CN106822650A - Tiny ecosystem Chinese medicine preparation and preparation method thereof - Google Patents
Tiny ecosystem Chinese medicine preparation and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/254—Acanthopanax or Eleutherococcus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/894—Dioscoreaceae (Yam family)
- A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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Abstract
The invention discloses a kind of Tiny ecosystem Chinese medicine preparation and preparation method thereof; using probiotics direct fermentation Chinese medicine; active ingredient of Chinese herbs is protected to exempt from destruction; produce new drug effect; and various probiotic mixed fermentation Chinese medicines; Effective Component of Chinese Medicine can be enable to discharge to greatest extent, while cellulose can be changed into oligosaccharide, promote the growth of beneficial bacterium in intestines and stomach.
Description
Technical field
The invention belongs to microbial fermentation technical field of traditional Chinese medicines, it is related to a kind of Tiny ecosystem Chinese medicine preparation and preparation method thereof.
Background technology
Tiny ecosystem Chinese medicine preparation is managed according to subjects such as traditional Chinese medicine and pharmacy, medical science microecology, microbiology, nutrition
By, in the preparation of traditional help class Chinese medicine preparation, the fermentation of the beneficial microbes such as probiotic bacteria is introduced, and using enzyme hydrolysis strain
Oral class Chinese medicine preparation prepared by the modern biotechnologies such as culture medium.Tiny ecosystem Chinese medicine preparation collection traditional Chinese medicine compound preparation and Tiny ecosystem
, its peculiar clinical effectiveness is far beyond conventional formulation for the dual-use function of preparation.
For example same fermentation tank of the enteron aisle of human body, absorption and utilization to taking in herbal function composition, often be unable to do without intestines
The participation of road normal flora.Chemicals is usually small-molecule substance, and often composition is single, purity is high, the polarity of medicine, point
Sub-structural features etc. are all relatively clear and definite, it is oral after can directly pass through small intestinal mucosa barrier cell, into blood performance pharmacological action.
Different from chemicals, compound Chinese medicinal preparation is a mixture system for complexity, the existing low molecular compound of its functional component,
Also there are Large molecular conjugates.Traditional Chinese medicine compound preparation will give play to clinical effectiveness, and clinical effect can be embodied depending on whether the medicine contains
The functional component of fruit, and patient can effectively absorb, using two aspects of these functional components, and the latter is to a great extent
Depending on the microecological balance situation of Intestinal Mucosal Injury in Patients Undergoing.But has a problem in that Healthy People is not taken medicine, and sick people, the people of prolonged illness is often
There is intestinal microecology unbalance.Especially get old and weak many patients, major operation after reconvalescent or chemicotherapy tumor patient,
The Tiny ecosystem of its body enteron aisle often serious unbalance, this constrains biological utilisation of the body to taken in herbal function composition again
Degree.This is the contradiction that should not be overlooked, but is muchly ignored by us.It is influence tradition that intestinal microecology is unbalance
Chinese medicine preparation gives full play to a key factor of curative effect, therefore research for Tiny ecosystem Chinese medicine preparation seems increasingly important.
The content of the invention
It is an object of the invention to overcome the shortcomings of above-mentioned technology, there is provided a kind of Tiny ecosystem Chinese medicine preparation composition；It
Including：Bacillus subtilis, bacillus licheniformis, animal bifidobacteria, lactic acid bacteria, saccharomyces cerevisiae ratio are：20~30：
10~15：45~50：10~30：10~20.In zymotic fluid used by Tiny ecosystem Chinese medicine composition add Chinese medicine into
Point, affiliated traditional Chinese medicine ingredients include the Radix Astragali, the bighead atractylodes rhizome, Radix Codonopsis, matrimony vine, folium isatidis, honeysuckle, cordate houttuynia, radix bupleuri, selfheal, osmanthus
Skin, Radix Glycyrrhizae.
