CN106725977A - Occupational exposure to magnetic fields experiment rat single cage fixing device - Google Patents
Occupational exposure to magnetic fields experiment rat single cage fixing device Download PDFInfo
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- CN106725977A CN106725977A CN201611240094.5A CN201611240094A CN106725977A CN 106725977 A CN106725977 A CN 106725977A CN 201611240094 A CN201611240094 A CN 201611240094A CN 106725977 A CN106725977 A CN 106725977A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
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Abstract
The invention discloses a kind of Occupational exposure to magnetic fields experiment rat single cage fixing device, including：Mouse cage frame, defines the cavity of cuboid in mouse cage frame, the both ends open of the length direction of cavity, mouse cage sets up the installation through-hole for having and being connected with cavity；Two gate baffle plates, two gate baffle plates are movably connected with the two ends of mouse cage frame to be opened and closed two open ends of cavity, and multiple air-vents of connection cavity and the external world are respectively equipped with gate baffle plate and mouse cage frame；Controllable register, controllable register is located in installation through-hole in which can pull and push, and cavity can along its length be divided into two sub- chambers when controllable register is located in installation through-hole.Mouse cage fixing device according to embodiments of the present invention can not be limited by miniaturized radiation source, narrow uniform field, can improve dose of radiation precision to 25.45%, and be easy to the loading of animal.Using apparatus of the present invention, the biological effect research that high-frequency short waves radiation causes rat brain damage has been successfully applied to.
Description
Technical field
The present invention relates to experiment equipment technical field, consolidated with rat single cage more particularly, to a kind of experiment of Occupational exposure to magnetic fields
Determine device.
Background technology
With the development of science and technology, the high frequency such as shortwave or microwave electromagnetic equipment is in necks such as information, communication, industry and military affairs
The application in domain is increasingly extensive, and the mankind are more and more among electromagnetic radiation environment.Research it has been shown that electromagnetic field it is short
Phase exposes repeatedly, can cause nerve, reproduction and the dysfunction such as immune, especially has harm to learning and memory function,
Cause the extensive concern of domestic and foreign scholars.
In some correlation techniques, the intensity of electromagnetic radiation field of experimental study selection, open-assembly time, study subject and spoke
Penetrate simulation source different, the Board Lot that Research in electromagnetic radiation can be carried out in addition is limited, cause result of study to be still lack of consistency knot
By.Occupational exposure to magnetic fields experiment rat fixing device of the prior art is generally to meet large radiation simulation source and design, its build
It is larger, it is impossible to ensure the miniaturization of radiomimesis source, radiation uniform field scope it is narrower etc. under the conditions of zoopery effectively carry out；
And existing rat fixing device uses upper opening type, push-and-pull overhead gage during sealing or so often occurs and crushes rat-tail etc.
Problem.Above many factors have impact on being widely popularized and being smoothed out for electromagnetic radiation biological effect research.
The content of the invention
It is contemplated that at least solving one of technical problem in correlation technique to a certain extent or providing at one kind
Useful business selection.Therefore, it is an object of the present invention to propose that a kind of Occupational exposure to magnetic fields experiment fixes dress with rat single cage
Put, the mouse cage fixing device can improve the precision of experiment and be easy to animal to load, fixing device successful Application
In high-frequency short waves radiation causes the biological effect research of Brain Function of Rats and structural damage.
Occupational exposure to magnetic fields experiment rat single cage fixing device according to embodiments of the present invention, including：Mouse cage frame, the mouse cage
The cavity of cuboid is defined in frame, the both ends open of the length direction of the cavity, the mouse cage erection has and the sky
The installation through-hole of chamber connection；Two gate baffle plates, two two ends for gating baffle plate movably with the mouse cage frame are connected
To be opened and closed two open ends of the cavity, be respectively equipped with the gate baffle plate and the mouse cage frame connection cavity with it is outer
Multiple air-vents on boundary；Controllable register, the controllable register is located in the installation through-hole in which can pull and push, and the cavity is described
Controllable register can along its length be divided into two sub- chambers when being located in the installation through-hole.
