CN106856724A - A kind of germination treatment method of wax of use straw ash to beet Wild related germplasm - Google Patents
A kind of germination treatment method of wax of use straw ash to beet Wild related germplasm Download PDFInfo
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- CN106856724A CN106856724A CN201710245452.XA CN201710245452A CN106856724A CN 106856724 A CN106856724 A CN 106856724A CN 201710245452 A CN201710245452 A CN 201710245452A CN 106856724 A CN106856724 A CN 106856724A
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- beet
- straw ash
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
A kind of germination treatment method of wax of use straw ash to beet Wild related germplasm, it is related to technical field of plant propagation, the technical problem to be solved in the present invention is that beet seed speed of germination is slow, germination percentage is low, complex operation, the reagent cost for using is high, and has pollution, the problem of industrialized production is not easy to, the inventive method includes:(1) seed is gathered；(2) seed is cleaned；(3) clear water immersion seed；(4) straw ash solution cleaning seed；(5) seed after straw ash is cleaned is wrapped up with wet gauze, is placed in germination box；(6) germination box is integrally placed in constant incubator and is cultivated, seed is cleaned with straw ash solution daily during culture.By above-mentioned processing mode, beet seed can germination when 2~3 weeks.The present invention significantly shorten the germinating time of beet seed, and not only easy to operate, method is safer, free from environmental pollution, adapt to the market demand of beet breeding, with preferable actual application value.
Description
Technical field
It is more particularly to a kind of sub to beet Wild related germplasm using straw ash the present invention relates to technical field of plant propagation
Germination treatment method of wax.
Background technology
Beet (Beta vulgaris L.) is Chenopodiaceae Beta beet kind biennial herb plant, there is root garden beet, leaf
The kind such as garden beet and sugar beet, originates from one of Mediterranean, ready-made sugared section crop main for China.
Sugar is carried by the method for oozing out and carbonatation process is clarified, beet can be directly produced white sugar, additionally, beet is easy to digest, can improve
Appetite, moreover it is possible to alleviate headache, play effect of preventing cold and anaemia.Contain beet root：1. iodine, can prevent goitre
Greatly, atherosclerosis is prevented and treated；2. glycine betaine, can adjust metabolism, and help absorbs albumen and improves liver function；3. fiber
Element, pectin and trace chemical elements, anti-gastric-ulcer and alleviation abdominal distension, important work is played to diseases such as treatment hypertension and anaemias
With.
The skin of beet seed is thicker, and water suction is slow, and germination percentage is low, and speed of germination and the regularity that sprouts are poor.At present, the country is to sweet
The research of the germination technology of colza is relatively on the low side.Once scholar proposes with concentration 10g/L H2O2、0.5g/L H3BO3And 5g/
LKH2PO4Treatment beet seed is more suitable, but this method complex operation, and relatively costly, germination percentage is low.Therefore, probe into how
The germination percentage accelerated germination and improve seed is particularly significant.
And, existing seed is sprouted to use the modes such as hormone mostly and coordinate and promotes seed to sprout in modes such as nutriments
Hair, improves germination percentage, and the most high cost of this mode, not environmentally, and cumbersome is not easy to industrialized production.To how
Improving the germination rate of beet seed, reduce environmental pollution, industrialized production does not have reference, therefore be badly in need of one kind now has
The beet seed germination method that effect solves the above problems.
The content of the invention
The technical problem to be solved in the present invention is to provide at a kind of germination sub to beet Wild related germplasm of use straw ash
Reason method, can solve the problem that beet seed germination speed is slow, and bud ratio is low, complex operation, and the reagent cost for using is high, and has dirt
Dye, is not easy to the problem of industrialized production.
To the germination treatment method of wax of beet Wild related germplasm, it is according to following step to a kind of use straw ash of the invention
Suddenly carry out：
First, collection beet Wild related germplasm, clear water rinses seed；
2nd, soak beet seed 10~14 hours using clear water, then clean seed 1~2 time with straw ash solution；
3rd, in three layers of wet gauze of germination cassette bottom portion tiling, the beet seed that step 2 is treated is placed on wet gauze, then
Three layers of wet gauze of lid on beet seed, then will be integrally placed in constant incubator continuous culture 2~3 weeks, that is, complete described in
Use straw ash to beet Wild related germplasm germination treatment；Wherein, straw ash solution concentration is 30~70g/L.
