CN106719845A - Entomopathogenic nematode powder and preparation method thereof - Google Patents
Entomopathogenic nematode powder and preparation method thereof Download PDFInfo
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- CN106719845A CN106719845A CN201710046453.1A CN201710046453A CN106719845A CN 106719845 A CN106719845 A CN 106719845A CN 201710046453 A CN201710046453 A CN 201710046453A CN 106719845 A CN106719845 A CN 106719845A
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- Prior art keywords
- nematode
- entomopathogenic nematode
- pulvis
- entomopathogenic
- gram
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- 241000244206 Nematoda Species 0.000 title claims abstract description 83
- 230000000967 entomopathogenic effect Effects 0.000 title claims abstract description 56
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 239000000843 powder Substances 0.000 title claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000000203 mixture Substances 0.000 claims abstract description 11
- 239000000725 suspension Substances 0.000 claims abstract description 11
- 239000003381 stabilizer Substances 0.000 claims abstract description 8
- 239000003795 chemical substances by application Substances 0.000 claims abstract 2
- 230000001717 pathogenic effect Effects 0.000 claims description 14
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical group OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 8
- 239000003112 inhibitor Substances 0.000 claims description 7
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000003638 chemical reducing agent Substances 0.000 claims description 6
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical group [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims description 4
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 235000011187 glycerol Nutrition 0.000 claims description 4
- 239000004302 potassium sorbate Substances 0.000 claims description 4
- 235000010241 potassium sorbate Nutrition 0.000 claims description 4
- 229940069338 potassium sorbate Drugs 0.000 claims description 4
- 229920001285 xanthan gum Polymers 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 229910021536 Zeolite Inorganic materials 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 2
- 239000012467 final product Substances 0.000 claims description 2
- 239000001530 fumaric acid Substances 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- -1 potassium ester Chemical class 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 2
- 239000010457 zeolite Substances 0.000 claims description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims 2
- 241000195493 Cryptophyta Species 0.000 claims 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 229920000609 methyl cellulose Polymers 0.000 claims 1
- 239000001923 methylcellulose Substances 0.000 claims 1
- 235000019260 propionic acid Nutrition 0.000 claims 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 125000000185 sucrose group Chemical group 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 10
- 238000003860 storage Methods 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 3
- 239000000575 pesticide Substances 0.000 abstract description 2
- 238000009776 industrial production Methods 0.000 abstract 1
- 230000003449 preventive effect Effects 0.000 abstract 1
- 238000007873 sieving Methods 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 description 10
- 239000000470 constituent Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 3
- 229940084030 carboxymethylcellulose calcium Drugs 0.000 description 3
- JXKPEJDQGNYQSM-UHFFFAOYSA-M sodium propionate Chemical compound [Na+].CCC([O-])=O JXKPEJDQGNYQSM-UHFFFAOYSA-M 0.000 description 3
- 239000004324 sodium propionate Substances 0.000 description 3
- 235000010334 sodium propionate Nutrition 0.000 description 3
- 229960003212 sodium propionate Drugs 0.000 description 3
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 229940072056 alginate Drugs 0.000 description 2
- 235000010443 alginic acid Nutrition 0.000 description 2
- 229920000615 alginic acid Polymers 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- LDCRTTXIJACKKU-ONEGZZNKSA-N dimethyl fumarate Chemical compound COC(=O)\C=C\C(=O)OC LDCRTTXIJACKKU-ONEGZZNKSA-N 0.000 description 2
- 229960004419 dimethyl fumarate Drugs 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000003628 erosive effect Effects 0.000 description 2
- 244000000383 Allium odorum Species 0.000 description 1
- 235000018645 Allium odorum Nutrition 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 241000500097 Heterorhabditis indica Species 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Toxicology (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of pesticides, and particularly relates to entomopathogenic nematode powder and a preparation method thereof. The composition comprises the following components in percentage by weight: 20-40% of entomopathogenic nematode suspension with the concentration of 50-500 ten thousand per gram, 0.2-1.0% of water-retaining agent, 0.2-1.0% of stabilizing agent, 0.1-0.5% of mildew preventive, 40-50% of carrier and 10-30% of water. The preparation method comprises mixing the above materials, and sieving. The entomopathogenic nematode powder is convenient to use, long in low-temperature storage period, convenient to transport and high in survival rate. The preparation method is simple and convenient to operate and suitable for industrial production.
