CN106721060A - A kind of preparation method of soybean small peptide nanometer selenium chelate - Google Patents
A kind of preparation method of soybean small peptide nanometer selenium chelate Download PDFInfo
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Abstract
The present invention relates to a kind of preparation method of soybean small peptide nanometer selenium chelate, key step includes dregs of beans crushing, enzymolysis, press filtration, ultrafiltration, chelating, is spray-dried to obtain soybean small peptide nanometer selenium chelate.The method of the present invention with cheap, wide material sources dregs of beans be protein peptides source, with low toxicity, efficient nanometer selenium as selenium source, by nanometer selenium with it is small-peptide chelated, technological process is simple, low cost, is adapted to the industrial production of scale.The features such as product prepared by the present invention has safety and stability, directly absorption, strong bioactivity, lasting selenium-supply, can improve efficiency of feed utilization as animal feed additive, strengthen animal immunizing power and resistance against diseases, promote growth of animal.
Description
Technical field
The present invention relates to a kind of preparation method of feeding trace mineral supplement, more particularly to a kind of soybean small peptide nanometer selenium
The preparation method of chelate.
Background technology
Selenium is used as glutathione peroxidase（GSH-Px）Constituent, lipid peroxidation can be removed in vivo
Free radical intermediate product, prevents the lipid peroxidation of biomembrane, maintains the normal configuration and function of biomembrane；Selenium also participates in coenzyme
The synthesis of A and ubiquinone, plays an important role in tricarboxylic acid cycle and electron transfer process in vivo.Selenium is with selenocysteine
It is present in selenoprotein with two kinds of forms of selenomethionine, the Radical Metabolism, anti-oxidant of animal body is influenceed by selenoprotein
Function, immunologic function, reproductive function, Apoptosis and internal system etc. and play its biological function.During animal selenium deficiency
Auxotype muscular atrophy can occur, the initial stage may show as expiratory dyspnea, and skeletal muscle rigidity, cub occurs white muscle disease, and pig can also
There is trophism hepatonecrosis, neurological susceptibility during adults selenium deficiency then to disease increases, and reproductive capability obstacle easily occurs in dam
Deng.Inorganic selenium toxicity is big, and absorption rate is low, and Organic Selenium and nanometer selenium have small toxicity, are easy to absorption, efficient, high activity
It is preferable selenium-supply source etc. advantage.
Small peptide refers to dipeptides of the relative molecular weight between 180~1000Da, tripeptides and tetrapeptide.Numerous studies show,
There is small peptide mechanism of absorption in animal body, small peptide intactly can enter body-internal-circulation by protection of intestinal mucosal barrier cells.Animal 67%
Amino acid is absorbed in the form of small peptide, and remaining 33% ability is absorbed in the form of free amino acid (FAA).Small peptide is not only
Protein synthesis improves nitrogen frame, improves protein aggregate velocity, and with anti-oxidant, enhancing animal immunizing power, raising mineral
The physiological regulation functions such as matter absorption rate, promotion gut development, reduction diarrhoea generation.
Small peptide microelement chelate is emerging third generation trace mineral supplement, is easily absorbed by animal, biology effect
Valency is high, safe and efficient, microelement-supplementing, the life to animal while supplementing biologically active peptide, meeting nutrient protein needs
The healthy growth of particularly brood long plays very important effect, is the side of rear antibiotic epoch feed addictive development
To.But, the research and development both at home and abroad to small peptide microelement chelate is little at present, does not have mature technology, particularly has
The preparation method of soybean small peptide nanometer selenium chelate is closed there is not yet research report.
The content of the invention
A kind of preparation side of soybean small peptide nanometer selenium chelate that the purpose of the present invention is based on above-mentioned condition and proposes
Method, the method with Soybean Meal as primary raw material, process is simple, low cost, it is easy to implement, prepared soybean small peptide nanometer selenium chela
Compound is remarkably improved efficiency of feed utilization as trace mineral supplement, strengthens animal immunizing power and premunition, promotes animal life
It is long.
