CN106856716A - A kind of golden larch seed pre-sowing treatment method - Google Patents
A kind of golden larch seed pre-sowing treatment method Download PDFInfo
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- CN106856716A CN106856716A CN201710119405.0A CN201710119405A CN106856716A CN 106856716 A CN106856716 A CN 106856716A CN 201710119405 A CN201710119405 A CN 201710119405A CN 106856716 A CN106856716 A CN 106856716A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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Abstract
The invention discloses a kind of golden larch seed pre-sowing treatment method, step is as follows：（1）Golden larch seed sterilizing；（2）Golden larch seed activity agent immersion treatment；（3）N+Ion beam mutation golden larch seed；（4）The low temperature drying of golden larch seed is preserved, nursery after ultrasonication.The present invention passes through chemicals treatment, N+The cooperative effect of ion beam mutation and ultrasonication, emergence rate is all substantially increased to golden larch novel species and old kind, and protection and utilization, golden larch production and the raising of economic benefit of the present invention to golden larch germ plasm resource are respectively provided with significance.
Description
Technical field
The present invention relates to ornamental plant science and technology field, specifically a kind of golden larch seed pre-sowing treatment method.
Background technology
Pseudolarix Pinaceae fallen leaves abieteae Pseudolarix, single category single, is Chinese distinctive Relict Plant, earliest change
Stone is found in geochronic Late Cretaceous strata, and the Palaeocene Epoch to the Pliocene Epoch is in Spitsbergen, Europe, U.S.
Continent, Asia are also found.Speculated according to paleo information, golden larch may originate from Cretaceous period middle and advanced stage, once be distributed widely in north
Between 33 °~52 ° of latitude；Once North China of China was distributed in before Middle Pleistocene；After Great Ice Age Pleistocene, various regions golden larch is successive
Extinction, only survives in China In Middle And Lower Reaches of Changjiang River, as distinctive single category single plant Chinese now.According to the world
Conservation of nature alliance（IUCN）The standard of the compromised categorizing system standard of the new species formulated and Chinese Plants Red Data Book, money
Song Jun is decided to be vulnerable species, and is listed in Chinese Second Class Key Protected Plant.
Money pine trunk is logical straight, tree crown ovate turriform, magnificent attractive in appearance.Leafage life is in the form of annular discs on brachyplast, and leaf color is initially tender
Green, autumn has set in switchs to golden yellow, therefore named golden larch, is the commerical tree species such as excellent building, furniture and Landscape Trees, in history
Cultivated as precious commerical tree species and ornamental tree species.Golden larch has ornamental value higher, its tree-like grace, blade bar
Shape, flat, flexible, spring and summer is light green color, and soft kingfisher is tender and lovely, becomes golden yellow after autumn, and circle such as copper coin, such as cluster cluster gold, are excellent sights
Reward seeds.Early in 19th century just by Introduction of American, at present in cultivation extensively all over the world, with araucaria, deodar, day capital
Pine, North America big tree are collectively referred to as the big flower garden trees in the world five.Golden larch is precious timber Trees, and its trunk is logical straight, and material is excellent,
Can be used as to build, furniture and the wood-fibred raw material of industry etc.；Blade can extract plant volatile oil, be applied to pharmacy, spices, essence,
The industry such as cosmetics and food；Root skin is traditional Chinese medicine, is commonly called as corter pseudolaricis, and energy desinsection is antipruritic, antimycotic, external curing hand
The ringworm of the foot, neurodermatitis, eczema etc..Research finds that the chemical composition of corter pseudolaricis mainly has Diterpenes, triterpenes, triterpene lactone
And sterols and other class compounds.Modern pharmacology experiment shows that the natural extract of golden larch has antimycotic, anti-swelling
The effects such as knurl, antifertility, suppression angiogenesis.
Although having research at present carries out the modess of reproduction such as cuttage, grafting, the tissue culture of golden larch, research rank is only rested on
Section, technology is not mature enough, does not have application value.Seminal propagation is the main modes of reproduction of golden larch under nature, golden larch
Seed seedling-raising is the necessary link of production.But there is dividing for biennial bearing because golden larch is solid, could get bumper crops once within 3~5 years, and
Seed is rich in grease, and kind shell is very thin, therefore novel species bud ratio is not high, and general novel species bud ratio is all below 40%, and warp is every two
Seed is aged caused by various factors after 3 years storages.Old kind of viability of golden larch declines, emergence rate is more reduced, to golden larch
Nursery production bring larger economic loss.Therefore, how to improve the vigor of golden larch seed, to the protection of germ plasm resource and
It is significant using the production of, golden larch and the raising of economic benefit.
