CN106748508A - A kind of application based on Kiwi berry root endogeny rayungus and its in tobacco organic fertilizer - Google Patents
A kind of application based on Kiwi berry root endogeny rayungus and its in tobacco organic fertilizer Download PDFInfo
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Abstract
The present invention provides a kind of tobacco organic fertilizer based on Kiwi berry root endogeny rayungus and preparation method thereof, the tobacco organic fertilizer includes tobacco compound fertilizer, vinasse composite fertilizer and based on Kiwi berry root endogeny rayungus, tobacco compound fertilizer includes phosphorous acid sylvite, phosphorous acid ammonium salt, boron fertilizer and available nitrogen phosphate fertilizer, vinasse composite fertilizer is using vinasse solid content as raw material, through microorganism aerobic fermentation, endoplasm is allocated, drying sterilization is obtained, it is the styreptomyces globispotus strain that the Kiwi berry root by suffering from canker prepares as raw material based on Kiwi berry root endogeny rayungus, three is well mixed, form the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus.Tobacco organic fertilizer prepared by the present invention belongs to full unit's biological organic fertilizer, component by weight percentage, including：Inorganic nutrients 10 15%, amino acid 2 3%, organic matter 40 45% and moisture 25%, the bacterial content of actinomyces is 2 × 107Individual/g, pH are 68.
Description
Technical field
The invention belongs to biological organic fertilizer field of material technology, and in particular to one kind is based on Kiwi berry root endogeny rayungus
And its application in tobacco organic fertilizer.
Background technology
Full unit's bio-organic fertilizer refers to integrating the new of organic fertilizer, chemical fertilizer, amino acid fertilizer and functional microorganism
Bio-organic fertilizer, belongs to third generation microbial manure, compound with nutrition, conditioning, the fertile medicine of plant protection three-in-one and acting type
Microbial manure.Full unit's bio-organic fertilizer compared with microbial bacterial agent, composite microbiological fertilizer and biological organic fertilizer, microorganism
Microbial inoculum overemphasizes microorganism fungus kind, does not have organic matter and nutrient, it is difficult to external source functional microorganism is colonized in soil；
Composite microbiological fertilizer, the content of organic matter is too low, inorganic nutrients no ceiling, nutrient content be easily able to 20% or even 30% with
On, it is unfavorable for that the vigor of function bacterium in product is preserved, field efficacy is that inorganic composite mixed fertilizer produces biology actually by inorganic nutrients
Organic fertilizer overemphasizes organic matter without inorganic nutrients index, and the effect to this season crop especially plant growth early stage is failed to understand
It is aobvious.Therefore, full unit's bio-organic fertilizer is a kind of low-carbon green environmental protection product, and sustainable supply nutrient, to increase soil organic
Matter, improve and soil texture and increase soil fertility, especially controlling and overcoming because being excessively used chemical fertilizer for a long time and agricultural chemicals causes
Soil continuous cropping obstacle, reducing residues of pesticides aspect has unique effect.
New flue-cured tobacco varieties cloud and mist 87 is that Chinese tobacco breeding research (south) center, Yunnan Inst. of Tobacco Sciences agricultural are ground
Study carefully selected country's self-fertile kind cloud and mist 2 and make maternal, U.S. introduced variety K326 is paternal hybrid, is formed through pedigree seed selection,
How golden yellow obtain former cigarette, colourity is strong, and oil is more, short texture, the moderate high-quality of vane thickness, disease-resistant, easily roasting, adaptability
By force, fragrance and jealous good new varieties.The anti-balck shank of new flue-cured tobacco varieties cloud and mist 87, in anti-bridegroom's or husband's side root knot nematode disease, resistance to common flower
Leaf disease, disease-resistanting leaf spot, middle resistance to bacterial wilt, cloud and mist 87 transplants slow-growing to prosperous long-term cigarette strain, and late growing stage is rapid, grows whole
Together, the more resistance to fertilizer of kind, in the course of cultivation, fertilizer requirement is big.One kind disclosed in Chinese patent CN 105367169A is exclusively used in cloud and mist
87 examine cigarette organic-inorganic compound mixed fertilizer, this examine cigarette organic-inorganic compound mixed fertilizer including cake fertilizer, barnyard manure, microbial bacterial agent, ammonium nitrate phosphorus,
Potassium sulfate, potassium nitrate and general calcium, mix by by various fertilizer, improve the utilization rate of Nitrogen by Flue-Cured Tobacco, reduce the use of chemical fertilizer
Rate, improves the quality and economic benefits of flue-cured tobacco.It follows that persistently and fertility can be suppressed into the fertilizer of soil acidification to cloud and mist 87
Applied fertilizer, the sustainable development of cloud and mist 87 could be promoted, therefore complete first biological organic fertilizer is used for ten in the cultivation of cloud and mist 87
Divide necessity.
