CN106797728A - The efficient germinating method of balsam pear kind - Google Patents

The efficient germinating method of balsam pear kind Download PDF

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Publication number
CN106797728A
CN106797728A CN201710045960.3A CN201710045960A CN106797728A CN 106797728 A CN106797728 A CN 106797728A CN 201710045960 A CN201710045960 A CN 201710045960A CN 106797728 A CN106797728 A CN 106797728A
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Prior art keywords
seed
vernalization
hours
constant temperature
moisturizing
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CN201710045960.3A
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Chinese (zh)
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唐思兵
雷方全
其他发明人请求不公开姓名
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Leshan Agriculture Doctor Modern Agriculture Development Co Ltd
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Leshan Agriculture Doctor Modern Agriculture Development Co Ltd
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Priority to CN201710045960.3A priority Critical patent/CN106797728A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting

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  • Life Sciences & Earth Sciences (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses a kind of efficient germinating method of balsam pear kind, while seed is carried out into primary vernalization, hot and humid vernalization, low temperature and high relative humidity vernalization, medicine vernalization, the vernalization of moisturizing constant temperature and artificial germination, bud ratio is up to 100%;Seed can all sprout for 40 60 hours in insulating box, and germinating time is short, sprouts in good order, it is adaptable to which balsam pear kind is germinateed.

