is soft, regulation P in soil H values are 6.7, make soil conservation faintly acid, are then sprayed so that soil contains
Water is 30%；Planted in the greenhouse of temperature-controllable, it is 30 DEG C to control environment temperature, and daylight degree is 11000lx, illumination
Time is 16h；
Step 2：Seeds preprocess：The alfalfa seed of full grains N/D is selected, alfalfa is Gansu Province east lucerne
Mu, is cleaned with clear water, removes surface impurity, is soaked seed 5 hours in 25 DEG C of water temperatures, and the seed of alfalfa then is carried out into broken skin
Treatment, cut length is 0.5 times of seed sizes, is put into nutrient solution, and 12h is soaked under the conditions of 25 DEG C, standby；
Step 3：Seeding and seedling raising：Soil is carried out into interval division, interval is square, and each interval size is
20x20cm, sufficient space is retained to the leaf portion growth of alfalfa, and effectively control weeds produce, and makes herbicide and the weeds can
Sufficiently to contact, facilitate field management and irrigation, can in good time carry out draining, the work such as soil, supplement base manure be returned, by each area
Between topsoil it is loose, then sowed, application rate 25g/m2, earthing is after planting answered, make the seed of alfalfa and soil
Earth is contacted, and thickness of earth covering is 3mm, to keep ground moistening, at the nursery initial stage, is carried out sprinkling irrigation and is watered, and every mu of irrigation amount is
1500L, keeps upper soll layer ground moistening, to ensure to emerge, unglazed cultivation is carried out in 5 days after planting, carries out dim light within 10 days
Cultivate, normal illumination cultivation is carried out after 10 days, the light index in dim light area is 60 luxs, and the light index of highlights is
100 luxs, the intensity of illumination of effective regulation different time sections has good facilitation for the nursery growth of clover, temperature
At 25 DEG C, humid control is 80% for degree control.
Step 4：Fertilising growth promoting：When alfalfa nursery grows into 15cm, applied fertilizer, dose is every mu 12 public
Two ammoniums of jin and 40 kilograms of calcium superphosphate, after the completion of fertilising, the sprinkling growth to nursery promotes fertilizer, and usage amount is 8 kilograms every mu, raw
Long to promote fertilizer fertile for concentrated liquid, component content is：6- benzylaminopurines 45g/L, nitrogen 35g/L, phosphoric anhydride 18g/L, potassium oxide
15.6g/L, magnesia 1.0g/L, sulphur 1.5g/L, after alfalfa is cradled and when growing into 20 centimeters, are applied again
Fertilizer, is often taken turns using 20kg/ mus, more than bacterium solution organic fertilizer N P and K total content 15wt%, the content of organic matter using bacterium solution organic fertilizer
More than 70wt%, effective total viable count 0.2 hundred million/gram more than.
Wherein, the preparation method of nutrient solution is：50 parts of fern root is taken, is soaked in 5 days in 12 times of clear water of quality, then by water
Outwell, fern root be put into juice extractor, the water for rejoining 5 times of quality is squeezed the juice, the mixture after heating is squeezed the juice extremely seethes with excitement,
2h is extracted, is then filtered, collect filtrate, when liquid temperature degree to be filtered is down to room temperature, 25mg/L NH are separately added into toward filtrate4NO3、
28mg/L KNO3、6.5mg/L CaCl2.2H2O、3.6mg/L MgSO4.7H2O、2.5mg/L KH2PO4With 12g/L NaCl, mix
After closing uniformly, that is, obtain nutrient solution.
Greenhouse is light, temperature, the planting greenhouse of the closed Automated condtrol of wet intelligentized control method, and light intelligentized control method passes through
The Solar lamp and intelligent diaphragm system being arranged at the top of greenhouse are realized, by optical sensor detection intensity of illumination, will detected
To data transfer be analyzed treatment to control of intelligent terminal, then control intelligent diaphragm system to carry out intensity of illumination tune again
Section；Temperature and humidity intelligentized control method is to obtain data message by sensor respectively, is then communicated to control of intelligent terminal, temperature
Degree regulation realizes that humidity regulation is realized by spraying humidifier, control of intelligent terminal by air-conditioning system and electric heating system
Remote control can be carried out by wireless network.
Longdong alfalfa leaf functional trait and biomass test experience：Trained with the embodiment of the present invention 1, embodiment 2, embodiment 3
The alfalfa that the alfalfa educated is planted with common implantation methods is contrasted, and analyzes its leaf function and corresponding biomass.
1. materials and methods
1.1 experimental designs：Three kinds of embodiments of the invention and common cultivating process are observed, selection health is planted
Strain, the 30th day (branching stage) first sub-sampling after emerging took a sample every 6 days later, entered pregnant flower bud phase within the 84th day after emerging,
96th day initial bloom stage after emerging, the 114th day full-bloom stage takes last time sample after emerging, and four groups are divided into after sampling.
1.2 index determinings
Biomass：Leaf area (LA)：Leaf area per plant is determined with Win-FOLIA Leaf area determinations instrument.
1.3 data statistic analysis
Leaf area (LA) average data of table 1 (mm2)
| Classification | Branching stage | Pregnant flower bud phase | Initial bloom stage |
| Embodiment 1 | 23.56 | 69.14 | 108.36 |
| Embodiment 2 | 28.94 | 70.80 | 115.42 |
| Embodiment 3 | 25.32 | 63.44 | 99.86 |
| Common breeding method | 22.58 | 43.22 | 65.28 |
2. result and analysis：By data above as can be seen that the leaf of the alfalfa that three kinds of embodiments of the invention are cultivated
Area is not obvious with the alfalfa difference that the common method cultivated is cultivated in branching stage, but increased in pregnant flower bud phase difference
Greatly, in the leaf area significant difference of initial bloom stage, therefore, it can show that breeding method of the invention can show compared with commonsense method
The leaf area for improving alfalfa is write, while strengthening leaf function and its biomass.
