CN106725977B - Occupational exposure to magnetic fields experiment rat single cage fixing device - Google Patents
Occupational exposure to magnetic fields experiment rat single cage fixing device Download PDFInfo
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- CN106725977B CN106725977B CN201611240094.5A CN201611240094A CN106725977B CN 106725977 B CN106725977 B CN 106725977B CN 201611240094 A CN201611240094 A CN 201611240094A CN 106725977 B CN106725977 B CN 106725977B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
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Abstract
The invention discloses a kind of Occupational exposure to magnetic fields experiment rat single cage fixing devices, including：Mouse cage frame, the cavity of cuboid, the both ends open of the length direction of cavity are defined in mouse cage frame, and mouse cage is provided with the installation through-hole being connected to cavity；Two gate baffles, two gate baffles are movably connected to be opened and closed two open ends of cavity with the both ends of mouse cage frame, gate and be respectively equipped with connection cavity and extraneous multiple air holes on baffle and mouse cage frame；Controllable register, controllable register are located in installation through-hole in which can pull and push, and cavity can be divided into two sub- chambers when controllable register is located in installation through-hole along its length.Mouse cage fixing device according to the ... of the embodiment of the present invention can not be limited by miniaturized radiation source, narrow uniform field, and dose of radiation precision can be improved to 25.45%, and convenient for the loading of animal.Using apparatus of the present invention, it is successfully applied to the biological effect research that high-frequency short waves radiation causes rat brain damage.
Description
Technical field
The present invention relates to experiment equipment technical fields, solid with rat single cage more particularly, to a kind of experiment of Occupational exposure to magnetic fields
Determine device.
Background technology
With the development of science and technology, the high frequencies such as shortwave or microwave electromagnetic equipment is in necks such as information, communication, industry and military affairs
The application in domain is increasingly extensive, and the mankind are exposed among electromagnetic radiation environment more and more.Research it has been shown that electromagnetic field it is short
Phase exposes repeatedly, can cause nerve, reproduction and the dysfunctions such as immune, especially harmful to learning and memory function,
Cause the extensive concern of domestic and foreign scholars.
Some in the related technology, experimental study selection electromagnetic radiation field intensity, exposure duration, study subject and spoke
Simulation source difference is penetrated, the Board Lot that can carry out Research in electromagnetic radiation in addition is limited, and result of study is caused still to be lack of consistency knot
By.Occupational exposure to magnetic fields experiment rat fixing device in the prior art is mostly to meet large radiation simulation source and design, build
It is larger, it can not ensure effective progress of zoopery under the conditions of the miniaturization of radiomimesis source, radiation uniform field range are narrower etc.；
And existing rat fixing device is all made of upper opening type, and push-and-pull overhead gage when sealing or so often will appear and crush rat-tail etc.
Problem.The above many factors affect being widely popularized and being smoothed out for electromagnetic radiation biological effect research.
Invention content
The present invention is directed to solve at least some of the technical problems in related technologies or at least provide one kind
Useful business selection.For this purpose, an object of the present invention is to provide a kind of experiments of Occupational exposure to magnetic fields dress is fixed with rat single cage
It sets, the mouse cage fixing device can improve the precision of experiment and is packed into convenient for animal, which has been applied successfully
It is radiated in high-frequency short waves in the biological effect research for causing Brain Function of Rats and structural damage.
Occupational exposure to magnetic fields experiment rat single cage fixing device according to the ... of the embodiment of the present invention, including：Mouse cage frame, the mouse cage
The cavity of cuboid, the both ends open of the length direction of the cavity are defined in frame, the mouse cage is provided with and the sky
The installation through-hole of chamber connection；Two gate baffles, two gate baffles are movably connected with the both ends of the mouse cage frame
To be opened and closed two open ends of the cavity, be respectively equipped on the gate baffle and the mouse cage frame connection cavity with it is outer
Multiple air holes on boundary；Controllable register, the controllable register are located in the installation through-hole in which can pull and push, and the cavity is described
Controllable register can be divided into two sub- chambers along its length when being located in the installation through-hole.