Mouse cage fixing device according to embodiments of the present invention can flexibly realize the size adjustment of rat receiving space, regulation
Flexibly, study subject whole body can be made to receive homogeneous radiation in being located at homogeneous radiation, the precision of experiment is improve, using this
Mouse cage fixing device carries out Occupational exposure to magnetic fields experiment can not the miniaturization of raying simulation source, the radiation condition limitation such as uniform field is narrow；
Meanwhile, the device more meets experimental animal and gets into the cave nature, be easy to animal load with it is fixed, it is more convenient to operate.The fixing device is
It is successfully applied in the biological effect research of high-frequency short waves radiation cause Brain Function of Rats and structural damage, and finds 10~30mW/
cm2High-frequency short waves radiation can cause learning and memory in rats ability to decline, brain Electricity Functional is disorderly, hippocampal tissue structural damage.
In addition, Occupational exposure to magnetic fields experiment according to the above embodiment of the present invention can also have such as with rat single cage fixing device
Lower additional technical characteristic：
Some embodiments of the invention, two gate baffle plates and the controllable register are relative to the mouse cage frame
Vertically may move, be respectively equipped with the middle part of the lower end of the controllable register and the gate baffle plate and connected with the cavity
Avoidance breach, the avoidance breach be formed as the length of side be 1 ± 0.5cm square opening.
Some embodiments of the invention, the mouse cage frame is respectively arranged at two ends with slot, two gate baffle plates
It is located at can pulling and pushing in the corresponding slot.
Some embodiments of the invention, each air-vent is formed as the circular port of a diameter of 1.5 ± 0.5cm.
The water that some embodiments of the invention, the controllable register and the gate baffle plate adjacent thereto are spaced
Flat distance is 5 ± 1cm, and the controllable register is provided with air-vent described at least one.
Some embodiments of the invention, the mouse cage frame includes：Upper level board and lower horizontal board, the upper level board
And vertically spaced apart setting horizontally extending with lower horizontal board difference；First vertical plate and second vertical
Plate, first vertical plate and second vertical plate vertically extend respectively and horizontally spaced opening up is put, institute
The first vertical plate, the upper level board, second vertical plate and the lower horizontal board is stated to be vertically connected successively and limit institute
Cavity is stated, multiple air-vents are respectively equipped with the upper level board, first vertical plate and second vertical plate.
Some embodiments of the invention, the lower horizontal board be thickness for 10 ± 0.2mm acrylic board, it is described on
It is 5 that level board, first vertical plate, second vertical plate, the gate baffle plate and the controllable register are respectively thickness
The acrylic board of ± 0.2mm.
Some embodiments of the invention, the length of the mouse cage frame is 20 ± 1cm, and width is 7 ± 1cm, is highly 8
±1cm。
Thus, tested by the Occupational exposure to magnetic fields using the above embodiment of the present invention and use rat single cage fixing device, most I
It is 140cm to meet radiation uniform field size2Radiation source requirement, fixed can be improved about compared to experiment precision with unguyed
25.45%, the biological effect research that shortwave radiation causes rat brain damage has been successfully applied to, find 10~30mW/cm2High frequency is short
Wave radiation can cause learning and memory in rats ability to decline；Brain Electricity Functional is disorderly, is mainly shown as the reduction of fast wave β power, slow wave δ and
θ power is raised；Hippocampal tissue structural damage.
Additional aspect of the invention and advantage will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by practice of the invention.
Brief description of the drawings
Fig. 1 is the structural representation of Occupational exposure to magnetic fields experiment rat single cage fixing device according to embodiments of the present invention, its
In, mouse cage fixing device is closed；
Fig. 2 is the structural representation of Occupational exposure to magnetic fields experiment rat single cage fixing device according to embodiments of the present invention, its
In, mouse cage fixing device is in open mode；
Fig. 3 is planar structure and the rat representative moving position top view in radiation field of shortwave radiation；
Fig. 4 is the rat radiation intensity analogue simulation figure that diverse location receives in radiation field, wherein：
4 (a) is Electric Field Distribution sectional drawing of the rat in radiation field, and 4 (b) is radiation field intensity dosage simulation result；
Fig. 5 is Rat hippocampus structure according to embodiments of the present invention by 0,5,10 and 30mW/cm2Shortwave radiation
The Rat hippocampus structure chart of the 1st day afterwards；
Fig. 6 is Rat hippocampus structure according to embodiments of the present invention by 0,5,10 and 30mW/cm2Shortwave radiation
The Rat hippocampus structure chart of the 7th day afterwards；
Fig. 7 is Rat hippocampus structure according to embodiments of the present invention by 0,5,10 and 30mW/cm2Shortwave radiation
The Rat hippocampus structure chart of the 14th day afterwards；
Fig. 8 is Rat hippocampus structure according to embodiments of the present invention by 0,5,10 and 30mW/cm2Shortwave radiation
The Rat hippocampus structure chart of the 28th day afterwards；
Wherein, in Fig. 5 to Fig. 8, the lower left corner is marked numeral 0,5,10 and 30 and represents Normal group, 5mW/cm respectively2
Group, 10mW/cm2Group and 30mW/cm2Group, arrow represents the nerve cell of pyknosis dye deeply.