The present invention includes following beneficial effect：
Low cost of the present invention, it is more easy to operate, seed is cleaned only with straw ash solution, just can effectively improve beet seed
Germination percentage (up to more than 95%), shortens germinating time (only needing 2-3 weeks).The invention provides a kind of environmental protection and it is easy to industry
Change the technology of big production, greatly save production cost, with great application value, promote with existing use various reagents
Sprouting is compared, and the method for the present invention has responded the call of twice laid, and environment is not polluted, and decreases the cumbersome of operation
Property, it is a kind of technology of great popularization.
Specific embodiment
Specific embodiment one：A kind of germination treatment of the use straw ash of present embodiment to beet Wild related germplasm
Method, it is followed the steps below：
First, collection beet Wild related germplasm, clear water rinses seed；
2nd, soak beet seed 10~14 hours using clear water, then clean seed 1~2 time with straw ash solution；
3rd, in three layers of wet gauze of germination cassette bottom portion tiling, the beet seed that step 2 is treated is placed on wet gauze, then
Three layers of wet gauze of lid on beet seed, then will be integrally placed in constant incubator continuous culture 2~3 weeks, that is, complete described in
Use straw ash to beet Wild related germplasm germination treatment；Wherein, straw ash solution concentration is 30~70g/L.
Specific embodiment two：Present embodiment and specific embodiment one：Described straw ash solution was by burning
Stalk is obtained, i.e., to addition clear water stirring 20min, the as supernatant obtained after filtration residue, straw ash solution in straw ash.
Other are identical with specific embodiment one.
Specific embodiment three：Present embodiment and specific embodiment one：Described stalk is maize straw.Other with
Specific embodiment one is identical.
Specific embodiment four：Present embodiment and specific embodiment one：Clear water temperature described in step one and two is
15~20 DEG C.Other are identical with specific embodiment one.
Specific embodiment five：Present embodiment and specific embodiment one：Clear water temperature described in step one and two is
18~20 DEG C.Other are identical with specific embodiment one.
Specific embodiment six：Present embodiment and specific embodiment one：Straw ash solution concentration is 30~50g/L.
Other are identical with specific embodiment one.
Specific embodiment seven：Present embodiment and specific embodiment one：Straw ash solution concentration is 50~70g/L.
Other are identical with specific embodiment one.
Specific embodiment eight：Present embodiment and specific embodiment one：Straw ash solution concentration is 40~60g/L.
Other are identical with specific embodiment one.
Specific embodiment nine：Present embodiment and specific embodiment one：Constant incubator temperature is 22~25 DEG C, and
Clean wet gauze need to be changed once daily in the training period, and 1~2 beet seed is rinsed with straw ash solution.Other with
Specific embodiment one is identical.
Specific embodiment ten：Present embodiment and specific embodiment one：The humidity of described wet gauze is that can not twist
Water is advisable.Other are identical with specific embodiment one.
Present invention is not limited only to the content of the respective embodiments described above, the group of one of them or several specific embodiments
Contract sample can also realize the purpose of invention.
Beneficial effects of the present invention are verified by following examples：
Example 1
To the germination treatment method of wax of beet Wild related germplasm, it is according to following to a kind of use straw ash of the present embodiment
What step was carried out：
(1) selected full beet Wild related germplasm 50, after being rinsed well through clear water, 15 DEG C is immersed in by seed
12h in running water；
(2) by the maize straw ash for burning：Running water=500g：After the formula of 10L is converted, stirred very using glass bar
Clock, the supernatant for filtering as stalk buck, using the seed after stalk buck cleaning and dipping once；
(3) (wet gauze is twisted not water outlet and is advisable with hand, and gauze and germination box need to be in advance in germination cassette bottom layer to spread three layers of wet gauze
Sterilize 20min at 121 DEG C), then by the evenly spaced row of seed after immersion on wet gauze, lid three again on seed
Layer wet gauze；
(4) the above-mentioned germination box for completing wet gauze and seed is put in constant incubator, incubated at 22 DEG C, training
Seed is cleaned during supporting once using stalk buck daily.The quantity of chitting piece is counted respectively when cultivating to 2 weeks, 3 weeks, and is counted
Germination percentage is calculated, 1 is the results are shown in Table.
