CN106831188B

CN106831188B - High-quality tremella cultivation medium and preparation method thereof

Info

Publication number: CN106831188B
Application number: CN201710165808.9A
Authority: CN
Inventors: 张平; 黄勇云; 陈祥珍; 黄轩云; 陈高汕; 黄暖云
Original assignee: Fujian Cloud Biotechnology Development Co ltd
Current assignee: Fujian Cloud Biotechnology Development Co ltd
Priority date: 2017-03-20
Filing date: 2017-03-20
Publication date: 2020-06-23
Anticipated expiration: 2037-03-20
Also published as: CN106831188A

Abstract

A high-quality tremella cultivation medium and a preparation method thereof are disclosed, wherein the medium comprises 20-70 parts of olive branches, 20-60 parts of palm shreds, 10-40 parts of lotus seed shells, 9-20 parts of palm dregs, 5-10 parts of yeast protein and 1-2 parts of gypsum powder. According to the method, natural olive branches and palm shreds are used as main carbon source substrates for cultivating the tremella, cotton seed hulls can be completely replaced, the yield of the tremella can be kept to be the same as that of the tremella cultivated by the cotton seed hulls, the cost can be reduced, the taste of the tremella can be improved, and pesticide residues can be completely eradicated; in addition, the invention also adopts yeast protein and fermented palm meal as main nitrogen sources for cultivating the tremella, thereby not only having high quality and environmental protection, but also reducing the cost and enabling the tremella to be softer and more glutinous and more smooth in taste.

Description

High-quality tremella cultivation medium and preparation method thereof

Technical Field

The invention relates to a culture medium for tremella and a preparation method thereof, in particular to a culture medium for high-quality tremella and a preparation method thereof.

Background

Tremella is also called as Tremella, Tremella seed, etc., and is also called as "crown of fungus". The tremella is sweet and light in taste and neutral in nature, has the effects of tonifying spleen and promoting appetite, and also has the effects of benefiting qi, clearing intestines, nourishing yin and moistening lung; tremella can enhance immunity and improve tolerance of tumor patients to radiotherapy and chemotherapy. Tremella is rich in natural plant colloid, has effects of nourishing yin, and can be used as skin caring food for long-term administration.

The main raw material for cultivating the tremella is broadleaf weed wood, the limitation of forest cutting is caused, the higher cultivating yield of the cottonseed hulls is found later, and most of farmers including the ancient field in recent years use the cottonseed hulls as the cultivating substrate of the tremella. Cotton is not a food, and many pesticides are used in cultivation. According to food standards, the raw materials of the cotton seed hulls are basically overproof pesticide residues, and in addition, the cotton seed hulls contain gossypol, so that the tremella is not as good as the tremella cultivated by natural basswood or sawdust in taste. The control of raw materials is strict during the authentication of the organic edible fungi, and 100 percent of cottonseed hulls cannot be closed. For years, industry personnel always search raw materials for replacing cottonseed hulls, but the problems of high cost and low yield are not solved all the time.

The invention has the following patent: the edible fungi culture material prepared by utilizing the palm byproduct and the preparation method thereof (No. CN 103910583B) disclose that the palm husk is the shell of the palm fruit peeled off in the palm oil production process, the palm husk and the palm husk have a thicker layer of hard leather shell, and the palm husk and the palm shell are not easy to be crushed into materials with proper size, and are usually used as high-added-value active carbon; the palm pulp residue in the patent is the pulp remained after palm fruit oil extraction, the fiber content is low, the particles are fine, the air permeability is poor, in addition, the oil content is too high, and hypha germination can be inhibited if the oil content is too high. Different edible fungi have different lignin and cellulose decomposing capacities, and white fungus is a fungus with a weaker decomposing capacity, and a carbon source substrate with higher oil content is not adopted in the production of the edible fungi. Before oil extraction, palm kernels are ground into fine powder in order to improve the oil extraction rate, the particles of palm kernel meal in the patent are also fine, the fat content is high, multiple experiments of the applicant show that the bacterium walking speed is slowed down when the addition amount exceeds 10%, residues after oil extraction are used as substitute materials in edible fungus cultivation, the addition amount can not break through 15% all the time, and data show that the optimal addition amount of tea meal in edible fungus is 5%. Therefore, the palm kernel meal in the patent cannot be directly applied as a culture medium for tremella.