Description

The efficient germinating method of balsam pear kind
A kind of efficient germinating method of the technical field the present invention relates to germinating method, especially balsam pear kind.
Background technology balsam pear is the Curcurbitaceae herbal cultigen of annual climber, alias balsam pear, leprosy grape, category The warm heat tolerant vegetable of happiness.Seed of bitter gourd skin depth and hard, bad hydraulic permeability, germinate relatively difficult, and germination period is long, and bud ratio is also low, therefore Need to carry out artificial germination.Not only germinating time is long for the artificial germination method for currently using, and bud ratio is low, sprouts not whole Together.
" a kind of seed of bitter gourd quick pregermination processing method " patent (application disclosed in Chinese patent CN105165175A Number:201510538784.8), comprise the following steps:1. selecting improved seeds carries out high temperature drying treatment, can eliminate thin on seed Bacterium and germ;2. seed is put in hot water and is soaked, and stirred, seed is then crumpled during immersion, removal epidermis glues Liquid, control water treats vernalization;3. seed of bitter gourd is placed on refrigerator freezing 2h;4. by the seed of bitter gourd and yellow sand after frost, silkworm excrement, pyrrole effect Grand, lime sulfur, ethephon (CEPHA),2-(chloroethyl) phosphonic acid part are mixed;5. by seed of bitter gourd mixture, it is placed in incubator, bottom pad bamboo mat, top layer lid straw screen or mat, Vernalization is carried out, there is 97% seed dew bud after 5-6 days, complete vernalization.This patent is disadvantageous in that:By the hardship after frost Melon seed and yellow sand, silkworm excrement, forchlorfenuron, lime sulfur, ethephon (CEPHA),2-(chloroethyl) phosphonic acid part are mixed, relatively costly;The seed for having 97% after 5-6 days reveals Bud, germinating time is long, and the regularity that sprouts is poor.
The content of the invention improves balsam pear kind and sprouts present invention aim to provide a kind of efficient germinating method of balsam pear kind Rate and regularity.
In order to achieve the above object, the efficient germinating method of balsam pear kind that the present invention is provided, specifically includes following steps:
1. seed clear water, is soaked into half an hour at normal temperatures, primary vernalization is carried out;
2., seed is put into 60~65 DEG C of hot water, stirring makes seed be heated evenly it, is naturally cooling to 52 DEG C ± 2 DEG C, and constant temperature soaks 10 minutes in 52 DEG C of ± 2 DEG C of hot water, carries out hot and humid vernalization;This step can also realize that primary is gone out Bacterium.
3. after, hot water is naturally cooling to 28~32 DEG C, constant temperature soaks 5 hours, carries out low temperature and high relative humidity vernalization;
4., to take out be put into 0.1% liquor potassic permanganate after seed and soak 5 minutes, sterilized and stimulated seed coat;
5. after, clear water is cleaned, seed is put into the gibberellin aqueous solution of 250ppm concentration and is soaked 6 hours, carry out medicine Vernalization;
6., to fall and cut off seed tip shell with scissors after doing, carry out physics broken shell;This step can not injure bud embryo.
7. it is put into 32 DEG C ± 1 DEG C of insulating box after, seed is placed in the towel of moistening, carries out moisturizing constant temperature and urge Bud;Towel is cheap, with good gas permeability and moisture-retaining capacity.
8., during the vernalization of moisturizing constant temperature, seed-breeding field humidity is checked daily, done and answered moisturizing, and stir seed, enter pedestrian Work vernalization;
9., seed can all sprout for 40--60 hours in insulating box, complete germination process.
The efficient germinating method of balsam pear kind provided by the present invention simultaneously by seed carry out primary vernalization, hot and humid vernalization, Low temperature and high relative humidity vernalization, medicine vernalization, the vernalization of moisturizing constant temperature and artificial germination, bud ratio are up to 100%;Seed is in insulating box Can all sprout within 40--60 hours, germinating time is short, sprouts in good order, it is adaptable to which balsam pear kind is germinateed.
The present invention is further illustrated below according to specific embodiment for specific embodiment, but is not intended to limit Protection scope of the present invention.
Embodiment 1:The efficient germinating method of balsam pear kind, specifically includes following steps:
1. seed clear water, is soaked into half an hour at normal temperatures, primary vernalization is carried out;
2., seed is put into 60 DEG C of hot water, stirring makes seed be heated evenly it, is naturally cooling to 50 DEG C, and 50 Constant temperature soaks 10 minutes in DEG C hot water, carries out hot and humid vernalization;This step can also realize primary sterilizing.
3. after, hot water is naturally cooling to 28 DEG C, constant temperature soaks 5 hours, carries out low temperature and high relative humidity vernalization;
4., to take out be put into 0.1% liquor potassic permanganate after seed and soak 5 minutes, sterilized and stimulated seed coat;
5. after, clear water is cleaned, seed is put into the gibberellin aqueous solution of 250ppm concentration and is soaked 6 hours, carry out medicine Vernalization;
6., to fall and cut off seed tip shell with scissors after doing, carry out physics broken shell;This step can not injure bud embryo.
7. it is put into 32 DEG C ± 1 DEG C of insulating box after, seed is placed in the towel of moistening, carries out moisturizing constant temperature and urge Bud;Towel is cheap, with good gas permeability and moisture-retaining capacity.
8., during the vernalization of moisturizing constant temperature, seed-breeding field humidity is checked daily, done and answered moisturizing, and stir seed, enter pedestrian Work vernalization;
9., seed can all sprout for 40--60 hours in insulating box, complete germination process.
Embodiment 2:The efficient germinating method of balsam pear kind, specifically includes following steps:
1. seed clear water, is soaked into half an hour at normal temperatures, primary vernalization is carried out;
2., seed is put into 61 DEG C of hot water, stirring makes seed be heated evenly it, is naturally cooling to 51 DEG C, and 51 Constant temperature soaks 10 minutes in DEG C hot water, carries out hot and humid vernalization;This step can also realize primary sterilizing.
3. after, hot water is naturally cooling to 34 DEG C, constant temperature soaks 5 hours, carries out low temperature and high relative humidity vernalization;
4., to take out be put into 0.1% liquor potassic permanganate after seed and soak 5 minutes, sterilized and stimulated seed coat;
5. after, clear water is cleaned, seed is put into the gibberellin aqueous solution of 250ppm concentration and is soaked 6 hours, carry out medicine Vernalization;
6., to fall and cut off seed tip shell with scissors after doing, carry out physics broken shell;This step can not injure bud embryo.
7. it is put into 32 DEG C ± 1 DEG C of insulating box after, seed is placed in the towel of moistening, carries out moisturizing constant temperature and urge Bud;Towel is cheap, with good gas permeability and moisture-retaining capacity.
8., during the vernalization of moisturizing constant temperature, seed-breeding field humidity is checked daily, done and answered moisturizing, and stir seed, enter pedestrian Work vernalization;
9., seed can all sprout for 40--60 hours in insulating box, complete germination process.
