CN103053619A - Antibacterial, anti-dry and anti-adhesion method of dead insects infected by entomopathogenetic nematodes - Google Patents
Antibacterial, anti-dry and anti-adhesion method of dead insects infected by entomopathogenetic nematodes Download PDFInfo
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- CN103053619A CN103053619A CN2011103198542A CN201110319854A CN103053619A CN 103053619 A CN103053619 A CN 103053619A CN 2011103198542 A CN2011103198542 A CN 2011103198542A CN 201110319854 A CN201110319854 A CN 201110319854A CN 103053619 A CN103053619 A CN 103053619A
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Abstract
The method performs surface treatment on dead insects resulted from infection of entomopathogenetic nematodes (entomopathogenetic nematodes and insect complexes) through a three-step method. Firstly, anti-dry and antibacterial treatment is performed on the insect complexes infected by the entomopathogenetic nematodes; the insect complexes infected by the entomopathogenetic nematodes are immersed in a mixed solution of kaolin, glycerin soil and water; then further surface treatment is carried out in mixed powder of kaolin starch and vermiculite; and finally, edible pigment is sprayed to make the surface treated dead insects infected by the entomopathogenetic nematodes red color. The surface treated dead insects infected by the entomopathogenetic nematodes can release the entomopathogenetic nematodes normally. Surface treated dead insect agents solve the problems of adhesion and decay during a transport process and guarantees release rate of the entomopathogenetic nematodes. The method is simple and practical, is helpful for large-scale application of the dead insects infected by the entomopathogenetic nematodes.
Description
Technical field
The present invention relates to a kind of insect corpse to the infected insect pathogenic nematode and carry out antibiotic, resist drying and Anti-adhesive, be conducive to entomopathogenic nematode in insect bodies growth and transportation and the field of the insect corpse of infected insect pathogenic nematode use.
Background technology
Entomopathogenic nematode is called for short EPNs (Entomopathogenic Nematodes), is the focus in the in recent years biological control insect research, is mainly used in controlling soil insect, to reach the purpose of protection specified plant.It comprises that mainly genus steinernema (Steinernema) and different little bar under the Nematoda belong to (Heterorhabditis).
Entomopathogenic nematode has the following advantages as a kind of desirable biological insecticides: obligatory parasitism, host range be wide, have initiatively search capability; Can enter in the host insect body from natural opening (such as mouthpart, valve and anus), wound or the intersegmental membrane of host insect, and can kill rapidly host insect; Has the advantage that self duplication is fast, be easy to genetic improvement: can cultivate in a large number so that synthetic medium is low-cost: to people and animals, plant and beneficial organism safety, exempted the registration production and selling in more American-European countries.
The common entomopathogenic nematode of directly using solid culture or liquid cultivating method production carries out the control of harmful insect both at home and abroad, but there is the phenomenon of comparatively obvious vigor and toxicity decline in the entomopathogenic nematode under artificial the cultivation.Add coercing of the natural factor such as arid, ultraviolet under the further decline that inevitably causes the nematode vigor in the operating process such as cleaning, storage of artificial culture method middle and later periods, the outdoor conditions, often cause the lability of entomopathogenic nematode product insecticidal effect.If the polypide of infected insect pathogenic nematode directly is manured into soil, then can keep the virulence of entomopathogenic nematode.But the insect corpse of infected insect pathogenic nematode rots easily and is inter-adhesive, is difficult to storage, transportation and field and uses, again because the dry stress factors that waits has a strong impact on the growth of entomopathogenic nematode in insect bodies.These factors have limited the insect corpse of infected insect pathogenic nematode greatly applying aspect the control harmful insect.