Mouse cage fixing device according to the ... of the embodiment of the present invention can flexibly realize the big minor adjustment of rat accommodation space, adjust
Flexibly, study subject whole body can be made to be located in homogeneous radiation field and receive homogeneous radiation, the precision of experiment is improved, utilize this
Mouse cage fixing device carries out Occupational exposure to magnetic fields experiment can not the miniaturization of raying simulation source, the radiation conditions limitation such as uniform field is narrow；
Meanwhile the device more meets experimental animal and gets into the cave nature, is packed into convenient for animal and fixed, operation is more convenient.The fixing device is
It is successfully applied in the biological effect research that high-frequency short waves radiation causes Brain Function of Rats and structural damage, and finds 10~30mW/
cm2High-frequency short waves radiation can cause learning and memory in rats ability to decline, brain Electricity Functional is disorderly, hippocampal tissue structural damage.
In addition, Occupational exposure to magnetic fields experiment according to the above embodiment of the present invention can also have such as with rat single cage fixing device
Under additional technical characteristic：
According to some embodiments of the present invention, two gate baffles and the controllable register are relative to the mouse cage frame
It vertically moves, is respectively equipped in the middle part of the lower end of the controllable register and the gate baffle and is connected to the cavity
Evacuation notch, it is described evacuation notch be formed as the length of side be 1 ± 0.5cm square hole.
According to some embodiments of the present invention, the both ends of the mouse cage frame are respectively equipped with slot, two gate baffles
It is located at can pulling and pushing in the corresponding slot.
According to some embodiments of the present invention, each air hole is formed as the round hole of a diameter of 1.5 ± 0.5cm.
According to some embodiments of the present invention, the water that the controllable register and the gate baffle adjacent thereto are spaced
Flat distance is 5 ± 1cm, and the controllable register is equipped at least one air hole.
According to some embodiments of the present invention, the mouse cage frame includes：Upper level board and lower horizontal board, the upper level board
And vertically spaced apart setting horizontally extending with lower horizontal board difference；First vertical plate and second is vertically
Plate, first vertical plate and second vertical plate vertically extend respectively and horizontally spaced open up is set, institute
The first vertical plate, the upper level board, second vertical plate and the lower horizontal board is stated to be vertically connected successively and limit institute
Cavity is stated, multiple air holes are respectively equipped on the upper level board, first vertical plate and second vertical plate.
According to some embodiments of the present invention, the lower horizontal board is the acrylic board that thickness is 10 ± 0.2mm, it is described on
Level board, first vertical plate, second vertical plate, the gate baffle and the controllable register are respectively that thickness is 5
The acrylic board of ± 0.2mm.
According to some embodiments of the present invention, the length of the mouse cage frame is 20 ± 1cm, and width is 7 ± 1cm, is highly 8
±1cm。
As a result, by using the Occupational exposure to magnetic fields of the above embodiment of the present invention experiment rat single cage fixing device, minimum can
It is 140cm to meet radiation uniform field size2Radiation source requirement, fixed can be improved about compared to experiment precision with unguyed
25.45%, it has been successfully applied to the biological effect research that shortwave radiation causes rat brain damage, has found 10~30mW/cm2High frequency is short
Wave radiation can cause learning and memory in rats ability to decline；Brain Electricity Functional is disorderly, is mainly shown as the reduction of fast wave β power, slow wave δ and
θ power increases；Hippocampal tissue structural damage.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obviously, or practice through the invention is recognized.
Description of the drawings
Fig. 1 is the structural schematic diagram of Occupational exposure to magnetic fields experiment rat single cage fixing device according to the ... of the embodiment of the present invention,
In, mouse cage fixing device is closed；
Fig. 2 is the structural schematic diagram of Occupational exposure to magnetic fields experiment rat single cage fixing device according to the ... of the embodiment of the present invention,
In, mouse cage fixing device is in the open state；
Fig. 3 is planar structure and the rat representative moving position vertical view in radiation field of shortwave radiation field；
Fig. 4 is the rat radiation intensity analogue simulation figure that different location receives in radiation field, wherein：
4 (a) is field distribution sectional drawing of the rat in radiation field, and 4 (b) is radiation field intensity dosage simulation result；
Fig. 5 is that Rat hippocampus structure according to the ... of the embodiment of the present invention passes through 0,5,10 and 30mW/cm2Shortwave radiation
1st day Rat hippocampus structure chart afterwards；
Fig. 6 is that Rat hippocampus structure according to the ... of the embodiment of the present invention passes through 0,5,10 and 30mW/cm2Shortwave radiation
7th day Rat hippocampus structure chart afterwards；
Fig. 7 is that Rat hippocampus structure according to the ... of the embodiment of the present invention passes through 0,5,10 and 30mW/cm2Shortwave radiation
14th day Rat hippocampus structure chart afterwards；
Fig. 8 is that Rat hippocampus structure according to the ... of the embodiment of the present invention passes through 0,5,10 and 30mW/cm2Shortwave radiation
28th day Rat hippocampus structure chart afterwards；
Wherein, in Fig. 5 to Fig. 8, the lower left corner marks number 0,5,10 and 30 and respectively represents Normal group, 5mW/cm2
Group, 10mW/cm2Group and 30mW/cm2Group, arrow indicate the nerve cell that pyknosis contaminates deeply.