Reference：
Mouse cage fixing device 100；
Mouse cage frame 10；Upper level board 11；Lower horizontal board 12；First vertical plate 13；Second vertical plate 14；
Gate baffle plate 20；
Controllable register 30；
Air-vent 101；Installation through-hole 102；Avoid breach 103.
Specific embodiment
Embodiments of the invention are described below in detail, the example of the embodiment is shown in the drawings.Below with reference to
The embodiment of Description of Drawings is exemplary, it is intended to for explaining the present invention, and be not considered as limiting the invention, ability
The those of ordinary skill in domain can be changed to above-described embodiment, change, replacing and modification within the scope of the invention.
Describe Occupational exposure to magnetic fields experiment rat single cage fixing device according to embodiments of the present invention in detail below in conjunction with the accompanying drawings
100。
Shown referring to Figures 1 and 2, according to embodiments of the present invention Occupational exposure to magnetic fields experiment rat single cage fixing device 100
May include mouse cage frame 10, two gates baffle plate 20 and controllable registers 30.
The cavity of cuboid is defined in mouse cage frame 10, when mouse cage fixing device 100 is normally placed, cavity can be along water
Square to extending and use as rat receiving space.The both ends open of the length direction in mouse chamber, i.e., along the length direction of cavity
On, the two ends of cavity are opened wide form two open ends respectively.Two gate baffle plate 20 movably with the two ends phase of mouse cage frame 10
Even, to open and close two open ends in mouse chamber.It is specific when in use, one of them in two open ends can be as entering
Mouth end, another can be as the port of export, to facilitate operation.
Gate baffle plate 20 and mouse cage frame 10 are provided with air-vent 101, and air-vent 101 can connect cavity and the external world, make mouse
Cage fixing device 100 can realize that inner air and outer air circulates, it is to avoid sultry, reduce non-empirical factor interference, be conducive to the essence of experiment
Parasexuality.In the present invention, do not do specifically limited for the set location of air-vent 101, except in gate baffle plate 20 and mouse cage frame
Set outside air-vent 101 simultaneously on 10, air-vent 101 only can also be set on mouse cage frame 10.In addition, ventilative in order to improve
Effect, can also set air-vent 101 on controllable register 30.There is no particular/special requirement for the quantity of air-vent 101, for example,
Can be for five, ten or more etc., can flexibly be set according to pore size and ventilative intensity requirement etc..
Mouse cage frame 10 is provided with installation through-hole 102, and installation through-hole 102 is connected with mouse chamber, and controllable register 30 sets in which can pull and push
In installation through-hole 102, mouse chamber is located in installation through-hole 102 in controllable register 30 and is divided into two sub- chambers along its length.
That is, controllable register 30 may be inserted into installation through-hole 102, it is also possible to extracted out in from installation through-hole 102, work as controllable register
30 are not inserted into when in installation through-hole 102, generally one chamber of cavity, when controllable register 30 is inserted into installation through-hole 102, mouse
Chamber can be conditioned baffle plate 30 and be separated into two sub- chambers along the length direction of itself.
Thus, it is possible to pass through controllable register 30, the size adjustment of rat receiving space is realized.It is also possible to by multiple
Mouse cage fixing device 100 is stitched together, and by adjusting the opening and closing of gate baffle plate 20, realizes the big ditty of rat receiving space
Section.I.e. in the present invention, the big ditty of rat receiving space is realized in cooperation that can be by gating baffle plate 20 and controllable register 30
Section.