To achieve the above object, the present invention is realized using following technical scheme：A kind of soybean small peptide nanometer selenium chelating
The preparation method of thing, its processing step is as follows：
（1）Dregs of beans is crushed：Defatted soybean meal was crushed into 100 mesh sieves, bean cake powder was obtained；
（2）Enzymolysis：By step（1）The bean cake powder plus deionized water for obtaining are stirred and evenly mixed, and are configured to protein concentration for 8-10%（w/
w）Dregs of beans liquid, regulation dregs of beans liquid pH value adds protease to 8.0-9.0, after digesting 4-6h at a temperature of 50-55 DEG C, by enzyme
Solution liquid is warming up to 85 DEG C of enzymes 10 minutes of going out, that is, obtain dregs of beans enzymolysis liquid；
（3）Press filtration：By step（2）The dregs of beans enzymolysis liquid for obtaining is 200 mesh through plate and frame filter press press filtration, filter cloth mesh number, is pressed
Filtrate and filter cake, filter cake can be made into bean dregs feed after drying and crushing；
（4）Ultrafiltration：By step（3）The pressing filtering liquid for obtaining, for the ultra-filtration membrane device of 1000Da carries out ultrafiltration, is received through molecular cut off
Collection permeate, obtains soybean small peptide solution；
（5）Chelatropic reaction：To step（4）Nanometer selenium is added in the soybean small peptide solution for obtaining, the addition of nanometer selenium is soybean
Protein peptides amount in small peptide solution（In terms of protein content）1.5-3%, the pH value of solution is adjusted to 8.0~8.5, in 45-50
Chelatropic reaction is stirred at DEG C 1-1.5 hours, obtain soybean small peptide nanometer selenium chelatropic reaction liquid；
（6）Spray drying：By step（5）The soybean small peptide nanometer selenium chelatropic reaction liquid for obtaining is spray-dried, that is, obtain big
Beans small peptide nanometer selenium chelate.
Step（1）Described defatted soybean meal is the defatted soybean meal of protein content >=43%.
Step（2）Described protease is subtilopeptidase A, and enzyme activity is 200000-220000U/ ml, protease
Addition be the 1.0-1.5% of albumen quality in dregs of beans liquid.
Step（4）Described soybean small peptide solution is big more than 80% for small-molecular peptides account for the ratio of total protein in solution
Legumin peptide solution, the small-molecular peptides are peptide of the relative molecular weight between 180~1000Da.
Step（5）It is 1 by sodium selenite and citric acid that described nanometer selenium is in mass ratio:2 mixed grindings prepare gained.
Step（6）Intake air temperature is 180-220 DEG C during the spray drying, and air outlet temperature is 70-90 DEG C.
By implementing above-mentioned technical proposal, the present invention has following beneficial effect：
(1) modern biotechnology such as method of the present invention integrated application enzymolysis, ultrafiltration, molecule chelating and spray drying and existing
For process technology, simple with technological process, production efficiency is high, low cost, the characteristics of be adapted to industrial-scale production.
（2）The method of the present invention with cheap, wide material sources dregs of beans be protein peptides source, with low toxicity, efficient nanometer
Selenium is selenium source, by nanometer selenium with it is small-peptide chelated, small peptide is mutually promoted with the physiological function of nanometer selenium, Synergistic, gained produce
The features such as product have strong directly absorption, bioactivity, safety and stability, lasting selenium-supply, can significantly improve growth of animal performance, strengthen
The immunity and premunition of animal.
Specific embodiment
With reference to specific embodiment, the present invention is further detailed explanation, but embodiments of the present invention are simultaneously
It is not limited to the scope that this embodiment is represented.