The content of the invention
The technical problem to be solved in the present invention is to provide one kind based on Kiwi berry root endogeny rayungus and its has in tobacco
Application in machine fertilizer, including tobacco compound fertilizer, vinasse composite fertilizer and Kiwi berry root endogeny rayungus, the tobacco organic fertilizer of preparation
Can be used as the base manure of tobacco or additional fertilizer, it is also possible to other composite fertilizers, bactericide, insecticide compound use, there is provided balanced
Comprehensively influence, the fertility of persistence raising soil, and then the yield and quality of tobacco is improved, it is good in economic efficiency, and to environment
Burden is small, and resource utilization is high.
In order to solve the above technical problems, the technical scheme is that：
A kind of tobacco organic fertilizer based on Kiwi berry root endogeny rayungus, it is characterised in that described based on Chinese actinidia root
The tobacco organic fertilizer of portion's endogeny rayungus includes tobacco compound fertilizer, vinasse composite fertilizer and Kiwi berry root endogeny rayungus, described
Kiwi berry root endogeny rayungus are the styreptomyces globispotus strain that the Kiwi berry root by suffering from canker prepares as raw material, the tobacco
Nitrogen, phosphorus pentoxide, the mass percentage content of potassium oxide are 10 in composite fertilizer:20:20, nitrogen, five in the vinasse composite fertilizer
Aoxidize two phosphorus, the mass percentage content of potassium oxide is 2:1:2.
Used as the preferred of above-mentioned technical proposal, the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus can be with
Other composite fertilizers, bactericide, insecticide compound use, the group of the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus
Point, by weight percentage, including：Inorganic nutrients 10-15%, amino acid 2-3%, organic matter 40-45% and moisture 25%, institute
The content for stating actinomyces bacterium is 2 × 107Individual/g, pH are 6-8.
Used as the preferred of above-mentioned technical proposal, the tobacco compound fertilizer includes phosphorous acid sylvite, phosphorous acid ammonium salt, boron fertilizer
With available nitrogen phosphate fertilizer.
Used as the preferred of above-mentioned technical proposal, the vinasse composite fertilizer is using vinasse solid content as raw material, through microorganism
Aerobic fermentation, endoplasm allotment, drying sterilization are obtained, and the leavening of so-called microorganism aerobic fermentation is cellulose, digestive enzyme, albumen
Catabolic enzyme or root enzyme, the endoplasm blender of the endoplasm allotment is Freamine Ⅲ.