The content of the invention
The purpose of the present invention is directed to the existing defect that golden larch seed vitality is poor, bud ratio is low, there is provided a kind of
By chemicals treatment, N+The cooperative effect of ion beam mutation and ultrasonication, so as to substantially increase golden larch novel species and old
The method for planting viability and emergence rate.
The purpose of the present invention is solved by the following technical programs：
A kind of golden larch seed pre-sowing treatment method, it is characterised in that：The step of golden larch seed pre-sowing treatment method is：
（1）Golden larch seed sterilizing：To be put into culture dish after pending golden larch wash seeds, added in culture dish
Enter 0.6% liquor natrii hypochloritis, sterilize 15~20min, ultrapure water is used 3~5 times after sterilizing；
（2）Golden larch seed activity agent immersion treatment：Golden larch seed is first soaked into 2~4h in lower concentrations of active agent, then
Seed is taken out, is dried, sprayed the activating agent of high concentration with watering can and stirring golden larch seed makes seed uniformly soak, dried in the air again
It is dry, repeatedly by baked seed after 2~3 times；
（3）N+Ion beam mutation golden larch seed：Golden larch seed embryo portion is put into sample disc towards ion beam mutation direction, from
Son injection before start preheating 2h, under vacuo with energy as 25KeV, line size 10mA, each burst length as 5s, target chamber it is true
Reciprocal of duty cycle is 6.0 × 10-4~1.3 × 10-3Pa carries out N+Ion beam mutation；
（4）The low temperature drying of golden larch seed is preserved, nursery after ultrasonication：By the golden larch seed low temperature after ion beam mutation
Kept dry, with 20% polyglycol solution seed soaking 24h before nursery, then with 55~65kHz ultrasonications 15min, Ran Houqu
Go out, seeding and seedling raising after 2~3d of presoaking and germinating.
The step（2）Middle lower concentrations of active agent component and proportioning are：ZnSO420~25g/L, CuSO4·5H2O 10~
12g/L, MnCl26~8g/L；Concentration of component is 12~15 times of lower concentrations of active agent in the high concentration activating agent.
The step（3）Middle N+Ion beam mutation dosage is 6~8 × 1036ion/cm2。
The step（4）Middle low temperature drying is preserved and refers to temperature less than 5 DEG C, and humidity is less than 45%.
The present invention has the following advantages compared to existing technology：
Although having N at present+The research that ion beam mutation and ultrasonication vegetable seeds can improve bud ratio is reported, but N+From
Beamlet injects very limited to the facilitation effect that seed is sprouted with ultrasonication, and practical value is relatively low.The invention
The method for applying ion beam mutation and ultrasonication after being processed using activating agent again, chemicals treatment, N+Ion beam mutation and super
Occurs significant cooperative effect after sonicated, so as to substantially increase the viability and emergence rate of golden larch seed, to money
Protection and utilization, golden larch production and the raising of economic benefit of loose germ plasm resource are respectively provided with significance.
Specific embodiment
With reference to embodiment, the present invention is further illustrated.
A kind of golden larch seed pre-sowing treatment method, it is characterised in that：The step of the golden larch seed pre-sowing treatment method
Suddenly it is：
（1）Golden larch seed sterilizing：To be put into culture dish after pending golden larch wash seeds, added in culture dish
Enter 0.6% liquor natrii hypochloritis, sterilize 15~20min, ultrapure water is used 3~5 times after sterilizing；
（2）Golden larch seed activity agent immersion treatment：By golden larch seed first in lower concentrations of active agent（Lower concentrations of active agent component
And proportioning is：ZnSO420~25g/L, CuSO4·5H2O 10~12g/L, MnCl26~8g/L）2~4h of middle immersion, then
Seed is taken out, is dried, the activating agent of high concentration is sprayed with watering can（Concentration of component is lower concentrations of active agent in high concentration activating agent
12~15 times）And stirring golden larch seed makes seed uniformly soak, and dries again, repeatedly by baked seed after 2~3 times；
（3）N+Ion beam mutation golden larch seed：Golden larch seed embryo portion is put into sample disc towards ion beam mutation direction, from
Son injection before start preheating 2h, under vacuo with energy as 25KeV, line size 10mA, each burst length as 5s, target chamber it is true
Reciprocal of duty cycle is 6.0 × 10-4~1.3 × 10-3Pa carries out N+Ion beam mutation, N+Ion beam mutation dosage is 6~8 × 1036ion/
cm2；
（4）The low temperature drying of golden larch seed is preserved, nursery after ultrasonication：Golden larch seed after ion beam mutation is placed in
Temperature is less than under 45% low temperature drying environment less than 5 DEG C, humidity and preserves, with 20% polyglycol solution seed soaking 24h before nursery, so
Afterwards with 55~65kHz ultrasonication 15min, then take out, seeding and seedling raising after 2~3d of presoaking and germinating.