Embodiment 1
（1）Dregs of beans is crushed：Weigh 100Kg defatted soybean meal powder（Crude protein content 45%）The mesh sieve of broken mistake 100, obtains bean cake powder 100Kg；
（2）Enzymolysis：By step（1）The bean cake powder for obtaining adds water deionized water 460L, stirs, and it is 8% to be configured to protein concentration
（w/w）Dregs of beans liquid, regulation dregs of beans liquid pH value adds the protease of albumen quality 1.0% in dregs of beans liquid, in 52 DEG C of temperature to 8.0
After lower enzymolysis 6h, enzymolysis liquid is warming up to 85 DEG C of enzymes 10 minutes of going out, that is, obtains dregs of beans enzymolysis liquid；
（3）Press filtration：By step（2）The dregs of beans enzymolysis liquid for obtaining is 200 mesh through plate and frame filter press press filtration, filter cloth mesh number, is pressed
Filtrate and filter cake, filter cake can be made into bean dregs feed after drying and crushing；
（4）Ultrafiltration：By step（3）The pressing filtering liquid for obtaining, for the ultra-filtration membrane device of 1000Da carries out ultrafiltration, is received through molecular cut off
Collection permeate, obtains soybean small peptide solution 292L；
（5）Chelatropic reaction：To step（4）Its protein peptides amount is added in the soybean small peptide solution for obtaining（In terms of protein content）
1.5% nanometer selenium, it is 1 by sodium selenite and citric acid that the nanometer selenium is in mass ratio:Ground after 2 mixing and prepare gained, will
The pH value of solution is adjusted to 8.0, stirring reaction 1.5 hours at 45 DEG C, obtains soybean small peptide nanometer selenium chelatropic reaction liquid 285L；
（6）Spray drying：By step（5）The soybean small peptide nanometer selenium chelatropic reaction liquid for obtaining is spray-dried, air inlet temperature
It is 200 DEG C to spend, and air outlet temperature is 80 DEG C, obtains soybean small peptide nanometer selenium chelate 37.2KG, and detection Se content is 0.177%.
Embodiment 2
（1）Dregs of beans is crushed：Weigh 100Kg defatted soybean meal powder（Crude protein content 45%）The mesh sieve of broken mistake 100, obtains bean cake powder 100Kg；
（2）Enzymolysis：By step（1）The bean cake powder for obtaining adds water deionized water 400L, stirs, and it is 9% to be configured to protein concentration
（w/w）Dregs of beans liquid, regulation dregs of beans liquid pH value adds the protease of albumen quality 1.2% in dregs of beans liquid, in 50 DEG C of temperature to 8.5
After lower enzymolysis 4h, enzymolysis liquid is warming up to 85 DEG C of enzymes 10 minutes of going out, that is, obtains dregs of beans enzymolysis liquid；
（3）Press filtration：By step（2）The dregs of beans enzymolysis liquid for obtaining is 200 mesh through plate and frame filter press press filtration, filter cloth mesh number, is pressed
Filtrate and filter cake, filter cake can be made into bean dregs feed after drying and crushing；
（4）Ultrafiltration：By step（3）The pressing filtering liquid for obtaining, for the ultra-filtration membrane device of 1000Da carries out ultrafiltration, is received through molecular cut off
Collection permeate, obtains soybean small peptide solution 233L；
（5）Chelatropic reaction：To step（4）Its protein peptides amount is added in the soybean small peptide solution for obtaining（In terms of protein content）
2.0% nanometer selenium, it is 1 by sodium selenite and citric acid that the nanometer selenium is in mass ratio:Ground after 2 mixing and prepare gained, will
The pH value of solution is adjusted to 8.5, stirring reaction 1.0 hours at 50 DEG C, obtains soybean small peptide nanometer selenium chelatropic reaction liquid 226L；
（6）Spray drying：By step（5）The soybean small peptide nanometer selenium chelatropic reaction liquid for obtaining is spray-dried, air inlet temperature
It is 210 DEG C to spend, and air outlet temperature is 90 DEG C, obtains soybean small peptide nanometer selenium chelate 35.6KG, and detection Se content is 0.244%.
