CN1067558C

CN1067558C - Blood-enriching agent-Yixuejing and preparation method thereof

Info

Publication number: CN1067558C
Application number: CN96101823A
Authority: CN
Inventors: 庄庆彬
Original assignee: Individual
Current assignee: Li Yihai
Priority date: 1996-01-16
Filing date: 1996-01-16
Publication date: 2001-06-27
Anticipated expiration: 2016-01-16
Also published as: CN1154851A

Abstract

The present invention relates to a medicinal preparation prepared from vegetable materials and animals' blood and viscera, which is called medicine for benefiting blood. The medicine for benefiting blood is prepared by using chlorhematin as a main ingredient and matching with a plurality of traditional Chinese medicines such as Chinese date, fruit of Chinese wolfberry, cooked rehmannia root, angelica, astragalus root, etc. which can invigorate qi and blood. The medicine for benefiting blood can supplement ferrum and blood for human bodies and promote the synthesis of hemoglobin for enhancing the hematopoietic function of human body selves, thereby achieving the aim of treating both the manifestations and the root causes of iron-deficiency anemia. The medicine for benefiting blood has the advantages of easy acquirement of raw materials, advanced technology and reasonable dosage form.

Description

A kind of hematinic and manufacture method thereof

The present invention relates to a kind of medicinal preparation that derives from vegetable material and animal blood, internal organs.

Iron deficiency anemia is one of modal nutritional disease of child, and developing country's prevalence is 30%～90%, and the average prevalence of China's preschooler is 37%, some areas reach 76%, iron deficiency can make the vigor of body iron enzyme descend, and influences the body homergy, the harm children's health.Therefore, early prevention and treatment in time, favourable child grows up healthy and sound.It is the iron supplement agent of representative that present treatment at iron deficiency anemia mainly contains with the ferrous sulfate, comprise ferrous sulfate, ferrous fumarate, ferric ammonium citrate, Ferrous gluconate etc., but all belong to inorganic mineral ferrum, its common shortcoming is an absorption rate low (being about 5%～10%) in the human body, big to GI irritation, side effect is big, and has the distinctive rust fishy smell of ferrum, and mouthfeel is bad.In therapeutic process, patient Chang Yin can not accept and drug withdrawal.

Hemin (Hemin) is the organic-biological ferrum that is extracted by animal blood, internal organs, its absorption rate height, reach 25%～35%, be about 5 times of ferrous sulfate, from molecular structure, the molecule that haemachrome is nonpolar or polarity is minimum, can infiltrate cell,, and not have GI irritation for body directly utilizes, no rust fishy smell is the efficient iron supplement agent of a new generation.But its composition of iron supplement agent that contains hemin at present is mainly based on Western medicine, and as " the powerful iron oral liquid " that Pingshan, Foochow pharmaceutical factory produces, its component is that hemin, sodium bicarbonate, glycerol, Mel, carbonyl methyl cellulose are received.

Purpose of the present invention aims to provide a kind of organically the combination of Chinese and Western medicine, is Main Ingredients and Appearance with the hemin, but is equipped with the hematinic and the manufacture method thereof of the plurality of Chinese composition composition of benefiting vital QI and blood.

The said hematinic of the present invention is to be made by the raw material of following weight ratio: hemin: Fructus Jujubae: Fructus Lycii: Radix Rehmanniae Preparata: Radix Angelicae Sinensis: the Radix Astragali: brown sugar: dextrin is 0.4: 300: 300: 450: 200: 450: (690～730): (120～150).

The manufacture method of the said hematinic of the present invention is as follows:

1) at first is the extraction of hemin, gets bright animal blood (for example Sanguis Bovis seu Bubali, Sanguis sus domestica etc.), the centrifugal blood clot that gets, add water and make haemolysis, after the repeated hydrogenation sodium oxide is regulated its pH value to 8.5, add protease, in 50 ℃ of hydrolysis 5h, be warming up to 80 ℃ and kill enzyme, be cooled to again below 40 ℃, add 0.1% magnesium chloride solution, make coagulation, add hydrochloric acid and regulate its pH value to 4.0, leave standstill, filter, the filtering residue washing of blunging, refilter,, get the hemin crude product in 70～80 ℃ of drying 4～6h.After the hemin crude product added pyridine dissolving, add chloroform again, close plug jolting after-filtration, filter cake washs with chloroform, merging filtrate, add the glacial acetic acid heated and boiled after, add saturated nacl aqueous solution, concentrated hydrochloric acid solution stirs evenly, filter, use the chloroform washing leaching cake, merging filtrate, leave standstill 12h, refilter, use the glacial acetic acid washing leaching cake, airing gets hemin.