Disclosure of Invention

The invention provides a high-quality tremella cultivation medium and a preparation method thereof, and mainly aims to overcome the defects of high cost and low yield of tremella produced by the conventional tremella cultivation medium.

In order to solve the technical problems, the invention adopts the following technical scheme:

a high-quality tremella cultivation medium comprises the following components in parts by weight: 20-70 parts of olive branches, 20-60 parts of palm shreds, 10-40 parts of lotus seed shells, 9-20 parts of palm meal, 5-10 parts of yeast protein and 1-2 parts of gypsum powder.

Preferably, the weight portions are as follows: 55 parts of olive branches; 20 parts of palm filaments, 10 parts of lotus seed shells, 9 parts of palm meal, 5 parts of yeast protein and 1 part of gypsum powder.

Preferably, the weight portions are as follows: 55 parts of palm silk, 20 parts of olive branches, 10 parts of lotus seed shells, 9 parts of palm meal, 5 parts of yeast protein and 1 part of gypsum powder.

A preparation method of a culture medium for high-quality tremella comprises the following steps:

1) the fermentation of the palm meal, namely ① piling the palm meal into a stack with the height of 1.4-1.8 m, the width of 1.8-2.2 m and the length of more than 2m, wherein the view field can be flexible, 5-6 cm holes are drilled every 1m until two thirds of the stack, ② water is slowly sprayed until the bottom seeps out, watering is stopped, and the stack is naturally fermented, ③ pile turning is carried out when the temperature of the material core reaches 65 ℃ and the material core cannot continuously rise, ④ repeats the step ①, the fermentation is finished when the temperature of the material core reaches 65 ℃ again and the material core cannot continuously rise, and the temperature is reduced to the normal temperature by spreading;

2) taking 20-70 parts of waste olive branches, drying in the sun, and then crushing, wherein the crushed olive branches are not more than 0.8 cm; taking 20-60 parts of palm fibers in a natural form of short fibers; taking 10-40 parts of lotus seed shells, and crushing the lotus seed shells, wherein the diameter of the lotus seed shells is not more than 0.8 cm;

3) mixing 5-10 parts of yeast protein, 1-2 parts of gypsum powder and 9-20 parts of palm meal prepared in the step 1 with the components prepared in the step 2, adding water until the water content of the substrate reaches 62%, fully mixing, and enabling the materials to fully absorb water to obtain a tremella cultivation substrate;

4) filling the tremella cultivation medium prepared in the step 3 into a culture bag or a culture tank; then punching holes according to different modes of the bagged fungus sticks or the canned culture tank, and punching the holes on the fungus sticks or sealing the culture tank;

5) feeding the culture bag or the culture tank into a sterilization room, and firstly, controlling the temperature of the sterilization room to be 100-110 ℃ and the time to be 18-22 min; thirdly, adjusting the temperature of the sterilization room to 105-115 ℃, and controlling the time to be 27-32 min; thirdly, adjusting the temperature of the sterilization room to 120-130 ℃, and controlling the time to be 120-180 min; finally, turning off the heating device in the sterilization room, and sealing the culture bag or the culture tank in the sterilization room for 100-140 min;

6) feeding the culture bag or the culture tank into a refrigeration room, firstly pumping filtered outside air into the refrigeration room to reduce the temperature in the refrigeration room to 32-38 ℃, and secondly, starting refrigeration to control the temperature to 18-22 ℃ and the time to 10-14 hours;

7) inoculating according to a sterile operation procedure;

8) feeding the culture bag or the culture tank into a fungus culturing room, wherein in the early stage, the temperature in the fungus culturing room is controlled to be 26-28 ℃, the time is controlled to be 7-8 days, the humidity value is controlled to be 50-75% RH, and the concentration of carbon dioxide is controlled to be 2000-4500 ppm; in the middle stage, the temperature in the fungus culturing room is controlled to be 23-25 ℃, the time is controlled to be 8-12 days, the humidity value is controlled to be 50-75% RH, and the concentration of carbon dioxide is controlled to be 2000-4500 ppm; in the later stage, the temperature in the fungus culturing room is controlled to be 21-22 ℃, the time is controlled to be 12-18 days, the humidity value is controlled to be 50-75% RH, and the concentration of carbon dioxide is controlled to be 2000-4500 ppm;

9) and (4) conveying the culture bag or the culture tank into a mushroom growing room, and performing mushroom growing treatment in the mushroom growing room.