Embodiment 3
（1）Dregs of beans is crushed：Weigh 100Kg defatted soybean meal powder（Crude protein content 45%）The mesh sieve of broken mistake 100, obtains bean cake powder 100Kg；
（2）Enzymolysis：By step（1）The bean cake powder for obtaining adds water deionized water 350L, stirs, and being configured to protein concentration is
10%（w/w）Dregs of beans liquid, regulation dregs of beans liquid pH value adds the protease of albumen quality 1.5% in dregs of beans liquid, at 53 DEG C to 8.5
At a temperature of digest 5h after, enzymolysis liquid is warming up to 85 DEG C of enzymes 10 minutes of going out, that is, obtain dregs of beans enzymolysis liquid；
（3）Press filtration：By step（2）The dregs of beans enzymolysis liquid for obtaining is 200 mesh through plate and frame filter press press filtration, filter cloth mesh number, is pressed
Filtrate and filter cake, filter cake can be made into bean dregs feed after drying and crushing；
（4）Ultrafiltration：By step（3）The pressing filtering liquid for obtaining, for the ultra-filtration membrane device of 1000Da carries out ultrafiltration, is received through molecular cut off
Collection permeate, obtains soybean small peptide solution 210L；
（5）Chelatropic reaction：To step（4）Its protein peptides amount is added in the soybean small peptide solution for obtaining（In terms of protein content）
2.5% nanometer selenium, it is 1 by sodium selenite and citric acid that the nanometer selenium is in mass ratio:Ground after 2 mixing and prepare gained, will
The pH value of solution is adjusted to 8.3, stirring reaction 1.0 hours at 45 DEG C, obtains soybean small peptide nanometer selenium chelatropic reaction liquid 198L；
（6）Spray drying：By step（5）The soybean small peptide nanometer selenium chelatropic reaction liquid for obtaining is spray-dried, air inlet temperature
It is 190 DEG C to spend, and air outlet temperature is 80 DEG C, obtains soybean small peptide nanometer selenium chelate 36.1KG, and detection Se content is 0.302%.
Embodiment 4
（1）Dregs of beans is crushed：Weigh 100Kg defatted soybean meal powder（Crude protein content 45%）The mesh sieve of broken mistake 100, obtains bean cake powder 100Kg；
（2）Enzymolysis：By step（1）The bean cake powder for obtaining adds water deionized water 400L, stirs, and it is 9% to be configured to protein concentration
（w/w）Dregs of beans liquid, regulation dregs of beans liquid pH value adds the protease of albumen quality 1.2% in dregs of beans liquid, in 50 DEG C of temperature to 8.5
After lower enzymolysis 6h, enzymolysis liquid is warming up to 85 DEG C of enzymes 10 minutes of going out, that is, obtains dregs of beans enzymolysis liquid；
（3）Press filtration：By step（2）The dregs of beans enzymolysis liquid for obtaining is 200 mesh through plate and frame filter press press filtration, filter cloth mesh number, is pressed
Filtrate and filter cake, filter cake can be made into bean dregs feed after drying and crushing；
（4）Ultrafiltration：By step（3）The pressing filtering liquid for obtaining, for the ultra-filtration membrane device of 1000Da carries out ultrafiltration, is received through molecular cut off
Collection permeate, obtains soybean small peptide solution 240L；
（5）Chelatropic reaction：To step（4）Its protein peptides amount is added in the soybean small peptide solution for obtaining（In terms of protein content）
3.0% nanometer selenium, it is 1 by sodium selenite and citric acid that the nanometer selenium is in mass ratio:Ground after 2 mixing and prepare gained, will
The pH value of solution is adjusted to 8.3, stirring reaction 1.5 hours at 48 DEG C, obtains soybean small peptide nanometer selenium chelatropic reaction liquid 232L；
（6）Spray drying：By step（5）The soybean small peptide nanometer selenium chelatropic reaction liquid for obtaining is spray-dried, air inlet temperature
It is 180 DEG C to spend, and air outlet temperature is 70 DEG C, obtains soybean small peptide nanometer selenium chelate 36.8KG, and detection Se content is 0.358%.