Mouse cage fixing device 100 according to embodiments of the present invention, by set both ends open mouse cage frame 10 and can be with
The gate baffle plate 20 and controllable register 30 being movably connected therewith, can flexibly realize the size adjustment of rat receiving space, regulation
Flexibly, when specifically used, can be used using free-standing single cage structure, also can be according to uniform field range size by multiple single cages
Structural union is used, so that study subject whole body receives homogeneous radiation in being located at homogeneous radiation, realizes miniaturization radiation
The homogeneous radiation of the single rat in source, improves the precision of experiment.Meanwhile, the both ends open formula that mouse cage fixing device 100 has
Hole type structure, more meet experimental animal and get into the cave nature, easily facilitate animal load with it is fixed, it is more convenient to operate.
The device has been successfully applied in the biological effect research of high-frequency short waves radiation cause Brain Function of Rats and structural damage,
It is 140cm that most I meets radiation uniform field size2Radiation source requirement, it is more smart than dose of radiation with the unused device fixing phase
Accuracy can improve about 25.45%, find 10~30mW/cm2High-frequency short waves radiation can cause learning and memory in rats ability to decline；Brain
Electricity Functional is disorderly, is mainly shown as the reduction of fast wave β power, and slow wave δ and θ power are raised；Hippocampal tissue structural damage.
As depicted in figs. 1 and 2, two gate baffle plates 20 can be moved vertically relative to mouse cage frame 10, controllable register
30 also can vertically move relative to mouse cage frame 10.Thus, it is possible to pass through to move up and down gate baffle plate 20 and controllable register
30 opening and closing for realizing rat receiving space and size adjustment, more convenient operation.
Rat fixing device in correlation technique uses upper opening type, and push-and-pull overhead gage easily exists and crushes during sealing or so
The problems such as rat-tail, animal places inconvenience, have impact on being widely popularized and being smoothed out for electromagnetic radiation biological effect research.Favorably
Ground, in the present invention, is provided with avoidance breach 103 in the middle part of the lower end for gating baffle plate 20, avoid breach 103 and connected with cavity.When
During to placing rat in cavity, avoid breach 103 effectively can avoid to rat-tail, prevent from disturbing door because rat-tail is long
The fixation of baffle plate 20 is controlled, while avoid the gate baffle plate 20 when rat-tail is also introduced into in mouse chamber from closing and clip to rat-tail, effectively
Solve the problems, such as to crush rat-tail in the presence of correlation technique, further ensure experiment be smoothed out and rat is put into more
It is smooth convenient.
Alternatively, the middle part of the lower end of controllable register 30 can also set avoidance breach 103, with when space size is adjusted
Rat-tail is avoided, after preferably ensureing that rat is pierced in corresponding sub- chamber, controllable register 30 can be inserted smoothly, further
Prevent from crushing rat-tail, be more beneficial for successfully launching experiment.
Size and dimension for avoiding breach 103 does not do specifically limited, for example, avoiding breach 103 can be formed as half
Circular port, polygonal hole or irregular shape hole etc..Preferably, avoid breach 103 and be formed as square opening, the side of square opening
A length of 1 ± 0.5cm.The size is applicable to most of experiment, and applicability is wide and avoidance effect is good.
Some embodiments of the invention, mouse cage frame 10 is respectively arranged at two ends with slot, and two gate baffle plates 20 can take out
It is located at slottingly in corresponding slot.Thus, slot can carry out positioning and spacing to gate baffle plate 20, install gate baffle plate 20
It is accurate and firm, it is particularly possible to be fixed on the length direction in mouse chamber, it is to avoid gate baffle plate 20 is anticipated by the shock of rat
Outer opening, it is fixed and be difficult to shake off.Preferably, two gate baffle plate 20 be located at movably in a vertical direction it is corresponding
In slot, in operation, can straight up extract realization out and open, insertion straight down is realized closing, and switching manipulation is more convenient simultaneously
And it is easy to manufacture.
To improve the fixed effect of controllable register 30, slot can also be set in the relative interior sidewall surface in mouse chamber, make tune
Section baffle plate 30 can be not only fixed by installation through-hole 102, and the both sides of controllable register 30 can be entered by slot
Row is fixed, and controllable register 30 is connected more reliable with mouse cage frame 10.