The technical scheme that the object of the invention is used is as follows：Described a kind of Tiny ecosystem Chinese medicine preparation, it is characterised in that will
Chinese medicine extract and the mixing of corn and soybean extract solution are obtained by the method for probiotics fermention.A kind of described Tiny ecosystem Chinese medicine
Formulation preparation method, comprises the following steps：
1）The preparation of Chinese medicine extract
The herb powder after crushing is weighed in proportion, by solid-liquid ratio 1:After 10 soak 30min, it is heated to 100 DEG C and boils 1.5
Hour, filtrate is collected in filtering；The dregs of a decoction press solid-liquid ratio 1:6 soak, and are heated to 100 DEG C and boil 1 hour, filtering, collect filter
Liquid, twice filtrate merging is concentrated under reduced pressure, standby；
2）The preparation of corn and soybean enzymolysis liquid
In mass ratio 1:1 weighs the corn and soybean powder mixing and stirring after crushing；By solid-liquid ratio 1:3 soak
60min, adjusts solution pH value to 6.5-7.0 after immersion；Protease is added, 60 DEG C of water-bath keeps constant temperature 5 hours；Solution is adjusted again
PH value adds 65 DEG C of carbohydrase water-bath to keep constant temperature 4 hours to 4.5-5.0；Filtering, collects filtrate, is concentrated under reduced pressure standby；
3）Zymotechnique
To be inoculated in optimum medium respectively in lactic acid bacteria, saccharomycete, primary seed solution is cultivated under suitable condition, when each
Bacteria growing is enlarged culture to being transferred to respectively during exponential phase in secondary seed fermentation tank, stop when reaching exponential phase
Only cultivate, it is standby；
By above-mentioned Chinese medicine extract solution and soybean extract by volume 1:1 mixing, 100 DEG C of sterilizing 20min, after cooling, respectively
Addition accounts for the lactic acid bacterial liquid and 5% saccharomycete bacterium solution of extract solution quality 5%, and ferment 8-12h at 30 DEG C, is stirred every 8h
Mix once；When total viable count reaches 1.0 × 108 - 9Stop fermentation during CFU/ml, packing is made Tiny ecosystem Chinese medicine preparation.
Described a kind of Tiny ecosystem Chinese medicine preparation, it is characterised in that described Chinese medicine presses mass fraction includes ginseng 1-10
Part, Chinese yam 1-10 parts, matrimony vine 1-10 parts, Cordyceps militaris 1-10 parts, Radix Glycyrrhizae 1-10 parts, Sessileflower Acanthopanax Bark 1-10 parts.
Described a kind of Tiny ecosystem Chinese medicine preparation, it is characterised in that described probiotics is included in lactic acid bacteria or saccharomycete
Any or two kinds.
The invention provides a kind of Tiny ecosystem Chinese medicine preparation and preparation method thereof, using probiotics direct fermentation Chinese medicine, protect
Active ingredient of Chinese herbs is protected and has exempted from destruction, produced new drug effect, and various probiotic mixed fermentation Chinese medicines, Effective Component of Chinese Medicine can have been made
Discharged to greatest extent, while cellulose can be changed into oligosaccharide, promoted the growth of beneficial bacterium in intestines and stomach.
Specific embodiment
Embodiment 1
1）The preparation of Chinese medicine extract
Chinese medicine：10 parts of ginseng, 6 parts of Chinese yam, 3 parts of matrimony vine, 1 part of Cordyceps militaris, 3 parts of Radix Glycyrrhizae, 5 parts of Sessileflower Acanthopanax Bark.
The herb powder after crushing is weighed by above-mentioned formula, by solid-liquid ratio 1:After 10 soak 30min, 100 are heated to
DEG C boil 1.5 hours, filter, collect filtrate；The dregs of a decoction press solid-liquid ratio 1:6 soak, and are heated to 100 DEG C and boil 1 hour, mistake
Filter, collects filtrate, and filtrate merges concentrated under reduced pressure twice, standby.
2）The preparation of corn and soybean enzymolysis liquid
In mass ratio 1:1 weighs the corn and soybean powder mixing and stirring after crushing；By solid-liquid ratio 1:3 soak
60min, adjusts solution pH value to 6.5-7.0 after immersion；Protease is added, 60 DEG C of water-bath keeps constant temperature 5 hours；Solution is adjusted again
PH value adds 65 DEG C of carbohydrase water-bath to keep constant temperature 4 hours to 4.5-5.0；Filtering, collects filtrate, is concentrated under reduced pressure standby.