Reference numeral：
Mouse cage fixing device 100；
Mouse cage frame 10；Upper level board 11；Lower horizontal board 12；First vertical plate 13；Second vertical plate 14；
Gate baffle 20；
Controllable register 30；
Air hole 101；Installation through-hole 102；Avoid notch 103.
Specific implementation mode
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings.Below with reference to
The embodiment of attached drawing description is exemplary, it is intended to for explaining the present invention, and is not considered as limiting the invention, ability
The those of ordinary skill in domain can make changes, modifications, alterations, and variations to the above described embodiments within the scope of the invention.
Occupational exposure to magnetic fields experiment rat single cage fixing device according to the ... of the embodiment of the present invention is described in detail below in conjunction with the accompanying drawings
100。
Shown in referring to Figures 1 and 2, Occupational exposure to magnetic fields experiment rat single cage fixing device 100 according to the ... of the embodiment of the present invention
It may include 10, two gate baffles 20 of mouse cage frame and controllable register 30.
The cavity of cuboid is defined in mouse cage frame 10, when mouse cage fixing device 100 is normally placed, cavity can be along water
Square to extend and used as rat accommodation space.The both ends open of the length direction of mouse chamber, i.e., along the length direction of cavity
On, the both ends of cavity are opened wide respectively forms two open ends.Two gate baffles 20 movably with the both ends phase of mouse cage frame 10
Even, to open and close two open ends of mouse chamber.Specifically when in use, can be used as one of in two open ends into
Mouth end, another can be used as outlet end, to be conveniently operated.
It gates baffle 20 and mouse cage frame 10 is equipped with air hole 101, air hole 101 can be connected to cavity and the external world, make mouse
Inner air and outer air circulation may be implemented in cage fixing device 100, avoids sultry, reduces the interference of non-empirical factor, is conducive to the essence of experiment
Parasexuality.In the present invention, the installation position of air hole 101 is not done it is specifically limited, in addition in gate baffle 20 and mouse cage frame
It is arranged except air hole 101 simultaneously on 10, air hole 101 can also be only set on mouse cage frame 10.In addition, ventilative in order to improve
Air hole 101 can also be arranged in effect on controllable register 30.There is no particular/special requirement for the quantity of air hole 101, for example,
It can be five, ten or more etc., can be flexibly arranged according to pore size and ventilative intensity requirement etc..
Mouse cage frame 10 is equipped with installation through-hole 102, and installation through-hole 102 is connected to mouse chamber, and controllable register 30 is set in which can pull and push
In installation through-hole 102, mouse chamber is located in installation through-hole 102 in controllable register 30 and is divided into two sub- chambers along its length.
That is, controllable register 30 can be inserted in installation through-hole 102, it can also be extracted out out of installation through-hole 102, work as controllable register
30 when being not inserted into installation through-hole 102, a cavity generally chamber, when controllable register 30 is inserted into installation through-hole 102, mouse
Chamber can be conditioned baffle 30 along the length direction of itself and be separated into two sub- chambers.
Thus, it is possible to by controllable register 30, the big minor adjustment of rat accommodation space is realized.It is also possible to by multiple
Mouse cage fixing device 100 is stitched together, and the opening and closing of baffle 20 is gated by adjusting, realizes the big ditty of rat accommodation space
Section.I.e. in the present invention, the big ditty of rat accommodation space can be realized by gating the cooperation of baffle 20 and controllable register 30
Section.