Further, in the step 1, when the palm meal is turned, the materials on the surface and at the bottom of the palm meal are turned to the upper layer of the center.

Further, the yeast protein is high-quality dried yeast powder with the protein content of more than 50%.

Further, the palm silk is short palm silk which is obtained by rolling together a mature palm flower shaft and a receptacle on the palm flower shaft, breaking the receptacle, and separating the palm silk from the receptacle.

The olive is evergreen tree of subtropical zone of Olea of Oleaceae, and the branch contains rich cellulose and hemicellulose, and has high lignin content, compact structure and good water retention property. It has been shown that the stems of olives are rich in flavonoids. Olive trees need to be pruned 2 times a year, and a large number of branches are abandoned.

The palm fiber squeezing method mainly adopts natural palm fibers of the oil palm, mainly exists in a mature flower support of the palm, the flower support of the oil palm is large, the size of the flower support is about (35-45) × (55-65) cm, when the palm oil is squeezed, a certain process is carried out, the whole fruit is peeled off from a flower axis and separated from the flower axis, the expanded flower support is still on the flower axis, the flower axis is also commonly called a stringing rod, the stringing rod and the flower support have no relation with the squeezed palm oil, the stringing rod is processed in a mode that the stringing rod comprises the flower support, the flower support is rolled together, then long palm fibers and short palm fibers are separated from the flower support, the long palm fibers can be used for manufacturing mattresses and other articles, and the short palm fibers are generally discarded.

Compared with the prior art, the invention has the beneficial effects that:

1. according to the method, natural olive branches and palm shreds are used as main carbon source substrates for cultivating the tremella, cotton seed hulls can be completely replaced, the yield of the tremella can be kept to be the same as that of the tremella cultivated by the cotton seed hulls, the cost can be reduced, the taste of the tremella can be improved, and pesticide residues can be completely eradicated; in addition, the invention also adopts yeast protein and fermented palm meal as main nitrogen sources for cultivating the tremella, thereby not only having high quality and environmental protection, but also reducing the cost and enabling the tremella to be softer and more glutinous and more smooth in taste.

2. The tremella produced by the method of the invention is high-quality and organic, the quality is greatly improved, and none of the tremella is detected by SGS-408 pesticide residue detection of the international detection organization.

3. According to the invention, the waste olive branches and palm shreds are utilized, the requirements of tremella on lignin and cellulose are met in the aspect of carbon sources by the formula of the culture medium, and the yield is ensured while the product quality is improved; the culture medium has no pesticide residue pollution, so that the cost is reduced, the resources are circulated, and the ecological environment is protected; is suitable for various tremella cultivation modes, is simple to operate and is easy to popularize.

4. The invention takes the short palm silk as one of the carbon source matrixes for cultivating the tremella. The advantages are as follows: the palm fiber is thinner than long palm fibers and only 1-3 cm in length, and is not subjected to any treatment such as crushing and the like, and is directly utilized after being dried in the sun; the nutrition is rich, and the carbon and nitrogen are reasonable; the content of lignin and cellulose is similar to that of cotton seed hulls; the palm fiber has a good structure and strong water retention; the material has certain elasticity, and can still keep air permeability after being tightly filled; the raw materials are sufficient in source and suitable for large-scale production.

5. According to the invention, the palm meal is subjected to fermentation treatment, the fat content is reduced, and macromolecular substances are decomposed, so that the bacterium-walking speed is higher after inoculation, the problem that the bacterium-walking speed is slowed down when the addition amount of the palm meal exceeds more than 10% in the prior art can be solved, and the productivity is improved.

Detailed Description

The following describes specific embodiments of the present invention.

Example one

A high-quality tremella cultivation medium comprises the following components in parts by weight: 55 parts of olive branches; 20 parts of palm filaments, 10 parts of lotus seed shells, 9 parts of palm meal, 5 parts of yeast protein and 1 part of gypsum powder.