3）Zymotechnique
Lactic acid bacteria, saccharomycete are inoculated into culture medium respectively, at 37 DEG C, 200rpm shaking table cultures produce first order seed
Liquid, when bacterial content reaches 107-8During CFU/ml or so, primary seed solution is inoculated into secondary seed with inoculum concentration 5% and is sent out
Amplification Culture in fermentation tank, cultivation temperature is 28~35 DEG C, and rotating speed is 200rpm, when gemma bacterial content reaches 109CFU/ml
During left and right, stop fermentation, it is standby；
By above-mentioned Chinese medicine extract solution and soybean extract by volume 1:1 mixing, 100 DEG C of sterilizing 20min, after cooling, respectively
Addition accounts for the lactic acid bacterial liquid and 5% saccharomycete bacterium solution of extract solution quality 5%, and ferment 8-12h at 30 DEG C, is stirred every 8h
Mix once；When total viable count reaches 1.0 × 108 - 9Stop fermentation during CFU/ml, packing is made Tiny ecosystem Chinese medicine preparation.
Embodiment 2
1）The preparation of Chinese medicine extract
Chinese medicine：5 parts of ginseng, 2 parts of Chinese yam, 3 parts of matrimony vine, 2 parts of Cordyceps militaris, 2 parts of Radix Glycyrrhizae, 6 parts of Sessileflower Acanthopanax Bark.
The herb powder after crushing is weighed by above-mentioned formula, by solid-liquid ratio 1:After 10 soak 30min, 100 are heated to
DEG C boil 1.5 hours, filter, collect filtrate；The dregs of a decoction press solid-liquid ratio 1:6 soak, and are heated to 100 DEG C and boil 1 hour, mistake
Filter, collects filtrate, and filtrate merges concentrated under reduced pressure twice, standby.
2）The preparation of corn and soybean enzymolysis liquid
In mass ratio 1:1 weighs the corn and soybean powder mixing and stirring after crushing；By solid-liquid ratio 1:3 soak
60min, adjusts solution pH value to 6.5-7.0 after immersion；Protease is added, 60 DEG C of water-bath keeps constant temperature 5 hours；Solution is adjusted again
PH value adds 65 DEG C of carbohydrase water-bath to keep constant temperature 4 hours to 4.5-5.0；Filtering, collects filtrate, is concentrated under reduced pressure standby.
3）Zymotechnique
Lactic acid bacteria, saccharomycete are inoculated into culture medium respectively, at 37 DEG C, 200rpm shaking table cultures produce first order seed
Liquid, when bacterial content reaches 107-8During CFU/ml or so, primary seed solution is inoculated into secondary seed with inoculum concentration 5% and is sent out
Amplification Culture in fermentation tank, cultivation temperature is 28~35 DEG C, and rotating speed is 200rpm, when gemma bacterial content reaches 109CFU/ml
During left and right, stop fermentation, it is standby；
By above-mentioned Chinese medicine extract solution and soybean extract by volume 1:1 mixing, 100 DEG C of sterilizing 20min, after cooling, respectively
Addition accounts for the lactic acid bacterial liquid and 5% saccharomycete bacterium solution of extract solution quality 5%, and ferment 8-12h at 30 DEG C, is stirred every 8h
Mix once；When total viable count reaches 1.0 × 108 - 9Stop fermentation during CFU/ml, packing is made Tiny ecosystem Chinese medicine preparation.
Embodiment 3
1）The preparation of Chinese medicine extract
Chinese medicine：2 parts of ginseng, 7 parts of Chinese yam, 1 part of matrimony vine, 1 part of Cordyceps militaris, 4 parts of Radix Glycyrrhizae, 4 parts of Sessileflower Acanthopanax Bark.
The herb powder after crushing is weighed by above-mentioned formula, by solid-liquid ratio 1:After 10 soak 30min, 100 are heated to
DEG C boil 1.5 hours, filter, collect filtrate；The dregs of a decoction press solid-liquid ratio 1:6 soak, and are heated to 100 DEG C and boil 1 hour, mistake
Filter, collects filtrate, and filtrate merges concentrated under reduced pressure twice, standby.