Alternatively, the horizontal range that controllable register 30 and gate baffle plate 20 adjacent thereto are spaced is 5 ± 1cm.Change speech
It, the horizontal range that gate baffle plate 20 and the controllable register 30 neighbouring with controllable register 30 is spaced is 5 ± 1cm, i.e. regulation gear
Plate 30 is located substantially at away from a position of the 5 ± 1cm of horizontal range of open end, and regulating effect is good, can meet different size build
Rat is fixed and requires.
Some embodiments of the invention, air-vent 101 is formed as the circular port of a diameter of 1.5 ± 0.5cm.By
This, can preferably ensure that inner air and outer air circulates, and the profile of mouse cage fixing device 100 is more attractive in appearance.As depicted in figs. 1 and 2,
Multiple air-vents 101 for being spaced apart and setting are respectively equipped with mouse cage frame 10, gate baffle plate 20 and controllable register 30, mouse cage fixes dress
The air permeable effect for putting 100 is excellent, while the state from the rat in the viewing test of outside can also be facilitated, it is tentative good.
As depicted in figs. 1 and 2, mouse cage frame 10 may include level board 11, lower horizontal board 12, the first vertical plate 13 and second
Vertical plate 14.Upper level board 11 and lower horizontal board 12 distinguish horizontally extending and vertically setting spaced apart.The
One vertical plate 13 and the second vertical plate 14 vertically extend respectively and horizontally spaced opening up is put.First vertical plate
13rd, upper level board 11, the second vertical plate 14 and lower horizontal board 12 are vertically connected and limit cavity successively.Upper level board 11,
Air-vent 101 is respectively equipped with one vertical plate 13 and the second vertical plate 14.Thus, not only facilitate manufacture but also air permeable effect be good,
In addition, lower horizontal board 12 is tight non-porous, experimental animal stool and urine pollution instrument and equipment in radiative process is received can be prevented.
Some specific examples of the invention, lower horizontal board 12, upper level board 11, the first vertical plate 13, second are vertical
Plate 14, gate baffle plate 20 and controllable register 30 are acrylic board.Acrylic board is poly (methyl methacrylate) plate, and this kind of material is nonpoisonous and tasteless
And mechanical strength is higher, it is ensured that animal health simultaneously prevents it from biting, and the material do not interfere with electromagnetic wave propagation with
Distribution, and then reduce experiment interference factor.Further, the thickness of lower horizontal board 12 is 10 ± 0.2mm, and the thickness of remaining plate is
5±0.2mm.Thus, the bottom surface of mouse cage frame 10 is thicker, and its lap is slightly light, and the slightly light structure design in this thick and heavy upper end in bottom can
Increase the stability of cage, effectively prevent from toppling over.
Alternatively, the length of mouse cage frame 10 can be 20 ± 1cm, and width can be 7 ± 1cm, highly can be 8 ± 1cm.Thus,
The requirement for limiting rat voluntary activity can be met, be unlikely to too to fetter again.
A specific example of mouse cage fixing device 100 according to embodiments of the present invention is shown in Fig. 1 and Fig. 2.Such as Fig. 1
With shown in Fig. 2, mouse cage fixing device 100 includes mouse cage frame 10, two gates baffle plate 20 and controllable registers 30, and mouse cage frame 10 is formed
It is porous cage, by inter-adhesive cavity that a centre is cuboid and the both ends open of surrounding of four rectangular flat boards
Integral structure, wherein one in four flat boards is without hole plate and thickness is relatively thick, as mouse cage fixing device 100
Bottom surface, remaining three flat board is porous flat plate and thickness is relatively thin, as mouse cage fixing device 100 two sides and
Top surface.Two gate baffle plates 20 and controllable register 30 are located on mouse cage frame 10 in which can vertically pull and push.Flat board, gate baffle plate
20 and controllable register 30 on air-vent 101 for a diameter of 1.5cm circular port.
The correlation test carried out using above-mentioned mouse cage fixing device 100 is described in detail below.