Example 2
To the germination treatment method of wax of beet Wild related germplasm, it is according to following to a kind of use straw ash of the present embodiment
What step was carried out：
(1) selected full beet Wild related germplasm 50, after being rinsed well through clear water, 20 DEG C is immersed in by seed
12h in running water；
(2) by the maize straw ash for burning：Running water=500g：After the formula of 10L is converted, stirred very using glass bar
Clock, the supernatant for filtering as stalk buck, using the seed after stalk buck cleaning and dipping once；
(3) (wet gauze is twisted not water outlet and is advisable with hand, and gauze and germination box need to be in advance in germination cassette bottom layer to spread three layers of wet gauze
Sterilize 20min at 121 DEG C), then by the evenly spaced row of seed after immersion on wet gauze, lid three again on seed
Layer wet gauze；
(4) the above-mentioned germination box for completing wet gauze and seed is put in constant incubator, incubated at 25 DEG C, training
Seed is cleaned during supporting once using stalk buck daily.The quantity of chitting piece is counted respectively when cultivating to 2 weeks, 3 weeks, and is counted
Germination percentage is calculated, 1 is the results are shown in Table.
Sprouting of the different disposal condition of table 1 on beet seed influences
| 2 weeks | 3 weeks | |
| The percentage of seedgermination of example 1 | 82% | 98% |
| The percentage of seedgermination of example 2 | 84% | 96% |
The beet seed of embodiment 1 and 2 germinateed substantially at 2~3 weeks, effect is significant, it follows that using embodiment 1
Method with 2 sprouts the cycle to shortening beet seed, with effect well.
Embodiment 3
To the germination treatment method of wax of beet Wild related germplasm, it is according to following to a kind of use straw ash of the present embodiment
What step was carried out：
(1) selected full beet Wild related germplasm 50, after being rinsed well through clear water, 18 DEG C is immersed in by seed
12h in running water；
(2) by the maize straw ash for burning：Running water=300g：After the formula of 10L is converted, stirred very using glass bar
Clock, the supernatant for filtering as stalk buck, using the seed after stalk buck cleaning and dipping once；
(3) (wet gauze is twisted not water outlet and is advisable with hand, and gauze and germination box need to be in advance in germination cassette bottom layer to spread three layers of wet gauze
Sterilize 20min at 121 DEG C), then by the evenly spaced row of seed after immersion on wet gauze, lid three again on seed
Layer wet gauze；
(4) the above-mentioned germination box for completing wet gauze and seed is put in constant incubator, incubated at 25 DEG C, training
Seed is cleaned during supporting once using stalk buck daily.
Embodiment 4
To the germination treatment method of wax of beet Wild related germplasm, it is according to following to a kind of use straw ash of the present embodiment
What step was carried out：
(1) selected full beet Wild related germplasm 50, after being rinsed well through clear water, 20 DEG C is immersed in by seed
12h in running water；
(2) by the maize straw ash for burning：Running water=700g：After the formula of 10L is converted, stirred very using glass bar
Clock, the supernatant for filtering as stalk buck, using the seed after stalk buck cleaning and dipping once；
(3) (wet gauze is twisted not water outlet and is advisable with hand, and gauze and germination box need to be in advance in germination cassette bottom layer to spread three layers of wet gauze
Sterilize 20min at 121 DEG C), then by the evenly spaced row of seed after immersion on wet gauze, lid three again on seed
Layer wet gauze；
(4) the above-mentioned germination box for completing wet gauze and seed is put in constant incubator, incubated at 25 DEG C, training
Seed is cleaned during supporting once using stalk buck daily.
Embodiment 5
To the germination treatment method of wax of beet Wild related germplasm, it is according to following to a kind of use straw ash of the present embodiment
What step was carried out：
(1) selected full beet Wild related germplasm 50, after being rinsed well through clear water, 15 DEG C is immersed in by seed
12h in running water；
(2) by the maize straw ash for burning：Running water=600g：After the formula of 10L is converted, stirred very using glass bar
Clock, the supernatant for filtering as stalk buck, using the seed after stalk buck cleaning and dipping once；
(3) (wet gauze is twisted not water outlet and is advisable with hand, and gauze and germination box need to be in advance in germination cassette bottom layer to spread three layers of wet gauze
Sterilize 20min at 121 DEG C), then by the evenly spaced row of seed after immersion on wet gauze, lid three again on seed
Layer wet gauze；
(4) the above-mentioned germination box for completing wet gauze and seed is put in constant incubator, incubated at 25 DEG C, training
Seed is cleaned during supporting once using stalk buck daily.