2）The preparation of corn and soybean enzymolysis liquid
In mass ratio 1:1 weighs the corn and soybean powder mixing and stirring after crushing；By solid-liquid ratio 1:3 soak
60min, adjusts solution pH value to 6.5-7.0 after immersion；Protease is added, 60 DEG C of water-bath keeps constant temperature 5 hours；Solution is adjusted again
PH value adds 65 DEG C of carbohydrase water-bath to keep constant temperature 4 hours to 4.5-5.0；Filtering, collects filtrate, is concentrated under reduced pressure standby.
3）Zymotechnique
Lactic acid bacteria, saccharomycete are inoculated into culture medium respectively, at 37 DEG C, 200rpm shaking table cultures produce first order seed
Liquid, when bacterial content reaches 107-8During CFU/ml or so, primary seed solution is inoculated into secondary seed with inoculum concentration 5% and is sent out
Amplification Culture in fermentation tank, cultivation temperature is 28~35 DEG C, and rotating speed is 200rpm, when gemma bacterial content reaches 109CFU/ml
During left and right, stop fermentation, it is standby；
By above-mentioned Chinese medicine extract solution and soybean extract by volume 1:1 mixing, 100 DEG C of sterilizing 20min, after cooling, respectively
Addition accounts for the lactic acid bacterial liquid of extract solution quality 5%, and ferment 8-12h at 30 DEG C, every 8h stirrings once；When viable bacteria is total
Number reaches 1.0 × 108 - 9Stop fermentation during CFU/ml, packing is made Tiny ecosystem Chinese medicine preparation.
Embodiment 4
1）The preparation of Chinese medicine extract
Chinese medicine：3 parts of ginseng, 5 parts of Chinese yam, 1 part of matrimony vine, 3 parts of Cordyceps militaris, 1 part of Radix Glycyrrhizae, 3 parts of Sessileflower Acanthopanax Bark.
The herb powder after crushing is weighed by above-mentioned formula, by solid-liquid ratio 1:After 10 soak 30min, 100 are heated to
DEG C boil 1.5 hours, filter, collect filtrate；The dregs of a decoction press solid-liquid ratio 1:6 soak, and are heated to 100 DEG C and boil 1 hour, mistake
Filter, collects filtrate, and filtrate merges concentrated under reduced pressure twice, standby.
2）The preparation of corn and soybean enzymolysis liquid
In mass ratio 1:1 weighs the corn and soybean powder mixing and stirring after crushing；By solid-liquid ratio 1:3 soak
60min, adjusts solution pH value to 6.5-7.0 after immersion；Protease is added, 60 DEG C of water-bath keeps constant temperature 5 hours；Solution is adjusted again
PH value adds 65 DEG C of carbohydrase water-bath to keep constant temperature 4 hours to 4.5-5.0；Filtering, collects filtrate, it is proposed that concentration is standby.
3）Zymotechnique
Lactic acid bacteria, saccharomycete are inoculated into culture medium respectively, at 37 DEG C, 200rpm shaking table cultures produce first order seed
Liquid, when bacterial content reaches 107-8During CFU/ml or so, primary seed solution is inoculated into secondary seed with inoculum concentration 5% and is sent out
Amplification Culture in fermentation tank, cultivation temperature is 28~35 DEG C, and rotating speed is 200rpm, when gemma bacterial content reaches 109CFU/ml
During left and right, stop fermentation, it is standby；
By above-mentioned Chinese medicine extract solution and soybean extract by volume 1:1 mixing, 100 DEG C of sterilizing 20min, after cooling, respectively
Addition accounts for the lactic acid bacterial liquid and 5% saccharomycete bacterium solution of extract solution quality 5%, and ferment 8-12h at 30 DEG C, is stirred every 8h
Mix once；When total viable count reaches 1.0 × 108 - 9Stop fermentation during CFU/ml, packing is made Tiny ecosystem Chinese medicine preparation.
Claims (4)
1. a kind of Tiny ecosystem Chinese medicine preparation, it is characterised in that：Chinese medicine extract and corn and soybean extract solution are mixed, by benefit
The method of raw bacterium fermentation is obtained.