Description
Technical field
The present invention relates to technical field of pesticide, and in particular to a kind of entomopathogenic nematode pulvis and preparation method thereof.
Background technology
Entomopathogenic nematode preparation is a kind of ergastic biological control factor for growing up in 20th century, is the special of insect
The property changed insect parasite.The Si Shi sections for especially being contacted with fungal component and heterorhabditis indica, be easy for workers to culture, breeding potential
Height, host range is wide, almost can parasitic all targeted insects, easily application, pressure to sprayer unit and with various chemical agricultures
Medicine is used in mixed way and is barely affected, the advantages of to vertebrate, plant and safe other non-target organisms.Therefore it is dirty in agricultural chemicals
Dye is increasingly serious, today that pest resistance to insecticide quickly grows, and entomopathogenic nematode preparation has turned into compeling for Agricultural Sustainable Development
It is essential and wants, paid much attention to by international biological and ecological methods to prevent plant disease, pests, and erosion field, and has turned into one of the focus in current world biological and ecological methods to prevent plant disease, pests, and erosion field.
It is to expand China's entomopathogenic nematode at present that entomopathogenic nematode preparation can not be stored for a long time under the conditions of low cost
The major obstacle of formulation application.The storage of the nematode preparation of commercial production, it is desirable to low cost and make nematode be in steady quality,
Under conditions of suitable applications, this purpose is reached, it is necessary to suppress the activity of nematode, with the lipid and glycogen that prevent it from containing in vivo
Deposit declines.Past traditional method is to be stored in after nematode is cleaned in the water of low temperature ventilation or mixed with sponge, activated carbon etc.
Low tempertaure storage.But, above-mentioned storage procedures is not suitable for being commercialized, industrializing requirement and the needs of preparation, it is difficult to carry out commodity
Change.Therefore, the preferable nematode preparation of relatively stable, quality has progressively been developed in recent years, i.e., with activated carbon, polyacrylamide,
Alginate jelly makes nematode partially dried by materials such as clays, and activity is metabolized in holddown with reducing its, so that
Nematode is maintained.The form of preparation has mixed bait, the polysaccharide being made with paddy or peanut skin etc. of alginate capsule, tablet, nematode
Absorption pad, peat, polyether-polyurethane sponge, vermiculite etc. mix with nematode, and nematode is discharged by water when using, application
Sprayer or irrigate is applied, but these preparations or is unfavorable for long-term storage, or blocks spraying apparatus, or relatively costly, uncomfortable
Used in commercialization development ＆ production and field.
The content of the invention
It is an object of the invention to provide a kind of entomopathogenic nematode pulvis, pulvis entomopathogenic nematode pulvis user
Just, the low tempertaure storage phase is long, and transport facility, survival rate is high.
The present invention also aims to provide a kind of preparation method of entomopathogenic nematode pulvis, preparation method operation letter
It is single, convenient, it is suitable to industrialized production.
In order to realize the above object the present invention is adopted the following technical scheme that：
A kind of entomopathogenic nematode pulvis, it is composed of the following components by weight percentage：Concentration is the insect of/gram of 50-500 ten thousand
Pathogenic nematode suspension 20-40%, water-loss reducer 0.2-1.0%, stabilizer 0.2-1.0%, mould inhibitor 0.1-0.5%, carrier 40-50%,
Water 10-30%.
Optionally, the water-loss reducer is the mixture of one or more in glycerine, xanthans, carboxymethylcellulose calcium.
Optionally, the stabilizer is the mixture of one or more in sucrose, glucose, trehalose, sorbierite.
Optionally, the mould inhibitor is one or more in potassium sorbate, sodium propionate, the potassium ester of fumaric acid two, formaldehyde
Mixture.
Optionally, the carrier is one or more in diatomite, white carbon, soluble starch, natural zeolite powder
Mixture.
Optionally, in the entomopathogenic nematode pulvis, the content of entomopathogenic nematode is/gram of 25-100 ten thousand.
The preparation method of above-mentioned entomopathogenic nematode pulvis, by water-loss reducer, stabilizer, mould inhibitor, entomopathogenic nematode
Suspension and water are well mixed, obtain entomopathogenic nematode mixed liquor；Then after carrier and entomopathogenic nematode mixed liquor being mixed,
Sieved, obtained final product entomopathogenic nematode pulvis；The concentration of entomopathogenic nematode is 40 in gained entomopathogenic nematode mixed liquor
/ gram of ten thousand/gram -250 ten thousand.