(A) fixed position of the rat in radiation field：Radiation field center section is the rectangular region of rule, and two ends are not for
Regular triangle region.If rat is not fixed free activity in radiation field, there is position 1~5 etc. representative zone of action
(such as Fig. 3 shows).Draw radiation field intensity distribution map that rat is subject in diverse location (such as Fig. 4 institutes using computer simulation
Show).By can be calculated：Rat is fixed at position 1 with mouse cage fixing device, is fixed with the unused device and in place
Put free activity at 1~5 to compare, the accuracy of its suffered dose of radiation improves 25.45%.Therefore, in subsequent experimental
Rat is fixed at position 1 using the device is radiated.
(B) shortwave radiation is carried out to rat：By two grades of male Wistar rat (armies that 100 body weight are 200 ± 20 grams
Thing Academy of Medical Sciences Experimental Animal Center is provided), it is randomly divided into four groups：Normal group, 5mW/cm2Group, 10mW/cm2Group and
30mW/cm2Group, every group 25.Using the shortwave radiation source of Ministry of Industry and Information's TRE telecommunications research establishment, average power density is respectively 5,10 and
30mW/cm2Shortwave to rat radiate, radiated time 6min.Rat is placed in mouse cage fixing device and fixation postures.Control
Group carries out false radiation, that is, close radiation source, and other conditions are the same.
(C) learning and memory in rats ability detection：Learning and memory in rats ability is detected using Morris water mazes test system
Change.The system is made up of stainless steel round pool (120 centimetres of diameter, high 50 centimetres), video camera, computer for analysis software.
Pond is divided into 4 quadrants by 20 centimetres of control water depth when using, the degrees centigrade of water temperature 23, each quadrant pool wall centre position
It is place of entry.Place 18 centimetres of diameter, 18 centimetres high dark platforms in optionally wherein 1 quadrant centre position.Pond top peace
Video camera is put, is connected with software systems, it is continuous to record rats'swimming track to be analyzed.
Each group rat acclimatization training 2 days before shortwave radiation, were positioned in 1~4 day, 7 days, 14 days and 28 days after radiation
Navigation experiment.Rat is put into water from 4 place of entry respectively towards pool wall, its time that platform was found in 60 seconds is recorded
(escape latency), the average value using 4 quadrants is average escape latency as observation index.If not finding platform in 60 seconds
Then it is designated as 60 seconds, proceeds next quadrant experiment.The results are shown in Table 1.
Influence (second) of the shortwave radiation of table 1 to rat average escape latency
Remarks：Compared with control group, * P<0.05, * * P<0.01
From result in table 1, as experiment number increases, average escape latency is in certain period of time for 4 groups of rats
In trend is gradually shortened.Compared with control group, 5mW/cm2Group rat average escape latency substantially changes in nothing of each time point
Become, 10 and 30mW/cm2Group rat was obviously prolonged (P at 1~7 day<0.05), to after 14 days without significant difference.It can thus be appreciated that 10 Hes
30mW/cm2Shortwave radiation can cause the damage of rat Spatial memory ability.
(D) detection of rat electroencephalogram：After irradiation 1 day, 7 days, 14 days and 28 days respectively, 1% penta bars are implemented to rat
Than appropriate sodium intraperitoneal anesthesia (0.5 milliliter/100 grams), cause rat in light anesthesia state, using leading MP-150 more physiology record and point
Analysis system record analysis rat electroencephalogram.Preserved skin does not place an electrode after rat crown middle section, and left ear-lobe places reference
Electrode, needle electrode is fixed on subcutaneous, continuous record 5 minutes, and 4 kinds of changed power feelings of brain wave α, β, δ and θ are analyzed respectively
Condition.The results are shown in Table 2 to table 5.
Influence (μ V of the shortwave radiation of table 2 to the power of rat brain wave α2)
Influence (μ V of the shortwave radiation of table 3 to the power of rat brain wave β2)
Influence (μ V of the shortwave radiation of table 4 to the power of rat brain wave δ2)
Influence (μ V of the shortwave radiation of table 5 to the power of rat brain wave θ2)
Remarks：Compared with control group, * P<0.05, * * P<0.01
From result in table, 4 groups of α wave powers of rat are in equal no significant difference of each time point；β wave powers were at 1 day
Each radiation group is reduced, and shows the lower change of bigger its power of dose of radiation, with extension each group difference of each time point gradually
Reduce, to each group no significant difference at 28 days；θ and δ wave powers are in 5mW/cm2Group is without significantly rising, 10 and 30mW/cm2Group is obvious
Raise, with dosage positive correlation, normal level is gradually brought to extension of each time point radiation group.Illustrate the shortwave of doses
Radiation can cause rat brain Electricity Functional disorderly, be mainly shown as 10 and 30mW/cm2Shortwave radiation can cause the reduction of fast wave β power, slowly
Ripple δ and θ power are raised.