Embodiment
First, influence of the different disposal method to golden larch seed seedling-raising survival rate
The new golden larch seed for harvesting being purchased from the market within 2013, germination percentage test being carried out then, obtaining novel species germination percentage is
35.8%, temperature is subsequently placed in less than being preserved under 5 DEG C, low temperature drying environment of the humidity less than 45%, in March, 2015 newly buys money
Loose seed, and take out old kind and carry out germination test, the average germination percentage of the old kind of golden larch is 12.1%, it can be found that golden larch is old
The bud ratio planted is lower.
Then March take golden larch novel species and old kind respectively, be respectively classified into 8 groups, novel species group and old kind group every group 160 are educated
Before seedling is respectively adopted carries out different disposal method, then detects percentage of seedgermination change.The corresponding processing method of different grouping is such as
Under：
1st group of novel species group and old kind group use integrated conduct method of the invention, specially：（1）Golden larch seed sterilization disappears
Poison：To be put into culture dish after pending golden larch wash seeds, 0.6% liquor natrii hypochloritis is added in culture dish, sterilized
17.5min, ultrapure water is used 4 times after sterilizing；（2）Golden larch seed activity agent immersion treatment：By golden larch seed first low
Concentration activating agent（Lower concentrations of active agent component and proportioning are：ZnSO422.5g/L, CuSO4·5H2O 11g/L, MnCl2 7g/
L）Middle immersion 3h, then takes out seed, dries, and the activating agent of high concentration is sprayed with watering can（Component is dense in high concentration activating agent
Spend 12 times for lower concentrations of active agent）And stirring golden larch seed makes seed uniformly soak, and dries again, will plant after 2 times repeatedly
Son drying；（3）N+Ion beam mutation golden larch seed：Golden larch seed embryo portion is put into sample towards ion beam mutation direction
Disk, before ion implanting start shooting preheating 2h, under vacuo with energy as 25KeV, line size 10mA, each burst length as 5s,
Target chamber vacuum is 8.5~9.5 × 10-4Pa carries out N+Ion beam mutation, N+Ion beam mutation dosage is 7 × 1036ion/cm2；
（4）The low temperature drying of golden larch seed is preserved, nursery after ultrasonication：Golden larch seed after ion beam mutation is placed in temperature
Kept dry in 0 degree of Low temperature refrigerator, is soaked seed 24h, then with 60kHz ultrasonic waves before nursery with 20% polyglycol solution
Reason 15min, then takes out, seeding and seedling raising after presoaking and germinating 3d, surveys germination percentage；
2nd group of novel species group and old kind group only with chemicals treatment, specially：（1）Golden larch seed sterilizing：Will be pending
Golden larch wash seeds after be put into culture dish, add 0.6% liquor natrii hypochloritis in culture dish, sterilize 17.5min, goes out
Ultrapure water is used after bacterium 4 times；（2）Golden larch seed activity agent immersion treatment：By golden larch seed first in lower concentrations of active agent
（Lower concentrations of active agent component and proportioning are：ZnSO422.5g/L, CuSO4·5H2O 11g/L, MnCl27g/L）Middle immersion 3h,
Then seed is taken out, is dried, the activating agent of high concentration is sprayed with watering can（Concentration of component is low concentration work in high concentration activating agent
12 times of property agent）And stirring golden larch seed makes seed uniformly soak, and dries again, will be sowed after baked seed after 2 times repeatedly
Nursery, surveys germination percentage；
3rd group of novel species group and old kind group are only with N+Ion beam mutation treatment, specially：（1）Golden larch seed sterilizing：
To be put into culture dish after pending golden larch wash seeds, 0.6% liquor natrii hypochloritis is added in culture dish, sterilized
17.5min, ultrapure water is used 4 times after sterilizing；（2）N+Ion beam mutation golden larch seed：By embryo after golden larch baked seed
Portion is put into sample disc towards ion beam mutation direction, before ion implanting start shooting preheating 2h, under vacuo with energy as 25KeV, beam
Stream size 10mA, each burst length are 5s, target chamber vacuum is 8.5~9.5 × 10-4Pa carries out N+Ion beam mutation, N+From
Beamlet implantation dosage is 7 × 1036ion/cm2, then nursery, survey germination percentage；
4th group of novel species group and old kind group only with ultrasonication, specially：（1）Golden larch seed sterilizing：To wait to locate
It is put into culture dish after the golden larch wash seeds of reason, 0.6% liquor natrii hypochloritis is added in culture dish, sterilizes 17.5min,