2) Chinese medicine extraction: after by said ratio Radix Rehmanniae Preparata, Radix Angelicae Sinensis, the Radix Astragali, Fructus Jujubae, Fructus Lycii being washed with water, decoct 2 times each 1h respectively with 8～10 times of amounts, 6～8 times of water gagings, merge decoction liquor, filter, be concentrated into the thick paste shape, add equivalent 95% ethanol and place precipitation 24h, filter, filtering residue 50% washing with alcohol is filtered merging filtrate, behind way of distillation recovery ethanol, be concentrated into the thick paste shape.

3) electuary preparation: get hemin, brown powdered sugar, dextrin by proportioning, mix homogeneously, add the Chinese medicine extract thick paste, mixing is a wetting agent with 68%～72% ethanol, make soft material, cross 12 mesh sieves and granulate, 50～60 ℃ of drying 8～10h remove coarse grain through 12 mesh sieve granulate, 55 mesh sieves are removed fine powder, packing.

Hematinic provided by the invention is guidance with China's Traditional health care theory, plurality of Chinese with efficient iron supplement agent hemin and benefiting vital QI and blood is that primary raw material is made, both the timely iron supplement of human body was enriched blood, promoted the synthetic of hemoglobin again, can improve the human body self hemopoietic function, reach purpose, and raw material be easy to get the iron deficiency anemia treating both the principal and secondary aspects of a disease, technology advanced person, dosage form is reasonable.

The invention will be further described below in conjunction with embodiment.

Embodiment 1:

Get bright Sanguis Bovis seu Bubali 10Kg, the centrifugal blood clot 4Kg that gets adds water 10L and stirs haemolysis, adds 1mol/L NaOH20ml and regulates pH value to 8.5, adds proteinase 8 0g, in 50 ℃ of hydrolysis 5h, is warming up to 80 ℃ and kills enzyme 0.5h, is cooled to about 35 ℃ again, adds 0.1%MgCl ₂Solution 100ml makes coagulation, adds 1mol/L HCl32ml accent PH to 4.0, places 2h, filters, and with filtering residue 2250g, adds water 20L and stirs 0.5h, filters, and filtering residue gets hemin crude product 1448g at 70～80 ℃ of dry 5h.After crude product added 7240ml pyridine dissolving, add chloroform 10L, close plug jolting 20min filters, filter cake washs with the 1000ml chloroform, and merging filtrate adds glacial acetic acid 6500ml, after the heated and boiled, add the 900ml saturated nacl aqueous solution, the 900ml concentrated hydrochloric acid solution, stir evenly, cross Hu, with 1000ml chloroform washing leaching cake, merging filtrate leaves standstill 12h, refilters, with 7000ml glacial acetic acid washing leaching cake, airing, get hemin 44.7g (measuring purity is 86.72%).

After getting Fructus Jujubae 3000g, Fructus Lycii 3000g, Radix Rehmanniae Preparata 4500g, Radix Angelicae Sinensis 2000g, Radix Astragali 4500g and washing with water, Fructus Jujubae breaks, and decocts 2 times with 10 times of amounts, 7 times of water gagings respectively, each 1h merges decoction liquor, is concentrated into the thick paste shape after the filtration, add equivalent 95% ethanol, place precipitation 24h, filter, filtering residue 50% washing with alcohol, filter, merging filtrate is behind way of distillation recovery ethanol, be concentrated into the thick paste shape, get thick paste 2150g.