Example two

A high-quality tremella cultivation medium comprises the following components in parts by weight: 55 parts of palm silk, 20 parts of olive branches, 10 parts of lotus seed shells, 9 parts of palm meal, 5 parts of yeast protein and 1 part of gypsum powder.

step one

The fermentation of the palm meal comprises the steps of piling the palm meal into pieces with the height of 1.4-1.8 m, the width of 1.8-2.2 m and the length of more than 2m by ①, maneuvering a view field, drilling a hole with the length of 5-6 cm every 1m till two thirds of the piled material to be convenient for ventilation, slowly spraying water by ② until the bottom seeps out, stopping watering, naturally fermenting, turning over when the temperature of a material core reaches 65 ℃ and cannot be continuously raised, repeating the step ① by ④, ending the fermentation when the temperature of the material core reaches 65 ℃ again and cannot be continuously raised, and spreading and cooling till the normal temperature.

Wherein, when the palm dregs are turned, the materials on the surface and the bottom of the palm dregs are turned to the upper layer of the center.

According to the invention, the palm meal is subjected to fermentation treatment, the fat content is reduced, and macromolecular substances are decomposed, so that the bacterium-walking speed is higher after inoculation, the problem that the bacterium-walking speed is slowed down when the addition amount of the palm meal exceeds more than 10% in the prior art can be solved, and the productivity is improved.

Step two

Taking 20-70 parts of waste olive branches, drying in the sun, and then crushing, wherein the crushed olive branches are not more than 0.8 cm; taking 20-60 parts of palm fibers in a natural form of short fibers; taking 10-40 parts of lotus seed shells, crushing the lotus seed shells, wherein the diameter of the lotus seed shells is not more than 0.8 cm.

The palm silk is short palm silk which is obtained by grinding mature palm flower axis and receptacle on the palm flower axis together, breaking the receptacle, and separating from the receptacle; the lotus seed shell is naturally dried in the sun.

According to the invention, the waste olive branches and palm shreds are utilized, the requirements of tremella on lignin and cellulose are met in the aspect of carbon sources by the formula of the culture medium, and the yield is ensured while the product quality is improved; the culture medium has no pesticide residue pollution, so that the cost is reduced, the resources are circulated, and the ecological environment is protected; is suitable for various tremella cultivation modes, is simple to operate and is easy to popularize.

In addition, the invention takes the short palm silk as one of the carbon source substrates for cultivating the tremella. The advantages are as follows: the palm fiber is thinner than long palm fibers and only 1-3 cm in length, and is not subjected to any treatment such as crushing and the like, and is directly utilized after being dried in the sun; the nutrition is rich, and the carbon and nitrogen are reasonable; the content of lignin and cellulose is similar to that of cotton seed hulls; the palm fiber has a good structure and strong water retention; the material has certain elasticity, and can still keep air permeability after being tightly filled; the raw materials are sufficient in source and suitable for large-scale production.

Step three

Mixing 5-10 parts of yeast protein, 1-2 parts of gypsum powder and 9-20 parts of palm meal prepared in the first step with the components prepared in the second step, adding water until the water content of the substrate reaches 62%, fully mixing, and enabling the materials to fully absorb water to form a high-quality tremella cultivation substrate;

wherein the yeast protein is high-quality dried yeast powder with protein content of more than 50%.

Step four

Filling the tremella cultivation medium prepared in the third step into a cultivation bag or a cultivation tank; then punching holes according to different modes of the bagged fungus sticks or the canned culture tanks, and punching the holes on the fungus sticks or sealing the culture tanks. The method mainly adopts a culture tank, and the operation flow of the culture tank is as follows:

①, loading the tremella cultivation medium into a culture tank through a full-automatic canning machine, automatically outputting the culture tank, loading the culture tank into a special culture tank, and automatically outputting the culture tank;

②, automatic punching, namely punching a plurality of holes on the substrate in the culture tank at one time and automatically outputting the culture tank;

③, automatic inner cover covering, namely, automatically covering the inner cover on the mouth of the culture tank by an inner cover covering machine and automatically outputting the culture tank;

④, automatically covering the outer cover, namely automatically covering the outer cover on the mouth of the culture tank by an outer cover machine and automatically outputting the culture tank;

⑤, automatic stacking, namely, automatically stacking the culture tanks by a stacker crane and then sending the culture tanks to a subsequent process.

Step five

Feeding the culture bag or the culture tank into a sterilization room, and firstly, controlling the temperature of the sterilization room to be 100-110 ℃ and the time to be 18-22 min; thirdly, adjusting the temperature of the sterilization room to 105-115 ℃, and controlling the time to be 27-32 min; thirdly, adjusting the temperature of the sterilization room to 120-130 ℃, and controlling the time to be 120-180 min; and finally, turning off the heating device in the sterilization room, and sealing the culture bag or the culture tank in the sterilization room for 100-140 min.