Embodiment 5
（1）Dregs of beans is crushed：Weigh 100Kg defatted soybean meal powder（Crude protein content 48%）The mesh sieve of broken mistake 100, obtains bean cake powder 100Kg；
（2）Enzymolysis：By step（1）The bean cake powder for obtaining adds water deionized water 433L, stirs, and it is 9% to be configured to protein concentration
（w/w）Dregs of beans liquid, regulation dregs of beans liquid pH value adds the protease of albumen quality 1.5% in dregs of beans liquid, in 55 DEG C of temperature to 8.5
After lower enzymolysis 4h, enzymolysis liquid is warming up to 85 DEG C of enzymes 10 minutes of going out, that is, obtains dregs of beans enzymolysis liquid；
（3）Press filtration：By step（2）The dregs of beans enzymolysis liquid for obtaining is 200 mesh through plate and frame filter press press filtration, filter cloth mesh number, is pressed
Filtrate and filter cake, filter cake can be made into bean dregs feed after drying and crushing；
（4）Ultrafiltration：By step（3）The pressing filtering liquid for obtaining, for the ultra-filtration membrane device of 1000Da carries out ultrafiltration, is received through molecular cut off
Collection permeate, obtains soybean small peptide solution 272L；
（5）Chelatropic reaction：To step（4）Its protein peptides amount is added in the soybean small peptide solution for obtaining（In terms of protein content）
2.0% nanometer selenium, it is 1 by sodium selenite and citric acid that the nanometer selenium is in mass ratio:Ground after 2 mixing and prepare gained, will
The pH value of solution is adjusted to 8.2, stirring reaction 1.5 hours at 45 DEG C, obtains soybean small peptide nanometer selenium chelatropic reaction liquid 258L；
（6）Spray drying：By step（5）The soybean small peptide nanometer selenium chelatropic reaction liquid for obtaining is spray-dried, air inlet temperature
It is 220 DEG C to spend, and air outlet temperature is 90 DEG C, obtains soybean small peptide nanometer selenium chelate 38.5KG, and detection Se content is 0.231%.
Embodiment 6
（1）Dregs of beans is crushed：Weigh 100Kg defatted soybean meal powder（Crude protein content 43%）The mesh sieve of broken mistake 100, obtains bean cake powder 100Kg；
（2）Enzymolysis：By step（1）The bean cake powder for obtaining adds water deionized water 330L, stirs, and being configured to protein concentration is
10%（w/w）Dregs of beans liquid, regulation dregs of beans liquid pH value adds the protease of albumen quality 1.2% in dregs of beans liquid, at 55 DEG C to 8.5
At a temperature of digest 5h after, enzymolysis liquid is warming up to 85 DEG C of enzymes 10 minutes of going out, that is, obtain dregs of beans enzymolysis liquid；
（3）Press filtration：By step（2）The dregs of beans enzymolysis liquid for obtaining is 200 mesh through plate and frame filter press press filtration, filter cloth mesh number, is pressed
Filtrate and filter cake, filter cake can be made into bean dregs feed after drying and crushing；
（4）Ultrafiltration：By step（3）The pressing filtering liquid for obtaining, for the ultra-filtration membrane device of 1000Da carries out ultrafiltration, is received through molecular cut off
Collection permeate, obtains soybean small peptide solution 208L；
（5）Chelatropic reaction：To step（4）Its protein peptides amount is added in the soybean small peptide solution for obtaining（In terms of protein content）
2.5% nanometer selenium, it is 1 by sodium selenite and citric acid that the nanometer selenium is in mass ratio:Ground after 2 mixing and prepare gained, will
The pH value of solution is adjusted to 8.2, stirring reaction 1.5 hours at 45 DEG C, obtains soybean small peptide nanometer selenium chelatropic reaction liquid 202L；
（6）Spray drying：By step（5）The soybean small peptide nanometer selenium chelatropic reaction liquid for obtaining is spray-dried, air inlet temperature
It is 200 DEG C to spend, and air outlet temperature is 80 DEG C, obtains soybean small peptide nanometer selenium chelate 34.5KG, and detection Se content is 0.294%.
The finished product obtained to above-described embodiment carries out Se content, crude protein content, peptide molecular weight size and efficacy detection.
Se content, crude protein content and peptide molecular weight size detection：Se content uses atomic absorption spectroscopy determination,
Crude protein content is determined using GB/T6432-94 methods, and peptide molecular weight size uses high-efficient gel filtration chromatography（HPLC）Method is surveyed
It is fixed, reference is done with known molecular amount protein.
Result such as table 1：
From table 1 it follows that in finished product obtained by embodiment Se content more than 0.1%, crude protein content 75% with
On, wherein small-molecular peptides of the relative molecular weight between 180-1000Da account for the ratio of total protein and reach more than 80%.