Ultrapure water is used after sterilizing 4 times；（2）The low temperature drying of golden larch seed is preserved, nursery after ultrasonication：After drying and processing
Golden larch seed be placed in kept dry in the Low temperature refrigerator of 0 degree of temperature, before nursery with 20% polyglycol solution soak seed 24h,
Then 60kHz ultrasonication 15min are used, is then taken out, seeding and seedling raising after presoaking and germinating 3d, survey germination percentage；
5th group of novel species group and old kind group are only with chemicals treatment and N+Ion beam mutation treatment, specially：（1）Golden larch kind
Sub- sterilizing：To be put into culture dish after pending golden larch wash seeds, 0.6% sodium hypochlorite is added in culture dish
Solution, sterilized 17.5min, and ultrapure water is used 4 times after sterilizing；（2）Golden larch seed activity agent immersion treatment：By golden larch kind
It is sub first in lower concentrations of active agent（Lower concentrations of active agent component and proportioning are：ZnSO422.5g/L, CuSO4·5H2O 11g/L,
MnCl27g/L）Middle immersion 3h, then takes out seed, dries, and the activating agent of high concentration is sprayed with watering can（High concentration activating agent
Interior concentration of component is 12 times of lower concentrations of active agent）And stirring golden larch seed makes seed uniformly soak, and dries again, repeatedly 2
By baked seed after secondary；（3）N+Ion beam mutation golden larch seed：Golden larch seed embryo portion is put towards ion beam mutation direction
Enter sample disc, before ion implanting start shooting preheating 2h, under vacuo with energy as 25KeV, line size 10mA, each burst length
For 5s, target chamber vacuum are 8.5~9.5 × 10-4Pa carries out N+Ion beam mutation, N+Ion beam mutation dosage is 7 × 1036ion/
cm2；Seeding and seedling raising after presoaking and germinating 3d, surveys germination percentage；
6th group of novel species group and old kind group only with chemicals treatment and ultrasonication, specially：（1）Golden larch seed is sterilized
Sterilization：To be put into culture dish after pending golden larch wash seeds, 0.6% liquor natrii hypochloritis is added in culture dish, gone out
Bacterium 17.5min, ultrapure water is used 4 times after sterilizing；（2）Golden larch seed activity agent immersion treatment：Golden larch seed is first existed
Lower concentrations of active agent（Lower concentrations of active agent component and proportioning are：ZnSO422.5g/L, CuSO4·5H2O 11g/L, MnCl2
7g/L）Middle immersion 3h, then takes out seed, dries, and the activating agent of high concentration is sprayed with watering can（Component in high concentration activating agent
Concentration is 12 times of lower concentrations of active agent）And stirring golden larch seed makes seed uniformly soak, and dries again, repeatedly will after 2 times
Baked seed；（3）The low temperature drying of golden larch seed is preserved, nursery after ultrasonication：Golden larch seed is placed in 0 degree of temperature
Kept dry in Low temperature refrigerator, with 20% polyglycol solution seed soaking 24h before nursery, then uses 60kHz ultrasonications
15min, then takes out, seeding and seedling raising after presoaking and germinating 3d, surveys germination percentage；
7th group of novel species group and old kind group are only with N+Ion beam mutation treatment and ultrasonication, specially：（1）Golden larch
Seed sterilizing：To be put into culture dish after pending golden larch wash seeds, 0.6% hypochlorous acid is added in culture dish
Sodium solution, sterilized 17.5min, and ultrapure water is used 4 times after sterilizing；（2）N+Ion beam mutation golden larch seed：By golden larch kind
Embryo portion is put into sample disc towards ion beam mutation direction after son drying, and start shooting preheating 2h before ion implanting, under vacuo with energy
For 25KeV, line size 10mA, each burst length be 5s, target chamber vacuum be 8.5~9.5 × 10-4Pa carries out N+Ion beam
Injection, N+Ion beam mutation dosage is 7 × 1036ion/cm2；（3）The low temperature drying of golden larch seed is preserved, and is educated after ultrasonication
Seedling：Golden larch seed after ion beam mutation is placed in kept dry in the Low temperature refrigerator of 0 degree of temperature, it is poly- with 20% before nursery
Ethylene glycol solution seed soaking 24h, then with 60kHz ultrasonication 15min, then takes out, seeding and seedling raising after presoaking and germinating 3d, surveys
Germination percentage；
8th group of novel species group and old kind group are left intact, and for blank, survey the germination of golden larch novel species and old kind
Rate.The germination percentage test result of golden larch seed is as shown in table 1 below under Different treatments.