Step six

And (3) feeding the culture bag or the culture tank into a refrigeration room, firstly pumping the filtered outside air into the refrigeration room to reduce the temperature in the refrigeration room to 32-38 ℃, and secondly, starting refrigeration to control the temperature to 18-22 ℃ and the time to 10-14 hours.

Step seven

Inoculation was performed according to sterile protocol. This sterile protocol is conventional in the art and therefore is not described in detail herein.

Step eight

Feeding the culture bag or the culture tank into a fungus culturing room, wherein in the early stage, the temperature in the fungus culturing room is controlled to be 26-28 ℃, the time is controlled to be 7-8 days, the humidity value is controlled to be 50-75% RH, and the concentration of carbon dioxide is controlled to be 2000-4500 ppm; in the middle stage, the temperature in the fungus culturing room is controlled to be 23-25 ℃, the time is controlled to be 8-12 days, the humidity value is controlled to be 50-75% RH, and the concentration of carbon dioxide is controlled to be 2000-4500 ppm; and in the later stage, the temperature in the fungus culturing room is controlled to be 21-22 ℃, the time is controlled to be 12-18 days, the humidity value is controlled to be 50-75% RH, and the concentration of carbon dioxide is controlled to be 2000-4500 ppm.

Step nine

And (4) conveying the culture bag or the culture tank into a mushroom growing room, and performing mushroom growing treatment in the mushroom growing room. The culture bags are managed according to a conventional management mode for tremella growth, and the culture tanks are managed according to a tremella factory production process. Since it is a prior art, it is not described herein again.

The tremella produced by the method of the invention is high-quality and organic, the quality is greatly improved, and none of the tremella is detected by SGS-408 pesticide residue detection of the international detection institution; in addition, the invention utilizes the waste olive branches and palm shreds, thereby not only reducing the cost, but also recycling resources, being ecological and environment-friendly, and being suitable for various tremella cultivation modes.

Example one

step one

The fermentation of the palm meal comprises the steps of ① piling the palm meal into pieces with the height of 1.6m, the width of 2m and the length of more than 2m, wherein the view field can be flexible, 5cm holes are drilled every 1m until two thirds of the piled material is convenient to ventilate, ② slowly drenching water until the bottom seeps out, stopping watering and naturally fermenting, ③ turning the piled material when the temperature of the material core reaches 65 ℃ and cannot be continuously raised, ④ repeating the step ①, ending the fermentation when the temperature of the material core reaches 65 ℃ again and cannot be continuously raised, and spreading and cooling until the temperature is normal.

Step two

Taking 55 parts of waste olive branches, drying in the sun, and then crushing, wherein the crushed olive branches are not more than 0.8 cm; taking 20 parts of palm filaments in a short filament natural form; taking 10 parts of lotus seed shells, crushing the lotus seed shells, and ensuring that the diameter of the lotus seed shells is not more than 0.8 cm.

Step three

And (3) mixing 5 parts of yeast protein, 1 part of gypsum powder and 9 parts of palm meal prepared in the first step with the components prepared in the second step, adding water until the water content of the substrate reaches 62%, and fully mixing the components until the moisture is fully absorbed by the materials to form the high-quality tremella cultivation substrate.

Step four

Filling the tremella cultivation medium prepared in the third step into a culture tank; then punching according to a canning culture tank mode, and then sealing the culture tank.

Step five

Feeding the culture tank into a sterilization room, and firstly, controlling the temperature of the sterilization room at 105 ℃ and the time at 20 min; thirdly, adjusting the temperature of the sterilization room to 110 ℃ and controlling the time to be 30 min; thirdly, adjusting the temperature of the sterilization room to 125 ℃ and controlling the time to be 150 min; finally, the heating device in the sterilization room is turned off, and the culture tank is sealed in the sterilization room for 120 min.

Step six

And (3) conveying the culture tank into a refrigerating room, firstly, pumping the filtered outside air into the refrigerating room to reduce the temperature in the refrigerating room to 35 ℃, and secondly, starting to refrigerate to control the temperature to be 20 ℃ and the time to be 12 hours.

Step seven

Inoculation was performed according to sterile protocol.