Optionally, the screening and choosing is sieved with 20 mesh sieve.
Entomopathogenic nematode pulvis of the invention, is preserved using carrier adsorption nematode so that entomopathogenic nematode is in storing
During dehydration enter resting state, with reduce preserve during energy and material consumption, finally extend entomopathogenic nematode
Shelf life；Water-loss reducer and stabilizer are added in product, it is therefore intended that enter stable state after ensureing nematode Anhydrous dormant, it is to avoid
Excessive dehydration is dead, store activity in product transporting procedures and infect activity to improve；Mould inhibitor is added in product, can effectively be changed
Mould and influence of other spoilage organisms to product in kind entomopathogenic nematode transporting procedures, reduce the death rate.
/ gram of nematode levels 250,000 ~ 1,000,000/gram in entomopathogenic nematode pulvis of the invention, water content be 50 ~
60%, the low tempertaure storage phase is long, can be stored 3 months under the conditions of 10 DEG C, and nematode survival rate is high, and survival rate is still after 3 months
More than 80%.Said preparation transport facility, product quality stability is high, easy to use, can be irrigated together with water, Henan, Hebei,
Preventing and treating fragrant-flowered garlic maggot works well on the ground such as Ningxia.
Preparation method of the invention, it is simple, convenient, workable, it is suitable to industrialized production.
Specific embodiment
Embodiment 1
200 grams of the entomopathogenic nematode suspension of 5000000/gram, 2 grams of xanthans, 2 grams of trehalose, 1 gram of potassium sorbate, water 295
Gram, whole mixing and stirrings obtain entomopathogenic nematode mixed liquor（/ gram of active constituent content 2,000,000）.By Insect Pathogenic
Nematode mixed liquor is sufficiently mixed with 500 grams of white carbons, crosses 20 mesh sieves, obtains entomopathogenic nematode pulvis.Active constituent content 100
Ten thousand/gram, water content 50% is stored 3 months at 10 DEG C, and survival rate is 80.58%.
Embodiment 2
250 grams of the entomopathogenic nematode suspension of 3000000/gram, 4 grams of glycerine, 4 grams of sucrose, 2 grams of dimethyl fumarate, water 260
Gram, whole mixing and stirrings obtain entomopathogenic nematode mixed liquor（/ gram of active constituent content 1,440,000）.By Insect Pathogenic
Nematode mixed liquor is sufficiently mixed with 480 grams of diatomite, crosses 20 mesh sieves, obtains entomopathogenic nematode pulvis.Active constituent content 75
Ten thousand/gram, water content 52% is stored 3 months at 10 DEG C, and survival rate is 84.25%.
Example 3
300 grams of the entomopathogenic nematode suspension of 2000000/gram, 6 grams of carboxymethylcellulose calcium, 4 grams of glucose, 3 grams of sodium propionate,
237 grams of water, whole mixing and stirrings obtain entomopathogenic nematode mixed liquor（/ gram of active constituent content 1,090,000）.By elder brother
Worm pathogenic nematode mixed liquor is sufficiently mixed with 330 grams of diatomite, 120 grams of soluble starches, crosses 20 mesh sieves, obtains Insect Pathogenic line
Worm pulvis./ gram of active constituent content 600,000, water content 55% is stored 3 months at 10 DEG C, and survival rate is 89.92%.
Embodiment 4
300 grams of the entomopathogenic nematode suspension of 2000000/gram, 6 grams of xanthans, 6 grams of glucose, 3 grams of potassium sorbate, water 235
Gram, whole mixing and stirrings obtain entomopathogenic nematode mixed liquor（/ gram of active constituent content 1,090,000）.By Insect Pathogenic
Nematode mixed liquor is sufficiently mixed with 450 grams of white carbons, crosses 20 mesh sieves, obtains entomopathogenic nematode pulvis.Active constituent content 60
Ten thousand/gram, water content 55% is stored 3 months at 10 DEG C, and survival rate is 89.67%.
Embodiment 5
350 grams of the entomopathogenic nematode suspension of 1000000/gram, 8 grams of carboxymethylcellulose calcium, 8 grams of trehalose, 4 grams of sodium propionate,
210 grams of water, whole mixing and stirrings obtain entomopathogenic nematode mixed liquor（/ gram of active constituent content 600,000）.By insect
Pathogenic nematode mixed liquor is sufficiently mixed with 420 grams of white carbons, crosses 20 mesh sieves, obtains entomopathogenic nematode pulvis.Active ingredient contains
350,000/gram are measured, water content 58% is stored 3 months at 10 DEG C, and survival rate is 90.2%.