Summary of the invention
The purpose of this invention is to provide the method that a kind of insect corpse to the infected insect pathogenic nematode carries out antibiotic, resist drying and Anti-adhesive.1), the insect corpse of infected insect pathogenic nematode surface moisturizing antimicrobial treatment the present invention carries out dressing to the insect of being infected by entomopathogenic nematode by two-step method:, form the gluey film of one deck on the greater wax moth surface, in both can absorbing air the moisture epidermis that guarantees greater wax moth can shrinkage, can guarantee gas permeability again, so that the entomopathogenic nematode in the greater wax moth body has sufficient oxygen; 2), on first step basis, the insect corpse of the infected insect pathogenic nematode that soaked is placed in the mixed-powder of kaolin, vermiculite, starch and fully wraps up, at low temperatures 4-6 ℃ the storage certain hour after, the insect corpse of infected insect pathogenic nematode still can discharge a large amount of entomopathogenic nematodes, and the greater wax moth after the surface treatment adhesion can not occur in transportation, the problem such as rot, can significantly improve the insect corpse service time of infected insect pathogenic nematode, promote its direct application and popularization in the control of field harmful insect.
That the present invention carries out is antibiotic, the exploration of the method for resist drying and Anti-adhesive specifically may further comprise the steps:
The insect corpse of first step infected insect pathogenic nematode is antibiotic, resist drying is processed.(bactericide: kaolin: clay: glycerine: water=0-100: 0-100: 0-100: 0-100: 0-100), it is even fully to be mixed to join the internal layer mixed solution.Place this mixed solution fully to soak the insect corpse of infected insect pathogenic nematode.
The Anti-adhesive of the insect corpse of second step infected insect pathogenic nematode.Obtain the vermiculite powder with 60 orders and 100 purposes combination sieve.And the vermiculite powder mixed with kaolin, starch, three's ratio is 0-100: 0-100: 0-100, it is even fully to be mixed, and before use with mixed-powder in baking oven more than 105 ℃ of baking 2h.The insect corpse of the infected insect pathogenic nematode of solution immersion in the above-mentioned first step is put into the container of containing mixed-powder, fully rock, make powder wrapped to the insect corpse, then with sieve powder is separated with the insect polypide, namely obtain the insect corpse of the good infected insect pathogenic nematode of surface treatment.
The 3rd step: surface colour is processed.Food coloring is dissolved in the water, uses the atomizers spray polypide, the insect corpse of surface-treated infected insect pathogenic nematode is taken on a red color, have certain warning colouration, comparatively safe again.
Embodiment:
That the insect corpse of 1 pair of infected insect pathogenic nematode of embodiment carries out is antibiotic, behind resist drying and the Anti-adhesive, the burst size of entomopathogenic nematode, insect corpse two all interior entomopathogenic nematode amounts that discharge of infected insect pathogenic nematode reach 196119/gram, illustrate that the insect corpse shows that processing rear entomopathogenic nematode can normally discharge.
Table 1 is tested starch group nematode release conditions for the first time
Embodiment 2 repeats embodiment 1, after the insect corpse of infected insect pathogenic nematode carried out antibiotic, resist drying and Anti-adhesive, the burst size of entomopathogenic nematode, insect corpse two all interior entomopathogenic nematode amounts that discharge of infected insect pathogenic nematode reach 156133/gram, illustrate that again the insect corpse shows that processing rear entomopathogenic nematode can normally discharge.
Table 2 is tested starch group nematode release conditions for the second time
Claims (6)
1. antibiotic, the resist drying of the insect corpse of an a kind of infected insect pathogenic nematode and adhesion preventing method, it is characterized in that the insect corpse of infected insect pathogenic nematode is carried out surface treatment, and spray food coloring reaches antibiotic, anti-drying and prevents inter-adhesive purpose for warning.
2. according to greater wax moth corpse claimed in claim 1 surface treatment, it is characterized in that carrying out surface treatment at the insect body surface with mixed solution, mixed solution composition and ratio are bactericide: kaolin: clay: glycerine: water=0-100: 0-100: 0-100: 0-100: 0-100.
3. according to the surface treatment of the insect corpse of claim 1 and 2 described infected insect pathogenic nematodes, it is characterized in that processing with vermiculite, kaolin, starch mixture in insect surfaces, its ratio is vermiculite, kaolin, starch mixture=(0-100): (0-100): (0-100).