Step eight

The culture tank is sent into a fungus culturing room, the temperature in the fungus culturing room is controlled at 27 ℃ in the early stage, the time is controlled at 7 days, the humidity value is controlled at 60% RH, and the carbon dioxide concentration is controlled at 3000 ppm; in the middle stage, the temperature in the fungus culturing room is controlled at 24 ℃, the time is controlled at 10 days, the humidity value is controlled at 60% RH, and the carbon dioxide concentration is controlled at 3000 ppm; in the later period, the temperature in the fungus culturing room is controlled at 21 ℃, the time is controlled at 15 days, the humidity value is controlled at 60% RH, and the carbon dioxide concentration is controlled at 3000 ppm.

Step nine

And (4) conveying the culture tank into a fruiting room, and carrying out fruiting treatment in the fruiting room.

Example two

Step one

The fermentation of the palm meal comprises the steps of ① piling the palm meal into pieces with the height of 1.8m, the width of 2.2m and the length of more than 2m, wherein the view field can be flexible, a hole of 6cm is drilled every 1m until two thirds of the piled material is convenient to ventilate, ② slowly drenches water until the water is just seeped out from the bottom, the watering is stopped, the natural fermentation is carried out, ③, turning the piled material when the temperature of the material core reaches 65 ℃ and the material core cannot continuously rise, ④ repeats step ①, the fermentation is finished when the temperature of the material core reaches 65 ℃ again and the material core cannot continuously rise, and the fermentation is spread and cooled until the temperature is normal.

Step two

Taking 20 parts of waste olive branches, drying in the sun, and then crushing, wherein the crushed olive branches are not more than 0.8 cm; taking 55 parts of palm fibers in a natural shape of short fibers; taking 10 parts of lotus seed shells, crushing the lotus seed shells, and ensuring that the diameter of the lotus seed shells is not more than 0.8 cm.

Step three

Step four

Filling the tremella cultivation medium prepared in the third step into a cultivation tank; then punching holes according to the mode of the canned culture tank, and then sealing the culture tank.

Step five

Feeding the culture tank into a sterilization room, and firstly, controlling the temperature of the sterilization room at 100 ℃ and the time at 22 min; thirdly, adjusting the temperature of the sterilization room to 115 ℃ and controlling the time to be 28 min; thirdly, adjusting the temperature of the sterilization room to 120 ℃ and controlling the time to be 160 min; finally, the heating device in the sterilization room is turned off, and the culture bag or the culture tank is sealed in the sterilization room for 110 min.

Step six

And (3) feeding the culture tank into a refrigerating room, pumping the filtered outside air into the refrigerating room to reduce the temperature in the refrigerating room to 32 ℃, and starting to refrigerate to control the temperature to 22 ℃ and the time to 14 hours.

Step seven

Inoculation was performed according to sterile protocol.

Step eight

The culture tank is sent into a fungus culturing room, the temperature in the fungus culturing room is controlled at 26 ℃ in the early stage, the time is controlled at 8 days, the humidity value is controlled at 70% RH, and the carbon dioxide concentration is controlled at 4000 ppm; in the middle stage, the temperature in the fungus culturing room is controlled at 25 ℃, the time is controlled at 12 days, the humidity value is controlled at 70% RH, and the carbon dioxide concentration is controlled at 4000 ppm; in the later period, the temperature in the fungus culturing room is controlled at 22 ℃, the time is controlled at 16 days, the humidity value is controlled at 70% RH, and the carbon dioxide concentration is controlled at 4000 ppm.

Step nine

The results of comparing the growth of tremella fuciformis cultivated in the two examples in the preparation method in the cultivation material (taking the tremella flavicans as a sample) are shown in table 1. The recipe for the control blank CK was: 73 parts of cottonseed hulls, 25 parts of bran and 1 part of gypsum powder. Table 2, table 3, table 4 are the same.

Table 1 comparison of growth of tremella fuciformis cultivated in two examples of the manufacturing method in the cultivation material

	Cover time/d	Time of full tank/d	Hypha growth rate/cm/d	Hypha growth vigor anchor
					CK	10	17	0.55	+ + +
Example one	10	18	0.51	+ + +
					Example two	10	17	0.57	+ + +

Note: the growth of tremella hyphae, i.e., the degree of whiteness and the abundance of the tremella hyphae, is indicated by a plus sign. Preferably, "+ + + +" decreases and the growth progressively decreases.