Embodiment 3:The efficient germinating method of balsam pear kind, specifically includes following steps:
1. seed clear water, is soaked into half an hour at normal temperatures, primary vernalization is carried out;
2., seed is put into 62 DEG C of hot water, stirring makes seed be heated evenly it, is naturally cooling to 52 DEG C, and 52 Constant temperature soaks 10 minutes in DEG C hot water, carries out hot and humid vernalization;This step can also realize primary sterilizing.
3. after, hot water is naturally cooling to 29 DEG C, constant temperature soaks 5 hours, carries out low temperature and high relative humidity vernalization;
4., to take out be put into 0.1% liquor potassic permanganate after seed and soak 5 minutes, sterilized and stimulated seed coat;
5. after, clear water is cleaned, seed is put into the gibberellin aqueous solution of 250ppm concentration and is soaked 6 hours, carry out medicine Vernalization;
6., to fall and cut off seed tip shell with scissors after doing, carry out physics broken shell;This step can not injure bud embryo.
7. it is put into 32 DEG C ± 1 DEG C of insulating box after, seed is placed in the towel of moistening, carries out moisturizing constant temperature and urge Bud;Towel is cheap, with good gas permeability and moisture-retaining capacity.
8., during the vernalization of moisturizing constant temperature, seed-breeding field humidity is checked daily, done and answered moisturizing, and stir seed, enter pedestrian Work vernalization;
9., seed can all sprout for 40--60 hours in insulating box, complete germination process.
Embodiment 4:The efficient germinating method of balsam pear kind, specifically includes following steps:
1. seed clear water, is soaked into half an hour at normal temperatures, primary vernalization is carried out;
2., seed is put into 63 DEG C of hot water, stirring makes seed be heated evenly it, is naturally cooling to 53 DEG C, and 53 Constant temperature soaks 10 minutes in DEG C hot water, carries out hot and humid vernalization;This step can also realize primary sterilizing.
3. after, hot water is naturally cooling to 30 DEG C, constant temperature soaks 5 hours, carries out low temperature and high relative humidity vernalization;
4., to take out be put into 0.1% liquor potassic permanganate after seed and soak 5 minutes, sterilized and stimulated seed coat;
5. after, clear water is cleaned, seed is put into the gibberellin aqueous solution of 250ppm concentration and is soaked 6 hours, carry out medicine Vernalization;
6., to fall and cut off seed tip shell with scissors after doing, carry out physics broken shell;This step can not injure bud embryo.
7. it is put into 32 DEG C ± 1 DEG C of insulating box after, seed is placed in the towel of moistening, carries out moisturizing constant temperature and urge Bud;Towel is cheap, with good gas permeability and moisture-retaining capacity.
8., during the vernalization of moisturizing constant temperature, seed-breeding field humidity is checked daily, done and answered moisturizing, and stir seed, enter pedestrian Work vernalization;
9., seed can all sprout for 40--60 hours in insulating box, complete germination process.
Embodiment 5:The efficient germinating method of balsam pear kind, specifically includes following steps:
1. seed clear water, is soaked into half an hour at normal temperatures, primary vernalization is carried out;
2., seed is put into 64 DEG C of hot water, stirring makes seed be heated evenly it, is naturally cooling to 54 DEG C, and 54 Constant temperature soaks 10 minutes in DEG C hot water, carries out hot and humid vernalization;This step can also realize primary sterilizing.
3. after, hot water is naturally cooling to 31 DEG C, constant temperature soaks 5 hours, carries out low temperature and high relative humidity vernalization;
4., to take out be put into 0.1% liquor potassic permanganate after seed and soak 5 minutes, sterilized and stimulated seed coat;
5. after, clear water is cleaned, seed is put into the gibberellin aqueous solution of 250ppm concentration and is soaked 6 hours, carry out medicine Vernalization;
6., to fall and cut off seed tip shell with scissors after doing, carry out physics broken shell;This step can not injure bud embryo.
7. it is put into 32 DEG C ± 1 DEG C of insulating box after, seed is placed in the towel of moistening, carries out moisturizing constant temperature and urge Bud;Towel is cheap, with good gas permeability and moisture-retaining capacity.
8., during the vernalization of moisturizing constant temperature, seed-breeding field humidity is checked daily, done and answered moisturizing, and stir seed, enter pedestrian Work vernalization;
9., seed can all sprout for 40--60 hours in insulating box, complete germination process.
Embodiment 6:The efficient germinating method of balsam pear kind, specifically includes following steps:
1. seed clear water, is soaked into half an hour at normal temperatures, primary vernalization is carried out;
2., seed is put into 65 DEG C of hot water, stirring makes seed be heated evenly it, is naturally cooling to 52 DEG C, and 52 Constant temperature soaks 10 minutes in DEG C hot water, carries out hot and humid vernalization;This step can also realize primary sterilizing.
3. after, hot water is naturally cooling to 32 DEG C, constant temperature soaks 5 hours, carries out low temperature and high relative humidity vernalization;
4., to take out be put into 0.1% liquor potassic permanganate after seed and soak 5 minutes, sterilized and stimulated seed coat;
5. after, clear water is cleaned, seed is put into the gibberellin aqueous solution of 250ppm concentration and is soaked 6 hours, carry out medicine Vernalization;
6., to fall and cut off seed tip shell with scissors after doing, carry out physics broken shell;This step can not injure bud embryo.
7. it is put into 32 DEG C ± 1 DEG C of insulating box after, seed is placed in the towel of moistening, carries out moisturizing constant temperature and urge Bud;Towel is cheap, with good gas permeability and moisture-retaining capacity.
8., during the vernalization of moisturizing constant temperature, seed-breeding field humidity is checked daily, done and answered moisturizing, and stir seed, enter pedestrian Work vernalization;
9., seed can all sprout for 40--60 hours in insulating box, complete germination process.
, simply be changed for temperature parameter, remaining method by the efficient germinating method of balsam pear kind according to above-described embodiment 1-6 It is identical.Each embodiment carries out Seed germination checking using 100 seed of bitter gourd, records the germination result of each embodiment. Above-described embodiment 1-6 the results are shown in Table 1:
Table 1:The balsam pear kind germination result of embodiment 1-6
Scheme Balsam pear kind quantity Germination initial time The germination termination time Sprout number Do not sprout reason
Embodiment 1 100 46 hours 55 hours 97 The necrosis of bud embryo
Embodiment 2 100 46 hours 58 hours 95 The necrosis of bud embryo
Embodiment 3 100 44 hours 51 hours 98 The necrosis of bud embryo
Embodiment 4 100 45 hours 53 hours 100
Embodiment 5 100 46 hours 55 hours 99 The necrosis of bud embryo
Embodiment 6 100 46 hours 56 hours 98 The necrosis of bud embryo
As seen from Table 1, the seed of bitter gourd germination initial time of embodiment 1-6 and germination termination time are at 40--60 hours Between, germinating time is short, germinating time Relatively centralized, sprouts in good order.Remove bud embryo necrosis situation, the germination of seed of bitter gourd Rate is 100%.
The scope of the present invention is related to all the above version.