By the Different treatments of table 1 on golden larch novel species and the old kind of result of the test of germination percentage influence it can be found that medicament
Treatment, N+Ion beam mutation and ultrasonication can all produce positive result to golden larch novel species and old kind of germination percentage, but
Single treatment（2nd, 3,4 groups）Influence to germination percentage is smaller（Novel species germination percentage distinguishes gain 2%, 4.3%, 2.2%；It is old to plant hair
Bud rate distinguishes gain 0.8%, 5.7%, 2.8%；）.Three kinds of modes process the old kind of golden larch simultaneously（1st group）, the hair of the old kind of golden larch
Bud rate is obviously improved, and novel species and old kind of germination percentage have respectively reached 49.8% and 29.7%, and germination percentage increased respectively
14.9% and 17.6%, and three kinds of independent enhancement effect sums for applying for the treatment of are respectively 8.5% and 9.3%, illustrate three kinds for the treatment of sides
The common applying of formula generates synergy.
Analytical table 1 is it is also found that removal chemicals treatment or N+The dual factors test of ion beam mutation（6th, 7 groups）, knot
Fruit golden larch germination percentage is significantly reduced, and the germination percentage that germination percentage is processed with single factor test relatively, and removes ultrasonication
Processing mode（5th group）Although, the germination percentage reduction of the old kind of golden larch, close to the effect that three factors apply jointly, explanation
Synergy is mainly chemicals treatment and N+What ion beam mutation interaction was produced.
2nd, chemicals treatment and N+Ion beam mutation interaction improves the Analysis on Mechanism of golden larch germination percentage
Ion beam mutation produces the differences such as biological effect and radiation, and it is Trinitarian that ion beam plays a role, including energy is heavy
Product, quality deposition and charge-exchange.Usual condition, ion beam mutation can have depression effect to the sprouting of vegetable seeds, but
Under low dosage situation, ion beam mutation can improve the germination percentage of seed, and this is because low energy ion beam injection can be careful to planting
Hole is caused on cell wall cell membrane, so as to improve the result of the permeability of seed., it is necessary to beat after the new seed results of golden larch
Broken dormancy could sprout, therefore, the reason that novel species germination percentage is improved is likely to ion beam mutation, and to improve seed penetrating
Property, so as to break the result of seed dormancy.
Sputtering effect of the ion beam to organism can destroy cell membrane and membrane structure, be that allogenic material is carried into cell
For good microchannel；The elecrtonegativity of cell surface can be reduced along with the quality deposition of ion implanting, acceptor is reduced external
The electrostatic repulsion of source material；Low energy ion beam causes that medicament is sputtered into cell to the direct effect and indirectly-acting of organism
In, the medicament for being deposited on seed cell surface is attacked by ion beam, so as to sputter into cell.Chemicals treatment is noted with ion beam
Enter interaction Synergistic Mechanisms has difference again, and we are had found in studying, and medicament effect can be reduced using other medicaments or other proportionings,
Select other metal ions or even depression effect can be produced, chemistry is generated in illustrating element in medicament in the golden larch seed
Effect.