The present invention also provides a kind of preparation method of the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus, its feature
It is to comprise the following steps：
(1) the Kiwi berry root samples of canker are suffered from collection, are cleaned with the Tween-20 solution that mass percent is 0.1%
30s, aseptic water washing 3 times, 75% ethanol immersion 5min, aseptic water washing 3 times, sodium chlorate solution's leaching of available chlorine content 2%
Bubble 3-5min, aseptic water washing 3 times, mass fraction be 10% sodium bicarbonate solution immersion 10min, aseptic water washing 3 times, so
After grind to form homogenate, be connected in TSA culture medium flat plates, cultivate 30d at 28 DEG C, form the culture medium of Kiwi berry root；
(2) extracting single bacterium colony in the culture medium of the Kiwi berry root for preparing step (1) carries out line purifying, separates, and obtains
To based on Kiwi berry root endogeny rayungus；
(3) by step (2) prepare based on Kiwi berry root endogeny rayungus activation after be inoculated in Gause I nutrient solution
In, 30 DEG C, 96h is cultivated in the constant-temperature table of 120r/min, as seed liquor with 1:1 ratio adds Gause I culture medium, 30
DEG C, 96h is cultivated in the constant-temperature table of 120r/min, its viable count is reached more than 1,000,000,000, obtain raw unwrapping wire in Kiwi berry root
Fermented liquid；
(4) the Kiwi berry root endogeny rayungus zymotic fluid for preparing step (3) dilutes 50-200 times, and to obtain actinomyces dilute
Liquid is released, tobacco compound fertilizer, vinasse composite fertilizer is added, is well mixed, forming the tobacco based on Kiwi berry root endogeny rayungus has
Machine fertilizer.
As the preferred of above-mentioned technical proposal, it is characterised in that the inoculative proportion of the step (1) is 0.5%.
As the preferred of above-mentioned technical proposal, it is characterised in that the standard of the line purifying of the step (2) is according to bacterium
Fall the performance characteristic picking single bacterium colony such as size, form and color and luster.
As the preferred of above-mentioned technical proposal, it is characterised in that the Kiwi berry root endogeny rayungus pair of the step (2)
The withered and yellow germ of staphylococcus aureus, Escherichia coli, pseudomonas syringae, Rhizoctonia solani Kahn, watermelon and tomato early blight bacterium
Inhibited, IAA yield is more than 40mg/L, can produce cellulase.
Used as the preferred of above-mentioned technical proposal, the tobacco based on Kiwi berry root endogeny rayungus of the step (4) has
The mass ratio of tobacco compound fertilizer, vinasse composite fertilizer and actinomyces dilution is 20 in machine fertilizer:50-100:1.
Used as the preferred of above-mentioned technical proposal, the tobacco based on Kiwi berry root endogeny rayungus of the step (4) has
Machine fertilizer can be 50-100kg/ mus as the base manure of tobacco or additional fertilizer, usage amount.
Compared with prior art, the invention has the advantages that：
(1) the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus prepared by the present invention is full unit's biological organic fertilizer,
Including tobacco compound fertilizer, vinasse composite fertilizer and Kiwi berry root endogeny rayungus, it is to avoid microbial bacterial agent, complex microorganism occur
The drawbacks of fertilizer and biological organic fertilizer excessively emphasize single component, can provide balanced comprehensively shadow since the early stage of plant growth
Ring, it is ensured that the nutrient required for plant growth is good, Blumea balsamifera crop can be met on the basis of using a small amount of organic fertilizer
Nutrient demand, significantly inhibits soil acidification, and Effective Regulation edaphon system, persistence improves the fertility of soil, Jin Erti
The yield and quality of tobacco high, good in economic efficiency and small to environmental pressure, resource utilization is high.
(2) the Kiwi berry root contained in the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus prepared by the present invention
Endogeny rayungus are styreptomyces globispotus strain, and styreptomyces globispotus strain can not only promote the growth of tobacco, can also always tobacco plant disease
Fungal pathogenses and the growth of bacterium, with highly effective cellulose degradation capability, can also improve soil physico-chemical property, it is seen that know reductionization
The utilization rate of fertilizer and agricultural chemicals is learned, and without worrying soil acidification, the fertility of raising soil that then can be permanent, green safety
Environmental protection.
(3) tobacco compound fertilizer contained in the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus prepared by the present invention
Including phosphorous acid sylvite, phosphorous acid ammonium salt, boron fertilizer and available nitrogen phosphate fertilizer, the soil-borne disease during tobacco planting can be prevented and treated,
Boron make-up, nitrogen, phosphorus and potassium nutrition, have positive effect to the raising of the yield and quality of tobacco.