Take by weighing the 1200g dextrin, 7290g brown sugar is standby, gets above-mentioned hemin 4.61g, pulverizes 120 mesh sieves, add brown powdered sugar to 640g by the equivalent incremental method, mixing adds dextrin and remaining brown powdered sugar, mix homogeneously adds the Chinese medicine extract thick paste, mixes about 10min, add 70% ethanol 1600ml again, make soft material after the mixing, cross 12 mesh sieves and granulate, in 55 ℃ of dry 9h, remove coarse grain through 12 mesh sieve granulate, 55 mesh sieves are removed fine powder, the packing of weighing.

Embodiment 2:

Get Sanguis sus domestica 60.5Kg, get hemin 283.91g by the extracting method of embodiment 1 hemin, mensuration content is 84.53g.

Get Fructus Jujubae 3000g, Fructus Lycii 3000g, Radix Rehmanniae Preparata 4500g, Radix Angelicae Sinensis 2000g, Radix Astragali 4500g, produce thick paste by embodiment 1 Chinese medicine extraction method.

Get dextrin 1500g, brown sugar 6931.6g, above-mentioned hemin 4.74g pulverized 120 mesh sieves, added brown powdered sugar to 568g by the equivalent incremental method, mixing, add dextrin and remaining brown powdered sugar, add the Chinese medicine extract thick paste behind the mix homogeneously, add 70% ethanol 1800ml again behind the mixing 10min, make soft material after the mixing, crossing 12 mesh sieves granulates, in 60 ℃ of dry 8h, last granulate, packing.

Claims

1. hematinic, contain hemin, it is characterized in that it is to be made by the raw material of following weight ratio: hemin: Fructus Jujubae: Fructus Lycii: Radix Rehmanniae Preparata: Radix Angelicae Sinensis: the Radix Astragali: brown sugar: dextrin is 0.4: 300: 300: 450: 200: 450: (690～730): (120～150).

2. the manufacture method of a hematinic is characterized in that:

1) extraction of hemin: get bright animal blood, the centrifugal blood clot that gets adds water and makes haemolysis, after the repeated hydrogenation sodium oxide is regulated its pH value to 8.5, add protease, in 50 ℃ of hydrolysis 5h, be warming up to 80 ℃ and kill enzyme, be cooled to again below 40 ℃, add 0.1% magnesium chloride solution and make coagulation, add hydrochloric acid and regulate its pH value to 4.0, leave standstill, filter, the filtering residue washing of blunging, refilter,, get the hemin crude product in 70～80 ℃ of drying 4～6h; After the hemin crude product added pyridine dissolving, add chloroform, close plug jolting after-filtration, filter cake washs with chloroform, merging filtrate, add the glacial acetic acid heated and boiled after, add saturated nacl aqueous solution, concentrated hydrochloric acid solution stirs evenly, filter, use the chloroform washing leaching cake, merging filtrate, leave standstill 12h, refilter, use the glacial acetic acid washing leaching cake, airing gets hemin;

2) Chinese medicine extraction: after by proportioning Radix Rehmanniae Preparata, Radix Angelicae Sinensis, the Radix Astragali, Fructus Jujubae, Fructus Lycii being washed with water, decoct 2 times each 1h respectively with 8～10 times of amounts, 6～8 times of water gagings, merge decoction liquor, filter, be concentrated into the thick paste shape, add equivalent 95% ethanol and place precipitation 24h, filter, filtering residue 50% washing with alcohol is filtered merging filtrate, behind way of distillation recovery ethanol, be concentrated into the thick paste shape;

3) electuary preparation: get hemin, brown powdered sugar, dextrin by proportioning, mix homogeneously, add the Chinese medicine extract thick paste, mixing is a wetting agent with 68%～72% ethanol, make soft material, cross 12 mesh sieves and granulate, 50～60 ℃ of drying 8～10h remove coarse grain through 12 mesh sieve granulate, 55 mesh sieves are removed fine powder, packing;

4) weight ratio of each raw material is: hemin: Fructus Jujubae: Fructus Lycii: Radix Rehmanniae Preparata: Radix Angelicae Sinensis: the Radix Astragali: brown sugar: dextrin is 0.4: 300: 300: 450: 200: 450: (690～730): (120～150).

3. the manufacture method of a kind of hematinic as claimed in claim 2 is characterized in that described bright animal blood is bright Sanguis Bovis seu Bubali, fresh porcine blood.