4. according to claim 1,2 and 3 described, it is characterized in that the insect corpse surface treatment of infected insect pathogenic nematode, use first " bactericide, kaolin, clay, G ﹠ W " mixed solution to carry out surface treatment, carry out again the further surface treatment of vermiculite, kaolin, starch mixed-powder.
5. according to the surface treatment of the insect corpse of claim 1,2,3,4 described infected insect pathogenic nematodes, it is characterized in that the insect corpse that mixed solution (bactericide, kaolin, clay, glycerine, water) and mixed-powder (vermiculite, kaolin, starch) were processed sprays food coloring, is used for warning.
6. according to the surface treatment of the insect corpse of claim 1,2,3,4 and 5 described infected insect pathogenic nematodes, it is characterized in that anti-drying, the antimicrobial treatment of the insect corpse of infected insect pathogenic nematode, Anti-adhesive and spray food coloring and process.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011103198542A CN103053619A (en) | 2011-10-20 | 2011-10-20 | Antibacterial, anti-dry and anti-adhesion method of dead insects infected by entomopathogenetic nematodes |
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| CN2011103198542A CN103053619A (en) | 2011-10-20 | 2011-10-20 | Antibacterial, anti-dry and anti-adhesion method of dead insects infected by entomopathogenetic nematodes |
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| CN103053619A true CN103053619A (en) | 2013-04-24 |
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| CN2011103198542A Pending CN103053619A (en) | 2011-10-20 | 2011-10-20 | Antibacterial, anti-dry and anti-adhesion method of dead insects infected by entomopathogenetic nematodes |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719845A (en) * | 2017-01-22 | 2017-05-31 | 河南省济源白云实业有限公司 | Entomopathogenic nematode powder and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2002087321A2 (en) * | 2001-04-30 | 2002-11-07 | Rutgers, The State University | Apparatus and method for mass production of insecticidal nematodes |
| US6524601B1 (en) * | 2001-06-08 | 2003-02-25 | The United States Of America As Represented By The Secretary Of Agriculture | Formulated arthropod cadavers for pest suppression |
| CN1928065A (en) * | 2006-01-24 | 2007-03-14 | 中国科学院东北地理与农业生态研究所 | Industrial high power breeding method for entomopathogenic nematode alive body |
| US7374773B1 (en) * | 2003-12-04 | 2008-05-20 | The United States As Represented By The Secretary Of Agriculture | Application of entomopathogenic nematode-infected cadavars from hard-bodied arthropods for insect suppression |
-
2011
- 2011-10-20 CN CN2011103198542A patent/CN103053619A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002087321A2 (en) * | 2001-04-30 | 2002-11-07 | Rutgers, The State University | Apparatus and method for mass production of insecticidal nematodes |
| US6524601B1 (en) * | 2001-06-08 | 2003-02-25 | The United States Of America As Represented By The Secretary Of Agriculture | Formulated arthropod cadavers for pest suppression |
| US7374773B1 (en) * | 2003-12-04 | 2008-05-20 | The United States As Represented By The Secretary Of Agriculture | Application of entomopathogenic nematode-infected cadavars from hard-bodied arthropods for insect suppression |
| CN1928065A (en) * | 2006-01-24 | 2007-03-14 | 中国科学院东北地理与农业生态研究所 | Industrial high power breeding method for entomopathogenic nematode alive body |
Non-Patent Citations (1)
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| 谢钦铭 等: "昆虫病原线虫在害虫防治中应用的研究进展", 《江西科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719845A (en) * | 2017-01-22 | 2017-05-31 | 河南省济源白云实业有限公司 | Entomopathogenic nematode powder and preparation method thereof |
| CN106719845B (en) * | 2017-01-22 | 2019-08-13 | 河南省济源白云实业有限公司 | Entomopathogenic nematode powder and preparation method thereof |
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Application publication date: 20130424 |