(4) the vinasse composite fertilizer contained in the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus prepared by the present invention
It is the organic fertilizer containing amino acid, the content of organic matter is high, nutritious, is conducive to improving soil texture, and fertility instant effect.
(5) present invention prepare the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus can as tobacco base manure,
Can be used as additional fertilizer, it is also possible to which with other composite fertilizers, bactericide, insecticide compound use, application method is simple, controllable
Property is strong.
Specific embodiment
Describe the present invention in detail below in conjunction with specific embodiment, herein illustrative examples of the invention and explanation
It is for explaining the present invention but not as a limitation of the invention.
In middle torrid zone moistening monsoon climate, whole year has a moderate climate, and abundant rainfall, illumination is suitable, and frost-free period is long, the four seasons point
Bright, rain heat is tested with the area in season.Random district's groups dispensing, each cell is used to plant 40 plants of flue-cured tobacco clouds and mists 87 in field,
The flue-cured tobacco cloud and mist 87 with conventional base fertilizer is exercised as isolation strip with 2 between each cell.
Railway carriage or compartment is oriented out according to illumination and wind direction simultaneously, railway carriage or compartment direction is opened unanimously, width is consistent, by unified direction, unification
Highly, 1.2 meters of line-spacing, obstruct high 0.3 meter, accomplish that carriage body is full, trapezoidal, while holding gutter successfully, it is ensured that draining is unimpeded.
Embodiment 1：
(1) the Kiwi berry root samples of canker are suffered from collection, are cleaned with the Tween-20 solution that mass percent is 0.1%
30s, aseptic water washing 3 times, 75% ethanol immersion 5min, aseptic water washing 3 times, sodium chlorate solution's leaching of available chlorine content 2%
Bubble 3min, aseptic water washing 3 times, mass fraction be 10% sodium bicarbonate solution immersion 10min, aseptic water washing 3 times, then
Homogenate is ground to form, is connected in TSA culture medium flat plates according to 0.5% inoculative proportion, 30d is cultivated at 28 DEG C, form Kiwi berry
The culture medium of root.
(2) by the culture medium of Kiwi berry root according to the performance characteristic picking single bacterium colony such as bacterium colony size, form and color and luster
Line purifying is carried out, is separated, obtained based on Kiwi berry root endogeny rayungus.
(3) it is inoculated in Gause I nutrient solution after being activated based on Kiwi berry root endogeny rayungus, 30 DEG C, 120r/
96h is cultivated in the constant-temperature table of min, as seed liquor with 1:1 ratio addition Gause I culture medium, 30 DEG C, 120r/min's
96h is cultivated in constant-temperature table, its viable count is reached more than 1,000,000,000, obtain Kiwi berry root endogeny rayungus zymotic fluid.
(4) Kiwi berry root endogeny rayungus zymotic fluid is diluted 50 times and obtains actinomyces dilution, in tobacco compound fertilizer
Including 5% phosphorous acid sylvite, 3% phosphorous acid ammonium salt, 3% boron fertilizer and available nitrogen phosphate fertilizer, wherein nitrogen, phosphorus pentoxide,
The mass percentage content of potassium oxide is 10:20:20, total nutrient content >=55%, nitrogen in vinasse composite fertilizer, phosphorus pentoxide,
The mass percentage content of potassium oxide is 2:1:2, the content of organic matter >=45%, N-P-K content >=5% is according to mass ratio
20:50:1, tobacco compound fertilizer, vinasse composite fertilizer and the actinomyces dilution that will be added are well mixed, and are formed and are based on Chinese actinidia root
The tobacco organic fertilizer of portion's endogeny rayungus, the component of the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus, by weight hundred
Divide than meter, including：Inorganic nutrients 10%, amino acid 2%, organic matter 40% and moisture 25%, content >=2 of actinomyces bacterium ×
109Cfu/g, pH are 6.