Efficacy detection：Du × the length of 35 ages in days × Dasanyuan hybridization piglet 150 is selected, by body weight is close, hereditary basis
Similar principle, is randomly divided into 5 treatment groups, and each treatment group sets 3 repetitions, and each repeats 10 pigs.By sodium selenite, greatly
Beans small peptide nanometer selenium chelate（The Dietary Selenium Levels determined according to Se content and experiment are needed to calculate corresponding addition）Point
It is not added in basal diet with two selenium levels of Dietary Selenium 0.30mg/kg, 0.50mg/kg, is made into 4 kinds of experiment daily rations.Basis
Daily ration is compared.Soybean small peptide nanometer selenium chelate is finished product prepared by embodiment 2, and Se content is 0.244%.Test in 2016
March in year is carried out in the permanent Thailand animal husbandry share subordinate Lingbi plant in Tianjin, experimental period 30d.
The results are shown in Table 2, table 3:
From Table 2, it can be seen that compared with selenium control group is not added with, adding soybean small peptide nanometer selenium chelate selenium-supply, Dietary Selenium is dense
Spend during for 0.3mg/kg, 0.5mg/kg, soybean small peptide nanometer selenium chelate group makes daily gain improve 14.50% respectively,
19.79%, feedstuff-meat ratio is reduced 8.86% and 15.82%.Compared with sodium selenite group, soybean small peptide nanometer selenium chelate is added
Selenium-supply, in 0.3mg/kg, soybean small peptide nanometer selenium chelate group makes daily gain improve 7.41% to daily ration selenium concentration, makes material meat
Than reducing 3.36%, in 0.5mg/kg, soybean small peptide nanometer selenium chelate group improves daily gain to daily ration selenium concentration
13.30%, feedstuff-meat ratio is reduced 10.14%.Soybean small peptide nanometer selenium chelate prepared by this explanation present invention can be significantly improved
The daily gain and reduction feed-weight ratio of piglet, promote growing for piglet.
From table 3 it is observed that compared with selenium control group is not added with, adding soybean small peptide nanometer selenium chelate selenium-supply, daily ration
When selenium concentration is 0.5mg/kg, soybean small peptide nanometer selenium chelate group makes the incidence of disease decline 12.02%, declines the death rate
37.03%.Compared with sodium selenite group, add soybean small peptide nanometer selenium chelate selenium-supply, daily ration selenium concentration in 0.3mg/kg,
Soybean small peptide nanometer selenium chelate group makes the death rate decline 10.30%, and when daily ration selenium concentration is in 0.5mg/kg, soybean small peptide is received
Rice selenium chelate group makes the death rate decline to a great extent 30.59%.Soybean small peptide nanometer selenium chelate prepared by this explanation present invention can change
The immunity function of kind piglet, significantly increases the premunition and anti-stress ability of weanling pig.
Claims (6)
1. a kind of preparation method of soybean small peptide nanometer selenium chelate, comprises the following steps：
（1）Dregs of beans is crushed：Defatted soybean meal was crushed into 100 mesh sieves, bean cake powder was obtained；
（2）Enzymolysis：By step（1）The bean cake powder plus deionized water for obtaining are stirred and evenly mixed, and are configured to protein concentration for 8-10%（w/
w）Dregs of beans liquid, regulation dregs of beans liquid pH value adds protease to 8.0-9.0, after digesting 4-6h at a temperature of 50-55 DEG C, by enzyme
Solution liquid is warming up to 85 DEG C of enzymes 10 minutes of going out, that is, obtain dregs of beans enzymolysis liquid；
（3）Press filtration：By step（2）The dregs of beans enzymolysis liquid for obtaining is 200 mesh through plate and frame filter press press filtration, filter cloth mesh number, is pressed
Filtrate and filter cake, filter cake can be made into bean dregs feed after drying and crushing；
（4）Ultrafiltration：By step（3）The pressing filtering liquid for obtaining, for the ultra-filtration membrane device of 1000Da carries out ultrafiltration, is received through molecular cut off
Collection permeate, obtains soybean small peptide solution；
（5）Chelatropic reaction：To step（4）Nanometer selenium is added in the soybean small peptide solution for obtaining, the addition of nanometer selenium is soybean
Protein peptides amount in small peptide solution（In terms of protein content）1.5-3%, the pH value of solution is adjusted to 8.0~8.5, in 45-50
Chelatropic reaction is stirred at DEG C 1-1.5 hours, obtain soybean small peptide nanometer selenium chelatropic reaction liquid；
（6）Spray drying：By step（5）The soybean small peptide nanometer selenium chelatropic reaction liquid for obtaining is spray-dried, that is, obtain big
Beans small peptide nanometer selenium chelate.