As can be seen from Table 1, the hypha growth rate of the second example was the best, although the hypha growth rate of the formulations was almost the same.

The main nutrients of Tremella fuciformis (yellow ear samples) cultivated in different substrates are shown in Table 2.

TABLE 2 comparison of protein, fat and polysaccharide contents (g/100g) of Tremella fuciformis cultivated in two examples of preparation methods

The analysis in table 2 shows that the difference between the nutrients of the three formulas and the control group is not large, and the palm meal is added in the first and second examples, so that the fat content of the produced tremella is increased. Thus, the matrix of the invention can completely replace cotton seed hulls.

TABLE 3 Tremella cultivated with different media and yield

The analysis in table 3 shows that the difference of the formulas in terms of yield is small, but the prices of olive branches, palm shreds and lotus seed shells are cheaper than that of cotton seed shells, so that the tremella prepared by the preparation method is lower in production cost compared with the prior art.

The sensory impact of different substrate cultivated tremella (yellow ear as sample) is shown in table 4.

Tremella cultivated with different substrates (using Tremella flavicans as sample) has influence on sensory evaluation standard

Note: the stability of the soup refers to whether the white fungus is separated from the soup after the white fungus soup is stirred and placed for more than half an hour.

TABLE 4 influence of Tremella fuciformis (sampled from Tremella flava) cultivated with different media on sensory evaluation

The analysis of table 4 shows that the differences of various formula groups are small through sensory evaluation and comparison, the main difference exists in the fragrance and the taste, the fragrance is not as good as that of the tremella obtained in the first embodiment and the second embodiment due to the fact that the cottonseed hulls contain gossypol, the taste is not fresh and sweet enough, and the palm meal has a large influence on the taste of the tremella.

The advantages of the invention are: the formula of the culture medium meets the requirements of tremella on lignin and cellulose in terms of carbon sources, so that the yield is ensured, and the formula is not complex and is easy to popularize to mushroom farmers; the tremella products produced by the tremella cultivation medium have good quality, fragrant taste and good mouthfeel, and none of the tremella products is detected by SGS-408 pesticide residues detected by an international detection organization; the production cost of the tremella is reduced, and consumers can taste high-quality tremella which is cheap and good.

Meanwhile, the culture medium is also suitable for culturing edible fungi such as hericium erinaceus and agrocybe cylindracea.

The above description is only an embodiment of the present invention, but the design concept of the present invention is not limited thereto, and any insubstantial modifications made by using the design concept should fall within the scope of infringing the present invention.

Claims

1. A high-quality tremella cultivation medium is characterized by comprising the following components in parts by weight: 20-70 parts of olive branches, 20-60 parts of palm shreds, 10-40 parts of lotus seed shells, 9-20 parts of palm meal, 5-10 parts of yeast protein and 1-2 parts of gypsum powder;

2) taking 20-70 parts of waste olive branches, drying in the sun, and then crushing, wherein the crushed olive branches are not more than 0.8 cm; taking 20-60 parts of palm fibers in a natural form of short fibers; taking 10-40 parts of lotus seed shells, and crushing the lotus seed shells, wherein the diameter of the lotus seed shells is not more than 0.8 cm; the palm fibers are natural palm fibers of oil palm trees; the palm silk is prepared by grinding mature palm flower axis including flower receptacle thereon together, grinding the flower receptacle, and separating short palm silk from the flower receptacle;

7) inoculating according to a sterile operation procedure;

2. The culture medium for high-quality tremella as claimed in claim 1, wherein: the weight portions are as follows: 55 parts of olive branches; 20 parts of palm filaments, 10 parts of lotus seed shells, 9 parts of palm meal, 5 parts of yeast protein and 1 part of gypsum powder.

3. The culture medium for high-quality tremella as claimed in claim 1, wherein: the weight portions are as follows: 55 parts of palm silk, 20 parts of olive branches, 10 parts of lotus seed shells, 9 parts of palm meal, 5 parts of yeast protein and 1 part of gypsum powder.

4. The method for preparing the culture medium for high-quality tremella as claimed in claim 1, wherein: in the step 1, materials on the surface and the bottom of the palm meal are turned to the upper layer of the center when the palm meal is turned.

5. The method for preparing the culture medium for high-quality tremella as claimed in claim 1, wherein: the yeast protein is high-quality dry yeast powder with the protein content of more than 50 percent.