2. a kind of Tiny ecosystem Chinese medicine preparation according to claim 1, it is characterised in that:Described Chinese medicine presses mass fraction
Including ginseng 1-10 parts, Chinese yam 1-10 parts, matrimony vine 1-10 parts, Cordyceps militaris 1-10 parts, Radix Glycyrrhizae 1-10 parts, Sessileflower Acanthopanax Bark
1-10 parts.
3. a kind of Tiny ecosystem Chinese medicine preparation preparation method, comprises the following steps：
1）The preparation of Chinese medicine extract
The herb powder after crushing is weighed in proportion, by solid-liquid ratio 1:After 10 soak 30min, it is heated to 100 DEG C and boils 1.5
Hour, filtrate is collected in filtering；The dregs of a decoction press solid-liquid ratio 1:6 soak, and are heated to 100 DEG C and boil 1 hour, filtering, collect filter
Liquid, twice filtrate merging is concentrated under reduced pressure, standby；
The preparation of corn and soybean enzymolysis liquid
In mass ratio 1:1 weighs the corn and soybean powder mixing and stirring after crushing；By solid-liquid ratio 1:3 soak
60min, adjusts solution pH value to 6.5-7.0 after immersion；Protease is added, 60 DEG C of water-bath keeps constant temperature 5 hours；Solution is adjusted again
PH value adds 65 DEG C of carbohydrase water-bath to keep constant temperature 4 hours to 4.5-5.0；Filtering, collects filtrate, is concentrated under reduced pressure, standby；
3）Zymotechnique
To be inoculated in optimum medium respectively in lactic acid bacteria, saccharomycete, primary seed solution is cultivated under suitable condition, when each
Bacteria growing is enlarged culture to being transferred to respectively during exponential phase in secondary seed fermentation tank, stop when reaching exponential phase
Only cultivate, it is standby；
By above-mentioned Chinese medicine extract solution and soybean extract by volume 1:1 mixing, 100 DEG C of sterilizing 20min, after cooling, respectively
Addition accounts for the lactic acid bacterial liquid and 5% saccharomycete bacterium solution of extract solution quality 5%, and ferment 8-12h at 30 DEG C, is stirred every 8h
Mix once；When total viable count reaches 1.0 × 108 - 9Stop fermentation during CFU/ml, packing is made Tiny ecosystem Chinese medicine preparation.
4. a kind of Tiny ecosystem Chinese medicine preparation preparation method according to claim 3, it is characterised in that described prebiotic bacterium bag
Include lactic acid bacteria and or saccharomycete.
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| CN201710173411.4A CN106822650A (en) | 2017-03-22 | 2017-03-22 | Tiny ecosystem Chinese medicine preparation and preparation method thereof |
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ID=59130161
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107373255A (en) * | 2017-08-09 | 2017-11-24 | 山东博华高效生态农业科技有限公司 | A kind of ferment drink and its preparation technology with spleen strengthening and stomach nourishing health effect |
| CN109329936A (en) * | 2018-09-12 | 2019-02-15 | 广东笑咪咪食品有限公司 | A kind of natural materials fermentation methods of extraction and preparation for candy production |
| CN109876019A (en) * | 2019-04-10 | 2019-06-14 | 新圣宝健康科技(长春)有限公司 | A kind of preparation method of Chinese medicine |
| CN111808901A (en) * | 2020-07-07 | 2020-10-23 | 华熙生物科技股份有限公司 | Preparation method of cordyceps militaris fermentation extract, product obtained by preparation method and application of product |
-
2017
- 2017-03-22 CN CN201710173411.4A patent/CN106822650A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107373255A (en) * | 2017-08-09 | 2017-11-24 | 山东博华高效生态农业科技有限公司 | A kind of ferment drink and its preparation technology with spleen strengthening and stomach nourishing health effect |
| CN109329936A (en) * | 2018-09-12 | 2019-02-15 | 广东笑咪咪食品有限公司 | A kind of natural materials fermentation methods of extraction and preparation for candy production |
| CN109876019A (en) * | 2019-04-10 | 2019-06-14 | 新圣宝健康科技(长春)有限公司 | A kind of preparation method of Chinese medicine |
| CN111808901A (en) * | 2020-07-07 | 2020-10-23 | 华熙生物科技股份有限公司 | Preparation method of cordyceps militaris fermentation extract, product obtained by preparation method and application of product |
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Application publication date: 20170613 |