Mouse cage fixing device 100 according to the ... of the embodiment of the present invention, by be arranged both ends open mouse cage frame 10 and can be with
The gate baffle 20 and controllable register 30 being movably connected therewith can flexibly realize the big minor adjustment of rat accommodation space, adjust
Flexibly, when specifically used, free-standing single cage structure may be used and use, it also can be according to uniform field range size by multiple single cages
Structural union uses, and homogeneous radiation is received to make study subject whole body be located in homogeneous radiation field, realizes miniaturization radiation
The homogeneous radiation of the single rat in source, improves the precision of experiment.Meanwhile the both ends open formula that mouse cage fixing device 100 has
Hole type structure, more meet experimental animal and get into the cave nature, easily facilitate animal and be packed into and fixed, operation is more convenient.
The device has been successfully applied in the biological effect research that high-frequency short waves radiation causes Brain Function of Rats and structural damage,
It is 140cm that minimum, which can meet radiation uniform field size,2Radiation source requirement, it is more smart than dose of radiation with the unused device stationary phase
Accuracy can be improved about 25.45%, find 10~30mW/cm2High-frequency short waves radiation can cause learning and memory in rats ability to decline；Brain
Electricity Functional is disorderly, is mainly shown as that the reduction of fast wave β power, slow wave δ and θ power increase；Hippocampal tissue structural damage.
As depicted in figs. 1 and 2, two gate baffles 20 can be moved vertically relative to mouse cage frame 10, controllable register
30 also can vertically move relative to mouse cage frame 10.Thus, it is possible to by moving up and down gate baffle 20 and controllable register
30 realize the opening and closing of rat accommodation space and big minor adjustment, more convenient operation.
Rat fixing device in the related technology is all made of upper opening type, and push-and-pull overhead gage, which easily exists, when sealing or so crushes
The problems such as rat-tail, it is inconvenient that animal is placed, and affects being widely popularized and being smoothed out for electromagnetic radiation biological effect research.Favorably
Ground, in the present invention, the middle part for gating the lower end of baffle 20 are equipped with evacuation notch 103, and evacuation notch 103 is connected to cavity.When
When placing rat into cavity, evacuation notch 103 can effectively avoid rat-tail, prevent from interfering door because rat-tail is long
The fixation of baffle 20 is controlled, while avoiding gating the closing of baffle 20 when rat-tail does not enter mouse intracavitary also and clipping to rat-tail, effectively
Solve the problems, such as to crush rat-tail in the presence of the relevant technologies, further ensure experiment be smoothed out and rat is put into more
It is smooth convenient.
Optionally, evacuation notch 103 can also be arranged in the middle part of the lower end of controllable register 30, with when adjusting space size
Rat-tail is avoided, after preferably ensureing that rat pierces corresponding sub- intracavitary, controllable register 30 can be smoothly inserted into, further
It prevents from crushing rat-tail, is more advantageous to successfully expansion experiment.
Size and shape for avoiding notch 103 do not do it is specifically limited, for example, evacuation notch 103 can be formed as half
Round hole, polygonal hole or irregular shape hole etc..Preferably, evacuation notch 103 is formed as square hole, the side of square hole
A length of 1 ± 0.5cm.The size is applicable to most of experiment, and applicability is wide and evacuation effect is good.
According to some embodiments of the present invention, the both ends of mouse cage frame 10 are respectively equipped with slot, and two gate baffles 20 can be taken out
It is located at slottingly in corresponding slot.Slot can be positioned and be limited to gate baffle 20 as a result, and gate baffle 20 is made to install
It is accurate and secured, it is particularly possible to be fixed on the length direction of mouse chamber, gate baffle 20 is avoided to be anticipated by the shock of rat
Outer opening, it is fixed and be not easy to shake off.Preferably, two gate baffles 20 are located at corresponding movably in a vertical direction
In slot, in operation, realization can be extracted out straight up and opened, be inserted into realize that closing, switching manipulation are more convenient simultaneously straight down
And convenient for manufacture.
To improve the fixed effect of controllable register 30, slot can also be set in the opposite interior sidewall surface of mouse chamber, make tune
Section baffle 30 can be not only fixed by installation through-hole 102, and can by slot to the both sides of controllable register 30 into
Row is fixed, and so that controllable register 30 is connect with mouse cage frame 10 more reliable.