Embodiment 6
400 grams of the entomopathogenic nematode suspension of 62.5 ten thousand/gram, 10 grams of glycerine, 10 grams of sorbierite, 5 grams of dimethyl fumarate,
175 grams of water, whole mixing and stirrings obtain entomopathogenic nematode mixed liquor（/ gram of active constituent content 420,000）.By insect
Pathogenic nematode mixed liquor is sufficiently mixed with 400 grams of white carbons, crosses 20 mesh sieves, obtains entomopathogenic nematode pulvis.Active ingredient contains
250,000/gram are measured, water content 60% is stored 3 months at 10 DEG C, and survival rate is 93.33%.
Claims (8)
1. a kind of entomopathogenic nematode pulvis, it is characterised in that composed of the following components by weight percentage：Concentration is 50-500
Entomopathogenic nematode the suspension 20-40%, water-loss reducer 0.2-1.0%, stabilizer 0.2-1.0%, mould inhibitor 0.1- of ten thousand/gram
0.5%, carrier 40-50%, water 10-30%.
2. worm pathogenic nematode pulvis according to claim 1, it is characterised in that the water-loss reducer is glycerine, xanthans, carboxylic
The mixture of one or more in methylcellulose.
3. worm pathogenic nematode pulvis according to claim 1, it is characterised in that the stabilizer is sucrose, glucose, sea
The mixture of one or more in algae sugar, sorbierite.
4. worm pathogenic nematode pulvis according to claim 1, it is characterised in that the mould inhibitor is potassium sorbate, propionic acid
The mixture of one or more in sodium, the potassium ester of fumaric acid two, formaldehyde.
5. worm pathogenic nematode pulvis according to claim 1, it is characterised in that the carrier be diatomite, white carbon, can
The mixture of one or more in soluble starch, natural zeolite powder.
6. worm pathogenic nematode pulvis according to claim 1, it is characterised in that in the entomopathogenic nematode pulvis, elder brother
The content of worm pathogenic nematode is/gram of 25-100 ten thousand.
7. the preparation method of any described entomopathogenic nematode pulvis of a kind of claim 1-6, it is characterised in that by water conservation
Agent, stabilizer, mould inhibitor, entomopathogenic nematode suspension and water are well mixed, and obtain entomopathogenic nematode mixed liquor；Then will carry
After body is mixed with entomopathogenic nematode mixed liquor, sieved, obtained final product entomopathogenic nematode pulvis；Gained entomopathogenic nematode is mixed
The concentration for closing entomopathogenic nematode in liquid is/gram of 400,000/gram -250 ten thousand.
8. the preparation method of entomopathogenic nematode pulvis according to claim 7, it is characterised in that the screening and choosing uses 20
Mesh sieve is sieved.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710046453.1A CN106719845B (en) | 2017-01-22 | 2017-01-22 | Entomopathogenic nematode powder and preparation method thereof |
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|---|---|---|---|
| CN201710046453.1A CN106719845B (en) | 2017-01-22 | 2017-01-22 | Entomopathogenic nematode powder and preparation method thereof |
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| Publication Number | Publication Date |
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| CN106719845A true CN106719845A (en) | 2017-05-31 |
| CN106719845B CN106719845B (en) | 2019-08-13 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108142415A (en) * | 2017-12-26 | 2018-06-12 | 河北农业大学 | Method for preparing entomopathogenic nematode capsule |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108142415A (en) * | 2017-12-26 | 2018-06-12 | 河北农业大学 | Method for preparing entomopathogenic nematode capsule |
| CN108142415B (en) * | 2017-12-26 | 2020-09-04 | 河北农业大学 | Method for preparing entomopathogenic nematode capsule |
| CN108849937A (en) * | 2018-07-11 | 2018-11-23 | 四川利尔作物科学有限公司 | A kind of low temperature resistant diquat dibromide aqua |
| CN109845730A (en) * | 2019-04-03 | 2019-06-07 | 河北农业大学 | Powder containing entomopathogenic nematodes |
| CN109845730B (en) * | 2019-04-03 | 2022-02-11 | 河北农业大学 | Powder containing entomopathogenic nematodes |
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| CN106719845B (en) | 2019-08-13 |
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