(5) application process：50 kgs/acre of the tobacco organic fertilizer of Kiwi berry root endogeny rayungus will be based on for base fertilizer is applied
And fully stirred evenly with nest soil, then 25 kgs/acre of the tobacco organic fertilizer of Kiwi berry root endogeny rayungus will be based on as topdressing
Applied when taking off railway carriage or compartment on film, after transplanting in 20 days, 25 kilograms of tobacco organic fertilizers based on Kiwi berry root endogeny rayungus have been watered
500 kilograms of pouring roots, every plant of tank 400ml, mulch film is optimal from the PE films of thickness 0.14s (mm).
Embodiment 2：
(1) the Kiwi berry root samples of canker are suffered from collection, are cleaned with the Tween-20 solution that mass percent is 0.1%
30s, aseptic water washing 3 times, 75% ethanol immersion 5min, aseptic water washing 3 times, sodium chlorate solution's leaching of available chlorine content 2%
Bubble 5min, aseptic water washing 3 times, mass fraction be 10% sodium bicarbonate solution immersion 10min, aseptic water washing 3 times, then
Homogenate is ground to form, is connected in TSA culture medium flat plates according to 0.5% inoculative proportion, 30d is cultivated at 28 DEG C, form Kiwi berry
The culture medium of root.
(2) by the culture medium of Kiwi berry root according to the performance characteristic picking single bacterium colony such as bacterium colony size, form and color and luster
Line purifying is carried out, is separated, obtained based on Kiwi berry root endogeny rayungus.
(3) it is inoculated in Gause I nutrient solution after being activated based on Kiwi berry root endogeny rayungus, 30 DEG C, 120r/
96h is cultivated in the constant-temperature table of min, as seed liquor with 1:1 ratio addition Gause I culture medium, 30 DEG C, 120r/min's
96h is cultivated in constant-temperature table, its viable count is reached more than 1,000,000,000, obtain Kiwi berry root endogeny rayungus zymotic fluid.
(4) Kiwi berry root endogeny rayungus zymotic fluid is diluted 200 times and obtains actinomyces dilution, in tobacco compound fertilizer
Including 5% phosphorous acid sylvite, 3% phosphorous acid ammonium salt, 3% boron fertilizer and available nitrogen phosphate fertilizer, wherein nitrogen, phosphorus pentoxide,
The mass percentage content of potassium oxide is 10:20:20, total nutrient content >=55%, nitrogen in vinasse composite fertilizer, phosphorus pentoxide,
The mass percentage content of potassium oxide is 2:1:2, the content of organic matter >=45%, N-P-K content >=5% is according to mass ratio
20:100:1, tobacco compound fertilizer, vinasse composite fertilizer and the actinomyces dilution that will be added are well mixed, and are formed and are based on Kiwi berry
The tobacco organic fertilizer of root endogeny rayungus, the component of the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus, by weight
Percentages, including：Inorganic nutrients 15%, amino acid 3%, organic matter 45% and moisture 25%, content >=2 of actinomyces bacterium ×
109Cfu/g, pH are 8.
(5) application process：100 kgs/acre of the tobacco organic fertilizer of Kiwi berry root endogeny rayungus will be based on for base fertilizer is applied
Enter and fully stirred evenly with nest soil, then 25 kgs/acre of the tobacco organic fertilizer of Kiwi berry root endogeny rayungus will be based on as topdressing
Applied when railway carriage or compartment on film is taken off, after transplanting in 25 days, 25 kilograms of tobacco organic fertilizers based on Kiwi berry root endogeny rayungus have been converted
500 kilograms of pouring roots of water, every plant of tank 400ml, mulch film is optimal from the PE films of thickness 0.14s (mm).