Optionally, the horizontal distance that controllable register 30 and gate baffle 20 adjacent thereto are spaced is 5 ± 1cm.Change speech
It, the horizontal distance that neighbouring gate baffle 20 and controllable register 30 are spaced with controllable register 30 is 5 ± 1cm, that is, adjusts gear
Plate 30 is located substantially at the position of 5 ± 1cm of horizontal distance away from an open end, and regulating effect is good, can meet different size build
Rat, which is fixed, to be required.
According to some embodiments of the present invention, air hole 101 is formed as the round hole of a diameter of 1.5 ± 0.5cm.By
This, can preferably ensure that inner air and outer air circulates, and the shape of mouse cage fixing device 100 is more beautiful.As depicted in figs. 1 and 2,
The air hole 101 of multiple settings spaced apart is respectively equipped on mouse cage frame 10, gate baffle 20 and controllable register 30, mouse cage fixes dress
Set 100 air permeability effect it is excellent, while can also facilitate from from outside test in rat state, it is tentative good.
As depicted in figs. 1 and 2, mouse cage frame 10 may include level board 11, lower horizontal board 12, the first vertical plate 13 and second
Vertical plate 14.Upper level board 11 and lower horizontal board 12 are distinguished horizontally extending and are vertically spaced apart setting.The
One vertical plate 13 and the second vertical plate 14 vertically extend respectively and horizontally spaced open up is set.First vertical plate
13, upper level board 11, the second vertical plate 14 and lower horizontal board 12 are vertically connected and limit cavity successively.Upper level board 11,
It is respectively equipped with air hole 101 on one vertical plate 13 and the second vertical plate 14.Not only facilitate manufacture as a result, but also air permeability effect be good,
In addition, lower horizontal board 12 is tight non-porous, it can prevent experimental animal stool and urine in receiving radiative process from polluting instrument and equipment.
Some specific examples according to the present invention, lower horizontal board 12, upper level board 11, the first vertical plate 13, second are vertical
Plate 14, gate baffle 20 and controllable register 30 are acrylic board.Acrylic board, that is, poly (methyl methacrylate) plate, this kind of material are non-toxic and tasteless
And mechanical strength is higher, it is ensured that animal health simultaneously prevents it from biting, and the material do not interfere with electromagnetic wave propagation with
Distribution, and then reduce experiment interference factor.Further, the thickness of lower horizontal board 12 is 10 ± 0.2mm, and the thickness of remaining plate is
5±0.2mm.The bottom surface of mouse cage frame 10 is thicker as a result, and lap is slightly light, and the slightly light structure design in this thick and heavy upper end in bottom can
The stability for increasing cage, effectively prevent toppling over.
Optionally, the length of mouse cage frame 10 can be 20 ± 1cm, and width can be 7 ± 1cm, highly can be 8 ± 1cm.As a result,
The requirement for limiting rat voluntary activity can be met, and be unlikely to too to fetter.
A specific example of mouse cage fixing device 100 according to the ... of the embodiment of the present invention is shown in Fig. 1 and Fig. 2.Such as Fig. 1
Shown in Fig. 2, mouse cage fixing device 100 includes 10, two gate baffles 20 of mouse cage frame and controllable register 30, the formation of mouse cage frame 10
For porous cage, cavity that a centre is cuboid and both ends open are surrounded by four rectangular tablet stick to each others
Integral structure, wherein one in four tablets for no hole plate and thickness it is relatively thick, as mouse cage fixing device 100
Bottom surface, remaining three tablet is porous flat plate and thickness is relatively thin, as mouse cage fixing device 100 two sides and
Top surface.Two gate baffles 20 and controllable register 30 are located on mouse cage frame 10 in which can vertically pull and push.Tablet, gate baffle
20 and controllable register 30 on air hole 101 be a diameter of 1.5cm round hole.
The correlation test carried out using above-mentioned mouse cage fixing device 100 is described in detail below.
(A) fixed position of the rat in radiation field：Radiation field middle section is the rectangular region of rule, and both ends are not
Regular triangle region.If rat is not fixed free activity in radiation field, there is position 1~5 etc. in representative zone of action
(such as Fig. 3 shows).Obtain radiation field intensity distribution map that rat is subject in different location (such as Fig. 4 institutes using computer simulation
Show).By can be calculated：Rat is fixed on mouse cage fixing device at position 1, is fixed with the unused device and in place
It sets free activity at 1~5 to compare, the accuracy of suffered dose of radiation improves 25.45%.Therefore, in subsequent experimental
Rat is fixed at position 1 using the device and is radiated.