2. the preparation method of a kind of soybean small peptide nanometer selenium chelate according to claim 1, it is characterised in that：Step
（1）Described defatted soybean meal is the defatted soybean meal of protein content >=43%.
3. the preparation method of a kind of soybean small peptide nanometer selenium chelate according to claim 1, it is characterised in that：Step
（2）Described protease is subtilopeptidase A, and enzyme activity is 200000-220000U/ml, and the addition of protease is beans
The 1.0-1.5% of albumen quality in dregs of rice liquid.
4. the preparation method of a kind of soybean small peptide nanometer selenium chelate according to claim 1, it is characterised in that：Step
（4）Described soybean small peptide solution accounts for soybean protein peptide solution of the ratio of total protein in solution more than 80% for small-molecular peptides,
The small-molecular peptides are peptide of the relative molecular weight between 180~1000Da.
5. the preparation method of a kind of soybean small peptide nanometer selenium chelate according to claim 1, it is characterised in that：Step
（5）It is 1 by sodium selenite and citric acid that described nanometer selenium is in mass ratio:2 mixed grindings prepare gained.
6. the preparation method of a kind of soybean small peptide nanometer selenium chelate according to claim 1, it is characterised in that：Step
（6）Intake air temperature is 180-220 DEG C during the spray drying, and air outlet temperature is 70-90 DEG C.
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| CN108684963A (en) * | 2018-05-21 | 2018-10-23 | 河南广安生物科技股份有限公司 | Small-peptide chelated manganese of a kind of nanometer for feed addictive and preparation method thereof |
| CN110250318A (en) * | 2019-07-23 | 2019-09-20 | 湖南硒宝宝健康产业有限责任公司 | A kind of preparation method of selenium-rich soybean protein |
| CN110423791A (en) * | 2019-08-06 | 2019-11-08 | 广州大学 | A kind of reproducibility polypeptide and its preparation method and application |
| CN111302858A (en) * | 2020-03-09 | 2020-06-19 | 沃博特生物科技有限公司 | Chelation method of medium trace elements in optical carbon ribozyme |
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| CN113826764A (en) * | 2021-09-24 | 2021-12-24 | 江苏三仪生物工程有限公司 | Preparation method of soybean small peptide chelated iron |
| JP2022508780A (en) * | 2018-10-12 | 2022-01-19 | チャイナ ナショナル リサーチ インスティテュート オブ フード アンド ファーメンテーション インダストリーズ カンパニー リミテッド | Selenium chelated pea oligopeptide and its preparation method and application |
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| 赵红岩，等: "饲用大豆小肽生产工艺的研究", 《大豆科学》 * |
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| CN110250318B (en) * | 2019-07-23 | 2022-11-25 | 湖南硒宝宝健康产业有限责任公司 | Preparation method of selenium-rich soybean protein |
| CN110423791A (en) * | 2019-08-06 | 2019-11-08 | 广州大学 | A kind of reproducibility polypeptide and its preparation method and application |
| CN111302858A (en) * | 2020-03-09 | 2020-06-19 | 沃博特生物科技有限公司 | Chelation method of medium trace elements in optical carbon ribozyme |
| CN111616272A (en) * | 2020-07-01 | 2020-09-04 | 河北坤元饲料有限公司 | Lamb feed and preparation method thereof |
| CN113826764A (en) * | 2021-09-24 | 2021-12-24 | 江苏三仪生物工程有限公司 | Preparation method of soybean small peptide chelated iron |
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