(E) rat hippocampal Observation of Histological Structure：It is right by 4 groups of rats respectively at 1 day, 7 days, 14 days and 28 days after radiation
After rat implements 1% yellow Jackets intraperitoneal anesthesia (1.5 milliliters/100 grams), sacrificed by decapitation separates cerebral hippocampal tissue, away from forebrain 6
Make coronal section (hippocampus position) at millimeter, take after the buffered formalin of latter half brain tissue 10% fixes 1 week, it is conventional de-
Water, transparent, waxdip, embedding, make 3 μ m-thick paraffin sections, HE dyeing, om observation and Taking Pictures recording, as a result such as Fig. 5 to Fig. 8
It is shown.
Om observation, control rats hippocampal formation dentate granule cell and CA3 areas centrum are dyeed from brain tissue
Cell arrangement is neat, and neuron morphology is full, and cytoplasm is uniform.Compared with control group, each radiation group nothing significantly changes at 1 day
Become；5mW/cm at 7 days2There is slight pyknosis and contaminates deeply in a small amount of neuron of group rat hippocampus, 10mW/cm2The neuron pyknosis depth of group
Dye change increases, 30mW/cm2There is the neuron of a large amount of pyknosis dye deeply in group；Each radiation group neuron pyknosis is caught an illness deeply at 14 days
Change shows the trend of mitigation；To after radiating the 28th day, each radiation group neuron recovers to normal level substantially.Show certain agent
The shortwave radiation of amount can cause brain tissue structural damage.
Other of mouse cage fixing device 100 according to embodiments of the present invention are constituted and operate the common skill for this area
It is knowable for art personnel, is not described in detail herein.
In the description of the invention, it is to be understood that term " length ", " thickness ", " on ", D score, "front", "rear",
The orientation or position relationship of the instruction such as "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", " outward " are based on accompanying drawing institute
The orientation or position relationship for showing, are for only for ease of the description present invention and simplify description, rather than the dress for indicating or implying meaning
Put or element must have specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to limit of the invention
System.
Additionally, term " first ", " second " are only used for describing purpose, and it is not intended that indicating or implying relative importance
Or the implicit quantity for indicating indicated technical characteristic.Thus, define " first ", the feature of " second " can express or
Implicitly include at least one this feature.In the description of the invention, " multiple " is meant that at least two, such as two, three
It is individual etc., unless otherwise clearly defined and limited；Fisrt feature second feature " on " can be that the first and second features are direct
Contact, or the first and second features pass through intermediary mediate contact, unless otherwise expressly limited specifically.
In the present invention, unless otherwise clearly defined and limited, term " installation ", " connected ", " connection ", " fixation " etc.
Term should be interpreted broadly, for example, it may be fixedly connected, or be detachably connected, or integrally；Can be that machinery connects
Connect, or electrically connect or can communicate each other；Can be joined directly together, it is also possible to be indirectly connected to by intermediary, can be with
Be two element internals connection or two interaction relationships of element, unless otherwise clearly restriction.For this area
For those of ordinary skill, above-mentioned term concrete meaning in the present invention can be as the case may be understood.
In the description of this specification, the description of reference term " embodiment " or " specific example " etc. means to combine the implementation
Example or the specific features of example description, structure, material or feature are contained at least one embodiment of the invention or example.
In this manual, the schematic representation to above-mentioned term is necessarily directed to identical embodiment or example.And, description
Specific features, structure, material or feature can in an appropriate manner combine in any one or more embodiments or example.
Additionally, in the case of not conflicting, those skilled in the art can by the different embodiments described in this specification or
The feature of example and different embodiments or example is combined and combines.