(B) shortwave radiation is carried out to rat：Two level male Wistar rat (the army for being 200 ± 20 grams by 100 weight
Thing Academy of Medical Sciences Experimental Animal Center provides), it is randomly divided into four groups：Normal group, 5mW/cm2Group, 10mW/cm2Group and
30mW/cm2Group, every group 25.Using the shortwave radiation source of Ministry of Industry and Information's TRE telecommunications research establishment, average power density is respectively 5,10 and
30mW/cm2Shortwave to rat radiate, radiated time 6min.Rat is placed in mouse cage fixing device and fixation postures.Control
Group carries out false radiation, that is, closes radiation source, and other conditions are the same.
(C) learning and memory in rats ability detects：System detectio learning and memory in rats ability is tested using Morris water mazes
Change.The system is made of stainless steel round pool (120 centimetres of diameter, 50 centimetres high), video camera, computer for analysis software.
20 centimetres of the depth of water is controlled when use, pond is divided into 4 quadrants, each quadrant pool wall centre position by 23 degrees centigrade of water temperature
For place of entry.Optionally 18 centimetres of diameter, high 18 centimetres dark platforms are placed in wherein 1 quadrant centre position.Peace above pond
Video camera is set, is connected with software systems, the continuous rats'swimming track that records is to be analyzed.
Each group rat acclimatization training 2 days before shortwave radiation are positioned for 1~4 day, 7 days, 14 days and 28 days after radiation
Navigation experiment.By rat towards pool wall respectively from 4 place of entry into the water, record it and find the time of platform in 60 seconds
(escape latency), using average value, that is, average escape latency of 4 quadrants as observation index.If not finding platform in 60 seconds
It is then denoted as 60 seconds, continues next quadrant experiment.It the results are shown in Table 1.
Influence (second) of 1 shortwave radiation of table to rat average escape latency
Remarks：Compared with the control group, * P<0.05, * * P<0.01
By result in table 1 it is found that 4 groups of rats are as experiment number increases, average escape latency is in certain period of time
In trend is gradually shortened.Compared with the control group, 5mW/cm2Group rat average escape latency obviously changes in nothing of each time point
Become, 10 and 30mW/cm2Group rat was obviously prolonged (P at 1~7 day<0.05), until without significant difference after 14 days.It can thus be appreciated that 10 Hes
30mW/cm2Shortwave radiation can lead to the damage of rat Spatial memory ability.
(D) detection of rat electroencephalogram：After irradiation 1 day, 7 days, 14 days and 28 days respectively, 1% penta bars are implemented to rat
It than appropriate sodium intraperitoneal anesthesia (0.5 milliliter/100 grams), causes rat in light anesthesia state, leads physiology record more using MP-150 and divide
Analysis system records and analyzes rat electroencephalogram.In the rat crown, middle section does not place an electrode after preserved skin, and left ear-lobe places reference
Electrode, needle electrode are fixed on subcutaneously, and continuous record 5 minutes analyzes the changed power feelings of 4 kinds of brain waves α, β, δ and θ respectively
Condition.2 be the results are shown in Table to table 5.
Influence (μ V of 2 shortwave radiation of table to the power of rat brain wave α2)
Influence (μ V of 3 shortwave radiation of table to the power of rat brain wave β2)
Influence (μ V of 4 shortwave radiation of table to the power of rat brain wave δ2)
Influence (μ V of 5 shortwave radiation of table to the power of rat brain wave θ2)
Remarks：Compared with the control group, * P<0.05, * * P<0.01
By result in table it is found that the α wave powers of 4 groups of rats are in equal no significant difference of each time point；β wave powers were at 1 day
Each radiation group reduces, and shows the lower change of its bigger power of dose of radiation, and it is gradual to extend each group difference with each time point
It reduces, until each group no significant difference at 28 days；θ and δ wave powers are in 5mW/cm2Group is without significantly raising, 10 and 30mW/cm2Group is apparent
It increases, with dosage positive correlation, extends radiation group with each time point and be gradually brought to normal level.Illustrate the shortwave of doses
Radiation can cause rat brain Electricity Functional disorderly, be mainly shown as 10 and 30mW/cm2Shortwave radiation can cause the reduction of fast wave β power, slowly
Wave δ and θ power increases.