Embodiment 6
To the germination treatment method of wax of beet Wild related germplasm, it is according to following to a kind of use straw ash of the present embodiment
What step was carried out：
(1) selected full beet Wild related germplasm 50, after being rinsed well through clear water, 20 DEG C is immersed in by seed
12h in running water；
(2) by the maize straw ash for burning：Running water=400g：After the formula of 10L is converted, stirred very using glass bar
Clock, the supernatant for filtering as stalk buck, using the seed after stalk buck cleaning and dipping once；
(3) (wet gauze is twisted not water outlet and is advisable with hand, and gauze and germination box need to be in advance in germination cassette bottom layer to spread three layers of wet gauze
Sterilize 20min at 121 DEG C), then by the evenly spaced row of seed after immersion on wet gauze, lid three again on seed
Layer wet gauze；
(4) the above-mentioned germination box for completing wet gauze and seed is put in constant incubator, incubated at 25 DEG C, training
Seed is cleaned during supporting once using stalk buck daily.
The germination rate of the beet seed after the treatment of embodiment 3 to 6 can reach 90% or so after 3 weeks, in 2~3 weeks bases
This is all sprouted.
By above example, the method for the present invention just can be provided only with straw ash and improve beet kind well
Sub- germination rate, for industrialized production provides basis.And the straw ash of present invention use processes the condition of beet seed method
Optimization is not limited to the content cited by above example, and any mode for not departing from inventive concept can reach of the invention
Purpose, belongs to protection scope of the present invention.
General principle of the invention and operating process are the foregoing described, the present invention is not limited to the above embodiments, it is above-mentioned
Simply general principle of the invention described in embodiment and specification.The claimed scope of the invention is by appended claim
Book and its equivalent thereof.
Claims (10)
1. a kind of use straw ash to beet Wild related germplasm germination treatment method of wax, it is characterised in that it is according to following step
Suddenly carry out：
First, collection beet Wild related germplasm, clear water rinses seed；
2nd, soak beet seed 10~14 hours using clear water, then clean seed 1~2 time with straw ash solution；
3rd, in three layers of wet gauze of germination cassette bottom portion tiling, the beet seed that step 2 is treated is placed on wet gauze, then sweet
The upper three layers of wet gauze of lid of colza, then will be integrally placed at continuous culture 2~3 weeks in constant incubator, that is, complete described in adopting
With the straw ash germination treatment sub to beet Wild related germplasm；Wherein, straw ash solution concentration is 30~70g/L.
2. a kind of use straw ash according to claim 1 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
It is that stalk of the described straw ash solution by burning is obtained to levy, i.e., to clear water stirring 20min is added in straw ash, filter residual
The supernatant obtained after slag, as straw ash solution.
3. a kind of use straw ash according to claim 1 and 2 to beet Wild related germplasm germination treatment method of wax, its
It is characterised by that described stalk is maize straw.
4. a kind of use straw ash according to claim 1 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
Levy is that clear water temperature described in step one and two is 15~20 DEG C.
5. a kind of use straw ash according to claim 1 or 4 to beet Wild related germplasm germination treatment method of wax, its
It is characterised by that the clear water temperature described in step one and two is 18~20 DEG C.
6. a kind of use straw ash according to claim 1 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
Levy is that straw ash solution concentration is 30~50g/L.
7. a kind of use straw ash according to claim 6 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
Levy is that straw ash solution concentration is 50~70g/L.
8. a kind of use straw ash according to claim 7 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
Levy is that straw ash solution concentration is 40~60g/L.
9. a kind of use straw ash according to claim 1 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
It is that constant incubator temperature is 22~25 DEG C to levy, and need to change once clean wet gauze daily in the training period, and uses straw
Stalk ash solution rinses 1~2 beet seed.
10. a kind of use straw ash according to claim 1 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
The humidity for levying the wet gauze described in being is to twist not water outlet to be advisable.
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| CN201710245452.XA CN106856724A (en) | 2017-04-14 | 2017-04-14 | A kind of germination treatment method of wax of use straw ash to beet Wild related germplasm |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111066590A (en) * | 2019-12-30 | 2020-04-28 | 四川辉氏生物技术有限公司 | Method for planting ricepaperplant pith |
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| CN103621219A (en) * | 2013-12-18 | 2014-03-12 | 陇西县效德中药材有限责任公司 | Method for processing scutellaria seeds |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111066590A (en) * | 2019-12-30 | 2020-04-28 | 四川辉氏生物技术有限公司 | Method for planting ricepaperplant pith |
| CN111066590B (en) * | 2019-12-30 | 2022-03-18 | 四川辉氏生物技术有限公司 | Method for planting ricepaperplant pith |
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