Claims (8)
1. a kind of Occupational exposure to magnetic fields is tested and uses rat single cage fixing device, it is characterised in that including：
Mouse cage frame, defines the cavity of cuboid in the mouse cage frame, the both ends open of the length direction of the cavity is described
Mouse cage sets up the installation through-hole for having and being connected with the cavity；
Two gate baffle plates, two gate baffle plates movably are connected to be opened and closed the cavity with the two ends of the mouse cage frame
Two open ends, the connection cavity is respectively equipped with the gate baffle plate and the mouse cage frame and is breathed freely with the multiple in the external world
Hole；
Controllable register, the controllable register is located in the installation through-hole in which can pull and push, and the cavity is in controllable register position
Two sub- chambers can be along its length divided into when in the installation through-hole.
2. Occupational exposure to magnetic fields according to claim 1 is tested and uses rat single cage fixing device, it is characterised in that two doors
Control baffle plate and the controllable register vertically may move relative to the mouse cage frame, and the controllable register and the gate keep off
Be respectively equipped with the avoidance breach connected with the cavity in the middle part of the lower end of plate, the avoidance breach be formed as the length of side for 1 ±
The square opening of 0.5cm.
3. Occupational exposure to magnetic fields according to claim 1 is tested and uses rat single cage fixing device, it is characterised in that the mouse cage frame
Be respectively arranged at two ends with slot, two gate baffle plates are located in the corresponding slot in which can pull and push.
4. Occupational exposure to magnetic fields according to claim 1 is tested and uses rat single cage fixing device, it is characterised in that each is described
Stomatal limiting value is the circular port of a diameter of 1.5 ± 0.5cm.
5. Occupational exposure to magnetic fields according to claim 1 is tested and uses rat single cage fixing device, it is characterised in that the regulation gear
The horizontal range that plate and the gate baffle plate adjacent thereto are spaced is 5 ± 1cm, and the controllable register is provided with least one
The air-vent.
6. the Occupational exposure to magnetic fields according to any one of claim 1-5 is tested and uses rat single cage fixing device, it is characterised in that
The mouse cage frame includes：
Upper level board and lower horizontal board, the upper level board and lower horizontal board difference are horizontally extending and along vertical side
To setting spaced apart；
First vertical plate and the second vertical plate, first vertical plate and second vertical plate vertically extend respectively and
Horizontally spaced opening up is put, first vertical plate, the upper level board, second vertical plate and the lower horizontal board
Be vertically connected and limit the cavity successively, the upper level board, first vertical plate and second vertical plate it is upper
It is respectively equipped with multiple air-vents.
7. Occupational exposure to magnetic fields according to claim 8 is tested and uses rat single cage fixing device, it is characterised in that the lower level
Plate is that thickness is the acrylic board of 10 ± 0.2mm, the upper level board, first vertical plate, second vertical plate, described
Gate baffle plate and the controllable register are respectively the acrylic board that thickness is 5 ± 0.2mm.
8. Occupational exposure to magnetic fields according to claim 8 is tested and uses rat single cage fixing device, it is characterised in that the mouse cage frame
Length be 20 ± 1cm, width be 7 ± 1cm, be highly 8 ± 1cm.
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| CN201611240094.5A CN106725977B (en) | 2016-12-28 | 2016-12-28 | Occupational exposure to magnetic fields experiment rat single cage fixing device |
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| CN201611240094.5A CN106725977B (en) | 2016-12-28 | 2016-12-28 | Occupational exposure to magnetic fields experiment rat single cage fixing device |
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| CN106725977B CN106725977B (en) | 2018-10-09 |
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| CN109363795A (en) * | 2018-09-29 | 2019-02-22 | 东南大学 | Microwave irradiation device and method suitable for open field |
| CN110074891A (en) * | 2018-01-25 | 2019-08-02 | 中国辐射防护研究院 | Irradiation test rat fixing device |
| CN111418518A (en) * | 2020-05-19 | 2020-07-17 | 河南省人民医院 | Water labyrinth device in experimental research of cerebral ischemic rat cognitive dysfunction |
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| CN112773549A (en) * | 2019-11-07 | 2021-05-11 | 中国人民解放军军事科学院军事医学研究院 | Positionable rodent immobilization device for electromagnetic radiation exposure |
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