Claims (1)

1. a kind of efficient germinating method of balsam pear kind, specifically includes following steps:
1. seed clear water, is soaked into half an hour at normal temperatures, primary vernalization is carried out;
2., seed is put into 60~65 DEG C of hot water, stirring makes seed be heated evenly it, is naturally cooling to 52 DEG C ± 2 DEG C, and Constant temperature soaks 10 minutes in 52 DEG C of ± 2 DEG C of hot water, carries out hot and humid vernalization;This step can also realize primary sterilizing.
3. after, hot water is naturally cooling to 28~32 DEG C, constant temperature soaks 5 hours, carries out low temperature and high relative humidity vernalization;
4., to take out be put into 0.1% liquor potassic permanganate after seed and soak 5 minutes, sterilized and stimulated seed coat;
5. after, clear water is cleaned, seed is put into the gibberellin aqueous solution of 250ppm concentration and is soaked 6 hours, carry out medicine vernalization;
6., to fall and cut off seed tip shell with scissors after doing, carry out physics broken shell;This step can not injure bud embryo.
7. it is put into 32 DEG C ± 1 DEG C of insulating box after, seed is placed in the towel of moistening, carries out moisturizing constant temperature vernalization;Hair Towel is cheap, with good gas permeability and moisture-retaining capacity.
8., during the vernalization of moisturizing constant temperature, seed-breeding field humidity is checked daily, done and answered moisturizing, and stir seed, manually urged Bud;
9., seed can all sprout for 40--60 hours in insulating box, complete germination process.
CN201710045960.3A 2017-01-16 2017-01-16 The efficient germinating method of balsam pear kind Pending CN106797728A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107996067A (en) * 2018-01-17 2018-05-08 安徽省华禾种业有限公司 A kind of method for improving seed of bitter gourd germination percentage
CN109379922A (en) * 2018-11-22 2019-02-26 成都金田种苗有限公司 A kind of germination accelerating method of seed of bitter gourd

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08140477A (en) * 1994-11-16 1996-06-04 Tomoyuki Sumi Production of sprout of efficacy comprising seed of balsam pear as raw material
CN104335782A (en) * 2013-07-30 2015-02-11 郑佩芳 Early-maturing cultivation method of bitter gourds

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08140477A (en) * 1994-11-16 1996-06-04 Tomoyuki Sumi Production of sprout of efficacy comprising seed of balsam pear as raw material
CN104335782A (en) * 2013-07-30 2015-02-11 郑佩芳 Early-maturing cultivation method of bitter gourds

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
段敬杰: "《提高苦瓜商品性栽培技术问答》", 31 July 2013, 金盾出版社 *
饶贵珍: "几种不同处理对苦瓜种子发芽力的影响", 《蔬菜》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107996067A (en) * 2018-01-17 2018-05-08 安徽省华禾种业有限公司 A kind of method for improving seed of bitter gourd germination percentage
CN109379922A (en) * 2018-11-22 2019-02-26 成都金田种苗有限公司 A kind of germination accelerating method of seed of bitter gourd

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