(E) rat hippocampal Observation of Histological Structure：It is right by 4 groups of rats 1 day, 7 days, 14 days and 28 days after radiation
After rat implements 1% yellow Jackets intraperitoneal anesthesia (1.5 milliliters/100 grams), sacrificed by decapitation detaches cerebral hippocampal tissue, away from forebrain 6
Make coronal section (hippocampus position) at millimeter, it is conventional de- after taking 10% buffered formalin of latter half brain tissue to fix 1 week
Water, transparent, waxdip, embedding make 3 μ m-thick paraffin sections, and HE dyeing, om observation simultaneously photographs to record, as a result such as Fig. 5 to Fig. 8
It is shown.
Om observation is dyed it is found that control rats hippocampal formation dentate granule cell and the areas CA3 centrum by brain tissue
Cell arrangement is neat, and neuron morphology is full, and cytoplasm is uniform.Compared with the control group, 1 day when each radiation group without significantly changing
Become；5mW/cm at 7 days2There is slight pyknosis and contaminates deeply in the group a small amount of neuron of rat hippocampus, 10mW/cm2The neuron pyknosis of group is deep
Dye changes and increases, 30mW/cm2There is the neuron that a large amount of pyknosis contaminate deeply in group；Each radiation group neuron pyknosis is caught an illness deeply at 14 days
Become the trend for showing mitigation；The 28th day after to radiation, each radiation group neuron restores substantially to normal level.Show certain agent
The shortwave radiation of amount can cause brain tissue structural damage.
Other of mouse cage fixing device 100 according to the ... of the embodiment of the present invention constitute and operate the common skill for this field
It is known for art personnel, is not described in detail herein.
In the description of the present invention, it is to be understood that, term " length ", " thickness ", "upper", "lower", "front", "rear",
The orientation or positional relationship of the instructions such as "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is based on attached drawing institute
The orientation or positional relationship shown, is merely for convenience of description of the present invention and simplification of the description, and does not indicate or imply the indicated dress
It sets or element must have a particular orientation, with specific azimuth configuration and operation, therefore should not be understood as the limit to the present invention
System.
In addition, term " first ", " second " are used for description purposes only, it is not understood to indicate or imply relative importance
Or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or
Implicitly include at least one this feature.In the description of the present invention, the meaning of " plurality " is at least two, such as two, three
It is a etc., unless otherwise clearly defined and limited；Fisrt feature can be that the first and second features are direct in second feature "upper"
Contact or the first and second features are by intermediary mediate contact, unless otherwise specifically defined.
In the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " fixation " etc.
Term shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integral；Can be that machinery connects
It connects, can also be to be electrically connected or can communicate each other；It can be directly connected, can also indirectly connected through an intermediary, it can be with
It is the interaction relationship of the connection or two elements inside two elements, unless otherwise restricted clearly.For this field
For those of ordinary skill, the specific meanings of the above terms in the present invention can be understood according to specific conditions.
In the description of this specification, the description of reference term " embodiment " or " specific example " etc. means to combine the implementation
Example or example particular features, structures, materials, or characteristics described are included at least one embodiment or example of the invention.
In the present specification, a schematic expression of the above terms does not necessarily refer to the same embodiment or example.Moreover, description
Particular features, structures, materials, or characteristics may be combined in any suitable manner in any one or more of the embodiments or examples.
In addition, without conflicting with each other, those skilled in the art can by different embodiments described in this specification or
The feature of example and different embodiments or examples is combined.
Claims (8)
1. a kind of Occupational exposure to magnetic fields experiment rat single cage fixing device, which is characterized in that described device is arranged to be used for electromagnetism
The minimum 140cm of exposure range2Radiation uniform field in, described device includes：
Mouse cage frame, the cavity of cuboid is defined in the mouse cage frame, and the both ends open of the length direction of the cavity is described
Mouse cage is provided with the installation through-hole being connected to the cavity；
Two gate baffles, two gate baffles are movably connected with the both ends of the mouse cage frame to be opened and closed the cavity
Two open ends, be respectively equipped on the gate baffle and the mouse cage frame connection cavity with it is extraneous multiple ventilative
Hole；
Controllable register, the controllable register are located in the installation through-hole in which can pull and push, and the cavity is in the controllable register position
Two sub- chambers can be divided into along its length when in the installation through-hole,
Wherein, the mouse cage frame is arranged in the region of the uniform place covering of the electromagnetic radiation.