In old kind of nursery of golden larch, it has been found that only ZnSO4 、CuSO4And MnCl2Combination and ion beam mutation
There is cooperative effect, wherein, zinc is relevant with the conversion of carbohydrate, can also promote the synthesis of protein, and copper is to activation of amino acid
And protein synthesis has facilitation, and manganese can promote amino acid synthetic peptide, be conducive to protein to synthesize, and can also promote hydrolase polypeptide
Generation amino acid, and transport the tissue and organ of new life to, and as ZnSO4 、CuSO4And MnCl2How to be sent out after sputtering seed
Effect is waved, its specific mechanism of action need further research.
The present invention passes through chemicals treatment, N+The cooperative effect of ion beam mutation and ultrasonication, to golden larch novel species and
Old kind all substantially increases emergence rate, and the present invention is produced and economic effect to the protection and utilization of golden larch germ plasm resource, golden larch
The raising of benefit is respectively provided with significance.
Above example is only explanation technological thought of the invention, it is impossible to limit protection scope of the present invention with this, every
According to technological thought proposed by the present invention, any change done on the basis of technical scheme each falls within the scope of the present invention
Within；The technology that the present invention is not directed to can be realized by prior art.
Claims (4)
1. a kind of golden larch seed pre-sowing treatment method, it is characterised in that：The step of golden larch seed pre-sowing treatment method
For：
（1）Golden larch seed sterilizing：To be put into culture dish after pending golden larch wash seeds, added in culture dish
Enter 0.6% liquor natrii hypochloritis, sterilize 15~20min, ultrapure water is used 3~5 times after sterilizing；
（2）Golden larch seed activity agent immersion treatment：Golden larch seed is first soaked into 2~4h in lower concentrations of active agent, then
Seed is taken out, is dried, sprayed the activating agent of high concentration with watering can and stirring golden larch seed makes seed uniformly soak, dried in the air again
It is dry, repeatedly by baked seed after 2~3 times；
（3）N+Ion beam mutation golden larch seed：Golden larch seed embryo portion is put into sample disc towards ion beam mutation direction, from
Son injection before start preheating 2h, under vacuo with energy as 25KeV, line size 10mA, each burst length as 5s, target chamber it is true
Reciprocal of duty cycle is 6.0 × 10-4~1.3 × 10-3Pa carries out N+Ion beam mutation；
（4）The low temperature drying of golden larch seed is preserved, nursery after ultrasonication：By the golden larch seed low temperature after ion beam mutation
Kept dry, with 20% polyglycol solution seed soaking 24h before nursery, then with 55~65kHz ultrasonications 15min, Ran Houqu
Go out, seeding and seedling raising after 2~3d of presoaking and germinating.
2. golden larch seed pre-sowing treatment method according to claim 1, it is characterised in that：The step（2）In it is low dense
Degree active agent component and proportioning are：ZnSO420~25g/L, CuSO4·5H2O 10~12g/L, MnCl26~8g/L；It is described
Concentration of component is 12~15 times of lower concentrations of active agent in high concentration activating agent.
3. golden larch seed pre-sowing treatment method according to claim 1, it is characterised in that：The step（3）Middle N+Ion
Beam implantation dosage is 6~8 × 1036ion/cm2。
4. golden larch seed pre-sowing treatment method according to claim 1, it is characterised in that：The step（4）Middle low temperature
Kept dry refers to temperature less than 5 DEG C, and humidity is less than 45%.
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| CN109168414A (en) * | 2018-10-29 | 2019-01-11 | 宜宾云辰乔木园林有限责任公司 | One kind promoting golden larch seed germination method |
| CN110786209A (en) * | 2019-11-28 | 2020-02-14 | 河南中医药大学 | Cultivation method for improving survival rate of selfheal seedlings and yield of selfheal |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109168414A (en) * | 2018-10-29 | 2019-01-11 | 宜宾云辰乔木园林有限责任公司 | One kind promoting golden larch seed germination method |
| CN109168414B (en) * | 2018-10-29 | 2021-11-09 | 四川云辰园林科技有限公司 | Method for promoting germination of loosestrife seeds |
| CN110786209A (en) * | 2019-11-28 | 2020-02-14 | 河南中医药大学 | Cultivation method for improving survival rate of selfheal seedlings and yield of selfheal |
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