Embodiment 3：
(1) the Kiwi berry root samples of canker are suffered from collection, are cleaned with the Tween-20 solution that mass percent is 0.1%
30s, aseptic water washing 3 times, 75% ethanol immersion 5min, aseptic water washing 3 times, sodium chlorate solution's leaching of available chlorine content 2%
Bubble 4min, aseptic water washing 3 times, mass fraction be 10% sodium bicarbonate solution immersion 10min, aseptic water washing 3 times, then
Homogenate is ground to form, is connected in TSA culture medium flat plates according to 0.5% inoculative proportion, 30d is cultivated at 28 DEG C, form Kiwi berry
The culture medium of root.
(2) by the culture medium of Kiwi berry root according to the performance characteristic picking single bacterium colony such as bacterium colony size, form and color and luster
Line purifying is carried out, is separated, obtained based on Kiwi berry root endogeny rayungus.
(3) it is inoculated in Gause I nutrient solution after being activated based on Kiwi berry root endogeny rayungus, 30 DEG C, 120r/
96h is cultivated in the constant-temperature table of min, as seed liquor with 1:1 ratio addition Gause I culture medium, 30 DEG C, 120r/min's
96h is cultivated in constant-temperature table, its viable count is reached more than 1,000,000,000, obtain Kiwi berry root endogeny rayungus zymotic fluid.
(4) Kiwi berry root endogeny rayungus zymotic fluid is diluted 100 times and obtains actinomyces dilution, in tobacco compound fertilizer
Including 5% phosphorous acid sylvite, 3% phosphorous acid ammonium salt, 3% boron fertilizer and available nitrogen phosphate fertilizer, wherein nitrogen, phosphorus pentoxide,
The mass percentage content of potassium oxide is 10:20:20, total nutrient content >=55%, nitrogen in vinasse composite fertilizer, phosphorus pentoxide,
The mass percentage content of potassium oxide is 2:1:2, the content of organic matter >=45%, N-P-K content >=5% is according to mass ratio
20:80:1, tobacco compound fertilizer, vinasse composite fertilizer and the actinomyces dilution that will be added are well mixed, and are formed and are based on Chinese actinidia root
The tobacco organic fertilizer of portion's endogeny rayungus, the component of the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus, by weight hundred
Divide than meter, including：Inorganic nutrients 13%, amino acid 2.5%, organic matter 43% and moisture 25%, content >=2 of actinomyces bacterium ×
109Cfu/g, pH are 7.
(5) application process：80 kgs/acre of the tobacco organic fertilizer of Kiwi berry root endogeny rayungus will be based on for base fertilizer is applied
And fully stirred evenly with nest soil, then 25 kgs/acre of the tobacco organic fertilizer of Kiwi berry root endogeny rayungus will be based on as topdressing
Applied when taking off railway carriage or compartment on film, after transplanting in 22 days, 25 kilograms of tobacco organic fertilizers based on Kiwi berry root endogeny rayungus have been watered
500 kilograms of pouring roots, every plant of tank 400ml, mulch film is optimal from the PE films of thickness 0.14s (mm).
Comparative example 1：
By 50 kgs/acre of tobacco compound fertilizer for base fertilizer is applied and is fully stirred evenly with nest soil, then by 25 kilograms of tobacco compound fertilizer/
Mu applied when railway carriage or compartment on film is taken off as topdressing, and after transplanting in 20-25 days, 25 kilograms of tobacco compound fertilizers is watered into 500 kilograms of pouring roots,
Every plant of tank 400ml, mulch film is optimal from the PE films of thickness 0.14s (mm).
Comparative example 2：
By 50 kgs/acre of vinasse composite fertilizer for base fertilizer is applied and is fully stirred evenly with nest soil, then by 25 kilograms of vinasse composite fertilizer/
Mu applied when railway carriage or compartment on film is taken off as topdressing, and after transplanting in 20-25 days, 25 kilograms of vinasse composite fertilizers is watered into 500 kilograms of pouring roots,
Every plant of tank 400ml, mulch film is optimal from the PE films of thickness 0.14s (mm).