2. Occupational exposure to magnetic fields experiment rat single cage fixing device according to claim 1, which is characterized in that two doors
Control baffle and the controllable register are vertically moved relative to the mouse cage frame, the controllable register and gate gear
Be respectively equipped with the evacuation notch being connected to the cavity in the middle part of the lower end of plate, the evacuation notch be formed as the length of side be 1 ±
The square hole of 0.5cm.
3. Occupational exposure to magnetic fields experiment rat single cage fixing device according to claim 1, which is characterized in that the mouse cage frame
Both ends be respectively equipped with slot, two gate baffles are located in the corresponding slot in which can pull and push.
4. Occupational exposure to magnetic fields experiment rat single cage fixing device according to claim 1, which is characterized in that each described
Stomatal limiting value is the round hole of a diameter of 1.5 ± 0.5cm.
5. Occupational exposure to magnetic fields experiment rat single cage fixing device according to claim 1, which is characterized in that the adjusting gear
The horizontal distance that plate and the gate baffle adjacent thereto are spaced is 5 ± 1cm, and the controllable register is equipped at least one
The air hole.
6. Occupational exposure to magnetic fields experiment rat single cage fixing device according to any one of claims 1-5, which is characterized in that
The mouse cage frame includes：
Upper level board and lower horizontal board, the upper level board and lower horizontal board difference are horizontally extending and along vertical side
To setting spaced apart；
First vertical plate and the second vertical plate, first vertical plate and second vertical plate vertically extend respectively and
Horizontally spaced open up is set, first vertical plate, the upper level board, second vertical plate and the lower horizontal board
Be vertically connected and limit the cavity successively, the upper level board, first vertical plate and second vertical plate it is upper
It is respectively equipped with multiple air holes.
7. Occupational exposure to magnetic fields experiment rat single cage fixing device according to claim 6, which is characterized in that the lower level
Plate is the acrylic board that thickness is 10 ± 0.2mm, the upper level board, first vertical plate, second vertical plate, described
Gate baffle and the controllable register are respectively the acrylic board that thickness is 5 ± 0.2mm.
8. Occupational exposure to magnetic fields experiment rat single cage fixing device according to claim 1, which is characterized in that the mouse cage frame
Length be 20 ± 1cm, width be 7 ± 1cm, be highly 8 ± 1cm.
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| CN201611240094.5A CN106725977B (en) | 2016-12-28 | 2016-12-28 | Occupational exposure to magnetic fields experiment rat single cage fixing device |
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|---|---|---|---|
| CN201611240094.5A CN106725977B (en) | 2016-12-28 | 2016-12-28 | Occupational exposure to magnetic fields experiment rat single cage fixing device |
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| CN106725977A CN106725977A (en) | 2017-05-31 |
| CN106725977B true CN106725977B (en) | 2018-10-09 |
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| CN110074891A (en) * | 2018-01-25 | 2019-08-02 | 中国辐射防护研究院 | Irradiation test rat fixing device |
| CN109363795B (en) * | 2018-09-29 | 2020-07-31 | 东南大学 | Microwave irradiation device and method suitable for open field |
| CN111486978A (en) * | 2019-01-29 | 2020-08-04 | 中国人民解放军军事科学院军事医学研究院 | System and method for measuring animal anal temperature in real time under electromagnetic exposure environment |
| CN112773549A (en) * | 2019-11-07 | 2021-05-11 | 中国人民解放军军事科学院军事医学研究院 | Positionable rodent immobilization device for electromagnetic radiation exposure |
| CN111418518B (en) * | 2020-05-19 | 2021-09-10 | 河南省人民医院 | Water labyrinth device in experimental research of cerebral ischemic rat cognitive dysfunction |
| CN113243304B (en) * | 2021-06-24 | 2022-10-28 | 中国科学院深圳先进技术研究院 | Marmoset behaviourology experiment box |
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