Finally it should be noted that：The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
The present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those within the art that：It still may be used
Modified with to the technical scheme described in previous embodiment, or equivalent is carried out to which part technical characteristic；And
These modifications are replaced, and do not make the spirit and model of the essence disengaging embodiment of the present invention technical scheme of appropriate technical solution
Enclose.
Claims (7)
1. it is a kind of it is efficient enhancing alfalfa-leaf function breeding method, it is characterised in that described method is comprised the following steps：
Step one：Soil treatment and environmental selection：The impurity such as the weeds in soil, stone are removed totally, is entered in soil surface
Row basal dressing, described base manure is Diammonium phosphate (DAP), and dose is 100-120kg/hm2, then to plough, depth of ploughing is 5-8cm,
Thin rake makes soil surfacing, and soil is soft, adjusts P in soil H values, then sprays so that the water content of soil is 20-30%；
Planted in the greenhouse of temperature-controllable, it is 20-30 DEG C to control environment temperature, and daylight degree is 7000-11000lx, during illumination
Between be 12-16h；
Step 2：Seeds preprocess：The alfalfa seed of full grains N/D is selected, is cleaned with clear water, removal surface is miscellaneous
Matter, is soaked seed 3-5 hours in 20-25 DEG C of water temperature, and the seed of alfalfa then is carried out into broken skin treatment, and cut length is seed
0.3-0.5 times of size, is put into nutrient solution, and 8-12h is soaked under the conditions of 23-25 DEG C, standby；
Step 3：Seeding and seedling raising：Described soil is carried out into interval division, described interval is square, each interval chi
Very little is 20x20cm, and each interval topsoil is loose, is then sowed, application rate 15-25g/m2, should after planting cover
Soil, makes the seed of alfalfa be contacted with soil, and thickness of earth covering is 2-3mm, to keep ground moistening, at the nursery initial stage, carries out
Sprinkling irrigation is watered, and every mu of irrigation amount is 1200-1500L, keeps upper soll layer ground moistening, to ensure to emerge, in 5 days after planting
Unglazed cultivation is carried out, dim light cultivation is carried out within 5-10 days, normal illumination cultivation is carried out after 10 days, temperature control is wet at 20-25 DEG C
Degree control is in 60-80%；
Step 4：Fertilising growth promoting：When alfalfa nursery grows into 10-15cm, applied fertilizer, dose is every mu of 10-12
Kilogram two ammoniums and 30-40 kilograms of calcium superphosphate, after the completion of fertilising, fertilizer are promoted to nursery sprinkling growth, and usage amount is every mu of 6-8 public affairs
Jin, after alfalfa is cradled and when growing into 15-20 centimeters, is applied fertilizer again, is often taken turns using bacterium solution organic fertilizer and applied
With 5-20kg/ mus.
2. the breeding method of a kind of efficient enhancing alfalfa-leaf function as claimed in claim 1, it is characterised in that described
The preparation method of nutrient solution is：30-50 parts of fern root is taken, is soaked in 3-5 days in the 8-12 times of clear water of quality, then outwell water,
Fern root is put into juice extractor, the water for rejoining 3-5 times of quality is squeezed the juice, the mixture after heating is squeezed the juice extremely seethes with excitement, and carries
1-2h is taken, is then filtered, collect filtrate, when liquid temperature degree to be filtered is down to room temperature, 25mg/L NH are separately added into toward filtrate4NO3、
28mg/L KNO3、6.5mg/L CaCl2.2H2O、3.6mg/L MgSO4.7H2O、2.5mg/L KH2PO4With 6-12g/L NaCl,
After well mixed, that is, obtain described nutrient solution.
3. the breeding method of a kind of efficient enhancing alfalfa-leaf function as claimed in claim 1, it is characterised in that described
The light index in dim light area is 50-60 luxs, and the light index of highlights is 80-100 luxs.
4. the breeding method of a kind of efficient enhancing alfalfa-leaf function as claimed in claim 1, it is characterised in that described
It is concentrated liquid fertilizer that growth promotes fertilizer, and component content is：6- benzylaminopurines 45g/L, nitrogen 35g/L, phosphoric anhydride 18g/L, oxidation
Potassium 15.6g/L, magnesia 1.0g/L, sulphur 1.5g/L.
5. the breeding method of a kind of efficient enhancing alfalfa-leaf function as claimed in claim 1, it is characterised in that described
More than bacterium solution organic fertilizer N P and K total content 15wt%, more than content of organic matter 70wt%, effective total viable count 0.2 hundred million/gram with
On.
6. the breeding method of a kind of efficient enhancing alfalfa-leaf function as claimed in claim 1, it is characterised in that described
P in soil H values are 6.5-6.7.
7. the breeding method of a kind of efficient enhancing alfalfa-leaf function as claimed in claim 1, it is characterised in that described
Alfalfa is Longdong alfalfa.
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| CN107646560A (en) * | 2017-09-28 | 2018-02-02 | 中国科学院西北高原生物研究所 | The implantation methods of Alpine-arctic Pastoral alfalfa |
| CN113348805A (en) * | 2021-06-21 | 2021-09-07 | 攀枝花市农林科学研究院 | Method for improving yield and quality of greenhouse vegetables |
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