Comparative example 3：
By 50 kgs/acre of tobacco compound fertilizer, 50 kgs/acre of vinasse composite fertilizer for base fertilizer is applied and is fully stirred evenly with nest soil,
Again using vinasse composite fertilizer 25 kgs/acre applied when railway carriage or compartment on film is taken off as being topdressed, after transplanting in 20-25 days, by 25 kilograms of vinasse
Composite fertilizer is watered 500 kilograms of pouring roots, and every plant of tank 400ml, mulch film is optimal from the PE films of thickness 0.14s (mm).
Comparative example 4：
By 50 kgs/acre of tobacco compound fertilizer, 50 kgs/acre of vinasse composite fertilizer for base fertilizer is applied and is fully stirred evenly with nest soil,
Again using tobacco compound fertilizer 25 kgs/acre applied when railway carriage or compartment on film is taken off as being topdressed, after transplanting in 20-25 days, by 25 kilograms of tobaccos
Composite fertilizer is watered 500 kilograms of pouring roots, and every plant of tank 400ml, mulch film is optimal from the PE films of thickness 0.14s (mm).
Comparative example 5：
Do not apply fertilizer.After testing, the plant height of pinching of cloud and mist 87 prepared by embodiment 1-3 and comparative example 1-5, leaves number,
The result of yield and nicotine average content is as follows：
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Comparative example 5 | |
| Plant height of pinching (cm) | 118 | 119.5 | 119 | 116 | 115 | 116.5 | 117 | 113 |
| Leaves number (piece) | 28 | 29 | 29 | 26 | 26 | 27 | 28 | 25 |
| Nicotine average content (%) | 2.45 | 2.56 | 2.51 | 2.2 | 2.25 | 2.3 | 2.4 | 2.1 |
| Yield (kg/hm2) | 2936 | 2993 | 2965 | 2716 | 2774 | 2796 | 2812 | 2516 |
As seen from the above table, the plant height of pinching of cloud and mist 87 prepared by the present invention, leaves number, yield and nicotine average content are equal
Significantly improve.
The above-described embodiments merely illustrate the principles and effects of the present invention, not for the limitation present invention.It is any ripe
The personage for knowing this technology all can carry out modifications and changes under without prejudice to spirit and scope of the invention to above-described embodiment.Cause
This, those of ordinary skill in the art is complete with institute under technological thought without departing from disclosed spirit such as
Into all equivalent modifications or change, should be covered by claim of the invention.
Claims (10)
1. a kind of tobacco organic fertilizer based on Kiwi berry root endogeny rayungus, it is characterised in that：It is described based on Kiwi berry root
The tobacco organic fertilizer of endogeny rayungus includes tobacco compound fertilizer, vinasse composite fertilizer and Kiwi berry root endogeny rayungus, the Mi
Monkey peach root endogeny rayungus are the styreptomyces globispotus strains that the Kiwi berry root by suffering from canker prepares as raw material, and the tobacco is answered
Nitrogen, phosphorus pentoxide, the mass percentage content of potassium oxide are 10 in Hefei:20:20, nitrogen, five oxygen in the vinasse composite fertilizer
Change two phosphorus, the mass percentage content of potassium oxide is 2:1:2.
2. a kind of tobacco organic fertilizer based on Kiwi berry root endogeny rayungus according to claim 1, it is characterised in that：
The tobacco organic fertilizer based on Kiwi berry root endogeny rayungus can be with other composite fertilizers, bactericide, insecticide be compound makes
With, the component of the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus, by weight percentage, including：It is inorganic to support
Divide 10-15%, amino acid 2-3%, organic matter 40-45% and moisture 25%, the content of the actinomyces bacterium is 2 × 107Individual/g,
PH is 6-8.
3. a kind of tobacco organic fertilizer based on Kiwi berry root endogeny rayungus according to claim 1, it is characterised in that：
The tobacco compound fertilizer includes phosphorous acid sylvite, phosphorous acid ammonium salt, boron fertilizer and available nitrogen phosphate fertilizer.
4. a kind of tobacco organic fertilizer based on Kiwi berry root endogeny rayungus according to claim 1, it is characterised in that：
The vinasse composite fertilizer is, using vinasse solid content as raw material, to be obtained through microorganism aerobic fermentation, endoplasm allotment, drying sterilization,
The leavening of so-called microorganism aerobic fermentation is cellulose, digestive enzyme, protease or root enzyme, and it is interior that the endoplasm is allocated
Matter blender is Freamine Ⅲ.
5. a kind of preparation method of the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus, it is characterised in that including following
Step：
(1) the Kiwi berry root samples of canker are suffered from collection, and 30s is cleaned with the Tween-20 solution that mass percent is 0.1%,
Aseptic water washing 3 times, 75% ethanol immersion 5min, aseptic water washing 3 times, sodium chlorate solution's immersion 3- of available chlorine content 2%
5min, aseptic water washing 3 times, mass fraction is 10% sodium bicarbonate solution immersion 10min, aseptic water washing 3 times, Ran Houyan
Homogenate is worn into, is connected in TSA culture medium flat plates, 30d is cultivated at 28 DEG C, form the culture medium of Kiwi berry root；
(2) extracting single bacterium colony in the culture medium of the Kiwi berry root for preparing step (1) carries out line purifying, separates, and obtains Mi
Monkey peach root endogeny rayungus；
(3) it is inoculated in Gause I nutrient solution after the Kiwi berry root endogeny rayungus activation for preparing step (2), 30 DEG C,
96h is cultivated in the constant-temperature table of 120r/min, as seed liquor with 1:1 ratio adds Gause I culture medium, 30 DEG C, 120r/
96h is cultivated in the constant-temperature table of min, its viable count is reached more than 1,000,000,000, obtain Kiwi berry root endogeny rayungus zymotic fluid；
(4) the Kiwi berry root endogeny rayungus zymotic fluid for preparing step (3) dilutes 50-200 times and obtains actinomyces dilution,
Tobacco compound fertilizer, vinasse composite fertilizer are added, is well mixed, form the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus.
6. the preparation method of a kind of tobacco organic fertilizer based on Kiwi berry root endogeny rayungus according to claim 5,
It is characterized in that：The inoculative proportion of the step (1) is 0.5%.
7. the preparation method of a kind of tobacco organic fertilizer based on Kiwi berry root endogeny rayungus according to claim 5,
It is characterized in that：The standard of the line purifying of the step (2) is according to the performance characteristic picking such as bacterium colony size, form and color and luster
Single bacterium colony.
8. the preparation method of a kind of tobacco organic fertilizer based on Kiwi berry root endogeny rayungus according to claim 5,
It is characterized in that：The Kiwi berry root endogeny rayungus of the step (2) are false to staphylococcus aureus, Escherichia coli, cloves
The withered and yellow germ of monad, Rhizoctonia solani Kahn, watermelon and tomato early blight bacterium are inhibited, and IAA yield is more than 40mg/L,
Cellulase can be produced.
9. the preparation method of a kind of tobacco organic fertilizer based on Kiwi berry root endogeny rayungus according to claim 5,
It is characterized in that：Tobacco compound fertilizer, vinasse in the tobacco organic fertilizer based on Kiwi berry root endogeny rayungus of the step (4)
The mass ratio of composite fertilizer and actinomyces dilution is 20:50-100:1.
10. the preparation method of a kind of tobacco organic fertilizer based on Kiwi berry root endogeny rayungus according to claim 5,
It is characterized in that：The tobacco organic fertilizer based on Kiwi berry root endogeny rayungus of the step (4) can be used as the base manure of tobacco
Or additional fertilizer, usage amount is 50-100kg/ mus.
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| CN110903136A (en) * | 2019-12-30 | 2020-03-24 | 中盐安徽红四方肥业股份有限公司 | Microbial fertilizer and preparation method thereof |
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| CN110903136A (en) * | 2019-12-30 | 2020-03-24 | 中盐安徽红四方肥业股份有限公司 | Microbial fertilizer and preparation method thereof |
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